AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045582.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045582.01.T01 MTR_8g074530 75.000 216 3 2 1 170 95 305 7.69e-106 305
MsG0880045582.01.T01 MTR_5g023290 49.315 219 62 4 1 170 91 309 3.72e-61 192
MsG0880045582.01.T01 MTR_8g074510 36.574 216 84 4 1 169 78 287 2.13e-32 117
MsG0880045582.01.T01 MTR_8g074450 37.037 216 83 4 1 169 80 289 4.04e-31 114
MsG0880045582.01.T01 MTR_8g074420 37.500 216 82 4 1 169 80 289 1.23e-30 112
MsG0880045582.01.T01 MTR_5g023370 35.648 216 80 5 1 169 81 284 5.44e-26 100
MsG0880045582.01.T01 MTR_5g023520 33.953 215 86 3 1 169 80 284 1.10e-24 97.1
MsG0880045582.01.T01 MTR_5g023350 34.722 216 82 5 1 169 81 284 1.61e-24 96.7
MsG0880045582.01.T01 MTR_5g023390 34.259 216 83 5 1 169 81 284 3.81e-24 95.5
MsG0880045582.01.T01 MTR_5g023340 35.349 215 82 5 1 169 81 284 6.36e-24 95.1
MsG0880045582.01.T01 MTR_5g023580 34.112 214 86 4 1 167 81 286 2.29e-22 92.0
MsG0880045582.01.T01 MTR_5g023550 33.796 216 84 5 1 169 80 283 2.50e-22 90.9
MsG0880045582.01.T01 MTR_5g023320 34.419 215 81 5 1 169 82 282 3.82e-22 90.5
MsG0880045582.01.T01 MTR_5g023600 34.259 216 83 5 1 169 81 284 4.97e-22 90.1
MsG0880045582.01.T01 MTR_5g023530 31.944 216 89 4 1 169 81 285 4.31e-20 84.7
MsG0880045582.01.T01 MTR_5g023470 31.481 216 90 4 1 169 81 285 1.66e-19 83.2
MsG0880045582.01.T01 MTR_4g070530 31.193 218 92 7 1 169 87 295 1.92e-16 75.1
MsG0880045582.01.T01 MTR_5g023460 32.487 197 70 5 20 169 11 191 3.49e-16 72.8
MsG0880045582.01.T01 MTR_4g070590 31.628 215 97 6 1 169 90 300 1.26e-15 72.8
MsG0880045582.01.T01 MTR_1g037820 31.667 180 104 5 9 169 46 225 1.15e-14 69.3
MsG0880045582.01.T01 MTR_4g070540 28.638 213 100 5 1 167 87 293 1.98e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045582.01.T01 AT4G35210 29.592 196 91 6 1 167 73 250 3.13e-13 65.9
MsG0880045582.01.T01 AT4G35710 29.954 217 89 6 1 169 81 282 5.12e-13 65.5
MsG0880045582.01.T01 AT4G35200 30.151 199 89 6 1 167 73 253 2.15e-12 63.5

