AlfalfaGEDB | Msa1249640

M. sativa cultivar zhongmu-4 / Msa1249640

KEGG: K18757
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005844 GO:0007275 GO:0007568 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0009751 GO:0009753 GO:0010033 GO:0010150 GO:0014070 GO:0032501 GO:0032502 GO:0032991 GO:0033993 GO:0042221 GO:0042493 GO:0044424 GO:0044464 GO:0046677 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:1901363 GO:1901700 GO:1990904
PFAM: PF05383.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249640	XP_003629259.1	95.758	165	7	0	14	178	290	454	1.64e-108	327

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249640	sp\|Q8RWR2\|LRP1B_ARATH	43.511	131	62	6	12	135	286	411	2.17e-22	95.9

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249640	G7LB62	95.758	165	7	0	14	178	290	454	7.84e-109	327

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0013050	Msa1249640	0.844910	5.639641e-59	-8.615850e-47
Msa0036220	Msa1249640	0.827293	1.694624e-54	-8.615850e-47
Msa0037570	Msa1249640	0.804633	1.967024e-49	-8.615850e-47
Msa0039330	Msa1249640	-0.802283	6.038319e-49	-8.615850e-47
Msa0049650	Msa1249640	0.814845	1.252115e-51	-8.615850e-47
Msa0054730	Msa1249640	0.858581	7.469486e-63	-8.615850e-47
Msa0057680	Msa1249640	0.810516	1.108823e-50	-8.615850e-47
Msa0074950	Msa1249640	0.835917	1.269394e-56	-8.615850e-47
Msa0076320	Msa1249640	-0.804807	1.809305e-49	-8.615850e-47
Msa0090770	Msa1249640	0.804833	1.786478e-49	-8.615850e-47
Msa0092990	Msa1249640	-0.809583	1.761525e-50	-8.615850e-47
Msa0106370	Msa1249640	0.823593	1.274233e-53	-8.615850e-47
Msa0605170	Msa1249640	-0.813535	2.437059e-51	-8.615850e-47
Msa0607340	Msa1249640	0.842240	2.917134e-58	-8.615850e-47
Msa0626090	Msa1249640	-0.809526	1.811595e-50	-8.615850e-47
Msa0647450	Msa1249640	0.837227	5.887986e-57	-8.615850e-47
Msa0661190	Msa1249640	0.875804	2.308737e-68	-8.615850e-47
Msa0662650	Msa1249640	-0.802370	5.794568e-49	-8.615850e-47
Msa0682840	Msa1249640	-0.805725	1.162049e-49	-8.615850e-47
Msa0708860	Msa1249640	-0.801084	1.063920e-48	-8.615850e-47
Msa0134110	Msa1249640	0.802261	6.099912e-49	-8.615850e-47
Msa0162770	Msa1249640	0.824909	6.251310e-54	-8.615850e-47
Msa0183640	Msa1249640	0.810633	1.046053e-50	-8.615850e-47
Msa1249640	Msa1254370	0.989868	5.256771e-180	-8.615850e-47
Msa1249640	Msa1259590	0.835652	1.481178e-56	-8.615850e-47
Msa1249640	Msa1294610	0.947054	1.416233e-105	-8.615850e-47
Msa1249640	Msa1299290	0.803235	3.840268e-49	-8.615850e-47
Msa1249640	Msa1307660	0.837166	6.101409e-57	-8.615850e-47
Msa1249640	Msa1310770	0.828475	8.809216e-55	-8.615850e-47
Msa1249640	Msa1332040	0.857050	2.124581e-62	-8.615850e-47
Msa1249640	Msa1332280	0.802484	5.487908e-49	-8.615850e-47
Msa1249640	Msa1362880	-0.806614	7.553379e-50	-8.615850e-47
Msa1249640	Msa1365700	0.835069	2.079362e-56	-8.615850e-47
Msa1249640	Msa1395230	0.823441	1.383228e-53	-8.615850e-47
Msa1249640	Msa1403930	0.818359	2.043029e-52	-8.615850e-47
Msa1249640	Msa1410430	0.822015	2.969705e-53	-8.615850e-47
Msa1249640	Msa1426030	-0.806272	8.917726e-50	-8.615850e-47
Msa1249640	Msa1442160	0.816053	6.743825e-52	-8.615850e-47
Msa1249640	Msa1445610	0.811433	7.018984e-51	-8.615850e-47
Msa1249640	Msa1449250	0.800707	1.270054e-48	-8.615850e-47
Msa1249640	Msa1453780	0.886446	3.396107e-72	-8.615850e-47
Msa0467900	Msa1249640	0.813846	2.081531e-51	-8.615850e-47
Msa0993290	Msa1249640	-0.810073	1.382032e-50	-8.615850e-47
Msa0993600	Msa1249640	-0.802591	5.217199e-49	-8.615850e-47
Msa1030880	Msa1249640	0.821782	3.362480e-53	-8.615850e-47
Msa1033410	Msa1249640	0.815540	8.774042e-52	-8.615850e-47
Msa1039880	Msa1249640	-0.806061	9.875003e-50	-8.615850e-47
Msa1046020	Msa1249640	-0.808673	2.758646e-50	-8.615850e-47
Msa1046060	Msa1249640	-0.804958	1.682438e-49	-8.615850e-47
Msa1072460	Msa1249640	0.820165	7.923575e-53	-8.615850e-47
Msa0260950	Msa1249640	0.802369	5.798030e-49	-8.615850e-47
Msa1143930	Msa1249640	0.801171	1.021259e-48	-8.615850e-47
Msa1177260	Msa1249640	0.814502	1.490910e-51	-8.615850e-47
Msa1181550	Msa1249640	-0.811163	8.030873e-51	-8.615850e-47
Msa1201430	Msa1249640	0.955372	3.527028e-113	-8.615850e-47
Msa1211140	Msa1249640	0.806419	8.302274e-50	-8.615850e-47
Msa0512750	Msa1249640	-0.812038	5.183084e-51	-8.615850e-47
Msa0528020	Msa1249640	0.806702	7.234306e-50	-8.615850e-47
Msa0539000	Msa1249640	0.807628	4.605461e-50	-8.615850e-47
Msa0553220	Msa1249640	0.851610	7.936354e-61	-8.615850e-47
Msa0563260	Msa1249640	0.825005	5.934404e-54	-8.615850e-47
Msa0585000	Msa1249640	0.852720	3.834970e-61	-8.615850e-47
Msa0867050	Msa1249640	0.821257	4.446005e-53	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249640	MtrunA17_Chr8g0371401	95.758	165	7	0	14	178	290	454	1.51e-112	327
Msa1249640	MtrunA17_Chr5g0405961	52.717	184	72	8	2	172	296	477	2.90e-49	166
Msa1249640	MtrunA17_Chr1g0157011	47.799	159	76	3	10	165	362	516	1.90e-37	135
Msa1249640	MtrunA17_Chr3g0143261	43.671	158	78	4	9	159	314	467	1.04e-33	124
Msa1249640	MtrunA17_Chr3g0143171	48.092	131	58	3	9	133	206	332	1.90e-32	119
Msa1249640	MtrunA17_Chr5g0440821	40.909	154	81	3	36	179	290	443	1.87e-26	105
