AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0152480


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0152480 MtrunA17_Chr1g0161551 92.857 84 5 1 1 84 1 83 2.95e-50 154
Msa0152480 MtrunA17_Chr6g0479181 41.228 114 63 2 9 122 53 162 3.21e-23 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0152480 AT1G14760.2 42.157 102 54 3 22 119 32 132 1.44e-19 78.6
Msa0152480 AT1G70510.1 36.792 106 59 2 22 122 68 170 6.75e-12 61.2
Msa0152480 AT1G23380.1 34.906 106 61 2 22 122 84 186 2.31e-11 59.7

Find 19 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGATGGAGGGTAAAGAAAT+TGG 0.288718 1_4:+15158837 None:intergenic
GTTTGGACTAGTTGTTGTTT+TGG 0.335695 1_4:-15159526 None:intergenic
CCATCCTTTGTTTGAAGCAT+TGG 0.359729 1_4:+15158926 Msa0152480:CDS
TGATCAAGCTCTGAATTGTT+TGG 0.360004 1_4:-15159543 None:intergenic
TTCAGAGCTTGATCACTTCA+TGG 0.367274 1_4:+15159551 Msa0152480:CDS
AAGTGATGCATGGAAGAAAT+TGG 0.438853 1_4:+15159491 Msa0152480:CDS
GTCTCACATCAATTGTTTGA+AGG 0.470627 1_4:+15158953 Msa0152480:CDS
TATATTCAACTGCAGGAACT+TGG 0.501461 1_4:+15160800 Msa0152480:CDS
GTTCATCAATCACGTCTTTG+AGG 0.505826 1_4:-15160742 None:intergenic
AAAAGAAAGTGAAAATGAAG+AGG 0.568561 1_4:+15158881 Msa0152480:CDS
CCAATGCTTCAAACAAAGGA+TGG 0.572089 1_4:-15158926 None:intergenic
TTGATATAACAAGTGATGCA+TGG 0.576358 1_4:+15159481 Msa0152480:CDS
GGAAAGGGAAGTGATGATGT+AGG 0.576930 1_4:+15158858 Msa0152480:CDS
TGCTACGTATATTCAACTGC+AGG 0.578517 1_4:+15160793 Msa0152480:CDS
TCAACCAATGCTTCAAACAA+AGG 0.579326 1_4:-15158930 None:intergenic
GAGGGTAAAGAAATTGGAAA+GGG 0.582588 1_4:+15158843 Msa0152480:CDS
GGAGGGTAAAGAAATTGGAA+AGG 0.606364 1_4:+15158842 Msa0152480:CDS
TATTTCTTAGGTCGGATCTG+AGG 0.611391 1_4:+15159446 Msa0152480:intron
CAACTGCAGGAACTTGGTGA+AGG 0.630993 1_4:+15160806 Msa0152480:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATATAGTGTATAATTA+TGG + chr1_4:15159100-15159119 Msa0152480:intron 10.0%
!! ATATTAATATATACACAAAA+AGG + chr1_4:15159177-15159196 Msa0152480:intron 10.0%
!! TATTAATATATACACAAAAA+GGG + chr1_4:15159178-15159197 Msa0152480:intron 10.0%
!! ATAGATATATAAATTCTTTA+CGG + chr1_4:15159777-15159796 Msa0152480:intron 10.0%
!! AAATAATAATCTATAAAAGA+TGG - chr1_4:15160315-15160334 None:intergenic 10.0%
!! AAATAATAATCTATAAAAGA+TGG - chr1_4:15160315-15160334 None:intergenic 10.0%
!! AATAATATAGTGTATAATTA+TGG + chr1_4:15159100-15159119 Msa0152480:intron 10.0%
!! ATATTAATATATACACAAAA+AGG + chr1_4:15159177-15159196 Msa0152480:intron 10.0%
!! TATTAATATATACACAAAAA+GGG + chr1_4:15159178-15159197 Msa0152480:intron 10.0%
