AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1453530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453530 MtrunA17_Chr4g0063571 100.000 128 0 0 1 128 1 128 1.30e-91 260
Msa1453530 MtrunA17_Chr2g0307081 97.656 128 3 0 1 128 1 128 3.04e-89 254
Msa1453530 MtrunA17_Chr4g0061981 34.483 116 75 1 4 118 17 132 1.14e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1453530 AT4G22380.1 93.750 128 8 0 1 128 1 128 1.17e-76 223
Msa1453530 AT5G20160.1 92.969 128 9 0 1 128 1 128 4.97e-76 221
Msa1453530 AT4G12600.1 89.844 128 13 0 1 128 1 128 1.31e-72 213
Msa1453530 AT5G20160.2 74.375 160 9 1 1 128 1 160 3.95e-69 205
Msa1453530 AT4G12600.2 55.738 183 26 2 1 128 1 183 2.20e-49 156
Msa1453530 AT5G20160.3 65.741 108 5 1 1 76 1 108 1.15e-31 108
Msa1453530 AT5G08180.2 35.938 128 73 1 8 126 24 151 3.79e-21 83.6
Msa1453530 AT5G08180.1 35.938 128 73 1 8 126 24 151 3.79e-21 83.6

Find 50 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAAAGCGGCTAGGGTTTA+TGG 0.198145 tig0034754:+19651 None:intergenic
TAACTATAAGCAACTCAAAA+AGG 0.236397 tig0034754:-19398 Msa1453530:CDS
GAAGACTTTATATATATAAT+TGG 0.242176 tig0034754:+19708 None:intergenic
TATTTCTGAATTTGTTGTTA+TGG 0.302213 tig0034754:-19253 Msa1453530:CDS
TTGTTGAACAAGATCCATTA+TGG 0.342840 tig0034754:+19426 None:intergenic
TCCGTTACTTGCTGAGGATA+AGG 0.383122 tig0034754:-19193 Msa1453530:intron
AGCTACTAAGACCTTGAATA+GGG 0.384258 tig0034754:-19277 Msa1453530:CDS
TCAGCCAAAGGGTATGCCTT+AGG 0.396497 tig0034754:+19466 None:intergenic
AGTTGTGCTGAAAGCGTCTT+CGG 0.405427 tig0034754:-18142 Msa1453530:three_prime_UTR
GCGTCTTCGGAGGCCTTGAC+TGG 0.409377 tig0034754:-18129 Msa1453530:three_prime_UTR
CAGCTACTAAGACCTTGAAT+AGG 0.416543 tig0034754:-19278 Msa1453530:CDS
TTCAGAAATACCCCTATTCA+AGG 0.432400 tig0034754:+19266 None:intergenic
GTCTTCGTTTCTTATAATAA+CGG 0.434891 tig0034754:-19691 Msa1453530:five_prime_UTR
AACGGTACCATTGTTAATTG+AGG 0.440521 tig0034754:+19614 None:intergenic
TAACCCTAAGGCATACCCTT+TGG 0.467615 tig0034754:-19470 Msa1453530:CDS
TACCACACAATAACAACCTT+CGG 0.470397 tig0034754:+18048 None:intergenic
TCAGCTAACAATCACCATAA+TGG 0.473714 tig0034754:-19440 Msa1453530:CDS
CAGCCAAAGGGTATGCCTTA+GGG 0.474338 tig0034754:+19467 None:intergenic
CCTCAGCAAGTAACGGAAGA+TGG 0.475355 tig0034754:+19199 None:intergenic
TGTGTTCGTCACATCAAAAC+AGG 0.479560 tig0034754:-18435 Msa1453530:CDS
CCATCTTCCGTTACTTGCTG+AGG 0.512018 tig0034754:-19199 Msa1453530:CDS
CGCCGAAGGTTGTTATTGTG+TGG 0.518320 tig0034754:-18050 Msa1453530:three_prime_UTR
GTCACATCAAAACAGGCACT+TGG 0.523031 tig0034754:-18428 Msa1453530:CDS