Msa1249640	MtrunA17_Chr7g0276671	39.048	105	60	2	33	133	88	192	2.84e-15	72.8
Msa1249640	MtrunA17_Chr4g0045341	40.741	81	44	3	58	134	98	178	2.49e-12	64.3
Msa1249640	MtrunA17_Chr2g0286061	37.143	105	58	3	58	158	102	202	5.54e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1249640	AT5G66100.1	43.511	131	62	6	12	135	287	412	2.09e-23	96.3
Msa1249640	AT4G35890.1	47.573	103	48	2	40	136	348	450	1.78e-22	94.0
Msa1249640	AT5G21160.2	41.441	111	58	3	36	141	256	364	2.27e-21	90.9
Msa1249640	AT5G21160.3	41.441	111	58	3	36	141	256	364	2.27e-21	90.9
Msa1249640	AT5G21160.1	41.441	111	58	3	36	141	256	364	2.38e-21	90.9
Msa1249640	AT5G46250.2	41.667	84	41	3	55	132	99	180	1.47e-11	62.0
Msa1249640	AT5G46250.1	41.667	84	41	3	55	132	99	180	1.51e-11	62.4
Msa1249640	AT5G46250.3	41.667	84	41	3	55	132	99	180	1.56e-11	62.0
Msa1249640	AT2G43970.1	39.759	83	46	2	62	140	196	278	4.25e-11	61.2
Msa1249640	AT2G43970.2	39.759	83	46	2	62	140	196	278	4.73e-11	60.8

Find 165 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCGCTTAATTGCAATTT+TGG	0.104445	8_2:+44587995	Msa1249640:three_prime_UTR
CCACCCAATCCCACCTTATA+TGG	0.173339	8_2:+44586095	Msa1249640:CDS
ATCTCCGTAGCAAAATAATT+TGG	0.211790	8_2:-44584935	None:intergenic
AATCCTGCTATTAAATTTAG+AGG	0.222995	8_2:-44586530	None:intergenic
TGGCCATTGGATTGGGAATC+AGG	0.243284	8_2:-44584963	None:intergenic
ACCAGATTGACTACTATTTC+AGG	0.266021	8_2:+44586342	Msa1249640:CDS
TTGTTTCCAGATCAATGTTT+TGG	0.295458	8_2:-44587803	None:intergenic
TTTGGTTTGAGAGTGAATTA+AGG	0.309884	8_2:-44584917	None:intergenic
CTGTGAATCGATGGAAATAA+AGG	0.315887	8_2:-44588020	None:intergenic
TGGCGGCTGGCGGCCTCCAT+TGG	0.323639	8_2:-44585733	None:intergenic
CTCCAATGCTGCTGCTTCTT+CGG	0.327782	8_2:+44585176	Msa1249640:five_prime_UTR
GTCTGCTTCCAGAGTTAAAA+TGG	0.329463	8_2:-44585932	None:intergenic
TCCGGTTCCTGGATGAATTT+CGG	0.329667	8_2:-44585872	None:intergenic
TATAACATGTGACTGATTAA+TGG	0.335264	8_2:+44587962	Msa1249640:three_prime_UTR
ATAAAATAAGGAGGCGGAAT+TGG	0.343985	8_2:+44587712	Msa1249640:CDS
CCACCTTATATGGGCTCATA+TGG	0.349352	8_2:+44586105	Msa1249640:CDS
CAATCATAACTCTGCTCAAA+TGG	0.349628	8_2:+44585803	Msa1249640:five_prime_UTR
TGGCGATTTCGCCGGAGCTT+TGG	0.359704	8_2:-44585238	None:intergenic
CTGGCTCCAAGGTGCGGCGG+CGG	0.361110	8_2:-44585001	None:intergenic
TTAACGGTAGAGACAATTAT+AGG	0.363096	8_2:+44586003	Msa1249640:five_prime_UTR
GAAGAGGAACATGGATGATC+AGG	0.368604	8_2:+44586499	Msa1249640:CDS
CCAAGGTGCGGCGGCGGGAG+AGG	0.373375	8_2:-44584995	None:intergenic
TATAAGGTGGGATTGGGTGG+CGG	0.373504	8_2:-44586092	None:intergenic
GTTGTAGACCACCGCCATTC+CGG	0.378736	8_2:-44585890	None:intergenic
CGGCGCTGAGGCTGATCATT+GGG	0.381172	8_2:-44585852	None:intergenic
GATTTGTTCCACAGTTTGTA+AGG	0.390687	8_2:+44586033	Msa1249640:five_prime_UTR
ACCACCGCCATTCCGGTTCC+TGG	0.392452	8_2:-44585883	None:intergenic
ATAAGGTGGGATTGGGTGGC+GGG	0.395379	8_2:-44586091	None:intergenic
CCTCTCCCGCCGCCGCACCT+TGG	0.396404	8_2:+44584995	Msa1249640:five_prime_UTR
CTCTGGAAGCAGACGTGATC+AGG	0.400093	8_2:+44585941	Msa1249640:five_prime_UTR
TATCCATATGAGCCCATATA+AGG	0.400182	8_2:-44586108	None:intergenic
ACAAACTGTGGAACAAATCT+TGG	0.401130	8_2:-44586029	None:intergenic
CAACTCATCAGAAAACTTAT+CGG	0.402114	8_2:+44585688	Msa1249640:five_prime_UTR
GCGTTCATGGCCATTGGATT+GGG	0.402955	8_2:-44584970	None:intergenic
GGTGGTGATGGCGGTGGTGG+TGG	0.410660	8_2:-44585258	None:intergenic
GCCATCACCACCACAGGAAT+TGG	0.412805	8_2:+44585269	Msa1249640:five_prime_UTR
GCGGCGGAGGAAGGAGGAGG+AGG	0.419591	8_2:-44585072	None:intergenic
TGATCAGGACCGCGGAAATC+AGG	0.421066	8_2:+44585956	Msa1249640:five_prime_UTR
AGCGTTCATGGCCATTGGAT+TGG	0.423387	8_2:-44584971	None:intergenic
TGTGGTGGTGATGGCGGTGG+TGG	0.424964	8_2:-44585261	None:intergenic
TGCTGCTGCTTCTTCGGTCA+TGG	0.429405	8_2:+44585182	Msa1249640:five_prime_UTR
TAAGGTGGGATTGGGTGGCG+GGG	0.431950	8_2:-44586090	None:intergenic
GAATATCCAAAACATTGATC+TGG	0.432389	8_2:+44587797	Msa1249640:CDS
GGCGGGAGAGGAAGCGTTCA+TGG	0.432822	8_2:-44584983	None:intergenic
CTCTCCTCAGAAGCAAGTTA+AGG	0.433186	8_2:+44585632	Msa1249640:five_prime_UTR
TGCACAAACGGTACACTTCT+CGG	0.433948	8_2:-44586257	None:intergenic
CGATTTCGCCGGAGCTTTGG+CGG	0.441633	8_2:-44585235	None:intergenic
GGAATTGGTGAAACCTTCCT+TGG	0.443310	8_2:+44585284	Msa1249640:five_prime_UTR
ACCTGAAATAGTAGTCAATC+TGG	0.445908	8_2:-44586343	None:intergenic
ATCTGAATCCGCCAAAGCTC+CGG	0.446417	8_2:+44585227	Msa1249640:five_prime_UTR
TGACAACATTCAGATTGTAT+TGG	0.447780	8_2:+44587516	Msa1249640:CDS
TTCATCTGTTGTTGAAGTGC+AGG	0.450747	8_2:+44587552	Msa1249640:CDS
GAAGACATCGACAACACTTC+CGG	0.453122	8_2:+44585126	Msa1249640:five_prime_UTR
ACAGCGGCGGAGGAAGGAGG+AGG	0.454286	8_2:-44585075	None:intergenic
TTGGAGGGTTTACTCCAAAC+AGG	0.455281	8_2:-44585159	None:intergenic
TCACAGTTTCATAACGTCAA+TGG	0.458834	8_2:+44587747	Msa1249640:CDS
CAGCGGTGGAGGCAACAATC+TGG	0.459815	8_2:-44585020	None:intergenic
TGGCTCCAAGGTGCGGCGGC+GGG	0.460627	8_2:-44585000	None:intergenic
GCCGAAATTCATCCAGGAAC+CGG	0.461700	8_2:+44585871	Msa1249640:five_prime_UTR
GTTCCTCTAAATTTAATAGC+AGG	0.461861	8_2:+44586527	Msa1249640:CDS
GGTTTCGTTGCTGTCCCACC+AGG	0.463812	8_2:+44585774	Msa1249640:five_prime_UTR
