AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020923.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020923.01.T01 MTR_6g472230 42.051 195 110 3 45 237 1 194 4.11e-46 165
MsG0480020923.01.T01 MTR_3g093120 35.743 249 150 6 5 251 287 527 7.11e-45 158
MsG0480020923.01.T01 MTR_3g022740 33.659 205 87 4 38 242 5 160 6.19e-30 118
MsG0480020923.01.T01 MTR_3g448730 33.607 122 81 0 130 251 101 222 1.98e-26 103
MsG0480020923.01.T01 MTR_3g452820 32.222 180 83 4 37 216 21 161 2.50e-23 94.0
MsG0480020923.01.T01 MTR_8g059225 35.577 104 67 0 112 215 71 174 6.35e-23 92.4
MsG0480020923.01.T01 MTR_7g026095 36.641 131 81 2 41 170 30 159 1.20e-20 89.7
MsG0480020923.01.T01 MTR_6g033925 35.915 142 86 3 13 153 1 138 4.62e-20 85.9
MsG0480020923.01.T01 MTR_2g075320 28.505 214 121 5 41 253 105 287 7.65e-19 84.3
MsG0480020923.01.T01 MTR_6g060522 34.746 118 76 1 138 254 3 120 7.77e-18 79.3
MsG0480020923.01.T01 MTR_7g056567 34.234 111 72 1 144 253 24 134 9.23e-17 75.9
MsG0480020923.01.T01 MTR_8g011410 30.337 178 101 4 67 242 815 971 3.78e-16 78.2
MsG0480020923.01.T01 MTR_2g460640 36.111 108 62 3 11 117 1 102 2.20e-15 71.6
MsG0480020923.01.T01 MTR_1g051400 35.556 90 57 1 81 169 73 162 1.13e-14 70.5
MsG0480020923.01.T01 MTR_7g056447 37.037 81 51 0 173 253 212 292 1.34e-14 72.4
MsG0480020923.01.T01 MTR_2g017850 21.608 199 110 3 58 254 187 341 4.66e-12 65.5
MsG0480020923.01.T01 MTR_0303s0010 41.791 67 39 0 96 162 98 164 2.06e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020923.01.T01 AT4G23160 36.667 210 124 4 4 211 351 553 1.40e-31 123
MsG0480020923.01.T01 AT4G23160 36.667 210 124 4 4 211 351 553 1.40e-31 123

Find 70 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGATTGGTTAGTCGGTTTA+TGG 0.209777 4:+50623313 MsG0480020923.01.T01:CDS
ACAGGAAGTGGGGTAGAAAT+TGG 0.289482 4:-50623152 None:intergenic
TATTTGGATGCAAAGGTTAA+TGG 0.314202 4:+50623616 MsG0480020923.01.T01:CDS
GGTTATGTGTTCACGCTTGC+TGG 0.317322 4:+50623497 MsG0480020923.01.T01:CDS
AAGCGTGTAAGGAAGCTATT+TGG 0.329376 4:+50623600 MsG0480020923.01.T01:CDS
AGAATCATGAGGTACATTAA+AGG 0.348284 4:+50623371 MsG0480020923.01.T01:CDS
TAGTCGGTTTATGGCGGATC+CGG 0.358965 4:+50623322 MsG0480020923.01.T01:CDS
TCGATCCTCATATGGCCTAT+TGG 0.390032 4:+50623871 MsG0480020923.01.T01:CDS
