AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0863450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0863450 MtrunA17_Chr5g0445071 100.000 70 0 0 1 70 1 70 4.12e-46 141
Msa0863450 MtrunA17_Chr3g0100571 81.429 70 13 0 1 70 1 70 3.88e-39 123
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0863450 AT4G26710.2 78.571 70 15 0 1 70 1 70 9.65e-37 117
Msa0863450 AT4G26710.1 78.571 70 15 0 1 70 1 70 9.65e-37 117
Msa0863450 AT5G55290.2 72.857 70 19 0 1 70 1 70 1.56e-34 112
Msa0863450 AT5G55290.3 72.857 70 19 0 1 70 1 70 1.56e-34 112
Msa0863450 AT5G55290.1 72.857 70 19 0 1 70 1 70 1.56e-34 112

Find 39 sgRNAs with CRISPR-Local

Find 175 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGTGGGAGTATATGTTTA+AGG 0.311535 5_4:+63200452 Msa0863450:three_prime_UTR
TGGATAGATGTATTTATTGT+TGG 0.370505 5_4:+63200345 Msa0863450:three_prime_UTR
TTTCAGAAGTTGGTAAATAG+TGG 0.384932 5_4:+63200436 Msa0863450:three_prime_UTR
CTGCTTGAGAGAATATGATT+TGG 0.401354 5_4:+63200298 Msa0863450:three_prime_UTR
GCTTCATTCATTTCAGAAGT+TGG 0.411244 5_4:+63200426 Msa0863450:three_prime_UTR
TTGATGTAAGAACCAAAGTT+AGG 0.415305 5_4:-63198672 None:intergenic
TAGTGGGAGTATATGTTTAA+GGG 0.419491 5_4:+63200453 Msa0863450:three_prime_UTR
CAGGTGGGCGATTGTATATC+TGG 0.425794 5_4:+63200178 Msa0863450:intron
TGAAGCAAATTCTTGTAGAA+AGG 0.427326 5_4:-63197699 None:intergenic
ATGTATTTATTGTTGGATAG+TGG 0.428598 5_4:+63200352 Msa0863450:three_prime_UTR
TTGCAGATTTCACCTAACTT+TGG 0.445042 5_4:+63198660 Msa0863450:intron
GGGAAATAAGTTGATGAAAG+GGG 0.454335 5_4:+63200473 Msa0863450:three_prime_UTR
ATGTTTGACACAAGAACATA+TGG 0.468957 5_4:+63200631 Msa0863450:three_prime_UTR
TATATAAGAAGAAAATAAGT+TGG 0.469808 5_4:+63200513 Msa0863450:three_prime_UTR
AGGGAAATAAGTTGATGAAA+GGG 0.469832 5_4:+63200472 Msa0863450:three_prime_UTR
CTCAAGATGGGAACTATGAG+TGG 0.483301 5_4:-63200212 None:intergenic
TTACATCAACAATATGCTGC+TGG 0.486981 5_4:+63198686 Msa0863450:CDS
ACAAGAATTTGCTTCAACAA+AGG 0.488569 5_4:+63197706 Msa0863450:CDS
AAGCAGATCGAAATCTCAAT+GGG 0.493901 5_4:+63197628 Msa0863450:exon
TAAGCAGATCGAAATCTCAA+TGG 0.496028 5_4:+63197627 Msa0863450:exon
AAGGGAAATAAGTTGATGAA+AGG 0.497120 5_4:+63200471 Msa0863450:three_prime_UTR
TTATTCGCCATCACTCAAGA+TGG 0.522896 5_4:-63200225 None:intergenic
CTCTTCTCATTAGTATCACA+TGG 0.531827 5_4:+63200584 Msa0863450:three_prime_UTR
TATTCTCTCAAGCAGACAAT+CGG 0.535030 5_4:-63200290 None:intergenic
TTCAGAAGTTGGTAAATAGT+GGG 0.546216 5_4:+63200437 Msa0863450:three_prime_UTR
TCGCTAATCTTCTTACTCAT+CGG 0.553999 5_4:+63197664 Msa0863450:CDS
ACACAAGAACATATGGTAAG+TGG 0.557073 5_4:+63200638 Msa0863450:three_prime_UTR
