AlfalfaGEDB | MS.gene22091

M. sativa cultivar XinJiangDaYe / MS.gene22091

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF13041.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22091.t1	XP_011462354.1	34.3	140	82	3	50	187	198	329	1.60E-09	73.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22091.t1	O65543	29.1	141	88	4	46	183	313	444	7.4e-08	58.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22091.t1	A0A1Q3D554	31.6	155	93	4	38	187	51	197	5.6e-09	70.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene22091	0.812118	4.98E-51	-1.69E-46
MS.gene049159	MS.gene22091	0.884517	1.79E-71	-1.69E-46
MS.gene049435	MS.gene22091	0.84227	2.86E-58	-1.69E-46
MS.gene049452	MS.gene22091	0.801124	1.04E-48	-1.69E-46
MS.gene049501	MS.gene22091	0.803847	2.87E-49	-1.69E-46
MS.gene049647	MS.gene22091	0.816476	5.42E-52	-1.69E-46
MS.gene049801	MS.gene22091	0.81867	1.74E-52	-1.69E-46
MS.gene050442	MS.gene22091	0.834384	3.09E-56	-1.69E-46
MS.gene050605	MS.gene22091	0.844414	7.67E-59	-1.69E-46
MS.gene050680	MS.gene22091	0.802214	6.24E-49	-1.69E-46
MS.gene050815	MS.gene22091	0.816134	6.47E-52	-1.69E-46
MS.gene050844	MS.gene22091	0.820445	6.83E-53	-1.69E-46
MS.gene051066	MS.gene22091	0.817946	2.53E-52	-1.69E-46
MS.gene051180	MS.gene22091	0.80367	3.12E-49	-1.69E-46
MS.gene051198	MS.gene22091	0.834538	2.83E-56	-1.69E-46
MS.gene051210	MS.gene22091	0.800812	1.21E-48	-1.69E-46
MS.gene051368	MS.gene22091	0.814659	1.38E-51	-1.69E-46
MS.gene051452	MS.gene22091	0.820855	5.50E-53	-1.69E-46
MS.gene051800	MS.gene22091	0.832958	7.02E-56	-1.69E-46
MS.gene052012	MS.gene22091	0.801247	9.85E-49	-1.69E-46
MS.gene052426	MS.gene22091	0.829949	3.87E-55	-1.69E-46
MS.gene052611	MS.gene22091	0.80364	3.17E-49	-1.69E-46
MS.gene052677	MS.gene22091	0.801776	7.68E-49	-1.69E-46
MS.gene052863	MS.gene22091	0.843567	1.29E-58	-1.69E-46
MS.gene05288	MS.gene22091	0.89563	7.98E-76	-1.69E-46
MS.gene052896	MS.gene22091	0.805675	1.19E-49	-1.69E-46
MS.gene05289	MS.gene22091	0.878581	2.50E-69	-1.69E-46
MS.gene053008	MS.gene22091	0.855635	5.53E-62	-1.69E-46
MS.gene053166	MS.gene22091	0.819564	1.09E-52	-1.69E-46
MS.gene053494	MS.gene22091	0.802985	4.33E-49	-1.69E-46
MS.gene053584	MS.gene22091	0.806465	8.12E-50	-1.69E-46
MS.gene054236	MS.gene22091	0.843592	1.27E-58	-1.69E-46
MS.gene054467	MS.gene22091	0.80634	8.63E-50	-1.69E-46
MS.gene054472	MS.gene22091	0.837422	5.25E-57	-1.69E-46
MS.gene054657	MS.gene22091	0.80869	2.74E-50	-1.69E-46
MS.gene054658	MS.gene22091	0.8266	2.48E-54	-1.69E-46
MS.gene054775	MS.gene22091	0.811364	7.27E-51	-1.69E-46
