AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0735550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735550 MtrunA17_Chr5g0432781 96.358 604 22 0 1 604 1 604 0.0 1187
Msa0735550 MtrunA17_Chr3g0107891 51.195 586 197 11 44 604 15 536 2.93e-173 502
Msa0735550 MtrunA17_Chr6g0483761 31.698 530 302 13 112 604 2 508 3.70e-52 187
Msa0735550 MtrunA17_Chr1g0204981 59.690 129 40 2 16 134 8 134 2.14e-42 164
Msa0735550 MtrunA17_Chr1g0185241 75.862 87 21 0 16 102 10 96 2.25e-40 158
Msa0735550 MtrunA17_Chr5g0394641 75.862 87 21 0 16 102 8 94 1.92e-39 155
Msa0735550 MtrunA17_Chr7g0260161 28.618 615 298 22 16 513 8 598 5.07e-39 154
Msa0735550 MtrunA17_Chr5g0412211 55.556 99 40 2 20 118 9 103 5.44e-31 129
Msa0735550 MtrunA17_Chr8g0367521 44.715 123 58 3 20 132 9 131 1.15e-28 122
Msa0735550 MtrunA17_Chr3g0128621 28.338 367 234 7 237 593 195 542 3.89e-28 119
Msa0735550 MtrunA17_Chr5g0427501 50.505 99 45 1 20 118 9 103 1.18e-25 112
Msa0735550 MtrunA17_Chr7g0268631 64.557 79 25 2 22 97 53 131 6.15e-25 106
Msa0735550 MtrunA17_Chr1g0196081 54.839 93 41 1 8 100 4 95 4.21e-24 106
Msa0735550 MtrunA17_Chr8g0350861 27.575 301 167 5 313 598 12 276 1.05e-18 87.0
Msa0735550 MtrunA17_Chr3g0132861 53.333 75 33 1 30 102 14 88 4.72e-18 88.6
Msa0735550 MtrunA17_Chr8g0350851 30.516 213 139 3 392 596 14 225 6.79e-16 77.4
Msa0735550 MtrunA17_Chr3g0128591 26.349 315 202 6 237 522 69 382 5.79e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0735550 AT5G58320.5 45.364 604 255 12 6 596 6 547 1.29e-134 405
Msa0735550 AT5G58320.2 45.364 604 255 12 6 596 6 547 1.29e-134 405
Msa0735550 AT2G30500.2 43.954 612 240 16 1 604 1 517 1.80e-125 380
Msa0735550 AT2G30500.1 43.954 612 240 16 1 604 1 517 1.80e-125 380
Msa0735550 AT2G30500.3 43.842 609 239 16 4 604 3 516 1.54e-123 375
Msa0735550 AT5G58320.1 50.221 452 200 10 6 448 6 441 4.71e-120 366
Msa0735550 AT5G58320.4 50.221 452 200 10 6 448 6 441 4.71e-120 366
Msa0735550 AT5G58320.3 48.565 418 182 9 44 448 1 398 1.78e-101 315
Msa0735550 AT1G03080.3 74.157 89 23 0 19 107 10 98 1.83e-39 155
Msa0735550 AT1G03080.1 74.157 89 23 0 19 107 10 98 1.83e-39 155
Msa0735550 AT1G03080.2 74.157 89 23 0 19 107 10 98 1.83e-39 155
Msa0735550 AT3G22790.1 75.581 86 20 1 16 101 8 92 2.58e-37 149
Msa0735550 AT3G22790.3 75.581 86 20 1 16 101 8 92 2.58e-37 149
Msa0735550 AT3G22790.2 75.581 86 20 1 16 101 8 92 2.58e-37 149
Msa0735550 AT4G02710.1 68.966 87 27 0 17 103 9 95 9.87e-37 147
Msa0735550 AT4G14760.4 69.318 88 26 1 16 103 8 94 7.41e-35 142
Msa0735550 AT4G14760.2 69.318 88 26 1 16 103 8 94 7.41e-35 142
Msa0735550 AT4G14760.3 69.318 88 26 1 16 103 8 94 7.41e-35 142
Msa0735550 AT4G14760.1 69.318 88 26 1 16 103 8 94 7.41e-35 142
Msa0735550 AT2G22560.1 62.651 83 31 0 20 102 9 91 1.81e-30 127
Msa0735550 AT5G10500.1 43.939 132 71 1 12 140 1 132 6.07e-29 123
Msa0735550 AT1G09720.1 61.446 83 32 0 20 102 9 91 3.48e-28 120
Msa0735550 AT1G58215.1 60.241 83 33 0 20 102 9 91 4.05e-28 120
Msa0735550 AT1G03470.2 62.338 77 28 1 20 95 5 81 7.30e-25 104
Msa0735550 AT1G03470.3 62.338 77 28 1 20 95 5 81 7.30e-25 104
Msa0735550 AT1G03470.1 62.338 77 28 1 20 95 5 81 7.30e-25 104
Msa0735550 AT1G03470.4 62.338 77 28 1 20 95 5 81 7.30e-25 104
Msa0735550 AT2G47920.2 56.098 82 36 0 16 97 4 85 2.82e-24 102
Msa0735550 AT2G47920.1 56.098 82 36 0 16 97 4 85 2.82e-24 102
Msa0735550 AT5G05180.1 30.576 278 150 7 353 595 152 421 1.61e-18 89.0
Msa0735550 AT5G05180.2 30.325 277 128 7 353 595 152 397 2.37e-18 88.2
Msa0735550 AT5G05180.3 30.576 278 150 7 353 595 122 391 3.24e-18 87.8
Msa0735550 AT3G10880.2 44.898 98 53 1 488 584 184 281 5.02e-13 70.5
Msa0735550 AT3G10880.4 44.898 98 53 1 488 584 184 281 5.02e-13 70.5
Msa0735550 AT3G10880.3 44.898 98 53 1 488 584 222 319 5.59e-13 70.9
Msa0735550 AT3G10880.1 44.898 98 53 1 488 584 222 319 5.59e-13 70.9
Msa0735550 AT4G03153.1 41.026 78 41 2 23 95 7 84 6.99e-12 65.9

Find 185 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGGTTTGATTTCTTTCT+TGG 0.129859 5_1:-50938943 Msa0735550:CDS
CAAAATACCAAGATTGAATT+TGG 0.154326 5_1:-50937290 Msa0735550:intron
CATTATCTTATTGTAAAATT+AGG 0.158172 5_1:-50937135 Msa0735550:three_prime_UTR
GCATAAGAGGGTTCCAATTT+TGG 0.168910 5_1:-50937582 Msa0735550:CDS
CTCTGGAAAATGGCTGCTTC+TGG 0.173281 5_1:-50939820 Msa0735550:exon
TCCAAACACACCTTTCTTTC+AGG 0.174928 5_1:-50940764 Msa0735550:intron
ATCCATATTTCTTGAATCTA+AGG 0.206718 5_1:+50938540 None:intergenic
ATGTTCGTATGCATTAATTT+TGG 0.249886 5_1:+50938645 None:intergenic
AATTATTAGGCAGCCAAAAT+TGG 0.250987 5_1:+50937569 None:intergenic
ATCCTTAGATTCAAGAAATA+TGG 0.259814 5_1:-50938542 Msa0735550:CDS
AAAGAGCCACTTAGAATTCT+TGG 0.264250 5_1:+50939616 None:intergenic
TCAACCAAAGCAACCAATTC+TGG 0.267721 5_1:+50939147 None:intergenic
TCAGCTTGACTCGGCTAAAA+AGG 0.286993 5_1:-50938323 Msa0735550:CDS
TTTGATTTCTTTCTTGGAAC+TGG 0.294515 5_1:-50938937 Msa0735550:CDS
TTAGATTTATACTAATTATT+AGG 0.298988 5_1:+50937556 None:intergenic
TCAACATCCGTTTCACATTC+CGG 0.333589 5_1:+50939232 None:intergenic
GAGGCCAGAATTGGTTGCTT+TGG 0.335656 5_1:-50939151 Msa0735550:CDS
