Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046069.01.T01 | XP_024638785.1 | 98.571 | 70 | 1 | 0 | 28 | 97 | 537 | 606 | 3.60E-36 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046069.01.T01 | B9DFI7 | 69.118 | 68 | 21 | 0 | 28 | 95 | 542 | 609 | 4.58E-27 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047814.01 | MsG0880046069.01 | 0.806495 | 8.003133e-50 | 4.350122e-47 |
| MsG0080048856.01 | MsG0880046069.01 | 0.807210 | 5.650170e-50 | 3.128480e-47 |
| MsG0080048952.01 | MsG0880046069.01 | 0.831807 | 1.353831e-55 | 1.485479e-52 |
| MsG0180000001.01 | MsG0880046069.01 | 0.809880 | 1.520303e-50 | 9.031329e-48 |
| MsG0180000145.01 | MsG0880046069.01 | 0.811700 | 6.141003e-51 | 3.827500e-48 |
| MsG0180000178.01 | MsG0880046069.01 | 0.807557 | 4.767945e-50 | 2.663823e-47 |
| MsG0180000195.01 | MsG0880046069.01 | 0.826655 | 2.408431e-54 | 2.272645e-51 |
| MsG0180001173.01 | MsG0880046069.01 | 0.840922 | 6.494593e-58 | 9.390971e-55 |
| MsG0180001237.01 | MsG0880046069.01 | 0.811210 | 7.846160e-51 | 4.827723e-48 |
| MsG0180001565.01 | MsG0880046069.01 | 0.804039 | 2.616052e-49 | 1.335101e-46 |
| MsG0180001566.01 | MsG0880046069.01 | 0.835684 | 1.454240e-56 | 1.791546e-53 |
| MsG0880044565.01 | MsG0880046069.01 | 0.815584 | 8.577980e-52 | 5.937792e-49 |
| MsG0880044878.01 | MsG0880046069.01 | 0.812773 | 3.581522e-51 | 2.297251e-48 |
| MsG0880046012.01 | MsG0880046069.01 | 0.812531 | 4.046274e-51 | 2.579065e-48 |
| MsG0880046027.01 | MsG0880046069.01 | 0.851490 | 8.580015e-61 | 1.739491e-57 |
| MsG0880046069.01 | MsG0880046086.01 | 0.821123 | 4.773409e-53 | 3.849656e-50 |
| MsG0880046069.01 | MsG0880046479.01 | 0.804503 | 2.093484e-49 | 1.081169e-46 |
| MsG0180002952.01 | MsG0880046069.01 | 0.822104 | 2.831317e-53 | 2.347212e-50 |
| MsG0180004272.01 | MsG0880046069.01 | 0.804065 | 2.582726e-49 | 1.319049e-46 |
| MsG0180004463.01 | MsG0880046069.01 | 0.802018 | 6.846245e-49 | 3.317887e-46 |
| MsG0180005233.01 | MsG0880046069.01 | 0.809450 | 1.880549e-50 | 1.104468e-47 |
| MsG0180005763.01 | MsG0880046069.01 | 0.806564 | 7.738288e-50 | 4.213642e-47 |
| MsG0180006260.01 | MsG0880046069.01 | 0.804529 | 2.068065e-49 | 1.068754e-46 |
| MsG0780040261.01 | MsG0880046069.01 | 0.830305 | 3.164855e-55 | 3.321537e-52 |
| MsG0780040669.01 | MsG0880046069.01 | 0.822784 | 1.967808e-53 | 1.663394e-50 |
| MsG0780040926.01 | MsG0880046069.01 | 0.813623 | 2.330730e-51 | 1.529742e-48 |
| MsG0780041764.01 | MsG0880046069.01 | 0.835250 | 1.871424e-56 | 2.275829e-53 |
| MsG0880041986.01 | MsG0880046069.01 | 0.812360 | 4.409005e-51 | 2.797550e-48 |
| MsG0880042128.01 | MsG0880046069.01 | 0.801068 | 1.072152e-48 | 5.072748e-46 |
| MsG0880042520.01 | MsG0880046069.01 | 0.813573 | 2.390865e-51 | 1.567053e-48 |
| MsG0880042543.01 | MsG0880046069.01 | 0.803649 | 3.151282e-49 | 1.592175e-46 |
| MsG0880042698.01 | MsG0880046069.01 | 0.821164 | 4.670122e-53 | 3.770615e-50 |
| MsG0880042760.01 | MsG0880046069.01 | 0.803153 | 3.993037e-49 | 1.991990e-46 |
| MsG0880043491.01 | MsG0880046069.01 | 0.805142 | 1.539838e-49 | 8.084902e-47 |
| MsG0880043750.01 | MsG0880046069.01 | 0.809141 | 2.191416e-50 | 1.276506e-47 |
| MsG0280011090.01 | MsG0880046069.01 | 0.826821 | 2.198180e-54 | 2.084202e-51 |
| MsG0280011092.01 | MsG0880046069.01 | 0.826821 | 2.198180e-54 | 2.084202e-51 |
| MsG0280011293.01 | MsG0880046069.01 | 0.809763 | 1.611316e-50 | 9.543009e-48 |
| MsG0280011335.01 | MsG0880046069.01 | 0.835472 | 1.645535e-56 | 2.014188e-53 |
| MsG0380012460.01 | MsG0880046069.01 | 0.804704 | 1.901468e-49 | 9.871419e-47 |
| MsG0380013084.01 | MsG0880046069.01 | 0.801151 | 1.030999e-48 | 4.888280e-46 |