!! ATAGATATATAAATTCTTTA+CGG + chr1_4:15159777-15159796 Msa0152480:intron 10.0%
!! AAATAATAATCTATAAAAGA+TGG - chr1_4:15160315-15160334 None:intergenic 10.0%
!! AAATAATAATCTATAAAAGA+TGG - chr1_4:15160315-15160334 None:intergenic 10.0%
!! ACAACTTAAATTTATTTAAG+AGG + chr1_4:15158999-15159018 Msa0152480:intron 15.0%
!! GTATTACATACATTAATTAA+TGG + chr1_4:15159573-15159592 Msa0152480:intron 15.0%
!!! TTGTTACTTAGTAATTAATT+TGG - chr1_4:15159814-15159833 None:intergenic 15.0%
!!! TTACTAAGTAACAATTTTTA+TGG + chr1_4:15159820-15159839 Msa0152480:intron 15.0%
!! ACAACTTAAATTTATTTAAG+AGG + chr1_4:15158999-15159018 Msa0152480:intron 15.0%
!! GTATTACATACATTAATTAA+TGG + chr1_4:15159573-15159592 Msa0152480:intron 15.0%
!!! TTGTTACTTAGTAATTAATT+TGG - chr1_4:15159814-15159833 None:intergenic 15.0%
!!! TTACTAAGTAACAATTTTTA+TGG + chr1_4:15159820-15159839 Msa0152480:intron 15.0%
!! TAATTATGGAAAGATAGTTT+AGG + chr1_4:15159114-15159133 Msa0152480:intron 20.0%
!!! TCTTTTTTGCAAAAGAAAAA+TGG + chr1_4:15159362-15159381 Msa0152480:intron 20.0%
!!! AAGAAAAATGGATTAATCAA+AGG + chr1_4:15159374-15159393 Msa0152480:intron 20.0%
!!! AGTAATTAATTTGGATGTTT+TGG - chr1_4:15159805-15159824 None:intergenic 20.0%
!! AATCATATTATATGTGAACT+AGG - chr1_4:15160681-15160700 None:intergenic 20.0%
!! TATGATTCATTTATGTACAT+AGG + chr1_4:15160694-15160713 Msa0152480:intron 20.0%
!! TAATTATGGAAAGATAGTTT+AGG + chr1_4:15159114-15159133 Msa0152480:intron 20.0%
!!! TCTTTTTTGCAAAAGAAAAA+TGG + chr1_4:15159362-15159381 Msa0152480:intron 20.0%
!!! AAGAAAAATGGATTAATCAA+AGG + chr1_4:15159374-15159393 Msa0152480:intron 20.0%
!!! AGTAATTAATTTGGATGTTT+TGG - chr1_4:15159805-15159824 None:intergenic 20.0%
!! AATCATATTATATGTGAACT+AGG - chr1_4:15160681-15160700 None:intergenic 20.0%
!! TATGATTCATTTATGTACAT+AGG + chr1_4:15160694-15160713 Msa0152480:intron 20.0%
! AAAAGAAAGTGAAAATGAAG+AGG + chr1_4:15158881-15158900 Msa0152480:CDS 25.0%
! TAATTTCATTCCATACATGT+TGG - chr1_4:15159406-15159425 None:intergenic 25.0%
!! TCTTCATTCCTTTATTTCTT+AGG + chr1_4:15159434-15159453 Msa0152480:intron 25.0%
! CATACATTAATTAATGGTCA+TGG + chr1_4:15159579-15159598 Msa0152480:intron 25.0%
! TGTGTGTAAAATAGATATTG+TGG + chr1_4:15159733-15159752 Msa0152480:intron 25.0%
! TCATGAAAGTTGTCTATTAA+TGG + chr1_4:15159998-15160017 Msa0152480:intron 25.0%
!!! ACGTAAATTGAAAGTTTTGA+AGG - chr1_4:15160067-15160086 None:intergenic 25.0%
! GTAACTAAAACTTGTCATTA+TGG - chr1_4:15160092-15160111 None:intergenic 25.0%
!!! TTTTTAAGCAAGCATAAACT+AGG + chr1_4:15160575-15160594 Msa0152480:intron 25.0%
! AAAAGAAAGTGAAAATGAAG+AGG + chr1_4:15158881-15158900 Msa0152480:CDS 25.0%
