AlfalfaGEDB | Msa0760350

M. sativa cultivar zhongmu-4 / Msa0760350

KEGG: -
GO: GO:0000981 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0016592 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0070013 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760350	sp\|O80450\|TGT3B_ARATH	42.199	282	138	6	26	301	26	288	9.32e-46	159

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760350	A0A371F1K1	81.818	308	42	5	4	301	35	338	5.19e-173	491

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TFs/TRs:

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Gene ID	Type	Classification
Msa0760350	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003630	Msa0760350	0.817012	4.112452e-52	-8.615850e-47
Msa0005270	Msa0760350	0.819916	9.034571e-53	-8.615850e-47
Msa0006110	Msa0760350	0.809711	1.653270e-50	-8.615850e-47
Msa0007440	Msa0760350	0.802889	4.528275e-49	-8.615850e-47
Msa0008220	Msa0760350	0.818759	1.657478e-52	-8.615850e-47
Msa0010490	Msa0760350	0.815930	7.181144e-52	-8.615850e-47
Msa0011960	Msa0760350	0.801636	8.201662e-49	-8.615850e-47
Msa0015090	Msa0760350	0.821548	3.808816e-53	-8.615850e-47
Msa0017320	Msa0760350	0.853574	2.183352e-61	-8.615850e-47
Msa0021210	Msa0760350	0.838796	2.324713e-57	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760350	MtrunA17_Chr5g0409071	93.046	302	20	1	1	301	1	302	2.48e-175	486
Msa0760350	MtrunA17_Chr4g0006261	49.804	255	108	6	44	296	40	276	3.79e-55	180
Msa0760350	MtrunA17_Chr7g0242591	46.154	273	130	5	28	299	35	291	6.43e-50	167
Msa0760350	MtrunA17_Chr3g0123021	50.820	61	30	0	46	106	77	137	5.46e-14	71.6
Msa0760350	MtrunA17_Chr2g0290511	31.193	109	66	2	30	129	241	349	4.30e-13	68.9
Msa0760350	MtrunA17_Chr1g0201911	33.684	95	60	1	41	132	368	462	1.77e-12	67.8
Msa0760350	MtrunA17_Chr1g0201941	33.696	92	56	3	46	133	455	545	4.31e-11	63.5
Msa0760350	MtrunA17_Chr6g0463941	34.066	91	55	3	46	132	465	554	7.98e-11	62.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0760350	AT2G38250.1	42.199	282	138	6	26	301	26	288	9.48e-47	159
Msa0760350	AT5G01380.1	39.249	293	151	7	26	301	33	315	1.01e-39	142
Msa0760350	AT1G13450.3	34.043	141	87	3	46	183	87	224	8.04e-16	76.3
Msa0760350	AT1G13450.2	34.043	141	87	3	46	183	87	224	8.04e-16	76.3
Msa0760350	AT1G13450.4	33.333	135	86	2	46	179	87	218	2.57e-15	74.3
Msa0760350	AT1G13450.1	34.043	141	87	3	46	183	87	224	2.75e-15	76.3
Msa0760350	AT3G25990.1	39.785	93	48	2	46	132	55	145	2.06e-14	73.2
Msa0760350	AT1G76890.2	34.746	118	72	3	19	132	371	487	2.23e-13	70.9
Msa0760350	AT1G76880.1	34.579	107	60	4	30	132	398	498	2.99e-11	64.3

