AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0566030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566030 MtrunA17_Chr8g0380511 99.519 416 2 0 1 416 1 416 0.0 866
Msa0566030 MtrunA17_Chr2g0309251 75.907 386 89 3 33 415 41 425 0.0 611
Msa0566030 MtrunA17_Chr7g0274951 60.154 389 144 8 36 415 53 439 2.70e-174 494
Msa0566030 MtrunA17_Chr1g0189961 58.690 397 146 6 36 415 55 450 9.74e-170 483
Msa0566030 MtrunA17_Chr8g0368161 56.443 388 144 5 32 415 81 447 1.37e-157 452
Msa0566030 MtrunA17_Chr5g0413131 55.238 420 159 7 7 415 61 462 6.82e-156 448
Msa0566030 MtrunA17_Chr4g0058771 56.955 381 146 7 36 415 117 480 3.21e-154 444
Msa0566030 MtrunA17_Chr3g0117271 55.643 381 150 7 36 415 133 495 1.89e-153 443
Msa0566030 MtrunA17_Chr2g0314461 50.649 385 166 8 32 415 44 405 2.29e-129 379
Msa0566030 MtrunA17_Chr8g0368241 49.351 385 176 6 32 415 47 413 2.53e-129 379
Msa0566030 MtrunA17_Chr2g0314451 49.737 380 167 8 37 415 48 404 3.94e-126 370
Msa0566030 MtrunA17_Chr2g0314471 49.870 385 169 8 32 415 44 405 8.47e-125 367
Msa0566030 MtrunA17_Chr6g0453411 42.593 378 186 9 53 413 8 371 4.94e-104 313
Msa0566030 MtrunA17_Chr8g0368201 47.077 325 138 7 111 415 9 319 1.10e-101 305
Msa0566030 MtrunA17_Chr8g0368291 39.767 430 205 12 7 415 8 404 2.75e-99 301
Msa0566030 MtrunA17_Chr3g0135381 38.863 422 238 7 1 411 20 432 4.98e-99 303
Msa0566030 MtrunA17_Chr3g0135371 37.740 416 236 9 10 411 23 429 9.66e-95 291
Msa0566030 MtrunA17_Chr2g0326371 52.667 300 123 8 120 415 75 359 1.31e-93 285
Msa0566030 MtrunA17_Chr8g0368341 42.308 364 175 9 54 415 4 334 4.52e-90 276
Msa0566030 MtrunA17_Chr8g0368181 59.067 193 77 1 223 415 2 192 7.54e-79 242
Msa0566030 MtrunA17_Chr3g0135401 40.252 318 173 5 109 412 32 346 5.12e-76 240
Msa0566030 MtrunA17_Chr8g0368221 44.912 285 122 7 7 290 8 258 6.47e-74 231
Msa0566030 MtrunA17_Chr1g0151871 38.006 321 177 6 98 415 25 326 1.33e-71 228
Msa0566030 MtrunA17_Chr3g0129891 48.214 224 108 4 193 415 163 379 4.50e-69 223
Msa0566030 MtrunA17_Chr3g0129891 56.075 107 37 3 41 146 86 183 8.63e-26 107
Msa0566030 MtrunA17_Chr8g0368361 50.256 195 93 4 223 415 2 194 9.54e-67 211
Msa0566030 MtrunA17_Chr8g0368331 40.072 277 133 5 168 415 13 285 3.27e-66 213
Msa0566030 MtrunA17_Chr8g0368281 48.205 195 99 2 223 416 2 195 2.89e-60 194
Msa0566030 MtrunA17_Chr3g0138811 31.643 414 234 18 9 413 14 387 3.41e-58 195
Msa0566030 MtrunA17_Chr3g0135431 30.890 382 183 9 5 312 12 386 5.70e-54 184
Msa0566030 MtrunA17_Chr3g0135441 37.603 242 120 7 176 411 10 226 4.75e-50 169
Msa0566030 MtrunA17_Chr2g0302691 33.067 375 202 16 51 415 37 372 7.37e-49 170
Msa0566030 MtrunA17_Chr8g0368351 41.126 231 100 6 7 224 41 248 1.29e-47 163
Msa0566030 MtrunA17_Chr8g0339871 40.244 246 133 11 178 415 2 241 2.63e-44 154
Msa0566030 MtrunA17_Chr8g0368231 58.730 126 49 3 291 415 8 131 5.27e-44 149
Msa0566030 MtrunA17_Chr8g0368261 53.077 130 52 3 290 415 4 128 8.47e-41 141
Msa0566030 MtrunA17_Chr8g0368381 49.153 118 58 2 223 339 2 118 4.29e-37 131
Msa0566030 MtrunA17_Chr8g0391201 36.735 245 139 13 179 415 3 239 7.70e-37 134
Msa0566030 MtrunA17_Chr8g0368171 36.232 207 88 7 25 224 35 204 1.18e-30 117
Msa0566030 MtrunA17_Chr5g0445181 27.246 345 186 11 8 352 19 298 1.64e-30 119
Msa0566030 MtrunA17_Chr3g0135451 38.462 156 82 4 5 146 4 159 5.95e-28 109
Msa0566030 MtrunA17_Chr2g0302651 33.778 225 114 11 201 415 1 200 8.66e-26 103
Msa0566030 MtrunA17_Chr8g0353931 29.778 225 125 7 9 224 3 203 1.41e-23 97.8
Msa0566030 MtrunA17_Chr8g0368271 45.045 111 51 3 37 146 52 153 1.89e-21 91.7
Msa0566030 MtrunA17_Chr8g0353971 32.558 172 107 4 243 410 4 170 3.26e-21 90.1
Msa0566030 MtrunA17_Chr8g0353941 33.158 190 116 6 228 413 1 183 3.32e-21 90.5
Msa0566030 MtrunA17_Chr8g0353961 35.878 131 76 3 9 137 3 127 1.24e-20 88.2
Msa0566030 MtrunA17_Chr8g0368321 44.737 114 48 4 34 146 43 142 2.05e-20 88.2
Msa0566030 MtrunA17_Chr5g0426971 29.508 244 137 9 176 413 46 260 6.58e-19 85.9
Msa0566030 MtrunA17_Chr5g0434771 33.125 160 98 5 236 393 12 164 2.94e-18 82.4
Msa0566030 MtrunA17_Chr5g0443801 36.364 110 68 2 179 288 22 129 9.84e-15 72.0
Msa0566030 MtrunA17_Chr3g0135411 61.224 49 19 0 36 84 64 112 2.09e-13 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0566030 AT3G13510.1 81.481 405 74 1 11 415 15 418 0.0 705
Msa0566030 AT1G55360.1 82.353 408 67 4 10 415 17 421 0.0 702
Msa0566030 AT5G56530.2 78.378 407 86 2 10 416 16 420 0.0 678
Msa0566030 AT5G56530.1 78.378 407 86 2 10 416 16 420 0.0 678
Msa0566030 AT2G44210.1 61.165 412 155 5 7 415 5 414 0.0 541
Msa0566030 AT2G44210.2 57.240 442 154 6 7 415 5 444 0.0 528
Msa0566030 AT5G18460.1 58.481 395 153 6 24 415 43 429 5.30e-167 475
Msa0566030 AT1G10750.1 59.474 380 135 6 37 415 105 466 1.04e-163 468
Msa0566030 AT1G23340.2 55.048 416 161 7 8 415 11 408 3.19e-162 462
Msa0566030 AT1G23340.1 55.048 416 161 7 8 415 11 408 3.19e-162 462
Msa0566030 AT1G70550.1 57.368 380 143 6 37 415 103 464 7.07e-159 456
Msa0566030 AT5G50150.1 55.469 384 147 5 36 415 56 419 6.28e-156 447
Msa0566030 AT2G44220.1 51.562 384 173 6 33 415 31 402 5.70e-151 434
Msa0566030 AT2G44240.1 46.602 412 204 7 5 415 5 401 6.14e-133 388
