AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043530.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043530.01.T01 MTR_8g037800 95.604 91 3 1 1 90 1 91 3.45e-49 150
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043530.01.T01 AT2G16385 45.055 91 39 4 1 90 3 83 5.58e-18 71.6
MsG0880043530.01.T01 AT4G34600 39.506 81 38 3 11 90 13 83 1.68e-12 57.8

Find 21 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCTTCCTCCTCATTTC+TGG 0.032595 8:-26216125 MsG0880043530.01.T01:CDS
GCATCCATGAGAGGCTTCTT+AGG 0.213059 8:-26214932 MsG0880043530.01.T01:CDS
ACATGTCTATATAATATATA+TGG 0.312935 8:+26214719 None:intergenic
AGGGCTAATACAAAAGATTA+TGG 0.330875 8:-26214912 MsG0880043530.01.T01:CDS
TTTCTCTTTGCAATATACAT+AGG 0.454267 8:-26216233 None:intergenic
CAATTTGGTATGAGCTTGAA+AGG 0.467961 8:+26214855 None:intergenic
AAAATGAATCTTGATATAGT+TGG 0.476392 8:-26214819 MsG0880043530.01.T01:three_prime_UTR
CATCCATGAGAGGCTTCTTA+GGG 0.497324 8:-26214931 MsG0880043530.01.T01:CDS
CACTAAGATAATTAAACAGC+GGG 0.498673 8:+26214754 None:intergenic
ATCTTGATATAGTTGGAAGT+TGG 0.508490 8:-26214812 MsG0880043530.01.T01:three_prime_UTR
TAGCCCTAAGAAGCCTCTCA+TGG 0.540665 8:+26214928 None:intergenic
CCATGTTGAGTAGTGCTAGT+TGG 0.554512 8:+26216174 None:intergenic
CCAACTAGCACTACTCAACA+TGG 0.556949 8:-26216174 MsG0880043530.01.T01:exon
AGCAAAGAAGTCAATGAATG+AGG 0.570640 8:-26214967 MsG0880043530.01.T01:CDS
AAGTAAGAAGCATCCATGAG+AGG 0.614358 8:-26214941 MsG0880043530.01.T01:CDS
TGTCTATATAATATATATGG+AGG 0.617095 8:+26214722 None:intergenic
TCAAAAGTGAGCCAGAAATG+AGG 0.623763 8:+26216114 None:intergenic
TTTGGTATGAGCTTGAAAGG+AGG 0.661865 8:+26214858 None:intergenic
AAAGTGAGCCAGAAATGAGG+AGG 0.663281 8:+26216117 None:intergenic
ACACTAAGATAATTAAACAG+CGG 0.681926 8:+26214753 None:intergenic
AGATGTGAATGCAGTCCATG+AGG 0.750934 8:-26215693 MsG0880043530.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTTTTTAAAAATGAATT+AGG + Chr8:26215695-26215714 None:intergenic 10.0%
!!! TACTTTCTTTTTTTAAATTA+AGG + Chr8:26215727-26215746 None:intergenic 10.0%
!! ACATGTCTATATAATATATA+TGG + Chr8:26216121-26216140 None:intergenic 15.0%
!! TGAAAATAAACGTAAAAAAT+TGG + Chr8:26215802-26215821 None:intergenic 15.0%
!! AAAATGAATCTTGATATAGT+TGG - Chr8:26216018-26216037 MsG0880043530.01.T01:intron 20.0%
!! ATACTGTAATAACTTAAATG+TGG + Chr8:26215347-26215366 None:intergenic 20.0%
!! TAAACCACAAATATTAAACA+AGG + Chr8:26215440-26215459 None:intergenic 20.0%
!! TGTCTATATAATATATATGG+AGG + Chr8:26216118-26216137 None:intergenic 20.0%
!!! TTAAGAGTGTGTAATTATAA+TGG - Chr8:26214892-26214911 MsG0880043530.01.T01:CDS 20.0%
!!! TTTTCCTTGTTTAATATTTG+TGG - Chr8:26215433-26215452 MsG0880043530.01.T01:intron 20.0%
!!! TTTTTAACGTTCATTCAATT+TGG + Chr8:26216000-26216019 None:intergenic 20.0%