GACCGAAGAAGCAGCAGCAT+TGG	0.465776	8_2:-44585178	None:intergenic
GAGGAAGCGTTCATGGCCAT+TGG	0.466060	8_2:-44584976	None:intergenic
GAAGATATCGGTTGCACAAA+CGG	0.466726	8_2:-44586269	None:intergenic
CCTTGAGGCGCAGACATTTG+TGG	0.469144	8_2:-44585753	None:intergenic
TAGTATTGGGCATGATTAGT+AGG	0.473060	8_2:-44586065	None:intergenic
GAGCCCATATAAGGTGGGAT+TGG	0.473842	8_2:-44586099	None:intergenic
AGCCCATATAAGGTGGGATT+GGG	0.475161	8_2:-44586098	None:intergenic
AAGGTGGGATTGGGTGGCGG+GGG	0.476178	8_2:-44586089	None:intergenic
CACCCAATCCCACCTTATAT+GGG	0.477885	8_2:+44586096	Msa1249640:CDS
TTGAGCAGAGTTATGATTGT+GGG	0.478241	8_2:-44585799	None:intergenic
ATCGACCTTGCCTCAATCGC+AGG	0.478474	8_2:+44585311	Msa1249640:five_prime_UTR
ATGGGCTCATATGGATATCA+TGG	0.478647	8_2:+44586114	Msa1249640:CDS
CGAAGAAGCAGCAGCATTGG+AGG	0.481797	8_2:-44585175	None:intergenic
TACGGCGGAAGCAGCGGTGG+AGG	0.484516	8_2:-44585031	None:intergenic
TTAGTATGCAACTCAGAATC+CGG	0.489983	8_2:-44586311	None:intergenic
GAGGAAGGAGGAGGAGGTGA+CGG	0.494008	8_2:-44585066	None:intergenic
GGAACATGGATGATCAGGGC+TGG	0.494066	8_2:+44586504	Msa1249640:CDS
GGCCATTGGATTGGGAATCA+GGG	0.495667	8_2:-44584962	None:intergenic
CTAACGCTTCAAATGATGTA+TGG	0.503281	8_2:+44586212	Msa1249640:CDS
GAAAGTGTCGACAGCGGCGG+AGG	0.507299	8_2:-44585085	None:intergenic
CGGTCATGGATGCTGACTCA+TGG	0.507318	8_2:+44585196	Msa1249640:five_prime_UTR
ACCAATTCCTGTGGTGGTGA+TGG	0.511057	8_2:-44585270	None:intergenic
GTGTCGACAGCGGCGGAGGA+AGG	0.512183	8_2:-44585081	None:intergenic
GTCTTCCGCCGCCTCAGTGA+CGG	0.512716	8_2:-44585109	None:intergenic
TCCTGTGGTGGTGATGGCGG+TGG	0.513238	8_2:-44585264	None:intergenic
TTGGGCACAAATCCAGCTCT+TGG	0.515928	8_2:-44585834	None:intergenic
GTATCCTTAACTTGCTTCTG+AGG	0.520478	8_2:-44585636	None:intergenic
ACCACCGCCATCACCACCAC+AGG	0.524419	8_2:+44585263	Msa1249640:five_prime_UTR
CATACATCATTTGAAGCGTT+AGG	0.525907	8_2:-44586211	None:intergenic
TCCAGGAACCGGAATGGCGG+TGG	0.529103	8_2:+44585882	Msa1249640:five_prime_UTR
AGGTACGGCGGAAGCAGCGG+TGG	0.532026	8_2:-44585034	None:intergenic
TGGAGAATATTGTTCGGATG+AGG	0.532604	8_2:+44587896	Msa1249640:three_prime_UTR
CCACAAATGTCTGCGCCTCA+AGG	0.534512	8_2:+44585753	Msa1249640:five_prime_UTR
ATGGAACATCTACCAAGAGC+TGG	0.535798	8_2:+44585822	Msa1249640:five_prime_UTR
CCATATAAGGTGGGATTGGG+TGG	0.539136	8_2:-44586095	None:intergenic
GCATGATTAGTAGGCGGTGG+AGG	0.541418	8_2:-44586056	None:intergenic
AATTCAAACACGTCTTCCAA+TGG	0.542364	8_2:+44585717	Msa1249640:five_prime_UTR
GGTGGTGGTGGCGATTTCGC+CGG	0.543934	8_2:-44585246	None:intergenic
GAACATGGATGATCAGGGCT+GGG	0.545097	8_2:+44586505	Msa1249640:CDS
CAGACATTTGTGGCGGCTGG+CGG	0.546833	8_2:-44585743	None:intergenic
TGTTCGGATGAGGCAGGAAG+CGG	0.550019	8_2:+44587906	Msa1249640:three_prime_UTR
TGCAACTCAGAATCCGGAGG+TGG	0.551141	8_2:-44586305	None:intergenic
GTTCGGATGAGGCAGGAAGC+GGG	0.552139	8_2:+44587907	Msa1249640:three_prime_UTR
GCAACGACACATACTTGAAG+AGG	0.552646	8_2:+44586483	Msa1249640:CDS
GAATATTGTTCGGATGAGGC+AGG	0.553635	8_2:+44587900	Msa1249640:three_prime_UTR
CTCGGAGGCTCCACATGCAA+AGG	0.554785	8_2:-44586239	None:intergenic
GTACTAAAACTGTGAATCGA+TGG	0.557881	8_2:-44588029	None:intergenic
TCGACAGCGGCGGAGGAAGG+AGG	0.564961	8_2:-44585078	None:intergenic
AATAAAACACTTCATAACCT+TGG	0.565964	8_2:+44584886	None:intergenic
CCATATGAGCCCATATAAGG+TGG	0.568642	8_2:-44586105	None:intergenic
AAGCAAGTTAAGGATACTGC+AGG	0.569915	8_2:+44585642	Msa1249640:five_prime_UTR
TTGGCGGATTCAGATAAAGC+AGG	0.573000	8_2:-44585219	None:intergenic
TGGGCATGATTAGTAGGCGG+TGG	0.573030	8_2:-44586059	None:intergenic
GGAGGTGACGGCGATGAATG+AGG	0.573341	8_2:-44585054	None:intergenic
TTTCGCCGTCACTGAGGCGG+CGG	0.577187	8_2:+44585104	Msa1249640:five_prime_UTR
AACAATCTGGCTCCAAGGTG+CGG	0.577674	8_2:-44585007	None:intergenic
GAGGCAACAATCTGGCTCCA+AGG	0.579221	8_2:-44585012	None:intergenic
TTTAGGGTGATAAAATAAGG+AGG	0.579670	8_2:+44587703	Msa1249640:intron
GGTCTACAACCACGCGGAGA+TGG	0.584916	8_2:+44585903	Msa1249640:five_prime_UTR
CCGGTGGTAAGAGACCTGTT+TGG	0.585862	8_2:+44585145	Msa1249640:five_prime_UTR
AAGAGGAACATGGATGATCA+GGG	0.588462	8_2:+44586500	Msa1249640:CDS
GGTTTCACCAATTCCTGTGG+TGG	0.591849	8_2:-44585276	None:intergenic
CACTTTCGCCGTCACTGAGG+CGG	0.594778	8_2:+44585101	Msa1249640:five_prime_UTR
CTGGATGAATTTCGGCGCTG+AGG	0.595383	8_2:-44585864	None:intergenic
CACATACTTGAAGAGGAACA+TGG	0.596708	8_2:+44586490	Msa1249640:CDS
AACCCTGATTCCCAATCCAA+TGG	0.597508	8_2:+44584960	Msa1249640:five_prime_UTR
AGGGTGATAAAATAAGGAGG+CGG	0.599307	8_2:+44587706	Msa1249640:intron
ATGAGGTACGGCGGAAGCAG+CGG	0.604517	8_2:-44585037	None:intergenic
GAGTTATGATTGTGGGACCC+TGG	0.608836	8_2:-44585792	None:intergenic
GTATGGTCCACCTTTGCATG+TGG	0.613005	8_2:+44586229	Msa1249640:CDS
AATTCATCCAGGAACCGGAA+TGG	0.615009	8_2:+44585876	Msa1249640:five_prime_UTR
TGACGGCGATGAATGAGGTA+CGG	0.620582	8_2:-44585049	None:intergenic
GAAGAAGCAGCAGCATTGGA+GGG	0.621002	8_2:-44585174	None:intergenic
CCAAACAGGTCTCTTACCAC+CGG	0.621816	8_2:-44585145	None:intergenic
GGAGGCTCCACATGCAAAGG+TGG	0.622207	8_2:-44586236	None:intergenic
CATATGAGCCCATATAAGGT+GGG	0.622209	8_2:-44586104	None:intergenic
