AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0418400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418400 MtrunA17_Chr3g0116141 94.964 139 7 0 1 139 1 139 9.54e-93 264
Msa0418400 MtrunA17_Chr3g0116151 94.964 139 7 0 1 139 2 140 1.08e-92 264
Msa0418400 MtrunA17_Chr4g0057641 73.723 137 36 0 3 139 4 140 6.34e-69 204
Msa0418400 MtrunA17_Chr4g0057651 75.188 133 33 0 6 138 7 139 9.00e-68 201
Msa0418400 MtrunA17_Chr4g0057661 73.529 136 36 0 3 138 4 139 1.22e-67 201
Msa0418400 MtrunA17_Chr6g0459231 55.474 137 61 0 2 138 5 141 1.62e-51 160
Msa0418400 MtrunA17_Chr7g0225921 52.174 138 65 1 2 138 3 140 6.05e-46 146
Msa0418400 MtrunA17_Chr8g0373691 48.148 135 69 1 5 138 53 187 4.76e-35 120
Msa0418400 MtrunA17_Chr7g0245911 41.667 144 74 4 6 139 48 191 3.31e-28 102
Msa0418400 MtrunA17_Chr3g0111371 38.168 131 78 1 9 139 9 136 6.45e-28 100
Msa0418400 MtrunA17_Chr5g0434951 39.394 132 76 2 9 138 8 137 3.30e-27 98.6
Msa0418400 MtrunA17_Chr8g0392951 36.567 134 85 0 6 139 25 158 6.66e-27 98.6
Msa0418400 MtrunA17_Chr1g0161211 38.806 134 81 1 6 138 47 180 1.79e-26 98.2
Msa0418400 MtrunA17_Chr1g0169581 38.346 133 79 1 9 138 8 140 9.07e-26 94.7
Msa0418400 MtrunA17_Chr8g0351681 39.161 143 78 3 6 139 50 192 1.51e-25 95.9
Msa0418400 MtrunA17_Chr8g0391691 38.462 130 79 1 6 134 37 166 7.05e-25 94.0
Msa0418400 MtrunA17_Chr6g0482731 35.821 134 85 1 6 138 27 160 4.95e-22 86.3
Msa0418400 MtrunA17_Chr4g0033601 35.714 140 81 3 6 139 6 142 1.35e-21 84.3
Msa0418400 MtrunA17_Chr5g0402331 34.586 133 86 1 7 138 50 182 2.73e-21 84.7
Msa0418400 MtrunA17_Chr1g0169621 30.380 158 82 2 9 138 8 165 1.01e-20 82.8
Msa0418400 MtrunA17_Chr5g0402361 34.586 133 86 1 7 138 222 354 3.12e-20 84.7
Msa0418400 MtrunA17_Chr5g0402361 34.328 134 84 2 7 138 50 181 2.99e-18 79.3
Msa0418400 MtrunA17_Chr4g0044961 33.846 130 85 1 9 138 61 189 5.89e-20 81.3
Msa0418400 MtrunA17_Chr1g0188171 33.094 139 82 2 6 138 13 146 1.47e-19 79.3
Msa0418400 MtrunA17_Chr7g0254731 30.935 139 85 2 6 138 13 146 2.57e-19 78.6
Msa0418400 MtrunA17_Chr5g0440931 30.216 139 86 2 6 138 13 146 4.56e-18 75.5
Msa0418400 MtrunA17_Chr6g0460211 30.216 139 86 2 6 138 13 146 4.92e-18 75.1
Msa0418400 MtrunA17_Chr1g0166281 33.071 127 84 1 13 138 20 146 6.24e-18 75.1
Msa0418400 MtrunA17_Chr3g0093271 30.496 141 87 2 4 138 62 197 1.31e-17 75.5
Msa0418400 MtrunA17_Chr7g0256171 33.333 135 87 2 8 139 8 142 2.68e-17 73.6
