AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013219.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013219.01.T02 MTR_3g436100 60.606 66 26 0 1 66 32 97 5.99e-25 91.3
MsG0380013219.01.T02 MTR_8g022430 54.098 61 26 2 6 66 34 92 5.08e-16 68.2
MsG0380013219.01.T02 MTR_7g044920 42.424 66 36 1 3 66 29 94 4.51e-15 65.9
MsG0380013219.01.T02 MTR_6g017150 50.820 61 27 2 6 66 35 92 4.69e-15 65.5
MsG0380013219.01.T02 MTR_6g017170 45.902 61 31 2 6 66 34 92 1.40e-11 56.2
MsG0380013219.01.T01 MTR_3g436100 62.887 97 36 0 1 97 1 97 3.52e-40 131
MsG0380013219.01.T01 MTR_8g022430 48.454 97 45 3 1 97 1 92 3.56e-24 90.1
MsG0380013219.01.T01 MTR_5g464350 66.129 62 21 0 1 62 1 62 1.07e-22 84.3
MsG0380013219.01.T01 MTR_5g464490 74.545 55 14 0 1 55 1 55 2.77e-22 83.2
MsG0380013219.01.T01 MTR_7g044920 44.444 99 48 3 1 97 1 94 9.22e-21 81.6
MsG0380013219.01.T01 MTR_6g017150 44.898 98 47 4 1 97 1 92 5.53e-20 79.3
MsG0380013219.01.T01 MTR_0112s0050 63.492 63 22 1 1 63 1 62 4.82e-19 75.1
MsG0380013219.01.T01 MTR_6g017170 44.330 97 49 3 1 97 1 92 5.76e-19 76.6
MsG0380013219.01.T01 MTR_5g464390 60.656 61 24 0 1 61 1 61 1.44e-18 73.9
MsG0380013219.01.T01 MTR_0416s0030 58.462 65 27 0 1 65 1 65 4.59e-18 72.4
MsG0380013219.01.T01 MTR_5g464590 58.730 63 24 2 1 61 1 63 9.69e-18 71.6
MsG0380013219.01.T01 MTR_7g056803 40.816 98 53 3 1 97 1 94 5.07e-17 71.6
MsG0380013219.01.T01 MTR_5g464540 55.385 65 28 1 1 65 1 64 4.72e-16 67.4
MsG0380013219.01.T01 MTR_7g044980 41.237 97 47 5 1 97 1 87 3.45e-15 67.4
MsG0380013219.01.T01 MTR_8g022400 39.175 97 54 3 1 97 1 92 5.47e-15 66.6
MsG0380013219.01.T01 MTR_0093s0090 57.895 57 23 1 1 57 1 56 1.60e-14 63.5
MsG0380013219.01.T01 MTR_8g022420 40.816 98 50 4 1 97 1 91 1.61e-14 65.5
MsG0380013219.01.T01 MTR_3g067540 41.748 103 47 4 1 97 1 96 1.87e-14 65.1
MsG0380013219.01.T01 MTR_3g067555 41.748 103 47 4 1 97 1 96 1.87e-14 65.1
MsG0380013219.01.T01 MTR_7g056817 41.000 100 51 5 1 97 1 95 3.53e-14 64.3
MsG0380013219.01.T01 MTR_3g067430 37.255 102 53 5 1 97 1 96 5.30e-14 63.9
MsG0380013219.01.T01 MTR_3g067437 39.216 102 47 4 1 97 1 92 1.70e-13 62.4
MsG0380013219.01.T01 MTR_3g067270 41.237 97 50 4 1 97 1 90 4.97e-13 61.6
MsG0380013219.01.T01 MTR_3g467750 41.237 97 50 4 1 97 1 90 4.97e-13 61.6
MsG0380013219.01.T01 MTR_6g036620 42.857 98 47 5 1 97 1 90 6.16e-13 61.2
MsG0380013219.01.T01 MTR_0112s0040 83.333 36 5 1 1 35 1 36 1.35e-12 58.2
MsG0380013219.01.T01 MTR_3g438170 80.000 35 7 0 1 35 1 35 2.80e-12 57.0
MsG0380013219.01.T01 MTR_3g438140 80.000 35 7 0 1 35 1 35 2.80e-12 57.0
MsG0380013219.01.T01 MTR_3g067535 39.604 101 50 4 1 97 1 94 2.24e-11 57.0
MsG0380013219.01.T03 MTR_3g436100 59.016 61 25 0 1 61 37 97 2.22e-22 84.3
MsG0380013219.01.T03 MTR_8g022430 54.098 61 26 2 1 61 34 92 5.22e-16 67.8
MsG0380013219.01.T03 MTR_6g017150 50.820 61 27 2 1 61 35 92 4.57e-15 65.5
MsG0380013219.01.T03 MTR_7g044920 42.857 63 34 1 1 61 32 94 3.07e-14 63.5
MsG0380013219.01.T03 MTR_6g017170 45.902 61 31 2 1 61 34 92 1.31e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATTATGTTCAGAACTAAT+TGG 0.232965 3:+33076783 None:intergenic
CCTAATGCTGATATTGAATA+TGG 0.285356 3:-33076676 MsG0380013219.01.T01:CDS