! AAGATATTTATTTGCGAGTT+AGG - Chr8:26215563-26215582 MsG0880043530.01.T01:intron 25.0%
! ACACTAAGATAATTAAACAG+CGG + Chr8:26216087-26216106 None:intergenic 25.0%
!!! GAGTGCTTTATTTTTGATAT+AGG - Chr8:26215843-26215862 MsG0880043530.01.T01:intron 25.0%
!!! GCAGGTTAGTTTTTTTTTTA+AGG - Chr8:26214754-26214773 MsG0880043530.01.T01:three_prime_UTR 25.0%
AGGGCTAATACAAAAGATTA+TGG - Chr8:26215925-26215944 MsG0880043530.01.T01:intron 30.0%
ATCTTGATATAGTTGGAAGT+TGG - Chr8:26216025-26216044 MsG0880043530.01.T01:intron 30.0%
CACTAAGATAATTAAACAGC+GGG + Chr8:26216086-26216105 None:intergenic 30.0%
CTCACTTGAAAACAAAGAAA+TGG - Chr8:26215464-26215483 MsG0880043530.01.T01:intron 30.0%
GTAGAGTTTGTCTTGTATAT+GGG - Chr8:26215486-26215505 MsG0880043530.01.T01:intron 30.0%
TATAGTATTAAGCACACACT+AGG + Chr8:26215085-26215104 None:intergenic 30.0%
TATCTGACACCATAAGATAA+AGG + Chr8:26214791-26214810 None:intergenic 30.0%
! TTTACAAGTTGAGTGTTAGA+TGG + Chr8:26215757-26215776 None:intergenic 30.0%
!! GAAAGTACTCATGAAATAGA+AGG - Chr8:26215382-26215401 MsG0880043530.01.T01:intron 30.0%
AAAGGAGGCTTAGAAAAAGT+TGG + Chr8:26215967-26215986 None:intergenic 35.0%
AATTGGAAACCATGCTCTTT+AGG + Chr8:26215785-26215804 None:intergenic 35.0%
AGCAAAGAAGTCAATGAATG+AGG - Chr8:26215870-26215889 MsG0880043530.01.T01:intron 35.0%
CAATTTGGTATGAGCTTGAA+AGG + Chr8:26215985-26216004 None:intergenic 35.0%
GGTAGAGTTTGTCTTGTATA+TGG - Chr8:26215485-26215504 MsG0880043530.01.T01:intron 35.0%
GTGTCAGATACATGTTCTTA+TGG - Chr8:26214801-26214820 MsG0880043530.01.T01:three_prime_UTR 35.0%
GTGTGCTTAATACTATATGC+AGG - Chr8:26215088-26215107 MsG0880043530.01.T01:intron 35.0%
TAAACAAAGCCTAAAGAGCA+TGG - Chr8:26215773-26215792 MsG0880043530.01.T01:intron 35.0%
TTCTTGCAGCCTTTATCTTA+TGG - Chr8:26214779-26214798 MsG0880043530.01.T01:three_prime_UTR 35.0%
! ACCATTGATTGTTAGGTACA+AGG + Chr8:26215307-26215326 None:intergenic 35.0%
! TATTCCATGATCTTCTGAAG+TGG - Chr8:26214936-26214955 MsG0880043530.01.T01:CDS 35.0%
!! ATTCCATGATCTTCTGAAGT+GGG - Chr8:26214937-26214956 MsG0880043530.01.T01:CDS 35.0%
!! GTTTTGCACCATTGATTGTT+AGG + Chr8:26215314-26215333 None:intergenic 35.0%
!! TCACTTTTGACAACTTTTGC+AGG - Chr8:26214736-26214755 MsG0880043530.01.T01:three_prime_UTR 35.0%
ATTTGCGAGTTAGGTTTACG+AGG - Chr8:26215572-26215591 MsG0880043530.01.T01:intron 40.0%
GAAGTGAATTTCCTACCTCA+TGG + Chr8:26215162-26215181 None:intergenic 40.0%
GCCTTGTACCTAACAATCAA+TGG - Chr8:26215303-26215322 MsG0880043530.01.T01:intron 40.0%
TCAAAAGTGAGCCAGAAATG+AGG + Chr8:26214726-26214745 None:intergenic 40.0%
TTTGCGAGTTAGGTTTACGA+GGG - Chr8:26215573-26215592 MsG0880043530.01.T01:intron 40.0%
TTTGGTATGAGCTTGAAAGG+AGG + Chr8:26215982-26216001 None:intergenic 40.0%