TTATCCGTATGATGGTATGA+TGG 0.527837 tig0034754:-18173 Msa1453530:three_prime_UTR
AAACTTCATTATCCGTATGA+TGG 0.529098 tig0034754:-18181 Msa1453530:three_prime_UTR
AGAACAAGCAATCACTGGTC+GGG 0.539786 tig0034754:+18388 None:intergenic
GTAACACTCATGCATCATAC+AGG 0.540573 tig0034754:+17863 None:intergenic
TATCCGTATGATGGTATGAT+GGG 0.540926 tig0034754:-18172 Msa1453530:three_prime_UTR
GAAGAGAATGAAAGCGGCTA+GGG 0.556939 tig0034754:+19644 None:intergenic
GTCACAGAACAAGCAATCAC+TGG 0.562045 tig0034754:+18383 None:intergenic
AAACTGAATTTACTAGTCAA+AGG 0.565236 tig0034754:+17961 None:intergenic
CGAAGAGAATGAAAGCGGCT+AGG 0.565826 tig0034754:+19643 None:intergenic
TTTGATGTGACGAACACATA+GGG 0.566367 tig0034754:+18440 None:intergenic
CAGGCACTTGGGCGTGCTTG+TGG 0.575874 tig0034754:-18416 Msa1453530:CDS
CACAACAAGTCGTCCAGTCA+AGG 0.578850 tig0034754:+18116 None:intergenic
TTAGCTGAGCATCAGCCAAA+GGG 0.590874 tig0034754:+19455 None:intergenic
ACCTTATCCTCAGCAAGTAA+CGG 0.593594 tig0034754:+19192 None:intergenic
AAGCCCATCATACCATCATA+CGG 0.597239 tig0034754:+18169 None:intergenic
GTTAGCTGAGCATCAGCCAA+AGG 0.598758 tig0034754:+19454 None:intergenic
GTCTCAGATTCAGCAACTCA+AGG 0.599622 tig0034754:-18336 Msa1453530:intron
GTTCAATCCTCAATTAACAA+TGG 0.604789 tig0034754:-19621 Msa1453530:intron
TGTTCTGTGACAACTAATGA+AGG 0.613805 tig0034754:-18371 Msa1453530:CDS
CAGAACAAGCAATCACTGGT+CGG 0.618383 tig0034754:+18387 None:intergenic
TGGAGAAGCAGTTAACCCTA+AGG 0.621032 tig0034754:-19482 Msa1453530:CDS
TCACATCAAAACAGGCACTT+GGG 0.623134 tig0034754:-18427 Msa1453530:CDS
TGTGCTGAAAGCGTCTTCGG+AGG 0.641398 tig0034754:-18139 Msa1453530:three_prime_UTR
AGATGGAGAAGAATCTCAAG+AGG 0.644746 tig0034754:+19216 None:intergenic
GCTACTAAGACCTTGAATAG+GGG 0.659558 tig0034754:-19276 Msa1453530:CDS
TTGAACGAAGAGAATGAAAG+CGG 0.665410 tig0034754:+19638 None:intergenic
CACACAATAACAACCTTCGG+CGG 0.714102 tig0034754:+18051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGAATAAGAAATTA+GGG + tig0034754:18183-18202 None:intergenic 10.0%
!! AATTTACACAATTTATAATA+AGG - tig0034754:18650-18669 Msa1453530:intron 10.0%
!! AATATTGAAATAAAATGAAT+CGG + tig0034754:19004-19023 None:intergenic 10.0%
!!! ATTTTATTTCAATATTTTTC+TGG - tig0034754:19008-19027 Msa1453530:intron 10.0%
!! AAAAAATGAATAAGAAATTA+GGG + tig0034754:18183-18202 None:intergenic 10.0%
!! AATTTACACAATTTATAATA+AGG - tig0034754:18650-18669 Msa1453530:intron 10.0%
!! AATATTGAAATAAAATGAAT+CGG + tig0034754:19004-19023 None:intergenic 10.0%
!!! ATTTTATTTCAATATTTTTC+TGG - tig0034754:19008-19027 Msa1453530:intron 10.0%
!! GAAGACTTTATATATATAAT+TGG + tig0034754:17834-17853 None:intergenic 15.0%
