Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459530 | XP_003625674.1 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.26e-39 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459530 | sp|Q7XXN8|DVL16_ARATH | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.33e-19 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459530 | G7L3V3 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.56e-39 | 133 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0019600 | Msa1459530 | 0.824901 | 6.279313e-54 | -8.615850e-47 |
| Msa0050040 | Msa1459530 | 0.806074 | 9.815564e-50 | -8.615850e-47 |
| Msa0055450 | Msa1459530 | 0.800584 | 1.345940e-48 | -8.615850e-47 |
| Msa0084500 | Msa1459530 | 0.815743 | 7.905004e-52 | -8.615850e-47 |
| Msa0102990 | Msa1459530 | 0.807707 | 4.431525e-50 | -8.615850e-47 |
| Msa1374550 | Msa1459530 | 0.809032 | 2.312462e-50 | -8.615850e-47 |
| Msa1379970 | Msa1459530 | 0.852933 | 3.335119e-61 | -8.615850e-47 |
| Msa1394890 | Msa1459530 | 0.991708 | 4.168767e-189 | -8.615850e-47 |
| Msa1406780 | Msa1459530 | 0.835291 | 1.827860e-56 | -8.615850e-47 |
| Msa1420060 | Msa1459530 | 0.821949 | 3.075783e-53 | -8.615850e-47 |
| Msa1421550 | Msa1459530 | 0.863486 | 2.405061e-64 | -8.615850e-47 |
| Msa1423460 | Msa1459530 | 0.823068 | 1.689793e-53 | -8.615850e-47 |
| Msa1431850 | Msa1459530 | 0.840386 | 8.972051e-58 | -8.615850e-47 |
| Msa1435480 | Msa1459530 | 0.813175 | 2.923395e-51 | -8.615850e-47 |
| Msa1455320 | Msa1459530 | 0.816501 | 5.352732e-52 | -8.615850e-47 |
| Msa1459530 | Msa1462530 | 0.825747 | 3.959601e-54 | -8.615850e-47 |
| Msa0669840 | Msa1459530 | 0.872699 | 2.601184e-67 | -8.615850e-47 |
| Msa0711320 | Msa1459530 | 0.818534 | 1.864781e-52 | -8.615850e-47 |
| Msa0739580 | Msa1459530 | 0.844504 | 7.255755e-59 | -8.615850e-47 |
| Msa0770290 | Msa1459530 | 0.824500 | 7.803148e-54 | -8.615850e-47 |
| Msa0782360 | Msa1459530 | 0.842873 | 1.982115e-58 | -8.615850e-47 |
| Msa0786040 | Msa1459530 | 0.830328 | 3.123659e-55 | -8.615850e-47 |
| Msa0819890 | Msa1459530 | 0.810801 | 9.619979e-51 | -8.615850e-47 |
| Msa0823400 | Msa1459530 | 0.823448 | 1.377600e-53 | -8.615850e-47 |
| Msa0124130 | Msa1459530 | 0.811993 | 5.302992e-51 | -8.615850e-47 |
| Msa0152480 | Msa1459530 | 0.806038 | 9.989256e-50 | -8.615850e-47 |
| Msa0152500 | Msa1459530 | 0.816310 | 5.905718e-52 | -8.615850e-47 |
| Msa0156250 | Msa1459530 | 0.820239 | 7.618291e-53 | -8.615850e-47 |
| Msa0185490 | Msa1459530 | 0.819849 | 9.359710e-53 | -8.615850e-47 |
| Msa0203030 | Msa1459530 | 0.800101 | 1.687927e-48 | -8.615850e-47 |
| Msa0212080 | Msa1459530 | 0.802265 | 6.089993e-49 | -8.615850e-47 |
| Msa0212150 | Msa1459530 | 0.841682 | 4.097989e-58 | -8.615850e-47 |