TCAGGTGTTGCAATATGTTT+CGG 0.402671 4:+50623398 MsG0480020923.01.T01:CDS
CCCACTGCTTGTGCAATGTC+TGG 0.403345 4:-50623293 None:intergenic
ATCCTCTCCGCTTCGTTGCT+AGG 0.409917 4:-50623203 None:intergenic
TACATCTGCTAGGTTATCAT+TGG 0.424692 4:-50623810 None:intergenic
TTTGTTCTTGAATGAAAAGC+TGG 0.436926 4:-50623713 None:intergenic
AAATAGCTTCCTTACACGCT+TGG 0.437349 4:-50623598 None:intergenic
TCAGTCGCTACGTTTCCAAT+AGG 0.437994 4:-50623886 None:intergenic
TGTTCGAGAAGTAGTAGAGG+AGG 0.444479 4:+50623760 MsG0480020923.01.T01:CDS
GGCAGCTACCCAAGCGTGTA+AGG 0.445104 4:+50623589 MsG0480020923.01.T01:CDS
AGCTACAACTGGTTGTAACT+TGG 0.445412 4:-50623540 None:intergenic
AATTTAAAATTCACAGGAAG+TGG 0.448026 4:-50623164 None:intergenic
GTATGGTGTCGATCCTCATA+TGG 0.454773 4:+50623863 MsG0480020923.01.T01:CDS
GTCGTTGACAGAGCTACAAC+TGG 0.468469 4:-50623551 None:intergenic
AATAGCTTCCTTACACGCTT+GGG 0.469268 4:-50623597 None:intergenic
GTTCGAGAAGTAGTAGAGGA+GGG 0.470336 4:+50623761 MsG0480020923.01.T01:CDS
AGTAGTAGAGGAGGGGAGTG+TGG 0.471646 4:+50623769 MsG0480020923.01.T01:CDS
CATTGCACAAGCAGTGGGAT+TGG 0.476054 4:+50623298 MsG0480020923.01.T01:CDS
TACATATCATGGCATACATA+AGG 0.477860 4:-50623265 None:intergenic
AGTCGGTTTATGGCGGATCC+GGG 0.479418 4:+50623323 MsG0480020923.01.T01:CDS
AAGCAGTGGGATTGGTTAGT+CGG 0.481735 4:+50623306 MsG0480020923.01.T01:CDS
CGTTTCCAATAGGCCATATG+AGG 0.487317 4:-50623876 None:intergenic
TTCGAGAAGTAGTAGAGGAG+GGG 0.490811 4:+50623762 MsG0480020923.01.T01:CDS
GTCTGGTCTTGTACATATCA+TGG 0.492315 4:-50623276 None:intergenic
GATAAAAGAAAATCTACTAC+TGG 0.492564 4:+50623476 MsG0480020923.01.T01:CDS
GAGGATAATTTAAAATTCAC+AGG 0.497866 4:-50623170 None:intergenic
CATTCAAGAACAAAATACAT+AGG 0.502125 4:+50623722 MsG0480020923.01.T01:CDS
GTCTCGAGTACCGTATGAAT+CGG 0.503843 4:+50623232 MsG0480020923.01.T01:CDS
CAAAGGTTAATGGAGGAACT+CGG 0.514212 4:+50623626 MsG0480020923.01.T01:CDS
GAGGATCAGAGTTAACTGTC+AGG 0.516487 4:+50623420 MsG0480020923.01.T01:CDS
CGGATCCGGGTAAAGAGCAT+TGG 0.525904 4:+50623336 MsG0480020923.01.T01:CDS
TTGCTGGAGGAGCAGTAAGT+TGG 0.528057 4:+50623513 MsG0480020923.01.T01:CDS
AGGTACATTAAAGGAACCTC+AGG 0.532112 4:+50623380 MsG0480020923.01.T01:CDS
ATTTAAAATTCACAGGAAGT+GGG 0.535808 4:-50623163 None:intergenic
TCAACGCTGACAAGTTTGTA+TGG 0.540945 4:+50623846 MsG0480020923.01.T01:CDS