Find 21 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTAGTAGTTTGATGATTA+TGG 0.216552 8:+61329934 MsG0880045582.01.T01:CDS
GCTATTGAAGGAATTGAAAA+TGG 0.219192 8:+61330028 MsG0880045582.01.T01:CDS
TGAGATCAAAGGTTAATAAA+TGG 0.320655 8:+61329822 MsG0880045582.01.T01:intron
ACATGGAAGGTTAGAAGCTT+TGG 0.383618 8:+61330000 MsG0880045582.01.T01:CDS
AGCTTCAATCTGTTCTTAGA+AGG 0.390838 8:+61329560 MsG0880045582.01.T01:CDS
ATAAATGGTCATTAGTTTCA+AGG 0.411447 8:+61329837 MsG0880045582.01.T01:CDS
AAAACCATGTTGCTGAATAT+TGG 0.428191 8:+61329602 MsG0880045582.01.T01:CDS
TTGATGCAGAAGGGTGTGTT+TGG 0.432982 8:+61329860 MsG0880045582.01.T01:CDS
AAAAGGTCTAAGAATCTTAG+TGG 0.463444 8:-61329490 None:intergenic
GCTTTGGTGGTGGCTATTGA+AGG 0.475197 8:+61330016 MsG0880045582.01.T01:CDS
CTGAATATTGGTGTTTGAGA+AGG 0.492295 8:+61329614 MsG0880045582.01.T01:CDS
AAATGAGAATATTAATGAGT+TGG 0.492996 8:+61329895 MsG0880045582.01.T01:CDS
AGTTTCAAGGTTGATGCAGA+AGG 0.510581 8:+61329850 MsG0880045582.01.T01:CDS
TGGAAGGTTAGAAGCTTTGG+TGG 0.524132 8:+61330003 MsG0880045582.01.T01:CDS
TTTGAGAAGGAAAATGAGAA+AGG 0.532765 8:+61329627 MsG0880045582.01.T01:CDS
GTTTCAAGGTTGATGCAGAA+GGG 0.580414 8:+61329851 MsG0880045582.01.T01:CDS
AGATTCAATCTGCACATGGA+AGG 0.602686 8:+61329987 MsG0880045582.01.T01:CDS
AAGGTTAGAAGCTTTGGTGG+TGG 0.603606 8:+61330006 MsG0880045582.01.T01:CDS
AACACCAATATTCAGCAACA+TGG 0.620970 8:-61329606 None:intergenic
GAGAAGATTCAATCTGCACA+TGG 0.651774 8:+61329983 MsG0880045582.01.T01:CDS
GAATTTGAGTAAAGATGCTG+AGG 0.685171 8:+61329958 MsG0880045582.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGAGAATATTAATGAGT+TGG + Chr8:61329895-61329914 MsG0880045582.01.T01:CDS 20.0%
!!! TTTTGTTGAAACAAATTGAT+GGG + Chr8:61329668-61329687 MsG0880045582.01.T01:CDS 20.0%
! TGAGATCAAAGGTTAATAAA+TGG + Chr8:61329822-61329841 MsG0880045582.01.T01:intron 25.0%
! TTAACCTTTGATCTCAAAAT+TGG - Chr8:61329818-61329837 None:intergenic 25.0%
!! AGTTAGTAGTTTGATGATTA+TGG + Chr8:61329934-61329953 MsG0880045582.01.T01:CDS 25.0%
!! ATAAATGGTCATTAGTTTCA+AGG + Chr8:61329837-61329856 MsG0880045582.01.T01:CDS 25.0%
!! CTTTTGTTGAAACAAATTGA+TGG + Chr8:61329667-61329686 MsG0880045582.01.T01:CDS 25.0%
!!! TTGGATCATCATTTTTTCAT+TGG + Chr8:61329695-61329714 MsG0880045582.01.T01:intron 25.0%
AAAACCATGTTGCTGAATAT+TGG + Chr8:61329602-61329621 MsG0880045582.01.T01:CDS 30.0%
AAAAGGTCTAAGAATCTTAG+TGG - Chr8:61329493-61329512 None:intergenic 30.0%
GCTATTGAAGGAATTGAAAA+TGG + Chr8:61330028-61330047 MsG0880045582.01.T01:CDS 30.0%
GTTCTTAGAAGGAGAAAAAT+TGG + Chr8:61329571-61329590 MsG0880045582.01.T01:CDS 30.0%
TTTGAGAAGGAAAATGAGAA+AGG + Chr8:61329627-61329646 MsG0880045582.01.T01:CDS 30.0%
! AAACAAATTGATGGGTCAAT+TGG + Chr8:61329676-61329695 MsG0880045582.01.T01:intron 30.0%
! AAGGAGAAAAATTGGTGATA+TGG + Chr8:61329579-61329598 MsG0880045582.01.T01:CDS 30.0%
! GCACTTTTACAATTGAACAA+AGG - Chr8:61329734-61329753 None:intergenic 30.0%
!! GAGTTGGAAAAAGTTGATTT+TGG + Chr8:61329911-61329930 MsG0880045582.01.T01:CDS 30.0%
!! GATGATGTTTTGTTCATGAA+AGG + Chr8:61329526-61329545 MsG0880045582.01.T01:CDS 30.0%
!!! TTCACCAATTTTGAGATCAA+AGG + Chr8:61329811-61329830 MsG0880045582.01.T01:intron 30.0%
AACACCAATATTCAGCAACA+TGG - Chr8:61329609-61329628 None:intergenic 35.0%
AGCTTCAATCTGTTCTTAGA+AGG + Chr8:61329560-61329579 MsG0880045582.01.T01:CDS 35.0%
GAATTTGAGTAAAGATGCTG+AGG + Chr8:61329958-61329977 MsG0880045582.01.T01:CDS 35.0%
! CTGAATATTGGTGTTTGAGA+AGG + Chr8:61329614-61329633 MsG0880045582.01.T01:CDS 35.0%
!!! CATCTCTTGTTTTGCTCAAA+AGG - Chr8:61329510-61329529 None:intergenic 35.0%
ACATGGAAGGTTAGAAGCTT+TGG + Chr8:61330000-61330019 MsG0880045582.01.T01:CDS 40.0%
AGATTCAATCTGCACATGGA+AGG + Chr8:61329987-61330006 MsG0880045582.01.T01:CDS 40.0%
GAGAAGATTCAATCTGCACA+TGG + Chr8:61329983-61330002 MsG0880045582.01.T01:CDS 40.0%
!! AGTTTCAAGGTTGATGCAGA+AGG + Chr8:61329850-61329869 MsG0880045582.01.T01:CDS 40.0%
!! GTTTCAAGGTTGATGCAGAA+GGG + Chr8:61329851-61329870 MsG0880045582.01.T01:CDS 40.0%
! AAGGTTAGAAGCTTTGGTGG+TGG + Chr8:61330006-61330025 MsG0880045582.01.T01:CDS 45.0%
! TGGAAGGTTAGAAGCTTTGG+TGG + Chr8:61330003-61330022 MsG0880045582.01.T01:CDS 45.0%
! TTGATGCAGAAGGGTGTGTT+TGG + Chr8:61329860-61329879 MsG0880045582.01.T01:CDS 45.0%
!! GCTTTGGTGGTGGCTATTGA+AGG + Chr8:61330016-61330035 MsG0880045582.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 61329469 61330117 61329469 ID=MsG0880045582.01;Name=MsG0880045582.01
Chr8 mRNA 61329469 61330117 61329469 ID=MsG0880045582.01.T01;Parent=MsG0880045582.01;Name=MsG0880045582.01.T01;_AED=0.48;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|170
Chr8 exon 61329469 61329689 61329469 ID=MsG0880045582.01.T01:exon:12638;Parent=MsG0880045582.01.T01
Chr8 exon 61329826 61330117 61329826 ID=MsG0880045582.01.T01:exon:12639;Parent=MsG0880045582.01.T01
Chr8 CDS 61329469 61329689 61329469 ID=MsG0880045582.01.T01:cds;Parent=MsG0880045582.01.T01
Chr8 CDS 61329826 61330117 61329826 ID=MsG0880045582.01.T01:cds;Parent=MsG0880045582.01.T01
Gene Sequence

