AlfalfaGEDB | MS.gene08253

M. sativa cultivar XinJiangDaYe / MS.gene08253

KEGG: K00454
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08253.t1	XP_013446534.1	91.1	124	11	0	1	124	1	124	1.20E-47	199.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08253.t1	Q9CAG3	36.8	117	66	2	10	124	16	126	4.5e-06	52.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08253.t1	A0A072TTP2	91.1	124	11	0	1	124	1	124	9.0e-48	199.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene08253	0.831299	1.81E-55	-1.69E-46
MS.gene049552	MS.gene08253	0.823915	1.07E-53	-1.69E-46
MS.gene049656	MS.gene08253	0.816851	4.47E-52	-1.69E-46
MS.gene050122	MS.gene08253	0.834266	3.31E-56	-1.69E-46
MS.gene050170	MS.gene08253	0.822832	1.92E-53	-1.69E-46
MS.gene050496	MS.gene08253	0.8158	7.68E-52	-1.69E-46
MS.gene050499	MS.gene08253	0.832117	1.14E-55	-1.69E-46
MS.gene050502	MS.gene08253	0.866158	3.50E-65	-1.69E-46
MS.gene050520	MS.gene08253	0.813228	2.85E-51	-1.69E-46
MS.gene050747	MS.gene08253	0.814019	1.91E-51	-1.69E-46
MS.gene050868	MS.gene08253	0.808357	3.22E-50	-1.69E-46
MS.gene050871	MS.gene08253	0.810664	1.03E-50	-1.69E-46
MS.gene050950	MS.gene08253	0.810657	1.03E-50	-1.69E-46
MS.gene051509	MS.gene08253	0.846659	1.89E-59	-1.69E-46
MS.gene051560	MS.gene08253	0.817818	2.71E-52	-1.69E-46
MS.gene051941	MS.gene08253	0.850764	1.38E-60	-1.69E-46
MS.gene052066	MS.gene08253	0.80564	1.21E-49	-1.69E-46
MS.gene052164	MS.gene08253	0.830923	2.23E-55	-1.69E-46
MS.gene052346	MS.gene08253	0.844356	7.95E-59	-1.69E-46
MS.gene052413	MS.gene08253	0.830995	2.14E-55	-1.69E-46
MS.gene052482	MS.gene08253	0.822333	2.51E-53	-1.69E-46
MS.gene052486	MS.gene08253	0.83657	8.66E-57	-1.69E-46
MS.gene052503	MS.gene08253	0.810299	1.24E-50	-1.69E-46
MS.gene052823	MS.gene08253	0.811424	7.05E-51	-1.69E-46
MS.gene052825	MS.gene08253	0.807272	5.48E-50	-1.69E-46
MS.gene052966	MS.gene08253	0.816012	6.89E-52	-1.69E-46
MS.gene052999	MS.gene08253	0.806718	7.18E-50	-1.69E-46
MS.gene053203	MS.gene08253	0.826329	2.88E-54	-1.69E-46
MS.gene053213	MS.gene08253	0.800518	1.39E-48	-1.69E-46
MS.gene053286	MS.gene08253	0.80369	3.09E-49	-1.69E-46
MS.gene053318	MS.gene08253	0.833443	5.32E-56	-1.69E-46
MS.gene053436	MS.gene08253	0.857022	2.17E-62	-1.69E-46
MS.gene053574	MS.gene08253	0.839431	1.59E-57	-1.69E-46
MS.gene053626	MS.gene08253	0.83422	3.40E-56	-1.69E-46
MS.gene053633	MS.gene08253	0.80484	1.78E-49	-1.69E-46
MS.gene054595	MS.gene08253	0.830593	2.69E-55	-1.69E-46
MS.gene054600	MS.gene08253	0.801622	8.25E-49	-1.69E-46
