AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045613.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045613.01.T01 MTR_8g075290 92.135 89 6 1 1 89 1 88 3.67e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045613.01.T01 AT4G35980 58.427 89 35 2 1 89 1 87 2.04e-29 100

Find 25 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAACTTCTCTAATTAAT+AGG 0.220533 8:-61760749 MsG0880045613.01.T01:CDS
GTTTCAGCTAAATTATAAAA+AGG 0.298464 8:-61761063 MsG0880045613.01.T01:intron
TCCCTATTAATTAGAGAAGT+TGG 0.353531 8:+61760747 None:intergenic
GCCAACTTCTCTAATTAATA+GGG 0.360365 8:-61760748 MsG0880045613.01.T01:CDS
AAAATTTGTGATTGCAAGGA+TGG 0.400175 8:-61761009 MsG0880045613.01.T01:CDS
TGCAGCATCTCCCGAGGCAA+TGG 0.407315 8:-61760712 MsG0880045613.01.T01:CDS
GGATGACTCGCCCATTGCCT+CGG 0.414680 8:+61760701 None:intergenic
TAATCTTGCCGTTCCTGAGC+TGG 0.441007 8:+61760680 None:intergenic
GCAGCATCTCCCGAGGCAAT+GGG 0.454758 8:-61760711 MsG0880045613.01.T01:CDS
GATGACTCGCCCATTGCCTC+GGG 0.464021 8:+61760702 None:intergenic
TTTCTGATGCGTTCAGTCGA+AGG 0.485607 8:+61762688 None:intergenic
ATTCAAAATTTGTGATTGCA+AGG 0.486628 8:-61761013 MsG0880045613.01.T01:CDS
TGATTGCAAGGATGGGAAGT+AGG 0.501741 8:-61761001 MsG0880045613.01.T01:CDS
GGACTCGGCCATGTGATTTG+AGG 0.508194 8:-61760980 MsG0880045613.01.T01:intron
CAAGGATGGGAAGTAGGACT+CGG 0.540501 8:-61760995 MsG0880045613.01.T01:CDS
CGTTCAGTCGAAGGCCTCGA+TGG 0.572639 8:+61762697 None:intergenic
AAATTTGTGATTGCAAGGAT+GGG 0.573450 8:-61761008 MsG0880045613.01.T01:CDS
ATGGGCGAGTCATCCAGCTC+AGG 0.577264 8:-61760693 MsG0880045613.01.T01:CDS
AACGATGAAGGATGAACAAG+AGG 0.579391 8:-61762650 MsG0880045613.01.T01:intron
TGAATAATTGTACAACGTGT+CGG 0.581731 8:+61761031 None:intergenic
CGAGTCATCCAGCTCAGGAA+CGG 0.587000 8:-61760688 MsG0880045613.01.T01:CDS
TCGGGAGATGCTGCAGCGTG+TGG 0.595356 8:+61760720 None:intergenic
ACACGCTGCAGCATCTCCCG+AGG 0.615860 8:-61760718 MsG0880045613.01.T01:CDS
AAATGTAACCTCAAATCACA+TGG 0.661853 8:+61760972 None:intergenic
TGCTGTCACAATCACCATCG+AGG 0.702290 8:-61762711 MsG0880045613.01.T01:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTGTTTTTTTTTTT+TGG - Chr8:61762477-61762496 MsG0880045613.01.T01:intron 10.0%
!! AATTTCTCATATATAACTTA+TGG - Chr8:61761178-61761197 MsG0880045613.01.T01:intron 15.0%
!! TAATGTAAGTCAATTAAAAA+TGG + Chr8:61761357-61761376 None:intergenic 15.0%
!! TGAGTATTAATAAACAAAAT+TGG + Chr8:61760863-61760882 None:intergenic 15.0%
!! AAAATAAGTCAAAACAATAC+AGG + Chr8:61761498-61761517 None:intergenic 20.0%
!! CCAACATATATCTTTATATA+TGG + Chr8:61761523-61761542 None:intergenic 20.0%