! TAATTTCATTCCATACATGT+TGG - chr1_4:15159406-15159425 None:intergenic 25.0%
!! TCTTCATTCCTTTATTTCTT+AGG + chr1_4:15159434-15159453 Msa0152480:intron 25.0%
! CATACATTAATTAATGGTCA+TGG + chr1_4:15159579-15159598 Msa0152480:intron 25.0%
! TGTGTGTAAAATAGATATTG+TGG + chr1_4:15159733-15159752 Msa0152480:intron 25.0%
! TCATGAAAGTTGTCTATTAA+TGG + chr1_4:15159998-15160017 Msa0152480:intron 25.0%
!!! ACGTAAATTGAAAGTTTTGA+AGG - chr1_4:15160067-15160086 None:intergenic 25.0%
! GTAACTAAAACTTGTCATTA+TGG - chr1_4:15160092-15160111 None:intergenic 25.0%
!!! TTTTTAAGCAAGCATAAACT+AGG + chr1_4:15160575-15160594 Msa0152480:intron 25.0%
TGTAAGTAATGTATTCCTCA+TGG - chr1_4:15159150-15159169 None:intergenic 30.0%
AGGGTAAAAAAGACAACAAA+AGG + chr1_4:15159197-15159216 Msa0152480:intron 30.0%
GGGTAAAAAAGACAACAAAA+GGG + chr1_4:15159198-15159217 Msa0152480:intron 30.0%
AAGGATATATCCAACATGTA+TGG + chr1_4:15159393-15159412 Msa0152480:intron 30.0%
! CATTCCTTTATTTCTTAGGT+CGG + chr1_4:15159438-15159457 Msa0152480:intron 30.0%
TTGATATAACAAGTGATGCA+TGG + chr1_4:15159481-15159500 Msa0152480:CDS 30.0%
! AATCAAATTTGTTCGCTCAA+TGG + chr1_4:15159685-15159704 Msa0152480:intron 30.0%
GTCAAAAAGTTGTTGCAAAA+TGG - chr1_4:15159880-15159899 None:intergenic 30.0%
! AAAGCTTTTGCTTCTTTTGT+TGG - chr1_4:15160767-15160786 None:intergenic 30.0%
TGTAAGTAATGTATTCCTCA+TGG - chr1_4:15159150-15159169 None:intergenic 30.0%
AGGGTAAAAAAGACAACAAA+AGG + chr1_4:15159197-15159216 Msa0152480:intron 30.0%
GGGTAAAAAAGACAACAAAA+GGG + chr1_4:15159198-15159217 Msa0152480:intron 30.0%
AAGGATATATCCAACATGTA+TGG + chr1_4:15159393-15159412 Msa0152480:intron 30.0%
! CATTCCTTTATTTCTTAGGT+CGG + chr1_4:15159438-15159457 Msa0152480:intron 30.0%
TTGATATAACAAGTGATGCA+TGG + chr1_4:15159481-15159500 Msa0152480:CDS 30.0%
! AATCAAATTTGTTCGCTCAA+TGG + chr1_4:15159685-15159704 Msa0152480:intron 30.0%
GTCAAAAAGTTGTTGCAAAA+TGG - chr1_4:15159880-15159899 None:intergenic 30.0%
! AAAGCTTTTGCTTCTTTTGT+TGG - chr1_4:15160767-15160786 None:intergenic 30.0%
GAGGGTAAAGAAATTGGAAA+GGG + chr1_4:15158843-15158862 Msa0152480:CDS 35.0%
TCAACCAATGCTTCAAACAA+AGG - chr1_4:15158933-15158952 None:intergenic 35.0%
GTCTCACATCAATTGTTTGA+AGG + chr1_4:15158953-15158972 Msa0152480:CDS 35.0%
AGATCCGACCTAAGAAATAA+AGG - chr1_4:15159445-15159464 None:intergenic 35.0%
AAGTGATGCATGGAAGAAAT+TGG + chr1_4:15159491-15159510 Msa0152480:CDS 35.0%
!!! GTTTGGACTAGTTGTTGTTT+TGG - chr1_4:15159529-15159548 None:intergenic 35.0%
!! TGATCAAGCTCTGAATTGTT+TGG - chr1_4:15159546-15159565 None:intergenic 35.0%
!!! TACATAGGAAGCATTTTGCA+TGG + chr1_4:15160709-15160728 Msa0152480:intron 35.0%