| MsG0380013475.01 | MsG0880046069.01 | 0.802927 | 4.447555e-49 | 2.205926e-46 |
| MsG0380013549.01 | MsG0880046069.01 | 0.838960 | 2.107741e-57 | 2.868273e-54 |
| MsG0380013834.01 | MsG0880046069.01 | 0.816621 | 5.033140e-52 | 3.584279e-49 |
| MsG0680030740.01 | MsG0880046069.01 | 0.803297 | 3.728133e-49 | 1.866654e-46 |
| MsG0680031975.01 | MsG0880046069.01 | 0.809036 | 2.306966e-50 | 1.340028e-47 |
| MsG0480018465.01 | MsG0880046069.01 | 0.807210 | 5.649892e-50 | 3.128328e-47 |
| MsG0480018513.01 | MsG0880046069.01 | 0.810350 | 1.203887e-50 | 7.240004e-48 |
| MsG0480019970.01 | MsG0880046069.01 | 0.814982 | 1.167343e-51 | 7.948526e-49 |
| MsG0480020634.01 | MsG0880046069.01 | 0.807541 | 4.805765e-50 | 2.683892e-47 |
| MsG0480021870.01 | MsG0880046069.01 | 0.802256 | 6.114495e-49 | 2.981366e-46 |
| MsG0480022094.01 | MsG0880046069.01 | 0.809182 | 2.147417e-50 | 1.252243e-47 |
| MsG0480022115.01 | MsG0880046069.01 | 0.831907 | 1.278751e-55 | 1.407280e-52 |
| MsG0480022228.01 | MsG0880046069.01 | 0.800850 | 1.187884e-48 | 5.589294e-46 |
| MsG0480022806.01 | MsG0880046069.01 | 0.807073 | 6.040996e-50 | 3.332824e-47 |
| MsG0480023172.01 | MsG0880046069.01 | 0.802231 | 6.187926e-49 | 3.015197e-46 |
| MsG0480023272.01 | MsG0880046069.01 | 0.817111 | 3.907268e-52 | 2.820015e-49 |
| MsG0480023307.01 | MsG0880046069.01 | 0.823767 | 1.160094e-53 | 1.008344e-50 |
| MsG0480023463.01 | MsG0880046069.01 | 0.850473 | 1.659524e-60 | 3.254400e-57 |
| MsG0480023488.01 | MsG0880046069.01 | 0.810073 | 1.381960e-50 | 8.250870e-48 |
| MsG0480023755.01 | MsG0880046069.01 | 0.836466 | 9.206356e-57 | 1.161203e-53 |
| MsG0480023873.01 | MsG0880046069.01 | 0.810235 | 1.274904e-50 | 7.643827e-48 |
| MsG0580024157.01 | MsG0880046069.01 | 0.821997 | 2.997771e-53 | 2.477724e-50 |
| MsG0580025352.01 | MsG0880046069.01 | 0.824676 | 7.094337e-54 | 6.327978e-51 |
| MsG0380015332.01 | MsG0880046069.01 | 0.813697 | 2.244479e-51 | 1.476092e-48 |
| MsG0380015363.01 | MsG0880046069.01 | 0.800214 | 1.601066e-48 | 7.412638e-46 |
| MsG0380015677.01 | MsG0880046069.01 | 0.804915 | 1.717476e-49 | 8.965840e-47 |
| MsG0380016236.01 | MsG0880046069.01 | 0.828378 | 9.297017e-55 | 9.224740e-52 |
| MsG0380016247.01 | MsG0880046069.01 | 0.829415 | 5.213141e-55 | 5.332185e-52 |
| MsG0380016882.01 | MsG0880046069.01 | 0.808435 | 3.101122e-50 | 1.773082e-47 |
| MsG0380016994.01 | MsG0880046069.01 | 0.827925 | 1.195302e-54 | 1.170386e-51 |
| MsG0380017335.01 | MsG0880046069.01 | 0.802852 | 4.608171e-49 | 2.281295e-46 |
| MsG0380017498.01 | MsG0880046069.01 | 0.812435 | 4.245621e-51 | 2.699415e-48 |
| MsG0380018075.01 | MsG0880046069.01 | 0.823929 | 1.062852e-53 | 9.279646e-51 |
| MsG0580028517.01 | MsG0880046069.01 | 0.800949 | 1.133635e-48 | 5.347593e-46 |
| MsG0580029926.01 | MsG0880046069.01 | 0.806966 | 6.362300e-50 | 3.500458e-47 |
| MsG0280007491.01 | MsG0880046069.01 | 0.800477 | 1.415191e-48 | 6.595810e-46 |
| MsG0280007529.01 | MsG0880046069.01 | 0.802009 | 6.874071e-49 | 3.330608e-46 |
| MsG0280008614.01 | MsG0880046069.01 | 0.814546 | 1.457991e-51 | 9.811717e-49 |
| MsG0280009514.01 | MsG0880046069.01 | 0.811430 | 7.027831e-51 | 4.349460e-48 |
| MsG0280009748.01 | MsG0880046069.01 | 0.853822 | 1.852810e-61 | 4.055009e-58 |
| MsG0280010109.01 | MsG0880046069.01 | 0.812139 | 4.926049e-51 | 3.106976e-48 |
| MsG0680035692.01 | MsG0880046069.01 | 0.803110 | 4.076479e-49 | 2.031257e-46 |
| MsG0780036018.01 | MsG0880046069.01 | 0.836161 | 1.100488e-56 | 1.375423e-53 |
| MsG0780036663.01 | MsG0880046069.01 | 0.813467 | 2.522441e-51 | 1.648635e-48 |
| MsG0780036752.01 | MsG0880046069.01 | 0.800127 | 1.667575e-48 | 7.703617e-46 |
