AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0835310


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACCAGTTTGCTTTGATTC+AGG 0.268324 5_4:+8612273 None:intergenic
TTAATTACTGTGCATAATTT+AGG 0.285992 5_4:-8610722 Msa0835310:CDS
TTCAGTGCTCCGATTGACTA+TGG 0.337482 5_4:-8612253 Msa0835310:CDS
AGACGGTGTTTACTTCTTCA+TGG 0.386934 5_4:-8610810 Msa0835310:CDS
GTTTCAATACCGAACCGAAC+TGG 0.463267 5_4:-8612231 Msa0835310:intron
TCACCTGAATCAAAGCAAAC+TGG 0.466576 5_4:-8612276 Msa0835310:CDS
ATTGAAAGACATATACGGTA+AGG 0.475732 5_4:-8610605 Msa0835310:three_prime_UTR
TTCGATCATAATGCTGCTGT+TGG 0.516967 5_4:-8610759 Msa0835310:CDS
CACAGTAATTAAATACATGC+AGG 0.536586 5_4:+8610733 None:intergenic
AAATGCTTCATGGATCAATC+CGG 0.543945 5_4:+8610680 None:intergenic
TTGAAAGACATATACGGTAA+GGG 0.547780 5_4:-8610604 Msa0835310:three_prime_UTR
ACATGATTGAAGCATTATGT+TGG 0.555415 5_4:-8610784 Msa0835310:CDS
TGAAAGACATATACGGTAAG+GGG 0.560843 5_4:-8610603 Msa0835310:three_prime_UTR
GTATTGAAACCATAGTCAAT+CGG 0.577926 5_4:+8612244 None:intergenic
TGTAACAATACTACTGTGTG+TGG 0.674736 5_4:-8610578 Msa0835310:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAACAGAATTAAAACA+CGG - chr5_4:8611728-8611747 Msa0835310:intron 15.0%
!!! TTTTTATTGAAAGACATATA+CGG - chr5_4:8612200-8612219 Msa0835310:intron 15.0%
!! ATATATTTGCATTTAGTGTT+TGG + chr5_4:8610643-8610662 None:intergenic 20.0%
!! AAACACTAAATGCAAATATA+TGG - chr5_4:8610642-8610661 Msa0835310:three_prime_UTR 20.0%
!! ATTTAGTGTTTACAACAAAA+TGG + chr5_4:8610718-8610737 None:intergenic 20.0%
!! ATTTAGTGTTTACAACAAAA+TGG + chr5_4:8610718-8610737 None:intergenic 20.0%
!!! CAGTTTTTTTAAGGATAATT+TGG - chr5_4:8611777-8611796 Msa0835310:intron 20.0%
!! ACAAAGTTGCAAAAATAAAA+CGG + chr5_4:8611978-8611997 None:intergenic 20.0%
!! TTAATTACTGTGCATAATTT+AGG - chr5_4:8612088-8612107 Msa0835310:intron 20.0%
!! CAAGATGCAAAATTTTGAAT+CGG + chr5_4:8610612-8610631 None:intergenic 25.0%
!! ACCTAAATAGTCTTAGAATT+TGG - chr5_4:8610839-8610858 Msa0835310:intron 25.0%
!! CCTAAATAGTCTTAGAATTT+GGG - chr5_4:8610840-8610859 Msa0835310:intron 25.0%
!!! AGCTTTAAATGTTATCGAAA+AGG - chr5_4:8611445-8611464 Msa0835310:intron 25.0%
! TTATCGAAAAGGAAATTCAA+TGG - chr5_4:8611456-8611475 Msa0835310:intron 25.0%
!!! TTAACGGTTCAGTTTTTTTA+AGG - chr5_4:8611768-8611787 Msa0835310:intron 25.0%