Msa0566030 AT2G17750.1 44.862 399 206 8 18 416 12 396 2.15e-129 379
Msa0566030 AT2G19360.1 45.882 425 203 8 9 415 10 425 1.35e-128 377
Msa0566030 AT2G44250.1 46.081 421 195 11 9 415 6 408 4.38e-122 360
Msa0566030 AT3G48230.1 46.997 383 174 12 41 415 11 372 2.61e-114 339
Msa0566030 AT5G19170.1 43.716 366 181 8 48 410 22 365 1.23e-99 302
Msa0566030 AT1G23340.3 52.577 291 113 6 8 290 11 284 7.67e-99 297
Msa0566030 AT2G44250.2 45.706 326 166 7 97 415 4 325 3.53e-94 286
Msa0566030 AT5G25950.1 41.791 402 201 10 35 415 42 431 7.96e-92 284
Msa0566030 AT2G20170.1 38.499 413 227 13 5 410 4 396 1.51e-88 274
Msa0566030 AT2G20170.2 38.499 413 227 13 5 410 4 396 1.51e-88 274
Msa0566030 AT5G25960.1 37.410 417 185 10 12 415 3 356 2.26e-78 246
Msa0566030 AT2G20170.3 35.593 413 222 13 5 410 4 379 6.70e-77 244
Msa0566030 AT4G23390.1 38.158 380 202 14 41 410 41 397 3.70e-73 234
Msa0566030 AT4G23370.1 35.593 413 236 17 9 410 8 401 6.73e-72 231
Msa0566030 AT4G23380.1 32.282 412 248 11 7 410 10 398 4.99e-68 221
Msa0566030 AT4G23350.1 33.938 386 227 12 33 410 35 400 4.78e-67 219
Msa0566030 AT1G70550.2 53.398 206 78 5 37 241 103 291 9.03e-67 214
Msa0566030 AT4G23365.1 34.536 388 220 13 33 410 35 398 3.78e-66 216
Msa0566030 AT4G23355.1 35.142 387 219 14 33 410 35 398 1.03e-65 215
Msa0566030 AT4G23360.1 32.732 388 228 12 33 410 35 399 1.32e-60 202
Msa0566030 AT1G10750.2 44.635 233 106 7 37 265 105 318 8.05e-57 191
Msa0566030 AT2G35250.1 34.072 361 189 11 58 413 24 340 1.06e-53 182
Msa0566030 AT1G10190.1 32.458 419 236 18 9 410 6 394 5.38e-51 176
Msa0566030 AT4G15050.1 33.249 394 214 19 33 410 34 394 1.00e-49 173
Msa0566030 AT4G15053.1 31.250 416 248 17 9 410 10 401 1.84e-49 172
Msa0566030 AT4G10220.1 29.108 426 254 16 1 410 5 398 3.65e-45 161
Msa0566030 AT4G17505.1 29.620 368 186 11 49 410 18 318 2.17e-42 152
Msa0566030 AT2G27320.1 34.385 317 158 13 60 371 30 301 2.42e-42 152
Msa0566030 AT5G25410.1 28.032 371 227 14 45 410 28 363 8.14e-41 149
Msa0566030 AT2G24950.1 31.008 387 225 17 35 410 54 409 1.95e-40 149
Msa0566030 AT4G23373.1 31.010 287 160 8 9 293 8 258 6.16e-40 145
Msa0566030 AT5G60380.1 28.533 375 218 13 48 410 31 367 1.42e-39 145
Msa0566030 AT5G05030.1 28.232 379 222 16 45 413 22 360 3.37e-37 139
Msa0566030 AT5G46820.1 27.273 374 228 14 48 410 7 347 1.04e-35 135
Msa0566030 AT5G46810.1 26.667 360 233 10 61 410 11 349 2.31e-35 134
Msa0566030 AT4G10210.1 29.723 397 221 18 1 374 5 366 1.44e-34 132
Msa0566030 AT4G23080.1 28.271 428 253 17 5 412 13 406 4.59e-33 129
Msa0566030 AT5G46200.1 25.450 389 237 12 61 410 31 405 2.36e-32 127
Msa0566030 AT4G17860.1 28.297 364 206 16 61 410 29 351 3.10e-32 125
Msa0566030 AT2G38255.1 34.404 218 105 7 61 277 14 194 1.46e-31 120
Msa0566030 AT5G11660.1 30.464 302 183 11 118 413 25 305 7.87e-30 117
Msa0566030 AT5G25415.1 29.927 274 169 10 151 413 72 333 8.37e-25 104
Msa0566030 AT2G03930.2 40.708 113 65 2 147 257 22 134 3.05e-23 95.9
Msa0566030 AT5G36680.1 29.461 241 141 8 118 354 51 266 3.65e-21 94.4
Msa0566030 AT5G36770.1 29.461 241 141 8 118 354 51 266 3.65e-21 94.4
Msa0566030 AT5G37520.1 35.393 178 97 9 240 411 33 198 4.23e-18 82.4
Msa0566030 AT5G04937.1 29.442 197 113 8 220 410 22 198 1.92e-17 80.9

Find 131 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGCCATAGTTGATTAAT+TGG 0.203855 4_1:-66995959 None:intergenic
CTTCTTCCTTCCATATCTTT+TGG 0.227365 4_1:-66996056 None:intergenic
TTGTCTCAGATTTGGGTATT+AGG 0.229770 4_1:+66996500 Msa0566030:CDS
GGGCACTGGTGGATGCAATT+TGG 0.251775 4_1:+66997779 Msa0566030:CDS
TCATTAGTCTGCTGTATCTT+TGG 0.288635 4_1:-66996470 None:intergenic
AGTTCAGTTTGTCTCAGATT+TGG 0.300318 4_1:+66996492 Msa0566030:CDS
TTGGCAAAGCAAGTTACTTT+AGG 0.304436 4_1:+66997963 Msa0566030:CDS
CCTCAAAGATCACAAAATTC+AGG 0.306215 4_1:+66995775 Msa0566030:CDS
TCCTCCTTTGTTCTCCTAAT+AGG 0.309052 4_1:-66996010 None:intergenic
GTATATCCAAGCAACCTTAA+AGG 0.309448 4_1:+66994877 Msa0566030:five_prime_UTR
CAAAAGCTACTATAAATGTT+TGG 0.312208 4_1:+66996444 Msa0566030:CDS
GAGGGAGAGGAGGGGTCATT+AGG 0.313158 4_1:+66994847 Msa0566030:five_prime_UTR
GGCACTGGTGGATGCAATTT+GGG 0.316794 4_1:+66997780 Msa0566030:CDS
CACTTCTCCACTTGCTTACA+TGG 0.336372 4_1:-66998318 None:intergenic
ATCCACCAGTGCCCCTCTTT+CGG 0.340644 4_1:-66997770 None:intergenic
GAATCTCGGTAAGAAGAAAT+TGG 0.342660 4_1:-66996932 None:intergenic
GTGTAAGTGCCAAGTCCTTT+TGG 0.343032 4_1:-66998025 None:intergenic
TTTCTTCTTCTTAGGGTTTG+TGG 0.344075 4_1:+66994908 Msa0566030:five_prime_UTR
CCGAAGGCACGATACCTATT+AGG 0.344482 4_1:+66995996 Msa0566030:CDS
GCAAGTTCAGCCAAAAGATA+TGG 0.346818 4_1:+66996046 Msa0566030:CDS
GAAGAGTGTTATTGTAGTTA+TGG 0.361509 4_1:+66994959 Msa0566030:exon
ACGATATCAGCATACTTATC+TGG 0.363048 4_1:+66996960 Msa0566030:CDS
AAGAGTGTTATTGTAGTTAT+GGG 0.363059 4_1:+66994960 Msa0566030:exon
TGCTACAATCTTCTTTGCTC+AGG 0.364167 4_1:+66996863 Msa0566030:CDS
GTTCAGTTTGTCTCAGATTT+GGG 0.365560 4_1:+66996493 Msa0566030:CDS
AACCTCCCTTTAAGGTTGCT+TGG 0.369446 4_1:-66994883 None:intergenic
GCTTTGCCAAATCCTTCTTC+AGG 0.373471 4_1:-66997950 None:intergenic
GTTGAAGGAGATAAGTTCTA+TGG 0.379108 4_1:+66996419 Msa0566030:CDS