| MsG0780037054.01 | MsG0880046069.01 | 0.813070 | 3.082512e-51 | 1.992883e-48 |
| MsG0780038051.01 | MsG0880046069.01 | 0.833665 | 4.680544e-56 | 5.426896e-53 |
| MsG0780038391.01 | MsG0880046069.01 | 0.817264 | 3.608553e-52 | 2.615309e-49 |
| MsG0780038443.01 | MsG0880046069.01 | 0.831107 | 2.012820e-55 | 2.162867e-52 |
| MsG0780038602.01 | MsG0880046069.01 | 0.813729 | 2.208936e-51 | 1.453979e-48 |
| MsG0780039243.01 | MsG0880046069.01 | 0.827451 | 1.553580e-54 | 1.500447e-51 |
| MsG0780039655.01 | MsG0880046069.01 | 0.826752 | 2.283528e-54 | 2.160755e-51 |
| MsG0780039656.01 | MsG0880046069.01 | 0.810575 | 1.076649e-50 | 6.513540e-48 |
| MsG0780039717.01 | MsG0880046069.01 | 0.806537 | 7.838373e-50 | 4.265272e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046069.01.T01 | MTR_4g098890 | 96.923 | 65 | 2 | 0 | 28 | 92 | 537 | 601 | 2.81e-35 | 133 |
| MsG0880046069.01.T01 | MTR_4g098890 | 70.455 | 44 | 4 | 1 | 130 | 164 | 1538 | 1581 | 7.40e-12 | 65.1 |
| MsG0880046069.01.T01 | MTR_5g040360 | 72.059 | 68 | 19 | 0 | 28 | 95 | 533 | 600 | 1.12e-28 | 113 |
| MsG0880046069.01.T01 | MTR_8g055840 | 73.529 | 68 | 18 | 0 | 28 | 95 | 534 | 601 | 1.56e-28 | 113 |
| MsG0880046069.01.T01 | MTR_3g040370 | 66.667 | 69 | 23 | 0 | 29 | 97 | 532 | 600 | 1.18e-25 | 105 |
| MsG0880046069.01.T01 | MTR_0019s0120 | 67.647 | 68 | 22 | 0 | 28 | 95 | 560 | 627 | 3.09e-25 | 103 |
| MsG0880046069.01.T01 | MTR_1g013180 | 38.849 | 139 | 66 | 3 | 97 | 216 | 15 | 153 | 1.12e-24 | 102 |
| MsG0880046069.01.T01 | MTR_1g101370 | 63.235 | 68 | 25 | 0 | 28 | 95 | 551 | 618 | 1.25e-23 | 99.4 |
| MsG0880046069.01.T01 | MTR_8g432680 | 60.870 | 69 | 27 | 0 | 28 | 96 | 564 | 632 | 5.68e-23 | 97.4 |
| MsG0880046069.01.T01 | MTR_7g076170 | 53.247 | 77 | 36 | 0 | 28 | 104 | 559 | 635 | 9.51e-23 | 96.7 |
| MsG0880046069.01.T01 | MTR_8g100075 | 53.846 | 78 | 33 | 1 | 28 | 105 | 554 | 628 | 2.10e-21 | 92.8 |
| MsG0880046069.01.T01 | MTR_8g432620 | 40.909 | 88 | 52 | 0 | 8 | 95 | 514 | 601 | 3.87e-16 | 77.4 |
| MsG0880046069.01.T01 | MTR_7g076150 | 41.379 | 87 | 51 | 0 | 8 | 94 | 358 | 444 | 8.54e-15 | 73.2 |
| MsG0880046069.01.T01 | MTR_7g076150 | 41.379 | 87 | 51 | 0 | 8 | 94 | 501 | 587 | 9.07e-15 | 73.6 |
| MsG0880046069.01.T01 | MTR_7g006060 | 50.769 | 65 | 29 | 1 | 28 | 89 | 502 | 566 | 1.14e-11 | 64.3 |
| MsG0880046069.01.T01 | MTR_3g096480 | 43.284 | 67 | 34 | 1 | 28 | 94 | 714 | 776 | 5.45e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880046069.01.T01 | AT1G26850 | 69.118 | 68 | 21 | 0 | 28 | 95 | 542 | 609 | 4.67e-28 | 112 |
| MsG0880046069.01.T01 | AT1G26850 | 69.118 | 68 | 21 | 0 | 28 | 95 | 542 | 609 | 4.67e-28 | 112 |
| MsG0880046069.01.T01 | AT4G18030 | 72.464 | 69 | 19 | 0 | 29 | 97 | 538 | 606 | 1.10e-27 | 110 |
| MsG0880046069.01.T01 | AT4G10440 | 67.143 | 70 | 23 | 0 | 28 | 97 | 550 | 619 | 2.73e-26 | 107 |
| MsG0880046069.01.T01 | AT4G00750 | 63.768 | 69 | 25 | 0 | 28 | 96 | 556 | 624 | 2.40e-24 | 101 |
| MsG0880046069.01.T01 | AT1G33170 | 60.870 | 69 | 27 | 0 | 28 | 96 | 568 | 636 | 2.16e-23 | 98.6 |
| MsG0880046069.01.T01 | AT2G45750 | 58.824 | 68 | 28 | 0 | 28 | 95 | 549 | 616 | 6.54e-21 | 91.3 |
| MsG0880046069.01.T01 | AT4G00740 | 44.776 | 67 | 37 | 0 | 28 | 94 | 527 | 593 | 4.25e-13 | 68.6 |
| MsG0880046069.01.T01 | AT4G00740 | 44.776 | 67 | 37 | 0 | 28 | 94 | 527 | 593 | 4.25e-13 | 68.6 |
| MsG0880046069.01.T01 | AT2G39750 | 45.312 | 64 | 35 | 0 | 28 | 91 | 626 | 689 | 2.64e-12 | 66.2 |
| MsG0880046069.01.T01 | AT2G43200 | 50.000 | 62 | 31 | 0 | 28 | 89 | 545 | 606 | 7.19e-12 | 65.1 |
| MsG0880046069.01.T01 | AT2G43200 | 50.000 | 62 | 31 | 0 | 28 | 89 | 545 | 606 | 7.19e-12 | 65.1 |