GTATTGAAACCATAGTCAAT+CGG + chr5_4:8610569-8610588 None:intergenic 30.0%
!! CCCAAATTCTAAGACTATTT+AGG + chr5_4:8610843-8610862 None:intergenic 30.0%
! AATAGTCTTAGAATTTGGGT+TGG - chr5_4:8610844-8610863 Msa0835310:intron 30.0%
! ATAGTCTTAGAATTTGGGTT+GGG - chr5_4:8610845-8610864 Msa0835310:intron 30.0%
CTTATAAACCCATATGTTCA+GGG - chr5_4:8610915-8610934 Msa0835310:intron 30.0%
TTATAAACCCATATGTTCAG+GGG - chr5_4:8610916-8610935 Msa0835310:intron 30.0%
TCACTTGTAAATCCATATTC+AGG - chr5_4:8611070-8611089 Msa0835310:intron 30.0%
CACTTGTAAATCCATATTCA+GGG - chr5_4:8611071-8611090 Msa0835310:intron 30.0%
ACTTGTAAATCCATATTCAG+GGG - chr5_4:8611072-8611091 Msa0835310:intron 30.0%
CTAATTCAACCTTACAAAAC+CGG - chr5_4:8611252-8611271 Msa0835310:intron 30.0%
ATGTTCTTTCTGTGGATTTA+AGG + chr5_4:8611494-8611513 None:intergenic 30.0%
TCACCAAGTGATGATAAATT+AGG - chr5_4:8611513-8611532 Msa0835310:intron 30.0%
CACCAAGTGATGATAAATTA+GGG - chr5_4:8611514-8611533 Msa0835310:intron 30.0%
ACCAAGTGATGATAAATTAG+GGG - chr5_4:8611515-8611534 Msa0835310:intron 30.0%
GGGGGATATAATTGATAAAA+CGG - chr5_4:8611535-8611554 Msa0835310:intron 30.0%
GGGGATATAATTGATAAAAC+GGG - chr5_4:8611536-8611555 Msa0835310:intron 30.0%
GGGATATAATTGATAAAACG+GGG - chr5_4:8611537-8611556 Msa0835310:intron 30.0%
GGATATAATTGATAAAACGG+GGG - chr5_4:8611538-8611557 Msa0835310:intron 30.0%
GATATAATTGATAAAACGGG+GGG - chr5_4:8611539-8611558 Msa0835310:intron 30.0%
ATATAATTGATAAAACGGGG+GGG - chr5_4:8611540-8611559 Msa0835310:intron 30.0%
! TTTCTTACCCATGAAGTTTT+AGG - chr5_4:8611591-8611610 Msa0835310:intron 30.0%
GAATTCACCTAAAACTTCAT+GGG + chr5_4:8611601-8611620 None:intergenic 30.0%
AGAAAACAACTCTTACATTG+TGG - chr5_4:8611629-8611648 Msa0835310:intron 30.0%
GAAAACAACTCTTACATTGT+GGG - chr5_4:8611630-8611649 Msa0835310:intron 30.0%
AACTTCAAATTTCCACAGTT+TGG + chr5_4:8611700-8611719 None:intergenic 30.0%
! AACTGTGGAAATTTGAAGTT+TGG - chr5_4:8611700-8611719 Msa0835310:intron 30.0%
TCAGTTTGCCAATCATTTAA+CGG - chr5_4:8611752-8611771 Msa0835310:intron 30.0%
AAACTGAACCGTTAAATGAT+TGG + chr5_4:8611763-8611782 None:intergenic 30.0%
! AAGGATAATTTGGTTCAGTT+TGG - chr5_4:8611787-8611806 Msa0835310:intron 30.0%
TTCTAATACCTGCATTGAAT+TGG - chr5_4:8611929-8611948 Msa0835310:intron 30.0%
AAGATAAACCAATTCAATGC+AGG + chr5_4:8611940-8611959 None:intergenic 30.0%