AATCAAGGAAATCACATTCT+TGG 0.339438 5_1:-50939661 Msa0735550:CDS
TCAAAGTTAGAAGCTTGATA+TGG 0.350146 5_1:-50937184 Msa0735550:three_prime_UTR
GAGGTAGGTGGTTGCTGCTC+TGG 0.365877 5_1:-50939837 Msa0735550:five_prime_UTR
AAATCAAGCAACCGGGCTGC+TGG 0.367538 5_1:-50938961 Msa0735550:CDS
GACTTCAAATTAGCTTTCTC+GGG 0.368269 5_1:+50938145 None:intergenic
AGAAGCTTGATATGGATATA+AGG 0.370983 5_1:-50937176 Msa0735550:three_prime_UTR
AGACTTCAAATTAGCTTTCT+CGG 0.376529 5_1:+50938144 None:intergenic
TCTGGAAAATGGCTGCTTCT+GGG 0.385154 5_1:-50939819 Msa0735550:exon
AAGAGAAGCGCGAGGCGATC+AGG 0.385466 5_1:-50937670 Msa0735550:CDS
ACTACAAAGACCTTCCTTCC+TGG 0.386183 5_1:-50940970 Msa0735550:five_prime_UTR
CATCAGTGGTCGTTCGATCT+TGG 0.388153 5_1:-50937351 Msa0735550:three_prime_UTR
GAGGTTAAAGACTAGTTTAT+CGG 0.389465 5_1:-50938230 Msa0735550:CDS
ATGGATCTAAGAACAGGAAC+AGG 0.397778 5_1:+50940816 None:intergenic
CAGTGAAGCAACTTGCGTTT+CGG 0.398464 5_1:+50938393 None:intergenic
GTGGTTGCTGCTCTGGAAAA+TGG 0.406053 5_1:-50939830 Msa0735550:exon
GATTTACGATGGGTCACGTT+TGG 0.409430 5_1:+50938979 None:intergenic
AATCACTAAAGAAAATCTTA+AGG 0.409707 5_1:-50938419 Msa0735550:CDS
AAGAGCCACTTAGAATTCTT+GGG 0.412841 5_1:+50939617 None:intergenic
TCGCGGAATGGTACTGATCC+AGG 0.414382 5_1:-50938805 Msa0735550:CDS
GGCGAAGAAACTCGAGGAAC+AGG 0.419693 5_1:-50937729 Msa0735550:CDS
TTTATTCTTCTGTTCATCCC+TGG 0.425227 5_1:+50938787 None:intergenic
CCCATCGTAAATCAAGCAAC+CGG 0.428996 5_1:-50938969 Msa0735550:CDS
TTACAAGAAGAGGCCAGAAT+TGG 0.431957 5_1:-50939160 Msa0735550:CDS
ACCTGAAAGAAAGGTGTGTT+TGG 0.435570 5_1:+50940763 None:intergenic
GGAGAGATTTGAGGAGAGAG+AGG 0.436086 5_1:+50940838 None:intergenic
CCATGTGTAATTCGTTCAAA+TGG 0.438638 5_1:+50937816 None:intergenic
AGGTTTCAGTTTCATCTAGT+GGG 0.439800 5_1:-50938699 Msa0735550:CDS
GTCTGAAATGTCAAAGTTGT+TGG 0.441122 5_1:-50938125 Msa0735550:CDS
GGATACTTGTATTATTATGT+AGG 0.441250 5_1:+50940920 None:intergenic
GAGGAGAGAGAGGTTTGGCT+TGG 0.441700 5_1:+50940848 None:intergenic
GTGGAATTTAAAAGAGGTTG+TGG 0.444884 5_1:-50937213 Msa0735550:three_prime_UTR
AAGCATCGATGGTGTGGATA+AGG 0.448894 5_1:-50938491 Msa0735550:CDS
GAGGTTTCAGTTTCATCTAG+TGG 0.450219 5_1:-50938700 Msa0735550:CDS
AACTCAAGAGACGGTAGGAT+TGG 0.454679 5_1:-50937851 Msa0735550:CDS
GATTTCTTTCTTGGAACTGG+TGG 0.455413 5_1:-50938934 Msa0735550:CDS
TGGCTCTTTGAAAATCTTGA+AGG 0.456114 5_1:-50939602 Msa0735550:intron
AAATGAAGAGGTTGGAATCA+AGG 0.459735 5_1:-50939676 Msa0735550:CDS
AAGCATAAGAAAGACATTAC+AGG 0.464420 5_1:+50937491 None:intergenic
CCCGGTTGCTTGATTTACGA+TGG 0.468876 5_1:+50938968 None:intergenic
GATTTGAGGAGAGAGAGGTT+TGG 0.473241 5_1:+50940843 None:intergenic
CTACCGTGGTTACCGATCAT+TGG 0.475144 5_1:-50939118 Msa0735550:CDS
TCATAGATTCCCCGCATTCG+CGG 0.475457 5_1:+50937241 None:intergenic
CTTGTATTATTATGTAGGGT+GGG 0.478489 5_1:+50940925 None:intergenic
TATGGATGTTGGTGTTGAGT+TGG 0.479532 5_1:-50938524 Msa0735550:CDS
ATTTCTTTCTTGGAACTGGT+GGG 0.483275 5_1:-50938933 Msa0735550:CDS
TTCCCCGCATTCGCGGAGTC+GGG 0.484345 5_1:+50937248 None:intergenic
AGATCCTGCATTCACGCATT+CGG 0.485448 5_1:-50937376 Msa0735550:three_prime_UTR
TTTGGAGAGGGCAAAGTTGA+GGG 0.486062 5_1:+50938997 None:intergenic
AGTTTAGTTACCTGAAAGAA+AGG 0.487239 5_1:+50940754 None:intergenic
ATCTGAGGATTTATTTACCT+CGG 0.488846 5_1:+50938366 None:intergenic
GGAAATCACATTCTTGGTGG+TGG 0.489138 5_1:-50939655 Msa0735550:CDS
CGGACCGGGATCATGAGATC+AGG 0.489204 5_1:-50938210 Msa0735550:CDS
GATAATCTTAATGTAGAAGT+AGG 0.496198 5_1:-50937893 Msa0735550:CDS
ACTTGTATTATTATGTAGGG+TGG 0.497089 5_1:+50940924 None:intergenic
TAAGCAATGGATCTAAGAAC+AGG 0.498793 5_1:+50940810 None:intergenic
GGTTTGGCTTGGTTTGTATG+TGG 0.501642 5_1:+50940859 None:intergenic
ACAGAGAGAGAACAGCATAA+AGG 0.502772 5_1:-50937954 Msa0735550:CDS
TGTCCCGACTCCGCGAATGC+GGG 0.505984 5_1:-50937251 Msa0735550:three_prime_UTR
CAGGAACAGGGAGAGATTTG+AGG 0.507227 5_1:+50940829 None:intergenic
AGTCGGGACAGTCGCATTCA+CGG 0.508331 5_1:+50937264 None:intergenic
CTTTAACCTCAGAGATGGAC+CGG 0.508828 5_1:-50939251 Msa0735550:intron
AGTGAGGCTGTGTCACCACT+TGG 0.510418 5_1:+50938457 None:intergenic
CAGAAATGTATTACAAGAAG+AGG 0.510966 5_1:-50939170 Msa0735550:CDS
ATTGTCAACATCAGGGTTTG+AGG 0.511289 5_1:-50939859 Msa0735550:intron
AATTTCTGCCTTTAGCAACT+CGG 0.511577 5_1:+50937979 None:intergenic
TGAAAGAGCTGATCAGAGAG+TGG 0.513671 5_1:-50938087 Msa0735550:CDS
AGGACTGCATATTGATGTAA+AGG 0.514347 5_1:-50937115 Msa0735550:three_prime_UTR
CCATTTGAACGAATTACACA+TGG 0.514635 5_1:-50937816 Msa0735550:CDS
GAACGTTACGATCATGTGAC+TGG 0.515279 5_1:-50939093 Msa0735550:CDS
TGATCTCCAATCCCAAGGCT+CGG 0.516253 5_1:-50939046 Msa0735550:CDS
TAAAGACTAGTTTATCGGAC+CGG 0.517594 5_1:-50938225 Msa0735550:CDS
ATTCCCCGCATTCGCGGAGT+CGG 0.517955 5_1:+50937247 None:intergenic
GATCTCCAATCCCAAGGCTC+GGG 0.520448 5_1:-50939045 Msa0735550:CDS
AGATAGAATTCAGCAGCTGC+AGG 0.520939 5_1:-50938347 Msa0735550:CDS
ACTAGTGTGGAATTTAAAAG+AGG 0.521079 5_1:-50937219 Msa0735550:three_prime_UTR
CGATGGGTCACGTTTGGAGA+GGG 0.521590 5_1:+50938985 None:intergenic
CTGGAAAATGGCTGCTTCTG+GGG 0.522334 5_1:-50939818 Msa0735550:intron
