Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071480.t1 | XP_003517200.1 | 100 | 85 | 0 | 0 | 1 | 85 | 1 | 85 | 2.20E-37 | 164.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071480.t1 | Q94JY4 | 90.6 | 85 | 8 | 0 | 1 | 85 | 1 | 85 | 1.6e-36 | 152.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071480.t1 | A0A0B2PBB9 | 100.0 | 85 | 0 | 0 | 1 | 85 | 1 | 85 | 1.6e-37 | 164.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene071480 | 0.841771 | 3.88E-58 | -1.69E-46 |
| MS.gene049255 | MS.gene071480 | 0.825243 | 5.21E-54 | -1.69E-46 |
| MS.gene049341 | MS.gene071480 | 0.815979 | 7.00E-52 | -1.69E-46 |
| MS.gene049501 | MS.gene071480 | 0.801451 | 8.95E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene071480 | 0.823644 | 1.24E-53 | -1.69E-46 |
| MS.gene049850 | MS.gene071480 | 0.814114 | 1.82E-51 | -1.69E-46 |
| MS.gene050020 | MS.gene071480 | 0.848273 | 6.81E-60 | -1.69E-46 |
| MS.gene050303 | MS.gene071480 | 0.833745 | 4.47E-56 | -1.69E-46 |
| MS.gene050438 | MS.gene071480 | 0.808361 | 3.22E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene071480 | 0.819774 | 9.73E-53 | -1.69E-46 |
| MS.gene050689 | MS.gene071480 | 0.800267 | 1.56E-48 | -1.69E-46 |
| MS.gene050719 | MS.gene071480 | 0.800487 | 1.41E-48 | -1.69E-46 |
| MS.gene050724 | MS.gene071480 | 0.809512 | 1.82E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene071480 | 0.823208 | 1.57E-53 | -1.69E-46 |
| MS.gene050815 | MS.gene071480 | 0.867742 | 1.09E-65 | -1.69E-46 |
| MS.gene051243 | MS.gene071480 | 0.818898 | 1.54E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene071480 | 0.811011 | 8.66E-51 | -1.69E-46 |
| MS.gene051328 | MS.gene071480 | 0.840334 | 9.26E-58 | -1.69E-46 |
| MS.gene05134 | MS.gene071480 | 0.814122 | 1.81E-51 | -1.69E-46 |
| MS.gene05157 | MS.gene071480 | 0.815223 | 1.03E-51 | -1.69E-46 |
| MS.gene051719 | MS.gene071480 | 0.820422 | 6.92E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene071480 | 0.800192 | 1.62E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene071480 | 0.806216 | 9.16E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene071480 | 0.827176 | 1.81E-54 | -1.69E-46 |
| MS.gene052020 | MS.gene071480 | 0.811448 | 6.97E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene071480 | 0.812435 | 4.25E-51 | -1.69E-46 |
| MS.gene052286 | MS.gene071480 | 0.806843 | 6.76E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene071480 | 0.800998 | 1.11E-48 | -1.69E-46 |
| MS.gene052519 | MS.gene071480 | 0.829401 | 5.25E-55 | -1.69E-46 |
| MS.gene052611 | MS.gene071480 | 0.837392 | 5.34E-57 | -1.69E-46 |
| MS.gene052863 | MS.gene071480 | 0.829527 | 4.90E-55 | -1.69E-46 |
| MS.gene052896 | MS.gene071480 | 0.801575 | 8.44E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene071480 | 0.8447 | 6.43E-59 | -1.69E-46 |
| MS.gene053226 | MS.gene071480 | 0.843017 | 1.81E-58 | -1.69E-46 |
| MS.gene05364 | MS.gene071480 | 0.812066 | 5.11E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene071480 | 0.806212 | 9.18E-50 | -1.69E-46 |
| MS.gene054651 | MS.gene071480 | 0.800568 | 1.36E-48 | -1.69E-46 |
| MS.gene054657 | MS.gene071480 | 0.807776 | 4.28E-50 | -1.69E-46 |
| MS.gene054658 | MS.gene071480 | 0.825798 | 3.85E-54 | -1.69E-46 |