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Find 101 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGTACGGCGGCGGTGG+AGG	0.483528	5_2:-16759237	Msa0760350:CDS
AAACCGAAAATGTACGGCGG+CGG	0.675954	5_2:-16759243	Msa0760350:exon
AAAGAGCACATAACATGCAA+CGG	0.643374	5_2:-16757467	Msa0760350:CDS
AAGAGAAAGATACCTTATAG+CGG	0.711707	5_2:+16758926	None:intergenic
AAGAGAGTTCATAGCGATAA+GGG	0.460403	5_2:-16759082	Msa0760350:CDS
AAGTGGTGAGTTCGAAGATG+AGG	0.496550	5_2:-16759008	Msa0760350:CDS
AATACACGTAAAAGGAAAGC+TGG	0.551659	5_2:-16757303	Msa0760350:CDS
AATGACTGAACAGGAATGGA+GGG	0.589645	5_2:-16757076	Msa0760350:CDS
AATGGAGATGCAGTGGAGGG+AGG	0.556920	5_2:-16757181	Msa0760350:CDS
AATTGAAGATGACAGTGAAG+AGG	0.562094	5_2:-16757343	Msa0760350:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAGAAAGATACCTTATAG+CGG	+	chr5_2:16757082-16757101	None:intergenic	30.0%
ATCGTTTGATTCCAATTTAG+TGG	-	chr5_2:16757237-16757256	Msa0760350:CDS	30.0%
TCGTTTGATTCCAATTTAGT+GGG	-	chr5_2:16757238-16757257	Msa0760350:CDS	30.0%
AAACACTTATCCCACTAAAT+TGG	+	chr5_2:16757251-16757270	None:intergenic	30.0%
ATTTGGAAGATGAACTAACA+GGG	-	chr5_2:16757323-16757342	Msa0760350:CDS	30.0%
CTTATTTATCCACAGTTCAA+AGG	+	chr5_2:16757645-16757664	None:intergenic	30.0%
GAACTGTGGATAAATAAGTA+TGG	-	chr5_2:16757647-16757666	Msa0760350:intron	30.0%
ATGAACTCAATGCAACATTA+TGG	+	chr5_2:16757674-16757693	None:intergenic	30.0%
ATATGAAACGTTGTGATGAT+TGG	-	chr5_2:16757696-16757715	Msa0760350:intron	30.0%
AAACCCCAATATGTATGATT+TGG	-	chr5_2:16758277-16758296	Msa0760350:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_2	gene	16756632	16759395	16756632	ID=Msa0760350;Name=Msa0760350
chr5_2	mRNA	16756632	16759395	16756632	ID=Msa0760350-mRNA-1;Parent=Msa0760350;Name=Msa0760350-mRNA-1;_AED=0.01;_eAED=0.01;_QI=139\|1\|1\|1\|1\|1\|2\|343\|301
chr5_2	exon	16756632	16757562	16756632	ID=Msa0760350-mRNA-1:exon:7396;Parent=Msa0760350-mRNA-1
chr5_2	exon	16758939	16759395	16758939	ID=Msa0760350-mRNA-1:exon:7395;Parent=Msa0760350-mRNA-1
chr5_2	five_prime_UTR	16759257	16759395	16759257	ID=Msa0760350-mRNA-1:five_prime_utr;Parent=Msa0760350-mRNA-1
chr5_2	CDS	16758939	16759256	16758939	ID=Msa0760350-mRNA-1:cds;Parent=Msa0760350-mRNA-1
chr5_2	CDS	16756975	16757562	16756975	ID=Msa0760350-mRNA-1:cds;Parent=Msa0760350-mRNA-1
chr5_2	three_prime_UTR	16756632	16756974	16756632	ID=Msa0760350-mRNA-1:three_prime_utr;Parent=Msa0760350-mRNA-1

Gene Sequence

>Msa0760350

ATGTACGGCGGCGGTGGAGGAGACGATGCTCTGGCAGCTCGCCTAGGAATAGTACCACCGTCACTTCTGATTCCGACATCGTCGTTGATGTCTCCTGCGGCGGTGAGAGAGGAGAGGGTACCAGCTCAGCCGCAGTGGAGTCAGCAGGAGACAAGAGAGTTCATAGCGATAAGGGCGGAGCTGGAGAGGGATTTTACAGCTTCGAAGAGGAACAAGACGCTTTGGGAAGTGGTGAGTTCGAAGATGAGGGAGAGAGGGTATAGGAGGAGTCCTGAACAGTGTAAATGCAAGTGGAAGAATCTTGTTAACCGCTATAAGGGAAAAGAGACATCTGATCCAGAACATGGCAAGCAATGCCCATTTTTTGAAGAATTGCATGCAGTTTTTACCGAAAGAGCACATAACATGCAACGGTTACTTCTTGAATCCGAAACTCGTTCAGCTCAAACAAAGAAAGGAGTGAAGAAAATAAGAAGTGGAGACCGATCCTCTGACGAATTCTCAGAAGATGATGAAATTGAAGATGACAGTGAAGAGGAGAGACCTGCGAGAAGCAATACACGTAAAAGGAAAGCTGGCAAAATTGCAACGGAGAAGTCTTCAAGGGTAAACAATGCAAACAATAGTACTAGTAATATTCAAGAAATGCTCAAGGAATTTTTCCAGTACCAACTAAAAATGGAGATGCAGTGGAGGGAGGTGATGGATAGGCGGGCACATGAAAGACTGTTGTTTGAACAGGAATGGCGGCAGTCAATGGAAAAGCTTGAGAGGGAGAGATTAATGACTGAACAGGAATGGAGGGAAAGAGAAGAGCATAGGAGACTGAGGGAGGAGAATAGGGCAGAGAGAAGGGACGCTTTGTTGACGACCCTTTTAAACAAACTCATTCATGAATCTAACTAA

Protein sequence

>Msa0760350

MYGGGGGDDALAARLGIVPPSLLIPTSSLMSPAAVREERVPAQPQWSQQETREFIAIRAELERDFTASKRNKTLWEVVSSKMRERGYRRSPEQCKCKWKNLVNRYKGKETSDPEHGKQCPFFEELHAVFTERAHNMQRLLLESETRSAQTKKGVKKIRSGDRSSDEFSEDDEIEDDSEEERPARSNTRKRKAGKIATEKSSRVNNANNSTSNIQEMLKEFFQYQLKMEMQWREVMDRRAHERLLFEQEWRQSMEKLERERLMTEQEWREREEHRRLREENRAERRDALLTTLLNKLIHESN*