MS.gene054927	MS.gene08253	0.805762	1.14E-49	-1.69E-46
MS.gene05492	MS.gene08253	0.801017	1.10E-48	-1.69E-46
MS.gene055722	MS.gene08253	0.801408	9.13E-49	-1.69E-46
MS.gene055741	MS.gene08253	0.806982	6.31E-50	-1.69E-46
MS.gene05589	MS.gene08253	0.8083	3.31E-50	-1.69E-46
MS.gene055909	MS.gene08253	0.848934	4.46E-60	-1.69E-46
MS.gene055911	MS.gene08253	0.847901	8.62E-60	-1.69E-46
MS.gene05601	MS.gene08253	0.857986	1.12E-62	-1.69E-46
MS.gene056040	MS.gene08253	0.826076	3.31E-54	-1.69E-46
MS.gene056166	MS.gene08253	0.847839	8.97E-60	-1.69E-46
MS.gene056346	MS.gene08253	0.820658	6.10E-53	-1.69E-46
MS.gene056504	MS.gene08253	0.828298	9.72E-55	-1.69E-46
MS.gene056510	MS.gene08253	0.813249	2.82E-51	-1.69E-46
MS.gene05666	MS.gene08253	0.82247	2.33E-53	-1.69E-46
MS.gene056878	MS.gene08253	0.824161	9.37E-54	-1.69E-46
MS.gene057019	MS.gene08253	0.83332	5.71E-56	-1.69E-46
MS.gene057086	MS.gene08253	0.81671	4.81E-52	-1.69E-46
MS.gene057734	MS.gene08253	0.818809	1.61E-52	-1.69E-46
MS.gene05787	MS.gene08253	0.837544	4.88E-57	-1.69E-46
MS.gene058120	MS.gene08253	0.809593	1.75E-50	-1.69E-46
MS.gene058157	MS.gene08253	0.814187	1.75E-51	-1.69E-46
MS.gene058183	MS.gene08253	0.805153	1.53E-49	-1.69E-46
MS.gene058420	MS.gene08253	0.824624	7.30E-54	-1.69E-46
MS.gene058948	MS.gene08253	0.809979	1.45E-50	-1.69E-46
MS.gene059129	MS.gene08253	0.813917	2.01E-51	-1.69E-46
MS.gene059303	MS.gene08253	0.818999	1.46E-52	-1.69E-46
MS.gene059367	MS.gene08253	0.858338	8.82E-63	-1.69E-46
MS.gene059368	MS.gene08253	0.846774	1.76E-59	-1.69E-46
MS.gene059517	MS.gene08253	0.867566	1.25E-65	-1.69E-46
MS.gene059680	MS.gene08253	0.84694	1.58E-59	-1.69E-46
MS.gene059718	MS.gene08253	0.804923	1.71E-49	-1.69E-46
MS.gene059865	MS.gene08253	0.802209	6.25E-49	-1.69E-46
MS.gene05995	MS.gene08253	0.814724	1.33E-51	-1.69E-46
MS.gene06000	MS.gene08253	0.833656	4.71E-56	-1.69E-46
MS.gene060187	MS.gene08253	-0.808233	3.43E-50	-1.69E-46
MS.gene060372	MS.gene08253	0.834338	3.17E-56	-1.69E-46
MS.gene060482	MS.gene08253	0.841321	5.10E-58	-1.69E-46
MS.gene060595	MS.gene08253	-0.810498	1.12E-50	-1.69E-46
MS.gene060778	MS.gene08253	0.858179	9.84E-63	-1.69E-46
MS.gene06077	MS.gene08253	0.820654	6.12E-53	-1.69E-46
MS.gene060837	MS.gene08253	0.822463	2.34E-53	-1.69E-46
MS.gene06097	MS.gene08253	0.802611	5.17E-49	-1.69E-46
MS.gene060982	MS.gene08253	0.822392	2.43E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08253.t1	MTR_8g080230	90.400	125	12	0	1	125	1	125	3.73e-47	162

