Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394890 | XP_003625674.1 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.26e-39 | 133 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394890 | sp|Q7XXN8|DVL16_ARATH | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.33e-19 | 75.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394890 | G7L3V3 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 1.56e-39 | 133 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0016120 | Msa1394890 | 0.803053 | 4.187437e-49 | -8.615850e-47 |
| Msa0019600 | Msa1394890 | 0.839734 | 1.326933e-57 | -8.615850e-47 |
| Msa0050040 | Msa1394890 | 0.812784 | 3.561357e-51 | -8.615850e-47 |
| Msa0055450 | Msa1394890 | 0.809368 | 1.958658e-50 | -8.615850e-47 |
| Msa0084500 | Msa1394890 | 0.816327 | 5.854024e-52 | -8.615850e-47 |
| Msa0098440 | Msa1394890 | 0.802292 | 6.012674e-49 | -8.615850e-47 |
| Msa0102990 | Msa1394890 | 0.814194 | 1.744196e-51 | -8.615850e-47 |
| Msa1374550 | Msa1394890 | 0.819687 | 1.018970e-52 | -8.615850e-47 |
| Msa1379970 | Msa1394890 | 0.854919 | 8.929701e-62 | -8.615850e-47 |
| Msa1394890 | Msa1406780 | 0.851490 | 8.579852e-61 | -8.615850e-47 |
| Msa1394890 | Msa1420060 | 0.830850 | 2.327826e-55 | -8.615850e-47 |
| Msa1394890 | Msa1421550 | 0.871920 | 4.728632e-67 | -8.615850e-47 |
| Msa1394890 | Msa1423460 | 0.834921 | 2.265672e-56 | -8.615850e-47 |
| Msa1394890 | Msa1431850 | 0.857619 | 1.443240e-62 | -8.615850e-47 |
| Msa1394890 | Msa1435480 | 0.806388 | 8.426219e-50 | -8.615850e-47 |
| Msa1394890 | Msa1455320 | 0.824781 | 6.699764e-54 | -8.615850e-47 |
| Msa1394890 | Msa1459530 | 0.991708 | 4.168767e-189 | -8.615850e-47 |
| Msa1394890 | Msa1461060 | 0.818602 | 1.799120e-52 | -8.615850e-47 |
| Msa1394890 | Msa1462530 | 0.825963 | 3.518985e-54 | -8.615850e-47 |
| Msa0641250 | Msa1394890 | 0.810630 | 1.047918e-50 | -8.615850e-47 |
| Msa0669840 | Msa1394890 | 0.882578 | 9.264523e-71 | -8.615850e-47 |
| Msa0711320 | Msa1394890 | 0.833503 | 5.138118e-56 | -8.615850e-47 |
| Msa0739580 | Msa1394890 | 0.861495 | 9.854750e-64 | -8.615850e-47 |
| Msa0748400 | Msa1394890 | 0.800657 | 1.300201e-48 | -8.615850e-47 |
| Msa0752840 | Msa1394890 | 0.814261 | 1.685439e-51 | -8.615850e-47 |
| Msa0770290 | Msa1394890 | 0.836090 | 1.147092e-56 | -8.615850e-47 |
| Msa0782360 | Msa1394890 | 0.858411 | 8.394989e-63 | -8.615850e-47 |
| Msa0786040 | Msa1394890 | 0.830000 | 3.757088e-55 | -8.615850e-47 |
| Msa0819890 | Msa1394890 | 0.823442 | 1.382093e-53 | -8.615850e-47 |
| Msa0823400 | Msa1394890 | 0.818427 | 1.971511e-52 | -8.615850e-47 |