AACGACAGAAGCTGAGTACA+TGG 0.553131 4:+50623568 MsG0480020923.01.T01:CDS
TAGCAACGAAGCGGAGAGGA+TGG 0.554421 4:+50623205 MsG0480020923.01.T01:CDS
CTTCCCACCGCCGATTCATA+CGG 0.557007 4:-50623242 None:intergenic
ACCAGACATTGCACAAGCAG+TGG 0.557126 4:+50623292 MsG0480020923.01.T01:CDS
AGGAAGCTATTTGGATGCAA+AGG 0.561391 4:+50623609 MsG0480020923.01.T01:CDS
GCATTCCAATGCTCTTTACC+CGG 0.576147 4:-50623341 None:intergenic
ACTTTGTCATTACATCTGCT+AGG 0.578060 4:-50623820 None:intergenic
TTTAAAATTCACAGGAAGTG+GGG 0.578255 4:-50623162 None:intergenic
AGGATCAGAGTTAACTGTCA+GGG 0.582959 4:+50623421 MsG0480020923.01.T01:CDS
GGAGGAACTCGGGCACAAGC+AGG 0.583526 4:+50623637 MsG0480020923.01.T01:CDS
AGAGTGCCTTGCATATTGCA+AGG 0.594651 4:+50623687 MsG0480020923.01.T01:CDS
AGTACCGTATGAATCGGCGG+TGG 0.599601 4:+50623238 MsG0480020923.01.T01:CDS
ATTGGTTAGTCGGTTTATGG+CGG 0.603784 4:+50623316 MsG0480020923.01.T01:CDS
CCAGACATTGCACAAGCAGT+GGG 0.614822 4:+50623293 MsG0480020923.01.T01:CDS
TTGGATGCAAAGGTTAATGG+AGG 0.617483 4:+50623619 MsG0480020923.01.T01:CDS
AAAGGTTAATGGAGGAACTC+GGG 0.623045 4:+50623627 MsG0480020923.01.T01:CDS
TGGATCCCTTGCAATATGCA+AGG 0.625026 4:-50623693 None:intergenic
GAGTGCCTTGCATATTGCAA+GGG 0.638056 4:+50623688 MsG0480020923.01.T01:CDS
GTTGCTAGGACTCATACTTG+AGG 0.638288 4:-50623189 None:intergenic
GTACCGTATGAATCGGCGGT+GGG 0.638767 4:+50623239 MsG0480020923.01.T01:CDS
GGTGTTGCAATATGTTTCGG+AGG 0.640818 4:+50623401 MsG0480020923.01.T01:CDS
TATGTGTTCACGCTTGCTGG+AGG 0.654870 4:+50623500 MsG0480020923.01.T01:CDS
TATGAGTCCTAGCAACGAAG+CGG 0.667310 4:+50623196 MsG0480020923.01.T01:CDS
GGATCAGAGTTAACTGTCAG+GGG 0.668527 4:+50623422 MsG0480020923.01.T01:CDS
GTCCTAGCAACGAAGCGGAG+AGG 0.675999 4:+50623201 MsG0480020923.01.T01:CDS
TCGAGTACCGTATGAATCGG+CGG 0.678195 4:+50623235 MsG0480020923.01.T01:CDS
GAAACATATTGCAACACCTG+AGG 0.695845 4:-50623396 None:intergenic
ATGCTGTTAAAAGAATCATG+AGG 0.723101 4:+50623360 MsG0480020923.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CATTCAAGAACAAAATACAT+AGG + Chr4:50623722-50623741 MsG0480020923.01.T01:CDS 25.0%
! GATAAAAGAAAATCTACTAC+TGG + Chr4:50623476-50623495 MsG0480020923.01.T01:CDS 25.0%
!! AATTTAAAATTCACAGGAAG+TGG - Chr4:50623167-50623186 None:intergenic 25.0%
!! ATTTAAAATTCACAGGAAGT+GGG - Chr4:50623166-50623185 None:intergenic 25.0%