TATTCGCCATCACTCAAGAT+GGG 0.588379 5_4:-63200224 None:intergenic
ATAGTTCCCATCTTGAGTGA+TGG 0.596385 5_4:+63200218 Msa0863450:CDS
GGAAATAAGTTGATGAAAGG+GGG 0.607514 5_4:+63200474 Msa0863450:three_prime_UTR
GAACGAACGAACGAACGTGT+AGG 0.630266 5_4:-63197550 None:intergenic
AAACGCTGCAGCAGCAACGG+GGG 0.630395 5_4:-63200666 None:intergenic
ACAGTTACATTACACGTGGA+AGG 0.637926 5_4:-63197520 None:intergenic
CAAGCATACAGATTAGCAGA+AGG 0.638531 5_4:-63197730 None:intergenic
TCAAGATGGGAACTATGAGT+GGG 0.654151 5_4:-63200211 None:intergenic
AAAACGCTGCAGCAGCAACG+GGG 0.658483 5_4:-63200667 None:intergenic
AGCTACAGTTACATTACACG+TGG 0.669670 5_4:-63197524 None:intergenic
TAAGAAGAAACACAGCACGT+TGG 0.701475 5_4:+63197496 None:intergenic
GATGAGTAAGAAGATTAGCG+AGG 0.729543 5_4:-63197662 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAAGAAATTAAGAA+AGG - chr5_4:63197816-63197835 None:intergenic 10.0%
!!! ATTTAATTTATCTTGAGTTA+TGG + chr5_4:63198110-63198129 Msa0863450:intron 15.0%
!! AAACAATTCATAACAAAATA+AGG - chr5_4:63198544-63198563 None:intergenic 15.0%
!!! TATCTTGTTTTTTTTTTTAG+AGG + chr5_4:63199310-63199329 Msa0863450:intron 15.0%
!! TATATAAGAAGAAAATAAGT+TGG + chr5_4:63200513-63200532 Msa0863450:three_prime_UTR 15.0%
!! AAGAAATTAAGAAAGGTTAA+TGG - chr5_4:63197809-63197828 None:intergenic 20.0%
!!! TAAATTCTATTTTATGTGGA+TGG + chr5_4:63197876-63197895 Msa0863450:intron 20.0%
!! ATGATAAGTATGCTAAAATT+TGG + chr5_4:63198158-63198177 Msa0863450:intron 20.0%
!! AATAAGTTGTTTATTCCAAT+AGG + chr5_4:63198183-63198202 Msa0863450:intron 20.0%
!! ATAAGTTGTTTATTCCAATA+GGG + chr5_4:63198184-63198203 Msa0863450:intron 20.0%
!!! TTTTAACAAACATGAAAAGT+TGG - chr5_4:63198449-63198468 None:intergenic 20.0%
!!! AACTTTTCATGTTTGTTAAA+AGG + chr5_4:63198448-63198467 Msa0863450:intron 20.0%
!! TAAGAATGTTAAATCAAACA+TGG - chr5_4:63198723-63198742 None:intergenic 20.0%
!! TTGATTTAACATTCTTAGTT+TGG + chr5_4:63198726-63198745 Msa0863450:intron 20.0%
!! GACATTAGTAATGATAAAAA+TGG - chr5_4:63198966-63198985 None:intergenic 20.0%
!! CTAAGTAAAAGTAATTGAAT+AGG + chr5_4:63199179-63199198 Msa0863450:intron 20.0%
!! ATGAGAAAATTGTAAAGTTT+AGG + chr5_4:63199495-63199514 Msa0863450:intron 20.0%
!!! TATTTATTTGAGTTCTAGTA+AGG + chr5_4:63199522-63199541 Msa0863450:intron 20.0%
!!! TCATTTTTTTATGTATGAGA+TGG + chr5_4:63200032-63200051 Msa0863450:intron 20.0%
! AACAATACAATGAAGAAGTT+TGG - chr5_4:63197768-63197787 None:intergenic 25.0%
!! GCACTAAATTCTATTTTATG+TGG + chr5_4:63197872-63197891 Msa0863450:intron 25.0%
! TAGATTGGATTATCTCATAA+TGG + chr5_4:63197905-63197924 Msa0863450:intron 25.0%
!! ACATGTTCATAATCTGTTTT+CGG + chr5_4:63198033-63198052 Msa0863450:intron 25.0%
