Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349080 | GAU28263.1 | 86.269 | 335 | 32 | 6 | 6 | 329 | 3 | 334 | 0.0 | 563 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349080 | sp|Q9LJG8|ASIL2_ARATH | 35.772 | 123 | 67 | 2 | 30 | 140 | 61 | 183 | 4.97e-15 | 79.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349080 | A0A2Z6ME36 | 86.269 | 335 | 32 | 6 | 6 | 329 | 3 | 334 | 0.0 | 563 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0349080 | TF | Trihelix |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0349080 | 0.835643 | 1.489099e-56 | -8.615850e-47 |
| Msa0028640 | Msa0349080 | 0.814567 | 1.442362e-51 | -8.615850e-47 |
| Msa0078650 | Msa0349080 | 0.800728 | 1.257480e-48 | -8.615850e-47 |
| Msa0105420 | Msa0349080 | 0.810000 | 1.432890e-50 | -8.615850e-47 |
| Msa0110970 | Msa0349080 | 0.810980 | 8.799247e-51 | -8.615850e-47 |
| Msa0126370 | Msa0349080 | 0.806511 | 7.937614e-50 | -8.615850e-47 |
| Msa0140780 | Msa0349080 | 0.832164 | 1.104818e-55 | -8.615850e-47 |
| Msa0157290 | Msa0349080 | 0.835209 | 1.916693e-56 | -8.615850e-47 |
| Msa0165430 | Msa0349080 | 0.801421 | 9.077061e-49 | -8.615850e-47 |
| Msa0191250 | Msa0349080 | 0.806617 | 7.538933e-50 | -8.615850e-47 |
| Msa0193920 | Msa0349080 | 0.801489 | 8.791831e-49 | -8.615850e-47 |
| Msa0195770 | Msa0349080 | 0.806265 | 8.948114e-50 | -8.615850e-47 |
| Msa0200260 | Msa0349080 | 0.837578 | 4.784469e-57 | -8.615850e-47 |
| Msa0217400 | Msa0349080 | 0.816873 | 4.417553e-52 | -8.615850e-47 |
| Msa0223690 | Msa0349080 | 0.800573 | 1.352986e-48 | -8.615850e-47 |
| Msa0225320 | Msa0349080 | 0.809867 | 1.530483e-50 | -8.615850e-47 |
| Msa0243100 | Msa0349080 | 0.826995 | 1.996829e-54 | -8.615850e-47 |
| Msa0243710 | Msa0349080 | 0.809838 | 1.552605e-50 | -8.615850e-47 |
| Msa0244860 | Msa0349080 | 0.824113 | 9.625742e-54 | -8.615850e-47 |
| Msa0257250 | Msa0349080 | 0.809209 | 2.118207e-50 | -8.615850e-47 |
| Msa0283450 | Msa0349080 | 0.835327 | 1.790327e-56 | -8.615850e-47 |
| Msa0283520 | Msa0349080 | 0.814880 | 1.229991e-51 | -8.615850e-47 |
| Msa0287000 | Msa0349080 | 0.817139 | 3.850083e-52 | -8.615850e-47 |
| Msa0287650 | Msa0349080 | 0.807842 | 4.148016e-50 | -8.615850e-47 |
| Msa0290010 | Msa0349080 | 0.802648 | 5.078008e-49 | -8.615850e-47 |
| Msa0295070 | Msa0349080 | 0.813510 | 2.468320e-51 | -8.615850e-47 |
| Msa0296560 | Msa0349080 | 0.804279 | 2.331262e-49 | -8.615850e-47 |
| Msa0302000 | Msa0349080 | 0.821058 | 4.938871e-53 | -8.615850e-47 |
| Msa0311280 | Msa0349080 | 0.808120 | 3.620385e-50 | -8.615850e-47 |
| Msa0332000 | Msa0349080 | 0.822428 | 2.381103e-53 | -8.615850e-47 |
| Msa0339320 | Msa0349080 | 0.818956 | 1.494900e-52 | -8.615850e-47 |
| Msa0349080 | Msa0366240 | 0.809871 | 1.527103e-50 | -8.615850e-47 |
| Msa0349080 | Msa0388960 | 0.814954 | 1.184277e-51 | -8.615850e-47 |
| Msa0349080 | Msa0407910 | 0.806578 | 7.684350e-50 | -8.615850e-47 |
| Msa0349080 | Msa0410650 | 0.830413 | 2.977649e-55 | -8.615850e-47 |
| Msa0349080 | Msa0417270 | 0.804973 | 1.670338e-49 | -8.615850e-47 |
| Msa0349080 | Msa0417880 | 0.811758 | 5.963272e-51 | -8.615850e-47 |
| Msa0349080 | Msa0427880 | 0.816461 | 5.463901e-52 | -8.615850e-47 |
| Msa0349080 | Msa0428590 | 0.808097 | 3.661155e-50 | -8.615850e-47 |
| Msa0349080 | Msa0428930 | 0.800888 | 1.166469e-48 | -8.615850e-47 |
| Msa0349080 | Msa0466390 | 0.809150 | 2.181238e-50 | -8.615850e-47 |