TGTTTCTCCACTTCTCCCTA+TGG 0.381521 4_1:+66998401 Msa0566030:three_prime_UTR
GTTTGAACATCATAACAGTT+TGG 0.388940 4_1:-66998055 None:intergenic
AACAGGTAGTAATGGAGATT+GGG 0.389158 4_1:+66998075 Msa0566030:CDS
TCAACACACTTCAACTCAAA+TGG 0.390051 4_1:+66997913 Msa0566030:CDS
TTGTTATCACCGTATAAATC+TGG 0.391554 4_1:-66996666 None:intergenic
GCCTCCAGTCAAAACCATTC+AGG 0.391742 4_1:+66995115 Msa0566030:CDS
GTACTCCAATTAATCAACTA+TGG 0.396226 4_1:+66995954 Msa0566030:CDS
AAAAGCTACTATAAATGTTT+GGG 0.399911 4_1:+66996445 Msa0566030:CDS
AGCCCTTCAGGATGGAAATT+AGG 0.400463 4_1:-66995890 None:intergenic
AAGGTACCGTGTTGGGATAT+TGG 0.402079 4_1:+66997807 Msa0566030:CDS
CCACTCTGGTTAACCAAATC+AGG 0.405418 4_1:-66996112 None:intergenic
ACACGCGACTATTCACTTAC+TGG 0.406607 4_1:+66996688 Msa0566030:CDS
AAAGCTGGTTGCTTTGAAAC+AGG 0.409468 4_1:-66995740 None:intergenic
GGGAATCAAGGTACCGTGTT+GGG 0.409522 4_1:+66997800 Msa0566030:CDS
TGGGTATTAGGAGGCTCATT+TGG 0.415390 4_1:+66996512 Msa0566030:CDS
TATATCCAAGCAACCTTAAA+GGG 0.419344 4_1:+66994878 Msa0566030:five_prime_UTR
GTTATTGTAGTTATGGGCTG+TGG 0.424545 4_1:+66994966 Msa0566030:exon
CAGGTTAGTCCAGATTTATA+CGG 0.425230 4_1:+66996657 Msa0566030:intron
GAGGTCAGCAGAGCCTGATT+TGG 0.425485 4_1:+66996099 Msa0566030:CDS
CAGAAAGAGAGAGGGAGAGG+AGG 0.426543 4_1:+66994837 Msa0566030:five_prime_UTR
AAGTATGCTGATATCGTATT+GGG 0.427339 4_1:-66996954 None:intergenic
AAACAGGTAGTAATGGAGAT+TGG 0.437514 4_1:+66998074 Msa0566030:CDS
AATCAACTATGGCATGCTAA+TGG 0.441384 4_1:+66995965 Msa0566030:CDS
TAAGTATGCTGATATCGTAT+TGG 0.441706 4_1:-66996955 None:intergenic
GGTTAACCAGAGTGGTCATC+AGG 0.443209 4_1:+66996120 Msa0566030:CDS
AAGCAGAAAGAGAGAGGGAG+AGG 0.444742 4_1:+66994834 Msa0566030:five_prime_UTR
TACAAAGATTGAAGAGAAGA+AGG 0.446224 4_1:-66998222 None:intergenic
GTTGAAGTGTGTTGACCATC+AGG 0.447345 4_1:-66997905 None:intergenic
CCTAATAGGTATCGTGCCTT+CGG 0.450371 4_1:-66995996 None:intergenic
AGGAGGGGTCATTAGGAAGA+AGG 0.450470 4_1:+66994854 Msa0566030:five_prime_UTR
GTCCTCCATAGTAAAAGAAA+TGG 0.450774 4_1:-66998099 None:intergenic
AACTGTTATGATGTTCAAAC+AGG 0.450985 4_1:+66998058 Msa0566030:CDS
CTCAAAGCTCCAAAAGGACT+TGG 0.453716 4_1:+66998016 Msa0566030:CDS
GTTTCTCCACTTCTCCCTAT+GGG 0.453996 4_1:+66998402 Msa0566030:three_prime_UTR
ACAGTGCTACCATGATTGAA+TGG 0.461086 4_1:+66997858 Msa0566030:CDS
CAGTGCTACCATGATTGAAT+GGG 0.467794 4_1:+66997859 Msa0566030:CDS
CAGCATTGAAGCTGGCTGGC+AGG 0.476183 4_1:+66996547 Msa0566030:CDS
TCTTCTTCAACTAGGCTTGG+TGG 0.478443 4_1:+66995044 Msa0566030:CDS
AGAAAGAGAGAGGGAGAGGA+GGG 0.482312 4_1:+66994838 Msa0566030:five_prime_UTR
GTAGTTATGGGCTGTGGGTG+TGG 0.485621 4_1:+66994972 Msa0566030:exon
GGATTTATAATAACACTACT+AGG 0.487493 4_1:-66998201 None:intergenic
TCAAAGAAGCAGAAAGAGAG+AGG 0.488376 4_1:+66994828 Msa0566030:five_prime_UTR
TCTCAGTGTCTTCTTCAACT+AGG 0.498345 4_1:+66995036 Msa0566030:CDS
GTGTCTTCTTCAACTAGGCT+TGG 0.502348 4_1:+66995041 Msa0566030:CDS
GTGTGGTCAAGTTTCTCAAA+AGG 0.503940 4_1:+66998338 Msa0566030:three_prime_UTR
CAACACACTTCAACTCAAAT+GGG 0.505639 4_1:+66997914 Msa0566030:CDS
AATAATCTCAAAGCTCCAAA+AGG 0.510898 4_1:+66998010 Msa0566030:CDS
CAATCTATAATATCCCCATC+TGG 0.512044 4_1:-66995713 None:intergenic
GATGTTCAAACAGGTAGTAA+TGG 0.513582 4_1:+66998067 Msa0566030:CDS
GGAGGGGTCATTAGGAAGAA+GGG 0.515428 4_1:+66994855 Msa0566030:five_prime_UTR
TGGATGCAATTTGGGAATCA+AGG 0.519819 4_1:+66997788 Msa0566030:CDS
TTGTTGTTGCAGAGTCCAGA+TGG 0.520508 4_1:+66995698 Msa0566030:intron
CATGCGATAGCATATGTTGA+AGG 0.521399 4_1:+66996404 Msa0566030:CDS
AGTGATGCATATCAAGCTAC+TGG 0.528855 4_1:+66996839 Msa0566030:CDS
TCTCAGATTTGGGTATTAGG+AGG 0.529071 4_1:+66996503 Msa0566030:CDS
TTCTCCTCCCCATTCAATCA+TGG 0.529304 4_1:-66997867 None:intergenic
GATCTTAACAGCATTGAAGC+TGG 0.532791 4_1:+66996539 Msa0566030:CDS
TGTGATCTTTGAGGAAAGGA+TGG 0.537384 4_1:-66995766 None:intergenic
AGGAAAGGATGGTCAAAAGC+TGG 0.538516 4_1:-66995755 None:intergenic
GTCAGCAGTGACATAGCAAT+GGG 0.541992 4_1:+66996896 Msa0566030:CDS
CTCATTGTAGGATCCGAAAG+AGG 0.542095 4_1:+66997757 Msa0566030:intron
TGGTTCAAGACAGAAGTTTG+AGG 0.544700 4_1:+66995064 Msa0566030:CDS
TGTTGTTGCAGAGTCCAGAT+GGG 0.553616 4_1:+66995699 Msa0566030:intron
AGGATCCGAAAGAGGGGCAC+TGG 0.555253 4_1:+66997765 Msa0566030:intron
GAAAGAGAGAGGGAGAGGAG+GGG 0.555353 4_1:+66994839 Msa0566030:five_prime_UTR
ATCCGAAAGAGGGGCACTGG+TGG 0.556202 4_1:+66997768 Msa0566030:CDS
AAATATGAGAACAAGAAAGA+TGG 0.557574 4_1:-66997830 None:intergenic
CAATGCTTAGTAAGAGTGAG+TGG 0.560405 4_1:+66994790 Msa0566030:five_prime_UTR
TTAACAGCATTGAAGCTGGC+TGG 0.562128 4_1:+66996543 Msa0566030:CDS
TGATATCGTATTGGGAATCT+CGG 0.562866 4_1:-66996946 None:intergenic
AGACCTAATTTCCATCCTGA+AGG 0.567082 4_1:+66995887 Msa0566030:CDS
AACACTCCCATAGGGAGAAG+TGG 0.567702 4_1:-66998408 None:intergenic
TTATTGTAGTTATGGGCTGT+GGG 0.574329 4_1:+66994967 Msa0566030:exon
AGATTCTCCATGTAAGCAAG+TGG 0.578095 4_1:+66998311 Msa0566030:three_prime_UTR
AGTCAGCAGTGACATAGCAA+TGG 0.582074 4_1:+66996895 Msa0566030:CDS
TCATTGTAGGATCCGAAAGA+GGG 0.582114 4_1:+66997758 Msa0566030:intron
TGGGAATCAAGGTACCGTGT+TGG 0.586975 4_1:+66997799 Msa0566030:CDS
AATGCTTAGTAAGAGTGAGT+GGG 0.591240 4_1:+66994791 Msa0566030:five_prime_UTR