! CTAGAATGTCTAATTAAAAC+AGG + chr5_4:63198074-63198093 Msa0863450:intron 25.0%
!! AATAGGGTCTAAGATAAAAA+GGG + chr5_4:63198200-63198219 Msa0863450:intron 25.0%
!! TCTTTGAGAGATTCAAAATA+AGG + chr5_4:63198323-63198342 Msa0863450:intron 25.0%
!! CTTTGAGAGATTCAAAATAA+GGG + chr5_4:63198324-63198343 Msa0863450:intron 25.0%
! ATTAGAACAAACAACTTCAA+GGG - chr5_4:63199089-63199108 None:intergenic 25.0%
! TATTAGAACAAACAACTTCA+AGG - chr5_4:63199090-63199109 None:intergenic 25.0%
! AAGTAGACAAAAGAATATGT+CGG + chr5_4:63199280-63199299 Msa0863450:intron 25.0%
!!! CTTGTTTTTTTTTTTAGAGG+AGG + chr5_4:63199313-63199332 Msa0863450:intron 25.0%
!!! AAAGCACTTATTGATTCATA+AGG - chr5_4:63199416-63199435 None:intergenic 25.0%
! AATTATACTGGACAAGATTA+TGG - chr5_4:63199855-63199874 None:intergenic 25.0%
! AATCTTGTCCAGTATAATTA+CGG + chr5_4:63199856-63199875 Msa0863450:intron 25.0%
! TGATGTTAAAATGAGTGTTA+TGG - chr5_4:63199963-63199982 None:intergenic 25.0%
! ATAAAACAAATCAAACACCA+AGG - chr5_4:63200158-63200177 None:intergenic 25.0%
!!! TTTGATTTGTTTTATCAGGT+GGG + chr5_4:63200163-63200182 Msa0863450:intron 25.0%
! CCATAGAAAAAATTGTATTC+AGG - chr5_4:63200328-63200347 None:intergenic 25.0%
!!! CCTGAATACAATTTTTTCTA+TGG + chr5_4:63200325-63200344 Msa0863450:three_prime_UTR 25.0%
! TGGATAGATGTATTTATTGT+TGG + chr5_4:63200345-63200364 Msa0863450:three_prime_UTR 25.0%
! ATGTATTTATTGTTGGATAG+TGG + chr5_4:63200352-63200371 Msa0863450:three_prime_UTR 25.0%
TGAAGCAAATTCTTGTAGAA+AGG - chr5_4:63197702-63197721 None:intergenic 30.0%
! ACAAGAATTTGCTTCAACAA+AGG + chr5_4:63197706-63197725 Msa0863450:CDS 30.0%
! CTTTTTATCTTAGACCCTAT+TGG - chr5_4:63198201-63198220 None:intergenic 30.0%
! CAATAGGGTCTAAGATAAAA+AGG + chr5_4:63198199-63198218 Msa0863450:intron 30.0%
! ATTTTTGCAGAGTAAGTTTC+TGG + chr5_4:63198420-63198439 Msa0863450:intron 30.0%
! TTTTTGCAGAGTAAGTTTCT+GGG + chr5_4:63198421-63198440 Msa0863450:intron 30.0%
! AGATTTTATCGACATCTTTC+TGG + chr5_4:63198498-63198517 Msa0863450:intron 30.0%
! GATTTTATCGACATCTTTCT+GGG + chr5_4:63198499-63198518 Msa0863450:intron 30.0%
TTGATGTAAGAACCAAAGTT+AGG - chr5_4:63198675-63198694 None:intergenic 30.0%
TACTGAAAAGTTTCACAACT+AGG + chr5_4:63198778-63198797 Msa0863450:intron 30.0%
AAGTGAATAGATTAAGCTTG+GGG - chr5_4:63199055-63199074 None:intergenic 30.0%
GAAGTGAATAGATTAAGCTT+GGG - chr5_4:63199056-63199075 None:intergenic 30.0%
TGAAGTGAATAGATTAAGCT+TGG - chr5_4:63199057-63199076 None:intergenic 30.0%
! TTTACTTAGTGCTGCTAAAA+AGG - chr5_4:63199168-63199187 None:intergenic 30.0%
GCACAAATAAACAGTGAAAA+TGG + chr5_4:63199201-63199220 Msa0863450:intron 30.0%
AGTAGACAAAAGAATATGTC+GGG + chr5_4:63199281-63199300 Msa0863450:intron 30.0%