| MS.gene054775 | MS.gene071480 | 0.811818 | 5.79E-51 | -1.69E-46 |
| MS.gene055166 | MS.gene071480 | 0.804042 | 2.61E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene071480 | 0.814379 | 1.59E-51 | -1.69E-46 |
| MS.gene055466 | MS.gene071480 | 0.83917 | 1.86E-57 | -1.69E-46 |
| MS.gene055468 | MS.gene071480 | 0.820103 | 8.18E-53 | -1.69E-46 |
| MS.gene055512 | MS.gene071480 | 0.813914 | 2.01E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene071480 | 0.806151 | 9.46E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene071480 | 0.810115 | 1.35E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene071480 | 0.817809 | 2.72E-52 | -1.69E-46 |
| MS.gene055808 | MS.gene071480 | 0.806558 | 7.76E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene071480 | 0.839622 | 1.42E-57 | -1.69E-46 |
| MS.gene05604 | MS.gene071480 | 0.801574 | 8.44E-49 | -1.69E-46 |
| MS.gene056215 | MS.gene071480 | 0.839601 | 1.44E-57 | -1.69E-46 |
| MS.gene056498 | MS.gene071480 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene056595 | MS.gene071480 | 0.812454 | 4.21E-51 | -1.69E-46 |
| MS.gene056631 | MS.gene071480 | 0.807052 | 6.10E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene071480 | 0.815607 | 8.48E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene071480 | 0.848581 | 5.59E-60 | -1.69E-46 |
| MS.gene057140 | MS.gene071480 | 0.807736 | 4.37E-50 | -1.69E-46 |
| MS.gene057314 | MS.gene071480 | 0.801413 | 9.11E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene071480 | 0.809915 | 1.49E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene071480 | 0.860522 | 1.95E-63 | -1.69E-46 |
| MS.gene057915 | MS.gene071480 | 0.812242 | 4.68E-51 | -1.69E-46 |
| MS.gene05795 | MS.gene071480 | 0.802217 | 6.23E-49 | -1.69E-46 |
| MS.gene058084 | MS.gene071480 | 0.821501 | 3.90E-53 | -1.69E-46 |
| MS.gene058146 | MS.gene071480 | 0.810479 | 1.13E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene071480 | 0.810933 | 9.01E-51 | -1.69E-46 |
| MS.gene058548 | MS.gene071480 | 0.812591 | 3.93E-51 | -1.69E-46 |
| MS.gene058549 | MS.gene071480 | 0.81395 | 1.97E-51 | -1.69E-46 |
| MS.gene058906 | MS.gene071480 | 0.823351 | 1.45E-53 | -1.69E-46 |
| MS.gene059151 | MS.gene071480 | 0.851496 | 8.55E-61 | -1.69E-46 |
| MS.gene05954 | MS.gene071480 | 0.820398 | 7.01E-53 | -1.69E-46 |
| MS.gene05979 | MS.gene071480 | 0.816473 | 5.43E-52 | -1.69E-46 |
| MS.gene059842 | MS.gene071480 | 0.825739 | 3.98E-54 | -1.69E-46 |
| MS.gene059937 | MS.gene071480 | 0.805059 | 1.60E-49 | -1.69E-46 |
| MS.gene060310 | MS.gene071480 | 0.803062 | 4.17E-49 | -1.69E-46 |
| MS.gene060322 | MS.gene071480 | 0.819876 | 9.23E-53 | -1.69E-46 |
| MS.gene060429 | MS.gene071480 | 0.869765 | 2.42E-66 | -1.69E-46 |
| MS.gene060572 | MS.gene071480 | 0.823535 | 1.31E-53 | -1.69E-46 |
| MS.gene060586 | MS.gene071480 | 0.801106 | 1.05E-48 | -1.69E-46 |
| MS.gene060802 | MS.gene071480 | 0.80837 | 3.20E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene071480 | 0.866899 | 2.03E-65 | -1.69E-46 |
| MS.gene060907 | MS.gene071480 | 0.812148 | 4.91E-51 | -1.69E-46 |
| MS.gene060917 | MS.gene071480 | 0.80562 | 1.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071480.t1 | MTR_5g030935 | 100.000 | 85 | 0 | 0 | 1 | 85 | 1 | 85 | 2.67e-57 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071480.t1 | AT2G22640 | 90.588 | 85 | 8 | 0 | 1 | 85 | 1 | 85 | 6.83e-53 | 159 |