ACAGGTAGTAATGGAGATTG+GGG 0.600382 4_1:+66998076 Msa0566030:CDS
ACTTCAACTCAAATGGGAAG+TGG 0.607508 4_1:+66997920 Msa0566030:CDS
GACCTAATTTCCATCCTGAA+GGG 0.607625 4_1:+66995888 Msa0566030:CDS
GCTAATGGTAAATGCTCCGA+AGG 0.608551 4_1:+66995980 Msa0566030:CDS
CAAAGAAGCAGAAAGAGAGA+GGG 0.610664 4_1:+66994829 Msa0566030:five_prime_UTR
GTTGTTAATTCAGAGCCTGA+TGG 0.611724 4_1:+66997890 Msa0566030:CDS
TATCAGCATACTTATCTGGA+AGG 0.613773 4_1:+66996964 Msa0566030:CDS
GGAGAAACAAATAAAATACA+AGG 0.614762 4_1:-66998387 None:intergenic
TATTAGGAGAACAAAGGAGG+AGG 0.617651 4_1:+66996012 Msa0566030:CDS
GTCTTACCTGATGACCACTC+TGG 0.627576 4_1:-66996126 None:intergenic
ATCCAAGCAACCTTAAAGGG+AGG 0.627840 4_1:+66994881 Msa0566030:five_prime_UTR
GTTCAGCCAAAAGATATGGA+AGG 0.639850 4_1:+66996050 Msa0566030:CDS
ACAAGTCGTTCGCAAAGCCG+AGG 0.641777 4_1:+66996080 Msa0566030:CDS
ACCTATTAGGAGAACAAAGG+AGG 0.643991 4_1:+66996009 Msa0566030:CDS
AAATCAGGCTCTGCTGACCT+CGG 0.649895 4_1:-66996097 None:intergenic
CCTGATTTGGTTAACCAGAG+TGG 0.651457 4_1:+66996112 Msa0566030:CDS
ATGCTTAGTAAGAGTGAGTG+GGG 0.659197 4_1:+66994792 Msa0566030:five_prime_UTR
CATTGTAGGATCCGAAAGAG+GGG 0.663178 4_1:+66997759 Msa0566030:intron
GATACCTATTAGGAGAACAA+AGG 0.674157 4_1:+66996006 Msa0566030:CDS
GAGTGTTTGTCAAAATGCAA+TGG 0.684945 4_1:+66998424 Msa0566030:three_prime_UTR
TGTAAGCAAGTGGAGAAGTG+TGG 0.686259 4_1:+66998321 Msa0566030:three_prime_UTR
AGTGCTACCATGATTGAATG+GGG 0.694678 4_1:+66997860 Msa0566030:CDS
GCTACCATGATTGAATGGGG+AGG 0.698683 4_1:+66997863 Msa0566030:CDS
GTTGTTGCAGAGTCCAGATG+GGG 0.710931 4_1:+66995700 Msa0566030:intron
AGATGGCCAATATCCCAACA+CGG 0.758233 4_1:-66997813 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAGTAACTACTTAAAAATA+AGG - chr4_1:66995588-66995607 None:intergenic 15.0%
!!! TTTTTACTAAGTTTATGTTT+TGG + chr4_1:66995853-66995872 Msa0566030:intron 15.0%
!!! AGATAAAAAAACAGTATTTT+AGG - chr4_1:66996793-66996812 None:intergenic 15.0%
!!! ATTAATAATGCAAGAAATTT+TGG - chr4_1:66996999-66997018 None:intergenic 15.0%
!!! TTTTTTTTTTTGACTAATAC+TGG - chr4_1:66997223-66997242 None:intergenic 15.0%
!! ACGTAAAGATAAAAATATTA+GGG - chr4_1:66997505-66997524 None:intergenic 15.0%
!! AACGTAAAGATAAAAATATT+AGG - chr4_1:66997506-66997525 None:intergenic 15.0%
!!! AAATACAAGGATTTTATAAA+GGG - chr4_1:66998377-66998396 None:intergenic 15.0%
!!! AAAATACAAGGATTTTATAA+AGG - chr4_1:66998378-66998397 None:intergenic 15.0%
!!! TTGTTCTTGTTTTTTGTTTA+TGG + chr4_1:66995003-66995022 Msa0566030:CDS 20.0%
!!! TGTTCTTGTTTTTTGTTTAT+GGG + chr4_1:66995004-66995023 Msa0566030:CDS 20.0%
!! AATAAATTAGCTAAAAAGGA+AGG - chr4_1:66995142-66995161 None:intergenic 20.0%
!! GTGAAATAAATTAGCTAAAA+AGG - chr4_1:66995146-66995165 None:intergenic 20.0%
!!! CAAAATAATACTATTTTCTC+TGG - chr4_1:66995223-66995242 None:intergenic 20.0%
!!! TCTTTTCTTTCTTTAATTTG+AGG + chr4_1:66995263-66995282 Msa0566030:intron 20.0%
!!! TTTTTCAGTTCAAAATTGAA+TGG + chr4_1:66995322-66995341 Msa0566030:intron 20.0%
!!! TTTTTTTCCCTTTCAATTTA+GGG - chr4_1:66995459-66995478 None:intergenic 20.0%
!!! TTTTTTTTCCCTTTCAATTT+AGG - chr4_1:66995460-66995479 None:intergenic 20.0%
!! TAAATTGAAAGGGAAAAAAA+AGG + chr4_1:66995459-66995478 Msa0566030:intron 20.0%
!! AAGTAGTTATAAAGAAAAAC+TGG + chr4_1:66995528-66995547 Msa0566030:intron 20.0%
!! AGTAGTTATAAAGAAAAACT+GGG + chr4_1:66995529-66995548 Msa0566030:intron 20.0%
!! AAAAGCTACTATAAATGTTT+GGG + chr4_1:66996445-66996464 Msa0566030:CDS 20.0%
!! AAAAAAAAAAAAAACGTCAG+TGG + chr4_1:66997238-66997257 Msa0566030:intron 20.0%
!! ATCTATAAGATTCATATTAG+CGG - chr4_1:66997422-66997441 None:intergenic 20.0%
!! CGTAAAGATAAAAATATTAG+GGG - chr4_1:66997504-66997523 None:intergenic 20.0%
!!! GAATTTTGTTTGTTAATCAT+AGG - chr4_1:66997578-66997597 None:intergenic 20.0%
!! GAAAAAAAGGAGAAAAAAAA+AGG - chr4_1:66998250-66998269 None:intergenic 20.0%
!!! AGGTTTTTTTCTTCTTCTTA+GGG + chr4_1:66994901-66994920 Msa0566030:five_prime_UTR 25.0%
!!! AAGAGTGTTATTGTAGTTAT+GGG + chr4_1:66994960-66994979 Msa0566030:exon 25.0%
!!! GTTCTTGTTTTTTGTTTATG+GGG + chr4_1:66995005-66995024 Msa0566030:CDS 25.0%
! ACATAAGAAAATGTTCCAAA+GGG - chr4_1:66995169-66995188 None:intergenic 25.0%
! AACATAAGAAAATGTTCCAA+AGG - chr4_1:66995170-66995189 None:intergenic 25.0%
!!! TTTTTTCCCTTTCAATTTAG+GGG - chr4_1:66995458-66995477 None:intergenic 25.0%
! TTCCATAAAACTCATCAAAA+GGG - chr4_1:66995485-66995504 None:intergenic 25.0%
! ATTCCATAAAACTCATCAAA+AGG - chr4_1:66995486-66995505 None:intergenic 25.0%
!!! AGCAAGTTTGGTTTTTATTT+TGG + chr4_1:66995634-66995653 Msa0566030:intron 25.0%
! GATGAAAACAAAGAAAAATC+AGG + chr4_1:66995932-66995951 Msa0566030:CDS 25.0%
!!! ACCATTTTAGTTTCTTCTTT+TGG + chr4_1:66996349-66996368 Msa0566030:intron 25.0%
! ACCAAAAGAAGAAACTAAAA+TGG - chr4_1:66996353-66996372 None:intergenic 25.0%
! CAAAAGCTACTATAAATGTT+TGG + chr4_1:66996444-66996463 Msa0566030:CDS 25.0%
! GGATTTCAATATCAAAATCA+GGG - chr4_1:66996595-66996614 None:intergenic 25.0%
! AGGATTTCAATATCAAAATC+AGG - chr4_1:66996596-66996615 None:intergenic 25.0%
! TAAAACAAAAATGAAAGCCA+CGG - chr4_1:66996749-66996768 None:intergenic 25.0%