GTAGTGTGTGTGTTAATAAA+TGG + chr5_4:63199370-63199389 Msa0863450:intron 30.0%
TAAAATTGAACAAACACACG+TGG + chr5_4:63199554-63199573 Msa0863450:intron 30.0%
! TGAATCAAAGCATTTGGAAA+GGG - chr5_4:63199647-63199666 None:intergenic 30.0%
! TTGAATCAAAGCATTTGGAA+AGG - chr5_4:63199648-63199667 None:intergenic 30.0%
TTTCCAAATGCTTTGATTCA+AGG + chr5_4:63199647-63199666 Msa0863450:intron 30.0%
TTCCAAATGCTTTGATTCAA+GGG + chr5_4:63199648-63199667 Msa0863450:intron 30.0%
ATGGAGAAAATGACTTTAGA+TGG + chr5_4:63199671-63199690 Msa0863450:intron 30.0%
GTTTGTATCATACCATCTAA+AGG - chr5_4:63199897-63199916 None:intergenic 30.0%
ATGATACAAACACATTGATC+TGG + chr5_4:63199906-63199925 Msa0863450:intron 30.0%
TGATACAAACACATTGATCT+GGG + chr5_4:63199907-63199926 Msa0863450:intron 30.0%
!!! GGTGTTTGATTTGTTTTATC+AGG + chr5_4:63200159-63200178 Msa0863450:intron 30.0%
!!! GTTTGATTTGTTTTATCAGG+TGG + chr5_4:63200162-63200181 Msa0863450:intron 30.0%
!! TTTCAGAAGTTGGTAAATAG+TGG + chr5_4:63200436-63200455 Msa0863450:three_prime_UTR 30.0%
!! TTCAGAAGTTGGTAAATAGT+GGG + chr5_4:63200437-63200456 Msa0863450:three_prime_UTR 30.0%
! ATAGTGGGAGTATATGTTTA+AGG + chr5_4:63200452-63200471 Msa0863450:three_prime_UTR 30.0%
! TAGTGGGAGTATATGTTTAA+GGG + chr5_4:63200453-63200472 Msa0863450:three_prime_UTR 30.0%
!! AAGGGAAATAAGTTGATGAA+AGG + chr5_4:63200471-63200490 Msa0863450:three_prime_UTR 30.0%
!! AGGGAAATAAGTTGATGAAA+GGG + chr5_4:63200472-63200491 Msa0863450:three_prime_UTR 30.0%
ATGTTTGACACAAGAACATA+TGG + chr5_4:63200631-63200650 Msa0863450:three_prime_UTR 30.0%
TAAGCAGATCGAAATCTCAA+TGG + chr5_4:63197627-63197646 Msa0863450:exon 35.0%
AAGCAGATCGAAATCTCAAT+GGG + chr5_4:63197628-63197647 Msa0863450:exon 35.0%
TCGCTAATCTTCTTACTCAT+CGG + chr5_4:63197664-63197683 Msa0863450:CDS 35.0%
TGTGGATGGTTAGATTAGAT+TGG + chr5_4:63197890-63197909 Msa0863450:intron 35.0%
!!! TAGTTTTGGCAGTGGAAAAA+TGG - chr5_4:63197965-63197984 None:intergenic 35.0%
! TTTTTCCACTGCCAAAACTA+AGG + chr5_4:63197965-63197984 Msa0863450:intron 35.0%
! TTTTCCACTGCCAAAACTAA+GGG + chr5_4:63197966-63197985 Msa0863450:intron 35.0%
! AATGTTGCTTACAGACTTCA+TGG + chr5_4:63198261-63198280 Msa0863450:intron 35.0%
TGACATAACTGATAGATCGT+GGG + chr5_4:63198358-63198377 Msa0863450:intron 35.0%
AGAGAACAGAACTTAGAGAT+AGG - chr5_4:63198623-63198642 None:intergenic 35.0%
TTGCAGATTTCACCTAACTT+TGG + chr5_4:63198660-63198679 Msa0863450:intron 35.0%
TTACATCAACAATATGCTGC+TGG + chr5_4:63198686-63198705 Msa0863450:CDS 35.0%
ACTCGTACAAGTTACAACAT+TGG + chr5_4:63198907-63198926 Msa0863450:intron 35.0%
CAGACGGCTAATAAAAATGA+TGG - chr5_4:63199464-63199483 None:intergenic 35.0%