! ACATGATTGAAGCATTATGT+TGG - chr5_4:8612026-8612045 Msa0835310:intron 30.0%
CACAGTAATTAAATACATGC+AGG + chr5_4:8612080-8612099 None:intergenic 30.0%
ATTGAAAGACATATACGGTA+AGG - chr5_4:8612205-8612224 Msa0835310:intron 30.0%
TTGAAAGACATATACGGTAA+GGG - chr5_4:8612206-8612225 Msa0835310:intron 30.0%
GAATCGGTAAAAAACCAGTT+CGG + chr5_4:8610596-8610615 None:intergenic 35.0%
!! GACATTGATTTGCATTTCAC+TGG - chr5_4:8610673-8610692 Msa0835310:three_prime_UTR 35.0%
TGTTTACAACAAAATGGAGC+GGG + chr5_4:8610712-8610731 None:intergenic 35.0%
GTGTTTACAACAAAATGGAG+CGG + chr5_4:8610713-8610732 None:intergenic 35.0%
! GAACCTAATTTGCATTTCAC+TGG - chr5_4:8610757-8610776 Msa0835310:CDS 35.0%
GATCCAGTGAAATGCAAATT+AGG + chr5_4:8610763-8610782 None:intergenic 35.0%
TGTTTACAACAAAATGGAGC+GGG + chr5_4:8610712-8610731 None:intergenic 35.0%
GTGTTTACAACAAAATGGAG+CGG + chr5_4:8610713-8610732 None:intergenic 35.0%
GCTTATAAACCCATATGTTC+AGG - chr5_4:8610914-8610933 Msa0835310:intron 35.0%
!! CCTTAAAATCCGGTTTTGTA+AGG + chr5_4:8611264-8611283 None:intergenic 35.0%
! CCTTACAAAACCGGATTTTA+AGG - chr5_4:8611261-8611280 Msa0835310:intron 35.0%
ATGATATAAGGCTTGGACAT+GGG + chr5_4:8611306-8611325 None:intergenic 35.0%
ACATTGGATGATATAAGGCT+TGG + chr5_4:8611313-8611332 None:intergenic 35.0%
! AAGCCTTATATCATCCAATG+TGG - chr5_4:8611312-8611331 Msa0835310:intron 35.0%
! AGCCTTATATCATCCAATGT+GGG - chr5_4:8611313-8611332 Msa0835310:intron 35.0%
CATGAGTTGTGAATTCAGAA+AGG - chr5_4:8611418-8611437 Msa0835310:intron 35.0%
CCAAGTGATGATAAATTAGG+GGG - chr5_4:8611516-8611535 Msa0835310:intron 35.0%
CAAGTGATGATAAATTAGGG+GGG - chr5_4:8611517-8611536 Msa0835310:intron 35.0%
CGAATTCACCTAAAACTTCA+TGG + chr5_4:8611602-8611621 None:intergenic 35.0%
TGGTGAACTGAAACAAATTG+TGG + chr5_4:8611680-8611699 None:intergenic 35.0%
! TTTTTGCAACTTTGTGCAGA+CGG - chr5_4:8611983-8612002 Msa0835310:intron 35.0%
! CTTGTAAATTTTGAACGTGC+CGG - chr5_4:8612111-8612130 Msa0835310:intron 35.0%
AAATGCTTCATGGATCAATC+CGG + chr5_4:8612133-8612152 None:intergenic 35.0%
CTCATGACAAAAATGCTTCA+TGG + chr5_4:8612143-8612162 None:intergenic 35.0%
! AAGCATTTTTGTCATGAGTC+AGG - chr5_4:8612145-8612164 Msa0835310:intron 35.0%
TGAAAGACATATACGGTAAG+GGG - chr5_4:8612207-8612226 Msa0835310:intron 35.0%
TGTAACAATACTACTGTGTG+TGG - chr5_4:8612232-8612251 Msa0835310:CDS 35.0%