Msa0418400 MtrunA17_Chr7g0256701 33.577 137 85 3 8 138 76 212 6.40e-17 73.9
Msa0418400 MtrunA17_Chr8g0366401 32.090 134 79 2 9 134 21 150 1.65e-16 71.6
Msa0418400 MtrunA17_Chr1g0185001 29.630 135 95 0 4 138 11 145 2.29e-16 70.9
Msa0418400 MtrunA17_Chr5g0396261 34.234 111 70 1 30 137 2 112 9.55e-16 68.6
Msa0418400 MtrunA17_Chr4g0062221 32.331 133 87 2 9 138 15 147 1.32e-15 69.3
Msa0418400 MtrunA17_Chr3g0121191 30.827 133 81 2 7 138 95 217 2.27e-15 70.1
Msa0418400 MtrunA17_Chr7g0245371 32.857 140 81 2 13 139 32 171 3.99e-15 68.2
Msa0418400 MtrunA17_Chr8g0368681 30.827 133 89 2 9 138 16 148 7.14e-15 67.4
Msa0418400 MtrunA17_Chr5g0397101 31.387 137 89 2 5 139 371 504 2.40e-13 65.9
Msa0418400 MtrunA17_Chr8g0387671 30.657 137 90 2 5 139 371 504 3.53e-13 65.5
Msa0418400 MtrunA17_Chr2g0329371 31.884 138 89 2 7 139 66 203 4.21e-13 63.5
Msa0418400 MtrunA17_Chr4g0064231 30.519 154 84 5 7 139 66 217 5.10e-13 63.5
Msa0418400 MtrunA17_Chr6g0450921 31.250 128 87 1 13 139 37 164 7.68e-13 62.0
Msa0418400 MtrunA17_Chr0c02g0489761 27.407 135 97 1 5 139 383 516 1.23e-12 63.9
Msa0418400 MtrunA17_Chr5g0409441 27.273 132 95 1 9 139 26 157 1.76e-12 60.8
Msa0418400 MtrunA17_Chr5g0441691 27.941 136 93 2 5 138 373 505 3.08e-12 62.8
Msa0418400 MtrunA17_Chr7g0245231 30.709 127 87 1 13 138 38 164 3.61e-12 60.5
Msa0418400 MtrunA17_Chr7g0274101 29.851 134 93 1 6 138 13 146 5.19e-12 59.7
Msa0418400 MtrunA17_Chr7g0242561 30.952 126 87 0 14 139 370 495 5.84e-12 62.0
Msa0418400 MtrunA17_Chr5g0444171 29.197 137 92 2 5 139 397 530 1.54e-11 60.5
Msa0418400 MtrunA17_Chr3g0131181 29.197 137 93 2 4 139 333 466 2.45e-11 60.1
Msa0418400 MtrunA17_Chr7g0257751 30.000 140 90 2 4 139 362 497 3.42e-11 59.7
Msa0418400 MtrunA17_Chr1g0173541 28.676 136 97 0 4 139 356 491 3.83e-11 59.3
Msa0418400 MtrunA17_Chr1g0157131 26.277 137 96 2 5 139 379 512 4.30e-11 59.3
Msa0418400 MtrunA17_Chr3g0125311 29.412 153 91 4 1 138 9 159 9.13e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0418400 AT1G76650.1 47.826 138 72 0 2 139 40 177 1.41e-38 129
Msa0418400 AT1G76650.3 47.826 138 72 0 2 139 40 177 1.41e-38 129
Msa0418400 AT1G76640.1 47.552 143 68 3 2 139 19 159 6.74e-36 121
Msa0418400 AT1G76650.2 46.377 138 64 1 2 139 40 167 2.20e-35 120
Msa0418400 AT5G42380.1 45.113 133 71 1 9 139 53 185 1.33e-33 116
Msa0418400 AT3G50770.1 45.985 137 68 2 8 138 67 203 2.54e-33 116
Msa0418400 AT3G01830.1 40.876 137 76 2 6 139 12 146 1.76e-27 99.8
