AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067588


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067588.t1 MTR_4g083370 87.151 179 14 1 1 179 1 170 1.36e-96 280
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067588.t1 AT4G26370 61.404 114 41 1 66 179 69 179 3.70e-38 131
MS.gene067588.t1 AT4G26370 61.404 114 41 1 66 179 69 179 3.70e-38 131
MS.gene067588.t1 AT4G26370 60.526 114 42 1 66 179 69 179 3.46e-37 130

Find 48 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGGTTTGGGAAGGAATT+TGG 0.119074 4.1:+24400978 None:intergenic
TGAAGTAGCGGTGTTAATTT+TGG 0.202201 4.1:+24400960 None:intergenic
TTGGGTTTGGGAAGGAATTT+GGG 0.203019 4.1:+24400979 None:intergenic
TCATCTGTTTCAACGATAAC+AGG 0.318092 4.1:+24400478 None:intergenic
GCAAAAGCGGTGGTTGAGTT+TGG 0.331587 4.1:+24401044 None:intergenic
TATAATTTATGCTTCGTGTT+TGG 0.348060 4.1:-24400695 MS.gene067588:CDS
TGCTTCGTGTTTGGAGGGAT+TGG 0.362504 4.1:-24400686 MS.gene067588:CDS
AAGAAGCTAATGAACTTCTA+AGG 0.369066 4.1:-24400456 MS.gene067588:CDS
AGAGAGCATTTCAGAGATAA+AGG 0.376806 4.1:+24400934 None:intergenic
GAGTATAATCACATGAGTTT+CGG 0.405577 4.1:-24400508 MS.gene067588:CDS
ATTTAACAAGGACAAATTGT+TGG 0.439004 4.1:-24400530 MS.gene067588:CDS
TGGAGGGATTGGACCCGGTT+CGG 0.439014 4.1:-24400675 MS.gene067588:CDS
AATTTATGCTTCGTGTTTGG+AGG 0.449542 4.1:-24400692 MS.gene067588:CDS
AGAAGTTGAAGGTGAAGAGA+GGG 0.454774 4.1:+24401071 None:intergenic
CGTTTCTCAAATAACCGAAC+CGG 0.455058 4.1:+24400661 None:intergenic
GTCTTTGTCGAGTGCTAAAG+TGG 0.463142 4.1:+24400903 None:intergenic
AGGTGAAGAGAGGGGAAGTA+AGG 0.465052 4.1:+24401080 None:intergenic
AAGAAGTTGAAGGTGAAGAG+AGG 0.465590 4.1:+24401070 None:intergenic
ATTCAACTCGAGTCTGCAAT+TGG 0.472202 4.1:-24400430 MS.gene067588:CDS
TGGTGGTTTAGTAGTGAAGA+GGG 0.487919 4.1:+24401018 None:intergenic
GTGTTTGGAGGGATTGGACC+CGG 0.500612 4.1:-24400680 MS.gene067588:CDS
TTGCCTAAACTCGATAAAAC+CGG 0.503153 4.1:-24400838 MS.gene067588:CDS
CGGTTCGGTTATTTGAGAAA+CGG 0.523373 4.1:-24400660 MS.gene067588:CDS
ATTTATGCTTCGTGTTTGGA+GGG 0.526272 4.1:-24400691 MS.gene067588:CDS
GCAAGTTCTCTAGCGGCTCT+CGG 0.536127 4.1:+24400802 None:intergenic
AGTTTGGAAGAAGAAGTTGA+AGG 0.542605 4.1:+24401060 None:intergenic
GAAGTTGAAGGTGAAGAGAG+GGG 0.577370 4.1:+24401072 None:intergenic
TATAATCACATGAGTTTCGG+AGG 0.587444 4.1:-24400505 MS.gene067588:CDS
GGAAGTGAGAGTAACGGTGG+TGG 0.588538 4.1:+24401001 None:intergenic
CTCCACACTTCAACAATGGA+GGG 0.590137 4.1:-24401105 None:intergenic
TGGGTTTGGGAAGGAATTTG+GGG 0.610576 4.1:+24400980 None:intergenic
GTGGTGGTTTAGTAGTGAAG+AGG 0.622444 4.1:+24401017 None:intergenic
GTGAAGAGGGGAAGCAAAAG+CGG 0.626097 4.1:+24401031 None:intergenic
GGTGGTTTAGTAGTGAAGAG+GGG 0.629211 4.1:+24401019 None:intergenic
TGGGGAAGTGAGAGTAACGG+TGG 0.638686 4.1:+24400998 None:intergenic
ATTTGGGGAAGTGAGAGTAA+CGG 0.641749 4.1:+24400995 None:intergenic
GGTTGGTTATGAATTTAACA+AGG 0.642550 4.1:-24400542 MS.gene067588:CDS
TCTGTTTCAACGATAACAGG+TGG 0.649999 4.1:+24400481 None:intergenic
AGATAAAGGTGATGAAGTAG+CGG 0.660310 4.1:+24400948 None:intergenic