TCACCTGAATCAAAGCAAAC+TGG - chr5_4:8610534-8610553 Msa0835310:three_prime_UTR 40.0%
! GAACCAGTTTGCTTTGATTC+AGG + chr5_4:8610540-8610559 None:intergenic 40.0%
GGTAAAAAACCAGTTCGGTT+CGG + chr5_4:8610591-8610610 None:intergenic 40.0%
! GCGAATTTGCAAGGTTGATT+AGG + chr5_4:8610871-8610890 None:intergenic 40.0%
CCTTAAAAGGCGAATTTGCA+AGG + chr5_4:8610880-8610899 None:intergenic 40.0%
! CCTTGCAAATTCGCCTTTTA+AGG - chr5_4:8610877-8610896 Msa0835310:intron 40.0%
GATAAGTCCCCTGAACATAT+GGG + chr5_4:8610926-8610945 None:intergenic 40.0%
AGATAAGTCCCCTGAACATA+TGG + chr5_4:8610927-8610946 None:intergenic 40.0%
TGTGTGTTTGTGTCTCACAT+TGG + chr5_4:8610953-8610972 None:intergenic 40.0%
TACTATAAGGTCACCGCTAT+CGG + chr5_4:8611012-8611031 None:intergenic 40.0%
TGAGATAAGTCCCCTGAATA+TGG + chr5_4:8611085-8611104 None:intergenic 40.0%
GGACTTATCTCATCCAATGT+GGG - chr5_4:8610937-8610956 Msa0835310:intron 40.0%
ATGTTCAGTCTTGACATGAG+AGG + chr5_4:8611132-8611151 None:intergenic 40.0%
TCATGTCAAGACTGAACATC+TGG - chr5_4:8611133-8611152 Msa0835310:intron 40.0%
AAGCCTATACAAGATCTGCT+TGG + chr5_4:8611204-8611223 None:intergenic 40.0%
!!! CAAAACCGGATTTTAAGGTG+AGG - chr5_4:8611266-8611285 Msa0835310:intron 40.0%
GCAAACCTCACCTTAAAATC+CGG + chr5_4:8611274-8611293 None:intergenic 40.0%
GATGATATAAGGCTTGGACA+TGG + chr5_4:8611307-8611326 None:intergenic 40.0%
GTCCCACATTGGATGATATA+AGG + chr5_4:8611318-8611337 None:intergenic 40.0%
TACATGTTAGAGTCCCACAT+TGG + chr5_4:8611329-8611348 None:intergenic 40.0%
AGCAGACACTAGTCACTTAA+GGG + chr5_4:8611371-8611390 None:intergenic 40.0%
AAGCAGACACTAGTCACTTA+AGG + chr5_4:8611372-8611391 None:intergenic 40.0%
!! ACTTGGTGATGTTCTTTCTG+TGG + chr5_4:8611502-8611521 None:intergenic 40.0%
CCCCCTAATTTATCATCACT+TGG + chr5_4:8611519-8611538 None:intergenic 40.0%
TGTTTCAGTTCACCAAACTG+TGG - chr5_4:8611685-8611704 Msa0835310:intron 40.0%
TTTCACAAGAGACCACGTTT+CGG + chr5_4:8611839-8611858 None:intergenic 40.0%
TGCGGTGATTATCGAAAATG+TGG + chr5_4:8611865-8611884 None:intergenic 40.0%
! ACATTTTCGATAATCACCGC+AGG - chr5_4:8611864-8611883 Msa0835310:intron 40.0%
AGACGGTGTTTACTTCTTCA+TGG - chr5_4:8612000-8612019 Msa0835310:intron 40.0%
TTCGATCATAATGCTGCTGT+TGG - chr5_4:8612051-8612070 Msa0835310:intron 40.0%
TTCAGTGCTCCGATTGACTA+TGG - chr5_4:8610557-8610576 Msa0835310:three_prime_UTR 45.0%