TTTGAGCAGAGTTATGATTG+TGG	0.622984	8_2:-44585800	None:intergenic
CATTCCAGTCCTGATTTCCG+CGG	0.624273	8_2:-44585965	None:intergenic
AAATACCTGCGATTGAGGCA+AGG	0.626925	8_2:-44585316	None:intergenic
TCAAACACGTCTTCCAATGG+AGG	0.628375	8_2:+44585720	Msa1249640:five_prime_UTR
TATGATTGTGGGACCCTGGT+GGG	0.628962	8_2:-44585788	None:intergenic
AGGCAAGGTCGATAAATCCA+AGG	0.629817	8_2:-44585301	None:intergenic
TGAGGCGCAGACATTTGTGG+CGG	0.632231	8_2:-44585750	None:intergenic
GGCGAAAGTGTCGACAGCGG+CGG	0.635606	8_2:-44585088	None:intergenic
GAAGGTTTCACCAATTCCTG+TGG	0.639283	8_2:-44585279	None:intergenic
AAGGTCGATAAATCCAAGGA+AGG	0.645541	8_2:-44585297	None:intergenic
TCATCCAGGAACCGGAATGG+CGG	0.654453	8_2:+44585879	Msa1249640:five_prime_UTR
GTTTCGTTGCTGTCCCACCA+GGG	0.657439	8_2:+44585775	Msa1249640:five_prime_UTR
AATCTGGCTCCAAGGTGCGG+CGG	0.658713	8_2:-44585004	None:intergenic
AATTCCTGTGGTGGTGATGG+CGG	0.664267	8_2:-44585267	None:intergenic
GAATTAAGGAATTTATACCA+AGG	0.667605	8_2:-44584903	None:intergenic
GTATGCAACTCAGAATCCGG+AGG	0.681468	8_2:-44586308	None:intergenic
AGCAGACGTGATCAGGACCG+CGG	0.696086	8_2:+44585948	Msa1249640:five_prime_UTR
GACATCGACAACACTTCCGG+TGG	0.701044	8_2:+44585129	Msa1249640:five_prime_UTR
GACGGCGAAAGTGTCGACAG+CGG	0.707075	8_2:-44585091	None:intergenic
CGACACTTTCGCCGTCACTG+AGG	0.708576	8_2:+44585098	Msa1249640:five_prime_UTR
GGTGGAGGCCTTACAAACTG+TGG	0.712069	8_2:-44586041	None:intergenic
CGGCGATGAATGAGGTACGG+CGG	0.718688	8_2:-44585046	None:intergenic
TATTGGGCATGATTAGTAGG+CGG	0.723347	8_2:-44586062	None:intergenic
GGGACAGCAACGAAACCTTG+AGG	0.727311	8_2:-44585768	None:intergenic
TTATGATTGTGGGACCCTGG+TGG	0.738203	8_2:-44585789	None:intergenic
TGGTGAGAACCATCTCCGCG+TGG	0.763757	8_2:-44585912	None:intergenic
ACAAACGGTACACTTCTCGG+AGG	0.802512	8_2:-44586254	None:intergenic
GGCGGTGGTCTACAACCACG+CGG	0.809396	8_2:+44585897	Msa1249640:five_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGTAATTTTTTTAAATAG+GGG	-	chr8_2:44586850-44586869	None:intergenic	10.0%
!!!	AATTTTACTATTTTTTGATT+TGG	+	chr8_2:44587135-44587154	Msa1249640:intron	10.0%
!!	AAAACAATATATTTATGATA+TGG	-	chr8_2:44588183-44588202	None:intergenic	10.0%
!!	CAAAACAATAAAATGAAAAT+TGG	-	chr8_2:44586391-44586410	None:intergenic	15.0%
!!!	TTTTAATTAAATCTTTGACA+TGG	+	chr8_2:44586591-44586610	Msa1249640:intron	15.0%
!!	TTTAATTAAATCTTTGACAT+GGG	+	chr8_2:44586592-44586611	Msa1249640:intron	15.0%
!!	ATTCAATAACAATTTGTATT+AGG	+	chr8_2:44586779-44586798	Msa1249640:intron	15.0%
!!!	TTGTAATTTTTTTAAATAGG+GGG	-	chr8_2:44586849-44586868	None:intergenic	15.0%
!!	TCAAAAGATTATTAATACAA+AGG	-	chr8_2:44587100-44587119	None:intergenic	15.0%
!!!	GTATTAATAATCTTTTGATA+TGG	+	chr8_2:44587102-44587121	Msa1249640:intron	15.0%
!!	TGATAATATCAATTATAATG+TGG	+	chr8_2:44587428-44587447	Msa1249640:intron	15.0%
!!	GATAATATCAATTATAATGT+GGG	+	chr8_2:44587429-44587448	Msa1249640:intron	15.0%
!!!	AATCTGTTACTTTTATTTTT+AGG	+	chr8_2:44587686-44587705	Msa1249640:intron	15.0%
!!!	ATCTGTTACTTTTATTTTTA+GGG	+	chr8_2:44587687-44587706	Msa1249640:intron	15.0%
!!!	CTTTTCTTAAACAATTTTAA+TGG	+	chr8_2:44587876-44587895	Msa1249640:three_prime_UTR	15.0%
!!!	ATATTTTCAGATTTGAAAAT+GGG	-	chr8_2:44588054-44588073	None:intergenic	15.0%
!!!	TATATTTTCAGATTTGAAAA+TGG	-	chr8_2:44588055-44588074	None:intergenic	15.0%
!!	AATAAATAAGCAACTAAAGT+GGG	-	chr8_2:44585348-44585367	None:intergenic	20.0%
!!	TAATAAATAAGCAACTAAAG+TGG	-	chr8_2:44585349-44585368	None:intergenic	20.0%
!!!	TTCCTTTATTTATCAAGTTA+GGG	+	chr8_2:44585401-44585420	Msa1249640:intron	20.0%
!!	GCATATAACTGAAATATATT+TGG	+	chr8_2:44585458-44585477	Msa1249640:intron	20.0%
!!!	AATATATTTGGAATTGTTAC+TGG	+	chr8_2:44585470-44585489	Msa1249640:intron	20.0%
!!!	GCTCATAATAGAAATTTTAA+CGG	+	chr8_2:44585987-44586006	Msa1249640:five_prime_UTR	20.0%
!!	AAAAATATTGCAGAAGATAT+CGG	-	chr8_2:44586284-44586303	None:intergenic	20.0%
!!	ATTCAATAAAGTAAGTTGTT+TGG	+	chr8_2:44586550-44586569	Msa1249640:intron	20.0%
!!!	TTTTGTCATTTTCTTTCATA+GGG	+	chr8_2:44587023-44587042	Msa1249640:intron	20.0%
!!!	ATTTTTAGGGTGATAAAATA+AGG	+	chr8_2:44587700-44587719	Msa1249640:intron	20.0%
!!!	TTTTAATGGAGAATATTGTT+CGG	+	chr8_2:44587890-44587909	Msa1249640:three_prime_UTR	20.0%
!!!	AAAAAAAAAACTTCTAAAGC+TGG	-	chr8_2:44588083-44588102	None:intergenic	20.0%
!	GAATTAAGGAATTTATACCA+AGG	-	chr8_2:44584906-44584925	None:intergenic	25.0%
!!!	CAAACCAAATTATTTTGCTA+CGG	+	chr8_2:44584931-44584950	Msa1249640:five_prime_UTR	25.0%
!!	GTTCCTTTATTTATCAAGTT+AGG	+	chr8_2:44585400-44585419	Msa1249640:intron	25.0%
!	AACCCTAACTTGATAAATAA+AGG	-	chr8_2:44585406-44585425	None:intergenic	25.0%
!!!	TTCTGTTTTTGTTATGTTTG+TGG	+	chr8_2:44585549-44585568	Msa1249640:intron	25.0%
!!!	TTGTTTTGAGTGTTGATTTT+AGG	+	chr8_2:44585590-44585609	Msa1249640:intron	25.0%
!!!	TGTTTTGAGTGTTGATTTTA+GGG	+	chr8_2:44585591-44585610	Msa1249640:intron	25.0%
!	AATCCTGCTATTAAATTTAG+AGG	-	chr8_2:44586533-44586552	None:intergenic	25.0%
!	TGACATAATCAATTTATCGT+TGG	+	chr8_2:44586665-44586684	Msa1249640:intron	25.0%
!!	TTAATCAATATCTTTTACCC+TGG	+	chr8_2:44586709-44586728	Msa1249640:intron	25.0%