TCTCGGACTACAAAACCGAC+CGG 0.664343 4.1:+24400819 None:intergenic
ATAATCACATGAGTTTCGGA+GGG 0.671556 4.1:-24400504 MS.gene067588:CDS
TTACAGAGCAAGTTCTCTAG+CGG 0.672676 4.1:+24400795 None:intergenic
GTTTCTCAAATAACCGAACC+GGG 0.676933 4.1:+24400662 None:intergenic
TCTCTGAAATGCTCTCTCCG+TGG 0.679129 4.1:-24400928 MS.gene067588:CDS
AAACGGATGAATGAGCGTAG+AGG 0.681961 4.1:-24400643 MS.gene067588:intron
CTAAACTCGATAAAACCGGT+CGG 0.694768 4.1:-24400834 MS.gene067588:CDS
CGAGTGCTAAAGTGGAACCA+CGG 0.698369 4.1:+24400911 None:intergenic
AAGAGGGGAAGCAAAAGCGG+TGG 0.698794 4.1:+24401034 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTGTTTGTTTTTTATAG+AGG - chr4.1:24400944-24400963 MS.gene067588:CDS 20.0%
! ATTTAACAAGGACAAATTGT+TGG - chr4.1:24400977-24400996 MS.gene067588:CDS 25.0%
! TATAATTTATGCTTCGTGTT+TGG - chr4.1:24400812-24400831 MS.gene067588:CDS 25.0%
!! GTATTTGGAATTAACGTTAA+CGG - chr4.1:24400771-24400790 MS.gene067588:intron 25.0%
!!! ATTGTTAACTGTTTTGGTTT+TGG - chr4.1:24400889-24400908 MS.gene067588:CDS 25.0%
!!! TGTTTGTTTTTTATAGAGGT+TGG - chr4.1:24400948-24400967 MS.gene067588:CDS 25.0%
AAGAAGCTAATGAACTTCTA+AGG - chr4.1:24401051-24401070 MS.gene067588:CDS 30.0%
GAGTATAATCACATGAGTTT+CGG - chr4.1:24400999-24401018 MS.gene067588:CDS 30.0%
! GAGGTAATTGTTAACTGTTT+TGG - chr4.1:24400883-24400902 MS.gene067588:CDS 30.0%
! GGTTGGTTATGAATTTAACA+AGG - chr4.1:24400965-24400984 MS.gene067588:CDS 30.0%
!! GTGTACTGCATTTTAGTATT+TGG - chr4.1:24400756-24400775 MS.gene067588:intron 30.0%
AATTTATGCTTCGTGTTTGG+AGG - chr4.1:24400815-24400834 MS.gene067588:CDS 35.0%
AGATAAAGGTGATGAAGTAG+CGG + chr4.1:24400562-24400581 None:intergenic 35.0%
AGTTTGGAAGAAGAAGTTGA+AGG + chr4.1:24400450-24400469 None:intergenic 35.0%
ATAATCACATGAGTTTCGGA+GGG - chr4.1:24401003-24401022 MS.gene067588:CDS 35.0%
ATTTATGCTTCGTGTTTGGA+GGG - chr4.1:24400816-24400835 MS.gene067588:CDS 35.0%
TATAATCACATGAGTTTCGG+AGG - chr4.1:24401002-24401021 MS.gene067588:CDS 35.0%
TCATCTGTTTCAACGATAAC+AGG + chr4.1:24401032-24401051 None:intergenic 35.0%
TTGCCTAAACTCGATAAAAC+CGG - chr4.1:24400669-24400688 MS.gene067588:CDS 35.0%
! TTTGGTTGTTGTTGGTATTG+TGG - chr4.1:24400907-24400926 MS.gene067588:CDS 35.0%
!! AGAGAGCATTTCAGAGATAA+AGG + chr4.1:24400576-24400595 None:intergenic 35.0%
!! GAAGTAGCGGTGTTAATTTT+GGG + chr4.1:24400549-24400568 None:intergenic 35.0%
!! TGAAGTAGCGGTGTTAATTT+TGG + chr4.1:24400550-24400569 None:intergenic 35.0%
!! TTAACGGTCTTTGATTTTGC+AGG - chr4.1:24400787-24400806 MS.gene067588:intron 35.0%
!!! GTTTTGGTTTTGGTTGTTGT+TGG - chr4.1:24400899-24400918 MS.gene067588:CDS 35.0%
!!! TGTTAATTTTGGGTTTGGGA+AGG + chr4.1:24400539-24400558 None:intergenic 35.0%
AAGAAGTTGAAGGTGAAGAG+AGG + chr4.1:24400440-24400459 None:intergenic 40.0%
AGAAGTTGAAGGTGAAGAGA+GGG + chr4.1:24400439-24400458 None:intergenic 40.0%
ATTCAACTCGAGTCTGCAAT+TGG - chr4.1:24401077-24401096 MS.gene067588:CDS 40.0%
ATTTGGGGAAGTGAGAGTAA+CGG + chr4.1:24400515-24400534 None:intergenic 40.0%
CGTTTCTCAAATAACCGAAC+CGG + chr4.1:24400849-24400868 None:intergenic 40.0%