!! CCATATATAAAGATATATGT+TGG - Chr8:61761520-61761539 MsG0880045613.01.T01:intron 20.0%
!! GTTTCAGCTAAATTATAAAA+AGG - Chr8:61762313-61762332 MsG0880045613.01.T01:intron 20.0%
!!! AAGAAGATTTCTTTTTGAAA+TGG + Chr8:61761951-61761970 None:intergenic 20.0%
!!! AATAAACTTGAATCTAAAAC+AGG - Chr8:61760982-61761001 MsG0880045613.01.T01:CDS 20.0%
!!! AATTCTGTTATTTTCTCATT+AGG - Chr8:61762445-61762464 MsG0880045613.01.T01:intron 20.0%
!!! ATAAACTTGAATCTAAAACA+GGG - Chr8:61760983-61761002 MsG0880045613.01.T01:CDS 20.0%
!!! ATTTTAAGCAAAATAGGAAA+GGG + Chr8:61762192-61762211 None:intergenic 20.0%
!!! GAAAAGACATACTAATTTTT+CGG + Chr8:61760945-61760964 None:intergenic 20.0%
!!! TATCATATGATGTTTCTATA+TGG - Chr8:61762501-61762520 MsG0880045613.01.T01:intron 20.0%
! AAGAAATGGAATGAAAGAAT+AGG + Chr8:61760757-61760776 None:intergenic 25.0%
! AATTAAGATCCCTAAGATTT+AGG - Chr8:61761911-61761930 MsG0880045613.01.T01:intron 25.0%
! ATTAAGATCCCTAAGATTTA+GGG - Chr8:61761912-61761931 MsG0880045613.01.T01:intron 25.0%
! ATTCAAAATTTGTGATTGCA+AGG - Chr8:61762363-61762382 MsG0880045613.01.T01:intron 25.0%
! ATTTAGCTGAAACAAAATTC+AGG + Chr8:61762306-61762325 None:intergenic 25.0%
! CAAAGTATGTTAATCATAAG+CGG + Chr8:61760791-61760810 None:intergenic 25.0%
! GTTAATTGATTACTATCTGT+GGG - Chr8:61762245-61762264 MsG0880045613.01.T01:intron 25.0%
! TAATCTTACGATTAGTGTTA+CGG - Chr8:61762142-61762161 MsG0880045613.01.T01:intron 25.0%
! TCAAATAAGCCAATCTAAAT+AGG + Chr8:61761100-61761119 None:intergenic 25.0%
! TGTTAATTGATTACTATCTG+TGG - Chr8:61762244-61762263 MsG0880045613.01.T01:intron 25.0%
! TTAATTGATTACTATCTGTG+GGG - Chr8:61762246-61762265 MsG0880045613.01.T01:intron 25.0%
! TTAGGGATCTTAATTACTAA+GGG + Chr8:61761906-61761925 None:intergenic 25.0%
! TTTGGAAATTAAACACAAGA+TGG + Chr8:61761983-61762002 None:intergenic 25.0%
!! AGTATTTCAAGTGTGATTTA+AGG - Chr8:61762043-61762062 MsG0880045613.01.T01:intron 25.0%
!! ATCGAGATTTTAAGCAAAAT+AGG + Chr8:61762198-61762217 None:intergenic 25.0%
!! GATTTTAAGCAAAATAGGAA+AGG + Chr8:61762193-61762212 None:intergenic 25.0%
!! TTTTAAGCAAAATAGGAAAG+GGG + Chr8:61762191-61762210 None:intergenic 25.0%
!!! AATTTTTTAGCCAATAACTG+AGG - Chr8:61761555-61761574 MsG0880045613.01.T01:intron 25.0%
!!! ATACTAATTTTTCGGTTTTG+AGG + Chr8:61760937-61760956 None:intergenic 25.0%
!!! CTATTTTGGCAAAACAAATT+GGG - Chr8:61761648-61761667 MsG0880045613.01.T01:intron 25.0%
!!! TAGGTGTTATAATCATTTTC+AGG - Chr8:61762592-61762611 MsG0880045613.01.T01:intron 25.0%
!!! TCTATTTTGGCAAAACAAAT+TGG - Chr8:61761647-61761666 MsG0880045613.01.T01:intron 25.0%
!!! TTTTTGTTTCCTTCTTCTAT+AGG - Chr8:61761714-61761733 MsG0880045613.01.T01:intron 25.0%