TATATTCAACTGCAGGAACT+TGG + chr1_4:15160800-15160819 Msa0152480:CDS 35.0%
GAGGGTAAAGAAATTGGAAA+GGG + chr1_4:15158843-15158862 Msa0152480:CDS 35.0%
TCAACCAATGCTTCAAACAA+AGG - chr1_4:15158933-15158952 None:intergenic 35.0%
GTCTCACATCAATTGTTTGA+AGG + chr1_4:15158953-15158972 Msa0152480:CDS 35.0%
AGATCCGACCTAAGAAATAA+AGG - chr1_4:15159445-15159464 None:intergenic 35.0%
AAGTGATGCATGGAAGAAAT+TGG + chr1_4:15159491-15159510 Msa0152480:CDS 35.0%
!!! GTTTGGACTAGTTGTTGTTT+TGG - chr1_4:15159529-15159548 None:intergenic 35.0%
!! TGATCAAGCTCTGAATTGTT+TGG - chr1_4:15159546-15159565 None:intergenic 35.0%
!!! TACATAGGAAGCATTTTGCA+TGG + chr1_4:15160709-15160728 Msa0152480:intron 35.0%
TATATTCAACTGCAGGAACT+TGG + chr1_4:15160800-15160819 Msa0152480:CDS 35.0%
GGAGGGTAAAGAAATTGGAA+AGG + chr1_4:15158842-15158861 Msa0152480:CDS 40.0%
CCAATGCTTCAAACAAAGGA+TGG - chr1_4:15158929-15158948 None:intergenic 40.0%
! CCATCCTTTGTTTGAAGCAT+TGG + chr1_4:15158926-15158945 Msa0152480:CDS 40.0%
! GGAAAGATAGTTTAGGACCA+CGG + chr1_4:15159121-15159140 Msa0152480:intron 40.0%
TATTTCTTAGGTCGGATCTG+AGG + chr1_4:15159446-15159465 Msa0152480:intron 40.0%
TTCAGAGCTTGATCACTTCA+TGG + chr1_4:15159551-15159570 Msa0152480:CDS 40.0%
!! GTACGTATGGTTTTGCATGT+GGG - chr1_4:15160130-15160149 None:intergenic 40.0%
AATACGTTGTGACGTACGTA+TGG - chr1_4:15160143-15160162 None:intergenic 40.0%
AATACGTTGTGACGTACGTA+TGG - chr1_4:15160143-15160162 None:intergenic 40.0%
GTTCATCAATCACGTCTTTG+AGG - chr1_4:15160745-15160764 None:intergenic 40.0%
TGCTACGTATATTCAACTGC+AGG + chr1_4:15160793-15160812 Msa0152480:CDS 40.0%
GGAGGGTAAAGAAATTGGAA+AGG + chr1_4:15158842-15158861 Msa0152480:CDS 40.0%
CCAATGCTTCAAACAAAGGA+TGG - chr1_4:15158929-15158948 None:intergenic 40.0%
! CCATCCTTTGTTTGAAGCAT+TGG + chr1_4:15158926-15158945 Msa0152480:CDS 40.0%
! GGAAAGATAGTTTAGGACCA+CGG + chr1_4:15159121-15159140 Msa0152480:intron 40.0%
TATTTCTTAGGTCGGATCTG+AGG + chr1_4:15159446-15159465 Msa0152480:intron 40.0%
TTCAGAGCTTGATCACTTCA+TGG + chr1_4:15159551-15159570 Msa0152480:CDS 40.0%
!! GTACGTATGGTTTTGCATGT+GGG - chr1_4:15160130-15160149 None:intergenic 40.0%
AATACGTTGTGACGTACGTA+TGG - chr1_4:15160143-15160162 None:intergenic 40.0%
AATACGTTGTGACGTACGTA+TGG - chr1_4:15160143-15160162 None:intergenic 40.0%
GTTCATCAATCACGTCTTTG+AGG - chr1_4:15160745-15160764 None:intergenic 40.0%
TGCTACGTATATTCAACTGC+AGG + chr1_4:15160793-15160812 Msa0152480:CDS 40.0%
! GGAAAGGGAAGTGATGATGT+AGG + chr1_4:15158858-15158877 Msa0152480:CDS 45.0%
!! TGGTTTTGCATGTGGGTTGA+GGG - chr1_4:15160123-15160142 None:intergenic 45.0%