!! ATTAACAAGAAGATATAAAA+CGG + tig0034754:17946-17965 None:intergenic 15.0%
!! CAAAAAATGAATAAGAAATT+AGG + tig0034754:18184-18203 None:intergenic 15.0%
!! ATTAATCAATCAATAATGAA+AGG + tig0034754:18224-18243 None:intergenic 15.0%
!! GGTAAAAAGAAAATTAAAAT+AGG + tig0034754:19470-19489 None:intergenic 15.0%
!!! TTTTTTTTTCCTTCATAAAA+AGG + tig0034754:19545-19564 None:intergenic 15.0%
!!! GTTTGATTTTTTATTTGAAA+TGG - tig0034754:19707-19726 Msa1453530:five_prime_UTR 15.0%
!! CTTTATATATATAATTGGTT+TGG + tig0034754:17829-17848 None:intergenic 15.0%
!! GAAGACTTTATATATATAAT+TGG + tig0034754:17834-17853 None:intergenic 15.0%
!! ATTAACAAGAAGATATAAAA+CGG + tig0034754:17946-17965 None:intergenic 15.0%
!! CAAAAAATGAATAAGAAATT+AGG + tig0034754:18184-18203 None:intergenic 15.0%
!! ATTAATCAATCAATAATGAA+AGG + tig0034754:18224-18243 None:intergenic 15.0%
!! GGTAAAAAGAAAATTAAAAT+AGG + tig0034754:19470-19489 None:intergenic 15.0%
!!! TTTTTTTTTCCTTCATAAAA+AGG + tig0034754:19545-19564 None:intergenic 15.0%
!!! GTTTGATTTTTTATTTGAAA+TGG - tig0034754:19707-19726 Msa1453530:five_prime_UTR 15.0%
!! CAAATTAAGAGTTAAACATA+GGG + tig0034754:17973-17992 None:intergenic 20.0%
!! TCAAATTAAGAGTTAAACAT+AGG + tig0034754:17974-17993 None:intergenic 20.0%
!!! TATTTCTGAATTTGTTGTTA+TGG - tig0034754:18286-18305 Msa1453530:intron 20.0%
!!! ATTTTAGTTTTTTCATGACT+TGG - tig0034754:18467-18486 Msa1453530:intron 20.0%
!! GTGTAAATTAGTATTAGTAT+AGG + tig0034754:18639-18658 None:intergenic 20.0%
!! GAAAATGAATTCTGAATATT+TGG - tig0034754:19297-19316 Msa1453530:intron 20.0%
!! CAAATTAAGAGTTAAACATA+GGG + tig0034754:17973-17992 None:intergenic 20.0%
!! TCAAATTAAGAGTTAAACAT+AGG + tig0034754:17974-17993 None:intergenic 20.0%
!!! TATTTCTGAATTTGTTGTTA+TGG - tig0034754:18286-18305 Msa1453530:intron 20.0%
!!! ATTTTAGTTTTTTCATGACT+TGG - tig0034754:18467-18486 Msa1453530:intron 20.0%
!! GTGTAAATTAGTATTAGTAT+AGG + tig0034754:18639-18658 None:intergenic 20.0%
!! GAAAATGAATTCTGAATATT+TGG - tig0034754:19297-19316 Msa1453530:intron 20.0%
! GTCTTCGTTTCTTATAATAA+CGG - tig0034754:17848-17867 Msa1453530:three_prime_UTR 25.0%
! TAACTATAAGCAACTCAAAA+AGG - tig0034754:18141-18160 Msa1453530:three_prime_UTR 25.0%
! AACTATAAGCAACTCAAAAA+GGG - tig0034754:18142-18161 Msa1453530:three_prime_UTR 25.0%
! AGTAATATTACTTGAAGACA+TGG + tig0034754:18882-18901 None:intergenic 25.0%
! ATAAAATGAATCGGATGAAA+TGG + tig0034754:18995-19014 None:intergenic 25.0%
! ATGAATTCTGAATATTTGGT+TGG - tig0034754:19301-19320 Msa1453530:intron 25.0%
!!! TGTTTTGAACCTTTTTATGA+AGG - tig0034754:19533-19552 Msa1453530:intron 25.0%