Msa0418400 AT1G24620.1 39.552 134 80 1 6 138 38 171 7.92e-27 99.4
Msa0418400 AT5G37770.1 35.252 139 85 1 6 139 18 156 1.67e-24 92.4
Msa0418400 AT5G37770.2 35.252 139 85 1 6 139 18 156 1.67e-24 92.4
Msa0418400 AT1G66400.1 33.088 136 88 1 6 138 16 151 1.86e-24 92.4
Msa0418400 AT1G18210.2 36.090 133 82 1 6 138 24 153 2.58e-23 89.7
Msa0418400 AT1G18210.1 36.090 133 82 1 6 138 24 153 2.58e-23 89.7
Msa0418400 AT1G73630.1 32.824 131 85 1 9 139 24 151 1.37e-21 85.1
Msa0418400 AT3G07490.1 35.211 142 89 2 1 139 1 142 4.18e-20 80.9
Msa0418400 AT1G05990.1 35.507 138 85 2 6 139 6 143 1.75e-19 79.3
Msa0418400 AT3G59440.1 34.074 135 87 1 7 139 53 187 4.86e-19 79.3
Msa0418400 AT3G22930.1 32.090 134 90 1 6 138 36 169 1.21e-18 77.8
Msa0418400 AT4G03290.1 34.014 147 87 4 1 139 1 145 1.23e-18 77.4
Msa0418400 AT2G15680.1 32.836 134 87 2 7 139 52 183 1.33e-18 78.2
Msa0418400 AT2G43290.1 32.867 143 86 3 6 139 66 207 1.39e-18 78.6
Msa0418400 AT3G56800.1 30.935 139 85 2 6 138 13 146 3.01e-18 76.3
Msa0418400 AT2G41110.1 30.935 139 85 2 6 138 13 146 3.01e-18 76.3
Msa0418400 AT2G27030.1 30.935 139 85 2 6 138 13 146 3.01e-18 76.3
Msa0418400 AT5G21274.1 30.935 139 85 2 6 138 13 146 3.04e-18 76.3
Msa0418400 AT1G66410.1 30.216 139 86 2 6 138 13 146 3.24e-18 76.3
Msa0418400 AT5G37780.1 30.216 139 86 2 6 138 13 146 3.24e-18 76.3
Msa0418400 AT5G17470.1 31.343 134 89 1 9 139 6 139 3.70e-18 75.9
Msa0418400 AT2G27030.3 30.935 139 85 2 6 138 13 146 5.81e-18 76.3
Msa0418400 AT3G43810.1 30.216 139 86 2 6 138 13 146 6.79e-18 75.1
Msa0418400 AT4G14640.1 31.343 134 91 1 6 138 14 147 1.77e-17 74.3
Msa0418400 AT4G12860.1 34.074 135 86 2 8 139 8 142 2.66e-17 73.9
Msa0418400 AT3G10190.1 32.331 133 88 2 8 138 73 205 2.77e-17 75.1
Msa0418400 AT3G43810.2 30.216 139 86 2 6 138 13 146 3.02e-17 75.1
Msa0418400 AT2G41100.7 27.815 151 91 2 6 138 67 217 4.66e-16 73.2
Msa0418400 AT2G41100.6 27.815 151 91 2 6 138 67 217 4.66e-16 73.2
Msa0418400 AT2G41100.3 27.815 151 91 2 6 138 67 217 4.66e-16 73.2
Msa0418400 AT1G18530.1 32.331 133 87 1 9 138 11 143 6.15e-16 70.5
Msa0418400 AT2G41100.4 27.815 151 91 2 6 138 102 252 7.07e-16 72.8
Msa0418400 AT2G41100.4 25.333 150 95 2 6 138 13 162 9.43e-13 64.3
Msa0418400 AT2G41100.1 27.815 151 91 2 6 138 102 252 7.07e-16 72.8
Msa0418400 AT2G41100.1 25.333 150 95 2 6 138 13 162 9.43e-13 64.3
Msa0418400 AT3G50360.1 32.812 128 85 1 13 139 35 162 8.06e-16 70.5
Msa0418400 AT2G41110.2 29.139 151 84 3 6 138 13 158 1.13e-15 69.7
