Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0659790 | XP_003630390.1 | 97.656 | 640 | 15 | 0 | 1 | 640 | 1 | 640 | 0.0 | 1284 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0659790 | sp|Q9XID5|PSI1_ARATH | 56.383 | 658 | 266 | 9 | 1 | 640 | 1 | 655 | 0.0 | 703 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0001830 | Msa0659790 | 0.829779 | 4.252579e-55 | -8.615850e-47 |
| Msa0003630 | Msa0659790 | 0.835082 | 2.063835e-56 | -8.615850e-47 |
| Msa0007850 | Msa0659790 | 0.861909 | 7.365690e-64 | -8.615850e-47 |
| Msa0008200 | Msa0659790 | 0.818259 | 2.151581e-52 | -8.615850e-47 |
| Msa0008220 | Msa0659790 | 0.809325 | 2.001177e-50 | -8.615850e-47 |
| Msa0010490 | Msa0659790 | 0.864354 | 1.291646e-64 | -8.615850e-47 |
| Msa0010500 | Msa0659790 | 0.856258 | 3.632035e-62 | -8.615850e-47 |
| Msa0012710 | Msa0659790 | 0.881898 | 1.635907e-70 | -8.615850e-47 |
| Msa0016540 | Msa0659790 | 0.805379 | 1.373640e-49 | -8.615850e-47 |
| Msa0017320 | Msa0659790 | 0.854674 | 1.051716e-61 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0659790 | MtrunA17_Chr8g0385111 | 97.656 | 640 | 15 | 0 | 1 | 640 | 1 | 640 | 0.0 | 1284 |
| Msa0659790 | MtrunA17_Chr3g0130981 | 71.479 | 568 | 145 | 6 | 73 | 640 | 73 | 623 | 0.0 | 766 |
| Msa0659790 | MtrunA17_Chr1g0200831 | 56.757 | 666 | 240 | 12 | 1 | 637 | 1 | 647 | 0.0 | 697 |
| Msa0659790 | MtrunA17_Chr3g0142751 | 45.908 | 501 | 233 | 7 | 100 | 578 | 76 | 560 | 1.01e-143 | 431 |
| Msa0659790 | MtrunA17_Chr1g0156671 | 49.268 | 410 | 200 | 3 | 105 | 512 | 86 | 489 | 4.31e-126 | 385 |
| Msa0659790 | MtrunA17_Chr1g0188181 | 25.551 | 454 | 257 | 12 | 150 | 531 | 156 | 600 | 3.29e-28 | 119 |
| Msa0659790 | MtrunA17_Chr1g0168081 | 29.187 | 209 | 138 | 2 | 150 | 355 | 43 | 244 | 7.33e-17 | 84.3 |
| Msa0659790 | MtrunA17_Chr1g0168081 | 28.966 | 145 | 90 | 3 | 373 | 513 | 440 | 575 | 1.95e-13 | 73.6 |
| Msa0659790 | MtrunA17_Chr1g0186481 | 27.907 | 215 | 146 | 2 | 150 | 361 | 46 | 254 | 1.47e-16 | 83.2 |
| Msa0659790 | MtrunA17_Chr1g0186481 | 30.137 | 146 | 87 | 3 | 373 | 513 | 410 | 545 | 8.20e-13 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0659790 | AT1G34320.1 | 56.383 | 658 | 266 | 9 | 1 | 640 | 1 | 655 | 0.0 | 703 |
| Msa0659790 | AT1G34320.2 | 56.383 | 658 | 266 | 9 | 1 | 640 | 1 | 655 | 0.0 | 703 |
| Msa0659790 | AT5G08660.3 | 53.385 | 650 | 279 | 9 | 1 | 640 | 1 | 636 | 0.0 | 614 |
| Msa0659790 | AT5G08660.1 | 53.385 | 650 | 279 | 9 | 1 | 640 | 1 | 636 | 0.0 | 614 |
| Msa0659790 | AT5G08660.4 | 53.385 | 650 | 279 | 9 | 1 | 640 | 1 | 636 | 0.0 | 614 |