| MsG0880046069.01.T01 | AT2G34300 | 41.791 | 67 | 35 | 1 | 28 | 94 | 695 | 757 | 2.98e-11 | 63.2 |
| MsG0880046069.01.T01 | AT2G34300 | 41.791 | 67 | 35 | 1 | 28 | 94 | 695 | 757 | 2.98e-11 | 63.2 |
| MsG0880046069.01.T01 | AT2G34300 | 41.791 | 67 | 35 | 1 | 28 | 94 | 695 | 757 | 2.98e-11 | 63.2 |
| MsG0880046069.01.T01 | AT1G29470 | 38.806 | 67 | 37 | 1 | 28 | 94 | 695 | 757 | 3.46e-11 | 63.2 |
| MsG0880046069.01.T01 | AT1G29470 | 38.806 | 67 | 37 | 1 | 28 | 94 | 695 | 757 | 3.46e-11 | 63.2 |
| MsG0880046069.01.T01 | AT5G06050 | 39.394 | 66 | 40 | 0 | 28 | 93 | 598 | 663 | 3.72e-11 | 62.8 |
Find 62 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCGAGAAATTATGTTCTT+TGG | 0.191572 | 8:-68594455 | MsG0880046069.01.T01:CDS |
| TTCACAAATAAAGGCTGTTT+TGG | 0.258338 | 8:-68594324 | MsG0880046069.01.T01:CDS |
| TAGCTATTTGAATGGCTAAT+TGG | 0.289695 | 8:+68594202 | None:intergenic |
| ACAAGGTTCAGAAAATTGTT+AGG | 0.303090 | 8:-68595640 | MsG0880046069.01.T01:CDS |
| TAGAGATGGACCGCATATTA+AGG | 0.304977 | 8:-68595706 | MsG0880046069.01.T01:CDS |
| TCTCTAAAAGGATGTCTTCT+AGG | 0.313204 | 8:+68595723 | None:intergenic |
| ATGTGAAACCAGCTCCTAAT+TGG | 0.317448 | 8:-68594374 | MsG0880046069.01.T01:CDS |
| ATCGAGAAATTATGTTCTTT+GGG | 0.321597 | 8:-68594454 | MsG0880046069.01.T01:CDS |
| ATATGCGGTCCATCTCTAAA+AGG | 0.333621 | 8:+68595711 | None:intergenic |
| TACAATAACCAATATCTTCT+CGG | 0.340229 | 8:+68595565 | None:intergenic |
| GTTGTTAATGTGGTGCTTAA+TGG | 0.340279 | 8:-68594145 | MsG0880046069.01.T01:CDS |
| CTGCAATGTTGTATAGAATA+TGG | 0.344455 | 8:-68594479 | MsG0880046069.01.T01:CDS |
| GCACCACATTAACAACATAT+TGG | 0.347917 | 8:+68594152 | None:intergenic |
| GGTTATTGTAAAAGAGTATT+GGG | 0.355588 | 8:-68595552 | MsG0880046069.01.T01:CDS |
| ATTTGCACCCGAGAAGATAT+TGG | 0.359714 | 8:-68595573 | MsG0880046069.01.T01:CDS |
| AATGAGATGGGAATCTAAAA+TGG | 0.360431 | 8:-68595615 | MsG0880046069.01.T01:CDS |
| TTTGGAGTCAAAATTTGTTC+CGG | 0.373190 | 8:-68594306 | MsG0880046069.01.T01:CDS |
| TTGGAGTCAAAATTTGTTCC+GGG | 0.381010 | 8:-68594305 | MsG0880046069.01.T01:CDS |
| CGGATTATGACCGATCCTTC+TGG | 0.383497 | 8:+68595683 | None:intergenic |
| ACGTTACACTTCAACCAATT+AGG | 0.385496 | 8:+68594360 | None:intergenic |
| AATATCTTCTCGGGTGCAAA+TGG | 0.387847 | 8:+68595575 | None:intergenic |
| TCATTGGATCACCCACCATT+AGG | 0.389491 | 8:-68595881 | MsG0880046069.01.T01:CDS |
| CAAGGTTCAGAAAATTGTTA+GGG | 0.396641 | 8:-68595639 | MsG0880046069.01.T01:CDS |
| AAGTCTATTCAAGATTTCAT+TGG | 0.429500 | 8:-68595897 | MsG0880046069.01.T01:CDS |
| ATACAACATTGCAGCAAAAC+GGG | 0.429972 | 8:+68594488 | None:intergenic |
| AGAAGAGCATTGCGCGCGCT+TGG | 0.439978 | 8:+68594262 | None:intergenic |
| TGGTTATTGTAAAAGAGTAT+TGG | 0.449942 | 8:-68595553 | MsG0880046069.01.T01:CDS |
| ACAATAACCAATATCTTCTC+GGG | 0.458082 | 8:+68595566 | None:intergenic |
| GACTGGAAGCCTGAGCCAAC+TGG | 0.468297 | 8:+68595843 | None:intergenic |
| TATACAACATTGCAGCAAAA+CGG | 0.468539 | 8:+68594487 | None:intergenic |
| CACTTCAACCAATTAGGAGC+TGG | 0.481244 | 8:+68594366 | None:intergenic |
| CATGTACGTAGCTATTTGAA+TGG | 0.483146 | 8:+68594194 | None:intergenic |
| TCCGATAGCAGGAGACCTAA+TGG | 0.489099 | 8:+68595866 | None:intergenic |
| ATTGTAAAAGAGTATTGGGT+TGG | 0.493911 | 8:-68595548 | MsG0880046069.01.T01:CDS |
| GAGCCAACTGGTCCGATAGC+AGG | 0.509004 | 8:+68595855 | None:intergenic |
| AATTGTTAGGGGAATGAGAT+GGG | 0.515509 | 8:-68595627 | MsG0880046069.01.T01:CDS |
| GCTATCGGACCAGTTGGCTC+AGG | 0.518335 | 8:-68595852 | MsG0880046069.01.T01:CDS |
| ACCATTAGGTCTCCTGCTAT+CGG | 0.537198 | 8:-68595867 | MsG0880046069.01.T01:CDS |