!!!	AAAATCGCAACTTTTTGAAA+CGG	-	chr8_2:44586824-44586843	None:intergenic	25.0%
!	ATCATATTTCCCTTATGTTA+GGG	+	chr8_2:44586966-44586985	Msa1249640:intron	25.0%
!!!	CTTTTGTCATTTTCTTTCAT+AGG	+	chr8_2:44587022-44587041	Msa1249640:intron	25.0%
!!!	TTTGTCATTTTCTTTCATAG+GGG	+	chr8_2:44587024-44587043	Msa1249640:intron	25.0%
!	TAGAAGAACAATCATAAAAG+AGG	-	chr8_2:44587184-44587203	None:intergenic	25.0%
!	CGATAAAATTAGAAAATGAG+TGG	+	chr8_2:44587214-44587233	Msa1249640:intron	25.0%
!	TAAAATTAGAAAATGAGTGG+AGG	+	chr8_2:44587217-44587236	Msa1249640:intron	25.0%
!	AAGTGAAAATCAAAAGGATT+TGG	-	chr8_2:44587325-44587344	None:intergenic	25.0%
!	GTGAAAAAGTGAAAATCAAA+AGG	-	chr8_2:44587331-44587350	None:intergenic	25.0%
!!!	AATGCATTTTTTTGTCTCTT+AGG	+	chr8_2:44587480-44587499	Msa1249640:intron	25.0%
!	GTACTAAATTAGAATGTTTG+TGG	-	chr8_2:44587605-44587624	None:intergenic	25.0%
!	GATCTGGAAACAAATAAAAA+TGG	+	chr8_2:44587813-44587832	Msa1249640:CDS	25.0%
!	TATAACATGTGACTGATTAA+TGG	+	chr8_2:44587962-44587981	Msa1249640:three_prime_UTR	25.0%
!	TATGCATTTCACAAATTCAA+TGG	-	chr8_2:44588132-44588151	None:intergenic	25.0%
!!	TTTGGTTTGAGAGTGAATTA+AGG	-	chr8_2:44584920-44584939	None:intergenic	30.0%
	ATCTCCGTAGCAAAATAATT+TGG	-	chr8_2:44584938-44584957	None:intergenic	30.0%
	CAACTCATCAGAAAACTTAT+CGG	+	chr8_2:44585688-44585707	Msa1249640:five_prime_UTR	30.0%
	TTAACGGTAGAGACAATTAT+AGG	+	chr8_2:44586003-44586022	Msa1249640:five_prime_UTR	30.0%
	TTCAGGTAACGTTTATCTAT+TGG	+	chr8_2:44586359-44586378	Msa1249640:intron	30.0%
	GTTCCTCTAAATTTAATAGC+AGG	+	chr8_2:44586527-44586546	Msa1249640:CDS	30.0%
!!!	AAATCGCAACTTTTTGAAAC+GGG	-	chr8_2:44586823-44586842	None:intergenic	30.0%
!	GTTTCAAAAAGTTGCGATTT+TGG	+	chr8_2:44586823-44586842	Msa1249640:intron	30.0%
!!	GCAACTTTTTGAGAAGATAA+GGG	+	chr8_2:44586935-44586954	Msa1249640:intron	30.0%
!!	CAACTTTTTGAGAAGATAAG+GGG	+	chr8_2:44586936-44586955	Msa1249640:intron	30.0%
	GATCATATTTCCCTTATGTT+AGG	+	chr8_2:44586965-44586984	Msa1249640:intron	30.0%
	TCATATTTCCCTTATGTTAG+GGG	+	chr8_2:44586967-44586986	Msa1249640:intron	30.0%
!!!	TTGTCATTTTCTTTCATAGG+GGG	+	chr8_2:44587025-44587044	Msa1249640:intron	30.0%
!!!	GTTTTAAGAAGTTGCGATTT+TGG	-	chr8_2:44587052-44587071	None:intergenic	30.0%
	TCGCAACTTCTTAAAACTTA+AGG	+	chr8_2:44587055-44587074	Msa1249640:intron	30.0%
	TGACAACATTCAGATTGTAT+TGG	+	chr8_2:44587516-44587535	Msa1249640:CDS	30.0%
!	TTTAGGGTGATAAAATAAGG+AGG	+	chr8_2:44587703-44587722	Msa1249640:intron	30.0%
	GAATATCCAAAACATTGATC+TGG	+	chr8_2:44587797-44587816	Msa1249640:CDS	30.0%
!	TTGTTTCCAGATCAATGTTT+TGG	-	chr8_2:44587806-44587825	None:intergenic	30.0%
	ACAAATAAAAATGGTGCATC+TGG	+	chr8_2:44587822-44587841	Msa1249640:CDS	30.0%
!	TTGTTATGAATTTTCTCACG+AGG	+	chr8_2:44587937-44587956	Msa1249640:three_prime_UTR	30.0%
!	TCTTCGCTTAATTGCAATTT+TGG	+	chr8_2:44587995-44588014	Msa1249640:three_prime_UTR	30.0%
!!	AAAAAAACTTCTAAAGCTGG+AGG	-	chr8_2:44588080-44588099	None:intergenic	30.0%
	GAAAAAAATACCTGCGATTG+AGG	-	chr8_2:44585324-44585343	None:intergenic	35.0%
!	CGATAAGTTTTCTGATGAGT+TGG	-	chr8_2:44585690-44585709	None:intergenic	35.0%
	AATTCAAACACGTCTTCCAA+TGG	+	chr8_2:44585717-44585736	Msa1249640:five_prime_UTR	35.0%
	TTGAGCAGAGTTATGATTGT+GGG	-	chr8_2:44585802-44585821	None:intergenic	35.0%
	TTTGAGCAGAGTTATGATTG+TGG	-	chr8_2:44585803-44585822	None:intergenic	35.0%
	CAATCATAACTCTGCTCAAA+TGG	+	chr8_2:44585803-44585822	Msa1249640:five_prime_UTR	35.0%
	ACAAACTGTGGAACAAATCT+TGG	-	chr8_2:44586032-44586051	None:intergenic	35.0%
!	GATTTGTTCCACAGTTTGTA+AGG	+	chr8_2:44586033-44586052	Msa1249640:five_prime_UTR	35.0%
!	TAGTATTGGGCATGATTAGT+AGG	-	chr8_2:44586068-44586087	None:intergenic	35.0%
	TATCCATATGAGCCCATATA+AGG	-	chr8_2:44586111-44586130	None:intergenic	35.0%
!	CATACATCATTTGAAGCGTT+AGG	-	chr8_2:44586214-44586233	None:intergenic	35.0%
	CTAACGCTTCAAATGATGTA+TGG	+	chr8_2:44586212-44586231	Msa1249640:CDS	35.0%
!!!	TGCAATATTTTTTCCACCTC+CGG	+	chr8_2:44586292-44586311	Msa1249640:CDS	35.0%
	TTAGTATGCAACTCAGAATC+CGG	-	chr8_2:44586314-44586333	None:intergenic	35.0%
	ACCAGATTGACTACTATTTC+AGG	+	chr8_2:44586342-44586361	Msa1249640:CDS	35.0%
	ACCTGAAATAGTAGTCAATC+TGG	-	chr8_2:44586346-44586365	None:intergenic	35.0%
	AACAACGATCTTCAAATCCA+GGG	-	chr8_2:44586729-44586748	None:intergenic	35.0%
!!	CGCAACTTTTTGAGAAGATA+AGG	+	chr8_2:44586934-44586953	Msa1249640:intron	35.0%
!	TCTTTTGACCCCTAACATAA+GGG	-	chr8_2:44586978-44586997	None:intergenic	35.0%
!	AAGAGCATATTTCCCTAAAC+AGG	+	chr8_2:44586994-44587013	Msa1249640:intron	35.0%
	AGAGCATATTTCCCTAAACA+GGG	+	chr8_2:44586995-44587014	Msa1249640:intron	35.0%
!!!	TGTCATTTTCTTTCATAGGG+GGG	+	chr8_2:44587026-44587045	Msa1249640:intron	35.0%
	ATAAAATAAGGAGGCGGAAT+TGG	+	chr8_2:44587712-44587731	Msa1249640:CDS	35.0%
	TCACAGTTTCATAACGTCAA+TGG	+	chr8_2:44587747-44587766	Msa1249640:CDS	35.0%
	CTGTGAATCGATGGAAATAA+AGG	-	chr8_2:44588023-44588042	None:intergenic	35.0%
	GTACTAAAACTGTGAATCGA+TGG	-	chr8_2:44588032-44588051	None:intergenic	35.0%
	GCATTTCACAAATTCAATGG+TGG	-	chr8_2:44588129-44588148	None:intergenic	35.0%
	AAGGTCGATAAATCCAAGGA+AGG	-	chr8_2:44585300-44585319	None:intergenic	40.0%