| Msa0215640 | Msa1459530 | 0.815361 | 9.618250e-52 | -8.615850e-47 |
| Msa0219190 | Msa1459530 | 0.800974 | 1.120507e-48 | -8.615850e-47 |
| Msa0221290 | Msa1459530 | 0.800744 | 1.248189e-48 | -8.615850e-47 |
| Msa1228190 | Msa1459530 | 0.857699 | 1.366284e-62 | -8.615850e-47 |
| Msa1233560 | Msa1459530 | 0.824537 | 7.651715e-54 | -8.615850e-47 |
| Msa1271260 | Msa1459530 | 0.834639 | 2.668740e-56 | -8.615850e-47 |
| Msa1294290 | Msa1459530 | 0.823578 | 1.284690e-53 | -8.615850e-47 |
| Msa1305510 | Msa1459530 | 0.808806 | 2.584460e-50 | -8.615850e-47 |
| Msa1313970 | Msa1459530 | 0.808449 | 3.080132e-50 | -8.615850e-47 |
| Msa1326630 | Msa1459530 | 0.804412 | 2.187400e-49 | -8.615850e-47 |
| Msa0375680 | Msa1459530 | 0.804703 | 1.901947e-49 | -8.615850e-47 |
| Msa0384910 | Msa1459530 | 0.807915 | 4.003509e-50 | -8.615850e-47 |
| Msa0385390 | Msa1459530 | 0.810398 | 1.175597e-50 | -8.615850e-47 |
| Msa0392420 | Msa1459530 | 0.812763 | 3.600295e-51 | -8.615850e-47 |
| Msa0392460 | Msa1459530 | 0.810632 | 1.046733e-50 | -8.615850e-47 |
| Msa0392490 | Msa1459530 | 0.813894 | 2.030929e-51 | -8.615850e-47 |
| Msa0406000 | Msa1459530 | 0.855989 | 4.354720e-62 | -8.615850e-47 |
| Msa0424330 | Msa1459530 | 0.838166 | 3.379938e-57 | -8.615850e-47 |
| Msa0424340 | Msa1459530 | 0.811080 | 8.373243e-51 | -8.615850e-47 |
| Msa0424350 | Msa1459530 | 0.830364 | 3.061951e-55 | -8.615850e-47 |
| Msa0441590 | Msa1459530 | 0.813724 | 2.213896e-51 | -8.615850e-47 |
| Msa0451400 | Msa1459530 | 0.874166 | 8.353642e-68 | -8.615850e-47 |
| Msa0999300 | Msa1459530 | 0.851633 | 7.816155e-61 | -8.615850e-47 |
| Msa0999360 | Msa1459530 | 0.818287 | 2.120515e-52 | -8.615850e-47 |
| Msa0999410 | Msa1459530 | 0.806925 | 6.490449e-50 | -8.615850e-47 |
| Msa1001890 | Msa1459530 | 0.840316 | 9.360379e-58 | -8.615850e-47 |
| Msa1037770 | Msa1459530 | 0.804985 | 1.660785e-49 | -8.615850e-47 |
| Msa1037890 | Msa1459530 | 0.831240 | 1.867394e-55 | -8.615850e-47 |
| Msa1037900 | Msa1459530 | 0.815928 | 7.189242e-52 | -8.615850e-47 |
| Msa1037910 | Msa1459530 | 0.811654 | 6.284897e-51 | -8.615850e-47 |
| Msa1047990 | Msa1459530 | 0.807374 | 5.214692e-50 | -8.615850e-47 |
| Msa1081570 | Msa1459530 | 0.808257 | 3.384265e-50 | -8.615850e-47 |
| Msa1084520 | Msa1459530 | 0.830983 | 2.159709e-55 | -8.615850e-47 |
| Msa1092020 | Msa1459530 | 0.825608 | 4.272590e-54 | -8.615850e-47 |
| Msa1096210 | Msa1459530 | 0.801027 | 1.092683e-48 | -8.615850e-47 |
| Msa0252630 | Msa1459530 | 0.803697 | 3.080331e-49 | -8.615850e-47 |
| Msa0252770 | Msa1459530 | 0.859693 | 3.467432e-63 | -8.615850e-47 |
| Msa0289920 | Msa1459530 | 0.815647 | 8.306784e-52 | -8.615850e-47 |
| Msa0298240 | Msa1459530 | 0.822025 | 2.954377e-53 | -8.615850e-47 |