| ATCTAAAATGGTGGATCATG+AGG | 0.541586 | 8:-68595603 | MsG0880046069.01.T01:CDS |
| ATATCTTCTCGGGTGCAAAT+GGG | 0.548383 | 8:+68595576 | None:intergenic |
| CATATTAAGGCCAGAAGGAT+CGG | 0.556558 | 8:-68595693 | MsG0880046069.01.T01:CDS |
| TCTCCTGCTATCGGACCAGT+TGG | 0.568974 | 8:-68595858 | MsG0880046069.01.T01:CDS |
| ATCCTTCTGGCCTTAATATG+CGG | 0.578717 | 8:+68595696 | None:intergenic |
| AAACCAATATGTTGTTAATG+TGG | 0.585139 | 8:-68594155 | MsG0880046069.01.T01:CDS |
| TGAATGTTGATGCAACCTGC+AGG | 0.591696 | 8:-68594419 | MsG0880046069.01.T01:CDS |
| CGAAGTTGATGTGATTAACA+AGG | 0.596587 | 8:-68595657 | MsG0880046069.01.T01:CDS |
| TAGCCATGTGATTGAATGCG+AGG | 0.600151 | 8:-68594232 | MsG0880046069.01.T01:CDS |
| AGCATGTGATGTAATCCTGC+AGG | 0.606187 | 8:+68594404 | None:intergenic |
| AAAACGACTCATATTATCCC+CGG | 0.606515 | 8:+68594287 | None:intergenic |
| AAATTGTTAGGGGAATGAGA+TGG | 0.612828 | 8:-68595628 | MsG0880046069.01.T01:CDS |
| GAGATGGGAATCTAAAATGG+TGG | 0.616902 | 8:-68595612 | MsG0880046069.01.T01:CDS |
| AAAATGGTGGATCATGAGGA+TGG | 0.624067 | 8:-68595599 | MsG0880046069.01.T01:CDS |
| GCATTCAATCACATGGCTAA+CGG | 0.626538 | 8:+68594236 | None:intergenic |
| TGTAATCATGAGAATGAACT+TGG | 0.630157 | 8:-68594121 | MsG0880046069.01.T01:CDS |
| TGGAGTCAAAATTTGTTCCG+GGG | 0.630174 | 8:-68594304 | MsG0880046069.01.T01:CDS |
| GACCGCATATTAAGGCCAGA+AGG | 0.646017 | 8:-68595698 | MsG0880046069.01.T01:CDS |
| TAACCTCGCATTCAATCACA+TGG | 0.669776 | 8:+68594229 | None:intergenic |
| ATAGCAGGAGACCTAATGGT+GGG | 0.671374 | 8:+68595870 | None:intergenic |
| AAGGTTCAGAAAATTGTTAG+GGG | 0.677847 | 8:-68595638 | MsG0880046069.01.T01:CDS |
| TAATTGGTTGAAGTGTAACG+TGG | 0.697218 | 8:-68594358 | MsG0880046069.01.T01:CDS |
| GATAGCAGGAGACCTAATGG+TGG | 0.705442 | 8:+68595869 | None:intergenic |
| TAATCACATCAACTTCGTCG+CGG | 0.731217 | 8:+68595663 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTTTTTTTTTTTTTTG+AGG | - | Chr8:68595397-68595416 | MsG0880046069.01.T01:intron | 10.0% |
| !!! | CTTTTTTTTTTTTTTTTTGA+GGG | - | Chr8:68595398-68595417 | MsG0880046069.01.T01:intron | 10.0% |
| !! | ACAATGTTGAAAAAATAATT+CGG | + | Chr8:68595485-68595504 | None:intergenic | 15.0% |
| !! | ATAATAATAATAAGATACAG+AGG | + | Chr8:68595069-68595088 | None:intergenic | 15.0% |
| !! | TAATAATAATAAGATACAGA+GGG | + | Chr8:68595068-68595087 | None:intergenic | 15.0% |
| !!! | ATATATTGGCTAAAATTATT+TGG | + | Chr8:68594793-68594812 | None:intergenic | 15.0% |
| !!! | TAGTTTTCTTTAATTGATTT+TGG | - | Chr8:68594735-68594754 | MsG0880046069.01.T01:intron | 15.0% |
| !!! | TATATTGGCTAAAATTATTT+GGG | + | Chr8:68594792-68594811 | None:intergenic | 15.0% |
| !! | CAATGTTGAAAAAATAATTC+GGG | + | Chr8:68595484-68595503 | None:intergenic | 20.0% |
| !! | TATGCTATTATTAACTGTAT+GGG | + | Chr8:68595185-68595204 | None:intergenic | 20.0% |
| !! | TCAACATCAACAAAATATAT+TGG | + | Chr8:68594807-68594826 | None:intergenic | 20.0% |
| !! | TTATGCTATTATTAACTGTA+TGG | + | Chr8:68595186-68595205 | None:intergenic | 20.0% |
| !!! | TTTTCTTTAATTGATTTTGG+AGG | - | Chr8:68594738-68594757 | MsG0880046069.01.T01:intron | 20.0% |
| !!! | TTTTTTTTTCCTAGTAGATT+AGG | + | Chr8:68594201-68594220 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTTTTTTGAGGG+AGG | - | Chr8:68595401-68595420 | MsG0880046069.01.T01:intron | 20.0% |
| ! | AAACCAATATGTTGTTAATG+TGG | - | Chr8:68595864-68595883 | MsG0880046069.01.T01:CDS | 25.0% |
| ! | AAGTCTATTCAAGATTTCAT+TGG | - | Chr8:68594122-68594141 | MsG0880046069.01.T01:CDS | 25.0% |
| ! | ATCGAGAAATTATGTTCTTT+GGG | - | Chr8:68595565-68595584 | MsG0880046069.01.T01:CDS | 25.0% |
| ! | TACAATAACCAATATCTTCT+CGG | + | Chr8:68594457-68594476 | None:intergenic | 25.0% |