AGCAGGGAGCTGTCCGTCTC+AGG 0.357261 3:-33076901 MsG0380013219.01.T01:CDS
TTATGTTAAGCTTGCTACTT+TGG 0.363249 3:-33077177 MsG0380013219.01.T01:CDS
AGATTGTAGTTGTATCCCTT+GGG 0.363389 3:-33076832 MsG0380013219.01.T01:CDS
CTGCTTCAACGTTCTTCGTC+TGG 0.376847 3:+33076919 None:intergenic
GAGATTGTAGTTGTATCCCT+TGG 0.382260 3:-33076833 MsG0380013219.01.T01:CDS
CACTTCCCTTCTTGATGCCT+CGG 0.398451 3:+33076719 None:intergenic
CCATATTCAATATCAGCATT+AGG 0.437256 3:+33076676 None:intergenic
CAAGTCAAATATTGAACAAA+AGG 0.441013 3:+33076875 None:intergenic
CAGACGAAGAACGTTGAAGC+AGG 0.486278 3:-33076918 MsG0380013219.01.T01:CDS
CGAGGCATCAAGAAGGGAAG+TGG 0.505162 3:-33076718 MsG0380013219.01.T01:CDS
CTCATGCCGAGGCATCAAGA+AGG 0.570754 3:-33076725 MsG0380013219.01.T01:CDS
GATTGTAGTTGTATCCCTTG+GGG 0.572574 3:-33076831 MsG0380013219.01.T01:CDS
GCAGGGAGCTGTCCGTCTCA+GGG 0.576016 3:-33076900 MsG0380013219.01.T01:CDS
TCATGCCGAGGCATCAAGAA+GGG 0.594673 3:-33076724 MsG0380013219.01.T01:CDS
ATGCTGATATTGAATATGGA+TGG 0.600385 3:-33076672 MsG0380013219.01.T01:CDS
ACAAATATACATACAGAATG+TGG 0.609862 3:+33077139 None:intergenic
ATATTTGACTTGACACCATG+CGG 0.621402 3:-33076864 MsG0380013219.01.T01:CDS
CGTTCTGCTTCATCTACAGA+GGG 0.621559 3:+33076644 None:intergenic
GAACAAAAGGAACCCTGAGA+CGG 0.632996 3:+33076888 None:intergenic
AGACGAAGAACGTTGAAGCA+GGG 0.637674 3:-33076917 MsG0380013219.01.T01:CDS
GAGGCATCAAGAAGGGAAGT+GGG 0.644826 3:-33076717 MsG0380013219.01.T01:CDS
CAATCTCTGAGATTGCCGCA+TGG 0.655920 3:+33076849 None:intergenic
ACGTTCTGCTTCATCTACAG+AGG 0.657683 3:+33076643 None:intergenic
ATTGTAGTTGTATCCCTTGG+GGG 0.670258 3:-33076830 MsG0380013219.01.T01:CDS
ACATAATATGAAGATGATCG+AGG 0.692790 3:-33076769 MsG0380013219.01.T01:CDS
CTTATGCAAAACTCATGCCG+AGG 0.694126 3:-33076736 MsG0380013219.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATGTATATTTGTTTAACAT+GGG - Chr3:33076706-33076725 MsG0380013219.01.T01:CDS 15.0%
!! ATATTATGTTCAGAACTAAT+TGG + Chr3:33077055-33077074 None:intergenic 20.0%
!! ATCGATATATATTATGTGTT+AGG - Chr3:33076741-33076760 MsG0380013219.01.T01:CDS 20.0%
!! ATGTATATATTACGAAAGTT+TGG + Chr3:33076841-33076860 None:intergenic 20.0%
!! GTATGTATATTTGTTTAACA+TGG - Chr3:33076705-33076724 MsG0380013219.01.T01:CDS 20.0%
! ACAAATATACATACAGAATG+TGG + Chr3:33076699-33076718 None:intergenic 25.0%
! ACACATAATATATATCGATG+AGG + Chr3:33076740-33076759 None:intergenic 25.0%
! CAAGTCAAATATTGAACAAA+AGG + Chr3:33076963-33076982 None:intergenic 25.0%
ACATAATATGAAGATGATCG+AGG - Chr3:33077066-33077085 MsG0380013219.01.T01:intron 30.0%
ATGCTGATATTGAATATGGA+TGG - Chr3:33077163-33077182 MsG0380013219.01.T01:CDS 30.0%
CCATATTCAATATCAGCATT+AGG + Chr3:33077162-33077181 None:intergenic 30.0%
CCTAATGCTGATATTGAATA+TGG - Chr3:33077159-33077178 MsG0380013219.01.T01:CDS 30.0%
TACATTATTCACACGTGTAT+CGG - Chr3:33076856-33076875 MsG0380013219.01.T01:CDS 30.0%
!! TTATGTTAAGCTTGCTACTT+TGG - Chr3:33076658-33076677 MsG0380013219.01.T01:CDS 30.0%
AATGGCAAAGGCAGTTAAAT+TGG + Chr3:33076884-33076903 None:intergenic 35.0%
AGATTGTAGTTGTATCCCTT+GGG - Chr3:33077003-33077022 MsG0380013219.01.T01:intron 35.0%
! GCATGAGTTTTGCATAAGTT+AGG + Chr3:33077097-33077116 None:intergenic 35.0%