CGTAACGTTCGGCCAATGAT+CGG 0.523697 5_1:+50939106 None:intergenic
CCGGTTGCTTGATTTACGAT+GGG 0.528312 5_1:+50938969 None:intergenic
TAGCAAGCAAATGAAGAGGT+TGG 0.529450 5_1:-50939684 Msa0735550:CDS
TATTCAGCTTTCTCGCGGAA+TGG 0.530197 5_1:-50938817 Msa0735550:CDS
GATACTTGTATTATTATGTA+GGG 0.531861 5_1:+50940921 None:intergenic
GAACAGAAGAATAAACGAGT+TGG 0.532601 5_1:-50938779 Msa0735550:CDS
AGTGGTGACACAGCCTCACT+CGG 0.538297 5_1:-50938454 Msa0735550:CDS
GATTCAAGAAATATGGATGT+TGG 0.539714 5_1:-50938535 Msa0735550:CDS
ATATAAGGCAAAGCTTGAAA+AGG 0.541553 5_1:-50937161 Msa0735550:three_prime_UTR
ATGCAGGATCTACTCACAGC+AGG 0.541619 5_1:+50937388 None:intergenic
AATCAAGCAACCGGGCTGCT+GGG 0.542696 5_1:-50938960 Msa0735550:CDS
AGTAGGTTCACTCAAGGAAG+AGG 0.544799 5_1:-50937876 Msa0735550:CDS
CTCTACTATGACGGGTTCCG+AGG 0.544882 5_1:-50938869 Msa0735550:CDS
GTTTGGAGAGGGCAAAGTTG+AGG 0.545858 5_1:+50938996 None:intergenic
AAGAAAGTGGCCAGGAAGGA+AGG 0.548239 5_1:+50940960 None:intergenic
AAATGGAACCGAGTTGCTAA+AGG 0.551194 5_1:-50937987 Msa0735550:CDS
CTGTCCCGACTCCGCGAATG+CGG 0.551210 5_1:-50937252 Msa0735550:three_prime_UTR
GGAAAAGAAAGTGGCCAGGA+AGG 0.553367 5_1:+50940956 None:intergenic
GGTGTTGAGTTGGAAGTTGA+AGG 0.555150 5_1:-50938514 Msa0735550:CDS
CAAGGAAATCACATTCTTGG+TGG 0.558813 5_1:-50939658 Msa0735550:CDS
TGTACAACTGATTTCTGCAA+TGG 0.559967 5_1:-50937789 Msa0735550:CDS
TCGAAGCTCCTCCCCGAGTG+AGG 0.560954 5_1:+50938441 None:intergenic
AGATGGACCGGAATGTGAAA+CGG 0.562058 5_1:-50939239 Msa0735550:CDS
AGTCACATGATCGTAACGTT+CGG 0.563685 5_1:+50939095 None:intergenic
GATGAGTCCTCTACTATGAC+GGG 0.566905 5_1:-50938877 Msa0735550:CDS
AAAGACTAGTTTATCGGACC+GGG 0.567985 5_1:-50938224 Msa0735550:CDS
AGAAATCAAACCCAGCAGCC+CGG 0.570169 5_1:+50938950 None:intergenic
TTGTATTATTATGTAGGGTG+GGG 0.570369 5_1:+50940926 None:intergenic
GTCCCGACTCCGCGAATGCG+GGG 0.573756 5_1:-50937250 Msa0735550:three_prime_UTR
AGTCCCTGATCTCATGATCC+CGG 0.574038 5_1:+50938206 None:intergenic
CGCGGAATGGTACTGATCCA+GGG 0.575403 5_1:-50938804 Msa0735550:CDS
GAAATCACATTCTTGGTGGT+GGG 0.575639 5_1:-50939654 Msa0735550:CDS
GTGGTGACACAGCCTCACTC+GGG 0.576029 5_1:-50938453 Msa0735550:CDS
GAAAGAGCTGATCAGAGAGT+GGG 0.576449 5_1:-50938086 Msa0735550:CDS
GAAGTTGAAGGAAGCATCGA+TGG 0.578625 5_1:-50938502 Msa0735550:CDS
GGATGAATCTGATGATTCAT+CGG 0.580721 5_1:-50938848 Msa0735550:CDS
TCAATCCGAAAAGATAGAGA+TGG 0.581551 5_1:-50938023 Msa0735550:CDS
TATGTAGGGTGGGGACACAT+AGG 0.585645 5_1:+50940935 None:intergenic
TGTTGTTATAATCTGAGTCA+AGG 0.587678 5_1:-50937469 Msa0735550:three_prime_UTR
ATTCTAGCAAGCAAATGAAG+AGG 0.587841 5_1:-50939688 Msa0735550:CDS
GAAGCTGAGAGGTTAAGCAA+TGG 0.588507 5_1:+50940797 None:intergenic
TGAAGGAAGCATCGATGGTG+TGG 0.589058 5_1:-50938497 Msa0735550:CDS
AGATGAGTCCTCTACTATGA+CGG 0.591582 5_1:-50938878 Msa0735550:CDS
TGCTCCGAATGCGTGAATGC+AGG 0.595850 5_1:+50937372 None:intergenic
CTCGGAACCCGTCATAGTAG+AGG 0.596535 5_1:+50938870 None:intergenic
CATAGGAAAAGAAAGTGGCC+AGG 0.601833 5_1:+50940952 None:intergenic
ACTGCATATTGATGTAAAGG+AGG 0.602626 5_1:-50937112 Msa0735550:three_prime_UTR
AACTCGAGGAACAGGTGGAG+AGG 0.604467 5_1:-50937721 Msa0735550:CDS
CGGGGAATCTATGACTAGTG+TGG 0.606271 5_1:-50937232 Msa0735550:three_prime_UTR
TGCAGGATCTACTCACAGCA+GGG 0.606778 5_1:+50937389 None:intergenic
ACAATTATTCAGCTTTCTCG+CGG 0.609429 5_1:-50938822 Msa0735550:CDS
ACGATGGGTCACGTTTGGAG+AGG 0.616774 5_1:+50938984 None:intergenic
GGTTTCAGTTTCATCTAGTG+GGG 0.617920 5_1:-50938698 Msa0735550:CDS
CCATCGTAAATCAAGCAACC+GGG 0.618032 5_1:-50938968 Msa0735550:CDS
CAATCTGATCTCCAATCCCA+AGG 0.619499 5_1:-50939051 Msa0735550:CDS
AGCAGCTGAAGAGAAGCGCG+AGG 0.621012 5_1:-50937678 Msa0735550:CDS
CTGCATATTGATGTAAAGGA+GGG 0.621372 5_1:-50937111 Msa0735550:three_prime_UTR
TGTAGAAGTAGGTTCACTCA+AGG 0.622038 5_1:-50937882 Msa0735550:CDS
TGAATCATCAGATTCATCCT+CGG 0.623472 5_1:+50938852 None:intergenic
TCAGGGTTTGAGGAGGTAGG+TGG 0.624311 5_1:-50939849 Msa0735550:intron
GGACCGGGATCATGAGATCA+GGG 0.626205 5_1:-50938209 Msa0735550:CDS
GTCAACATCAGGGTTTGAGG+AGG 0.630741 5_1:-50939856 Msa0735550:intron
GATGTTGAAGCTGATAGAAG+AGG 0.631830 5_1:-50939217 Msa0735550:CDS
TCACGCATTCGGAGCATCAG+TGG 0.631832 5_1:-50937365 Msa0735550:three_prime_UTR
CAGCTGCTGAATTCTATCTG+AGG 0.637838 5_1:+50938351 None:intergenic
TCAGTCCCAAGAATTCTAAG+TGG 0.638403 5_1:-50939622 Msa0735550:CDS
AGGTGAACTCAAGAGACGGT+AGG 0.638743 5_1:-50937856 Msa0735550:CDS
ATGAAGTAGAAGAAGCTGAG+AGG 0.643755 5_1:+50940786 None:intergenic
TTGGTTGAAGAGTTCTACCG+TGG 0.644157 5_1:-50939132 Msa0735550:CDS
AGTAGAAGAGATGAAAACAA+AGG 0.648447 5_1:-50937750 Msa0735550:CDS
TATAATGGGAGACAGAGTAG+TGG 0.654606 5_1:+50937085 None:intergenic
ACATCAGGGTTTGAGGAGGT+AGG 0.654611 5_1:-50939852 Msa0735550:intron
GAAGAGGTGAACTCAAGAGA+CGG 0.656685 5_1:-50937860 Msa0735550:CDS
GAAGAAACTCGAGGAACAGG+TGG 0.656748 5_1:-50937726 Msa0735550:CDS
ATCAGAGAGTGGGAAAGCCG+CGG 0.659031 5_1:-50938076 Msa0735550:CDS
ACAGGAAGGTGAACATGCAG+AGG 0.661050 5_1:-50938719 Msa0735550:CDS
GCTGCAGGATCAGCTTGACT+CGG 0.679769 5_1:-50938332 Msa0735550:CDS