| ! | TATCGAGAAATTATGTTCTT+TGG | - | Chr8:68595564-68595583 | MsG0880046069.01.T01:CDS | 25.0% |
| ! | TATGATATTCACATGTTCTT+TGG | - | Chr8:68595339-68595358 | MsG0880046069.01.T01:intron | 25.0% |
| !! | TGGTTATTGTAAAAGAGTAT+TGG | - | Chr8:68594466-68594485 | MsG0880046069.01.T01:CDS | 25.0% |
| !!! | GGTTATTGTAAAAGAGTATT+GGG | - | Chr8:68594467-68594486 | MsG0880046069.01.T01:CDS | 25.0% |
| !!! | TATTTTTTCAACATTGTTGC+AGG | - | Chr8:68595488-68595507 | MsG0880046069.01.T01:intron | 25.0% |
| AAATAACAAACCACATGTGT+AGG | + | Chr8:68595039-68595058 | None:intergenic | 30.0% | |
| AAGGTTCAGAAAATTGTTAG+GGG | - | Chr8:68594381-68594400 | MsG0880046069.01.T01:CDS | 30.0% | |
| AATGAGATGGGAATCTAAAA+TGG | - | Chr8:68594404-68594423 | MsG0880046069.01.T01:CDS | 30.0% | |
| ACAAGGTTCAGAAAATTGTT+AGG | - | Chr8:68594379-68594398 | MsG0880046069.01.T01:CDS | 30.0% | |
| ACAATAACCAATATCTTCTC+GGG | + | Chr8:68594456-68594475 | None:intergenic | 30.0% | |
| ACAGTTCTACACTTGATTAA+AGG | + | Chr8:68594691-68594710 | None:intergenic | 30.0% | |
| AGAAGAAATGGAAAAGTTGA+TGG | - | Chr8:68594592-68594611 | MsG0880046069.01.T01:intron | 30.0% | |
| ATTGAGAAAATTGAGAGAAG+TGG | - | Chr8:68595112-68595131 | MsG0880046069.01.T01:intron | 30.0% | |
| CAAGGTTCAGAAAATTGTTA+GGG | - | Chr8:68594380-68594399 | MsG0880046069.01.T01:CDS | 30.0% | |
| CATGAATAGTCATCAAAGAT+TGG | - | Chr8:68594535-68594554 | MsG0880046069.01.T01:intron | 30.0% | |
| CTGCAATGTTGTATAGAATA+TGG | - | Chr8:68595540-68595559 | MsG0880046069.01.T01:intron | 30.0% | |
| TAGCTATTTGAATGGCTAAT+TGG | + | Chr8:68595820-68595839 | None:intergenic | 30.0% | |
| TATACAACATTGCAGCAAAA+CGG | + | Chr8:68595535-68595554 | None:intergenic | 30.0% | |
| TCAATACTGTGAAACTTGTA+GGG | + | Chr8:68595097-68595116 | None:intergenic | 30.0% | |
| TGTAATCATGAGAATGAACT+TGG | - | Chr8:68595898-68595917 | MsG0880046069.01.T01:CDS | 30.0% | |
| TTCCATTTCTTCTTTGCTTT+GGG | + | Chr8:68594585-68594604 | None:intergenic | 30.0% | |
| TTTCCATTTCTTCTTTGCTT+TGG | + | Chr8:68594586-68594605 | None:intergenic | 30.0% | |
| ! | TCATTTTACCAAGTTTGGTA+TGG | - | Chr8:68594907-68594926 | MsG0880046069.01.T01:intron | 30.0% |
| ! | TGTGTTCATTTTACCAAGTT+TGG | - | Chr8:68594902-68594921 | MsG0880046069.01.T01:intron | 30.0% |
| ! | TTCACAAATAAAGGCTGTTT+TGG | - | Chr8:68595695-68595714 | MsG0880046069.01.T01:CDS | 30.0% |
| ! | TTTGGAGTCAAAATTTGTTC+CGG | - | Chr8:68595713-68595732 | MsG0880046069.01.T01:CDS | 30.0% |
| !! | ATTGTAAAAGAGTATTGGGT+TGG | - | Chr8:68594471-68594490 | MsG0880046069.01.T01:CDS | 30.0% |
| !! | GTATTGTTGATTTGATTGAC+AGG | - | Chr8:68594268-68594287 | MsG0880046069.01.T01:CDS | 30.0% |
| !!! | TGTTCTTTTAGAACTCTAAC+TGG | + | Chr8:68594517-68594536 | None:intergenic | 30.0% |
| AAAACGACTCATATTATCCC+CGG | + | Chr8:68595735-68595754 | None:intergenic | 35.0% | |
| AAATTGTTAGGGGAATGAGA+TGG | - | Chr8:68594391-68594410 | MsG0880046069.01.T01:CDS | 35.0% | |
| AACCAATTGCAAGCAAAAGT+CGG | - | Chr8:68594641-68594660 | MsG0880046069.01.T01:intron | 35.0% | |
| AACCCAAAGCAAAGAAGAAA+TGG | - | Chr8:68594580-68594599 | MsG0880046069.01.T01:intron | 35.0% | |
| AATTGTTAGGGGAATGAGAT+GGG | - | Chr8:68594392-68594411 | MsG0880046069.01.T01:CDS | 35.0% | |
| ACGTTACACTTCAACCAATT+AGG | + | Chr8:68595662-68595681 | None:intergenic | 35.0% | |
| AGCTTTGCAAAGTTCAAGTA+TGG | - | Chr8:68594993-68595012 | MsG0880046069.01.T01:intron | 35.0% | |
| ATACAACATTGCAGCAAAAC+GGG | + | Chr8:68595534-68595553 | None:intergenic | 35.0% | |
| CATGTACGTAGCTATTTGAA+TGG | + | Chr8:68595828-68595847 | None:intergenic | 35.0% | |
| CTCAATACTGTGAAACTTGT+AGG | + | Chr8:68595098-68595117 | None:intergenic | 35.0% | |