!! ATATTTGACTTGACACCATG+CGG - Chr3:33076971-33076990 MsG0380013219.01.T01:intron 35.0%
ACGATTACTGCAAATGGCAA+AGG + Chr3:33076896-33076915 None:intergenic 40.0%
ATTGTAGTTGTATCCCTTGG+GGG - Chr3:33077005-33077024 MsG0380013219.01.T01:intron 40.0%
GAGATTGTAGTTGTATCCCT+TGG - Chr3:33077002-33077021 MsG0380013219.01.T01:intron 40.0%
GATTGTAGTTGTATCCCTTG+GGG - Chr3:33077004-33077023 MsG0380013219.01.T01:intron 40.0%
TACCAAAAAATAGCCCCCAA+GGG + Chr3:33077021-33077040 None:intergenic 40.0%
AGACGAAGAACGTTGAAGCA+GGG - Chr3:33076918-33076937 MsG0380013219.01.T01:CDS 45.0%
CTTATGCAAAACTCATGCCG+AGG - Chr3:33077099-33077118 MsG0380013219.01.T01:intron 45.0%
GAACAAAAGGAACCCTGAGA+CGG + Chr3:33076950-33076969 None:intergenic 45.0%
GTACCAAAAAATAGCCCCCA+AGG + Chr3:33077022-33077041 None:intergenic 45.0%
GTCTGGACGATTACTGCAAA+TGG + Chr3:33076902-33076921 None:intergenic 45.0%
!!! ATCCCTTGGGGGCTATTTTT+TGG - Chr3:33077016-33077035 MsG0380013219.01.T01:intron 45.0%
CACTTCCCTTCTTGATGCCT+CGG + Chr3:33077119-33077138 None:intergenic 50.0%
CAGACGAAGAACGTTGAAGC+AGG - Chr3:33076917-33076936 MsG0380013219.01.T01:CDS 50.0%
CTGCTTCAACGTTCTTCGTC+TGG + Chr3:33076919-33076938 None:intergenic 50.0%
! GAGGCATCAAGAAGGGAAGT+GGG - Chr3:33077118-33077137 MsG0380013219.01.T01:intron 50.0%
! TCATGCCGAGGCATCAAGAA+GGG - Chr3:33077111-33077130 MsG0380013219.01.T01:intron 50.0%
!! CAATCTCTGAGATTGCCGCA+TGG + Chr3:33076989-33077008 None:intergenic 50.0%
! CGAGGCATCAAGAAGGGAAG+TGG - Chr3:33077117-33077136 MsG0380013219.01.T01:intron 55.0%
! CTCATGCCGAGGCATCAAGA+AGG - Chr3:33077110-33077129 MsG0380013219.01.T01:intron 55.0%
AGCAGGGAGCTGTCCGTCTC+AGG - Chr3:33076934-33076953 MsG0380013219.01.T01:intron 65.0%
GCAGGGAGCTGTCCGTCTCA+GGG - Chr3:33076935-33076954 MsG0380013219.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 33076653 33077204 33076653 ID=MsG0380013219.01;Name=MsG0380013219.01
Chr3 mRNA 33076653 33077204 33076653 ID=MsG0380013219.01.T01;Parent=MsG0380013219.01;Name=MsG0380013219.01.T01;_AED=0.50;_eAED=0.52;_QI=0|0|0|1|0|0|2|0|115
Chr3 exon 33077153 33077204 33077153 ID=MsG0380013219.01.T01:exon:6338;Parent=MsG0380013219.01.T01
Chr3 exon 33076653 33076948 33076653 ID=MsG0380013219.01.T01:exon:6339;Parent=MsG0380013219.01.T01
Chr3 CDS 33077153 33077204 33077153 ID=MsG0380013219.01.T01:cds;Parent=MsG0380013219.01.T01
Chr3 CDS 33076653 33076948 33076653 ID=MsG0380013219.01.T01:cds;Parent=MsG0380013219.01.T01
Chr3 mRNA 33076653 33076907 33076653 ID=MsG0380013219.01.T02;Parent=MsG0380013219.01;Name=MsG0380013219.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|84
Chr3 exon 33076653 33076907 33076653 ID=MsG0380013219.01.T02:exon:6340;Parent=MsG0380013219.01.T02
Chr3 CDS 33076653 33076907 33076653 ID=MsG0380013219.01.T02:cds;Parent=MsG0380013219.01.T02
Chr3 mRNA 33076653 33076892 33076653 ID=MsG0380013219.01.T03;Parent=MsG0380013219.01;Name=MsG0380013219.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|79
Chr3 exon 33076653 33076892 33076653 ID=MsG0380013219.01.T03:exon:6341;Parent=MsG0380013219.01.T03
Chr3 CDS 33076653 33076892 33076653 ID=MsG0380013219.01.T03:cds;Parent=MsG0380013219.01.T03
Gene Sequence