GGAGAGGGCAAAGTTGAGGG+CGG 0.681487 5_1:+50939000 None:intergenic
AAGGAATTGTTCGAGCCAAG+TGG 0.683149 5_1:-50938472 Msa0735550:CDS
TGGTGACACAGCCTCACTCG+GGG 0.683452 5_1:-50938452 Msa0735550:CDS
ACTTCAAATTAGCTTTCTCG+GGG 0.683797 5_1:+50938146 None:intergenic
TGACACAGCCTCACTCGGGG+AGG 0.685440 5_1:-50938449 Msa0735550:CDS
GTTGGAAAATGAACTGCGCG+AGG 0.688241 5_1:-50938761 Msa0735550:CDS
GGACACATAGGAAAAGAAAG+TGG 0.690759 5_1:+50940947 None:intergenic
ATCATGTGACTGGAGAATTG+CGG 0.692757 5_1:-50939083 Msa0735550:CDS
TGGATCTAAGAACAGGAACA+GGG 0.696384 5_1:+50940817 None:intergenic
AACTTCAAGAAAGACTTGCG+AGG 0.705338 5_1:-50938249 Msa0735550:CDS
AACAAAGGCGAAGAAACTCG+AGG 0.715395 5_1:-50937735 Msa0735550:CDS
GCTCTTCAAATGATCGACCG+CGG 0.720469 5_1:+50938059 None:intergenic
CGGCCAATGATCGGTAACCA+CGG 0.730068 5_1:+50939115 None:intergenic
AGTTGCTTCACTGAAAACCG+AGG 0.737756 5_1:-50938383 Msa0735550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATAATAAGTGATT+TGG + chr5_1:50937344-50937363 None:intergenic 10.0%
!! TATGTTTATTATTACTTTAA+TGG - chr5_1:50938616-50938635 Msa0735550:CDS 10.0%
!! TTAGATTTATACTAATTATT+AGG + chr5_1:50940506-50940525 None:intergenic 10.0%
!! ATAGAATGTTAATTATCTTT+AGG - chr5_1:50938764-50938783 Msa0735550:CDS 15.0%
!!! TAAAGATAATTTTGTCAAAA+CGG + chr5_1:50938793-50938812 None:intergenic 15.0%
!! CATTATCTTATTGTAAAATT+AGG - chr5_1:50940924-50940943 Msa0735550:five_prime_UTR 15.0%
!! GATACTTGTATTATTATGTA+GGG + chr5_1:50937141-50937160 None:intergenic 20.0%
!! AAAAATATGAAGATGTTGAA+GGG + chr5_1:50937163-50937182 None:intergenic 20.0%
!! TAAAAATATGAAGATGTTGA+AGG + chr5_1:50937164-50937183 None:intergenic 20.0%
!!! TGTCTTTTTTTCTTTATGAA+AGG + chr5_1:50937552-50937571 None:intergenic 20.0%
!! AATTAAATGTTACATCAATG+TGG + chr5_1:50938106-50938125 None:intergenic 20.0%
!!! AATTAACTTGCACATAATAA+TGG - chr5_1:50938323-50938342 Msa0735550:CDS 20.0%
!!! CTACTTTAAAACTTTTTTGT+TGG - chr5_1:50938738-50938757 Msa0735550:CDS 20.0%
!! AATCACTAAAGAAAATCTTA+AGG - chr5_1:50939640-50939659 Msa0735550:CDS 20.0%
!!! TGATGGTTTTAATTTGAATT+CGG - chr5_1:50940644-50940663 Msa0735550:intron 20.0%
!!! CTGTATTTATTGCATTTTTT+TGG + chr5_1:50940735-50940754 None:intergenic 20.0%
! GGATACTTGTATTATTATGT+AGG + chr5_1:50937142-50937161 None:intergenic 25.0%
!! ATACAAAAACAGCTGAATTT+TGG + chr5_1:50937368-50937387 None:intergenic 25.0%
!! ATTATCTTTGAGAAACAACT+GGG + chr5_1:50937803-50937822 None:intergenic 25.0%
!! TATTATCTTTGAGAAACAAC+TGG + chr5_1:50937804-50937823 None:intergenic 25.0%
!! TTGTCTAAGTCTTTTCATTT+TGG - chr5_1:50938042-50938061 Msa0735550:CDS 25.0%
!! TGTCTAAGTCTTTTCATTTT+GGG - chr5_1:50938043-50938062 Msa0735550:CDS 25.0%
! GACAATAATAAACACAAAAC+AGG + chr5_1:50938186-50938205 None:intergenic 25.0%
! TGTTTATTATTGTCAACATC+AGG - chr5_1:50938192-50938211 Msa0735550:CDS 25.0%
! GTTTATTATTGTCAACATCA+GGG - chr5_1:50938193-50938212 Msa0735550:CDS 25.0%
!!! GTAATACATTTCTGCTTTTT+TGG + chr5_1:50938883-50938902 None:intergenic 25.0%
!! ATGTTCGTATGCATTAATTT+TGG + chr5_1:50939417-50939436 None:intergenic 25.0%
! ATCCTTAGATTCAAGAAATA+TGG - chr5_1:50939517-50939536 Msa0735550:intron 25.0%
! ATCCATATTTCTTGAATCTA+AGG + chr5_1:50939522-50939541 None:intergenic 25.0%
!!! TCTCTAGTTTTAAGTTATCA+AGG + chr5_1:50940144-50940163 None:intergenic 25.0%
! GATAATCTTAATGTAGAAGT+AGG - chr5_1:50940166-50940185 Msa0735550:intron 25.0%
!! ATGCTTAAGCAACATTTTAT+AGG - chr5_1:50940454-50940473 Msa0735550:intron 25.0%
!! TGCTTAAGCAACATTTTATA+GGG - chr5_1:50940455-50940474 Msa0735550:intron 25.0%
!!! AAGTGATAAAGATGTTTTGA+TGG - chr5_1:50940627-50940646 Msa0735550:intron 25.0%
! CAAAATACCAAGATTGAATT+TGG - chr5_1:50940769-50940788 Msa0735550:five_prime_UTR 25.0%
TTGTATTATTATGTAGGGTG+GGG + chr5_1:50937136-50937155 None:intergenic 30.0%
CTTGTATTATTATGTAGGGT+GGG + chr5_1:50937137-50937156 None:intergenic 30.0%
ACTTGTATTATTATGTAGGG+TGG + chr5_1:50937138-50937157 None:intergenic 30.0%
AGTTTAGTTACCTGAAAGAA+AGG + chr5_1:50937308-50937327 None:intergenic 30.0%
AAATGAAATCCCTTGTCATA+TGG - chr5_1:50937451-50937470 Msa0735550:three_prime_UTR 30.0%
AGATAAGAACCATATGACAA+GGG + chr5_1:50937463-50937482 None:intergenic 30.0%
AAGATAAGAACCATATGACA+AGG + chr5_1:50937464-50937483 None:intergenic 30.0%
TTTGTCGAAAAATCCAAAAC+TGG + chr5_1:50937520-50937539 None:intergenic 30.0%
AACTTGCACATAATAATGGT+TGG - chr5_1:50938327-50938346 Msa0735550:CDS 30.0%
AATCAAGGAAATCACATTCT+TGG - chr5_1:50938398-50938417 Msa0735550:CDS 30.0%
TATTACTTTAATGGCCTTCT+TGG - chr5_1:50938625-50938644 Msa0735550:CDS 30.0%
ATTACTTTAATGGCCTTCTT+GGG - chr5_1:50938626-50938645 Msa0735550:CDS 30.0%
AAACTCTTCAACTTGTATGT+CGG - chr5_1:50938670-50938689 Msa0735550:CDS 30.0%
ATTATCTTTAACCTCAGAGA+TGG - chr5_1:50938803-50938822 Msa0735550:CDS 30.0%
CAGAAATGTATTACAAGAAG+AGG - chr5_1:50938889-50938908 Msa0735550:CDS 30.0%
! TTTGATTTCTTTCTTGGAAC+TGG - chr5_1:50939122-50939141 Msa0735550:CDS 30.0%
TCTGATATAAGCCAAAAAGA+CGG - chr5_1:50939155-50939174 Msa0735550:CDS 30.0%
AAAAGAAAAACTTTGGACAC+AGG - chr5_1:50939322-50939341 Msa0735550:intron 30.0%
!! AATTTTGGCATAAACATCGT+CGG + chr5_1:50939402-50939421 None:intergenic 30.0%