| Msa0306120 | Msa1459530 | 0.842622 | 2.311325e-58 | -8.615850e-47 |
| Msa0322840 | Msa1459530 | 0.819177 | 1.331499e-52 | -8.615850e-47 |
| Msa0329620 | Msa1459530 | 0.857686 | 1.378727e-62 | -8.615850e-47 |
| Msa0333700 | Msa1459530 | 0.815837 | 7.532793e-52 | -8.615850e-47 |
| Msa0344570 | Msa1459530 | 0.807154 | 5.805817e-50 | -8.615850e-47 |
| Msa0359250 | Msa1459530 | 0.872016 | 4.393836e-67 | -8.615850e-47 |
| Msa1125810 | Msa1459530 | 0.802242 | 6.155574e-49 | -8.615850e-47 |
| Msa1127140 | Msa1459530 | 0.813891 | 2.034970e-51 | -8.615850e-47 |
| Msa1129950 | Msa1459530 | 0.815795 | 7.697448e-52 | -8.615850e-47 |
| Msa1129960 | Msa1459530 | 0.817769 | 2.776426e-52 | -8.615850e-47 |
| Msa1129970 | Msa1459530 | 0.813200 | 2.886331e-51 | -8.615850e-47 |
| Msa1130010 | Msa1459530 | 0.808387 | 3.176033e-50 | -8.615850e-47 |
| Msa1135380 | Msa1459530 | 0.839096 | 1.943689e-57 | -8.615850e-47 |
| Msa1145020 | Msa1459530 | 0.835621 | 1.507926e-56 | -8.615850e-47 |
| Msa1166400 | Msa1459530 | 0.820247 | 7.587478e-53 | -8.615850e-47 |
| Msa1171310 | Msa1459530 | 0.802915 | 4.472724e-49 | -8.615850e-47 |
| Msa1201110 | Msa1459530 | 0.815283 | 1.000983e-51 | -8.615850e-47 |
| Msa1201160 | Msa1459530 | 0.833290 | 5.804608e-56 | -8.615850e-47 |
| Msa0487570 | Msa1459530 | 0.818254 | 2.157165e-52 | -8.615850e-47 |
| Msa0487630 | Msa1459530 | 0.821907 | 3.144860e-53 | -8.615850e-47 |
| Msa0492190 | Msa1459530 | 0.841175 | 5.570802e-58 | -8.615850e-47 |
| Msa0515950 | Msa1459530 | 0.812915 | 3.334401e-51 | -8.615850e-47 |
| Msa0534930 | Msa1459530 | 0.846566 | 2.004380e-59 | -8.615850e-47 |
| Msa0535040 | Msa1459530 | 0.842874 | 1.980668e-58 | -8.615850e-47 |
| Msa0575740 | Msa1459530 | 0.812997 | 3.199428e-51 | -8.615850e-47 |
| Msa0591790 | Msa1459530 | 0.855034 | 8.270829e-62 | -8.615850e-47 |
| Msa0594090 | Msa1459530 | 0.809706 | 1.657246e-50 | -8.615850e-47 |
| Msa0849280 | Msa1459530 | 0.820073 | 8.316640e-53 | -8.615850e-47 |
| Msa0851020 | Msa1459530 | 0.823576 | 1.285971e-53 | -8.615850e-47 |
| Msa0860160 | Msa1459530 | 0.810183 | 1.308336e-50 | -8.615850e-47 |
| Msa0860280 | Msa1459530 | 0.856253 | 3.644550e-62 | -8.615850e-47 |
| Msa0867350 | Msa1459530 | 0.835942 | 1.250989e-56 | -8.615850e-47 |
| Msa0885060 | Msa1459530 | 0.804878 | 1.748195e-49 | -8.615850e-47 |
| Msa0898730 | Msa1459530 | 0.824140 | 9.483676e-54 | -8.615850e-47 |
| Msa0929870 | Msa1459530 | 0.851871 | 6.689508e-61 | -8.615850e-47 |
| Msa0936410 | Msa1459530 | 0.821521 | 3.863341e-53 | -8.615850e-47 |
| Msa0944760 | Msa1459530 | 0.821005 | 5.081851e-53 | -8.615850e-47 |
| Msa0961560 | Msa1459530 | 0.806381 | 8.456014e-50 | -8.615850e-47 |