| Msa0831940 | Msa1394890 | 0.805486 | 1.304562e-49 | -8.615850e-47 |
| Msa0124130 | Msa1394890 | 0.828643 | 8.021875e-55 | -8.615850e-47 |
| Msa0141120 | Msa1394890 | 0.813294 | 2.752452e-51 | -8.615850e-47 |
| Msa0149140 | Msa1394890 | 0.814003 | 1.922396e-51 | -8.615850e-47 |
| Msa0152480 | Msa1394890 | 0.824799 | 6.635168e-54 | -8.615850e-47 |
| Msa0152500 | Msa1394890 | 0.835201 | 1.925964e-56 | -8.615850e-47 |
| Msa0156250 | Msa1394890 | 0.832307 | 1.018618e-55 | -8.615850e-47 |
| Msa0185490 | Msa1394890 | 0.832341 | 9.989347e-56 | -8.615850e-47 |
| Msa0197100 | Msa1394890 | 0.805248 | 1.462818e-49 | -8.615850e-47 |
| Msa0203030 | Msa1394890 | 0.802274 | 6.064255e-49 | -8.615850e-47 |
| Msa0212080 | Msa1394890 | 0.815064 | 1.119320e-51 | -8.615850e-47 |
| Msa0212150 | Msa1394890 | 0.854757 | 9.951259e-62 | -8.615850e-47 |
| Msa0215640 | Msa1394890 | 0.830228 | 3.305883e-55 | -8.615850e-47 |
| Msa0219190 | Msa1394890 | 0.809962 | 1.459798e-50 | -8.615850e-47 |
| Msa0221290 | Msa1394890 | 0.816347 | 5.795782e-52 | -8.615850e-47 |
| Msa0232920 | Msa1394890 | 0.805756 | 1.144532e-49 | -8.615850e-47 |
| Msa1228190 | Msa1394890 | 0.866453 | 2.820651e-65 | -8.615850e-47 |
| Msa1233560 | Msa1394890 | 0.831603 | 1.519875e-55 | -8.615850e-47 |
| Msa1271260 | Msa1394890 | 0.842294 | 2.823019e-58 | -8.615850e-47 |
| Msa1294290 | Msa1394890 | 0.833966 | 3.936527e-56 | -8.615850e-47 |
| Msa1305510 | Msa1394890 | 0.829171 | 5.976631e-55 | -8.615850e-47 |
| Msa1307190 | Msa1394890 | 0.800831 | 1.198557e-48 | -8.615850e-47 |
| Msa1313970 | Msa1394890 | 0.814990 | 1.162780e-51 | -8.615850e-47 |
| Msa1326630 | Msa1394890 | 0.814698 | 1.349458e-51 | -8.615850e-47 |
| Msa0375680 | Msa1394890 | 0.820390 | 7.034444e-53 | -8.615850e-47 |
| Msa0384910 | Msa1394890 | 0.824469 | 7.936575e-54 | -8.615850e-47 |
| Msa0385390 | Msa1394890 | 0.816455 | 5.480455e-52 | -8.615850e-47 |
| Msa0392420 | Msa1394890 | 0.821653 | 3.601061e-53 | -8.615850e-47 |
| Msa0392460 | Msa1394890 | 0.820483 | 6.696540e-53 | -8.615850e-47 |
| Msa0392490 | Msa1394890 | 0.822589 | 2.184452e-53 | -8.615850e-47 |
| Msa0406000 | Msa1394890 | 0.873167 | 1.813984e-67 | -8.615850e-47 |
| Msa0415320 | Msa1394890 | 0.804489 | 2.107442e-49 | -8.615850e-47 |
| Msa0424330 | Msa1394890 | 0.853199 | 2.798361e-61 | -8.615850e-47 |
| Msa0424340 | Msa1394890 | 0.827243 | 1.742223e-54 | -8.615850e-47 |
| Msa0424350 | Msa1394890 | 0.849468 | 3.171332e-60 | -8.615850e-47 |
| Msa0441590 | Msa1394890 | 0.825798 | 3.852235e-54 | -8.615850e-47 |
| Msa0451400 | Msa1394890 | 0.884769 | 1.444960e-71 | -8.615850e-47 |
