AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0316240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0316240 MtrunA17_Chr2g0288061 86.301 73 10 0 8 80 11 83 4.13e-39 135
Msa0316240 MtrunA17_Chr2g0288061 93.846 65 3 1 205 269 124 187 1.60e-33 120
Msa0316240 MtrunA17_Chr2g0287401 51.351 74 36 0 1 74 2 75 3.31e-15 71.6
Msa0316240 MtrunA17_Chr2g0286581 59.259 54 22 0 1 54 12 65 1.68e-14 70.5
Msa0316240 MtrunA17_Chr2g0287311 48.649 74 38 0 1 74 12 85 2.69e-14 70.1
Msa0316240 MtrunA17_Chr2g0287371 48.305 118 40 1 1 118 5 101 4.00e-14 70.1
Msa0316240 MtrunA17_Chr2g0287321 52.055 73 35 0 1 73 11 83 6.69e-14 68.6
Msa0316240 MtrunA17_Chr1g0156951 48.438 64 29 1 1 64 1 60 1.13e-11 63.2
Msa0316240 MtrunA17_Chr4g0019961 47.143 70 37 0 1 70 1 70 6.21e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 35 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTCGAAAAGAAGAGGATT+TGG 0.329420 2_4:-13120713 Msa0316240:CDS
AACGGACCTGCAGAATGTTT+TGG 0.341546 2_4:+13120155 None:intergenic
GGAAGACTTTAATCGTTTAA+CGG 0.443117 2_4:+13120741 None:intergenic
CATTCATGACTTGAGATAAA+CGG 0.459596 2_4:+13120137 None:intergenic
GGCGCTGATGGCGGAAGGCA+CGG 0.460933 2_4:-13120950 None:intergenic
CGCTTGGTTCAACTCTAGAA+AGG 0.462208 2_4:+13120881 None:intergenic
AGAAGAAGTAGATGATGAAG+AGG 0.475837 2_4:-13120683 Msa0316240:CDS
CGAGTTGCTTCGAAAAGAAG+AGG 0.487698 2_4:-13120719 Msa0316240:CDS
CAAGCGTTTATCTGCAATTG+AGG 0.488368 2_4:-13120864 Msa0316240:CDS
ATTTCGATAATCAGATCCTC+CGG 0.496089 2_4:+13120904 None:intergenic
CTCAATTGCAGATAAACGCT+TGG 0.514934 2_4:+13120865 None:intergenic
GTGTCTCCAAAACATTCTGC+AGG 0.545478 2_4:-13120161 Msa0316240:CDS
TATTGAGTTTCTGCGGCAGA+AGG 0.548094 2_4:-13120284 Msa0316240:CDS
GCAGAAGGAGGCAGTTCCGT+TGG 0.553372 2_4:-13120269 Msa0316240:CDS
TGAAGAAGAGGAGAAAGAAT+CGG 0.556114 2_4:-13120320 Msa0316240:CDS
GTCACAGAACGCGATACTCC+CGG 0.563110 2_4:-13120923 Msa0316240:CDS
TGGTGGTGGTTCTGAAGAAG+AGG 0.571049 2_4:-13120332 Msa0316240:CDS
CGAAAAGAAGAGGATTTGGA+TGG 0.572187 2_4:-13120709 Msa0316240:CDS
AATTGAGGTGCGTATGCAAA+TGG 0.587049 2_4:-13120849 Msa0316240:CDS
ACGACGGCGCTGATGGCGGA+AGG 0.604197 2_4:-13120955 None:intergenic
TGAGTTTCTGCGGCAGAAGG+AGG 0.614751 2_4:-13120281 Msa0316240:CDS
TTTCGATAATCAGATCCTCC+GGG 0.622988 2_4:+13120905 None:intergenic
TTTGATGATGATGAAGACAA+TGG 0.624005 2_4:-13120535 Msa0316240:CDS
CAGAGATGAAATGCAAATAG+CGG 0.644769 2_4:-13120197 Msa0316240:CDS
GTTGATGAAGACAATGGTGG+TGG 0.645971 2_4:-13120346 Msa0316240:CDS
AAGTAGTTATTGAGTTTCTG+CGG 0.650429 2_4:-13120291 Msa0316240:CDS
AGATGAAATGCAAATAGCGG+AGG 0.653702 2_4:-13120194 Msa0316240:CDS