!! ATGGTTTTGCATGTGGGTTG+AGG - chr1_4:15160124-15160143 None:intergenic 45.0%
!! CGTACGTATGGTTTTGCATG+TGG - chr1_4:15160131-15160150 None:intergenic 45.0%
! GGAAAGGGAAGTGATGATGT+AGG + chr1_4:15158858-15158877 Msa0152480:CDS 45.0%
!! TGGTTTTGCATGTGGGTTGA+GGG - chr1_4:15160123-15160142 None:intergenic 45.0%
!! ATGGTTTTGCATGTGGGTTG+AGG - chr1_4:15160124-15160143 None:intergenic 45.0%
!! CGTACGTATGGTTTTGCATG+TGG - chr1_4:15160131-15160150 None:intergenic 45.0%
TGTATTCCTCATGGTGACCG+TGG - chr1_4:15159141-15159160 None:intergenic 50.0%
! TTGCATGTGGGTTGAGGGTT+CGG - chr1_4:15160118-15160137 None:intergenic 50.0%
! GACGTACGTATGGTTCTGCT+AGG - chr1_4:15160364-15160383 None:intergenic 50.0%
! CAACTGCAGGAACTTGGTGA+AGG + chr1_4:15160806-15160825 Msa0152480:CDS 50.0%
TGTATTCCTCATGGTGACCG+TGG - chr1_4:15159141-15159160 None:intergenic 50.0%
! TTGCATGTGGGTTGAGGGTT+CGG - chr1_4:15160118-15160137 None:intergenic 50.0%
! GACGTACGTATGGTTCTGCT+AGG - chr1_4:15160364-15160383 None:intergenic 50.0%
! CAACTGCAGGAACTTGGTGA+AGG + chr1_4:15160806-15160825 Msa0152480:CDS 50.0%
TTAGGACCACGGTCACCATG+AGG + chr1_4:15159132-15159151 Msa0152480:intron 55.0%
TTAGGACCACGGTCACCATG+AGG + chr1_4:15159132-15159151 Msa0152480:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 15158840 15160838 15158840 ID=Msa0152480;Name=Msa0152480
chr1_4 mRNA 15158840 15160838 15158840 ID=Msa0152480-mRNA-1;Parent=Msa0152480;Name=Msa0152480-mRNA-1;_AED=0.03;_eAED=0.04;_QI=0|0|0|1|1|1|3|0|124
chr1_4 exon 15158840 15158974 15158840 ID=Msa0152480-mRNA-1:exon:5956;Parent=Msa0152480-mRNA-1
chr1_4 exon 15159456 15159572 15159456 ID=Msa0152480-mRNA-1:exon:5957;Parent=Msa0152480-mRNA-1
chr1_4 exon 15160716 15160838 15160716 ID=Msa0152480-mRNA-1:exon:5958;Parent=Msa0152480-mRNA-1
chr1_4 CDS 15158840 15158974 15158840 ID=Msa0152480-mRNA-1:cds;Parent=Msa0152480-mRNA-1
chr1_4 CDS 15159456 15159572 15159456 ID=Msa0152480-mRNA-1:cds;Parent=Msa0152480-mRNA-1
chr1_4 CDS 15160716 15160838 15160716 ID=Msa0152480-mRNA-1:cds;Parent=Msa0152480-mRNA-1
Gene Sequence

>Msa0152480

ATGGAGGGTAAAGAAATTGGAAAGGGAAGTGATGATGTAGGAAAAGAAAGTGAAAATGAAGAGGTTCTAAAGAAGATTATTGCAAGCCATCCTTTGTTTGAAGCATTGGTTGAGTCTCACATCAATTGTTTGAAGGTCGGATCTGAGGATGCAAAAGAGTTTGATATAACAAGTGATGCATGGAAGAAATTGGTCAACACAAAGTCCAAAACAACAACTAGTCCAAACAATTCAGAGCTTGATCACTTCATGGAAGCATTTTGCATGGCACTGAGCAACCTCAAAGACGTGATTGATGAACCAACAAAAGAAGCAAAAGCTTTCATCAATGCTACGTATATTCAACTGCAGGAACTTGGTGAAGGAAATAATTAA

Protein sequence

>Msa0152480

MEGKEIGKGSDDVGKESENEEVLKKIIASHPLFEALVESHINCLKVGSEDAKEFDITSDAWKKLVNTKSKTTTSPNNSELDHFMEAFCMALSNLKDVIDEPTKEAKAFINATYIQLQELGEGNN*