TCTGACAGCATATACTCTTT+GGG - chr5_4:63199601-63199620 None:intergenic 35.0%
! ATCCCTTGAATCAAAGCATT+TGG - chr5_4:63199653-63199672 None:intergenic 35.0%
AAATGCTTTGATTCAAGGGA+TGG + chr5_4:63199652-63199671 Msa0863450:intron 35.0%
CTTAGTTGAATCACAGCATT+TGG + chr5_4:63199712-63199731 Msa0863450:intron 35.0%
!! TTTTTCAATTCGTAGCCTCT+TGG + chr5_4:63199765-63199784 Msa0863450:intron 35.0%
! TTGGTGAAAGCACAATGTAA+CGG + chr5_4:63199784-63199803 Msa0863450:intron 35.0%
TTGTCCAGTATAATTACGGT+AGG + chr5_4:63199860-63199879 Msa0863450:intron 35.0%
TGTCCAGTATAATTACGGTA+GGG + chr5_4:63199861-63199880 Msa0863450:intron 35.0%
AATTACGGTAGGGAGAAAAT+TGG + chr5_4:63199871-63199890 Msa0863450:intron 35.0%
AACAGAAGTTTATCGACACA+AGG - chr5_4:63200013-63200032 None:intergenic 35.0%
TATTCTCTCAAGCAGACAAT+CGG - chr5_4:63200293-63200312 None:intergenic 35.0%
CTGCTTGAGAGAATATGATT+TGG + chr5_4:63200298-63200317 Msa0863450:three_prime_UTR 35.0%
GCTTCATTCATTTCAGAAGT+TGG + chr5_4:63200426-63200445 Msa0863450:three_prime_UTR 35.0%
!! GGGAAATAAGTTGATGAAAG+GGG + chr5_4:63200473-63200492 Msa0863450:three_prime_UTR 35.0%
!! GGAAATAAGTTGATGAAAGG+GGG + chr5_4:63200474-63200493 Msa0863450:three_prime_UTR 35.0%
CTCTTCTCATTAGTATCACA+TGG + chr5_4:63200584-63200603 Msa0863450:three_prime_UTR 35.0%
ACACAAGAACATATGGTAAG+TGG + chr5_4:63200638-63200657 Msa0863450:three_prime_UTR 35.0%
ACAGTTACATTACACGTGGA+AGG - chr5_4:63197523-63197542 None:intergenic 40.0%
AGCTACAGTTACATTACACG+TGG - chr5_4:63197527-63197546 None:intergenic 40.0%
GATGAGTAAGAAGATTAGCG+AGG - chr5_4:63197665-63197684 None:intergenic 40.0%
CAAGCATACAGATTAGCAGA+AGG - chr5_4:63197733-63197752 None:intergenic 40.0%
TTTCCACTGCCAAAACTAAG+GGG + chr5_4:63197967-63197986 Msa0863450:intron 40.0%
! CTAAACAGACCCCTTAGTTT+TGG - chr5_4:63197979-63197998 None:intergenic 40.0%
! GGGTCTAAGATAAAAAGGGT+TGG + chr5_4:63198204-63198223 Msa0863450:intron 40.0%
TTGCTTACAGACTTCATGGA+AGG + chr5_4:63198265-63198284 Msa0863450:intron 40.0%
CTGACATAACTGATAGATCG+TGG + chr5_4:63198357-63198376 Msa0863450:intron 40.0%
GATCGTGGGAAAATGTGAAT+TGG + chr5_4:63198372-63198391 Msa0863450:intron 40.0%
AGCTTCTCGTTAGAGTTAGA+GGG - chr5_4:63198599-63198618 None:intergenic 40.0%
AAGCTTCTCGTTAGAGTTAG+AGG - chr5_4:63198600-63198619 None:intergenic 40.0%
TTACTTCCTTGGCAAGGTAT+TGG + chr5_4:63198802-63198821 Msa0863450:intron 40.0%
GAAAAATTCCCTACTTGCTC+AGG - chr5_4:63199247-63199266 None:intergenic 40.0%
! AGTAGGGAATTTTTCCACCA+CGG + chr5_4:63199252-63199271 Msa0863450:intron 40.0%
! TTTTGTCTACTTGTGAACCG+TGG - chr5_4:63199272-63199291 None:intergenic 40.0%
CGGCTAATAAAAATGATGGC+AGG - chr5_4:63199460-63199479 None:intergenic 40.0%