| Msa0479540 | Msa1394890 | 0.805729 | 1.160052e-49 | -8.615850e-47 |
| Msa0999300 | Msa1394890 | 0.864740 | 9.780143e-65 | -8.615850e-47 |
| Msa0999360 | Msa1394890 | 0.829701 | 4.441560e-55 | -8.615850e-47 |
| Msa0999410 | Msa1394890 | 0.818442 | 1.955649e-52 | -8.615850e-47 |
| Msa1001890 | Msa1394890 | 0.850975 | 1.198975e-60 | -8.615850e-47 |
| Msa1037770 | Msa1394890 | 0.816841 | 4.490776e-52 | -8.615850e-47 |
| Msa1037890 | Msa1394890 | 0.842409 | 2.632317e-58 | -8.615850e-47 |
| Msa1037900 | Msa1394890 | 0.829744 | 4.336765e-55 | -8.615850e-47 |
| Msa1037910 | Msa1394890 | 0.823772 | 1.157001e-53 | -8.615850e-47 |
| Msa1041530 | Msa1394890 | 0.808696 | 2.728017e-50 | -8.615850e-47 |
| Msa1047990 | Msa1394890 | 0.807535 | 4.820184e-50 | -8.615850e-47 |
| Msa1081570 | Msa1394890 | 0.819524 | 1.110391e-52 | -8.615850e-47 |
| Msa1084520 | Msa1394890 | 0.830185 | 3.386070e-55 | -8.615850e-47 |
| Msa1092020 | Msa1394890 | 0.825661 | 4.151355e-54 | -8.615850e-47 |
| Msa1096210 | Msa1394890 | 0.814399 | 1.571121e-51 | -8.615850e-47 |
| Msa0239030 | Msa1394890 | 0.815138 | 1.077768e-51 | -8.615850e-47 |
| Msa0252630 | Msa1394890 | 0.814215 | 1.725752e-51 | -8.615850e-47 |
| Msa0252770 | Msa1394890 | 0.876017 | 1.950009e-68 | -8.615850e-47 |
| Msa0276740 | Msa1394890 | 0.800331 | 1.515024e-48 | -8.615850e-47 |
| Msa0289920 | Msa1394890 | 0.832417 | 9.564415e-56 | -8.615850e-47 |
| Msa0298240 | Msa1394890 | 0.831187 | 1.923960e-55 | -8.615850e-47 |
| Msa0306120 | Msa1394890 | 0.855894 | 4.644018e-62 | -8.615850e-47 |
| Msa0316680 | Msa1394890 | 0.806172 | 9.360980e-50 | -8.615850e-47 |
| Msa0322840 | Msa1394890 | 0.823507 | 1.334418e-53 | -8.615850e-47 |
| Msa0322860 | Msa1394890 | 0.801514 | 8.686946e-49 | -8.615850e-47 |
| Msa0329620 | Msa1394890 | 0.871688 | 5.649298e-67 | -8.615850e-47 |
| Msa0333700 | Msa1394890 | 0.815204 | 1.042157e-51 | -8.615850e-47 |
| Msa0343660 | Msa1394890 | 0.812779 | 3.570360e-51 | -8.615850e-47 |
| Msa0344570 | Msa1394890 | 0.817155 | 3.818740e-52 | -8.615850e-47 |
| Msa0351770 | Msa1394890 | 0.804637 | 1.963097e-49 | -8.615850e-47 |
| Msa0359250 | Msa1394890 | 0.885879 | 5.554100e-72 | -8.615850e-47 |
| Msa1125810 | Msa1394890 | 0.814348 | 1.612933e-51 | -8.615850e-47 |
| Msa1127140 | Msa1394890 | 0.827284 | 1.702867e-54 | -8.615850e-47 |
| Msa1129950 | Msa1394890 | 0.828392 | 9.225284e-55 | -8.615850e-47 |
| Msa1129960 | Msa1394890 | 0.831057 | 2.070728e-55 | -8.615850e-47 |
| Msa1129970 | Msa1394890 | 0.826459 | 2.681517e-54 | -8.615850e-47 |
| Msa1130010 | Msa1394890 | 0.822643 | 2.122333e-53 | -8.615850e-47 |