| Msa0349080 | Msa0474110 | 0.816201 | 6.246554e-52 | -8.615850e-47 |
| Msa0349080 | Msa0491040 | 0.995779 | 8.297057e-220 | -8.615850e-47 |
| Msa0349080 | Msa0507680 | 0.830276 | 3.216801e-55 | -8.615850e-47 |
| Msa0349080 | Msa0509610 | 0.820166 | 7.919767e-53 | -8.615850e-47 |
| Msa0349080 | Msa0514030 | 0.812550 | 4.007028e-51 | -8.615850e-47 |
| Msa0349080 | Msa0518610 | 0.813323 | 2.713128e-51 | -8.615850e-47 |
| Msa0349080 | Msa0524190 | 0.807306 | 5.391372e-50 | -8.615850e-47 |
| Msa0349080 | Msa0532710 | 0.812464 | 4.183530e-51 | -8.615850e-47 |
| Msa0349080 | Msa0540870 | 0.805985 | 1.024814e-49 | -8.615850e-47 |
| Msa0349080 | Msa0546680 | 0.803220 | 3.868625e-49 | -8.615850e-47 |
| Msa0349080 | Msa0566030 | 0.834771 | 2.471444e-56 | -8.615850e-47 |
| Msa0349080 | Msa0574890 | 0.819789 | 9.656206e-53 | -8.615850e-47 |
| Msa0349080 | Msa0606360 | 0.811609 | 6.426102e-51 | -8.615850e-47 |
| Msa0349080 | Msa0623130 | 0.811087 | 8.343309e-51 | -8.615850e-47 |
| Msa0349080 | Msa0647130 | 0.806951 | 6.409786e-50 | -8.615850e-47 |
| Msa0349080 | Msa0647980 | 0.830066 | 3.620505e-55 | -8.615850e-47 |
| Msa0349080 | Msa0657970 | 0.831905 | 1.280645e-55 | -8.615850e-47 |
| Msa0349080 | Msa0754520 | 0.821941 | 3.088285e-53 | -8.615850e-47 |
| Msa0349080 | Msa0757950 | 0.829504 | 4.960921e-55 | -8.615850e-47 |
| Msa0349080 | Msa0769390 | 0.824139 | 9.489090e-54 | -8.615850e-47 |
| Msa0349080 | Msa0789690 | 0.818921 | 1.522702e-52 | -8.615850e-47 |
| Msa0349080 | Msa0801760 | 0.815589 | 8.557118e-52 | -8.615850e-47 |
| Msa0349080 | Msa0810480 | 0.850612 | 1.517420e-60 | -8.615850e-47 |
| Msa0349080 | Msa0829620 | 0.800776 | 1.229733e-48 | -8.615850e-47 |
| Msa0349080 | Msa0832370 | 0.841432 | 4.767630e-58 | -8.615850e-47 |
| Msa0349080 | Msa0833220 | 0.820482 | 6.702658e-53 | -8.615850e-47 |
| Msa0349080 | Msa0844240 | 0.816274 | 6.018496e-52 | -8.615850e-47 |
| Msa0349080 | Msa0867890 | 0.817946 | 2.532867e-52 | -8.615850e-47 |
| Msa0349080 | Msa0869450 | 0.800250 | 1.573875e-48 | -8.615850e-47 |
| Msa0349080 | Msa0870530 | 0.811576 | 6.532415e-51 | -8.615850e-47 |
| Msa0349080 | Msa0880140 | 0.838968 | 2.097426e-57 | -8.615850e-47 |
| Msa0349080 | Msa0908050 | 0.804447 | 2.151022e-49 | -8.615850e-47 |
| Msa0349080 | Msa0909120 | 0.803302 | 3.719688e-49 | -8.615850e-47 |
| Msa0349080 | Msa0990960 | 0.803821 | 2.903199e-49 | -8.615850e-47 |
| Msa0349080 | Msa1008490 | 0.825601 | 4.288933e-54 | -8.615850e-47 |
| Msa0349080 | Msa1069310 | 0.805859 | 1.089096e-49 | -8.615850e-47 |
| Msa0349080 | Msa1082500 | 0.812874 | 3.404341e-51 | -8.615850e-47 |
| Msa0349080 | Msa1083410 | 0.825755 | 3.943776e-54 | -8.615850e-47 |
| Msa0349080 | Msa1085200 | 0.830454 | 2.910838e-55 | -8.615850e-47 |
| Msa0349080 | Msa1098560 | 0.820466 | 6.759421e-53 | -8.615850e-47 |
| Msa0349080 | Msa1104000 | 0.806982 | 6.314548e-50 | -8.615850e-47 |
| Msa0349080 | Msa1144390 | 0.839153 | 1.878658e-57 | -8.615850e-47 |
| Msa0349080 | Msa1151910 | 0.811563 | 6.575684e-51 | -8.615850e-47 |
| Msa0349080 | Msa1179940 | 0.805339 | 1.399878e-49 | -8.615850e-47 |
| Msa0349080 | Msa1179970 | 0.805244 | 1.466025e-49 | -8.615850e-47 |
| Msa0349080 | Msa1201470 | 0.802256 | 6.116007e-49 | -8.615850e-47 |
| Msa0349080 | Msa1215960 | 0.801008 | 1.102560e-48 | -8.615850e-47 |
| Msa0349080 | Msa1222970 | 0.800052 | 1.727313e-48 | -8.615850e-47 |
| Msa0349080 | Msa1228210 | 0.817805 | 2.725150e-52 | -8.615850e-47 |