GATGATGAAGACAATGGTGG+TGG 0.658810 2_4:-13120529 Msa0316240:CDS
GAGAATGTTGATGAAGACAA+TGG 0.662623 2_4:-13120352 Msa0316240:CDS
TGAGGTGCGTATGCAAATGG+TGG 0.665483 2_4:-13120846 Msa0316240:CDS
GATGATGATGAAGACAATGG+TGG 0.671380 2_4:-13120532 Msa0316240:CDS
AAGAGGAGAAAGAATCGGCG+TGG 0.679826 2_4:-13120315 Msa0316240:CDS
AATGTTGATGAAGACAATGG+TGG 0.715735 2_4:-13120349 Msa0316240:CDS
GCTGATGGCGGAAGGCACGG+CGG 0.728631 2_4:-13120947 None:intergenic
ACAGAACGCGATACTCCCGG+AGG 0.809853 2_4:-13120920 Msa0316240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTGAAGATTTTG+AGG - chr2_4:13120829-13120848 Msa0316240:CDS 15.0%
!!! TAATTTTTTGAAGATTTTGA+GGG - chr2_4:13120830-13120849 Msa0316240:CDS 15.0%
!!! ATAATTTTTTGAAGATTTTG+AGG - chr2_4:13120829-13120848 Msa0316240:CDS 15.0%
!!! TAATTTTTTGAAGATTTTGA+GGG - chr2_4:13120830-13120849 Msa0316240:CDS 15.0%
!!! TGTTTTTGATCGTTATTTAT+TGG - chr2_4:13120260-13120279 Msa0316240:CDS 20.0%
!! ATCTTCAAAAAATTATCCAA+CGG + chr2_4:13120825-13120844 None:intergenic 20.0%
!!! AATTTTTTGAAGATTTTGAG+GGG - chr2_4:13120831-13120850 Msa0316240:CDS 20.0%
!!! TGTTTTTGATCGTTATTTAT+TGG - chr2_4:13120260-13120279 Msa0316240:CDS 20.0%
!! ATCTTCAAAAAATTATCCAA+CGG + chr2_4:13120825-13120844 None:intergenic 20.0%
!!! AATTTTTTGAAGATTTTGAG+GGG - chr2_4:13120831-13120850 Msa0316240:CDS 20.0%
GGAAGACTTTAATCGTTTAA+CGG + chr2_4:13120337-13120356 None:intergenic 30.0%
!! TTTGATGATGATGAAGACAA+TGG - chr2_4:13120540-13120559 Msa0316240:CDS 30.0%
AAGTAGTTATTGAGTTTCTG+CGG - chr2_4:13120784-13120803 Msa0316240:CDS 30.0%
CATTCATGACTTGAGATAAA+CGG + chr2_4:13120941-13120960 None:intergenic 30.0%
GGAAGACTTTAATCGTTTAA+CGG + chr2_4:13120337-13120356 None:intergenic 30.0%
!! TTTGATGATGATGAAGACAA+TGG - chr2_4:13120540-13120559 Msa0316240:CDS 30.0%
AAGTAGTTATTGAGTTTCTG+CGG - chr2_4:13120784-13120803 Msa0316240:CDS 30.0%
CATTCATGACTTGAGATAAA+CGG + chr2_4:13120941-13120960 None:intergenic 30.0%
ATTTCGATAATCAGATCCTC+CGG + chr2_4:13120174-13120193 None:intergenic 35.0%
AGAAGAAGTAGATGATGAAG+AGG - chr2_4:13120392-13120411 Msa0316240:CDS 35.0%
! GAGAATGTTGATGAAGACAA+TGG - chr2_4:13120723-13120742 Msa0316240:CDS 35.0%
! AATGTTGATGAAGACAATGG+TGG - chr2_4:13120726-13120745 Msa0316240:CDS 35.0%
TGAAGAAGAGGAGAAAGAAT+CGG - chr2_4:13120755-13120774 Msa0316240:CDS 35.0%
CAGAGATGAAATGCAAATAG+CGG - chr2_4:13120878-13120897 Msa0316240:CDS 35.0%
ATTTCGATAATCAGATCCTC+CGG + chr2_4:13120174-13120193 None:intergenic 35.0%
AGAAGAAGTAGATGATGAAG+AGG - chr2_4:13120392-13120411 Msa0316240:CDS 35.0%
! GAGAATGTTGATGAAGACAA+TGG - chr2_4:13120723-13120742 Msa0316240:CDS 35.0%
! AATGTTGATGAAGACAATGG+TGG - chr2_4:13120726-13120745 Msa0316240:CDS 35.0%