GATTCAAGAAATATGGATGT+TGG - chr5_1:50939524-50939543 Msa0735550:intron 30.0%
ATCTGAGGATTTATTTACCT+CGG + chr5_1:50939696-50939715 None:intergenic 30.0%
!! GAGGTTAAAGACTAGTTTAT+CGG - chr5_1:50939829-50939848 Msa0735550:exon 30.0%
!! AGACTTCAAATTAGCTTTCT+CGG + chr5_1:50939918-50939937 None:intergenic 30.0%
ATGGAAGCAGAATTGAAAAA+TGG - chr5_1:50940055-50940074 Msa0735550:intron 30.0%
AGTAGAAGAGATGAAAACAA+AGG - chr5_1:50940309-50940328 Msa0735550:intron 30.0%
! AACATTTTATAGGGCATAAG+AGG - chr5_1:50940464-50940483 Msa0735550:intron 30.0%
! ACATTTTATAGGGCATAAGA+GGG - chr5_1:50940465-50940484 Msa0735550:intron 30.0%
AATTATTAGGCAGCCAAAAT+TGG + chr5_1:50940493-50940512 None:intergenic 30.0%
AAGCATAAGAAAGACATTAC+AGG + chr5_1:50940571-50940590 None:intergenic 30.0%
TGTTGTTATAATCTGAGTCA+AGG - chr5_1:50940590-50940609 Msa0735550:intron 30.0%
ACTAGTGTGGAATTTAAAAG+AGG - chr5_1:50940840-50940859 Msa0735550:five_prime_UTR 30.0%
TCAAAGTTAGAAGCTTGATA+TGG - chr5_1:50940875-50940894 Msa0735550:five_prime_UTR 30.0%
AGAAGCTTGATATGGATATA+AGG - chr5_1:50940883-50940902 Msa0735550:five_prime_UTR 30.0%
ATATAAGGCAAAGCTTGAAA+AGG - chr5_1:50940898-50940917 Msa0735550:five_prime_UTR 30.0%
TAAGCAATGGATCTAAGAAC+AGG + chr5_1:50937252-50937271 None:intergenic 35.0%
! TCCTAAGAAAACACCAGTTT+TGG - chr5_1:50937504-50937523 Msa0735550:three_prime_UTR 35.0%
! TCCAAAACTGGTGTTTTCTT+AGG + chr5_1:50937508-50937527 None:intergenic 35.0%
! TTTCAACGCACTCTCTTTTT+CGG - chr5_1:50937875-50937894 Msa0735550:CDS 35.0%
!! GTTGGCTGTTTTGTTATCTT+AGG - chr5_1:50938345-50938364 Msa0735550:CDS 35.0%
ATTCTAGCAAGCAAATGAAG+AGG - chr5_1:50938371-50938390 Msa0735550:CDS 35.0%
AAATGAAGAGGTTGGAATCA+AGG - chr5_1:50938383-50938402 Msa0735550:CDS 35.0%
AAGAGCCACTTAGAATTCTT+GGG + chr5_1:50938445-50938464 None:intergenic 35.0%
AAAGAGCCACTTAGAATTCT+TGG + chr5_1:50938446-50938465 None:intergenic 35.0%
! TGGCTCTTTGAAAATCTTGA+AGG - chr5_1:50938457-50938476 Msa0735550:CDS 35.0%
TTACTTTAATGGCCTTCTTG+GGG - chr5_1:50938627-50938646 Msa0735550:CDS 35.0%
TCAACTTGTATGTCGGTAAT+TGG - chr5_1:50938677-50938696 Msa0735550:CDS 35.0%
GCAATGGAGTTTCATAATTC+TGG - chr5_1:50938699-50938718 Msa0735550:CDS 35.0%
ATTTCTTTCTTGGAACTGGT+GGG - chr5_1:50939126-50939145 Msa0735550:CDS 35.0%
TGAATCATCAGATTCATCCT+CGG + chr5_1:50939210-50939229 None:intergenic 35.0%
GGATGAATCTGATGATTCAT+CGG - chr5_1:50939211-50939230 Msa0735550:CDS 35.0%
ACAATTATTCAGCTTTCTCG+CGG - chr5_1:50939237-50939256 Msa0735550:CDS 35.0%
TTTATTCTTCTGTTCATCCC+TGG + chr5_1:50939275-50939294 None:intergenic 35.0%
GAACAGAAGAATAAACGAGT+TGG - chr5_1:50939280-50939299 Msa0735550:intron 35.0%
GCGAGGTAAAAGAAAAACTT+TGG - chr5_1:50939315-50939334 Msa0735550:intron 35.0%
AGGTTTCAGTTTCATCTAGT+GGG - chr5_1:50939360-50939379 Msa0735550:intron 35.0%
CTAAAAAGGATGTTGCTACT+TGG - chr5_1:50939750-50939769 Msa0735550:intron 35.0%
! TCTTTTCTCGCTGTTGAATT+TGG + chr5_1:50939780-50939799 None:intergenic 35.0%
!! TAAAGACTAGTTTATCGGAC+CGG - chr5_1:50939834-50939853 Msa0735550:five_prime_UTR 35.0%
!! ACTTCAAATTAGCTTTCTCG+GGG + chr5_1:50939916-50939935 None:intergenic 35.0%
!! GACTTCAAATTAGCTTTCTC+GGG + chr5_1:50939917-50939936 None:intergenic 35.0%
! GTCTGAAATGTCAAAGTTGT+TGG - chr5_1:50939934-50939953 Msa0735550:intron 35.0%
TCAATCCGAAAAGATAGAGA+TGG - chr5_1:50940036-50940055 Msa0735550:intron 35.0%
! TGCTTCCATCTCTATCTTTT+CGG + chr5_1:50940044-50940063 None:intergenic 35.0%
AATTTCTGCCTTTAGCAACT+CGG + chr5_1:50940083-50940102 None:intergenic 35.0%
CCATGTGTAATTCGTTCAAA+TGG + chr5_1:50940246-50940265 None:intergenic 35.0%
CCATTTGAACGAATTACACA+TGG - chr5_1:50940243-50940262 Msa0735550:intron 35.0%
! TGTACAACTGATTTCTGCAA+TGG - chr5_1:50940270-50940289 Msa0735550:intron 35.0%
GTGGAATTTAAAAGAGGTTG+TGG - chr5_1:50940846-50940865 Msa0735550:five_prime_UTR 35.0%
AGGACTGCATATTGATGTAA+AGG - chr5_1:50940944-50940963 Msa0735550:five_prime_UTR 35.0%
ACTGCATATTGATGTAAAGG+AGG - chr5_1:50940947-50940966 Msa0735550:five_prime_UTR 35.0%
CTGCATATTGATGTAAAGGA+GGG - chr5_1:50940948-50940967 Msa0735550:five_prime_UTR 35.0%
GGACACATAGGAAAAGAAAG+TGG + chr5_1:50937115-50937134 None:intergenic 40.0%
TGGATCTAAGAACAGGAACA+GGG + chr5_1:50937245-50937264 None:intergenic 40.0%
ATGGATCTAAGAACAGGAAC+AGG + chr5_1:50937246-50937265 None:intergenic 40.0%
ATGAAGTAGAAGAAGCTGAG+AGG + chr5_1:50937276-50937295 None:intergenic 40.0%
TCCAAACACACCTTTCTTTC+AGG - chr5_1:50937295-50937314 Msa0735550:intron 40.0%
! ACCTGAAAGAAAGGTGTGTT+TGG + chr5_1:50937299-50937318 None:intergenic 40.0%
! AAACGTGGTTGTTGACTAGT+TGG + chr5_1:50937728-50937747 None:intergenic 40.0%
! ACTAGTCAACAACCACGTTT+TGG - chr5_1:50937728-50937747 Msa0735550:CDS 40.0%
! AAGTCTTTTCATTTTGGGCC+TGG - chr5_1:50938048-50938067 Msa0735550:CDS 40.0%
! AGTCTTTTCATTTTGGGCCT+GGG - chr5_1:50938049-50938068 Msa0735550:CDS 40.0%
ATTGTCAACATCAGGGTTTG+AGG - chr5_1:50938200-50938219 Msa0735550:CDS 40.0%
TAGCAAGCAAATGAAGAGGT+TGG - chr5_1:50938375-50938394 Msa0735550:CDS 40.0%
CAAGGAAATCACATTCTTGG+TGG - chr5_1:50938401-50938420 Msa0735550:CDS 40.0%