CTAAACTCGATAAAACCGGT+CGG - chr4.1:24400673-24400692 MS.gene067588:CDS 40.0%
GTTTCTCAAATAACCGAACC+GGG + chr4.1:24400848-24400867 None:intergenic 40.0%
TCTGTTTCAACGATAACAGG+TGG + chr4.1:24401029-24401048 None:intergenic 40.0%
TGGTGGTTTAGTAGTGAAGA+GGG + chr4.1:24400492-24400511 None:intergenic 40.0%
TTACAGAGCAAGTTCTCTAG+CGG + chr4.1:24400715-24400734 None:intergenic 40.0%
TTGGGTTTGGGAAGGAATTT+GGG + chr4.1:24400531-24400550 None:intergenic 40.0%
! TTTGGGTTTGGGAAGGAATT+TGG + chr4.1:24400532-24400551 None:intergenic 40.0%
!! CGGTTCGGTTATTTGAGAAA+CGG - chr4.1:24400847-24400866 MS.gene067588:CDS 40.0%
!!! AGCGGTGTTAATTTTGGGTT+TGG + chr4.1:24400544-24400563 None:intergenic 40.0%
!!! GCGGTGTTAATTTTGGGTTT+GGG + chr4.1:24400543-24400562 None:intergenic 40.0%
AAACGGATGAATGAGCGTAG+AGG - chr4.1:24400864-24400883 MS.gene067588:CDS 45.0%
GAAGTTGAAGGTGAAGAGAG+GGG + chr4.1:24400438-24400457 None:intergenic 45.0%
GGTGGTTTAGTAGTGAAGAG+GGG + chr4.1:24400491-24400510 None:intergenic 45.0%
GTCTTTGTCGAGTGCTAAAG+TGG + chr4.1:24400607-24400626 None:intergenic 45.0%
GTGGTGGTTTAGTAGTGAAG+AGG + chr4.1:24400493-24400512 None:intergenic 45.0%
TGGGTTTGGGAAGGAATTTG+GGG + chr4.1:24400530-24400549 None:intergenic 45.0%
!! CGACCGGTTTTATCGAGTTT+AGG + chr4.1:24400675-24400694 None:intergenic 45.0%
AGGTGAAGAGAGGGGAAGTA+AGG + chr4.1:24400430-24400449 None:intergenic 50.0%
CGAGTGCTAAAGTGGAACCA+CGG + chr4.1:24400599-24400618 None:intergenic 50.0%
GCAAAAGCGGTGGTTGAGTT+TGG + chr4.1:24400466-24400485 None:intergenic 50.0%
GTGAAGAGGGGAAGCAAAAG+CGG + chr4.1:24400479-24400498 None:intergenic 50.0%
TCTCGGACTACAAAACCGAC+CGG + chr4.1:24400691-24400710 None:intergenic 50.0%
TGCTTCGTGTTTGGAGGGAT+TGG - chr4.1:24400821-24400840 MS.gene067588:CDS 50.0%
!! TCTCTGAAATGCTCTCTCCG+TGG - chr4.1:24400579-24400598 MS.gene067588:intron 50.0%
AAGAGGGGAAGCAAAAGCGG+TGG + chr4.1:24400476-24400495 None:intergenic 55.0%
GCAAGTTCTCTAGCGGCTCT+CGG + chr4.1:24400708-24400727 None:intergenic 55.0%
GGAAGTGAGAGTAACGGTGG+TGG + chr4.1:24400509-24400528 None:intergenic 55.0%
GTGTTTGGAGGGATTGGACC+CGG - chr4.1:24400827-24400846 MS.gene067588:CDS 55.0%
TGGGGAAGTGAGAGTAACGG+TGG + chr4.1:24400512-24400531 None:intergenic 55.0%
TGGAGGGATTGGACCCGGTT+CGG - chr4.1:24400832-24400851 MS.gene067588:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 24400417 24401112 24400417 ID=MS.gene067588
chr4.1 mRNA 24400417 24401112 24400417 ID=MS.gene067588.t1;Parent=MS.gene067588
chr4.1 exon 24400799 24401112 24400799 ID=MS.gene067588.t1.exon1;Parent=MS.gene067588.t1
chr4.1 CDS 24400799 24401112 24400799 ID=cds.MS.gene067588.t1;Parent=MS.gene067588.t1
chr4.1 exon 24400644 24400720 24400644 ID=MS.gene067588.t1.exon2;Parent=MS.gene067588.t1
chr4.1 CDS 24400644 24400720 24400644 ID=cds.MS.gene067588.t1;Parent=MS.gene067588.t1
chr4.1 exon 24400417 24400565 24400417 ID=MS.gene067588.t1.exon3;Parent=MS.gene067588.t1
chr4.1 CDS 24400417 24400565 24400417 ID=cds.MS.gene067588.t1;Parent=MS.gene067588.t1
Gene Sequence