AAAATTTGTGATTGCAAGGA+TGG - Chr8:61762367-61762386 MsG0880045613.01.T01:intron 30.0%
AAATGTAACCTCAAATCACA+TGG + Chr8:61762407-61762426 None:intergenic 30.0%
AAATTTGTGATTGCAAGGAT+GGG - Chr8:61762368-61762387 MsG0880045613.01.T01:intron 30.0%
AAGAAGAAAAAGAACGATGA+AGG - Chr8:61760714-61760733 MsG0880045613.01.T01:CDS 30.0%
AATAACATCTCCTCAGTTAT+TGG + Chr8:61761568-61761587 None:intergenic 30.0%
AGCATAATCCCTAAATCTTA+GGG + Chr8:61761923-61761942 None:intergenic 30.0%
ATGATTATAACACCTATGTC+AGG + Chr8:61762588-61762607 None:intergenic 30.0%
ATTTGAAAGCCTATAGAAGA+AGG + Chr8:61761726-61761745 None:intergenic 30.0%
CGGATTTGAACAATAAGAAA+TGG + Chr8:61760771-61760790 None:intergenic 30.0%
CTTAGGGATCTTAATTACTA+AGG + Chr8:61761907-61761926 None:intergenic 30.0%
CTTCTATAGGCTTTCAAATT+TGG - Chr8:61761727-61761746 MsG0880045613.01.T01:intron 30.0%
GCCAACTTCTCTAATTAATA+GGG - Chr8:61762628-61762647 MsG0880045613.01.T01:intron 30.0%
GTTTCTATATGGAAGATGAA+GGG - Chr8:61762512-61762531 MsG0880045613.01.T01:intron 30.0%
TCCCTATTAATTAGAGAAGT+TGG + Chr8:61762632-61762651 None:intergenic 30.0%
TGAATAATTGTACAACGTGT+CGG + Chr8:61762348-61762367 None:intergenic 30.0%
TGATAACTAGTAGTGAAACT+GGG - Chr8:61761054-61761073 MsG0880045613.01.T01:CDS 30.0%
TGCCAACTTCTCTAATTAAT+AGG - Chr8:61762627-61762646 MsG0880045613.01.T01:intron 30.0%
! AAAAATGGATTCAAAGACCT+TGG + Chr8:61761342-61761361 None:intergenic 30.0%
! GGGCAATCTTTTAATGTTAA+GGG - Chr8:61761074-61761093 MsG0880045613.01.T01:intron 30.0%
! TGGGCAATCTTTTAATGTTA+AGG - Chr8:61761073-61761092 MsG0880045613.01.T01:intron 30.0%
! TGTTTCTATATGGAAGATGA+AGG - Chr8:61762511-61762530 MsG0880045613.01.T01:intron 30.0%
! TTTGAGGTTACATTTGATGA+AGG - Chr8:61762412-61762431 MsG0880045613.01.T01:intron 30.0%
!! AATCATTTTCAGGAGATTTC+AGG - Chr8:61762602-61762621 MsG0880045613.01.T01:intron 30.0%
!! AATCCCATTTTTGTGGAATT+GGG - Chr8:61761830-61761849 MsG0880045613.01.T01:intron 30.0%
!! GGTCTTTTACATTGATGATT+TGG + Chr8:61762285-61762304 None:intergenic 30.0%
!! TAATCCCATTTTTGTGGAAT+TGG - Chr8:61761829-61761848 MsG0880045613.01.T01:intron 30.0%
!!! TAATTTTTCGGTTTTGAGGA+AGG + Chr8:61760933-61760952 None:intergenic 30.0%
AAAATCGTAGGATCTTAGGA+TGG + Chr8:61761870-61761889 None:intergenic 35.0%
AAGTATAGTCGCAAAATCGT+AGG + Chr8:61761882-61761901 None:intergenic 35.0%
AATTAAACACAAGATGGACG+AGG + Chr8:61761977-61761996 None:intergenic 35.0%
ACTTGTGCAACATGAATAAG+GGG + Chr8:61761315-61761334 None:intergenic 35.0%
ATTAAACACAAGATGGACGA+GGG + Chr8:61761976-61761995 None:intergenic 35.0%