CTCATGTAAAACAGTGCAGA+CGG - chr5_4:63199480-63199499 None:intergenic 40.0%
GTCTGACAGCATATACTCTT+TGG - chr5_4:63199602-63199621 None:intergenic 40.0%
GGAAAGGGATTTGATTGTAC+TGG - chr5_4:63199632-63199651 None:intergenic 40.0%
TACATTGTGCTTTCACCAAG+AGG - chr5_4:63199783-63199802 None:intergenic 40.0%
! TGGTGAAAGCACAATGTAAC+GGG + chr5_4:63199785-63199804 Msa0863450:intron 40.0%
!! TCACTTTTCAAGCATGCACT+TGG + chr5_4:63199814-63199833 Msa0863450:intron 40.0%
TCTCCCTACCGTAATTATAC+TGG - chr5_4:63199867-63199886 None:intergenic 40.0%
!! GGAGAAAATTGGCCTTTAGA+TGG + chr5_4:63199882-63199901 Msa0863450:intron 40.0%
TACAAACACATTGATCTGGG+TGG + chr5_4:63199910-63199929 Msa0863450:intron 40.0%
! TGGTACATTTCCTTTTGTCC+CGG + chr5_4:63200052-63200071 Msa0863450:intron 40.0%
TCACAAGCTAGAACTAGTAC+CGG - chr5_4:63200074-63200093 None:intergenic 40.0%
TGAGGGAGAAGAAAAGAGTA+AGG - chr5_4:63200126-63200145 None:intergenic 40.0%
CACCAAGGTAAGCTTAATGA+GGG - chr5_4:63200143-63200162 None:intergenic 40.0%
ACACCAAGGTAAGCTTAATG+AGG - chr5_4:63200144-63200163 None:intergenic 40.0%
TCAAGATGGGAACTATGAGT+GGG - chr5_4:63200214-63200233 None:intergenic 40.0%
ATAGTTCCCATCTTGAGTGA+TGG + chr5_4:63200218-63200237 Msa0863450:CDS 40.0%
TATTCGCCATCACTCAAGAT+GGG - chr5_4:63200227-63200246 None:intergenic 40.0%
TTATTCGCCATCACTCAAGA+TGG - chr5_4:63200228-63200247 None:intergenic 40.0%
! GTTGATGAAAGGGGGTTATT+TGG + chr5_4:63200482-63200501 Msa0863450:three_prime_UTR 40.0%
TTATCGACATCTTTCTGGGC+TGG + chr5_4:63198503-63198522 Msa0863450:intron 45.0%
CGTTAGAGTTAGAGGGAGAA+CGG - chr5_4:63198592-63198611 None:intergenic 45.0%
CACAACTAGGCTTACTTCCT+TGG + chr5_4:63198791-63198810 Msa0863450:intron 45.0%
AAGTGACCAATACCTTGCCA+AGG - chr5_4:63198811-63198830 None:intergenic 45.0%
!! ATGTGTAGCACTGACACTTC+AGG + chr5_4:63198859-63198878 Msa0863450:intron 45.0%
TCGTGTTTGATGTCCTTGTC+TGG + chr5_4:63199005-63199024 Msa0863450:intron 45.0%
CGTGTTTGATGTCCTTGTCT+GGG + chr5_4:63199006-63199025 Msa0863450:intron 45.0%
! GTGCAGTAGCATCATGGTTT+TGG - chr5_4:63199129-63199148 None:intergenic 45.0%
! TGACTTGTGCAGTAGCATCA+TGG - chr5_4:63199135-63199154 None:intergenic 45.0%
ATAGTCATGCCTGAGCAAGT+AGG + chr5_4:63199235-63199254 Msa0863450:intron 45.0%
TAGTCATGCCTGAGCAAGTA+GGG + chr5_4:63199236-63199255 Msa0863450:intron 45.0%
TTGAACAAACACACGTGGAG+TGG + chr5_4:63199559-63199578 Msa0863450:intron 45.0%
!! AGCATATACTCTTTGGGACG+AGG - chr5_4:63199595-63199614 None:intergenic 45.0%
AAACACATTGATCTGGGTGG+AGG + chr5_4:63199913-63199932 Msa0863450:intron 45.0%
AACACATTGATCTGGGTGGA+GGG + chr5_4:63199914-63199933 Msa0863450:intron 45.0%