!!! TACTCGAATGATTTTTTTAC+TGG + chr4_1:66997277-66997296 Msa0566030:intron 25.0%
! TGCACAATTATAAGAATAAC+AGG - chr4_1:66997461-66997480 None:intergenic 25.0%
! AAATATGAGAACAAGAAAGA+TGG - chr4_1:66997833-66997852 None:intergenic 25.0%
! GGATTTATAATAACACTACT+AGG - chr4_1:66998204-66998223 None:intergenic 25.0%
! GGAGAAACAAATAAAATACA+AGG - chr4_1:66998390-66998409 None:intergenic 25.0%
! TAAAAATCACAACACTAACT+AGG - chr4_1:66998458-66998477 None:intergenic 25.0%
TATATCCAAGCAACCTTAAA+GGG + chr4_1:66994878-66994897 Msa0566030:five_prime_UTR 30.0%
AAGAAAAAAACCTCCCTTTA+AGG - chr4_1:66994894-66994913 None:intergenic 30.0%
!!! GAGGTTTTTTTCTTCTTCTT+AGG + chr4_1:66994900-66994919 Msa0566030:five_prime_UTR 30.0%
!! GAAGAGTGTTATTGTAGTTA+TGG + chr4_1:66994959-66994978 Msa0566030:exon 30.0%
CATAAGAAAATGTTCCAAAG+GGG - chr4_1:66995168-66995187 None:intergenic 30.0%
!! ATTTTGTGATCTTTGAGGAA+AGG - chr4_1:66995773-66995792 None:intergenic 30.0%
GTACTCCAATTAATCAACTA+TGG + chr4_1:66995954-66995973 Msa0566030:CDS 30.0%
! CTTCTTTTGGTCTAATTTTG+TGG + chr4_1:66996362-66996381 Msa0566030:intron 30.0%
TTAGAGTTGTTAGGAAAACA+AGG - chr4_1:66996616-66996635 None:intergenic 30.0%
TTGTTATCACCGTATAAATC+TGG - chr4_1:66996669-66996688 None:intergenic 30.0%
AAGTATGCTGATATCGTATT+GGG - chr4_1:66996957-66996976 None:intergenic 30.0%
TAAGTATGCTGATATCGTAT+TGG - chr4_1:66996958-66996977 None:intergenic 30.0%
!!! TTTTTTTGACTAATACTGGC+AGG - chr4_1:66997219-66997238 None:intergenic 30.0%
!!! AATGATTTTTTTACTGGCAG+TGG + chr4_1:66997283-66997302 Msa0566030:intron 30.0%
CCATGTCATGATAAAGAATT+TGG - chr4_1:66997356-66997375 None:intergenic 30.0%
CCAAATTCTTTATCATGACA+TGG + chr4_1:66997353-66997372 Msa0566030:intron 30.0%
TTGAAGGACTATAAAGAATC+AGG - chr4_1:66997640-66997659 None:intergenic 30.0%
AATAATCTCAAAGCTCCAAA+AGG + chr4_1:66998010-66998029 Msa0566030:CDS 30.0%
GTTTGAACATCATAACAGTT+TGG - chr4_1:66998058-66998077 None:intergenic 30.0%
AACTGTTATGATGTTCAAAC+AGG + chr4_1:66998058-66998077 Msa0566030:CDS 30.0%
! TGACAATTTGCATTTTTACC+AGG - chr4_1:66998124-66998143 None:intergenic 30.0%
TACAAAGATTGAAGAGAAGA+AGG - chr4_1:66998225-66998244 None:intergenic 30.0%
!!! TTTCTCCTTTTTTTCTGTAG+TGG + chr4_1:66998255-66998274 Msa0566030:three_prime_UTR 30.0%
ACTTTCCACTACAGAAAAAA+AGG - chr4_1:66998263-66998282 None:intergenic 30.0%
AATGCTTAGTAAGAGTGAGT+GGG + chr4_1:66994791-66994810 Msa0566030:five_prime_UTR 35.0%
GTATATCCAAGCAACCTTAA+AGG + chr4_1:66994877-66994896 Msa0566030:five_prime_UTR 35.0%
TTTCTTCTTCTTAGGGTTTG+TGG + chr4_1:66994908-66994927 Msa0566030:five_prime_UTR 35.0%
! TTATTGTAGTTATGGGCTGT+GGG + chr4_1:66994967-66994986 Msa0566030:exon 35.0%
GCTAATTTATTTCACCCCTT+TGG + chr4_1:66995151-66995170 Msa0566030:intron 35.0%
GAGGTGATATGTTTCAGAAA+TGG + chr4_1:66995282-66995301 Msa0566030:intron 35.0%
CAAAATTGAATGGCCCTTTA+TGG + chr4_1:66995332-66995351 Msa0566030:intron 35.0%
CTCTTTCCCCTAAATTGAAA+GGG + chr4_1:66995449-66995468 Msa0566030:intron 35.0%
TAGATAGATGCAAGCAAGTT+TGG + chr4_1:66995622-66995641 Msa0566030:intron 35.0%
CAATCTATAATATCCCCATC+TGG - chr4_1:66995716-66995735 None:intergenic 35.0%
! CCTGAATTTTGTGATCTTTG+AGG - chr4_1:66995778-66995797 None:intergenic 35.0%
CCTCAAAGATCACAAAATTC+AGG + chr4_1:66995775-66995794 Msa0566030:CDS 35.0%
GAAACACAGATCTTGAATGA+AGG - chr4_1:66995810-66995829 None:intergenic 35.0%
!! GCTTTTTGAGGAAAACAAGT+TGG + chr4_1:66995910-66995929 Msa0566030:CDS 35.0%
GCATGCCATAGTTGATTAAT+TGG - chr4_1:66995962-66995981 None:intergenic 35.0%
AATCAACTATGGCATGCTAA+TGG + chr4_1:66995965-66995984 Msa0566030:CDS 35.0%
GATACCTATTAGGAGAACAA+AGG + chr4_1:66996006-66996025 Msa0566030:CDS 35.0%
! CTTCTTCCTTCCATATCTTT+TGG - chr4_1:66996059-66996078 None:intergenic 35.0%
AACCAAAACTGTATGCAGTT+CGG - chr4_1:66996266-66996285 None:intergenic 35.0%
! ACTTGTTGAACTGTTAGCTA+CGG + chr4_1:66996295-66996314 Msa0566030:intron 35.0%
GTTGAAGGAGATAAGTTCTA+TGG + chr4_1:66996419-66996438 Msa0566030:CDS 35.0%
! TCATTAGTCTGCTGTATCTT+TGG - chr4_1:66996473-66996492 None:intergenic 35.0%
AGTTCAGTTTGTCTCAGATT+TGG + chr4_1:66996492-66996511 Msa0566030:CDS 35.0%
GTTCAGTTTGTCTCAGATTT+GGG + chr4_1:66996493-66996512 Msa0566030:CDS 35.0%
TTGTCTCAGATTTGGGTATT+AGG + chr4_1:66996500-66996519 Msa0566030:CDS 35.0%
!! AGGAAAGCATTAGAGTTGTT+AGG - chr4_1:66996625-66996644 None:intergenic 35.0%
CTTTCCTCTACATGATTAAC+AGG + chr4_1:66996638-66996657 Msa0566030:intron 35.0%
CTAACCTGTTAATCATGTAG+AGG - chr4_1:66996645-66996664 None:intergenic 35.0%
! CAGGTTAGTCCAGATTTATA+CGG + chr4_1:66996657-66996676 Msa0566030:intron 35.0%
GAATCTCGGTAAGAAGAAAT+TGG - chr4_1:66996935-66996954 None:intergenic 35.0%
TGATATCGTATTGGGAATCT+CGG - chr4_1:66996949-66996968 None:intergenic 35.0%
ACGATATCAGCATACTTATC+TGG + chr4_1:66996960-66996979 Msa0566030:CDS 35.0%
TATCAGCATACTTATCTGGA+AGG + chr4_1:66996964-66996983 Msa0566030:CDS 35.0%
TTGAAAGAATCCAAGCACTT+GGG - chr4_1:66997118-66997137 None:intergenic 35.0%
TTTGAAAGAATCCAAGCACT+TGG - chr4_1:66997119-66997138 None:intergenic 35.0%
GTATCACACATCATACCAAT+TGG - chr4_1:66997157-66997176 None:intergenic 35.0%
CAGGTTCATAAGTGAAGAAA+TGG - chr4_1:66997200-66997219 None:intergenic 35.0%