TGAAGAAGAGGAGAAAGAAT+CGG - chr2_4:13120755-13120774 Msa0316240:CDS 35.0%
CAGAGATGAAATGCAAATAG+CGG - chr2_4:13120878-13120897 Msa0316240:CDS 35.0%
TTTCGATAATCAGATCCTCC+GGG + chr2_4:13120173-13120192 None:intergenic 40.0%
CTCAATTGCAGATAAACGCT+TGG + chr2_4:13120213-13120232 None:intergenic 40.0%
CAAGCGTTTATCTGCAATTG+AGG - chr2_4:13120211-13120230 Msa0316240:CDS 40.0%
AATTGAGGTGCGTATGCAAA+TGG - chr2_4:13120226-13120245 Msa0316240:CDS 40.0%
! CTCTTCTTTTCGAAGCAACT+CGG + chr2_4:13120358-13120377 None:intergenic 40.0%
GCTTCGAAAAGAAGAGGATT+TGG - chr2_4:13120362-13120381 Msa0316240:CDS 40.0%
CGAAAAGAAGAGGATTTGGA+TGG - chr2_4:13120366-13120385 Msa0316240:CDS 40.0%
! GATGATGATGAAGACAATGG+TGG - chr2_4:13120543-13120562 Msa0316240:CDS 40.0%
AGATGAAATGCAAATAGCGG+AGG - chr2_4:13120881-13120900 Msa0316240:CDS 40.0%
TTTCGATAATCAGATCCTCC+GGG + chr2_4:13120173-13120192 None:intergenic 40.0%
CTCAATTGCAGATAAACGCT+TGG + chr2_4:13120213-13120232 None:intergenic 40.0%
CAAGCGTTTATCTGCAATTG+AGG - chr2_4:13120211-13120230 Msa0316240:CDS 40.0%
AATTGAGGTGCGTATGCAAA+TGG - chr2_4:13120226-13120245 Msa0316240:CDS 40.0%
! CTCTTCTTTTCGAAGCAACT+CGG + chr2_4:13120358-13120377 None:intergenic 40.0%
GCTTCGAAAAGAAGAGGATT+TGG - chr2_4:13120362-13120381 Msa0316240:CDS 40.0%
CGAAAAGAAGAGGATTTGGA+TGG - chr2_4:13120366-13120385 Msa0316240:CDS 40.0%
! GATGATGATGAAGACAATGG+TGG - chr2_4:13120543-13120562 Msa0316240:CDS 40.0%
AGATGAAATGCAAATAGCGG+AGG - chr2_4:13120881-13120900 Msa0316240:CDS 40.0%
! CGCTTGGTTCAACTCTAGAA+AGG + chr2_4:13120197-13120216 None:intergenic 45.0%
CGAGTTGCTTCGAAAAGAAG+AGG - chr2_4:13120356-13120375 Msa0316240:CDS 45.0%
GATGATGAAGACAATGGTGG+TGG - chr2_4:13120546-13120565 Msa0316240:CDS 45.0%
! GTTGATGAAGACAATGGTGG+TGG - chr2_4:13120729-13120748 Msa0316240:CDS 45.0%
TATTGAGTTTCTGCGGCAGA+AGG - chr2_4:13120791-13120810 Msa0316240:CDS 45.0%
GTGTCTCCAAAACATTCTGC+AGG - chr2_4:13120914-13120933 Msa0316240:CDS 45.0%
! AACGGACCTGCAGAATGTTT+TGG + chr2_4:13120923-13120942 None:intergenic 45.0%
! CGCTTGGTTCAACTCTAGAA+AGG + chr2_4:13120197-13120216 None:intergenic 45.0%
CGAGTTGCTTCGAAAAGAAG+AGG - chr2_4:13120356-13120375 Msa0316240:CDS 45.0%
GATGATGAAGACAATGGTGG+TGG - chr2_4:13120546-13120565 Msa0316240:CDS 45.0%
! GTTGATGAAGACAATGGTGG+TGG - chr2_4:13120729-13120748 Msa0316240:CDS 45.0%
TATTGAGTTTCTGCGGCAGA+AGG - chr2_4:13120791-13120810 Msa0316240:CDS 45.0%
GTGTCTCCAAAACATTCTGC+AGG - chr2_4:13120914-13120933 Msa0316240:CDS 45.0%
! AACGGACCTGCAGAATGTTT+TGG + chr2_4:13120923-13120942 None:intergenic 45.0%
TGAGGTGCGTATGCAAATGG+TGG - chr2_4:13120229-13120248 Msa0316240:CDS 50.0%