! GAAATCACATTCTTGGTGGT+GGG - chr5_1:50938405-50938424 Msa0735550:CDS 40.0%
TCAGTCCCAAGAATTCTAAG+TGG - chr5_1:50938437-50938456 Msa0735550:CDS 40.0%
!! TGAGTTTGTTAATGCATGCC+TGG - chr5_1:50938538-50938557 Msa0735550:CDS 40.0%
!! GAGTTTGTTAATGCATGCCT+GGG - chr5_1:50938539-50938558 Msa0735550:CDS 40.0%
CTGTGAGATTGTCAGAATCA+CGG - chr5_1:50938566-50938585 Msa0735550:CDS 40.0%
CAACTATACCAAAACTGCAG+AGG + chr5_1:50938596-50938615 None:intergenic 40.0%
AATCACAAATCTCCCCAAGA+AGG + chr5_1:50938642-50938661 None:intergenic 40.0%
TGTATGTCGGTAATTGGCAA+TGG - chr5_1:50938683-50938702 Msa0735550:CDS 40.0%
TCAACATCCGTTTCACATTC+CGG + chr5_1:50938830-50938849 None:intergenic 40.0%
GATGTTGAAGCTGATAGAAG+AGG - chr5_1:50938842-50938861 Msa0735550:CDS 40.0%
TTACAAGAAGAGGCCAGAAT+TGG - chr5_1:50938899-50938918 Msa0735550:CDS 40.0%
TCAACCAAAGCAACCAATTC+TGG + chr5_1:50938915-50938934 None:intergenic 40.0%
AGTCACATGATCGTAACGTT+CGG + chr5_1:50938967-50938986 None:intergenic 40.0%
ATCATGTGACTGGAGAATTG+CGG - chr5_1:50938976-50938995 Msa0735550:CDS 40.0%
! GCTGGGTTTGATTTCTTTCT+TGG - chr5_1:50939116-50939135 Msa0735550:CDS 40.0%
GATTTCTTTCTTGGAACTGG+TGG - chr5_1:50939125-50939144 Msa0735550:CDS 40.0%
! ACTCATCTTCTCCGTCTTTT+TGG + chr5_1:50939169-50939188 None:intergenic 40.0%
AGATGAGTCCTCTACTATGA+CGG - chr5_1:50939181-50939200 Msa0735550:CDS 40.0%
GAAAAACTTTGGACACAGGA+AGG - chr5_1:50939326-50939345 Msa0735550:intron 40.0%
GAGGTTTCAGTTTCATCTAG+TGG - chr5_1:50939359-50939378 Msa0735550:intron 40.0%
GGTTTCAGTTTCATCTAGTG+GGG - chr5_1:50939361-50939380 Msa0735550:intron 40.0%
!! TATGGATGTTGGTGTTGAGT+TGG - chr5_1:50939535-50939554 Msa0735550:intron 40.0%
AACTTCAAGAAAGACTTGCG+AGG - chr5_1:50939810-50939829 Msa0735550:intron 40.0%
!! AAAGACTAGTTTATCGGACC+GGG - chr5_1:50939835-50939854 Msa0735550:five_prime_UTR 40.0%
AAATGGAACCGAGTTGCTAA+AGG - chr5_1:50940072-50940091 Msa0735550:intron 40.0%
ACAGAGAGAGAACAGCATAA+AGG - chr5_1:50940105-50940124 Msa0735550:intron 40.0%
TGTAGAAGTAGGTTCACTCA+AGG - chr5_1:50940177-50940196 Msa0735550:intron 40.0%
! GCATAAGAGGGTTCCAATTT+TGG - chr5_1:50940477-50940496 Msa0735550:intron 40.0%
ACGGAGTCCAAATTCAATCT+TGG + chr5_1:50940779-50940798 None:intergenic 40.0%
CATAGGAAAAGAAAGTGGCC+AGG + chr5_1:50937110-50937129 None:intergenic 45.0%
!! GGTTTGGCTTGGTTTGTATG+TGG + chr5_1:50937203-50937222 None:intergenic 45.0%
GATTTGAGGAGAGAGAGGTT+TGG + chr5_1:50937219-50937238 None:intergenic 45.0%
GAAGCTGAGAGGTTAAGCAA+TGG + chr5_1:50937265-50937284 None:intergenic 45.0%
!!! TCAACAACCACGTTTTGGAG+CGG - chr5_1:50937733-50937752 Msa0735550:CDS 45.0%
TGATTACGTGATACGACACG+TGG - chr5_1:50937756-50937775 Msa0735550:CDS 45.0%
! TTTTCATTTTGGGCCTGGGT+CGG - chr5_1:50938053-50938072 Msa0735550:CDS 45.0%
AATGTGGACCACACCTACAT+GGG + chr5_1:50938090-50938109 None:intergenic 45.0%
TCTGGAAAATGGCTGCTTCT+GGG - chr5_1:50938240-50938259 Msa0735550:CDS 45.0%
!! TGCTTCTGGGGTAAGTGTTA+TGG - chr5_1:50938253-50938272 Msa0735550:CDS 45.0%
GGAAATCACATTCTTGGTGG+TGG - chr5_1:50938404-50938423 Msa0735550:CDS 45.0%
GACAATCTCACAGTAAACCC+AGG + chr5_1:50938559-50938578 None:intergenic 45.0%
CTTTAACCTCAGAGATGGAC+CGG - chr5_1:50938808-50938827 Msa0735550:CDS 45.0%
AGATGGACCGGAATGTGAAA+CGG - chr5_1:50938820-50938839 Msa0735550:CDS 45.0%
TTGGTTGAAGAGTTCTACCG+TGG - chr5_1:50938927-50938946 Msa0735550:CDS 45.0%
GAACGTTACGATCATGTGAC+TGG - chr5_1:50938966-50938985 Msa0735550:CDS 45.0%
CAATCTGATCTCCAATCCCA+AGG - chr5_1:50939008-50939027 Msa0735550:CDS 45.0%
TTTGGAGAGGGCAAAGTTGA+GGG + chr5_1:50939065-50939084 None:intergenic 45.0%
GATTTACGATGGGTCACGTT+TGG + chr5_1:50939083-50939102 None:intergenic 45.0%
CCGGTTGCTTGATTTACGAT+GGG + chr5_1:50939093-50939112 None:intergenic 45.0%
CCCATCGTAAATCAAGCAAC+CGG - chr5_1:50939090-50939109 Msa0735550:CDS 45.0%
CCATCGTAAATCAAGCAACC+GGG - chr5_1:50939091-50939110 Msa0735550:CDS 45.0%
GATGAGTCCTCTACTATGAC+GGG - chr5_1:50939182-50939201 Msa0735550:CDS 45.0%
TATTCAGCTTTCTCGCGGAA+TGG - chr5_1:50939242-50939261 Msa0735550:CDS 45.0%
!! GGTGTTGAGTTGGAAGTTGA+AGG - chr5_1:50939545-50939564 Msa0735550:intron 45.0%
!! GAAGTTGAAGGAAGCATCGA+TGG - chr5_1:50939557-50939576 Msa0735550:intron 45.0%
!! AAGCATCGATGGTGTGGATA+AGG - chr5_1:50939568-50939587 Msa0735550:intron 45.0%
AAGGAATTGTTCGAGCCAAG+TGG - chr5_1:50939587-50939606 Msa0735550:intron 45.0%
CAGTGAAGCAACTTGCGTTT+CGG + chr5_1:50939669-50939688 None:intergenic 45.0%
AGTTGCTTCACTGAAAACCG+AGG - chr5_1:50939676-50939695 Msa0735550:CDS 45.0%
CAGCTGCTGAATTCTATCTG+AGG + chr5_1:50939711-50939730 None:intergenic 45.0%
AGATAGAATTCAGCAGCTGC+AGG - chr5_1:50939712-50939731 Msa0735550:intron 45.0%
!! TCAGCTTGACTCGGCTAAAA+AGG - chr5_1:50939736-50939755 Msa0735550:intron 45.0%
TGAAAGAGCTGATCAGAGAG+TGG - chr5_1:50939972-50939991 Msa0735550:intron 45.0%
GAAAGAGCTGATCAGAGAGT+GGG - chr5_1:50939973-50939992 Msa0735550:intron 45.0%
AGTAGGTTCACTCAAGGAAG+AGG - chr5_1:50940183-50940202 Msa0735550:intron 45.0%
GAAGAGGTGAACTCAAGAGA+CGG - chr5_1:50940199-50940218 Msa0735550:intron 45.0%
AACTCAAGAGACGGTAGGAT+TGG - chr5_1:50940208-50940227 Msa0735550:intron 45.0%