>MsG0380013219.01.T02

TCTCAGGGTTCCTTTTGTTCAATATTTGACTTGACACCATGCGGCAATCTCAGAGATTGTAGTTGTATCCCTTGGGGGCTATTTTTTGGTACTTGCACAAATCCAATTAGTTCTGAACATAATATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCCGAGGCATCAAGAAGGGAAGTGGGAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTTTTTCCCTCTGTAGATGA

>MsG0380013219.01.T01

ATGACTTATGTTAAGCTTGCTACTTTGGCTGTCTTCATGCTCACCACATTCTTAATCGTCCAGACGAAGAACGTTGAAGCAGGGAGCTGTCCGTCTCAGGGTTCCTTTTGTTCAATATTTGACTTGACACCATGCGGCAATCTCAGAGATTGTAGTTGTATCCCTTGGGGGCTATTTTTTGGTACTTGCACAAATCCAATTAGTTCTGAACATAATATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCCGAGGCATCAAGAAGGGAAGTGGGAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTTTTTCCCTCTGTAGATGA

>MsG0380013219.01.T03

TGTTCAATATTTGACTTGACACCATGCGGCAATCTCAGAGATTGTAGTTGTATCCCTTGGGGGCTATTTTTTGGTACTTGCACAAATCCAATTAGTTCTGAACATAATATGAAGATGATCGAGGAACATCCTAACTTATGCAAAACTCATGCCGAGGCATCAAGAAGGGAAGTGGGAACTTTTGTGCTCGTTATCCTAATGCTGATATTGAATATGGATGGTTTTTCCCTCTGTAGATGA

Protein sequence

>MsG0380013219.01.T02

SQGSFCSIFDLTPCGNLRDCSCIPWGLFFGTCTNPISSEHNMKMIEEHPNLCKTHAEASRREVGTFVLVILMLILNMDGFSLCR*

>MsG0380013219.01.T01

MTYVKLATLAVFMLTTFLIVQTKNVEAGSCPSQGSFCSIFDLTPCGNLRDCSCIPWGLFFGTCTNPISSEHNMKMIEEHPNLCKTHAEASRREVGTFVLVILMLILNMDGFSLCR*

>MsG0380013219.01.T03

CSIFDLTPCGNLRDCSCIPWGLFFGTCTNPISSEHNMKMIEEHPNLCKTHAEASRREVGTFVLVILMLILNMDGFSLCR*