| Msa0349080 | Msa1240310 | 0.801794 | 7.609946e-49 | -8.615850e-47 |
| Msa0349080 | Msa1249680 | 0.808688 | 2.738646e-50 | -8.615850e-47 |
| Msa0349080 | Msa1269080 | 0.845092 | 5.037336e-59 | -8.615850e-47 |
| Msa0349080 | Msa1270610 | 0.826209 | 3.075280e-54 | -8.615850e-47 |
| Msa0349080 | Msa1271280 | 0.817512 | 3.173720e-52 | -8.615850e-47 |
| Msa0349080 | Msa1271290 | 0.822101 | 2.835500e-53 | -8.615850e-47 |
| Msa0349080 | Msa1271300 | 0.825970 | 3.504891e-54 | -8.615850e-47 |
| Msa0349080 | Msa1278840 | 0.826437 | 2.714894e-54 | -8.615850e-47 |
| Msa0349080 | Msa1283860 | 0.814996 | 1.159110e-51 | -8.615850e-47 |
| Msa0349080 | Msa1295850 | 0.842801 | 2.071022e-58 | -8.615850e-47 |
| Msa0349080 | Msa1308380 | 0.832339 | 1.000433e-55 | -8.615850e-47 |
| Msa0349080 | Msa1313150 | 0.807665 | 4.522967e-50 | -8.615850e-47 |
| Msa0349080 | Msa1318240 | 0.820536 | 6.513332e-53 | -8.615850e-47 |
| Msa0349080 | Msa1329920 | 0.809892 | 1.511490e-50 | -8.615850e-47 |
| Msa0349080 | Msa1365060 | 0.817206 | 3.718585e-52 | -8.615850e-47 |
| Msa0349080 | Msa1367370 | 0.809855 | 1.539319e-50 | -8.615850e-47 |
| Msa0349080 | Msa1392260 | 0.801563 | 8.488570e-49 | -8.615850e-47 |
| Msa0349080 | Msa1409870 | 0.832649 | 8.378314e-56 | -8.615850e-47 |
| Msa0349080 | Msa1410470 | 0.825176 | 5.407077e-54 | -8.615850e-47 |
| Msa0349080 | Msa1423880 | 0.827954 | 1.175826e-54 | -8.615850e-47 |
| Msa0349080 | Msa1429900 | 0.801353 | 9.374152e-49 | -8.615850e-47 |
| Msa0349080 | Msa1431430 | 0.822346 | 2.487753e-53 | -8.615850e-47 |
| Msa0349080 | Msa1438250 | 0.819542 | 1.099438e-52 | -8.615850e-47 |
| Msa0349080 | Msa1443110 | 0.812742 | 3.638011e-51 | -8.615850e-47 |
| Msa0349080 | Msa1447090 | 0.815340 | 9.721431e-52 | -8.615850e-47 |
| Msa0349080 | Msa1460020 | 0.811361 | 7.275312e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349080 | MtrunA17_Chr3g0082261 | 91.071 | 336 | 10 | 7 | 1 | 329 | 1 | 323 | 0.0 | 553 |
| Msa0349080 | MtrunA17_Chr1g0164461 | 89.447 | 199 | 5 | 6 | 1 | 196 | 1 | 186 | 4.28e-110 | 318 |
| Msa0349080 | MtrunA17_Chr1g0164431 | 92.929 | 99 | 3 | 1 | 235 | 329 | 1 | 99 | 8.61e-57 | 179 |
| Msa0349080 | MtrunA17_Chr8g0344681 | 24.922 | 321 | 165 | 11 | 26 | 309 | 52 | 333 | 1.66e-17 | 82.4 |
| Msa0349080 | MtrunA17_Chr2g0283871 | 36.290 | 124 | 71 | 4 | 40 | 156 | 18 | 140 | 1.55e-16 | 79.0 |
| Msa0349080 | MtrunA17_Chr1g0179531 | 40.816 | 98 | 49 | 3 | 39 | 130 | 127 | 221 | 2.22e-15 | 76.6 |
| Msa0349080 | MtrunA17_Chr4g0041151 | 23.077 | 286 | 178 | 7 | 43 | 309 | 29 | 291 | 1.41e-13 | 70.5 |
| Msa0349080 | MtrunA17_Chr5g0402321 | 33.036 | 112 | 66 | 3 | 45 | 147 | 22 | 133 | 5.26e-12 | 64.7 |
| Msa0349080 | MtrunA17_Chr4g0066271 | 32.673 | 101 | 61 | 1 | 39 | 132 | 111 | 211 | 5.51e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0349080 | AT3G54390.1 | 59.386 | 293 | 89 | 6 | 38 | 328 | 31 | 295 | 4.68e-102 | 302 |
| Msa0349080 | AT3G54390.2 | 59.386 | 293 | 89 | 6 | 38 | 328 | 47 | 311 | 6.83e-102 | 302 |
| Msa0349080 | AT3G10030.1 | 42.000 | 100 | 51 | 2 | 33 | 127 | 149 | 246 | 1.54e-17 | 83.6 |
| Msa0349080 | AT3G10030.2 | 40.777 | 103 | 54 | 2 | 33 | 130 | 149 | 249 | 1.99e-17 | 83.2 |
| Msa0349080 | AT3G14180.1 | 35.772 | 123 | 67 | 2 | 30 | 140 | 61 | 183 | 5.05e-16 | 79.0 |
| Msa0349080 | AT3G58630.2 | 25.773 | 291 | 175 | 10 | 41 | 309 | 22 | 293 | 2.21e-14 | 73.2 |
| Msa0349080 | AT3G58630.1 | 25.773 | 291 | 175 | 10 | 41 | 309 | 22 | 293 | 2.64e-14 | 73.2 |