AACAAAGGCGAAGAAACTCG+AGG - chr5_1:50940324-50940343 Msa0735550:intron 45.0%
AGATCCTGCATTCACGCATT+CGG - chr5_1:50940683-50940702 Msa0735550:intron 45.0%
!! GATAATATAATAATATAAAA+TGG - chr5_1:50937817-50937836 Msa0735550:CDS 5.0%
AAGAAAGTGGCCAGGAAGGA+AGG + chr5_1:50937102-50937121 None:intergenic 50.0%
GGAAAAGAAAGTGGCCAGGA+AGG + chr5_1:50937106-50937125 None:intergenic 50.0%
TATGTAGGGTGGGGACACAT+AGG + chr5_1:50937127-50937146 None:intergenic 50.0%
GGAGAGATTTGAGGAGAGAG+AGG + chr5_1:50937224-50937243 None:intergenic 50.0%
CAGGAACAGGGAGAGATTTG+AGG + chr5_1:50937233-50937252 None:intergenic 50.0%
GTAATCACCGCTCCAAAACG+TGG + chr5_1:50937743-50937762 None:intergenic 50.0%
AATACAGGCCCATGTAGGTG+TGG - chr5_1:50938079-50938098 Msa0735550:CDS 50.0%
CAATGTGGACCACACCTACA+TGG + chr5_1:50938091-50938110 None:intergenic 50.0%
GTCAACATCAGGGTTTGAGG+AGG - chr5_1:50938203-50938222 Msa0735550:CDS 50.0%
ACATCAGGGTTTGAGGAGGT+AGG - chr5_1:50938207-50938226 Msa0735550:CDS 50.0%
!! GTGGTTGCTGCTCTGGAAAA+TGG - chr5_1:50938229-50938248 Msa0735550:CDS 50.0%
! CTCTGGAAAATGGCTGCTTC+TGG - chr5_1:50938239-50938258 Msa0735550:CDS 50.0%
CTGGAAAATGGCTGCTTCTG+GGG - chr5_1:50938241-50938260 Msa0735550:CDS 50.0%
GAGGCCAGAATTGGTTGCTT+TGG - chr5_1:50938908-50938927 Msa0735550:CDS 50.0%
CTACCGTGGTTACCGATCAT+TGG - chr5_1:50938941-50938960 Msa0735550:CDS 50.0%
CGTAACGTTCGGCCAATGAT+CGG + chr5_1:50938956-50938975 None:intergenic 50.0%
TGATCTCCAATCCCAAGGCT+CGG - chr5_1:50939013-50939032 Msa0735550:CDS 50.0%
AAAAACCCGAGCCTTGGGAT+TGG + chr5_1:50939022-50939041 None:intergenic 50.0%
GTCTGAAAAACCCGAGCCTT+GGG + chr5_1:50939027-50939046 None:intergenic 50.0%
TGTCTGAAAAACCCGAGCCT+TGG + chr5_1:50939028-50939047 None:intergenic 50.0%
GTTTGGAGAGGGCAAAGTTG+AGG + chr5_1:50939066-50939085 None:intergenic 50.0%
CCCGGTTGCTTGATTTACGA+TGG + chr5_1:50939094-50939113 None:intergenic 50.0%
AGAAATCAAACCCAGCAGCC+CGG + chr5_1:50939112-50939131 None:intergenic 50.0%
GTTGGAAAATGAACTGCGCG+AGG - chr5_1:50939298-50939317 Msa0735550:intron 50.0%
ACAGGAAGGTGAACATGCAG+AGG - chr5_1:50939340-50939359 Msa0735550:intron 50.0%
!! TGAAGGAAGCATCGATGGTG+TGG - chr5_1:50939562-50939581 Msa0735550:intron 50.0%
AGTCCCTGATCTCATGATCC+CGG + chr5_1:50939856-50939875 None:intergenic 50.0%
GCTCTTCAAATGATCGACCG+CGG + chr5_1:50940003-50940022 None:intergenic 50.0%
AGGTGAACTCAAGAGACGGT+AGG - chr5_1:50940203-50940222 Msa0735550:intron 50.0%
GAAGAAACTCGAGGAACAGG+TGG - chr5_1:50940333-50940352 Msa0735550:intron 50.0%
TGCAGGATCTACTCACAGCA+GGG + chr5_1:50940673-50940692 None:intergenic 50.0%
ATGCAGGATCTACTCACAGC+AGG + chr5_1:50940674-50940693 None:intergenic 50.0%
CATCAGTGGTCGTTCGATCT+TGG - chr5_1:50940708-50940727 Msa0735550:intron 50.0%
TCATAGATTCCCCGCATTCG+CGG + chr5_1:50940821-50940840 None:intergenic 50.0%
CGGGGAATCTATGACTAGTG+TGG - chr5_1:50940827-50940846 Msa0735550:five_prime_UTR 50.0%
GAGGAGAGAGAGGTTTGGCT+TGG + chr5_1:50937214-50937233 None:intergenic 55.0%
GGTGGAATACAGGCCCATGT+AGG - chr5_1:50938074-50938093 Msa0735550:CDS 55.0%
TCAGGGTTTGAGGAGGTAGG+TGG - chr5_1:50938210-50938229 Msa0735550:CDS 55.0%
! ACGGTGAGCCTCTGCAGTTT+TGG - chr5_1:50938585-50938604 Msa0735550:CDS 55.0%
CACATTCCGGTCCATCTCTG+AGG + chr5_1:50938817-50938836 None:intergenic 55.0%
CGGCCAATGATCGGTAACCA+CGG + chr5_1:50938947-50938966 None:intergenic 55.0%
GATCTCCAATCCCAAGGCTC+GGG - chr5_1:50939014-50939033 Msa0735550:CDS 55.0%
!! GGCTCGGGTTTTTCAGACAC+TGG - chr5_1:50939029-50939048 Msa0735550:CDS 55.0%
CGATGGGTCACGTTTGGAGA+GGG + chr5_1:50939077-50939096 None:intergenic 55.0%
ACGATGGGTCACGTTTGGAG+AGG + chr5_1:50939078-50939097 None:intergenic 55.0%
AAATCAAGCAACCGGGCTGC+TGG - chr5_1:50939098-50939117 Msa0735550:CDS 55.0%
AATCAAGCAACCGGGCTGCT+GGG - chr5_1:50939099-50939118 Msa0735550:CDS 55.0%
CTCGGAACCCGTCATAGTAG+AGG + chr5_1:50939192-50939211 None:intergenic 55.0%
!! CTCTACTATGACGGGTTCCG+AGG - chr5_1:50939190-50939209 Msa0735550:CDS 55.0%
!! TCGCGGAATGGTACTGATCC+AGG - chr5_1:50939254-50939273 Msa0735550:intron 55.0%
!! CGCGGAATGGTACTGATCCA+GGG - chr5_1:50939255-50939274 Msa0735550:intron 55.0%
AGTGAGGCTGTGTCACCACT+TGG + chr5_1:50939605-50939624 None:intergenic 55.0%
AGTGGTGACACAGCCTCACT+CGG - chr5_1:50939605-50939624 Msa0735550:CDS 55.0%
GCTGCAGGATCAGCTTGACT+CGG - chr5_1:50939727-50939746 Msa0735550:intron 55.0%
GGACCGGGATCATGAGATCA+GGG - chr5_1:50939850-50939869 Msa0735550:intron 55.0%
ATCAGAGAGTGGGAAAGCCG+CGG - chr5_1:50939983-50940002 Msa0735550:intron 55.0%
GGCGAAGAAACTCGAGGAAC+AGG - chr5_1:50940330-50940349 Msa0735550:intron 55.0%
AACTCGAGGAACAGGTGGAG+AGG - chr5_1:50940338-50940357 Msa0735550:intron 55.0%
TGCTCCGAATGCGTGAATGC+AGG + chr5_1:50940690-50940709 None:intergenic 55.0%
! TCACGCATTCGGAGCATCAG+TGG - chr5_1:50940694-50940713 Msa0735550:intron 55.0%
AGTCGGGACAGTCGCATTCA+CGG + chr5_1:50940798-50940817 None:intergenic 55.0%
! TCATTTTGGGCCTGGGTCGG+TGG - chr5_1:50938056-50938075 Msa0735550:CDS 60.0%
GAGGTAGGTGGTTGCTGCTC+TGG - chr5_1:50938222-50938241 Msa0735550:CDS 60.0%
GGAGAGGGCAAAGTTGAGGG+CGG + chr5_1:50939062-50939081 None:intergenic 60.0%