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08253.t1	AT1G67560	36.752	117	66	2	10	124	16	126	1.65e-13	66.2

Find 32 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGTGTGGTTGAGGAGTTTG+AGG	0.343973	4.3:-16505625	MS.gene08253:CDS
TCTCCTCCGGTGCCTCTTAT+CGG	0.348322	4.3:+16505848	None:intergenic
TGAGGAGTTTGAGGATCAGT+TGG	0.424110	4.3:-16505616	MS.gene08253:CDS
TTCATCAATGGTGTTGGTCA+AGG	0.463683	4.3:-16505588	MS.gene08253:CDS
TTGCTCACTTTGCTGTCCAC+AGG	0.468287	4.3:+16505741	None:intergenic
AATGAAAGTTAGTGTGGTTG+AGG	0.473822	4.3:-16505634	MS.gene08253:CDS
CAGTTGGAGTATTTCATCAA+TGG	0.487178	4.3:-16505600	MS.gene08253:CDS
GAGTATTTCATCAATGGTGT+TGG	0.511662	4.3:-16505594	MS.gene08253:CDS
CGTCGGTGTTCTCCGATAAG+AGG	0.523684	4.3:-16505860	MS.gene08253:CDS
TCATCAATGGTGTTGGTCAA+GGG	0.527017	4.3:-16505587	MS.gene08253:CDS
CGGAGAACACCGACGGCGAT+CGG	0.542880	4.3:+16505868	None:intergenic
ACCGTGAAATGATTCTCCTC+CGG	0.555957	4.3:+16505835	None:intergenic
AAAGAAAATGAAAGTTAGTG+TGG	0.562354	4.3:-16505640	MS.gene08253:CDS
AAGCAACGGTGAAATGATAG+AGG	0.564793	4.3:-16505685	MS.gene08253:CDS
ACCGGAGGAGAATCATTTCA+CGG	0.579496	4.3:-16505836	MS.gene08253:CDS
GACTACGACGTCGTCTCCTG+TGG	0.583196	4.3:-16505757	MS.gene08253:CDS
TCATTTCACGGTCATCTGAA+CGG	0.584499	4.3:-16505824	MS.gene08253:CDS
TCGAGTGGTGGCAGAAGCAA+CGG	0.588302	4.3:-16505699	MS.gene08253:CDS
GTTACTCCTCCGATCGCCGT+CGG	0.593763	4.3:-16505877	MS.gene08253:CDS
GTTAGTGAACTCATAGACCC+TGG	0.596456	4.3:-16505549	MS.gene08253:intron
GACGGCGATCGGAGGAGTAA+CGG	0.598155	4.3:+16505879	None:intergenic
TGTTCTCCGATAAGAGGCAC+CGG	0.614102	4.3:-16505854	MS.gene08253:CDS
AGAACACCGACGGCGATCGG+AGG	0.619515	4.3:+16505871	None:intergenic
GTGGACAGCAAAGTGAGCAA+TGG	0.631784	4.3:-16505738	MS.gene08253:CDS
CAAGTTCAAGCAGTGATCAG+TGG	0.636193	4.3:-16505792	MS.gene08253:CDS
TCGTTACAAAGTAGCTCGAG+TGG	0.638320	4.3:-16505714	MS.gene08253:CDS
CTCTTATCGGAGAACACCGA+CGG	0.651240	4.3:+16505861	None:intergenic
TTACAAAGTAGCTCGAGTGG+TGG	0.661157	4.3:-16505711	MS.gene08253:CDS
GTTCAAGCAGTGATCAGTGG+CGG	0.666328	4.3:-16505789	MS.gene08253:CDS
TCTCCGATAAGAGGCACCGG+AGG	0.691008	4.3:-16505851	MS.gene08253:CDS
AATGATAGAGGTGAAAGCAG+TGG	0.733351	4.3:-16505673	MS.gene08253:CDS
TTTCACGGTCATCTGAACGG+CGG	0.756617	4.3:-16505821	MS.gene08253:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAATTAAGTTAGAGAATACT+CGG	-	chr4.3:16505871-16505890	MS.gene08253:CDS	20.0%
!!	TGATGAATTAAAAGAAGAAA+AGG	+	chr4.3:16505721-16505740	None:intergenic	20.0%
!!!	CTTCTTTTAATTCATCAAAA+AGG	-	chr4.3:16505724-16505743	MS.gene08253:CDS	20.0%
!	AAAGAAAATGAAAGTTAGTG+TGG	-	chr4.3:16505538-16505557	MS.gene08253:intron	25.0%
!	ATATTAAGAAGAAACAAACC+AGG	+	chr4.3:16505650-16505669	None:intergenic	25.0%
!	TATTAAGAAGAAACAAACCA+GGG	+	chr4.3:16505649-16505668	None:intergenic	25.0%
	AAAGAAGAAAAGGAGAGATA+TGG	+	chr4.3:16505711-16505730	None:intergenic	30.0%
!	AAAAAAAGTGTGTGATTGTG+TGG	+	chr4.3:16505756-16505775	None:intergenic	30.0%
!	AAAAAAGTGTGTGATTGTGT+GGG	+	chr4.3:16505755-16505774	None:intergenic	30.0%
	AATGAAAGTTAGTGTGGTTG+AGG	-	chr4.3:16505544-16505563	MS.gene08253:intron	35.0%