!! TGGTGGTGGTTCTGAAGAAG+AGG - chr2_4:13120560-13120579 Msa0316240:CDS 50.0%
AAGAGGAGAAAGAATCGGCG+TGG - chr2_4:13120760-13120779 Msa0316240:CDS 50.0%
TGAGGTGCGTATGCAAATGG+TGG - chr2_4:13120229-13120248 Msa0316240:CDS 50.0%
!! TGGTGGTGGTTCTGAAGAAG+AGG - chr2_4:13120560-13120579 Msa0316240:CDS 50.0%
AAGAGGAGAAAGAATCGGCG+TGG - chr2_4:13120760-13120779 Msa0316240:CDS 50.0%
GTCACAGAACGCGATACTCC+CGG - chr2_4:13120152-13120171 Msa0316240:CDS 55.0%
TGAGTTTCTGCGGCAGAAGG+AGG - chr2_4:13120794-13120813 Msa0316240:CDS 55.0%
GTCACAGAACGCGATACTCC+CGG - chr2_4:13120152-13120171 Msa0316240:CDS 55.0%
TGAGTTTCTGCGGCAGAAGG+AGG - chr2_4:13120794-13120813 Msa0316240:CDS 55.0%
ACAGAACGCGATACTCCCGG+AGG - chr2_4:13120155-13120174 Msa0316240:CDS 60.0%
GCAGAAGGAGGCAGTTCCGT+TGG - chr2_4:13120806-13120825 Msa0316240:CDS 60.0%
ACAGAACGCGATACTCCCGG+AGG - chr2_4:13120155-13120174 Msa0316240:CDS 60.0%
GCAGAAGGAGGCAGTTCCGT+TGG - chr2_4:13120806-13120825 Msa0316240:CDS 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 13120132 13120965 13120132 ID=Msa0316240;Name=Msa0316240
chr2_4 mRNA 13120132 13120965 13120132 ID=Msa0316240-mRNA-1;Parent=Msa0316240;Name=Msa0316240-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|-1|0|1|-1|1|1|0|277
chr2_4 exon 13120132 13120965 13120132 ID=Msa0316240-mRNA-1:exon:4991;Parent=Msa0316240-mRNA-1
chr2_4 CDS 13120132 13120965 13120132 ID=Msa0316240-mRNA-1:cds;Parent=Msa0316240-mRNA-1
Gene Sequence

>Msa0316240

ATGGCGGAAGGCACGGCGGAGTCACAGAACGCGATACTCCCGGAGGATCTGATTATCGAAATCCTTTCTAGAGTTGAACCAAGCGTTTATCTGCAATTGAGGTGCGTATGCAAATGGTGGAAATCACTTGTTTTTGATCGTTATTTATTGGAGAAGCACATTCGTATATCGATAACTGAAATCATGTATCTAGTTTGTGATGCCGTTAAACGATTAAAGTCTTCCGAGTTGCTTCGAAAAGAAGAGGATTTGGATGGTGAAGAAGAAGTAGATGATGAAGAGGATTTTGATGATGAAGAAGAAGAAGATTTTGATGAAGAAGAAGAAGAAGAAGTAGATGATGAAGAAGATTTTGATGAAGAAGAAGATGATGATTATGATGATGATGATGAAGATGACGAAGAAGATTTTGATGATGATGAAGACAATGGTGGTGGTTCTGAAGAAGAGAAAGAAGAAGATTTTGATGATGAAGAAGAAGAAGAAGATGAAGAAGATGATGACGAAGAAGATTTTGATGAAGAAGAAGAAGATGATGATGATGAAGATGACGAAGAAGATTTTGATGAAGAAGAAAACAATGCTGAAGAAGAGAATGTTGATGAAGACAATGGTGGTGGTTCTGAAGAAGAGGAGAAAGAATCGGCGTGGAAAGTAGTTATTGAGTTTCTGCGGCAGAAGGAGGCAGTTCCGTTGGATAATTTTTTGAAGATTTTGAGGGGAATCAAAGAGCAATACGAAAGTATCAGAGATGAAATGCAAATAGCGGAGGATAGAGTGAAGTGTCTCCAAAACATTCTGCAGGTCCGTTTATCTCAAGTCATGAATGAGTGA

Protein sequence

>Msa0316240

MAEGTAESQNAILPEDLIIEILSRVEPSVYLQLRCVCKWWKSLVFDRYLLEKHIRISITEIMYLVCDAVKRLKSSELLRKEEDLDGEEEVDDEEDFDDEEEEDFDEEEEEEVDDEEDFDEEEDDDYDDDDEDDEEDFDDDEDNGGGSEEEKEEDFDDEEEEEDEEDDDEEDFDEEEEDDDDEDDEEDFDEEENNAEEENVDEDNGGGSEEEEKESAWKVVIEFLRQKEAVPLDNFLKILRGIKEQYESIRDEMQIAEDRVKCLQNILQVRLSQVMNE*