GTTACGTCTATCAGAATTCA+AGG + chr4_1:66997523-66997542 Msa0566030:intron 35.0%
ACTATAAAGAATCAGGTCGA+TGG - chr4_1:66997633-66997652 None:intergenic 35.0%
AACAGATATCACGACTTCAA+TGG - chr4_1:66997700-66997719 None:intergenic 35.0%
TCAACACACTTCAACTCAAA+TGG + chr4_1:66997913-66997932 Msa0566030:CDS 35.0%
CAACACACTTCAACTCAAAT+GGG + chr4_1:66997914-66997933 Msa0566030:CDS 35.0%
! CATTTTCCTGAAGAAGGATT+TGG + chr4_1:66997944-66997963 Msa0566030:CDS 35.0%
! TTGGCAAAGCAAGTTACTTT+AGG + chr4_1:66997963-66997982 Msa0566030:CDS 35.0%
GATGTTCAAACAGGTAGTAA+TGG + chr4_1:66998067-66998086 Msa0566030:CDS 35.0%
! AAACAGGTAGTAATGGAGAT+TGG + chr4_1:66998074-66998093 Msa0566030:CDS 35.0%
! AACAGGTAGTAATGGAGATT+GGG + chr4_1:66998075-66998094 Msa0566030:CDS 35.0%
GTCCTCCATAGTAAAAGAAA+TGG - chr4_1:66998102-66998121 None:intergenic 35.0%
! TTTTGACAAACACTCCCATA+GGG - chr4_1:66998419-66998438 None:intergenic 35.0%
!! ATTTTGACAAACACTCCCAT+AGG - chr4_1:66998420-66998439 None:intergenic 35.0%
GAGTGTTTGTCAAAATGCAA+TGG + chr4_1:66998424-66998443 Msa0566030:three_prime_UTR 35.0%
CAATGCTTAGTAAGAGTGAG+TGG + chr4_1:66994790-66994809 Msa0566030:five_prime_UTR 40.0%
ATGCTTAGTAAGAGTGAGTG+GGG + chr4_1:66994792-66994811 Msa0566030:five_prime_UTR 40.0%
TCAAAGAAGCAGAAAGAGAG+AGG + chr4_1:66994828-66994847 Msa0566030:five_prime_UTR 40.0%
CAAAGAAGCAGAAAGAGAGA+GGG + chr4_1:66994829-66994848 Msa0566030:five_prime_UTR 40.0%
! GTTATTGTAGTTATGGGCTG+TGG + chr4_1:66994966-66994985 Msa0566030:exon 40.0%
TCTCAGTGTCTTCTTCAACT+AGG + chr4_1:66995036-66995055 Msa0566030:CDS 40.0%
TGGTTCAAGACAGAAGTTTG+AGG + chr4_1:66995064-66995083 Msa0566030:CDS 40.0%
CTAAAAAGGAAGGACCTGAA+TGG - chr4_1:66995132-66995151 None:intergenic 40.0%
CCTTTCAATTTAGGGGAAAG+AGG - chr4_1:66995451-66995470 None:intergenic 40.0%
CCTCTTTCCCCTAAATTGAA+AGG + chr4_1:66995448-66995467 Msa0566030:intron 40.0%
! GGCCCTTTTGATGAGTTTTA+TGG + chr4_1:66995480-66995499 Msa0566030:intron 40.0%
!! AAAGCTGGTTGCTTTGAAAC+AGG - chr4_1:66995743-66995762 None:intergenic 40.0%
! TGTGATCTTTGAGGAAAGGA+TGG - chr4_1:66995769-66995788 None:intergenic 40.0%
AGACCTAATTTCCATCCTGA+AGG + chr4_1:66995887-66995906 Msa0566030:CDS 40.0%
GACCTAATTTCCATCCTGAA+GGG + chr4_1:66995888-66995907 Msa0566030:CDS 40.0%
! TTTTCCTCAAAAAGCCCTTC+AGG - chr4_1:66995905-66995924 None:intergenic 40.0%
ACCTATTAGGAGAACAAAGG+AGG + chr4_1:66996009-66996028 Msa0566030:CDS 40.0%
TCCTCCTTTGTTCTCCTAAT+AGG - chr4_1:66996013-66996032 None:intergenic 40.0%
TATTAGGAGAACAAAGGAGG+AGG + chr4_1:66996012-66996031 Msa0566030:CDS 40.0%
GCAAGTTCAGCCAAAAGATA+TGG + chr4_1:66996046-66996065 Msa0566030:CDS 40.0%
GTTCAGCCAAAAGATATGGA+AGG + chr4_1:66996050-66996069 Msa0566030:CDS 40.0%
GTAAAAACCGATGAGATACG+AGG + chr4_1:66996196-66996215 Msa0566030:intron 40.0%
AACATGACCTCGTATCTCAT+CGG - chr4_1:66996206-66996225 None:intergenic 40.0%
! ATCCGAACTGCATACAGTTT+TGG + chr4_1:66996261-66996280 Msa0566030:intron 40.0%
!!! CTGCATACAGTTTTGGTTCA+TGG + chr4_1:66996268-66996287 Msa0566030:intron 40.0%
CATGCGATAGCATATGTTGA+AGG + chr4_1:66996404-66996423 Msa0566030:CDS 40.0%
TCTCAGATTTGGGTATTAGG+AGG + chr4_1:66996503-66996522 Msa0566030:CDS 40.0%
! GATCTTAACAGCATTGAAGC+TGG + chr4_1:66996539-66996558 Msa0566030:CDS 40.0%
ACAAAAATGAAAGCCACGGA+GGG - chr4_1:66996745-66996764 None:intergenic 40.0%
AACAAAAATGAAAGCCACGG+AGG - chr4_1:66996746-66996765 None:intergenic 40.0%
AGTGATGCATATCAAGCTAC+TGG + chr4_1:66996839-66996858 Msa0566030:CDS 40.0%
TGCTACAATCTTCTTTGCTC+AGG + chr4_1:66996863-66996882 Msa0566030:CDS 40.0%
AGTTGGAACTTGGAAGGATT+AGG - chr4_1:66997062-66997081 None:intergenic 40.0%
ACACATAGTTGGAACTTGGA+AGG - chr4_1:66997068-66997087 None:intergenic 40.0%
TGAAAGAATCCAAGCACTTG+GGG - chr4_1:66997117-66997136 None:intergenic 40.0%
! TCAGTGGAAACCTTTTCTCT+TGG + chr4_1:66997254-66997273 Msa0566030:intron 40.0%
CATTCGAGTACCAAGAGAAA+AGG - chr4_1:66997267-66997286 None:intergenic 40.0%
AGTGTTTCTTCGTTGATCAC+AGG - chr4_1:66997389-66997408 None:intergenic 40.0%
ACGTCTATCAGAATTCAAGG+TGG + chr4_1:66997526-66997545 Msa0566030:intron 40.0%
GAAGCTCAATGGTATGAGTT+GGG - chr4_1:66997666-66997685 None:intergenic 40.0%
TGAAGCTCAATGGTATGAGT+TGG - chr4_1:66997667-66997686 None:intergenic 40.0%
TCTATGAAGGTGAAGCTCAA+TGG - chr4_1:66997677-66997696 None:intergenic 40.0%
ACGACTTCAATGGTCTATGA+AGG - chr4_1:66997690-66997709 None:intergenic 40.0%
TCTCCATTTCCACTCATTGT+AGG + chr4_1:66997745-66997764 Msa0566030:intron 40.0%
GATCCTACAATGAGTGGAAA+TGG - chr4_1:66997751-66997770 None:intergenic 40.0%
TCATTGTAGGATCCGAAAGA+GGG + chr4_1:66997758-66997777 Msa0566030:intron 40.0%
TGGATGCAATTTGGGAATCA+AGG + chr4_1:66997788-66997807 Msa0566030:CDS 40.0%
ACAGTGCTACCATGATTGAA+TGG + chr4_1:66997858-66997877 Msa0566030:CDS 40.0%
CAGTGCTACCATGATTGAAT+GGG + chr4_1:66997859-66997878 Msa0566030:CDS 40.0%
AGTGCTACCATGATTGAATG+GGG + chr4_1:66997860-66997879 Msa0566030:CDS 40.0%
GTTGTTAATTCAGAGCCTGA+TGG + chr4_1:66997890-66997909 Msa0566030:CDS 40.0%
ACTTCAACTCAAATGGGAAG+TGG + chr4_1:66997920-66997939 Msa0566030:CDS 40.0%
!! AGTGGTCATTTTCCTGAAGA+AGG + chr4_1:66997938-66997957 Msa0566030:CDS 40.0%