!! GAGGATAATTTAAAATTCAC+AGG - Chr4:50623173-50623192 None:intergenic 25.0%
AGAATCATGAGGTACATTAA+AGG + Chr4:50623371-50623390 MsG0480020923.01.T01:CDS 30.0%
ATGCTGTTAAAAGAATCATG+AGG + Chr4:50623360-50623379 MsG0480020923.01.T01:CDS 30.0%
TACATATCATGGCATACATA+AGG - Chr4:50623268-50623287 None:intergenic 30.0%
TATTTGGATGCAAAGGTTAA+TGG + Chr4:50623616-50623635 MsG0480020923.01.T01:CDS 30.0%
TTTGTTCTTGAATGAAAAGC+TGG - Chr4:50623716-50623735 None:intergenic 30.0%
! TTTAAAATTCACAGGAAGTG+GGG - Chr4:50623165-50623184 None:intergenic 30.0%
!!! TATGTTGATTCAGATTTTGC+AGG + Chr4:50623446-50623465 MsG0480020923.01.T01:CDS 30.0%
ACTTTGTCATTACATCTGCT+AGG - Chr4:50623823-50623842 None:intergenic 35.0%
TACATCTGCTAGGTTATCAT+TGG - Chr4:50623813-50623832 None:intergenic 35.0%
! TCAGGTGTTGCAATATGTTT+CGG + Chr4:50623398-50623417 MsG0480020923.01.T01:CDS 35.0%
! TTTTGTTCGAGAAGTAGTAG+AGG + Chr4:50623757-50623776 MsG0480020923.01.T01:CDS 35.0%
AAAGGTTAATGGAGGAACTC+GGG + Chr4:50623627-50623646 MsG0480020923.01.T01:CDS 40.0%
AAATAGCTTCCTTACACGCT+TGG - Chr4:50623601-50623620 None:intergenic 40.0%
AAGCGTGTAAGGAAGCTATT+TGG + Chr4:50623600-50623619 MsG0480020923.01.T01:CDS 40.0%
AATAGCTTCCTTACACGCTT+GGG - Chr4:50623600-50623619 None:intergenic 40.0%
AGCTACAACTGGTTGTAACT+TGG - Chr4:50623543-50623562 None:intergenic 40.0%
AGGAAGCTATTTGGATGCAA+AGG + Chr4:50623609-50623628 MsG0480020923.01.T01:CDS 40.0%
AGGATCAGAGTTAACTGTCA+GGG + Chr4:50623421-50623440 MsG0480020923.01.T01:CDS 40.0%
AGGTACATTAAAGGAACCTC+AGG + Chr4:50623380-50623399 MsG0480020923.01.T01:CDS 40.0%
CAAAGGTTAATGGAGGAACT+CGG + Chr4:50623626-50623645 MsG0480020923.01.T01:CDS 40.0%
GAAACATATTGCAACACCTG+AGG - Chr4:50623399-50623418 None:intergenic 40.0%
TCAACGCTGACAAGTTTGTA+TGG + Chr4:50623846-50623865 MsG0480020923.01.T01:CDS 40.0%
TTGGATGCAAAGGTTAATGG+AGG + Chr4:50623619-50623638 MsG0480020923.01.T01:CDS 40.0%
! ATTGGTTAGTCGGTTTATGG+CGG + Chr4:50623316-50623335 MsG0480020923.01.T01:CDS 40.0%
!! GTCTGGTCTTGTACATATCA+TGG - Chr4:50623279-50623298 None:intergenic 40.0%
AACGACAGAAGCTGAGTACA+TGG + Chr4:50623568-50623587 MsG0480020923.01.T01:CDS 45.0%
ACAGGAAGTGGGGTAGAAAT+TGG - Chr4:50623155-50623174 None:intergenic 45.0%
CGTTTCCAATAGGCCATATG+AGG - Chr4:50623879-50623898 None:intergenic 45.0%
GAGGATCAGAGTTAACTGTC+AGG + Chr4:50623420-50623439 MsG0480020923.01.T01:CDS 45.0%