CGACACAAGGCAGACATATT+TGG - chr5_4:63200000-63200019 None:intergenic 45.0%
! AGAACTAGTACCGGGACAAA+AGG - chr5_4:63200065-63200084 None:intergenic 45.0%
CACAAGCTAGAACTAGTACC+GGG - chr5_4:63200073-63200092 None:intergenic 45.0%
AAGGTTATGCGCGACAAAAC+AGG - chr5_4:63200107-63200126 None:intergenic 45.0%
CTCCCTCATTAAGCTTACCT+TGG + chr5_4:63200138-63200157 Msa0863450:intron 45.0%
CTCAAGATGGGAACTATGAG+TGG - chr5_4:63200215-63200234 None:intergenic 45.0%
GAACGAACGAACGAACGTGT+AGG - chr5_4:63197553-63197572 None:intergenic 50.0%
!!! AGACCCCTTAGTTTTGGCAG+TGG - chr5_4:63197973-63197992 None:intergenic 50.0%
CTAGGCTTACTTCCTTGGCA+AGG + chr5_4:63198796-63198815 Msa0863450:intron 50.0%
GTGTCAGTGCTACACATGTG+AGG - chr5_4:63198856-63198875 None:intergenic 50.0%
CATTGGCAGTCTGACACATG+TGG + chr5_4:63198924-63198943 Msa0863450:intron 50.0%
! ATGAAGCGCTTACCCAGACA+AGG - chr5_4:63199021-63199040 None:intergenic 50.0%
TTGTCTGGGTAAGCGCTTCA+TGG + chr5_4:63199020-63199039 Msa0863450:intron 50.0%
TGTCTACTTGTGAACCGTGG+TGG - chr5_4:63199269-63199288 None:intergenic 50.0%
ACACATTGATCTGGGTGGAG+GGG + chr5_4:63199915-63199934 Msa0863450:intron 50.0%
CAGGTGGGCGATTGTATATC+TGG + chr5_4:63200178-63200197 Msa0863450:intron 50.0%
GGGAGAACGGAGACACAACA+AGG - chr5_4:63198579-63198598 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 63197510 63200677 63197510 ID=Msa0863450;Name=Msa0863450
chr5_4 mRNA 63197510 63200677 63197510 ID=Msa0863450-mRNA-1;Parent=Msa0863450;Name=Msa0863450-mRNA-1;_AED=0.03;_eAED=0.03;_QI=136|1|1|1|0|0.33|3|430|70
chr5_4 exon 63197510 63197743 63197510 ID=Msa0863450-mRNA-1:exon:19893;Parent=Msa0863450-mRNA-1
chr5_4 exon 63198666 63198713 63198666 ID=Msa0863450-mRNA-1:exon:19894;Parent=Msa0863450-mRNA-1
chr5_4 exon 63200181 63200677 63200181 ID=Msa0863450-mRNA-1:exon:19895;Parent=Msa0863450-mRNA-1
chr5_4 five_prime_UTR 63197510 63197645 63197510 ID=Msa0863450-mRNA-1:five_prime_utr;Parent=Msa0863450-mRNA-1
chr5_4 CDS 63197646 63197743 63197646 ID=Msa0863450-mRNA-1:cds;Parent=Msa0863450-mRNA-1
chr5_4 CDS 63198666 63198713 63198666 ID=Msa0863450-mRNA-1:cds;Parent=Msa0863450-mRNA-1
chr5_4 CDS 63200181 63200247 63200181 ID=Msa0863450-mRNA-1:cds;Parent=Msa0863450-mRNA-1
chr5_4 three_prime_UTR 63200248 63200677 63200248 ID=Msa0863450-mRNA-1:three_prime_utr;Parent=Msa0863450-mRNA-1
Gene Sequence

>Msa0863450

ATGGGTTTCGTTGTTACCTCGCTAATCTTCTTACTCATCGGAATCATCGCATGCCTTTCTACAAGAATTTGCTTCAACAAAGGACCTTCTGCTAATCTATTTCACCTAACTTTGGTTCTTACATCAACAATATGCTGCTGGATGATGTGGGCGATTGTATATCTGGCACAGATGAACCCACTCATAGTTCCCATCTTGAGTGATGGCGAATAA
Protein sequence

>Msa0863450

MGFVVTSLIFLLIGIIACLSTRICFNKGPSANLFHLTLVLTSTICCWMMWAIVYLAQMNPLIVPILSDGE*