| Msa1135380 | Msa1394890 | 0.837741 | 4.345603e-57 | -8.615850e-47 |
| Msa1145020 | Msa1394890 | 0.840614 | 7.820700e-58 | -8.615850e-47 |
| Msa1166400 | Msa1394890 | 0.832799 | 7.692252e-56 | -8.615850e-47 |
| Msa1171310 | Msa1394890 | 0.815724 | 7.983981e-52 | -8.615850e-47 |
| Msa1176160 | Msa1394890 | 0.807212 | 5.644267e-50 | -8.615850e-47 |
| Msa1179280 | Msa1394890 | 0.803623 | 3.191549e-49 | -8.615850e-47 |
| Msa1201110 | Msa1394890 | 0.825308 | 5.032258e-54 | -8.615850e-47 |
| Msa1201160 | Msa1394890 | 0.843554 | 1.304954e-58 | -8.615850e-47 |
| Msa0487570 | Msa1394890 | 0.826579 | 2.510649e-54 | -8.615850e-47 |
| Msa0487630 | Msa1394890 | 0.830117 | 3.517902e-55 | -8.615850e-47 |
| Msa0492190 | Msa1394890 | 0.856575 | 2.932303e-62 | -8.615850e-47 |
| Msa0515950 | Msa1394890 | 0.827786 | 1.290377e-54 | -8.615850e-47 |
| Msa0534930 | Msa1394890 | 0.855234 | 7.234911e-62 | -8.615850e-47 |
| Msa0535040 | Msa1394890 | 0.850843 | 1.306791e-60 | -8.615850e-47 |
| Msa0575740 | Msa1394890 | 0.821003 | 5.086631e-53 | -8.615850e-47 |
| Msa0591790 | Msa1394890 | 0.863243 | 2.861297e-64 | -8.615850e-47 |
| Msa0594090 | Msa1394890 | 0.824957 | 6.089039e-54 | -8.615850e-47 |
| Msa0844560 | Msa1394890 | 0.802209 | 6.252600e-49 | -8.615850e-47 |
| Msa0849280 | Msa1394890 | 0.832436 | 9.463129e-56 | -8.615850e-47 |
| Msa0851020 | Msa1394890 | 0.836257 | 1.040300e-56 | -8.615850e-47 |
| Msa0860160 | Msa1394890 | 0.809914 | 1.495363e-50 | -8.615850e-47 |
| Msa0860280 | Msa1394890 | 0.871096 | 8.864079e-67 | -8.615850e-47 |
| Msa0867350 | Msa1394890 | 0.851716 | 7.403056e-61 | -8.615850e-47 |
| Msa0885060 | Msa1394890 | 0.813058 | 3.101755e-51 | -8.615850e-47 |
| Msa0896350 | Msa1394890 | 0.806660 | 7.384347e-50 | -8.615850e-47 |
| Msa0896970 | Msa1394890 | 0.802348 | 5.854206e-49 | -8.615850e-47 |
| Msa0898730 | Msa1394890 | 0.840305 | 9.419381e-58 | -8.615850e-47 |
| Msa0929870 | Msa1394890 | 0.860611 | 1.831250e-63 | -8.615850e-47 |
| Msa0936410 | Msa1394890 | 0.826683 | 2.370863e-54 | -8.615850e-47 |
| Msa0944760 | Msa1394890 | 0.832471 | 9.278386e-56 | -8.615850e-47 |
| Msa0944880 | Msa1394890 | 0.810803 | 9.610521e-51 | -8.615850e-47 |
| Msa0953540 | Msa1394890 | 0.808502 | 3.000898e-50 | -8.615850e-47 |
| Msa0961560 | Msa1394890 | 0.821665 | 3.578116e-53 | -8.615850e-47 |
| Msa0965310 | Msa1394890 | 0.805592 | 1.239049e-49 | -8.615850e-47 |
| Msa0973250 | Msa1394890 | 0.837188 | 6.024614e-57 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394890 | MtrunA17_Chr7g0264501 | 100.000 | 64 | 0 | 0 | 1 | 64 | 1 | 64 | 3.00e-43 | 133 |