GAGTGCCTTGCATATTGCAA+GGG + Chr4:50623688-50623707 MsG0480020923.01.T01:CDS 45.0%
GCATTCCAATGCTCTTTACC+CGG - Chr4:50623344-50623363 None:intergenic 45.0%
GGATCAGAGTTAACTGTCAG+GGG + Chr4:50623422-50623441 MsG0480020923.01.T01:CDS 45.0%
GTTCGAGAAGTAGTAGAGGA+GGG + Chr4:50623761-50623780 MsG0480020923.01.T01:CDS 45.0%
GTTGCTAGGACTCATACTTG+AGG - Chr4:50623192-50623211 None:intergenic 45.0%
TATGAGTCCTAGCAACGAAG+CGG + Chr4:50623196-50623215 MsG0480020923.01.T01:CDS 45.0%
TCGATCCTCATATGGCCTAT+TGG + Chr4:50623871-50623890 MsG0480020923.01.T01:CDS 45.0%
TGGATCCCTTGCAATATGCA+AGG - Chr4:50623696-50623715 None:intergenic 45.0%
TGTTCGAGAAGTAGTAGAGG+AGG + Chr4:50623760-50623779 MsG0480020923.01.T01:CDS 45.0%
TTCGAGAAGTAGTAGAGGAG+GGG + Chr4:50623762-50623781 MsG0480020923.01.T01:CDS 45.0%
! AGAGTGCCTTGCATATTGCA+AGG + Chr4:50623687-50623706 MsG0480020923.01.T01:CDS 45.0%
! GGTGTTGCAATATGTTTCGG+AGG + Chr4:50623401-50623420 MsG0480020923.01.T01:CDS 45.0%
! GTCTCGAGTACCGTATGAAT+CGG + Chr4:50623232-50623251 MsG0480020923.01.T01:CDS 45.0%
!! AAGCAGTGGGATTGGTTAGT+CGG + Chr4:50623306-50623325 MsG0480020923.01.T01:CDS 45.0%
!! GGGATTGGTTAGTCGGTTTA+TGG + Chr4:50623313-50623332 MsG0480020923.01.T01:CDS 45.0%
!! GTATGGTGTCGATCCTCATA+TGG + Chr4:50623863-50623882 MsG0480020923.01.T01:CDS 45.0%
ACCAGACATTGCACAAGCAG+TGG + Chr4:50623292-50623311 MsG0480020923.01.T01:CDS 50.0%
CATTGCACAAGCAGTGGGAT+TGG + Chr4:50623298-50623317 MsG0480020923.01.T01:CDS 50.0%
CCAGACATTGCACAAGCAGT+GGG + Chr4:50623293-50623312 MsG0480020923.01.T01:CDS 50.0%
GGTTATGTGTTCACGCTTGC+TGG + Chr4:50623497-50623516 MsG0480020923.01.T01:CDS 50.0%
GTCGTTGACAGAGCTACAAC+TGG - Chr4:50623554-50623573 None:intergenic 50.0%
TTGCTGGAGGAGCAGTAAGT+TGG + Chr4:50623513-50623532 MsG0480020923.01.T01:CDS 50.0%
! TATGTGTTCACGCTTGCTGG+AGG + Chr4:50623500-50623519 MsG0480020923.01.T01:CDS 50.0%
! TCGAGTACCGTATGAATCGG+CGG + Chr4:50623235-50623254 MsG0480020923.01.T01:CDS 50.0%
!! TAGTCGGTTTATGGCGGATC+CGG + Chr4:50623322-50623341 MsG0480020923.01.T01:CDS 50.0%
AGTACCGTATGAATCGGCGG+TGG + Chr4:50623238-50623257 MsG0480020923.01.T01:CDS 55.0%
AGTAGTAGAGGAGGGGAGTG+TGG + Chr4:50623769-50623788 MsG0480020923.01.T01:CDS 55.0%
ATCCTCTCCGCTTCGTTGCT+AGG - Chr4:50623206-50623225 None:intergenic 55.0%