!!!	GAGTGTTGATTTTAGGGTAC+TGG	+	chr8_2:44585597-44585616	Msa1249640:intron	40.0%
!!	GTATCCTTAACTTGCTTCTG+AGG	-	chr8_2:44585639-44585658	None:intergenic	40.0%
	AAGCAAGTTAAGGATACTGC+AGG	+	chr8_2:44585642-44585661	Msa1249640:five_prime_UTR	40.0%
!!!	GGTTCTCACCATTTTAACTC+TGG	+	chr8_2:44585924-44585943	Msa1249640:five_prime_UTR	40.0%
	GTCTGCTTCCAGAGTTAAAA+TGG	-	chr8_2:44585935-44585954	None:intergenic	40.0%
	TATTGGGCATGATTAGTAGG+CGG	-	chr8_2:44586065-44586084	None:intergenic	40.0%
	CATATGAGCCCATATAAGGT+GGG	-	chr8_2:44586107-44586126	None:intergenic	40.0%
	ATGGGCTCATATGGATATCA+TGG	+	chr8_2:44586114-44586133	Msa1249640:CDS	40.0%
!	GAAGATATCGGTTGCACAAA+CGG	-	chr8_2:44586272-44586291	None:intergenic	40.0%
	TCACTGCAAAAGACAAGTTG+TGG	-	chr8_2:44586452-44586471	None:intergenic	40.0%
	CACATACTTGAAGAGGAACA+TGG	+	chr8_2:44586490-44586509	Msa1249640:CDS	40.0%
	AAGAGGAACATGGATGATCA+GGG	+	chr8_2:44586500-44586519	Msa1249640:CDS	40.0%
	CAACAACGATCTTCAAATCC+AGG	-	chr8_2:44586730-44586749	None:intergenic	40.0%
!!!	TCAGCAATTTTGGGACGAAA+GGG	-	chr8_2:44586879-44586898	None:intergenic	40.0%
!	CTCTTTTGACCCCTAACATA+AGG	-	chr8_2:44586979-44586998	None:intergenic	40.0%
	GAGCATATTTCCCTAAACAG+GGG	+	chr8_2:44586996-44587015	Msa1249640:intron	40.0%
	ACAAAAGTGACCCCTGTTTA+GGG	-	chr8_2:44587009-44587028	None:intergenic	40.0%
	TTCATCTGTTGTTGAAGTGC+AGG	+	chr8_2:44587552-44587571	Msa1249640:CDS	40.0%
	AGGGTGATAAAATAAGGAGG+CGG	+	chr8_2:44587706-44587725	Msa1249640:intron	40.0%
	TGGAGAATATTGTTCGGATG+AGG	+	chr8_2:44587896-44587915	Msa1249640:three_prime_UTR	40.0%
	AACCCTGATTCCCAATCCAA+TGG	+	chr8_2:44584960-44584979	Msa1249640:five_prime_UTR	45.0%
	GAAGACATCGACAACACTTC+CGG	+	chr8_2:44585126-44585145	Msa1249640:five_prime_UTR	45.0%
	TTGGAGGGTTTACTCCAAAC+AGG	-	chr8_2:44585162-44585181	None:intergenic	45.0%
!!	TTGGCGGATTCAGATAAAGC+AGG	-	chr8_2:44585222-44585241	None:intergenic	45.0%
	GAAGGTTTCACCAATTCCTG+TGG	-	chr8_2:44585282-44585301	None:intergenic	45.0%
	GGAATTGGTGAAACCTTCCT+TGG	+	chr8_2:44585284-44585303	Msa1249640:five_prime_UTR	45.0%
	AGGCAAGGTCGATAAATCCA+AGG	-	chr8_2:44585304-44585323	None:intergenic	45.0%
	AAATACCTGCGATTGAGGCA+AGG	-	chr8_2:44585319-44585338	None:intergenic	45.0%
	CTCTCCTCAGAAGCAAGTTA+AGG	+	chr8_2:44585632-44585651	Msa1249640:five_prime_UTR	45.0%
	TCAAACACGTCTTCCAATGG+AGG	+	chr8_2:44585720-44585739	Msa1249640:five_prime_UTR	45.0%
	ATGGAACATCTACCAAGAGC+TGG	+	chr8_2:44585822-44585841	Msa1249640:five_prime_UTR	45.0%
	TCCGGTTCCTGGATGAATTT+CGG	-	chr8_2:44585875-44585894	None:intergenic	45.0%
	AATTCATCCAGGAACCGGAA+TGG	+	chr8_2:44585876-44585895	Msa1249640:five_prime_UTR	45.0%
	CACCCAATCCCACCTTATAT+GGG	+	chr8_2:44586096-44586115	Msa1249640:CDS	45.0%
	AGCCCATATAAGGTGGGATT+GGG	-	chr8_2:44586101-44586120	None:intergenic	45.0%
	CCATATGAGCCCATATAAGG+TGG	-	chr8_2:44586108-44586127	None:intergenic	45.0%
	CCACCTTATATGGGCTCATA+TGG	+	chr8_2:44586105-44586124	Msa1249640:CDS	45.0%
	TGCACAAACGGTACACTTCT+CGG	-	chr8_2:44586260-44586279	None:intergenic	45.0%
	GCAACGACACATACTTGAAG+AGG	+	chr8_2:44586483-44586502	Msa1249640:CDS	45.0%
	GAAGAGGAACATGGATGATC+AGG	+	chr8_2:44586499-44586518	Msa1249640:CDS	45.0%
!!!	CAGCAATTTTGGGACGAAAG+GGG	-	chr8_2:44586878-44586897	None:intergenic	45.0%
!!!	CTCAGCAATTTTGGGACGAA+AGG	-	chr8_2:44586880-44586899	None:intergenic	45.0%
!	GGCGTCTTCTCAGCAATTTT+GGG	-	chr8_2:44586888-44586907	None:intergenic	45.0%
!	TGGCGTCTTCTCAGCAATTT+TGG	-	chr8_2:44586889-44586908	None:intergenic	45.0%
!!!	GCGACTATGACCCCTTTTTT+GGG	-	chr8_2:44586917-44586936	None:intergenic	45.0%
!!!	TGCGACTATGACCCCTTTTT+TGG	-	chr8_2:44586918-44586937	None:intergenic	45.0%
	GACAAAAGTGACCCCTGTTT+AGG	-	chr8_2:44587010-44587029	None:intergenic	45.0%
	GAATATTGTTCGGATGAGGC+AGG	+	chr8_2:44587900-44587919	Msa1249640:three_prime_UTR	45.0%
!!!	AATTGTAATTTTTTTAAATA+GGG	-	chr8_2:44586851-44586870	None:intergenic	5.0%
!!!	AAATTGTAATTTTTTTAAAT+AGG	-	chr8_2:44586852-44586871	None:intergenic	5.0%
!!!	ATTTAAAAAAATTACAATTT+TGG	+	chr8_2:44586852-44586871	Msa1249640:intron	5.0%
!	GGCCATTGGATTGGGAATCA+GGG	-	chr8_2:44584965-44584984	None:intergenic	50.0%
!	TGGCCATTGGATTGGGAATC+AGG	-	chr8_2:44584966-44584985	None:intergenic	50.0%
	GCGTTCATGGCCATTGGATT+GGG	-	chr8_2:44584973-44584992	None:intergenic	50.0%
	AGCGTTCATGGCCATTGGAT+TGG	-	chr8_2:44584974-44584993	None:intergenic	50.0%
!	AACAATCTGGCTCCAAGGTG+CGG	-	chr8_2:44585010-44585029	None:intergenic	50.0%
	TGACGGCGATGAATGAGGTA+CGG	-	chr8_2:44585052-44585071	None:intergenic	50.0%
	CCAAACAGGTCTCTTACCAC+CGG	-	chr8_2:44585148-44585167	None:intergenic	50.0%
!	GAAGAAGCAGCAGCATTGGA+GGG	-	chr8_2:44585177-44585196	None:intergenic	50.0%
!	CTCCAATGCTGCTGCTTCTT+CGG	+	chr8_2:44585176-44585195	Msa1249640:five_prime_UTR	50.0%
	ATCTGAATCCGCCAAAGCTC+CGG	+	chr8_2:44585227-44585246	Msa1249640:five_prime_UTR	50.0%
!!	AATTCCTGTGGTGGTGATGG+CGG	-	chr8_2:44585270-44585289	None:intergenic	50.0%
!	ACCAATTCCTGTGGTGGTGA+TGG	-	chr8_2:44585273-44585292	None:intergenic	50.0%
	GGTTTCACCAATTCCTGTGG+TGG	-	chr8_2:44585279-44585298	None:intergenic	50.0%
	TATGATTGTGGGACCCTGGT+GGG	-	chr8_2:44585791-44585810	None:intergenic	50.0%