| Msa0659790 | AT5G08660.2 | 65.423 | 402 | 135 | 2 | 71 | 472 | 74 | 471 | 2.43e-178 | 514 |
| Msa0659790 | AT1G30755.5 | 48.723 | 470 | 228 | 5 | 100 | 562 | 83 | 546 | 7.81e-143 | 429 |
| Msa0659790 | AT1G30755.1 | 48.723 | 470 | 228 | 5 | 100 | 562 | 83 | 546 | 7.81e-143 | 429 |
| Msa0659790 | AT1G30755.6 | 48.723 | 470 | 228 | 5 | 100 | 562 | 83 | 546 | 7.81e-143 | 429 |
| Msa0659790 | AT1G30755.3 | 52.279 | 373 | 175 | 2 | 100 | 471 | 83 | 453 | 9.42e-129 | 387 |
Find 167 sgRNAs with CRISPR-Local
Find 534 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCAATCACAGGAACCT+TGG | 0.539908 | 4_3:-70218124 | None:intergenic |
| AAATGTGACCAGATAATATA+TGG | 0.293418 | 4_3:+70218188 | Msa0659790:exon |
| AACATGAAATGTAGGTAATT+TGG | 0.317265 | 4_3:-70216827 | None:intergenic |
| AACCAATTGGGTGTTGAGTG+TGG | 0.641584 | 4_3:+70210283 | Msa0659790:five_prime_UTR |
| AAGACAAGCTGCTGTTGCTA+AGG | 0.512156 | 4_3:+70210794 | Msa0659790:CDS |
| AAGACAATCAAAGAGACATG+AGG | 0.696981 | 4_3:-70210208 | None:intergenic |
| AATAAAGCCTTTGGCAGTGC+AGG | 0.481849 | 4_3:+70215874 | Msa0659790:CDS |
| AATGTGACCAGATAATATAT+GGG | 0.582045 | 4_3:+70218189 | Msa0659790:exon |
| AATTCAAGTGCAGCTCAAAG+CGG | 0.724106 | 4_3:+70215132 | Msa0659790:CDS |
| AATTGATGCAGAGCGCATCA+CGG | 0.675237 | 4_3:+70217531 | Msa0659790:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTAAGCAAGAAAAACTTGAG+TGG | - | chr4_3:70210479-70210498 | None:intergenic | 30.0% | |
| CAAGTCTACAAAAGGTAATA+AGG | + | chr4_3:70210533-70210552 | Msa0659790:CDS | 30.0% | |
| CACTATGGTAATCATAAATC+TGG | + | chr4_3:70210576-70210595 | Msa0659790:CDS | 30.0% | |
| AGGAATGATGATTTCTATGA+TGG | + | chr4_3:70210720-70210739 | Msa0659790:CDS | 30.0% | |
| GGAATGATGATTTCTATGAT+GGG | + | chr4_3:70210721-70210740 | Msa0659790:CDS | 30.0% | |
| CTATGAAACAAACAAGAGAA+TGG | - | chr4_3:70210834-70210853 | None:intergenic | 30.0% | |
| GTGCTTATGGAAATAGTTTA+TGG | + | chr4_3:70210954-70210973 | Msa0659790:intron | 30.0% | |
| TAATTGATCAAGACCTTGTT+GGG | - | chr4_3:70211119-70211138 | None:intergenic | 30.0% | |
| AAACATATTGTAATTGGCGA+TGG | + | chr4_3:70211176-70211195 | Msa0659790:intron | 30.0% | |
| GATTGAGTTTAGATAGATAG+AGG | + | chr4_3:70211198-70211217 | Msa0659790:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_3 | gene | 70210127 | 70218730 | 70210127 | ID=Msa0659790;Name=Msa0659790 |
| chr4_3 | mRNA | 70210127 | 70218730 | 70210127 | ID=Msa0659790-mRNA-1;Parent=Msa0659790;Name=Msa0659790-mRNA-1;_AED=0.06;_eAED=0.06;_QI=389|1|1|1|1|1|12|527|640 |