! AAACTGAATTTACTAGTCAA+AGG + tig0034754:19581-19600 None:intergenic 25.0%
! GTCTTCGTTTCTTATAATAA+CGG - tig0034754:17848-17867 Msa1453530:three_prime_UTR 25.0%
! TAACTATAAGCAACTCAAAA+AGG - tig0034754:18141-18160 Msa1453530:three_prime_UTR 25.0%
! AACTATAAGCAACTCAAAAA+GGG - tig0034754:18142-18161 Msa1453530:three_prime_UTR 25.0%
! AGTAATATTACTTGAAGACA+TGG + tig0034754:18882-18901 None:intergenic 25.0%
! ATAAAATGAATCGGATGAAA+TGG + tig0034754:18995-19014 None:intergenic 25.0%
! ATGAATTCTGAATATTTGGT+TGG - tig0034754:19301-19320 Msa1453530:intron 25.0%
!!! TGTTTTGAACCTTTTTATGA+AGG - tig0034754:19533-19552 Msa1453530:intron 25.0%
! AAACTGAATTTACTAGTCAA+AGG + tig0034754:19581-19600 None:intergenic 25.0%
GTTCAATCCTCAATTAACAA+TGG - tig0034754:17918-17937 Msa1453530:three_prime_UTR 30.0%
ATGATGAAAAATTTGCAGAC+TGG - tig0034754:18037-18056 Msa1453530:three_prime_UTR 30.0%
TTGTTGAACAAGATCCATTA+TGG + tig0034754:18116-18135 None:intergenic 30.0%
ATGCTATGCATAACAAATAG+CGG + tig0034754:18567-18586 None:intergenic 30.0%
TGTTATGCATAGCATCTTAT+TGG - tig0034754:18572-18591 Msa1453530:intron 30.0%
TTTGTATCCATACAGATGTT+TGG - tig0034754:18603-18622 Msa1453530:intron 30.0%
TTGTATCCATACAGATGTTT+GGG - tig0034754:18604-18623 Msa1453530:intron 30.0%
!!! TGACTTTTAAAACAGGGTTA+TGG - tig0034754:18699-18718 Msa1453530:intron 30.0%
AAACTTCATTATCCGTATGA+TGG - tig0034754:19358-19377 Msa1453530:intron 30.0%
GTTCAATCCTCAATTAACAA+TGG - tig0034754:17918-17937 Msa1453530:three_prime_UTR 30.0%
ATGATGAAAAATTTGCAGAC+TGG - tig0034754:18037-18056 Msa1453530:three_prime_UTR 30.0%
TTGTTGAACAAGATCCATTA+TGG + tig0034754:18116-18135 None:intergenic 30.0%
ATGCTATGCATAACAAATAG+CGG + tig0034754:18567-18586 None:intergenic 30.0%
TGTTATGCATAGCATCTTAT+TGG - tig0034754:18572-18591 Msa1453530:intron 30.0%
TTTGTATCCATACAGATGTT+TGG - tig0034754:18603-18622 Msa1453530:intron 30.0%
TTGTATCCATACAGATGTTT+GGG - tig0034754:18604-18623 Msa1453530:intron 30.0%
!!! TGACTTTTAAAACAGGGTTA+TGG - tig0034754:18699-18718 Msa1453530:intron 30.0%
AAACTTCATTATCCGTATGA+TGG - tig0034754:19358-19377 Msa1453530:intron 30.0%
TTGAACGAAGAGAATGAAAG+CGG + tig0034754:17904-17923 None:intergenic 35.0%
AACGGTACCATTGTTAATTG+AGG + tig0034754:17928-17947 None:intergenic 35.0%
TCAGCTAACAATCACCATAA+TGG - tig0034754:18099-18118 Msa1453530:three_prime_UTR 35.0%
AGCTACTAAGACCTTGAATA+GGG - tig0034754:18262-18281 Msa1453530:intron 35.0%
TTCAGAAATACCCCTATTCA+AGG + tig0034754:18276-18295 None:intergenic 35.0%
TGCTGAGGATAAGGTATATT+AGG - tig0034754:18355-18374 Msa1453530:CDS 35.0%