	CTTGTGTGATTCAAATGAAG+AGG	-	chr4.3:16505819-16505838	MS.gene08253:CDS	35.0%
	GAGTATTTCATCAATGGTGT+TGG	-	chr4.3:16505584-16505603	MS.gene08253:CDS	35.0%
!	CAGTTGGAGTATTTCATCAA+TGG	-	chr4.3:16505578-16505597	MS.gene08253:CDS	35.0%
	AAGCAACGGTGAAATGATAG+AGG	-	chr4.3:16505493-16505512	MS.gene08253:intron	40.0%
	AATGATAGAGGTGAAAGCAG+TGG	-	chr4.3:16505505-16505524	MS.gene08253:intron	40.0%
	GTTAGAGAATACTCGGATCT+AGG	-	chr4.3:16505878-16505897	MS.gene08253:CDS	40.0%
	TCATTTCACGGTCATCTGAA+CGG	-	chr4.3:16505354-16505373	MS.gene08253:intron	40.0%
!	TCTAGGCGCTTTTAGATCTT+TGG	-	chr4.3:16505895-16505914	MS.gene08253:CDS	40.0%
!!	TCATCAATGGTGTTGGTCAA+GGG	-	chr4.3:16505591-16505610	MS.gene08253:CDS	40.0%
!!	TTCATCAATGGTGTTGGTCA+AGG	-	chr4.3:16505590-16505609	MS.gene08253:CDS	40.0%
	ACCGGAGGAGAATCATTTCA+CGG	-	chr4.3:16505342-16505361	MS.gene08253:intron	45.0%
	ACCGTGAAATGATTCTCCTC+CGG	+	chr4.3:16505346-16505365	None:intergenic	45.0%
	CAAGTTCAAGCAGTGATCAG+TGG	-	chr4.3:16505386-16505405	MS.gene08253:intron	45.0%
	GTTAGTGAACTCATAGACCC+TGG	-	chr4.3:16505629-16505648	MS.gene08253:CDS	45.0%
	TAGTGTGGTTGAGGAGTTTG+AGG	-	chr4.3:16505553-16505572	MS.gene08253:CDS	45.0%
	TCGTTACAAAGTAGCTCGAG+TGG	-	chr4.3:16505464-16505483	MS.gene08253:intron	45.0%
	TGAGGAGTTTGAGGATCAGT+TGG	-	chr4.3:16505562-16505581	MS.gene08253:CDS	45.0%
	TTACAAAGTAGCTCGAGTGG+TGG	-	chr4.3:16505467-16505486	MS.gene08253:intron	45.0%
!!!	AAATAATTTATTTTAAAAAG+AGG	+	chr4.3:16505783-16505802	None:intergenic	5.0%
	CTCTTATCGGAGAACACCGA+CGG	+	chr4.3:16505320-16505339	None:intergenic	50.0%
	GTGGACAGCAAAGTGAGCAA+TGG	-	chr4.3:16505440-16505459	MS.gene08253:intron	50.0%
	GTTCAAGCAGTGATCAGTGG+CGG	-	chr4.3:16505389-16505408	MS.gene08253:intron	50.0%
	TTTCACGGTCATCTGAACGG+CGG	-	chr4.3:16505357-16505376	MS.gene08253:intron	50.0%
!	TGTTCTCCGATAAGAGGCAC+CGG	-	chr4.3:16505324-16505343	MS.gene08253:intron	50.0%
!	TTGCTCACTTTGCTGTCCAC+AGG	+	chr4.3:16505440-16505459	None:intergenic	50.0%
	TCGAGTGGTGGCAGAAGCAA+CGG	-	chr4.3:16505479-16505498	MS.gene08253:intron	55.0%
	TCTCCTCCGGTGCCTCTTAT+CGG	+	chr4.3:16505333-16505352	None:intergenic	55.0%
!	CGTCGGTGTTCTCCGATAAG+AGG	-	chr4.3:16505318-16505337	MS.gene08253:intron	55.0%
	GACGGCGATCGGAGGAGTAA+CGG	+	chr4.3:16505302-16505321	None:intergenic	60.0%
	GACTACGACGTCGTCTCCTG+TGG	-	chr4.3:16505421-16505440	MS.gene08253:intron	60.0%
	GTTACTCCTCCGATCGCCGT+CGG	-	chr4.3:16505301-16505320	MS.gene08253:intron	60.0%
!!	TCTCCGATAAGAGGCACCGG+AGG	-	chr4.3:16505327-16505346	MS.gene08253:intron	60.0%
	AGAACACCGACGGCGATCGG+AGG	+	chr4.3:16505310-16505329	None:intergenic	65.0%
	CGGAGAACACCGACGGCGAT+CGG	+	chr4.3:16505313-16505332	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	16505278	16505922	16505278	ID=MS.gene08253
chr4.3	mRNA	16505278	16505922	16505278	ID=MS.gene08253.t1;Parent=MS.gene08253
chr4.3	exon	16505550	16505922	16505550	ID=MS.gene08253.t1.exon1;Parent=MS.gene08253.t1
chr4.3	CDS	16505550	16505922	16505550	ID=cds.MS.gene08253.t1;Parent=MS.gene08253.t1
chr4.3	exon	16505278	16505300	16505278	ID=MS.gene08253.t1.exon2;Parent=MS.gene08253.t1
chr4.3	CDS	16505278	16505300	16505278	ID=cds.MS.gene08253.t1;Parent=MS.gene08253.t1