| Msa0349080 | AT3G24860.1 | 40.698 | 86 | 47 | 2 | 45 | 129 | 65 | 147 | 6.45e-14 | 71.6 |
| Msa0349080 | AT3G11100.1 | 43.333 | 90 | 49 | 2 | 41 | 130 | 18 | 105 | 8.97e-13 | 67.8 |
| Msa0349080 | AT2G44730.1 | 33.673 | 98 | 58 | 1 | 39 | 129 | 58 | 155 | 1.21e-11 | 65.5 |
Find 65 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCATCTTCTCTATCTCTTTC+AGG | 0.111026 | 3_1:-12295146 | None:intergenic |
| AAACAAAACAATATTATTAT+TGG | 0.212537 | 3_1:+12293097 | Msa0349080:CDS |
| TTACACTGTGTTTGTGTCTT+TGG | 0.214518 | 3_1:-12293295 | None:intergenic |
| TTGAAGCATATGAATCAAAA+TGG | 0.232049 | 3_1:+12293185 | Msa0349080:CDS |
| TAATTGACTTTAATTGTAGA+TGG | 0.322551 | 3_1:+12295419 | Msa0349080:three_prime_UTR |
| ATGGATTCTTGTTAGCTATA+TGG | 0.326116 | 3_1:-12294939 | None:intergenic |
| TTCAATTAATTAACAAATGA+AGG | 0.338320 | 3_1:+12295459 | Msa0349080:three_prime_UTR |
| CATCTTCTCTATCTCTTTCA+GGG | 0.360575 | 3_1:-12295145 | None:intergenic |
| AGAGAGAGCCACTAGCATTA+TGG | 0.366176 | 3_1:-12292809 | None:intergenic |
| ATCTACAATTAAAGTCAATT+AGG | 0.382284 | 3_1:-12295417 | None:intergenic |
| GTTGATTCATCATTGAGAAT+TGG | 0.394877 | 3_1:+12295285 | Msa0349080:CDS |
| TGGTCATCTCTTCACTAGTT+AGG | 0.401307 | 3_1:-12295372 | None:intergenic |
| GTAGATGGAGGATATTATTA+TGG | 0.406791 | 3_1:+12292993 | Msa0349080:five_prime_UTR |
| ACTAAACAATAATCAAGGTT+TGG | 0.408425 | 3_1:+12293444 | Msa0349080:CDS |
| ATTATTATTGGCAGTGGAAG+TGG | 0.409807 | 3_1:+12293109 | Msa0349080:CDS |
| AACAATATTATTATTGGCAG+TGG | 0.416431 | 3_1:+12293103 | Msa0349080:CDS |
| AAGCGCCGGCGTGCTGCTAT+CGG | 0.427506 | 3_1:-12294968 | None:intergenic |
| AGATTATAATTATTGTCATA+TGG | 0.428312 | 3_1:-12295392 | None:intergenic |
| CAAAACTCAAAGGACAAGAT+TGG | 0.436569 | 3_1:+12293224 | Msa0349080:CDS |
| GGTGATTCTTGTTGTGATGT+TGG | 0.438336 | 3_1:-12293070 | None:intergenic |
| CAAAACTCACATGGATCAAA+TGG | 0.444369 | 3_1:+12293565 | Msa0349080:CDS |
| CTAGTTCAGATGTTTCATCT+TGG | 0.449491 | 3_1:+12293362 | Msa0349080:CDS |
| TGAGGCAAAGAGAGGAGAAA+TGG | 0.461965 | 3_1:+12295179 | Msa0349080:CDS |
| GGTGGAGGAACAACCGTACT+AGG | 0.467128 | 3_1:-12293529 | None:intergenic |
| GACTTGATCTTCTTCTTCGT+GGG | 0.486660 | 3_1:+12293398 | Msa0349080:CDS |
| GATCTTCTTCTTCGTGGGAC+AGG | 0.486736 | 3_1:+12293403 | Msa0349080:CDS |
| AAAACTCAAAGGACAAGATT+GGG | 0.491011 | 3_1:+12293225 | Msa0349080:CDS |
| TGGGAATAGCAGAAAGCATA+AGG | 0.497144 | 3_1:+12295076 | Msa0349080:CDS |
| TGAAGCATATGAATCAAAAT+GGG | 0.499206 | 3_1:+12293186 | Msa0349080:CDS |
| AGACTTGATCTTCTTCTTCG+TGG | 0.502001 | 3_1:+12293397 | Msa0349080:CDS |
| ATTCTTGTTGTGATGTTGGA+GGG | 0.502379 | 3_1:-12293066 | None:intergenic |
| TTGTTAATTAATTGAAGAGA+AGG | 0.502593 | 3_1:-12295452 | None:intergenic |
| CATGAGGCTAAGAATATGAA+AGG | 0.514172 | 3_1:-12292903 | None:intergenic |
| GAGGCAAAAGAAAGAAACAA+TGG | 0.514233 | 3_1:+12295056 | Msa0349080:CDS |
| TGAGCAGTTGTAATTGGTGG+AGG | 0.515009 | 3_1:-12293544 | None:intergenic |
| AGATTGAGTCCATGAAGAAA+AGG | 0.517431 | 3_1:+12293323 | Msa0349080:CDS |
| GGCTAGCAGAGGTGGTGGTA+AGG | 0.529819 | 3_1:+12295100 | Msa0349080:CDS |
| AGGCAAAAGAAAGAAACAAT+GGG | 0.536131 | 3_1:+12295057 | Msa0349080:CDS |
| TATAGCTAACAAGAATCCAT+TGG | 0.536715 | 3_1:+12294942 | Msa0349080:CDS |
| AAGGTCAGAACAAACAAGAA+TGG | 0.538536 | 3_1:+12295119 | Msa0349080:CDS |
| GATTCTTGTTGTGATGTTGG+AGG | 0.544639 | 3_1:-12293067 | None:intergenic |