GCTGAGGATAAGGTATATTA+GGG - tig0034754:18356-18375 Msa1453530:CDS 35.0%
TGCTATGCATAACAAATAGC+GGG + tig0034754:18566-18585 None:intergenic 35.0%
!!! TGATCGCTGACTTTTAAAAC+AGG - tig0034754:18692-18711 Msa1453530:intron 35.0%
!!! GATCGCTGACTTTTAAAACA+GGG - tig0034754:18693-18712 Msa1453530:intron 35.0%
CTTGACATTTCAAAGCAACT+AGG + tig0034754:18775-18794 None:intergenic 35.0%
!! CTAGTTGCTTTGAAATGTCA+AGG - tig0034754:18773-18792 Msa1453530:intron 35.0%
TCTTTCCTCTGAATGTTGTT+AGG - tig0034754:18806-18825 Msa1453530:intron 35.0%
CATGGTTTATCACACTTGTT+TGG + tig0034754:18864-18883 None:intergenic 35.0%
TTTGATGTGACGAACACATA+GGG + tig0034754:19102-19121 None:intergenic 35.0%
! TTTTGATGTGACGAACACAT+AGG + tig0034754:19103-19122 None:intergenic 35.0%
TGTTCTGTGACAACTAATGA+AGG - tig0034754:19168-19187 Msa1453530:intron 35.0%
! TGAATATTTGGTTGGTATGC+AGG - tig0034754:19309-19328 Msa1453530:intron 35.0%
TTATCCGTATGATGGTATGA+TGG - tig0034754:19366-19385 Msa1453530:intron 35.0%
TATCCGTATGATGGTATGAT+GGG - tig0034754:19367-19386 Msa1453530:intron 35.0%
! AATTTTCTTTTTACCGCCGA+AGG - tig0034754:19475-19494 Msa1453530:CDS 35.0%
TACCACACAATAACAACCTT+CGG + tig0034754:19494-19513 None:intergenic 35.0%
TTGAACGAAGAGAATGAAAG+CGG + tig0034754:17904-17923 None:intergenic 35.0%
AACGGTACCATTGTTAATTG+AGG + tig0034754:17928-17947 None:intergenic 35.0%
TCAGCTAACAATCACCATAA+TGG - tig0034754:18099-18118 Msa1453530:three_prime_UTR 35.0%
AGCTACTAAGACCTTGAATA+GGG - tig0034754:18262-18281 Msa1453530:intron 35.0%
TTCAGAAATACCCCTATTCA+AGG + tig0034754:18276-18295 None:intergenic 35.0%
TGCTGAGGATAAGGTATATT+AGG - tig0034754:18355-18374 Msa1453530:CDS 35.0%
GCTGAGGATAAGGTATATTA+GGG - tig0034754:18356-18375 Msa1453530:CDS 35.0%
TGCTATGCATAACAAATAGC+GGG + tig0034754:18566-18585 None:intergenic 35.0%
!!! TGATCGCTGACTTTTAAAAC+AGG - tig0034754:18692-18711 Msa1453530:intron 35.0%
!!! GATCGCTGACTTTTAAAACA+GGG - tig0034754:18693-18712 Msa1453530:intron 35.0%
CTTGACATTTCAAAGCAACT+AGG + tig0034754:18775-18794 None:intergenic 35.0%
!! CTAGTTGCTTTGAAATGTCA+AGG - tig0034754:18773-18792 Msa1453530:intron 35.0%
TCTTTCCTCTGAATGTTGTT+AGG - tig0034754:18806-18825 Msa1453530:intron 35.0%
CATGGTTTATCACACTTGTT+TGG + tig0034754:18864-18883 None:intergenic 35.0%
TTTGATGTGACGAACACATA+GGG + tig0034754:19102-19121 None:intergenic 35.0%
! TTTTGATGTGACGAACACAT+AGG + tig0034754:19103-19122 None:intergenic 35.0%
TGTTCTGTGACAACTAATGA+AGG - tig0034754:19168-19187 Msa1453530:intron 35.0%
! TGAATATTTGGTTGGTATGC+AGG - tig0034754:19309-19328 Msa1453530:intron 35.0%