| GCACCACATTAACAACATAT+TGG | + | Chr8:68595870-68595889 | None:intergenic | 35.0% | |
| GTTGTTAATGTGGTGCTTAA+TGG | - | Chr8:68595874-68595893 | MsG0880046069.01.T01:CDS | 35.0% | |
| TAATTGGTTGAAGTGTAACG+TGG | - | Chr8:68595661-68595680 | MsG0880046069.01.T01:CDS | 35.0% | |
| TGAACTTTGCAAAGCTAAGT+AGG | + | Chr8:68594989-68595008 | None:intergenic | 35.0% | |
| TGGCTAAAATTATTTGGGTG+AGG | + | Chr8:68594787-68594806 | None:intergenic | 35.0% | |
| ! | TACCAAGTTTGGTATGGTTT+TGG | - | Chr8:68594913-68594932 | MsG0880046069.01.T01:intron | 35.0% |
| ! | TCTCTAAAAGGATGTCTTCT+AGG | + | Chr8:68594299-68594318 | None:intergenic | 35.0% |
| ! | TGCGCTCTTTTCACAAATAA+AGG | - | Chr8:68595686-68595705 | MsG0880046069.01.T01:CDS | 35.0% |
| ! | TTGGAGTCAAAATTTGTTCC+GGG | - | Chr8:68595714-68595733 | MsG0880046069.01.T01:CDS | 35.0% |
| ! | TTTTCCTAGTAGATTAGGAC+TGG | + | Chr8:68594196-68594215 | None:intergenic | 35.0% |
| !! | AGAAGACATCCTTTTAGAGA+TGG | - | Chr8:68594299-68594318 | MsG0880046069.01.T01:CDS | 35.0% |
| !! | ATCTAAAATGGTGGATCATG+AGG | - | Chr8:68594416-68594435 | MsG0880046069.01.T01:CDS | 35.0% |
| !! | CGAAGTTGATGTGATTAACA+AGG | - | Chr8:68594362-68594381 | MsG0880046069.01.T01:CDS | 35.0% |
| AAAGTTCAAGTATGGCTCCA+TGG | - | Chr8:68595001-68595020 | MsG0880046069.01.T01:intron | 40.0% | |
| AAATTCAAGGCCTACACATG+TGG | - | Chr8:68595026-68595045 | MsG0880046069.01.T01:intron | 40.0% | |
| AATATCTTCTCGGGTGCAAA+TGG | + | Chr8:68594447-68594466 | None:intergenic | 40.0% | |
| ATATCTTCTCGGGTGCAAAT+GGG | + | Chr8:68594446-68594465 | None:intergenic | 40.0% | |
| ATATGCGGTCCATCTCTAAA+AGG | + | Chr8:68594311-68594330 | None:intergenic | 40.0% | |
| ATGTGAAACCAGCTCCTAAT+TGG | - | Chr8:68595645-68595664 | MsG0880046069.01.T01:CDS | 40.0% | |
| ATTTGCACCCGAGAAGATAT+TGG | - | Chr8:68594446-68594465 | MsG0880046069.01.T01:CDS | 40.0% | |
| CACACATCACACATGTCATT+TGG | + | Chr8:68595305-68595324 | None:intergenic | 40.0% | |
| CATATTAAGGCCAGAAGGAT+CGG | - | Chr8:68594326-68594345 | MsG0880046069.01.T01:CDS | 40.0% | |
| CTCCAAAACCATACCAAACT+TGG | + | Chr8:68594918-68594937 | None:intergenic | 40.0% | |
| GAACTTCTATTGCTCGAATG+AGG | - | Chr8:68595208-68595227 | MsG0880046069.01.T01:intron | 40.0% | |
| GCATTCAATCACATGGCTAA+CGG | + | Chr8:68595786-68595805 | None:intergenic | 40.0% | |
| GGAGGAATGCAATAAACTTG+TGG | - | Chr8:68595419-68595438 | MsG0880046069.01.T01:intron | 40.0% | |
| GTGCTCACTACAAAGATTGT+TGG | - | Chr8:68595364-68595383 | MsG0880046069.01.T01:intron | 40.0% | |
| TAACCTCGCATTCAATCACA+TGG | + | Chr8:68595793-68595812 | None:intergenic | 40.0% | |
| TAATCACATCAACTTCGTCG+CGG | + | Chr8:68594359-68594378 | None:intergenic | 40.0% | |
| TAGAGATGGACCGCATATTA+AGG | - | Chr8:68594313-68594332 | MsG0880046069.01.T01:CDS | 40.0% | |
| TGGCTCCATGGAAAAATTCA+AGG | - | Chr8:68595013-68595032 | MsG0880046069.01.T01:intron | 40.0% | |
| TTGATTGTTGCCATTACCTC+TGG | - | Chr8:68595232-68595251 | MsG0880046069.01.T01:intron | 40.0% | |
| TTTGGAAAAAGACACCTCCA+TGG | + | Chr8:68595287-68595306 | None:intergenic | 40.0% | |
| ! | GAGATGGGAATCTAAAATGG+TGG | - | Chr8:68594407-68594426 | MsG0880046069.01.T01:CDS | 40.0% |
| ! | GTAGGCCTTGAATTTTTCCA+TGG | + | Chr8:68595021-68595040 | None:intergenic | 40.0% |
| ! | TGGAGTCAAAATTTGTTCCG+GGG | - | Chr8:68595715-68595734 | MsG0880046069.01.T01:CDS | 40.0% |
| !! | AAAATGGTGGATCATGAGGA+TGG | - | Chr8:68594420-68594439 | MsG0880046069.01.T01:CDS | 40.0% |
| !! | AACCGACTTTTGCTTGCAAT+TGG | + | Chr8:68594646-68594665 | None:intergenic | 40.0% |
| !! | ATCCTTCTGGCCTTAATATG+CGG | + | Chr8:68594326-68594345 | None:intergenic | 40.0% |