GTTTCAATACCGAACCGAAC+TGG - chr5_4:8610579-8610598 Msa0835310:three_prime_UTR 45.0%
!!! CAAATTCGCCTTTTAAGGCG+AGG - chr5_4:8610882-8610901 Msa0835310:intron 45.0%
AGTGCAAACCTCGCCTTAAA+AGG + chr5_4:8610893-8610912 None:intergenic 45.0%
!! ATGTTCACTTTTGGGCGTGA+GGG + chr5_4:8610977-8610996 None:intergenic 45.0%
!! CGCTCCAGATGTTCACTTTT+GGG + chr5_4:8610985-8611004 None:intergenic 45.0%
! ACGCTCCAGATGTTCACTTT+TGG + chr5_4:8610986-8611005 None:intergenic 45.0%
ACTATAAGGTCACCGCTATC+GGG + chr5_4:8611011-8611030 None:intergenic 45.0%
! GATAGCGGTGACCTTATAGT+AGG - chr5_4:8611011-8611030 Msa0835310:intron 45.0%
AGGTCTTGGATAGGATCTGT+GGG - chr5_4:8611043-8611062 Msa0835310:intron 45.0%
GGGACTTATCTCATCCAATG+TGG - chr5_4:8610936-8610955 Msa0835310:intron 45.0%
!! CGCATGTTTTTGTCCCACAT+TGG + chr5_4:8611109-8611128 None:intergenic 45.0%
TCAAGACTGAACATCTGGAG+CGG - chr5_4:8611138-8611157 Msa0835310:intron 45.0%
TGCTATAAGGTCACCGCTAT+CGG + chr5_4:8611176-8611195 None:intergenic 45.0%
TGGCCAAGCAGATCTTGTAT+AGG - chr5_4:8611198-8611217 Msa0835310:intron 45.0%
GCAGACACTAGTCACTTAAG+GGG + chr5_4:8611370-8611389 None:intergenic 45.0%
ACTCTTACATTGTGGGTGTG+TGG - chr5_4:8611637-8611656 Msa0835310:intron 45.0%
TTCGATAATCACCGCAGGTT+TGG - chr5_4:8611869-8611888 Msa0835310:intron 45.0%
! TAATCACCGCAGGTTTGGTT+TGG - chr5_4:8611874-8611893 Msa0835310:intron 45.0%
ACCTTACCAAACCAAACCTG+CGG + chr5_4:8611883-8611902 None:intergenic 45.0%
!! TGTTCACTTTTGGGCGTGAG+GGG + chr5_4:8610976-8610995 None:intergenic 50.0%
!! GATGTTCACTTTTGGGCGTG+AGG + chr5_4:8610978-8610997 None:intergenic 50.0%
CACGCCCAAAAGTGAACATC+TGG - chr5_4:8610978-8610997 Msa0835310:intron 50.0%
AGCGGTGACCTTATAGTAGG+TGG - chr5_4:8611014-8611033 Msa0835310:intron 50.0%
! CTGGTGGACCACCTACTATA+AGG + chr5_4:8611025-8611044 None:intergenic 50.0%
CTTATAGTAGGTGGTCCACC+AGG - chr5_4:8611023-8611042 Msa0835310:intron 50.0%
AGATCCTATCCAAGACCTGG+TGG + chr5_4:8611041-8611060 None:intergenic 50.0%
CACAGATCCTATCCAAGACC+TGG + chr5_4:8611044-8611063 None:intergenic 50.0%
CAGGTCTTGGATAGGATCTG+TGG - chr5_4:8611042-8611061 Msa0835310:intron 50.0%
! CAAGACTGAACATCTGGAGC+GGG - chr5_4:8611139-8611158 Msa0835310:intron 50.0%
GCTATAAGGTCACCGCTATC+GGG + chr5_4:8611175-8611194 None:intergenic 50.0%
AGCGGTGACCTTATAGCAGT+TGG - chr5_4:8611178-8611197 Msa0835310:intron 50.0%