>MS.gene067588

ATGGAGGGAGCCTTACTTCCCCTCTCTTCACCTTCAACTTCTTCTTCCAAACTCAACCACCGCTTTTGCTTCCCCTCTTCACTACTAAACCACCACCGTTACTCTCACTTCCCCAAATTCCTTCCCAAACCCAAAATTAACACCGCTACTTCATCACCTTTATCTCTGAAATGCTCTCTCCGTGGTTCCACTTTAGCACTCGACAAAGACGAAACTTCTGTCTCAGAAATAACTGATAACTCTTCAATTTCTTTGCCTAAACTCGATAAAACCGGTCGGTTTTGTAGTCCGAGAGCCGCTAGAGAACTTGCTCTGTAAGCACATTACAGTATTACTATTGTGTACTGCATTTTAGTATTTGGAATTAACGTTAACGGTCTTTGATTTTGCAGGTCTATAATTTATGCTTCGTGTTTGGAGGGATTGGACCCGGTTCGGTTATTTGAGAAACGGATGAATGAGCGTAGAGGTAATTGTTAACTGTTTTGGTTTTGGTTGTTGTTGGTATTGTGGTGAGAATTGAAGTTAATGTGTTTGTTTTTTATAGAGGTTGGTTATGAATTTAACAAGGACAAATTGTTGGAGTATAATCACATGAGTTTCGGAGGGCCACCTGTTATCGTTGAAACAGATGAAGAAGCTAATGAACTTCTAAGGAACATTCAACTCGAGTCTGCAATTGGTAACGATATATAA

Protein sequence

>MS.gene067588.t1

MEGALLPLSSPSTSSSKLNHRFCFPSSLLNHHRYSHFPKFLPKPKINTATSSPLSLKCSLRGSTLALDKDETSVSEITDNSSISLPKLDKTGRFCSPRAARELALSIIYASCLEGLDPVRLFEKRMNERREVGYEFNKDKLLEYNHMSFGGPPVIVETDEEANELLRNIQLESAIGNDI