| Msa0973250 | Msa1459530 | 0.826608 | 2.471661e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459530 | MtrunA17_Chr7g0264501 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.00e-43 | 133 |
| Msa1459530 | MtrunA17_Chr1g0180861 | 57.143 | 63 | 26 | 1 | 1 | 62 | 3 | 65 | 1.83e-20 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1459530 | AT2G36985.1 | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.42e-20 | 75.1 |
| Msa1459530 | AT3G53232.1 | 70.588 | 34 | 10 | 0 | 24 | 57 | 11 | 44 | 1.21e-12 | 56.6 |
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGCTGATGAGTACTATTT+TGG | 0.208088 | tig0040311:+13607 | Msa1459530:intergenic |
| TGACCAAGTGACTACACTTT+TGG | 0.325622 | tig0040311:+13547 | Msa1459530:intergenic |
| TGTGATCCGTGCAAGTCTTT+TGG | 0.334940 | tig0040311:-13570 | Msa1459530:CDS |
| AAAATCATTCATGGCAGTAA+AGG | 0.489511 | tig0040311:-13649 | None:intergenic |
| TGGCCAAAAGTGTAGTCACT+TGG | 0.560139 | tig0040311:-13550 | Msa1459530:CDS |
| CATCAGCATCAAGAACAAGA+AGG | 0.581729 | tig0040311:-13594 | Msa1459530:CDS |
| ACAAAAGCATGGCGATGCAA+CGG | 0.595123 | tig0040311:+13493 | Msa1459530:intergenic |
| ATCAGCATCAAGAACAAGAA+GGG | 0.607865 | tig0040311:-13593 | Msa1459530:CDS |
| TTCATGATGACACAAAAGCA+TGG | 0.650703 | tig0040311:+13482 | Msa1459530:intergenic |
| TAAAGGAAATTAGCAGCACA+AGG | 0.665511 | tig0040311:-13632 | Msa1459530:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAAGGAAATTAGCAGCACA+AGG | - | tig0040311:13474-13493 | Msa1459530:CDS | 35.0% | |
| ! | GATGCTGATGAGTACTATTT+TGG | + | tig0040311:13502-13521 | Msa1459530:intergenic | 35.0% |
| ATCAGCATCAAGAACAAGAA+GGG | - | tig0040311:13513-13532 | Msa1459530:CDS | 35.0% | |
| TTCATGATGACACAAAAGCA+TGG | + | tig0040311:13627-13646 | Msa1459530:intergenic | 35.0% | |
| CATCAGCATCAAGAACAAGA+AGG | - | tig0040311:13512-13531 | Msa1459530:CDS | 40.0% | |
| ! | TTTTGGCCAAAAGACTTGCA+CGG | + | tig0040311:13545-13564 | Msa1459530:intergenic | 40.0% |
| ! | TGACCAAGTGACTACACTTT+TGG | + | tig0040311:13562-13581 | Msa1459530:intergenic | 40.0% |
| ! | TGTGATCCGTGCAAGTCTTT+TGG | - | tig0040311:13536-13555 | Msa1459530:CDS | 45.0% |
| ! | TGGCCAAAAGTGTAGTCACT+TGG | - | tig0040311:13556-13575 | Msa1459530:CDS | 45.0% |
| ! | ACAAAAGCATGGCGATGCAA+CGG | + | tig0040311:13616-13635 | Msa1459530:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0040311 | gene | 13467 | 13661 | 13467 | ID=Msa1459530;Name=Msa1459530 |
| tig0040311 | mRNA | 13467 | 13661 | 13467 | ID=Msa1459530-mRNA-1;Parent=Msa1459530;Name=Msa1459530-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|64 |
| tig0040311 | exon | 13467 | 13661 | 13467 | ID=Msa1459530-mRNA-1:exon:22290;Parent=Msa1459530-mRNA-1 |
| tig0040311 | CDS | 13467 | 13661 | 13467 | ID=Msa1459530-mRNA-1:cds;Parent=Msa1459530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