Gene Sequence

>MS.gene08253

ATGTTCGCAGTGAAGTCTAATCCGTTACTCCTCCGATCGCCGTCGGTGTTCTCCGATAAGAGGCACCGGAGGAGAATCATTTCACGGTCATCTGAACGGCGGAGCGTTCAAGTTCAAGCAGTGATCAGTGGCGGAGACAGCAAGACTACGACGTCGTCTCCTGTGGACAGCAAAGTGAGCAATGGCTCGTTACAAAGTAGCTCGAGTGGTGGCAGAAGCAACGGTGAAATGATAGAGGTGAAAGCAGTGGTGATGATAAGAAAGAAAATGAAAGTTAGTGTGGTTGAGGAGTTTGAGGATCAGTTGGAGTATTTCATCAATGGTGTTGGTCAAGGGATACAGATTCAGCTTGTTAGTGAACTCATAGACCCTGGTTTGTTTCTTCTTAATATTATACTATTATTTTCTCTATTCTTCTATATAATTCAGTCCATATCTCTCCTTTTCTTCTTTTAATTCATCAAAAAGGAGTGTCCCACACAATCACACACTTTTTTTTAAGCCTCTTTTTAAAATAAATTATTTTCATGACATATCGAGTCTTGTGTGATTCAAATGAAGAGGTTTAAAGTTTAAATTCAAACTTGAAATCTTAATTAAGTTAGAGAATACTCGGATCTAGGCGCTTTTAGATCTTTGGTTTAA

Protein sequence

>MS.gene08253.t1

MFAVKSNPLLLRSPSVFSDKRHRRRIISRSSERRSVQVQAVISGGDSKTTTSSPVDSKVSNGSLQSSSSGGRSNGEMIEVKAVVMIRKKMKVSVVEEFEDQLEYFINGVGQGIQIQLVSELIDPGAFRSLV