Msa0418400 AT2G36180.1 28.889 135 92 2 9 139 4 138 3.24e-15 68.2
Msa0418400 AT3G03400.1 29.927 137 87 4 1 131 1 134 3.98e-15 67.8
Msa0418400 AT5G37780.2 27.273 154 86 3 6 138 13 161 3.61e-14 65.9
Msa0418400 AT4G23650.1 32.847 137 87 2 5 139 383 516 9.28e-14 67.4
Msa0418400 AT5G12180.1 30.370 135 93 1 5 139 378 511 1.95e-13 66.6
Msa0418400 AT5G37780.4 28.571 126 79 2 19 138 13 133 3.43e-13 62.8
Msa0418400 AT4G14640.2 29.752 121 84 1 19 138 9 129 3.66e-13 62.4
Msa0418400 AT5G19360.1 29.630 135 94 1 5 139 373 506 3.72e-13 65.5
Msa0418400 AT1G66410.2 28.571 126 79 2 19 138 36 156 4.42e-13 62.8
Msa0418400 AT5G37780.3 28.571 126 79 2 19 138 52 172 7.14e-13 62.8
Msa0418400 AT3G51850.1 32.540 126 85 0 14 139 368 493 7.20e-13 64.7
Msa0418400 AT1G66410.4 32.075 106 59 3 41 138 10 110 1.17e-12 60.8
Msa0418400 AT1G66410.3 32.075 106 59 3 41 138 10 110 1.17e-12 60.8
Msa0418400 AT1G32250.1 29.197 137 82 2 9 134 20 152 1.34e-12 62.0
Msa0418400 AT3G43810.4 32.075 106 59 3 41 138 10 110 1.92e-12 60.1
Msa0418400 AT3G43810.3 32.075 106 59 3 41 138 10 110 1.92e-12 60.1
Msa0418400 AT1G76040.1 29.197 137 92 2 5 139 178 311 4.29e-12 62.4
Msa0418400 AT1G76040.4 29.197 137 92 2 5 139 178 311 4.29e-12 62.4
Msa0418400 AT1G76040.2 29.197 137 92 2 5 139 416 549 4.43e-12 62.4
Msa0418400 AT3G25600.1 30.075 133 90 2 9 138 16 148 6.18e-12 60.1
Msa0418400 AT2G41090.1 26.316 133 96 1 6 138 13 143 7.66e-12 60.5
Msa0418400 AT4G37010.2 26.515 132 96 1 9 139 35 166 1.21e-11 59.3
Msa0418400 AT4G37010.1 26.515 132 96 1 9 139 31 162 1.29e-11 59.3
Msa0418400 AT3G51920.1 26.866 134 97 1 6 138 13 146 1.54e-11 58.5
Msa0418400 AT2G41410.1 29.630 135 91 3 8 138 73 207 1.54e-11 59.7
Msa0418400 AT2G31500.1 32.374 139 91 2 4 139 370 508 1.59e-11 60.8
Msa0418400 AT3G03000.1 29.104 134 93 1 4 135 19 152 3.70e-11 58.2
Msa0418400 AT3G10660.2 30.935 139 88 4 4 139 490 623 5.10e-11 59.7
Msa0418400 AT3G10660.1 30.935 139 88 4 4 139 490 623 5.10e-11 59.7
Msa0418400 AT5G12480.1 28.467 137 97 1 4 139 363 499 6.81e-11 59.3
Msa0418400 AT5G12480.2 28.467 137 97 1 4 139 269 405 6.88e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATCCTCTTCAAACTCTT+TGG 0.279285 3_2:+66164622 None:intergenic
GGAGGAATTAATTGCTTTGA+TGG 0.320451 3_2:-66164728 Msa0418400:CDS
TTTGTTGAAAGAAGCTGAAA+TGG 0.376240 3_2:-66164794 Msa0418400:CDS
TGGTGATGGATATTTGAGTT+TGG 0.381496 3_2:-66164749 Msa0418400:CDS
GAAGAGGATGCTTAAGAAGA+TGG 0.388089 3_2:-66164611 Msa0418400:CDS