| chr4_3 | exon | 70210127 | 70210815 | 70210127 | ID=Msa0659790-mRNA-1:exon:20749;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70213124 | 70213449 | 70213124 | ID=Msa0659790-mRNA-1:exon:20750;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70213552 | 70213644 | 70213552 | ID=Msa0659790-mRNA-1:exon:20751;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70213737 | 70213827 | 70213737 | ID=Msa0659790-mRNA-1:exon:20752;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70215060 | 70215153 | 70215060 | ID=Msa0659790-mRNA-1:exon:20753;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70215512 | 70215609 | 70215512 | ID=Msa0659790-mRNA-1:exon:20754;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70215826 | 70215895 | 70215826 | ID=Msa0659790-mRNA-1:exon:20755;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70216224 | 70216327 | 70216224 | ID=Msa0659790-mRNA-1:exon:20756;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70216742 | 70216858 | 70216742 | ID=Msa0659790-mRNA-1:exon:20757;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70217338 | 70217414 | 70217338 | ID=Msa0659790-mRNA-1:exon:20758;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70217542 | 70217586 | 70217542 | ID=Msa0659790-mRNA-1:exon:20759;Parent=Msa0659790-mRNA-1 |
| chr4_3 | exon | 70217696 | 70218730 | 70217696 | ID=Msa0659790-mRNA-1:exon:20760;Parent=Msa0659790-mRNA-1 |
| chr4_3 | five_prime_UTR | 70210127 | 70210515 | 70210127 | ID=Msa0659790-mRNA-1:five_prime_utr;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70210516 | 70210815 | 70210516 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70213124 | 70213449 | 70213124 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70213552 | 70213644 | 70213552 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70213737 | 70213827 | 70213737 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70215060 | 70215153 | 70215060 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70215512 | 70215609 | 70215512 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70215826 | 70215895 | 70215826 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70216224 | 70216327 | 70216224 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70216742 | 70216858 | 70216742 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70217338 | 70217414 | 70217338 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70217542 | 70217586 | 70217542 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | CDS | 70217696 | 70218203 | 70217696 | ID=Msa0659790-mRNA-1:cds;Parent=Msa0659790-mRNA-1 |
| chr4_3 | three_prime_UTR | 70218204 | 70218730 | 70218204 | ID=Msa0659790-mRNA-1:three_prime_utr;Parent=Msa0659790-mRNA-1 |
| Gene Sequence |