CCCACTGCTTGTGCAATGTC+TGG - Chr4:50623296-50623315 None:intergenic 55.0%
GTACCGTATGAATCGGCGGT+GGG + Chr4:50623239-50623258 MsG0480020923.01.T01:CDS 55.0%
TAGCAACGAAGCGGAGAGGA+TGG + Chr4:50623205-50623224 MsG0480020923.01.T01:CDS 55.0%
! CGGATCCGGGTAAAGAGCAT+TGG + Chr4:50623336-50623355 MsG0480020923.01.T01:CDS 55.0%
!! AGTCGGTTTATGGCGGATCC+GGG + Chr4:50623323-50623342 MsG0480020923.01.T01:CDS 55.0%
!! CTTCCCACCGCCGATTCATA+CGG - Chr4:50623245-50623264 None:intergenic 55.0%
GGCAGCTACCCAAGCGTGTA+AGG + Chr4:50623589-50623608 MsG0480020923.01.T01:CDS 60.0%
GTCCTAGCAACGAAGCGGAG+AGG + Chr4:50623201-50623220 MsG0480020923.01.T01:CDS 60.0%
GGAGGAACTCGGGCACAAGC+AGG + Chr4:50623637-50623656 MsG0480020923.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 50623137 50623901 50623137 ID=MsG0480020923.01;Name=MsG0480020923.01
Chr4 mRNA 50623137 50623901 50623137 ID=MsG0480020923.01.T01;Parent=MsG0480020923.01;Name=MsG0480020923.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|254
Chr4 exon 50623137 50623901 50623137 ID=MsG0480020923.01.T01:exon:315;Parent=MsG0480020923.01.T01
Chr4 CDS 50623137 50623901 50623137 ID=MsG0480020923.01.T01:cds;Parent=MsG0480020923.01.T01
Gene Sequence

>MsG0480020923.01.T01

ATGCAAGACTGTAAGCCAATTTCTACCCCACTTCCTGTGAATTTTAAATTATCCTCAAGTATGAGTCCTAGCAACGAAGCGGAGAGGATGGAAATGTCTCGAGTACCGTATGAATCGGCGGTGGGAAGCCTTATGTATGCCATGATATGTACAAGACCAGACATTGCACAAGCAGTGGGATTGGTTAGTCGGTTTATGGCGGATCCGGGTAAAGAGCATTGGAATGCTGTTAAAAGAATCATGAGGTACATTAAAGGAACCTCAGGTGTTGCAATATGTTTCGGAGGATCAGAGTTAACTGTCAGGGGTTATGTTGATTCAGATTTTGCAGGTGATCTTGATAAAAGAAAATCTACTACTGGTTATGTGTTCACGCTTGCTGGAGGAGCAGTAAGTTGGTTGTCCAAGTTACAACCAGTTGTAGCTCTGTCAACGACAGAAGCTGAGTACATGGCAGCTACCCAAGCGTGTAAGGAAGCTATTTGGATGCAAAGGTTAATGGAGGAACTCGGGCACAAGCAGGAACAAATTACTGTGTATTGTGACAGTCAGAGTGCCTTGCATATTGCAAGGGATCCAGCTTTTCATTCAAGAACAAAATACATAGGCGTTCAATATCATTTTGTTCGAGAAGTAGTAGAGGAGGGGAGTGTGGATATGCAAAAGATTCACACCAATGATAACCTAGCAGATGTAATGACAAAGTCGATCAACGCTGACAAGTTTGTATGGTGTCGATCCTCATATGGCCTATTGGAAACGTAG

Protein sequence

>MsG0480020923.01.T01

MQDCKPISTPLPVNFKLSSSMSPSNEAERMEMSRVPYESAVGSLMYAMICTRPDIAQAVGLVSRFMADPGKEHWNAVKRIMRYIKGTSGVAICFGGSELTVRGYVDSDFAGDLDKRKSTTGYVFTLAGGAVSWLSKLQPVVALSTTEAEYMAATQACKEAIWMQRLMEELGHKQEQITVYCDSQSALHIARDPAFHSRTKYIGVQYHFVREVVEEGSVDMQKIHTNDNLADVMTKSINADKFVWCRSSYGLLET*