GTGGTGACACAGCCTCACTC+GGG - chr5_1:50939606-50939625 Msa0735550:CDS 60.0%
TGGTGACACAGCCTCACTCG+GGG - chr5_1:50939607-50939626 Msa0735550:CDS 60.0%
CGGACCGGGATCATGAGATC+AGG - chr5_1:50939849-50939868 Msa0735550:intron 60.0%
! AGCAGCTGAAGAGAAGCGCG+AGG - chr5_1:50940381-50940400 Msa0735550:intron 60.0%
! AAGAGAAGCGCGAGGCGATC+AGG - chr5_1:50940389-50940408 Msa0735550:intron 60.0%
ATTCCCCGCATTCGCGGAGT+CGG + chr5_1:50940815-50940834 None:intergenic 60.0%
GGCCTGGGTCGGTGGAATAC+AGG - chr5_1:50938064-50938083 Msa0735550:CDS 65.0%
GGCCTGTATTCCACCGACCC+AGG + chr5_1:50938069-50938088 None:intergenic 65.0%
TGACACAGCCTCACTCGGGG+AGG - chr5_1:50939610-50939629 Msa0735550:CDS 65.0%
TCGAAGCTCCTCCCCGAGTG+AGG + chr5_1:50939621-50939640 None:intergenic 65.0%
CTGTCCCGACTCCGCGAATG+CGG - chr5_1:50940807-50940826 Msa0735550:five_prime_UTR 65.0%
TGTCCCGACTCCGCGAATGC+GGG - chr5_1:50940808-50940827 Msa0735550:five_prime_UTR 65.0%
TTCCCCGCATTCGCGGAGTC+GGG + chr5_1:50940814-50940833 None:intergenic 65.0%
GTCCCGACTCCGCGAATGCG+GGG - chr5_1:50940809-50940828 Msa0735550:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 50937092 50940989 50937092 ID=Msa0735550;Name=Msa0735550
chr5_1 mRNA 50937092 50940989 50937092 ID=Msa0735550-mRNA-1;Parent=Msa0735550;Name=Msa0735550-mRNA-1;_AED=0.04;_eAED=0.04;_QI=259|1|1|1|0.5|0.2|5|458|604
chr5_1 exon 50937092 50937300 50937092 ID=Msa0735550-mRNA-1:exon:14360;Parent=Msa0735550-mRNA-1
chr5_1 exon 50937325 50939261 50937325 ID=Msa0735550-mRNA-1:exon:14359;Parent=Msa0735550-mRNA-1
chr5_1 exon 50939603 50939714 50939603 ID=Msa0735550-mRNA-1:exon:14358;Parent=Msa0735550-mRNA-1
chr5_1 exon 50939819 50939867 50939819 ID=Msa0735550-mRNA-1:exon:14357;Parent=Msa0735550-mRNA-1
chr5_1 exon 50940765 50940989 50940765 ID=Msa0735550-mRNA-1:exon:14356;Parent=Msa0735550-mRNA-1
chr5_1 five_prime_UTR 50940765 50940989 50940765 ID=Msa0735550-mRNA-1:five_prime_utr;Parent=Msa0735550-mRNA-1
chr5_1 five_prime_UTR 50939834 50939867 50939834 ID=Msa0735550-mRNA-1:five_prime_utr;Parent=Msa0735550-mRNA-1
chr5_1 CDS 50939819 50939833 50939819 ID=Msa0735550-mRNA-1:cds;Parent=Msa0735550-mRNA-1
chr5_1 CDS 50939603 50939714 50939603 ID=Msa0735550-mRNA-1:cds;Parent=Msa0735550-mRNA-1
chr5_1 CDS 50937574 50939261 50937574 ID=Msa0735550-mRNA-1:cds;Parent=Msa0735550-mRNA-1
chr5_1 three_prime_UTR 50937325 50937573 50937325 ID=Msa0735550-mRNA-1:three_prime_utr;Parent=Msa0735550-mRNA-1
chr5_1 three_prime_UTR 50937092 50937300 50937092 ID=Msa0735550-mRNA-1:three_prime_utr;Parent=Msa0735550-mRNA-1
Gene Sequence

>Msa0735550

ATGGCTGCTTCTGGGGTTAATTCTAGCAAGCAAATGAAGAGGTTGGAATCAAGGAAATCACATTCTTGGTGGTGGGATAGTCATATCAGTCCCAAGAATTCTAAGTGGCTCTTTGAAAATCTTGAAGAGATGGACCGGAATGTGAAACGGATGTTGAAGCTGATAGAAGAGGATGCAGATTCATTTGCCAAAAAAGCAGAAATGTATTACAAGAAGAGGCCAGAATTGGTTGCTTTGGTTGAAGAGTTCTACCGTGGTTACCGATCATTGGCCGAACGTTACGATCATGTGACTGGAGAATTGCGGAAGAATGTACAATCTGATCTCCAATCCCAAGGCTCGGGTTTTTCAGACACTGGCTCTGAACCGCCCTCAACTTTGCCCTCTCCAAACGTGACCCATCGTAAATCAAGCAACCGGGCTGCTGGGTTTGATTTCTTTCTTGGAACTGGTGGGAATGCGTCTGATATAAGCCAAAAAGACGGAGAAGATGAGTCCTCTACTATGACGGGTTCCGAGGATGAATCTGATGATTCATCGGTTAACAATTATTCAGCTTTCTCGCGGAATGGTACTGATCCAGGGATGAACAGAAGAATAAACGAGTTGGAAAATGAACTGCGCGAGGTAAAAGAAAAACTTTGGACACAGGAAGGTGAACATGCAGAGGTTTCAGTTTCATCTAGTGGGGCGACATACGAGAATGCCGACGATGTTTATGCCAAAATTAATGCATACGAACATGAATTGATGATTGTGAATGAAAAGTTAAGACTTTCAGAAGAAGAAATCACTAAACTAAAGACTGATCTTGAAAACTACAAATCCTTAGATTCAAGAAATATGGATGTTGGTGTTGAGTTGGAAGTTGAAGGAAGCATCGATGGTGTGGATAAGGAATTGTTCGAGCCAAGTGGTGACACAGCCTCACTCGGGGAGGAGCTTCGAATCACTAAAGAAAATCTTAAGGCTTCCGAAACGCAAGTTGCTTCACTGAAAACCGAGGTAAATAAATCCTCAGATAGAATTCAGCAGCTGCAGGATCAGCTTGACTCGGCTAAAAAGGATGTTGCTACTTGGAAAACCAAATTCAACAGCGAGAAAAGAGAGAGCACGAAACTTCAAGAAAGACTTGCGAGGTTAAAGACTAGTTTATCGGACCGGGATCATGAGATCAGGGACTTGAAAACTGCTGTATCTGATGCTGAGCAGAAAATCTTCCCCGAGAAAGCTAATTTGAAGTCTGAAATGTCAAAGTTGTTGGAAGAACAGACACATTTGAAAGAGCTGATCAGAGAGTGGGAAAGCCGCGGTCGATCATTTGAAGAGCAGATCAGAAACATTCAATCCGAAAAGATAGAGATGGAAGCAGAATTGAAAAATGGAACCGAGTTGCTAAAGGCAGAAATTGAACAGAGAGAGAACAGCATAAAGGATCTCAATGTAAGCCTTGATAACTTAAAACTAGAGAAAGATAATCTTAATGTAGAAGTAGGTTCACTCAAGGAAGAGGTGAACTCAAGAGACGGTAGGATTGGAAGTCTCGATTGCCATTTGAACGAATTACACATGGAACATGTACAACTGATTTCTGCAATGGAAGAAGCATGTAGACAAGTAGAAGAGATGAAAACAAAGGCGAAGAAACTCGAGGAACAGGTGGAGAGGCAAAAAACAGAGATCTTAGAAGCAGCTGAAGAGAAGCGCGAGGCGATCAGGCAACTATGCTTCTCACTTGAGCACTATAGAAATAACTATCATATGCTTAAGCAACATTTTATAGGGCATAAGAGGGTTCCAATTTTGGCTGCCTAA
Protein sequence

>Msa0735550

MAASGVNSSKQMKRLESRKSHSWWWDSHISPKNSKWLFENLEEMDRNVKRMLKLIEEDADSFAKKAEMYYKKRPELVALVEEFYRGYRSLAERYDHVTGELRKNVQSDLQSQGSGFSDTGSEPPSTLPSPNVTHRKSSNRAAGFDFFLGTGGNASDISQKDGEDESSTMTGSEDESDDSSVNNYSAFSRNGTDPGMNRRINELENELREVKEKLWTQEGEHAEVSVSSSGATYENADDVYAKINAYEHELMIVNEKLRLSEEEITKLKTDLENYKSLDSRNMDVGVELEVEGSIDGVDKELFEPSGDTASLGEELRITKENLKASETQVASLKTEVNKSSDRIQQLQDQLDSAKKDVATWKTKFNSEKRESTKLQERLARLKTSLSDRDHEIRDLKTAVSDAEQKIFPEKANLKSEMSKLLEEQTHLKELIREWESRGRSFEEQIRNIQSEKIEMEAELKNGTELLKAEIEQRENSIKDLNVSLDNLKLEKDNLNVEVGSLKEEVNSRDGRIGSLDCHLNELHMEHVQLISAMEEACRQVEEMKTKAKKLEEQVERQKTEILEAAEEKREAIRQLCFSLEHYRNNYHMLKQHFIGHKRVPILAA*