CAAAATAGATAGCTTCGCAT+GGG + Chr8:61761636-61761655 None:intergenic 35.0%
CTGATAACTAGTAGTGAAAC+TGG - Chr8:61761053-61761072 MsG0880045613.01.T01:CDS 35.0%
GACTTGTGCAACATGAATAA+GGG + Chr8:61761316-61761335 None:intergenic 35.0%
GAGCATAATCCCTAAATCTT+AGG + Chr8:61761924-61761943 None:intergenic 35.0%
TCTCCCAATTCCACAAAAAT+GGG + Chr8:61761836-61761855 None:intergenic 35.0%
TGTTAAGGGCCTATTTAGAT+TGG - Chr8:61761088-61761107 MsG0880045613.01.T01:intron 35.0%
TTCTCCCAATTCCACAAAAA+TGG + Chr8:61761837-61761856 None:intergenic 35.0%
! CATGAGATTTTGTCGTCATT+TGG + Chr8:61762001-61762020 None:intergenic 35.0%
! GAATCTAAAACAGGGTAATG+AGG - Chr8:61760991-61761010 MsG0880045613.01.T01:CDS 35.0%
! TGACTTGTGCAACATGAATA+AGG + Chr8:61761317-61761336 None:intergenic 35.0%
!! CGCATATAATCCCATTTTTG+TGG - Chr8:61761823-61761842 MsG0880045613.01.T01:intron 35.0%
!! GGAAGGAAAAGTATTGAAGT+TGG + Chr8:61760916-61760935 None:intergenic 35.0%
AACATGAATAAGGGGCTGTT+TGG + Chr8:61761307-61761326 None:intergenic 40.0%
AACGATGAAGGATGAACAAG+AGG - Chr8:61760726-61760745 MsG0880045613.01.T01:CDS 40.0%
AGGATCTTAGGATGGAAATC+AGG + Chr8:61761862-61761881 None:intergenic 40.0%
AGTCAAAACAATACAGGCAC+TGG + Chr8:61761492-61761511 None:intergenic 40.0%
CAAATGCGAAAGTAAGACCT+TGG + Chr8:61761411-61761430 None:intergenic 40.0%
CCAAAATAGATAGCTTCGCA+TGG + Chr8:61761637-61761656 None:intergenic 40.0%
GTCAAAACAATACAGGCACT+GGG + Chr8:61761491-61761510 None:intergenic 40.0%
TCGCAAAATCGTAGGATCTT+AGG + Chr8:61761874-61761893 None:intergenic 40.0%
! ATGCGTAGTCTTAATCGTTG+AGG + Chr8:61761808-61761827 None:intergenic 40.0%
! CCATGCGAAGCTATCTATTT+TGG - Chr8:61761634-61761653 MsG0880045613.01.T01:intron 40.0%
! TCAAAACAATACAGGCACTG+GGG + Chr8:61761490-61761509 None:intergenic 40.0%
! TGCGTAGTCTTAATCGTTGA+GGG + Chr8:61761807-61761826 None:intergenic 40.0%
ATGTTGCACAAGTCACACCA+AGG - Chr8:61761322-61761341 MsG0880045613.01.T01:intron 45.0%
CCATCTCTCTAACCTGACAT+AGG - Chr8:61762573-61762592 MsG0880045613.01.T01:intron 45.0%
CCTATGTCAGGTTAGAGAGA+TGG + Chr8:61762576-61762595 None:intergenic 45.0%
GATGTTTCCTGTGAACAGCA+GGG + Chr8:61761465-61761484 None:intergenic 45.0%
TCACACGACACACGAACATT+AGG + Chr8:61761382-61761401 None:intergenic 45.0%
TGATTGCAAGGATGGGAAGT+AGG - Chr8:61762375-61762394 MsG0880045613.01.T01:intron 45.0%
TTTCTGATGCGTTCAGTCGA+AGG + Chr8:61760691-61760710 None:intergenic 45.0%
CAAGGATGGGAAGTAGGACT+CGG - Chr8:61762381-61762400 MsG0880045613.01.T01:intron 50.0%
GGATGTTTCCTGTGAACAGC+AGG + Chr8:61761466-61761485 None:intergenic 50.0%
GTGTCGTGTGAATAGCACCA+AGG - Chr8:61761391-61761410 MsG0880045613.01.T01:intron 50.0%