TTATCCGTATGATGGTATGA+TGG - tig0034754:19366-19385 Msa1453530:intron 35.0%
TATCCGTATGATGGTATGAT+GGG - tig0034754:19367-19386 Msa1453530:intron 35.0%
! AATTTTCTTTTTACCGCCGA+AGG - tig0034754:19475-19494 Msa1453530:CDS 35.0%
TACCACACAATAACAACCTT+CGG + tig0034754:19494-19513 None:intergenic 35.0%
!! CTCAAAAAGGGTGCTAATGA+AGG - tig0034754:18154-18173 Msa1453530:three_prime_UTR 40.0%
CAGCTACTAAGACCTTGAAT+AGG - tig0034754:18261-18280 Msa1453530:intron 40.0%
GCTACTAAGACCTTGAATAG+GGG - tig0034754:18263-18282 Msa1453530:intron 40.0%
AGATGGAGAAGAATCTCAAG+AGG + tig0034754:18326-18345 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+CGG + tig0034754:18350-18369 None:intergenic 40.0%
GCTATGCATAACAAATAGCG+GGG + tig0034754:18565-18584 None:intergenic 40.0%
TGTGTTCGTCACATCAAAAC+AGG - tig0034754:19104-19123 Msa1453530:intron 40.0%
TCACATCAAAACAGGCACTT+GGG - tig0034754:19112-19131 Msa1453530:intron 40.0%
AAGCCCATCATACCATCATA+CGG + tig0034754:19373-19392 None:intergenic 40.0%
GTAACACTCATGCATCATAC+AGG + tig0034754:19679-19698 None:intergenic 40.0%
!! CTCAAAAAGGGTGCTAATGA+AGG - tig0034754:18154-18173 Msa1453530:three_prime_UTR 40.0%
CAGCTACTAAGACCTTGAAT+AGG - tig0034754:18261-18280 Msa1453530:intron 40.0%
GCTACTAAGACCTTGAATAG+GGG - tig0034754:18263-18282 Msa1453530:intron 40.0%
AGATGGAGAAGAATCTCAAG+AGG + tig0034754:18326-18345 None:intergenic 40.0%
ACCTTATCCTCAGCAAGTAA+CGG + tig0034754:18350-18369 None:intergenic 40.0%
GCTATGCATAACAAATAGCG+GGG + tig0034754:18565-18584 None:intergenic 40.0%
TGTGTTCGTCACATCAAAAC+AGG - tig0034754:19104-19123 Msa1453530:intron 40.0%
TCACATCAAAACAGGCACTT+GGG - tig0034754:19112-19131 Msa1453530:intron 40.0%
AAGCCCATCATACCATCATA+CGG + tig0034754:19373-19392 None:intergenic 40.0%
GTAACACTCATGCATCATAC+AGG + tig0034754:19679-19698 None:intergenic 40.0%
ATGAAAGCGGCTAGGGTTTA+TGG + tig0034754:17891-17910 None:intergenic 45.0%
GAAGAGAATGAAAGCGGCTA+GGG + tig0034754:17898-17917 None:intergenic 45.0%
TGGAGAAGCAGTTAACCCTA+AGG - tig0034754:18057-18076 Msa1453530:three_prime_UTR 45.0%
TAACCCTAAGGCATACCCTT+TGG - tig0034754:18069-18088 Msa1453530:three_prime_UTR 45.0%
TTAGCTGAGCATCAGCCAAA+GGG + tig0034754:18087-18106 None:intergenic 45.0%
! TCCGTTACTTGCTGAGGATA+AGG - tig0034754:18346-18365 Msa1453530:CDS 45.0%
CCACAACCCAAACATCTGTA+TGG + tig0034754:18613-18632 None:intergenic 45.0%
!! CCATACAGATGTTTGGGTTG+TGG - tig0034754:18610-18629 Msa1453530:intron 45.0%
CGAGACCTAACAACATTCAG+AGG + tig0034754:18814-18833 None:intergenic 45.0%
GTCACATCAAAACAGGCACT+TGG - tig0034754:19111-19130 Msa1453530:intron 45.0%
AGAACAAGCAATCACTGGTC+GGG + tig0034754:19154-19173 None:intergenic 45.0%