| Msa1394890 | MtrunA17_Chr1g0180861 | 57.143 | 63 | 26 | 1 | 1 | 62 | 3 | 65 | 1.83e-20 | 76.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1394890 | AT2G36985.1 | 72.727 | 44 | 12 | 0 | 19 | 62 | 4 | 47 | 5.42e-20 | 75.1 |
| Msa1394890 | AT3G53232.1 | 70.588 | 34 | 10 | 0 | 24 | 57 | 11 | 44 | 1.21e-12 | 56.6 |
Find 10 sgRNAs with CRISPR-Local
Find 10 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGCTGATGAGTACTATTT+TGG | 0.208088 | tig0021901:+23818 | None:intergenic |
| TGACCAAGTGACTACACTTT+TGG | 0.325622 | tig0021901:+23758 | None:intergenic |
| TGTGATCCGTGCAAGTCTTT+TGG | 0.334940 | tig0021901:-23781 | Msa1394890:CDS |
| AAAATCATTCATGGCAGTAA+AGG | 0.489511 | tig0021901:-23860 | None:intergenic |
| TGGCCAAAAGTGTAGTCACT+TGG | 0.560139 | tig0021901:-23761 | Msa1394890:CDS |
| CATCAGCATCAAGAACAAGA+AGG | 0.581729 | tig0021901:-23805 | Msa1394890:CDS |
| ACAAAAGCATGGCGATGCAA+CGG | 0.595123 | tig0021901:+23704 | None:intergenic |
| ATCAGCATCAAGAACAAGAA+GGG | 0.607865 | tig0021901:-23804 | Msa1394890:CDS |
| TTCATGATGACACAAAAGCA+TGG | 0.650703 | tig0021901:+23693 | None:intergenic |
| TAAAGGAAATTAGCAGCACA+AGG | 0.665511 | tig0021901:-23843 | Msa1394890:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAAGGAAATTAGCAGCACA+AGG | - | tig0021901:23685-23704 | Msa1394890:CDS | 35.0% | |
| ! | GATGCTGATGAGTACTATTT+TGG | + | tig0021901:23713-23732 | None:intergenic | 35.0% |
| ATCAGCATCAAGAACAAGAA+GGG | - | tig0021901:23724-23743 | Msa1394890:CDS | 35.0% | |
| TTCATGATGACACAAAAGCA+TGG | + | tig0021901:23838-23857 | None:intergenic | 35.0% | |
| CATCAGCATCAAGAACAAGA+AGG | - | tig0021901:23723-23742 | Msa1394890:CDS | 40.0% | |
| ! | TTTTGGCCAAAAGACTTGCA+CGG | + | tig0021901:23756-23775 | None:intergenic | 40.0% |
| ! | TGACCAAGTGACTACACTTT+TGG | + | tig0021901:23773-23792 | None:intergenic | 40.0% |
| ! | TGTGATCCGTGCAAGTCTTT+TGG | - | tig0021901:23747-23766 | Msa1394890:CDS | 45.0% |
| ! | TGGCCAAAAGTGTAGTCACT+TGG | - | tig0021901:23767-23786 | Msa1394890:CDS | 45.0% |
| ! | ACAAAAGCATGGCGATGCAA+CGG | + | tig0021901:23827-23846 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021901 | gene | 23678 | 23872 | 23678 | ID=Msa1394890;Name=Msa1394890 |
| tig0021901 | mRNA | 23678 | 23872 | 23678 | ID=Msa1394890-mRNA-1;Parent=Msa1394890;Name=Msa1394890-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|-1|0|1|-1|1|1|0|64 |
| tig0021901 | exon | 23678 | 23872 | 23678 | ID=Msa1394890-mRNA-1:exon:14617;Parent=Msa1394890-mRNA-1 |
| tig0021901 | CDS | 23678 | 23872 | 23678 | ID=Msa1394890-mRNA-1:cds;Parent=Msa1394890-mRNA-1 |
| Gene Sequence |
| Protein sequence |