Find 12 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAAATTCAGTACCGAAT+TGG | 0.368507 | 5.2:+20935627 | None:intergenic |
| TGATGTATTGGAGCGTCGTT+TGG | 0.385920 | 5.2:-20935470 | MS.gene071480:CDS |
| GAATCGCTGTTCAAGCTGAT+TGG | 0.386107 | 5.2:-20935712 | MS.gene071480:CDS |
| TTCGATTTCCTTGTCCAATT+CGG | 0.396293 | 5.2:-20935641 | MS.gene071480:intron |
| GAATGAAAAGCTTGATGTAT+TGG | 0.415813 | 5.2:-20935482 | MS.gene071480:CDS |
| AATCGCTGTTCAAGCTGATT+GGG | 0.456401 | 5.2:-20935711 | MS.gene071480:CDS |
| AGTTCAAGTTGGAAATGCTT+CGG | 0.460394 | 5.2:-20935437 | MS.gene071480:CDS |
| GAATTACTTGAAGTTCAAGT+TGG | 0.488446 | 5.2:-20935448 | MS.gene071480:CDS |
| ATTCCATCAATGGCACGTGC+TGG | 0.545650 | 5.2:-20935761 | None:intergenic |
| CCATCAATGGCACGTGCTGG+TGG | 0.566134 | 5.2:-20935758 | None:intergenic |
| ATTCAGTACCGAATTGGACA+AGG | 0.614288 | 5.2:+20935633 | None:intergenic |
| ATCACAAACGCAGTTAACGT+TGG | 0.620945 | 5.2:-20935734 | MS.gene071480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAGATTTCAAATAACA+AGG | + | chr5.2:20935592-20935611 | None:intergenic | 15.0% |
| !!! | AATCTTATATCTTTTTTTGA+TGG | - | chr5.2:20935602-20935621 | MS.gene071480:intron | 15.0% |
| !!! | ATCTTATATCTTTTTTTGAT+GGG | - | chr5.2:20935603-20935622 | MS.gene071480:intron | 15.0% |
| !!! | TGTTATTTTTGTTATTTCAG+AGG | - | chr5.2:20935630-20935649 | MS.gene071480:intron | 20.0% |
| ! | ATTTCAAATAACAAGGCAAA+AGG | + | chr5.2:20935585-20935604 | None:intergenic | 25.0% |
| ! | TTTCAAATAACAAGGCAAAA+GGG | + | chr5.2:20935584-20935603 | None:intergenic | 25.0% |
| GAATGAAAAGCTTGATGTAT+TGG | - | chr5.2:20935681-20935700 | MS.gene071480:CDS | 30.0% | |
| GAATTACTTGAAGTTCAAGT+TGG | - | chr5.2:20935715-20935734 | MS.gene071480:CDS | 30.0% | |
| TTCGATTTCCTTGTCCAATT+CGG | - | chr5.2:20935522-20935541 | MS.gene071480:intron | 35.0% | |
| ! | GAAGAAATTCAGTACCGAAT+TGG | + | chr5.2:20935539-20935558 | None:intergenic | 35.0% |
| !! | AGTTCAAGTTGGAAATGCTT+CGG | - | chr5.2:20935726-20935745 | MS.gene071480:CDS | 35.0% |
| AATCGCTGTTCAAGCTGATT+GGG | - | chr5.2:20935452-20935471 | MS.gene071480:CDS | 40.0% | |
| ATCACAAACGCAGTTAACGT+TGG | - | chr5.2:20935429-20935448 | MS.gene071480:CDS | 40.0% | |
| ! | ATTCAGTACCGAATTGGACA+AGG | + | chr5.2:20935533-20935552 | None:intergenic | 40.0% |
| GAATCGCTGTTCAAGCTGAT+TGG | - | chr5.2:20935451-20935470 | MS.gene071480:CDS | 45.0% | |
| !! | TGATGTATTGGAGCGTCGTT+TGG | - | chr5.2:20935693-20935712 | MS.gene071480:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 20935411 | 20935774 | 20935411 | ID=MS.gene071480 |
| chr5.2 | mRNA | 20935411 | 20935774 | 20935411 | ID=MS.gene071480.t1;Parent=MS.gene071480 |
| chr5.2 | exon | 20935642 | 20935774 | 20935642 | ID=MS.gene071480.t1.exon1;Parent=MS.gene071480.t1 |
| chr5.2 | CDS | 20935642 | 20935774 | 20935642 | ID=cds.MS.gene071480.t1;Parent=MS.gene071480.t1 |
| chr5.2 | exon | 20935411 | 20935535 | 20935411 | ID=MS.gene071480.t1.exon2;Parent=MS.gene071480.t1 |
| chr5.2 | CDS | 20935411 | 20935535 | 20935411 | ID=cds.MS.gene071480.t1;Parent=MS.gene071480.t1 |
| Gene Sequence |
| Protein sequence |