| TCAATTAATTAACAAATGAA+GGG | 0.550010 | 3_1:+12295460 | Msa0349080:three_prime_UTR |
| CAGAGAACAATAACAACAAG+AGG | 0.552289 | 3_1:+12295037 | Msa0349080:CDS |
| ACAACACTAAACAATAATCA+AGG | 0.555763 | 3_1:+12293439 | Msa0349080:CDS |
| ATAAAATTCCATAATGCTAG+TGG | 0.556361 | 3_1:+12292801 | Msa0349080:five_prime_UTR |
| ATACACAACTAGAGATTGCA+AGG | 0.568347 | 3_1:+12295229 | Msa0349080:CDS |
| CATAAGGTGGCTAGCAGAGG+TGG | 0.573732 | 3_1:+12295092 | Msa0349080:CDS |
| GAAGATGAGAGTTGAAGCTG+AGG | 0.581651 | 3_1:+12295161 | Msa0349080:CDS |
| AAGCATAAGGTGGCTAGCAG+AGG | 0.587151 | 3_1:+12295089 | Msa0349080:CDS |
| GTGCTGCTATCGGTTTCCAA+TGG | 0.590073 | 3_1:-12294958 | None:intergenic |
| AAAAGAGATGAATGGAGTGA+AGG | 0.593095 | 3_1:+12293145 | Msa0349080:CDS |
| AAGGTGGCTAGCAGAGGTGG+TGG | 0.597138 | 3_1:+12295095 | Msa0349080:CDS |
| ATGAGGCTAAGAATATGAAA+GGG | 0.599291 | 3_1:-12292902 | None:intergenic |
| AGAAATAGAGCAAAACTCAA+AGG | 0.604235 | 3_1:+12293214 | Msa0349080:CDS |
| GTTGAAGCTGAGGCAAAGAG+AGG | 0.612939 | 3_1:+12295171 | Msa0349080:CDS |
| ACAACTGCTCAAAACTCACA+TGG | 0.614879 | 3_1:+12293556 | Msa0349080:CDS |
| TGGTGTTGATAAATTAGTCA+AGG | 0.628166 | 3_1:+12293585 | Msa0349080:CDS |
| TGGAAGAAGCTAATGATATG+TGG | 0.630070 | 3_1:+12295305 | Msa0349080:exon |
| TCAGCCACTAGCTCCTAGTA+CGG | 0.644324 | 3_1:+12293516 | Msa0349080:CDS |
| GGAAACCGATAGCAGCACGC+CGG | 0.658154 | 3_1:+12294963 | Msa0349080:CDS |
| ACAACCGTACTAGGAGCTAG+TGG | 0.669765 | 3_1:-12293520 | None:intergenic |
| AGATCAAGTCTAGAATACAA+AGG | 0.687202 | 3_1:-12293385 | None:intergenic |
| GAATAGCAGAAAGCATAAGG+TGG | 0.715377 | 3_1:+12295079 | Msa0349080:CDS |
| GCATGTGACATAACAAGTGG+TGG | 0.722377 | 3_1:-12293478 | None:intergenic |
| TGTGCATGTGACATAACAAG+TGG | 0.766342 | 3_1:-12293481 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTTTATTTTATTTTT+AGG | + | chr3_1:12293751-12293770 | Msa0349080:intron | 0.0% |
| !! | AAAAAATAAAAAAAATAAAT+TGG | - | chr3_1:12293995-12294014 | None:intergenic | 0.0% |
| !! | AATAAAATAATTTATATATT+CGG | + | chr3_1:12294372-12294391 | Msa0349080:intron | 0.0% |
| !! | AAACAAAACAATATTATTAT+TGG | + | chr3_1:12293097-12293116 | Msa0349080:CDS | 10.0% |
| !! | AGACTTAAAAAAAATAATTA+TGG | + | chr3_1:12293792-12293811 | Msa0349080:intron | 10.0% |
| !!! | ATTTATTTTAAAGAAATTGA+TGG | + | chr3_1:12294211-12294230 | Msa0349080:intron | 10.0% |
| !!! | TTTAAATATTGATTGTATAA+AGG | + | chr3_1:12294658-12294677 | Msa0349080:intron | 10.0% |
| !!! | ATAATATTGTTTTGTTTTCT+TGG | - | chr3_1:12293094-12293113 | None:intergenic | 15.0% |
| !! | TTGTTAAATATTAAAGTCAA+AGG | + | chr3_1:12293963-12293982 | Msa0349080:intron | 15.0% |
| !! | ATAATAATCAAATTGTTAAG+TGG | + | chr3_1:12294402-12294421 | Msa0349080:intron | 15.0% |
| !! | TAATAATCAAATTGTTAAGT+GGG | + | chr3_1:12294403-12294422 | Msa0349080:intron | 15.0% |
| !! | TTAATGCATATATAGTATAA+TGG | - | chr3_1:12294442-12294461 | None:intergenic | 15.0% |
| !! | TAGTATATGGTTAAAATAAA+TGG | - | chr3_1:12294490-12294509 | None:intergenic | 15.0% |
| !!! | TTTTTTTTCTTTTCTTTTGA+TGG | - | chr3_1:12294631-12294650 | None:intergenic | 15.0% |
| !! | AGATTATAATTATTGTCATA+TGG | - | chr3_1:12295395-12295414 | None:intergenic | 15.0% |
| !! | TTCAATTAATTAACAAATGA+AGG | + | chr3_1:12295459-12295478 | Msa0349080:three_prime_UTR | 15.0% |
| !! | TCAATTAATTAACAAATGAA+GGG | + | chr3_1:12295460-12295479 | Msa0349080:three_prime_UTR | 15.0% |
