AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0690250


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0690250 MtrunA17_Chr4g0038821 97.059 68 2 0 1 68 1 68 1.37e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0690250 AT3G22210.1 62.500 64 23 1 5 68 6 68 1.55e-20 77.0

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTCGCTCTTCCAGTTT+TGG 0.231911 4_4:+57917228 Msa0690250:CDS
GTCTTCTAGCTCGCCTCTCT+CGG 0.346161 4_4:-57917458 None:intergenic
GACAGAAATCACTTCAAGAT+TGG 0.349085 4_4:+57917388 Msa0690250:intron
GCTGCAACAATGCCCAAAAC+TGG 0.365881 4_4:-57917241 None:intergenic
AGCTCGCCTCTCTCGGGAGC+TGG 0.374844 4_4:-57917451 None:intergenic
CTCTCTCGGGAGCTGGATGT+TGG 0.452110 4_4:-57917444 None:intergenic
ACAGAAATCACTTCAAGATT+GGG 0.482353 4_4:+57917389 Msa0690250:intron
ATTGAAACTCACTGCATAGA+AGG 0.485260 4_4:-57917275 None:intergenic
TCTTCTAGCTCGCCTCTCTC+GGG 0.500761 4_4:-57917457 None:intergenic
GATGATTCTGAATCAGAGAA+TGG 0.538128 4_4:+57917411 Msa0690250:CDS
CAACATCCAGCTCCCGAGAG+AGG 0.612999 4_4:+57917445 Msa0690250:CDS
GAGTTTCAATGAGATTAGAG+AGG 0.641117 4_4:+57917288 Msa0690250:CDS
GATTCTGAATCAGAGAATGG+AGG 0.645594 4_4:+57917414 Msa0690250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAAATCACTTCAAGATT+GGG + chr4_4:57917389-57917408 Msa0690250:intron 30.0%
ATTGAAACTCACTGCATAGA+AGG - chr4_4:57917278-57917297 None:intergenic 35.0%
GAGTTTCAATGAGATTAGAG+AGG + chr4_4:57917288-57917307 Msa0690250:CDS 35.0%
GACAGAAATCACTTCAAGAT+TGG + chr4_4:57917388-57917407 Msa0690250:intron 35.0%
GATGATTCTGAATCAGAGAA+TGG + chr4_4:57917411-57917430 Msa0690250:CDS 35.0%
! CAATTCGCTCTTCCAGTTTT+GGG + chr4_4:57917229-57917248 Msa0690250:CDS 40.0%
GATTCTGAATCAGAGAATGG+AGG + chr4_4:57917414-57917433 Msa0690250:CDS 40.0%
! GCAATTCGCTCTTCCAGTTT+TGG + chr4_4:57917228-57917247 Msa0690250:CDS 45.0%
GCTGCAACAATGCCCAAAAC+TGG - chr4_4:57917244-57917263 None:intergenic 50.0%
!! TCTTCTAGCTCGCCTCTCTC+GGG - chr4_4:57917460-57917479 None:intergenic 55.0%
!! GTCTTCTAGCTCGCCTCTCT+CGG - chr4_4:57917461-57917480 None:intergenic 55.0%
CTCTCTCGGGAGCTGGATGT+TGG - chr4_4:57917447-57917466 None:intergenic 60.0%
CAACATCCAGCTCCCGAGAG+AGG + chr4_4:57917445-57917464 Msa0690250:CDS 60.0%
AGCTCGCCTCTCTCGGGAGC+TGG - chr4_4:57917454-57917473 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 57917217 57917506 57917217 ID=Msa0690250;Name=Msa0690250
chr4_4 mRNA 57917217 57917506 57917217 ID=Msa0690250-mRNA-1;Parent=Msa0690250;Name=Msa0690250-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|68
chr4_4 exon 57917217 57917309 57917217 ID=Msa0690250-mRNA-1:exon:15481;Parent=Msa0690250-mRNA-1
chr4_4 exon 57917393 57917506 57917393 ID=Msa0690250-mRNA-1:exon:15482;Parent=Msa0690250-mRNA-1
chr4_4 CDS 57917217 57917309 57917217 ID=Msa0690250-mRNA-1:cds;Parent=Msa0690250-mRNA-1
chr4_4 CDS 57917393 57917506 57917393 ID=Msa0690250-mRNA-1:cds;Parent=Msa0690250-mRNA-1
Gene Sequence

>Msa0690250

ATGGATGCAATGCAATTCGCTCTTCCAGTTTTGGGCATTGTTGCAGCTGCAGCTGTGACCTTCTATGCAGTGAGTTTCAATGAGATTAGAGAGAAATCACTTCAAGATTGGGATGATTCTGAATCAGAGAATGGAGGTTTTAGACCAACATCCAGCTCCCGAGAGAGGCGAGCTAGAAGACAAGCTAGTAAAAATACCAAAAAATAA

Protein sequence

>Msa0690250

MDAMQFALPVLGIVAAAAVTFYAVSFNEIREKSLQDWDDSESENGGFRPTSSSRERRARRQASKNTKK*