	TTATGATTGTGGGACCCTGG+TGG	-	chr8_2:44585792-44585811	None:intergenic	50.0%
	GAGTTATGATTGTGGGACCC+TGG	-	chr8_2:44585795-44585814	None:intergenic	50.0%
	TTGGGCACAAATCCAGCTCT+TGG	-	chr8_2:44585837-44585856	None:intergenic	50.0%
	GCCGAAATTCATCCAGGAAC+CGG	+	chr8_2:44585871-44585890	Msa1249640:five_prime_UTR	50.0%
	CATTCCAGTCCTGATTTCCG+CGG	-	chr8_2:44585968-44585987	None:intergenic	50.0%
	TATAAGGTGGGATTGGGTGG+CGG	-	chr8_2:44586095-44586114	None:intergenic	50.0%
	CCATATAAGGTGGGATTGGG+TGG	-	chr8_2:44586098-44586117	None:intergenic	50.0%
	CCACCCAATCCCACCTTATA+TGG	+	chr8_2:44586095-44586114	Msa1249640:CDS	50.0%
	GAGCCCATATAAGGTGGGAT+TGG	-	chr8_2:44586102-44586121	None:intergenic	50.0%
	GTATGGTCCACCTTTGCATG+TGG	+	chr8_2:44586229-44586248	Msa1249640:CDS	50.0%
	ACAAACGGTACACTTCTCGG+AGG	-	chr8_2:44586257-44586276	None:intergenic	50.0%
	GTATGCAACTCAGAATCCGG+AGG	-	chr8_2:44586311-44586330	None:intergenic	50.0%
	GAACATGGATGATCAGGGCT+GGG	+	chr8_2:44586505-44586524	Msa1249640:CDS	50.0%
	ACGCCACGTGTCCCAAAAAA+GGG	+	chr8_2:44586903-44586922	Msa1249640:intron	50.0%
!!	GAGGAAGCGTTCATGGCCAT+TGG	-	chr8_2:44584979-44584998	None:intergenic	55.0%
!	GAGGCAACAATCTGGCTCCA+AGG	-	chr8_2:44585015-44585034	None:intergenic	55.0%
	GACATCGACAACACTTCCGG+TGG	+	chr8_2:44585129-44585148	Msa1249640:five_prime_UTR	55.0%
	CCGGTGGTAAGAGACCTGTT+TGG	+	chr8_2:44585145-44585164	Msa1249640:five_prime_UTR	55.0%
!	CGAAGAAGCAGCAGCATTGG+AGG	-	chr8_2:44585178-44585197	None:intergenic	55.0%
	GACCGAAGAAGCAGCAGCAT+TGG	-	chr8_2:44585181-44585200	None:intergenic	55.0%
!	TGCTGCTGCTTCTTCGGTCA+TGG	+	chr8_2:44585182-44585201	Msa1249640:five_prime_UTR	55.0%
!!	CGGTCATGGATGCTGACTCA+TGG	+	chr8_2:44585196-44585215	Msa1249640:five_prime_UTR	55.0%
	GCCATCACCACCACAGGAAT+TGG	+	chr8_2:44585269-44585288	Msa1249640:five_prime_UTR	55.0%
	ATCGACCTTGCCTCAATCGC+AGG	+	chr8_2:44585311-44585330	Msa1249640:five_prime_UTR	55.0%
	TGAGGCGCAGACATTTGTGG+CGG	-	chr8_2:44585753-44585772	None:intergenic	55.0%
	CCTTGAGGCGCAGACATTTG+TGG	-	chr8_2:44585756-44585775	None:intergenic	55.0%
	CCACAAATGTCTGCGCCTCA+AGG	+	chr8_2:44585753-44585772	Msa1249640:five_prime_UTR	55.0%
	GGGACAGCAACGAAACCTTG+AGG	-	chr8_2:44585771-44585790	None:intergenic	55.0%
	GTTTCGTTGCTGTCCCACCA+GGG	+	chr8_2:44585775-44585794	Msa1249640:five_prime_UTR	55.0%
	CTGGATGAATTTCGGCGCTG+AGG	-	chr8_2:44585867-44585886	None:intergenic	55.0%
!	CTCAGCGCCGAAATTCATCC+AGG	+	chr8_2:44585865-44585884	Msa1249640:five_prime_UTR	55.0%
	TCATCCAGGAACCGGAATGG+CGG	+	chr8_2:44585879-44585898	Msa1249640:five_prime_UTR	55.0%
	GTTGTAGACCACCGCCATTC+CGG	-	chr8_2:44585893-44585912	None:intergenic	55.0%
!	CTCTGGAAGCAGACGTGATC+AGG	+	chr8_2:44585941-44585960	Msa1249640:five_prime_UTR	55.0%
	TGATCAGGACCGCGGAAATC+AGG	+	chr8_2:44585956-44585975	Msa1249640:five_prime_UTR	55.0%
	GGTGGAGGCCTTACAAACTG+TGG	-	chr8_2:44586044-44586063	None:intergenic	55.0%
	GCATGATTAGTAGGCGGTGG+AGG	-	chr8_2:44586059-44586078	None:intergenic	55.0%
	TGGGCATGATTAGTAGGCGG+TGG	-	chr8_2:44586062-44586081	None:intergenic	55.0%
	ATAAGGTGGGATTGGGTGGC+GGG	-	chr8_2:44586094-44586113	None:intergenic	55.0%
	TGCAACTCAGAATCCGGAGG+TGG	-	chr8_2:44586308-44586327	None:intergenic	55.0%
	GGAACATGGATGATCAGGGC+TGG	+	chr8_2:44586504-44586523	Msa1249640:CDS	55.0%
	GACGCCACGTGTCCCAAAAA+AGG	+	chr8_2:44586902-44586921	Msa1249640:intron	55.0%
	CGCCACGTGTCCCAAAAAAG+GGG	+	chr8_2:44586904-44586923	Msa1249640:intron	55.0%
!!!	GACCCCTTTTTTGGGACACG+TGG	-	chr8_2:44586909-44586928	None:intergenic	55.0%
	TGTTCGGATGAGGCAGGAAG+CGG	+	chr8_2:44587906-44587925	Msa1249640:three_prime_UTR	55.0%
!	AATCTGGCTCCAAGGTGCGG+CGG	-	chr8_2:44585007-44585026	None:intergenic	60.0%
	CAGCGGTGGAGGCAACAATC+TGG	-	chr8_2:44585023-44585042	None:intergenic	60.0%
	ATGAGGTACGGCGGAAGCAG+CGG	-	chr8_2:44585040-44585059	None:intergenic	60.0%
	CGGCGATGAATGAGGTACGG+CGG	-	chr8_2:44585049-44585068	None:intergenic	60.0%
!	GGAGGTGACGGCGATGAATG+AGG	-	chr8_2:44585057-44585076	None:intergenic	60.0%
	GAGGAAGGAGGAGGAGGTGA+CGG	-	chr8_2:44585069-44585088	None:intergenic	60.0%
!!	GACGGCGAAAGTGTCGACAG+CGG	-	chr8_2:44585094-44585113	None:intergenic	60.0%
	CGACACTTTCGCCGTCACTG+AGG	+	chr8_2:44585098-44585117	Msa1249640:five_prime_UTR	60.0%
	CACTTTCGCCGTCACTGAGG+CGG	+	chr8_2:44585101-44585120	Msa1249640:five_prime_UTR	60.0%
!	CGATTTCGCCGGAGCTTTGG+CGG	-	chr8_2:44585238-44585257	None:intergenic	60.0%
	TGGCGATTTCGCCGGAGCTT+TGG	-	chr8_2:44585241-44585260	None:intergenic	60.0%
	CAGACATTTGTGGCGGCTGG+CGG	-	chr8_2:44585746-44585765	None:intergenic	60.0%
	GGTTTCGTTGCTGTCCCACC+AGG	+	chr8_2:44585774-44585793	Msa1249640:five_prime_UTR	60.0%
!	CGGCGCTGAGGCTGATCATT+GGG	-	chr8_2:44585855-44585874	None:intergenic	60.0%
!	TCGGCGCTGAGGCTGATCAT+TGG	-	chr8_2:44585856-44585875	None:intergenic	60.0%
	GGTCTACAACCACGCGGAGA+TGG	+	chr8_2:44585903-44585922	Msa1249640:five_prime_UTR	60.0%
	TGGTGAGAACCATCTCCGCG+TGG	-	chr8_2:44585915-44585934	None:intergenic	60.0%
	AGCAGACGTGATCAGGACCG+CGG	+	chr8_2:44585948-44585967	Msa1249640:five_prime_UTR	60.0%
	AGGACCGCGGAAATCAGGAC+TGG	+	chr8_2:44585961-44585980	Msa1249640:five_prime_UTR	60.0%
!!	GGGTGGCGGGGGATAGTATT+GGG	-	chr8_2:44586081-44586100	None:intergenic	60.0%