>MsG0880045582.01.T01

ATGGATGAGTTATTAGATTTTCCACTAAGATTCTTAGACCTTTTGAGCAAAACAAGAGATGATGTTTTGTTCATGAAAGGAAAAGTTGAAGAGCTTCAATCTGTTCTTAGAAGGAGAAAAATTGGTGATATGGAAAACCATGTTGCTGAATATTGGTGTTTGAGAAGGAAAATGAGAAAGGAATGTACAAAATCACTTCTTTTGTTGAAACAAATTGATGGATCAAAGGTTAATAAATGGTCATTAGTTTCAAGGTTGATGCAGAAGGGTGTGTTTGGTTGTGATAATCAAAATGAGAATATTAATGAGTTGGAAAAAGTTGATTTTGGAGTTAGTAGTTTGATGATTATGGAGAATTTGAGTAAAGATGCTGAGGCTGAGAAGATTCAATCTGCACATGGAAGGTTAGAAGCTTTGGTGGTGGCTATTGAAGGAATTGAAAATGGATTAGAATGTTTGTTTAAGCGATTGATTAATACTCGTGTGTCTTTTTTAAATATTATTTCTCCTTAG

Protein sequence

>MsG0880045582.01.T01

MDELLDFPLRFLDLLSKTRDDVLFMKGKVEELQSVLRRRKIGDMENHVAEYWCLRRKMRKECTKSLLLLKQIDGSKVNKWSLVSRLMQKGVFGCDNQNENINELEKVDFGVSSLMIMENLSKDAEAEKIQSAHGRLEALVVAIEGIENGLECLFKRLINTRVSFLNIISP*