! ACAGGTAGTAATGGAGATTG+GGG + chr4_1:66998076-66998095 Msa0566030:CDS 40.0%
! TGGGGCCATTTCTTTTACTA+TGG + chr4_1:66998094-66998113 Msa0566030:CDS 40.0%
! GGCCATTTCTTTTACTATGG+AGG + chr4_1:66998097-66998116 Msa0566030:CDS 40.0%
! TTCTTTTACTATGGAGGACC+TGG + chr4_1:66998103-66998122 Msa0566030:CDS 40.0%
AGATTCTCCATGTAAGCAAG+TGG + chr4_1:66998311-66998330 Msa0566030:three_prime_UTR 40.0%
GTGTGGTCAAGTTTCTCAAA+AGG + chr4_1:66998338-66998357 Msa0566030:three_prime_UTR 40.0%
ATCCAAGCAACCTTAAAGGG+AGG + chr4_1:66994881-66994900 Msa0566030:five_prime_UTR 45.0%
!! AACCTCCCTTTAAGGTTGCT+TGG - chr4_1:66994886-66994905 None:intergenic 45.0%
GTGTCTTCTTCAACTAGGCT+TGG + chr4_1:66995041-66995060 Msa0566030:CDS 45.0%
TCTTCTTCAACTAGGCTTGG+TGG + chr4_1:66995044-66995063 Msa0566030:CDS 45.0%
!!! ACCTGAATGGTTTTGACTGG+AGG - chr4_1:66995119-66995138 None:intergenic 45.0%
!!! AGGACCTGAATGGTTTTGAC+TGG - chr4_1:66995122-66995141 None:intergenic 45.0%
CAGCAGAAGCTTGCCATAAA+GGG - chr4_1:66995348-66995367 None:intergenic 45.0%
! TTGTTGTTGCAGAGTCCAGA+TGG + chr4_1:66995698-66995717 Msa0566030:intron 45.0%
TGTTGTTGCAGAGTCCAGAT+GGG + chr4_1:66995699-66995718 Msa0566030:intron 45.0%
AGGAAAGGATGGTCAAAAGC+TGG - chr4_1:66995758-66995777 None:intergenic 45.0%
AGCCCTTCAGGATGGAAATT+AGG - chr4_1:66995893-66995912 None:intergenic 45.0%
GCTAATGGTAAATGCTCCGA+AGG + chr4_1:66995980-66995999 Msa0566030:CDS 45.0%
! CCTAATAGGTATCGTGCCTT+CGG - chr4_1:66995999-66996018 None:intergenic 45.0%
CCACTCTGGTTAACCAAATC+AGG - chr4_1:66996115-66996134 None:intergenic 45.0%
!! CCTGATTTGGTTAACCAGAG+TGG + chr4_1:66996112-66996131 Msa0566030:CDS 45.0%
ATACGAGGTCATGTTCGAGT+TGG + chr4_1:66996211-66996230 Msa0566030:intron 45.0%
ACTGTATGCAGTTCGGATGT+TGG - chr4_1:66996259-66996278 None:intergenic 45.0%
TGGGTATTAGGAGGCTCATT+TGG + chr4_1:66996512-66996531 Msa0566030:CDS 45.0%
! TTAACAGCATTGAAGCTGGC+TGG + chr4_1:66996543-66996562 Msa0566030:CDS 45.0%
ACACGCGACTATTCACTTAC+TGG + chr4_1:66996688-66996707 Msa0566030:CDS 45.0%
ATCACTCTGCAACATGCACA+AGG - chr4_1:66996825-66996844 None:intergenic 45.0%
AGTCAGCAGTGACATAGCAA+TGG + chr4_1:66996895-66996914 Msa0566030:CDS 45.0%
GTCAGCAGTGACATAGCAAT+GGG + chr4_1:66996896-66996915 Msa0566030:CDS 45.0%
TGGAAGGATTAGGAGACATG+TGG - chr4_1:66997052-66997071 None:intergenic 45.0%
CCAGACACATAGTTGGAACT+TGG - chr4_1:66997072-66997091 None:intergenic 45.0%
CCAAGTTCCAACTATGTGTC+TGG + chr4_1:66997069-66997088 Msa0566030:intron 45.0%
CCACATACCAGACACATAGT+TGG - chr4_1:66997079-66997098 None:intergenic 45.0%
CCAACTATGTGTCTGGTATG+TGG + chr4_1:66997076-66997095 Msa0566030:intron 45.0%
AAAGAATCCAAGCACTTGGG+GGG - chr4_1:66997115-66997134 None:intergenic 45.0%
GAAAGAATCCAAGCACTTGG+GGG - chr4_1:66997116-66997135 None:intergenic 45.0%
CCAATTGGAAGAGAGATGCT+AGG - chr4_1:66997142-66997161 None:intergenic 45.0%
CCTAGCATCTCTCTTCCAAT+TGG + chr4_1:66997139-66997158 Msa0566030:intron 45.0%
CTTTCGGATCCTACAATGAG+TGG - chr4_1:66997757-66997776 None:intergenic 45.0%
CTCATTGTAGGATCCGAAAG+AGG + chr4_1:66997757-66997776 Msa0566030:intron 45.0%
CATTGTAGGATCCGAAAGAG+GGG + chr4_1:66997759-66997778 Msa0566030:intron 45.0%
!! AAGGTACCGTGTTGGGATAT+TGG + chr4_1:66997807-66997826 Msa0566030:CDS 45.0%
AGATGGCCAATATCCCAACA+CGG - chr4_1:66997816-66997835 None:intergenic 45.0%
TTCTCCTCCCCATTCAATCA+TGG - chr4_1:66997870-66997889 None:intergenic 45.0%
! GTTGAAGTGTGTTGACCATC+AGG - chr4_1:66997908-66997927 None:intergenic 45.0%
GCTTTGCCAAATCCTTCTTC+AGG - chr4_1:66997953-66997972 None:intergenic 45.0%
CTCAAAGCTCCAAAAGGACT+TGG + chr4_1:66998016-66998035 Msa0566030:CDS 45.0%
! GTGTAAGTGCCAAGTCCTTT+TGG - chr4_1:66998028-66998047 None:intergenic 45.0%
CACTTCTCCACTTGCTTACA+TGG - chr4_1:66998321-66998340 None:intergenic 45.0%
TGTAAGCAAGTGGAGAAGTG+TGG + chr4_1:66998321-66998340 Msa0566030:three_prime_UTR 45.0%
TGTTTCTCCACTTCTCCCTA+TGG + chr4_1:66998401-66998420 Msa0566030:three_prime_UTR 45.0%
GTTTCTCCACTTCTCCCTAT+GGG + chr4_1:66998402-66998421 Msa0566030:three_prime_UTR 45.0%
AAGCAGAAAGAGAGAGGGAG+AGG + chr4_1:66994834-66994853 Msa0566030:five_prime_UTR 50.0%
AGAAAGAGAGAGGGAGAGGA+GGG + chr4_1:66994838-66994857 Msa0566030:five_prime_UTR 50.0%
AGGAGGGGTCATTAGGAAGA+AGG + chr4_1:66994854-66994873 Msa0566030:five_prime_UTR 50.0%
GGAGGGGTCATTAGGAAGAA+GGG + chr4_1:66994855-66994874 Msa0566030:five_prime_UTR 50.0%
GCCTCCAGTCAAAACCATTC+AGG + chr4_1:66995115-66995134 Msa0566030:CDS 50.0%
GCAGCAGAAGCTTGCCATAA+AGG - chr4_1:66995349-66995368 None:intergenic 50.0%
GTTGTTGCAGAGTCCAGATG+GGG + chr4_1:66995700-66995719 Msa0566030:intron 50.0%
CCTCAAAAAGCCCTTCAGGA+TGG - chr4_1:66995901-66995920 None:intergenic 50.0%
! CCATCCTGAAGGGCTTTTTG+AGG + chr4_1:66995898-66995917 Msa0566030:CDS 50.0%
! CCGAAGGCACGATACCTATT+AGG + chr4_1:66995996-66996015 Msa0566030:CDS 50.0%
AAATCAGGCTCTGCTGACCT+CGG - chr4_1:66996100-66996119 None:intergenic 50.0%
GGTTAACCAGAGTGGTCATC+AGG + chr4_1:66996120-66996139 Msa0566030:CDS 50.0%
GTCTTACCTGATGACCACTC+TGG - chr4_1:66996129-66996148 None:intergenic 50.0%
GTTCGGATGTTGGAGCAACT+CGG - chr4_1:66996249-66996268 None:intergenic 50.0%
CACTTGGGGGGACATTACAA+TGG - chr4_1:66997103-66997122 None:intergenic 50.0%