| !! | GTTAAAAAGTTTATAGTAGA+TGG | + | chr3_1:12292978-12292997 | Msa0349080:five_prime_UTR | 20.0% |
| !!! | TATTTTTAGGACTAAAAAGT+AGG | + | chr3_1:12293764-12293783 | Msa0349080:intron | 20.0% |
| !! | AAAAACAATGATCTTCATAT+AGG | - | chr3_1:12294122-12294141 | None:intergenic | 20.0% |
| !!! | AAGATCATTGTTTTTTTTGA+AGG | + | chr3_1:12294127-12294146 | Msa0349080:intron | 20.0% |
| !!! | AGATCATTGTTTTTTTTGAA+GGG | + | chr3_1:12294128-12294147 | Msa0349080:intron | 20.0% |
| !! | ATATAATCTAAAGAAATGTG+AGG | + | chr3_1:12294180-12294199 | Msa0349080:intron | 20.0% |
| !! | TAAACATGCATAATAGTATA+TGG | - | chr3_1:12294503-12294522 | None:intergenic | 20.0% |
| !! | AAAAAAATTAGTGCATATGT+TGG | + | chr3_1:12294525-12294544 | Msa0349080:intron | 20.0% |
| !!! | GTTTTATATGATTTTACCAT+GGG | - | chr3_1:12294696-12294715 | None:intergenic | 20.0% |
| !!! | AGTTTTATATGATTTTACCA+TGG | - | chr3_1:12294697-12294716 | None:intergenic | 20.0% |
| !! | TAGTAAACTCAAATATCTTT+GGG | - | chr3_1:12294742-12294761 | None:intergenic | 20.0% |
| !! | ATAGTAAACTCAAATATCTT+TGG | - | chr3_1:12294743-12294762 | None:intergenic | 20.0% |
| !! | GATAGCGAAATATATATAAT+TGG | - | chr3_1:12294796-12294815 | None:intergenic | 20.0% |
| !! | ATTTATGATTTCATATTGAC+AGG | + | chr3_1:12294888-12294907 | Msa0349080:intron | 20.0% |
| !! | ATCTACAATTAAAGTCAATT+AGG | - | chr3_1:12295420-12295439 | None:intergenic | 20.0% |
| !!! | TAATTGACTTTAATTGTAGA+TGG | + | chr3_1:12295419-12295438 | Msa0349080:three_prime_UTR | 20.0% |
| !! | TTGTTAATTAATTGAAGAGA+AGG | - | chr3_1:12295455-12295474 | None:intergenic | 20.0% |
| ! | AAGAATGGCTATGAAAAAAA+TGG | - | chr3_1:12292751-12292770 | None:intergenic | 25.0% |
| ! | AAAAAGGGAAAAGAAAAGAA+TGG | - | chr3_1:12292766-12292785 | None:intergenic | 25.0% |
| ! | ATAAAATTCCATAATGCTAG+TGG | + | chr3_1:12292801-12292820 | Msa0349080:five_prime_UTR | 25.0% |
| ! | AAAAAGTTTATAGTAGATGG+AGG | + | chr3_1:12292981-12293000 | Msa0349080:five_prime_UTR | 25.0% |
| ! | AACAATATTATTATTGGCAG+TGG | + | chr3_1:12293103-12293122 | Msa0349080:CDS | 25.0% |
| ! | TTGAAGCATATGAATCAAAA+TGG | + | chr3_1:12293185-12293204 | Msa0349080:CDS | 25.0% |
| ! | TGAAGCATATGAATCAAAAT+GGG | + | chr3_1:12293186-12293205 | Msa0349080:CDS | 25.0% |
| ! | ACAACACTAAACAATAATCA+AGG | + | chr3_1:12293439-12293458 | Msa0349080:CDS | 25.0% |
| ! | ACTAAACAATAATCAAGGTT+TGG | + | chr3_1:12293444-12293463 | Msa0349080:CDS | 25.0% |
| !! | AAGCAATTTTGTAATTTGGA+AGG | - | chr3_1:12293711-12293730 | None:intergenic | 25.0% |
| !! | GAACAAGCAATTTTGTAATT+TGG | - | chr3_1:12293715-12293734 | None:intergenic | 25.0% |
| ! | ATTACAAAATTGCTTGTTCA+AGG | + | chr3_1:12293716-12293735 | Msa0349080:intron | 25.0% |
| ! | AAAACTCACGACTAAAAATA+AGG | + | chr3_1:12293886-12293905 | Msa0349080:intron | 25.0% |
| ! | TTTCACTCACATAATTAAGT+AGG | - | chr3_1:12294083-12294102 | None:intergenic | 25.0% |
| ! | GCAATAAGAAATTCATTTGA+AGG | - | chr3_1:12294309-12294328 | None:intergenic | 25.0% |
| !! | TGATTGTATAAAGGTACAAA+AGG | + | chr3_1:12294667-12294686 | Msa0349080:intron | 25.0% |
| ATGAGGCTAAGAATATGAAA+GGG | - | chr3_1:12292905-12292924 | None:intergenic | 30.0% | |
| GTAGATGGAGGATATTATTA+TGG | + | chr3_1:12292993-12293012 | Msa0349080:five_prime_UTR | 30.0% | |
| AGAGACTAAAAAGAGATGAA+TGG | + | chr3_1:12293137-12293156 | Msa0349080:CDS | 30.0% | |
| AGAAATAGAGCAAAACTCAA+AGG | + | chr3_1:12293214-12293233 | Msa0349080:CDS | 30.0% | |