AGACAAAGGCTGGGAGTAAT+GGG 0.401953 3_2:-66164826 Msa0418400:CDS
AGCCTTTGTCTTAGCTCTGC+TGG 0.405548 3_2:+66164838 None:intergenic
AGCAGAGCTAAGACAAAGGC+TGG 0.426380 3_2:-66164836 Msa0418400:CDS
TTAATTGCTTTGATGGAGGA+AGG 0.458662 3_2:-66164721 Msa0418400:CDS
AAGACAAAGGCTGGGAGTAA+TGG 0.460108 3_2:-66164827 Msa0418400:CDS
AGAAGCTGAAATGGCTATTG+AGG 0.481748 3_2:-66164785 Msa0418400:CDS
AAGAGGATGCTTAAGAAGAT+GGG 0.484014 3_2:-66164610 Msa0418400:CDS
TGGAGAGGAACAAAAGTTGA+AGG 0.498848 3_2:-66164698 Msa0418400:CDS
ATTGCTTTGATGGAGGAAGG+TGG 0.541567 3_2:-66164718 Msa0418400:CDS
TGATGGATATTTGAGTTTGG+AGG 0.566788 3_2:-66164746 Msa0418400:CDS
GGAATTAATTGCTTTGATGG+AGG 0.567051 3_2:-66164725 Msa0418400:CDS
TGAAATGGCTATTGAGGCAA+TGG 0.572267 3_2:-66164779 Msa0418400:CDS
TGATGAAGATGGTGATGGAA+AGG 0.579290 3_2:-66164866 Msa0418400:CDS
TAACACCAAAGAGTTTGAAG+AGG 0.591633 3_2:-66164627 Msa0418400:CDS
CACCAGCAGAGCTAAGACAA+AGG 0.624826 3_2:-66164840 Msa0418400:CDS
GCAGAGCTAAGACAAAGGCT+GGG 0.627677 3_2:-66164835 Msa0418400:CDS
TTTGATGGAGGAAGGTGGAG+AGG 0.629126 3_2:-66164713 Msa0418400:CDS
ATTGAGGCAATGGATTCAGA+TGG 0.638749 3_2:-66164769 Msa0418400:CDS
ATGTATGATAGTGAGAAGTG+TGG 0.644233 3_2:-66164655 Msa0418400:CDS
GCAATGGATTCAGATGGTGA+TGG 0.644671 3_2:-66164763 Msa0418400:CDS
TTATAAAAGATGAAGAACGC+GGG 0.655567 3_2:-66164913 None:intergenic
ATTATAAAAGATGAAGAACG+CGG 0.665781 3_2:-66164914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAACATTTTGATTTGAA+TGG - chr3_2:66164858-66164877 Msa0418400:CDS 15.0%
!!! TTAAACATTTTGATTTGAAT+GGG - chr3_2:66164859-66164878 Msa0418400:CDS 15.0%
!!! TAAACATTTTGATTTGAATG+GGG - chr3_2:66164860-66164879 Msa0418400:CDS 20.0%
!!! CTTAGATATTTTGATGAAGA+TGG - chr3_2:66164534-66164553 Msa0418400:CDS 25.0%
!!! TATTTTGATGAAGATGGTGA+TGG - chr3_2:66164540-66164559 Msa0418400:CDS 30.0%
TTTGTTGAAAGAAGCTGAAA+TGG - chr3_2:66164617-66164636 Msa0418400:CDS 30.0%
!! TGGTGATGGATATTTGAGTT+TGG - chr3_2:66164662-66164681 Msa0418400:CDS 35.0%
! TGATGGATATTTGAGTTTGG+AGG - chr3_2:66164665-66164684 Msa0418400:CDS 35.0%
GGAGGAATTAATTGCTTTGA+TGG - chr3_2:66164683-66164702 Msa0418400:CDS 35.0%
GGAATTAATTGCTTTGATGG+AGG - chr3_2:66164686-66164705 Msa0418400:CDS 35.0%
TTAATTGCTTTGATGGAGGA+AGG - chr3_2:66164690-66164709 Msa0418400:CDS 35.0%
! ATGTATGATAGTGAGAAGTG+TGG - chr3_2:66164756-66164775 Msa0418400:CDS 35.0%
TAACACCAAAGAGTTTGAAG+AGG - chr3_2:66164784-66164803 Msa0418400:CDS 35.0%