! CAAGTTGAGTGTTAGATGGA+AGG + Chr8:26215753-26215772 None:intergenic 40.0%
!! AAGTAAGAAGCATCCATGAG+AGG - Chr8:26215896-26215915 MsG0880043530.01.T01:intron 40.0%
!! GTACAAGGCTCTAAACATGA+TGG + Chr8:26215292-26215311 None:intergenic 40.0%
AAAGTGAGCCAGAAATGAGG+AGG + Chr8:26214723-26214742 None:intergenic 45.0%
AGATGTGAATGCAGTCCATG+AGG - Chr8:26215144-26215163 MsG0880043530.01.T01:intron 45.0%
CATCCATGAGAGGCTTCTTA+GGG - Chr8:26215906-26215925 MsG0880043530.01.T01:intron 45.0%
CCAACTAGCACTACTCAACA+TGG - Chr8:26214663-26214682 MsG0880043530.01.T01:three_prime_UTR 45.0%
CCATGTTGAGTAGTGCTAGT+TGG + Chr8:26214666-26214685 None:intergenic 45.0%
CTTCCCACTTCAGAAGATCA+TGG + Chr8:26214943-26214962 None:intergenic 45.0%
CTTCTCTTCCTCCTCATTTC+TGG - Chr8:26214712-26214731 MsG0880043530.01.T01:three_prime_UTR 45.0%
GGCTTAGAAAAAGTTGGCGA+TGG + Chr8:26215961-26215980 None:intergenic 45.0%
!!! AGGTTTACGAGGGAGTGTTT+TGG - Chr8:26215583-26215602 MsG0880043530.01.T01:intron 45.0%
GCATCCATGAGAGGCTTCTT+AGG - Chr8:26215905-26215924 MsG0880043530.01.T01:intron 50.0%
GTGAATGCAGTCCATGAGGT+AGG - Chr8:26215148-26215167 MsG0880043530.01.T01:intron 50.0%
TAGCCCTAAGAAGCCTCTCA+TGG + Chr8:26215912-26215931 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 26214611 26216248 26214611 ID=MsG0880043530.01;Name=MsG0880043530.01
Chr8 mRNA 26214611 26216248 26214611 ID=MsG0880043530.01.T01;Parent=MsG0880043530.01;Name=MsG0880043530.01.T01;_AED=0.50;_eAED=0.50;_QI=71|1|1|1|0|0|3|243|90
Chr8 exon 26214611 26214994 26214611 ID=MsG0880043530.01.T01:exon:5225;Parent=MsG0880043530.01.T01
Chr8 exon 26215694 26215749 26215694 ID=MsG0880043530.01.T01:exon:5224;Parent=MsG0880043530.01.T01
Chr8 exon 26216102 26216248 26216102 ID=MsG0880043530.01.T01:exon:5223;Parent=MsG0880043530.01.T01
Chr8 five_prime_UTR 26216178 26216248 26216178 ID=MsG0880043530.01.T01:five_prime_utr;Parent=MsG0880043530.01.T01
Chr8 CDS 26216102 26216177 26216102 ID=MsG0880043530.01.T01:cds;Parent=MsG0880043530.01.T01
Chr8 CDS 26215694 26215749 26215694 ID=MsG0880043530.01.T01:cds;Parent=MsG0880043530.01.T01
Chr8 CDS 26214854 26214994 26214854 ID=MsG0880043530.01.T01:cds;Parent=MsG0880043530.01.T01
Chr8 three_prime_UTR 26214611 26214853 26214611 ID=MsG0880043530.01.T01:three_prime_utr;Parent=MsG0880043530.01.T01
Gene Sequence

>MsG0880043530.01.T01

ATGGATTTCATGTTTCTCAAGAAATTCACACTTCTCTTCCTCCTCATTTCTGGCTCACTTTTGACAACTTTTGCAGGTCGAGCATCAAATTTCATCAGAATCTCAAATGAAGATGTGAATGCAGTCCATGAGGTAACAGCAAAGAAGTCAATGAATGAGGAAGAAGTAAGAAGCATCCATGAGAGGCTTCTTAGGGCTAATACAAAAGATTATGGACGATATGATCCATCGCCAACTTTTTCTAAGCCTCCTTTCAAGCTCATACCAAATTGA

Protein sequence

>MsG0880043530.01.T01

MDFMFLKKFTLLFLLISGSLLTTFAGRASNFIRISNEDVNAVHEVTAKKSMNEEEVRSIHERLLRANTKDYGRYDPSPTFSKPPFKLIPN*