| AAAACTCAAAGGACAAGATT+GGG | + | chr3_1:12293225-12293244 | Msa0349080:CDS | 30.0% | |
| ! | TGATCTATACCTTTTCTTCA+TGG | - | chr3_1:12293335-12293354 | None:intergenic | 30.0% |
| ! | AGATCAAGTCTAGAATACAA+AGG | - | chr3_1:12293388-12293407 | None:intergenic | 30.0% |
| !!! | TAATCAAGGTTTGGTTTTGT+TGG | + | chr3_1:12293453-12293472 | Msa0349080:CDS | 30.0% |
| !! | TGGTGTTGATAAATTAGTCA+AGG | + | chr3_1:12293585-12293604 | Msa0349080:CDS | 30.0% |
| GAATGCTGCTGAATAATTAT+TGG | + | chr3_1:12294586-12294605 | Msa0349080:intron | 30.0% | |
| ! | TGGGAGAATGAATATTTTCA+TGG | - | chr3_1:12294723-12294742 | None:intergenic | 30.0% |
| ATGGATTCTTGTTAGCTATA+TGG | - | chr3_1:12294942-12294961 | None:intergenic | 30.0% | |
| TATAGCTAACAAGAATCCAT+TGG | + | chr3_1:12294942-12294961 | Msa0349080:CDS | 30.0% | |
| AGGCAAAAGAAAGAAACAAT+GGG | + | chr3_1:12295057-12295076 | Msa0349080:CDS | 30.0% | |
| ! | GTTGATTCATCATTGAGAAT+TGG | + | chr3_1:12295285-12295304 | Msa0349080:CDS | 30.0% |
| !!! | GCTTGTTTTTTTCTTCTTCT+TGG | + | chr3_1:12295487-12295506 | Msa0349080:three_prime_UTR | 30.0% |
| CATGAGGCTAAGAATATGAA+AGG | - | chr3_1:12292906-12292925 | None:intergenic | 35.0% | |
| !! | AAAGTTTTGTAGCTCACATG+AGG | - | chr3_1:12292922-12292941 | None:intergenic | 35.0% |
| ! | ATTCTTGTTGTGATGTTGGA+GGG | - | chr3_1:12293069-12293088 | None:intergenic | 35.0% |
| ATTATTATTGGCAGTGGAAG+TGG | + | chr3_1:12293109-12293128 | Msa0349080:CDS | 35.0% | |
| AAAAGAGATGAATGGAGTGA+AGG | + | chr3_1:12293145-12293164 | Msa0349080:CDS | 35.0% | |
| CAAAACTCAAAGGACAAGAT+TGG | + | chr3_1:12293224-12293243 | Msa0349080:CDS | 35.0% | |
| TTACACTGTGTTTGTGTCTT+TGG | - | chr3_1:12293298-12293317 | None:intergenic | 35.0% | |
| AGATTGAGTCCATGAAGAAA+AGG | + | chr3_1:12293323-12293342 | Msa0349080:CDS | 35.0% | |
| CTAGTTCAGATGTTTCATCT+TGG | + | chr3_1:12293362-12293381 | Msa0349080:CDS | 35.0% | |
| ! | TTTTGAGCAGTTGTAATTGG+TGG | - | chr3_1:12293550-12293569 | None:intergenic | 35.0% |
| !!! | GAGTTTTGAGCAGTTGTAAT+TGG | - | chr3_1:12293553-12293572 | None:intergenic | 35.0% |
| CAAAACTCACATGGATCAAA+TGG | + | chr3_1:12293565-12293584 | Msa0349080:CDS | 35.0% | |
| TATTCTCACTTGTCTAAGCA+AGG | - | chr3_1:12294336-12294355 | None:intergenic | 35.0% | |
| ! | CAAGTGAGAATATAACTTGC+AGG | + | chr3_1:12294344-12294363 | Msa0349080:intron | 35.0% |
| CAGAGAACAATAACAACAAG+AGG | + | chr3_1:12295037-12295056 | Msa0349080:CDS | 35.0% | |
| GAGGCAAAAGAAAGAAACAA+TGG | + | chr3_1:12295056-12295075 | Msa0349080:CDS | 35.0% | |
| AAGGTCAGAACAAACAAGAA+TGG | + | chr3_1:12295119-12295138 | Msa0349080:CDS | 35.0% | |
| CATCTTCTCTATCTCTTTCA+GGG | - | chr3_1:12295148-12295167 | None:intergenic | 35.0% | |
| TCATCTTCTCTATCTCTTTC+AGG | - | chr3_1:12295149-12295168 | None:intergenic | 35.0% | |
| ATACACAACTAGAGATTGCA+AGG | + | chr3_1:12295229-12295248 | Msa0349080:CDS | 35.0% | |
| TGGAAGAAGCTAATGATATG+TGG | + | chr3_1:12295305-12295324 | Msa0349080:exon | 35.0% | |
| ! | GATTCTTGTTGTGATGTTGG+AGG | - | chr3_1:12293070-12293089 | None:intergenic | 40.0% |
| ! | GGTGATTCTTGTTGTGATGT+TGG | - | chr3_1:12293073-12293092 | None:intergenic | 40.0% |
| AGACTTGATCTTCTTCTTCG+TGG | + | chr3_1:12293397-12293416 | Msa0349080:CDS | 40.0% | |
| GACTTGATCTTCTTCTTCGT+GGG | + | chr3_1:12293398-12293417 | Msa0349080:CDS | 40.0% | |
| TGTGCATGTGACATAACAAG+TGG | - | chr3_1:12293484-12293503 | None:intergenic | 40.0% | |
| ACAACTGCTCAAAACTCACA+TGG | + | chr3_1:12293556-12293575 | Msa0349080:CDS | 40.0% | |