CAGAACAAGCAATCACTGGT+CGG + tig0034754:19155-19174 None:intergenic 45.0%
GTCACAGAACAAGCAATCAC+TGG + tig0034754:19159-19178 None:intergenic 45.0%
GTCTCAGATTCAGCAACTCA+AGG - tig0034754:19203-19222 Msa1453530:CDS 45.0%
!! AGTTGTGCTGAAAGCGTCTT+CGG - tig0034754:19397-19416 Msa1453530:CDS 45.0%
CACACAATAACAACCTTCGG+CGG + tig0034754:19491-19510 None:intergenic 45.0%
ATGAAAGCGGCTAGGGTTTA+TGG + tig0034754:17891-17910 None:intergenic 45.0%
GAAGAGAATGAAAGCGGCTA+GGG + tig0034754:17898-17917 None:intergenic 45.0%
TGGAGAAGCAGTTAACCCTA+AGG - tig0034754:18057-18076 Msa1453530:three_prime_UTR 45.0%
TAACCCTAAGGCATACCCTT+TGG - tig0034754:18069-18088 Msa1453530:three_prime_UTR 45.0%
TTAGCTGAGCATCAGCCAAA+GGG + tig0034754:18087-18106 None:intergenic 45.0%
! TCCGTTACTTGCTGAGGATA+AGG - tig0034754:18346-18365 Msa1453530:CDS 45.0%
CCACAACCCAAACATCTGTA+TGG + tig0034754:18613-18632 None:intergenic 45.0%
!! CCATACAGATGTTTGGGTTG+TGG - tig0034754:18610-18629 Msa1453530:intron 45.0%
CGAGACCTAACAACATTCAG+AGG + tig0034754:18814-18833 None:intergenic 45.0%
GTCACATCAAAACAGGCACT+TGG - tig0034754:19111-19130 Msa1453530:intron 45.0%
AGAACAAGCAATCACTGGTC+GGG + tig0034754:19154-19173 None:intergenic 45.0%
CAGAACAAGCAATCACTGGT+CGG + tig0034754:19155-19174 None:intergenic 45.0%
GTCACAGAACAAGCAATCAC+TGG + tig0034754:19159-19178 None:intergenic 45.0%
GTCTCAGATTCAGCAACTCA+AGG - tig0034754:19203-19222 Msa1453530:CDS 45.0%
!! AGTTGTGCTGAAAGCGTCTT+CGG - tig0034754:19397-19416 Msa1453530:CDS 45.0%
CACACAATAACAACCTTCGG+CGG + tig0034754:19491-19510 None:intergenic 45.0%
CGAAGAGAATGAAAGCGGCT+AGG + tig0034754:17899-17918 None:intergenic 50.0%
! CAGCCAAAGGGTATGCCTTA+GGG + tig0034754:18075-18094 None:intergenic 50.0%
! TCAGCCAAAGGGTATGCCTT+AGG + tig0034754:18076-18095 None:intergenic 50.0%
GTTAGCTGAGCATCAGCCAA+AGG + tig0034754:18088-18107 None:intergenic 50.0%
! CCTCAGCAAGTAACGGAAGA+TGG + tig0034754:18343-18362 None:intergenic 50.0%
! CCATCTTCCGTTACTTGCTG+AGG - tig0034754:18340-18359 Msa1453530:CDS 50.0%
CACAACAAGTCGTCCAGTCA+AGG + tig0034754:19426-19445 None:intergenic 50.0%
! CGCCGAAGGTTGTTATTGTG+TGG - tig0034754:19489-19508 Msa1453530:intron 50.0%
CGAAGAGAATGAAAGCGGCT+AGG + tig0034754:17899-17918 None:intergenic 50.0%
! CAGCCAAAGGGTATGCCTTA+GGG + tig0034754:18075-18094 None:intergenic 50.0%
! TCAGCCAAAGGGTATGCCTT+AGG + tig0034754:18076-18095 None:intergenic 50.0%
GTTAGCTGAGCATCAGCCAA+AGG + tig0034754:18088-18107 None:intergenic 50.0%
! CCTCAGCAAGTAACGGAAGA+TGG + tig0034754:18343-18362 None:intergenic 50.0%