! GGTATGAGTTGGGACGTTGA+AGG - chr4_1:66997656-66997675 None:intergenic 50.0%
!! GGCACTGGTGGATGCAATTT+GGG + chr4_1:66997780-66997799 Msa0566030:CDS 50.0%
!! TGGGAATCAAGGTACCGTGT+TGG + chr4_1:66997799-66997818 Msa0566030:CDS 50.0%
!! GGGAATCAAGGTACCGTGTT+GGG + chr4_1:66997800-66997819 Msa0566030:CDS 50.0%
GCTACCATGATTGAATGGGG+AGG + chr4_1:66997863-66997882 Msa0566030:CDS 50.0%
AACACTCCCATAGGGAGAAG+TGG - chr4_1:66998411-66998430 None:intergenic 50.0%
CAGAAAGAGAGAGGGAGAGG+AGG + chr4_1:66994837-66994856 Msa0566030:five_prime_UTR 55.0%
GAAAGAGAGAGGGAGAGGAG+GGG + chr4_1:66994839-66994858 Msa0566030:five_prime_UTR 55.0%
! GTAGTTATGGGCTGTGGGTG+TGG + chr4_1:66994972-66994991 Msa0566030:exon 55.0%
ACAAGTCGTTCGCAAAGCCG+AGG + chr4_1:66996080-66996099 Msa0566030:CDS 55.0%
GAGGTCAGCAGAGCCTGATT+TGG + chr4_1:66996099-66996118 Msa0566030:CDS 55.0%
AATGAAAGCCACGGAGGGCT+AGG - chr4_1:66996740-66996759 None:intergenic 55.0%
GTAATGTCCCCCCAAGTGCT+TGG + chr4_1:66997105-66997124 Msa0566030:intron 55.0%
ATCCACCAGTGCCCCTCTTT+CGG - chr4_1:66997773-66997792 None:intergenic 55.0%
!! GGGCACTGGTGGATGCAATT+TGG + chr4_1:66997779-66997798 Msa0566030:CDS 55.0%
GAGGGAGAGGAGGGGTCATT+AGG + chr4_1:66994847-66994866 Msa0566030:five_prime_UTR 60.0%
! CAGCATTGAAGCTGGCTGGC+AGG + chr4_1:66996547-66996566 Msa0566030:CDS 60.0%
AAACCATGCCTAGCCCTCCG+TGG + chr4_1:66996729-66996748 Msa0566030:intron 60.0%
! AGGATCCGAAAGAGGGGCAC+TGG + chr4_1:66997765-66997784 Msa0566030:intron 60.0%
!! ATCCGAAAGAGGGGCACTGG+TGG + chr4_1:66997768-66997787 Msa0566030:CDS 60.0%
AAGCCACGGAGGGCTAGGCA+TGG - chr4_1:66996735-66996754 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 66994782 66998502 66994782 ID=Msa0566030;Name=Msa0566030
chr4_1 mRNA 66994782 66998502 66994782 ID=Msa0566030-mRNA-1;Parent=Msa0566030;Name=Msa0566030-mRNA-1;_AED=0.18;_eAED=0.18;_QI=196|1|1|1|1|1|7|355|416
chr4_1 exon 66994782 66995136 66994782 ID=Msa0566030-mRNA-1:exon:21895;Parent=Msa0566030-mRNA-1
chr4_1 exon 66995710 66995796 66995710 ID=Msa0566030-mRNA-1:exon:21896;Parent=Msa0566030-mRNA-1
chr4_1 exon 66995884 66996141 66995884 ID=Msa0566030-mRNA-1:exon:21897;Parent=Msa0566030-mRNA-1
chr4_1 exon 66996404 66996568 66996404 ID=Msa0566030-mRNA-1:exon:21898;Parent=Msa0566030-mRNA-1
chr4_1 exon 66996660 66996713 66996660 ID=Msa0566030-mRNA-1:exon:21899;Parent=Msa0566030-mRNA-1
chr4_1 exon 66996839 66996985 66996839 ID=Msa0566030-mRNA-1:exon:21900;Parent=Msa0566030-mRNA-1
chr4_1 exon 66997767 66998502 66997767 ID=Msa0566030-mRNA-1:exon:21901;Parent=Msa0566030-mRNA-1
chr4_1 five_prime_UTR 66994782 66994977 66994782 ID=Msa0566030-mRNA-1:five_prime_utr;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66994978 66995136 66994978 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66995710 66995796 66995710 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66995884 66996141 66995884 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66996404 66996568 66996404 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66996660 66996713 66996660 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66996839 66996985 66996839 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 CDS 66997767 66998147 66997767 ID=Msa0566030-mRNA-1:cds;Parent=Msa0566030-mRNA-1
chr4_1 three_prime_UTR 66998148 66998502 66998148 ID=Msa0566030-mRNA-1:three_prime_utr;Parent=Msa0566030-mRNA-1
Gene Sequence

>Msa0566030

ATGGGCTGTGGGTGTGGTGTTAGTGTTGTTCTTGTTTTTTGTTTATGGGGTGTTTTGATCTCAGTGTCTTCTTCAACTAGGCTTGGTGGTTCAAGACAGAAGTTTGAGGTTAACAAGCATTTGAATCGTCTTAACAAGCCTCCAGTCAAAACCATTCAGAGTCCAGATGGGGATATTATAGATTGTGTGCCTGTTTCAAAGCAACCAGCTTTTGACCATCCTTTCCTCAAAGATCACAAAATTCAGATGAGACCTAATTTCCATCCTGAAGGGCTTTTTGAGGAAAACAAGTTGGATGAAAACAAAGAAAAATCAGGTACTCCAATTAATCAACTATGGCATGCTAATGGTAAATGCTCCGAAGGCACGATACCTATTAGGAGAACAAAGGAGGAGGATGTTCTAAGAGCAAGTTCAGCCAAAAGATATGGAAGGAAGAAGCACAAGTCGTTCGCAAAGCCGAGGTCAGCAGAGCCTGATTTGGTTAACCAGAGTGGTCATCAGCATGCGATAGCATATGTTGAAGGAGATAAGTTCTATGGAGCAAAAGCTACTATAAATGTTTGGGAACCAAAGATACAGCAGACTAATGAGTTCAGTTTGTCTCAGATTTGGGTATTAGGAGGCTCATTTGGTCAAGATCTTAACAGCATTGAAGCTGGCTGGCAGGTTAGTCCAGATTTATACGGTGATAACAACACGCGACTATTCACTTACTGGACAAGTGATGCATATCAAGCTACTGGTTGCTACAATCTTCTTTGCTCAGGATTTATTCAAGTCAGCAGTGACATAGCAATGGGTGCAAGCATTTCTCCAATTTCTTCTTACCGAGATTCCCAATACGATATCAGCATACTTATCTGGAAGGATCCGAAAGAGGGGCACTGGTGGATGCAATTTGGGAATCAAGGTACCGTGTTGGGATATTGGCCATCTTTCTTGTTCTCATATTTAGCAGACAGTGCTACCATGATTGAATGGGGAGGAGAAGTTGTTAATTCAGAGCCTGATGGTCAACACACTTCAACTCAAATGGGAAGTGGTCATTTTCCTGAAGAAGGATTTGGCAAAGCAAGTTACTTTAGGAATATTCAAGTAGTTGATAGCTCTAATAATCTCAAAGCTCCAAAAGGACTTGGCACTTACACTGAACATCCAAACTGTTATGATGTTCAAACAGGTAGTAATGGAGATTGGGGCCATTTCTTTTACTATGGAGGACCTGGTAAAAATGCAAATTGTCAATGA
Protein sequence

>Msa0566030

MGCGCGVSVVLVFCLWGVLISVSSSTRLGGSRQKFEVNKHLNRLNKPPVKTIQSPDGDIIDCVPVSKQPAFDHPFLKDHKIQMRPNFHPEGLFEENKLDENKEKSGTPINQLWHANGKCSEGTIPIRRTKEEDVLRASSAKRYGRKKHKSFAKPRSAEPDLVNQSGHQHAIAYVEGDKFYGAKATINVWEPKIQQTNEFSLSQIWVLGGSFGQDLNSIEAGWQVSPDLYGDNNTRLFTYWTSDAYQATGCYNLLCSGFIQVSSDIAMGASISPISSYRDSQYDISILIWKDPKEGHWWMQFGNQGTVLGYWPSFLFSYLADSATMIEWGGEVVNSEPDGQHTSTQMGSGHFPEEGFGKASYFRNIQVVDSSNNLKAPKGLGTYTEHPNCYDVQTGSNGDWGHFFYYGGPGKNANCQ*