| !!! | AGTTTGGTATGGTTTTGGAG+AGG | - | Chr8:68594918-68594937 | MsG0880046069.01.T01:intron | 40.0% |
| ACCATTAGGTCTCCTGCTAT+CGG | - | Chr8:68594152-68594171 | MsG0880046069.01.T01:CDS | 45.0% | |
| AGCATGTGATGTAATCCTGC+AGG | + | Chr8:68595618-68595637 | None:intergenic | 45.0% | |
| ATAGCAGGAGACCTAATGGT+GGG | + | Chr8:68594152-68594171 | None:intergenic | 45.0% | |
| CACTTCAACCAATTAGGAGC+TGG | + | Chr8:68595656-68595675 | None:intergenic | 45.0% | |
| GAAGGCAAAACCAGAGGTAA+TGG | + | Chr8:68595245-68595264 | None:intergenic | 45.0% | |
| GCTTCCAGTCCTAATCTACT+AGG | - | Chr8:68594189-68594208 | MsG0880046069.01.T01:CDS | 45.0% | |
| TAGCCATGTGATTGAATGCG+AGG | - | Chr8:68595787-68595806 | MsG0880046069.01.T01:intron | 45.0% | |
| TCATTGGATCACCCACCATT+AGG | - | Chr8:68594138-68594157 | MsG0880046069.01.T01:CDS | 45.0% | |
| TCGGGAAAAGTCGTTGCAAA+AGG | + | Chr8:68595466-68595485 | None:intergenic | 45.0% | |
| TGAATGTTGATGCAACCTGC+AGG | - | Chr8:68595600-68595619 | MsG0880046069.01.T01:CDS | 45.0% | |
| !!! | ATGGTTTTGGAGAGGATGAG+AGG | - | Chr8:68594926-68594945 | MsG0880046069.01.T01:intron | 45.0% |
| AAAAGACACCTCCATGGTGC+AGG | + | Chr8:68595281-68595300 | None:intergenic | 50.0% | |
| ACCTGATTCAACCTGCACCA+TGG | - | Chr8:68595267-68595286 | MsG0880046069.01.T01:intron | 50.0% | |
| CGGATTATGACCGATCCTTC+TGG | + | Chr8:68594339-68594358 | None:intergenic | 50.0% | |
| GACCGCATATTAAGGCCAGA+AGG | - | Chr8:68594321-68594340 | MsG0880046069.01.T01:CDS | 50.0% | |
| GATAGCAGGAGACCTAATGG+TGG | + | Chr8:68594153-68594172 | None:intergenic | 50.0% | |
| TCCGATAGCAGGAGACCTAA+TGG | + | Chr8:68594156-68594175 | None:intergenic | 50.0% | |
| TGATTCAACCTGCACCATGG+AGG | - | Chr8:68595270-68595289 | MsG0880046069.01.T01:intron | 50.0% | |
| ! | ATGGTGCAGGTTGAATCAGG+TGG | + | Chr8:68595268-68595287 | None:intergenic | 50.0% |
| ! | TCCATGGTGCAGGTTGAATC+AGG | + | Chr8:68595271-68595290 | None:intergenic | 50.0% |
| ! | TGGTGCAGGTTGAATCAGGT+GGG | + | Chr8:68595267-68595286 | None:intergenic | 50.0% |
| AGGTGGGAAGGCAAAACCAG+AGG | + | Chr8:68595251-68595270 | None:intergenic | 55.0% | |
| TCTCCTGCTATCGGACCAGT+TGG | - | Chr8:68594161-68594180 | MsG0880046069.01.T01:CDS | 55.0% | |
| ! | GCAGGTTGAATCAGGTGGGA+AGG | + | Chr8:68595263-68595282 | None:intergenic | 55.0% |
| GACTGGAAGCCTGAGCCAAC+TGG | + | Chr8:68594179-68594198 | None:intergenic | 60.0% | |
| GAGCCAACTGGTCCGATAGC+AGG | + | Chr8:68594167-68594186 | None:intergenic | 60.0% | |
| ! | GCTATCGGACCAGTTGGCTC+AGG | - | Chr8:68594167-68594186 | MsG0880046069.01.T01:CDS | 60.0% |
| !! | AGAAGAGCATTGCGCGCGCT+TGG | + | Chr8:68595760-68595779 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 68594113 | 68595928 | 68594113 | ID=MsG0880046069.01;Name=MsG0880046069.01 |
| Chr8 | mRNA | 68594113 | 68595928 | 68594113 | ID=MsG0880046069.01.T01;Parent=MsG0880046069.01;Name=MsG0880046069.01.T01;_AED=0.77;_eAED=0.83;_QI=0|0|0|0.66|1|0.66|3|0|236 |
| Chr8 | exon | 68595846 | 68595928 | 68595846 | ID=MsG0880046069.01.T01:exon:12910;Parent=MsG0880046069.01.T01 |
| Chr8 | exon | 68595543 | 68595751 | 68595543 | ID=MsG0880046069.01.T01:exon:12909;Parent=MsG0880046069.01.T01 |
| Chr8 | exon | 68594113 | 68594531 | 68594113 | ID=MsG0880046069.01.T01:exon:12908;Parent=MsG0880046069.01.T01 |
| Chr8 | CDS | 68595846 | 68595928 | 68595846 | ID=MsG0880046069.01.T01:cds;Parent=MsG0880046069.01.T01 |
| Chr8 | CDS | 68595543 | 68595751 | 68595543 | ID=MsG0880046069.01.T01:cds;Parent=MsG0880046069.01.T01 |
| Chr8 | CDS | 68594113 | 68594531 | 68594113 | ID=MsG0880046069.01.T01:cds;Parent=MsG0880046069.01.T01 |
| Gene Sequence |
| Protein sequence |