! CCATCTTCCGTTACTTGCTG+AGG - tig0034754:18340-18359 Msa1453530:CDS 50.0%
CACAACAAGTCGTCCAGTCA+AGG + tig0034754:19426-19445 None:intergenic 50.0%
! CGCCGAAGGTTGTTATTGTG+TGG - tig0034754:19489-19508 Msa1453530:intron 50.0%
!! TGTGCTGAAAGCGTCTTCGG+AGG - tig0034754:19400-19419 Msa1453530:CDS 55.0%
!! TGTGCTGAAAGCGTCTTCGG+AGG - tig0034754:19400-19419 Msa1453530:CDS 55.0%
CAGGCACTTGGGCGTGCTTG+TGG - tig0034754:19123-19142 Msa1453530:intron 65.0%
GCGTCTTCGGAGGCCTTGAC+TGG - tig0034754:19410-19429 Msa1453530:CDS 65.0%
CAGGCACTTGGGCGTGCTTG+TGG - tig0034754:19123-19142 Msa1453530:intron 65.0%
GCGTCTTCGGAGGCCTTGAC+TGG - tig0034754:19410-19429 Msa1453530:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0034754 gene 17829 19732 17829 ID=Msa1453530;Name=Msa1453530
tig0034754 mRNA 17829 19732 17829 ID=Msa1453530-mRNA-1;Parent=Msa1453530;Name=Msa1453530-mRNA-1;_AED=0.00;_eAED=0.00;_QI=108|1|1|1|0.75|0.8|5|375|128
tig0034754 exon 17829 18230 17829 ID=Msa1453530-mRNA-1:exon:19188;Parent=Msa1453530-mRNA-1
tig0034754 exon 18337 18468 18337 ID=Msa1453530-mRNA-1:exon:19187;Parent=Msa1453530-mRNA-1
tig0034754 exon 19194 19297 19194 ID=Msa1453530-mRNA-1:exon:19186;Parent=Msa1453530-mRNA-1
tig0034754 exon 19386 19506 19386 ID=Msa1453530-mRNA-1:exon:19185;Parent=Msa1453530-mRNA-1
tig0034754 exon 19622 19732 19622 ID=Msa1453530-mRNA-1:exon:19184;Parent=Msa1453530-mRNA-1
tig0034754 five_prime_UTR 19625 19732 19625 ID=Msa1453530-mRNA-1:five_prime_utr;Parent=Msa1453530-mRNA-1
tig0034754 CDS 19622 19624 19622 ID=Msa1453530-mRNA-1:cds;Parent=Msa1453530-mRNA-1
tig0034754 CDS 19386 19506 19386 ID=Msa1453530-mRNA-1:cds;Parent=Msa1453530-mRNA-1
tig0034754 CDS 19194 19297 19194 ID=Msa1453530-mRNA-1:cds;Parent=Msa1453530-mRNA-1
tig0034754 CDS 18337 18468 18337 ID=Msa1453530-mRNA-1:cds;Parent=Msa1453530-mRNA-1
tig0034754 CDS 18204 18230 18204 ID=Msa1453530-mRNA-1:cds;Parent=Msa1453530-mRNA-1
tig0034754 three_prime_UTR 17829 18203 17829 ID=Msa1453530-mRNA-1:three_prime_utr;Parent=Msa1453530-mRNA-1
Gene Sequence

>Msa1453530

ATGACTGGAGAAGCAGTTAACCCTAAGGCATACCCTTTGGCTGATGCTCAGCTAACAATCACCATAATGGATCTTGTTCAACAAGCTGCTAACTATAAGCAACTCAAAAAGGGTGCTAATGAAGCTACTAAGACCTTGAATAGGGGTATTTCTGAATTTGTTGTTATGGCTGCTGATGCTGAACCTCTTGAGATTCTTCTCCATCTTCCGTTACTTGCTGAGGATAAGAATGTTCCCTATGTGTTCGTCACATCAAAACAGGCACTTGGGCGTGCTTGTGGTGTCACCCGACCAGTGATTGCTTGTTCTGTGACAACTAATGAAGGTAGTCAATTGAAGTCTCAGATTCAGCAACTCAAGGATGCTATTGAGAAGCTGCTAATCTGA
Protein sequence

>Msa1453530

MTGEAVNPKAYPLADAQLTITIMDLVQQAANYKQLKKGANEATKTLNRGISEFVVMAADAEPLEILLHLPLLAEDKNVPYVFVTSKQALGRACGVTRPVIACSVTTNEGSQLKSQIQQLKDAIEKLLI*