!!	TGGGTGGCGGGGGATAGTAT+TGG	-	chr8_2:44586082-44586101	None:intergenic	60.0%
	TAAGGTGGGATTGGGTGGCG+GGG	-	chr8_2:44586093-44586112	None:intergenic	60.0%
	GGAGGCTCCACATGCAAAGG+TGG	-	chr8_2:44586239-44586258	None:intergenic	60.0%
	CTCGGAGGCTCCACATGCAA+AGG	-	chr8_2:44586242-44586261	None:intergenic	60.0%
	GTTCGGATGAGGCAGGAAGC+GGG	+	chr8_2:44587907-44587926	Msa1249640:three_prime_UTR	60.0%
!!	GGCGGGAGAGGAAGCGTTCA+TGG	-	chr8_2:44584986-44585005	None:intergenic	65.0%
!!	GAAAGTGTCGACAGCGGCGG+AGG	-	chr8_2:44585088-44585107	None:intergenic	65.0%
!!	GGCGAAAGTGTCGACAGCGG+CGG	-	chr8_2:44585091-44585110	None:intergenic	65.0%
	TTTCGCCGTCACTGAGGCGG+CGG	+	chr8_2:44585104-44585123	Msa1249640:five_prime_UTR	65.0%
	GTCTTCCGCCGCCTCAGTGA+CGG	-	chr8_2:44585112-44585131	None:intergenic	65.0%
!!	GGTGGTGGTGGCGATTTCGC+CGG	-	chr8_2:44585249-44585268	None:intergenic	65.0%
!!	TGTGGTGGTGATGGCGGTGG+TGG	-	chr8_2:44585264-44585283	None:intergenic	65.0%
	ACCACCGCCATCACCACCAC+AGG	+	chr8_2:44585263-44585282	Msa1249640:five_prime_UTR	65.0%
!!	TCCTGTGGTGGTGATGGCGG+TGG	-	chr8_2:44585267-44585286	None:intergenic	65.0%
	GCGCAGACATTTGTGGCGGC+TGG	-	chr8_2:44585749-44585768	None:intergenic	65.0%
	TCCAGGAACCGGAATGGCGG+TGG	+	chr8_2:44585882-44585901	Msa1249640:five_prime_UTR	65.0%
	ACCACCGCCATTCCGGTTCC+TGG	-	chr8_2:44585886-44585905	None:intergenic	65.0%
	GGCGGTGGTCTACAACCACG+CGG	+	chr8_2:44585897-44585916	Msa1249640:five_prime_UTR	65.0%
!	AAGGTGGGATTGGGTGGCGG+GGG	-	chr8_2:44586092-44586111	None:intergenic	65.0%
	TACGGCGGAAGCAGCGGTGG+AGG	-	chr8_2:44585034-44585053	None:intergenic	70.0%
	AGGTACGGCGGAAGCAGCGG+TGG	-	chr8_2:44585037-44585056	None:intergenic	70.0%
	ACAGCGGCGGAGGAAGGAGG+AGG	-	chr8_2:44585078-44585097	None:intergenic	70.0%
	TCGACAGCGGCGGAGGAAGG+AGG	-	chr8_2:44585081-44585100	None:intergenic	70.0%
!	GTGTCGACAGCGGCGGAGGA+AGG	-	chr8_2:44585084-44585103	None:intergenic	70.0%
!!	GGTGGTGATGGCGGTGGTGG+TGG	-	chr8_2:44585261-44585280	None:intergenic	70.0%
	TGGCTCCAAGGTGCGGCGGC+GGG	-	chr8_2:44585003-44585022	None:intergenic	75.0%
	CTGGCTCCAAGGTGCGGCGG+CGG	-	chr8_2:44585004-44585023	None:intergenic	75.0%
	GCGGCGGAGGAAGGAGGAGG+AGG	-	chr8_2:44585075-44585094	None:intergenic	75.0%
	TGGCGGCTGGCGGCCTCCAT+TGG	-	chr8_2:44585736-44585755	None:intergenic	75.0%
!	CCAAGGTGCGGCGGCGGGAG+AGG	-	chr8_2:44584998-44585017	None:intergenic	80.0%
!	CCTCTCCCGCCGCCGCACCT+TGG	+	chr8_2:44584995-44585014	Msa1249640:five_prime_UTR	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_2	gene	44584889	44588278	44584889	ID=Msa1249640;Name=Msa1249640
chr8_2	mRNA	44584889	44588278	44584889	ID=Msa1249640-mRNA-1;Parent=Msa1249640;Name=Msa1249640-mRNA-1;_AED=0.08;_eAED=0.08;_QI=907\|1\|1\|1\|0.66\|0.57\|7\|262\|179
chr8_2	exon	44584889	44585332	44584889	ID=Msa1249640-mRNA-1:exon:13477;Parent=Msa1249640-mRNA-1
chr8_2	exon	44585612	44586135	44585612	ID=Msa1249640-mRNA-1:exon:13478;Parent=Msa1249640-mRNA-1
chr8_2	exon	44586210	44586363	44586210	ID=Msa1249640-mRNA-1:exon:13479;Parent=Msa1249640-mRNA-1
chr8_2	exon	44586469	44586559	44586469	ID=Msa1249640-mRNA-1:exon:13480;Parent=Msa1249640-mRNA-1
chr8_2	exon	44587502	44587573	44587502	ID=Msa1249640-mRNA-1:exon:13481;Parent=Msa1249640-mRNA-1
chr8_2	exon	44587708	44588105	44587708	ID=Msa1249640-mRNA-1:exon:13482;Parent=Msa1249640-mRNA-1
chr8_2	exon	44588253	44588278	44588253	ID=Msa1249640-mRNA-1:exon:13483;Parent=Msa1249640-mRNA-1
chr8_2	five_prime_UTR	44584889	44585332	44584889	ID=Msa1249640-mRNA-1:five_prime_utr;Parent=Msa1249640-mRNA-1
chr8_2	five_prime_UTR	44585612	44586074	44585612	ID=Msa1249640-mRNA-1:five_prime_utr;Parent=Msa1249640-mRNA-1
chr8_2	CDS	44586075	44586135	44586075	ID=Msa1249640-mRNA-1:cds;Parent=Msa1249640-mRNA-1
chr8_2	CDS	44586210	44586363	44586210	ID=Msa1249640-mRNA-1:cds;Parent=Msa1249640-mRNA-1
chr8_2	CDS	44586469	44586559	44586469	ID=Msa1249640-mRNA-1:cds;Parent=Msa1249640-mRNA-1
chr8_2	CDS	44587502	44587573	44587502	ID=Msa1249640-mRNA-1:cds;Parent=Msa1249640-mRNA-1
chr8_2	CDS	44587708	44587869	44587708	ID=Msa1249640-mRNA-1:cds;Parent=Msa1249640-mRNA-1
chr8_2	three_prime_UTR	44587870	44588105	44587870	ID=Msa1249640-mRNA-1:three_prime_utr;Parent=Msa1249640-mRNA-1
chr8_2	three_prime_UTR	44588253	44588278	44588253	ID=Msa1249640-mRNA-1:three_prime_utr;Parent=Msa1249640-mRNA-1

Gene Sequence

>Msa1249640

ATGCCCAATACTATCCCCCGCCACCCAATCCCACCTTATATGGGCTCATATGGATATCATGACCTAACGCTTCAAATGATGTATGGTCCACCTTTGCATGTGGAGCCTCCGAGAAGTGTACCGTTTGTGCAACCGATATCTTCTGCAATATTTTTTCCACCTCCGGATTCTGAGTTGCATACTAAGATTGTCAACCAGATTGACTACTATTTCAGTGATCTCAATTTAAGCAACGACACATACTTGAAGAGGAACATGGATGATCAGGGCTGGGTTCCTCTAAATTTAATAGCAGGATTCAATAAAGTCAAACTTTTGACTGACAACATTCAGATTGTATTGGATGCTGTTCGTACTTCATCTGTTGTTGAAGTGCAGGGTGATAAAATAAGGAGGCGGAATTGGATCACACTTTCTTCACAGTTTCATAACGTCAATGGTTCTCAAGTTGTTGCACAACTAGCAAAGAATATCCAAAACATTGATCTGGAAACAAATAAAAATGGTGCATCTGGAGAACTGCATGTTTCAAACAGTTAG

Protein sequence

>Msa1249640

MPNTIPRHPIPPYMGSYGYHDLTLQMMYGPPLHVEPPRSVPFVQPISSAIFFPPPDSELHTKIVNQIDYYFSDLNLSNDTYLKRNMDDQGWVPLNLIAGFNKVKLLTDNIQIVLDAVRTSSVVEVQGDKIRRRNWITLSSQFHNVNGSQVVAQLAKNIQNIDLETNKNGASGELHVSNS*