| AGTACCTTAGTGCGTTACTT+AGG | + | chr3_1:12294550-12294569 | Msa0349080:intron | 40.0% | |
| AAGGTACAAAAGGTTTCCCA+TGG | + | chr3_1:12294677-12294696 | Msa0349080:intron | 40.0% | |
| ! | TGGGAATAGCAGAAAGCATA+AGG | + | chr3_1:12295076-12295095 | Msa0349080:CDS | 40.0% |
| ! | GAATAGCAGAAAGCATAAGG+TGG | + | chr3_1:12295079-12295098 | Msa0349080:CDS | 40.0% |
| TGGTCATCTCTTCACTAGTT+AGG | - | chr3_1:12295375-12295394 | None:intergenic | 40.0% | |
| AGAGAGAGCCACTAGCATTA+TGG | - | chr3_1:12292812-12292831 | None:intergenic | 45.0% | |
| GCATGTGACATAACAAGTGG+TGG | - | chr3_1:12293481-12293500 | None:intergenic | 45.0% | |
| TGAGCAGTTGTAATTGGTGG+AGG | - | chr3_1:12293547-12293566 | None:intergenic | 45.0% | |
| GCATCCTAAGTAACGCACTA+AGG | - | chr3_1:12294557-12294576 | None:intergenic | 45.0% | |
| !! | TCTTTTTCGCTGTAAAGCGC+CGG | - | chr3_1:12294985-12295004 | None:intergenic | 45.0% |
| ACGAGCAGAGAAGCAAAAAG+AGG | + | chr3_1:12295004-12295023 | Msa0349080:CDS | 45.0% | |
| GAAGATGAGAGTTGAAGCTG+AGG | + | chr3_1:12295161-12295180 | Msa0349080:CDS | 45.0% | |
| TGAGGCAAAGAGAGGAGAAA+TGG | + | chr3_1:12295179-12295198 | Msa0349080:CDS | 45.0% | |
| !!! | TTTTATATAAAAAAGAAAAA+GGG | - | chr3_1:12292781-12292800 | None:intergenic | 5.0% |
| !!! | TTTTTATATAAAAAAGAAAA+AGG | - | chr3_1:12292782-12292801 | None:intergenic | 5.0% |
| GATCTTCTTCTTCGTGGGAC+AGG | + | chr3_1:12293403-12293422 | Msa0349080:CDS | 50.0% | |
| TCAGCCACTAGCTCCTAGTA+CGG | + | chr3_1:12293516-12293535 | Msa0349080:CDS | 50.0% | |
| ACAACCGTACTAGGAGCTAG+TGG | - | chr3_1:12293523-12293542 | None:intergenic | 50.0% | |
| !! | GTGCTGCTATCGGTTTCCAA+TGG | - | chr3_1:12294961-12294980 | None:intergenic | 50.0% |
| AAGCATAAGGTGGCTAGCAG+AGG | + | chr3_1:12295089-12295108 | Msa0349080:CDS | 50.0% | |
| GTTGAAGCTGAGGCAAAGAG+AGG | + | chr3_1:12295171-12295190 | Msa0349080:CDS | 50.0% | |
| GGTGGAGGAACAACCGTACT+AGG | - | chr3_1:12293532-12293551 | None:intergenic | 55.0% | |
| CATAAGGTGGCTAGCAGAGG+TGG | + | chr3_1:12295092-12295111 | Msa0349080:CDS | 55.0% | |
| GGAAACCGATAGCAGCACGC+CGG | + | chr3_1:12294963-12294982 | Msa0349080:CDS | 60.0% | |
| AAGGTGGCTAGCAGAGGTGG+TGG | + | chr3_1:12295095-12295114 | Msa0349080:CDS | 60.0% | |
| ! | GGCTAGCAGAGGTGGTGGTA+AGG | + | chr3_1:12295100-12295119 | Msa0349080:CDS | 60.0% |
| !! | AAGCGCCGGCGTGCTGCTAT+CGG | - | chr3_1:12294971-12294990 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_1 | gene | 12292748 | 12295556 | 12292748 | ID=Msa0349080;Name=Msa0349080 |
| chr3_1 | mRNA | 12292748 | 12295556 | 12292748 | ID=Msa0349080-mRNA-1;Parent=Msa0349080;Name=Msa0349080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=277|1|1|1|1|1|2|239|329 |
| chr3_1 | exon | 12292748 | 12293606 | 12292748 | ID=Msa0349080-mRNA-1:exon:3212;Parent=Msa0349080-mRNA-1 |
| chr3_1 | exon | 12294910 | 12295556 | 12294910 | ID=Msa0349080-mRNA-1:exon:3213;Parent=Msa0349080-mRNA-1 |
| chr3_1 | five_prime_UTR | 12292748 | 12293024 | 12292748 | ID=Msa0349080-mRNA-1:five_prime_utr;Parent=Msa0349080-mRNA-1 |
| chr3_1 | CDS | 12293025 | 12293606 | 12293025 | ID=Msa0349080-mRNA-1:cds;Parent=Msa0349080-mRNA-1 |
| chr3_1 | CDS | 12294910 | 12295317 | 12294910 | ID=Msa0349080-mRNA-1:cds;Parent=Msa0349080-mRNA-1 |
| chr3_1 | three_prime_UTR | 12295318 | 12295556 | 12295318 | ID=Msa0349080-mRNA-1:three_prime_utr;Parent=Msa0349080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