>Msa0659790 ATGGGTGGTCTTTGTTCCAAGTCTACAAAAGGTAATAAGGCTTTAACCAAGAAGGAAGAACACTATGGTAATCATAAATCTGGTGTTGGTAATAAGAAGAATAATCATAAGCATGTTAGTGATTTGACAAGTGCAAAGGAGGGTGTGGATAGTAAGAAAAAGGAAGAAGAAGAAGAAGAAGCTGTTGGTGTTGCTGCAGAAACTAGGAATGATGATTTCTATGATGGGATTCCACGCTATGCTGATTCTTTTTCACATAAGTCTAGGTCAGTAAGATCAAGACAAGCTGCTGTTGCTAAGGTCTCCGAGGTGAGTTCACGCCTGGGCAGAGCTGGCACTATTGGATTCGGGAAAGCAGTTGACGTTCTAGACACACTTGGGAGTAGCATGACAAATTTAAATTCTGGCGGTGGCTTTGCATATGGGGCTGTAACAAAGGGGAACGAAGTTGGTATTTTAGCGTTTGAGGTTGCAAACACTATTGTCAAGGGCTTTAGTCTCATGGAATCTCTTTCAACAAAAAATATTAAACATTTGAAAGAAGAGGTGCTTAAGTTAGAGGCTGTTCAAGATTTAGTATCAAAGGATATGGACGAACTTCTAAGGATTGTTGCTGCAGACAAGAGGGATGAATTGAAAGTATTTTCTGATGAGGTGATTCGTTTTGGAAATCGGTCAAAGGATCCTCAGTGGCACAACTTGGATCGATACTTTGAAAAAATTAGCAGAGAACTTAATTCTCAAAGACAAACAAAAGAAGAGGCAGAGTTATTAATGCAGCAATTGATGAGTTTAGTTCAACATACGGCGGAATTATACCATGAGTTACATGCTTTGGATAGATTCGCACAAGATTATCAGCATAAGCGTGATGAGGAGGACAATTCAAGTGCAGCTCAAAGCGGCGATAGTCTGTCAATCTTGAAGGCAGAACTTAGAAGCCAAAAGAAGCAGGTTAAGCACTTGAAGAAGAAGTCACTCTGGTCCAGAAGTTTGGAGGAGGTTATGGAGAAACTTGTAGACATAGTCCACTTTTTGCATTTGGAGATAAATAAAGCCTTTGGCAGTGCAGATGGCCGTAAACCATTTATTCGAACCATCAGCAATCGCCAAAGATTGGGTCCTGCAGGGCTCTCTTTGCATTATGCAAATATAGTGCTCCAAATGGATACTCTAGTTGCCAGATCCAGTTCTATGCCTGCAAATACAAGGGATACACTATATCAGAGCTTGCCTCCTAATATAAAATTAACTTTGCGCTCCAAATTACCTACATTTCATGTTGCAGAAGAGCTCACTGTAGCAGATATTAAACAGGAGATGGAAAAAACATTGCATTGGTTGGTTCCAATTGCTACAAACACAGCCAAAGCGCATCACGGTTTTGGTTGGGTTGGAGAGTGGGCAAGCACTGGATCTGATCTAAATAAGAAAACTATGAAGACCGAAGTAATGCGGATTGAAACATTTCACCATGCAGATAAAGAAAAGGTAGAAAACTATATTCTTGAACTTCTCTTGTGGCTTCACCGCTTGGCGGTCAAAAGTAAGGCCGGAGGTGATGTTGGAGAAGTCAAGTCTGTCATAAAGTCTCATGTTGGCACTGTCCTGCAAAAGACAAATAAACAATCTACAAACACAGTATCACCATTGCTAACAACCGATGAGCAGATCATGCTGAAGGATGTAAGTAACAAAATTCCTGTTAGAGGGATCATTAAAAGTAAAAGCTTGGACTTTGACAGTCTGAAAATGGAGTTGACAGATAACAGCAAACTGATCAAAAGTAGCAGTTACTCAACGACAAGTAGAAGCAAGGAATTATCTTTTAACAGAATTCACACCAAGGTTCCTGTGATTGATTTTTGTATTGACAAAAAGAGAGCATTGGATGTGATTGACAGAGTAAATGTGACCAGATAA |
| Protein sequence |
>Msa0659790 MGGLCSKSTKGNKALTKKEEHYGNHKSGVGNKKNNHKHVSDLTSAKEGVDSKKKEEEEEEAVGVAAETRNDDFYDGIPRYADSFSHKSRSVRSRQAAVAKVSEVSSRLGRAGTIGFGKAVDVLDTLGSSMTNLNSGGGFAYGAVTKGNEVGILAFEVANTIVKGFSLMESLSTKNIKHLKEEVLKLEAVQDLVSKDMDELLRIVAADKRDELKVFSDEVIRFGNRSKDPQWHNLDRYFEKISRELNSQRQTKEEAELLMQQLMSLVQHTAELYHELHALDRFAQDYQHKRDEEDNSSAAQSGDSLSILKAELRSQKKQVKHLKKKSLWSRSLEEVMEKLVDIVHFLHLEINKAFGSADGRKPFIRTISNRQRLGPAGLSLHYANIVLQMDTLVARSSSMPANTRDTLYQSLPPNIKLTLRSKLPTFHVAEELTVADIKQEMEKTLHWLVPIATNTAKAHHGFGWVGEWASTGSDLNKKTMKTEVMRIETFHHADKEKVENYILELLLWLHRLAVKSKAGGDVGEVKSVIKSHVGTVLQKTNKQSTNTVSPLLTTDEQIMLKDVSNKIPVRGIIKSKSLDFDSLKMELTDNSKLIKSSSYSTTSRSKELSFNRIHTKVPVIDFCIDKKRALDVIDRVNVTR* |