TAATCTTGCCGTTCCTGAGC+TGG + Chr8:61762699-61762718 None:intergenic 50.0%
TGCTGTCACAATCACCATCG+AGG - Chr8:61760665-61760684 MsG0880045613.01.T01:CDS 50.0%
!! AAACAATACAGGCACTGGGG+AGG + Chr8:61761487-61761506 None:intergenic 50.0%
CGAGTCATCCAGCTCAGGAA+CGG - Chr8:61762688-61762707 MsG0880045613.01.T01:five_prime_UTR 55.0%
GGACTCGGCCATGTGATTTG+AGG - Chr8:61762396-61762415 MsG0880045613.01.T01:intron 55.0%
TTTCCTGTGAACAGCAGGGC+TGG + Chr8:61761461-61761480 None:intergenic 55.0%
AAGCCAGCCCTGCTGTTCAC+AGG - Chr8:61761455-61761474 MsG0880045613.01.T01:intron 60.0%
ATGGGCGAGTCATCCAGCTC+AGG - Chr8:61762683-61762702 MsG0880045613.01.T01:five_prime_UTR 60.0%
CGTTCAGTCGAAGGCCTCGA+TGG + Chr8:61760682-61760701 None:intergenic 60.0%
GATGACTCGCCCATTGCCTC+GGG + Chr8:61762677-61762696 None:intergenic 60.0%
GCAGCATCTCCCGAGGCAAT+GGG - Chr8:61762665-61762684 MsG0880045613.01.T01:exon 60.0%
GGATGACTCGCCCATTGCCT+CGG + Chr8:61762678-61762697 None:intergenic 60.0%
TGCAGCATCTCCCGAGGCAA+TGG - Chr8:61762664-61762683 MsG0880045613.01.T01:exon 60.0%
ACACGCTGCAGCATCTCCCG+AGG - Chr8:61762658-61762677 MsG0880045613.01.T01:exon 65.0%
TCGGGAGATGCTGCAGCGTG+TGG + Chr8:61762659-61762678 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 61760650 61762748 61760650 ID=MsG0880045613.01;Name=MsG0880045613.01
Chr8 mRNA 61760650 61762748 61760650 ID=MsG0880045613.01.T01;Parent=MsG0880045613.01;Name=MsG0880045613.01.T01;_AED=0.50;_eAED=0.52;_QI=80|0.5|0.33|1|0|0|3|0|89
Chr8 exon 61760650 61760803 61760650 ID=MsG0880045613.01.T01:exon:23645;Parent=MsG0880045613.01.T01
Chr8 exon 61760981 61761078 61760981 ID=MsG0880045613.01.T01:exon:23644;Parent=MsG0880045613.01.T01
Chr8 exon 61762651 61762748 61762651 ID=MsG0880045613.01.T01:exon:23643;Parent=MsG0880045613.01.T01
Chr8 five_prime_UTR 61762669 61762748 61762669 ID=MsG0880045613.01.T01:five_prime_utr;Parent=MsG0880045613.01.T01
Chr8 CDS 61762651 61762668 61762651 ID=MsG0880045613.01.T01:cds;Parent=MsG0880045613.01.T01
Chr8 CDS 61760981 61761078 61760981 ID=MsG0880045613.01.T01:cds;Parent=MsG0880045613.01.T01
Chr8 CDS 61760650 61760803 61760650 ID=MsG0880045613.01.T01:cds;Parent=MsG0880045613.01.T01
Gene Sequence

>MsG0880045613.01.T01

ATGAAGGATGAACAAGAGCTAAATTATAAAAAGGAAGCTGCTGCCGACACGTTGTACAATTATTCAAAATTTGTGATTGCAAGGATGGGAAGTAGGACTCGGCCATGTGATTTGAGGTGTTATAATCATTTTCAGGAGATTTCAGGCATGCCAACTTCTCTAATTAATAGGGAAACACCACACGCTGCAGCATCTCCCGAGGCAATGGGCGAGTCATCCAGCTCAGGAACGGCAAGATTAGATAAAGCAGACAGTTTTCGAGCAATATAG

Protein sequence

>MsG0880045613.01.T01

MKDEQELNYKKEAAADTLYNYSKFVIARMGSRTRPCDLRCYNHFQEISGMPTSLINRETPHAAASPEAMGESSSSGTARLDKADSFRAI*