CTGCTTGGCCAACTGCTATA+AGG + chr5_4:8611189-8611208 None:intergenic 50.0%
ACTTAAGGGGACAGTGTTGC+TGG + chr5_4:8611357-8611376 None:intergenic 50.0%
TTGTGGGTGTGTGGAACAAC+AGG - chr5_4:8611646-8611665 Msa0835310:intron 50.0%
TGTGGGTGTGTGGAACAACA+GGG - chr5_4:8611647-8611666 Msa0835310:intron 50.0%
! ATTTTCTGCCAGCCGAAACG+TGG - chr5_4:8611824-8611843 Msa0835310:intron 50.0%
!! ACCGCAGGTTTGGTTTGGTA+AGG - chr5_4:8611879-8611898 Msa0835310:intron 50.0%
TGGTCCACCAGGTCTTGGAT+AGG - chr5_4:8611034-8611053 Msa0835310:intron 55.0%
GGTGTGTGGAACAACAGGGA+GGG - chr5_4:8611651-8611670 Msa0835310:intron 55.0%
GTGTGTGGAACAACAGGGAG+GGG - chr5_4:8611652-8611671 Msa0835310:intron 55.0%
TGTGTGGAACAACAGGGAGG+GGG - chr5_4:8611653-8611672 Msa0835310:intron 55.0%
ACAAGAGACCACGTTTCGGC+TGG + chr5_4:8611835-8611854 None:intergenic 55.0%
! TCTGGAGCGTGACCCGATAG+CGG - chr5_4:8610996-8611015 Msa0835310:intron 60.0%
GTAGGTGGTCCACCAGGTCT+TGG - chr5_4:8611029-8611048 Msa0835310:intron 60.0%
GGGTGTGTGGAACAACAGGG+AGG - chr5_4:8611650-8611669 Msa0835310:intron 60.0%
! CATCTGGAGCGGGACCTGAG+TGG - chr5_4:8611149-8611168 Msa0835310:intron 65.0%
GGACCTGAGTGGCCCGATAG+CGG - chr5_4:8611160-8611179 Msa0835310:intron 65.0%
TCACCGCTATCGGGCCACTC+AGG + chr5_4:8611166-8611185 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 8610528 8612304 8610528 ID=Msa0835310;Name=Msa0835310
chr5_4 mRNA 8610528 8612304 8610528 ID=Msa0835310-mRNA-1;Parent=Msa0835310;Name=Msa0835310-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0.5|0.5|1|1|2|187|62
chr5_4 exon 8610528 8610830 8610528 ID=Msa0835310-mRNA-1:exon:3825;Parent=Msa0835310-mRNA-1
chr5_4 exon 8612232 8612304 8612232 ID=Msa0835310-mRNA-1:exon:3824;Parent=Msa0835310-mRNA-1
chr5_4 CDS 8612232 8612304 8612232 ID=Msa0835310-mRNA-1:cds;Parent=Msa0835310-mRNA-1
chr5_4 CDS 8610715 8610830 8610715 ID=Msa0835310-mRNA-1:cds;Parent=Msa0835310-mRNA-1
chr5_4 three_prime_UTR 8610528 8610714 8610528 ID=Msa0835310-mRNA-1:three_prime_utr;Parent=Msa0835310-mRNA-1
Gene Sequence

>Msa0835310

ATGGCTTCACCTGAATCAAAGCAAACTGGTTCAGTGCTCCGATTGACTATGGTTTCAATACCGAACCGAACTGACGGTGTTTACTTCTTCATGGTTGACATGATTGAAGCATTATGTTGGCTTTCGATCATAATGCTGCTGTTGGCTGCCTGCATGTATTTAATTACTGTGCATAATTTAGGCTTGTAA

Protein sequence

>Msa0835310

MASPESKQTGSVLRLTMVSIPNRTDGVYFFMVDMIEALCWLSIIMLLLAACMYLITVHNLGL*