AAGAGGATGCTTAAGAAGAT+GGG - chr3_2:66164801-66164820 Msa0418400:CDS 35.0%
!!! CATTTTGATTTGAATGGGGA+TGG - chr3_2:66164864-66164883 Msa0418400:CDS 35.0%
!! TGATGAAGATGGTGATGGAA+AGG - chr3_2:66164545-66164564 Msa0418400:CDS 40.0%
AGAAGCTGAAATGGCTATTG+AGG - chr3_2:66164626-66164645 Msa0418400:CDS 40.0%
TGAAATGGCTATTGAGGCAA+TGG - chr3_2:66164632-66164651 Msa0418400:CDS 40.0%
! ATTGAGGCAATGGATTCAGA+TGG - chr3_2:66164642-66164661 Msa0418400:CDS 40.0%
TGGAGAGGAACAAAAGTTGA+AGG - chr3_2:66164713-66164732 Msa0418400:CDS 40.0%
AGCATCCTCTTCAAACTCTT+TGG + chr3_2:66164792-66164811 None:intergenic 40.0%
GAAGAGGATGCTTAAGAAGA+TGG - chr3_2:66164800-66164819 Msa0418400:CDS 40.0%
AAGACAAAGGCTGGGAGTAA+TGG - chr3_2:66164584-66164603 Msa0418400:CDS 45.0%
AGACAAAGGCTGGGAGTAAT+GGG - chr3_2:66164585-66164604 Msa0418400:CDS 45.0%
! GCAATGGATTCAGATGGTGA+TGG - chr3_2:66164648-66164667 Msa0418400:CDS 45.0%
ATTGCTTTGATGGAGGAAGG+TGG - chr3_2:66164693-66164712 Msa0418400:CDS 45.0%
CACCAGCAGAGCTAAGACAA+AGG - chr3_2:66164571-66164590 Msa0418400:CDS 50.0%
!! AGCCTTTGTCTTAGCTCTGC+TGG + chr3_2:66164576-66164595 None:intergenic 50.0%
AGCAGAGCTAAGACAAAGGC+TGG - chr3_2:66164575-66164594 Msa0418400:CDS 50.0%
GCAGAGCTAAGACAAAGGCT+GGG - chr3_2:66164576-66164595 Msa0418400:CDS 50.0%
TTTGATGGAGGAAGGTGGAG+AGG - chr3_2:66164698-66164717 Msa0418400:CDS 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 66164507 66164926 66164507 ID=Msa0418400;Name=Msa0418400
chr3_2 mRNA 66164507 66164926 66164507 ID=Msa0418400-mRNA-1;Parent=Msa0418400;Name=Msa0418400-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|139
chr3_2 exon 66164507 66164926 66164507 ID=Msa0418400-mRNA-1:exon:15067;Parent=Msa0418400-mRNA-1
chr3_2 CDS 66164507 66164926 66164507 ID=Msa0418400-mRNA-1:cds;Parent=Msa0418400-mRNA-1
Gene Sequence

>Msa0418400

ATGAAGAACGCGGGATTCGAGCATGTTCTTAGATATTTTGATGAAGATGGTGATGGAAAGGTTTCACCAGCAGAGCTAAGACAAAGGCTGGGAGTAATGGGAGAAGAGATTTTGTTGAAAGAAGCTGAAATGGCTATTGAGGCAATGGATTCAGATGGTGATGGATATTTGAGTTTGGAGGAATTAATTGCTTTGATGGAGGAAGGTGGAGAGGAACAAAAGTTGAAGGATTTGAGAGATGCATTTGAGATGTATGATAGTGAGAAGTGTGGATTTATAACACCAAAGAGTTTGAAGAGGATGCTTAAGAAGATGGGTGAGTCTAAGTCTATTGATGAATGTAAAGCTATGATTAAACATTTTGATTTGAATGGGGATGGTGTGCTTAGCTTTGATGAATTCACAGTAATGATGCAGTGA

Protein sequence

>Msa0418400

MKNAGFEHVLRYFDEDGDGKVSPAELRQRLGVMGEEILLKEAEMAIEAMDSDGDGYLSLEELIALMEEGGEEQKLKDLRDAFEMYDSEKCGFITPKSLKRMLKKMGESKSIDECKAMIKHFDLNGDGVLSFDEFTVMMQ*