MS.gene054801	MS.gene22091	0.802624	5.14E-49	-1.69E-46
MS.gene05510	MS.gene22091	0.861425	1.04E-63	-1.69E-46
MS.gene055227	MS.gene22091	0.809841	1.55E-50	-1.69E-46
MS.gene055228	MS.gene22091	0.809934	1.48E-50	-1.69E-46
MS.gene055310	MS.gene22091	0.808702	2.72E-50	-1.69E-46
MS.gene055608	MS.gene22091	0.834564	2.79E-56	-1.69E-46
MS.gene055623	MS.gene22091	0.834726	2.54E-56	-1.69E-46
MS.gene055701	MS.gene22091	0.847879	8.75E-60	-1.69E-46
MS.gene055818	MS.gene22091	0.834252	3.34E-56	-1.69E-46
MS.gene055875	MS.gene22091	0.806671	7.35E-50	-1.69E-46
MS.gene055926	MS.gene22091	0.843919	1.04E-58	-1.69E-46
MS.gene056174	MS.gene22091	0.806921	6.50E-50	-1.69E-46
MS.gene056252	MS.gene22091	0.819526	1.11E-52	-1.69E-46
MS.gene056506	MS.gene22091	0.800045	1.73E-48	-1.69E-46
MS.gene056784	MS.gene22091	0.812107	5.01E-51	-1.69E-46
MS.gene056873	MS.gene22091	0.808748	2.66E-50	-1.69E-46
MS.gene057194	MS.gene22091	0.80321	3.89E-49	-1.69E-46
MS.gene057394	MS.gene22091	0.805611	1.23E-49	-1.69E-46
MS.gene057693	MS.gene22091	0.818083	2.36E-52	-1.69E-46
MS.gene057750	MS.gene22091	0.801112	1.05E-48	-1.69E-46
MS.gene057855	MS.gene22091	0.818255	2.16E-52	-1.69E-46
MS.gene057869	MS.gene22091	0.841885	3.62E-58	-1.69E-46
MS.gene057915	MS.gene22091	0.816963	4.22E-52	-1.69E-46
MS.gene058010	MS.gene22091	0.85123	1.02E-60	-1.69E-46
MS.gene058146	MS.gene22091	0.832079	1.16E-55	-1.69E-46
MS.gene058292	MS.gene22091	0.802286	6.03E-49	-1.69E-46
MS.gene058548	MS.gene22091	0.824779	6.71E-54	-1.69E-46
MS.gene058595	MS.gene22091	0.87863	2.40E-69	-1.69E-46
MS.gene058783	MS.gene22091	0.802828	4.66E-49	-1.69E-46
MS.gene058979	MS.gene22091	0.825028	5.86E-54	-1.69E-46
MS.gene059124	MS.gene22091	0.804126	2.51E-49	-1.69E-46
MS.gene059128	MS.gene22091	0.822536	2.25E-53	-1.69E-46
MS.gene059161	MS.gene22091	0.802244	6.15E-49	-1.69E-46
MS.gene059283	MS.gene22091	0.827211	1.77E-54	-1.69E-46
MS.gene059439	MS.gene22091	0.810996	8.73E-51	-1.69E-46
MS.gene059550	MS.gene22091	0.811286	7.55E-51	-1.69E-46
MS.gene05973	MS.gene22091	0.825172	5.42E-54	-1.69E-46
MS.gene05979	MS.gene22091	0.80497	1.67E-49	-1.69E-46
MS.gene059842	MS.gene22091	0.860565	1.89E-63	-1.69E-46
MS.gene060011	MS.gene22091	0.854361	1.30E-61	-1.69E-46
MS.gene060167	MS.gene22091	0.830621	2.65E-55	-1.69E-46
MS.gene060296	MS.gene22091	0.821056	4.95E-53	-1.69E-46
MS.gene060310	MS.gene22091	0.844271	8.38E-59	-1.69E-46
MS.gene060322	MS.gene22091	0.836816	7.50E-57	-1.69E-46
MS.gene06052	MS.gene22091	0.820658	6.11E-53	-1.69E-46
MS.gene060683	MS.gene22091	0.846799	1.73E-59	-1.69E-46
MS.gene060685	MS.gene22091	0.848203	7.12E-60	-1.69E-46
MS.gene060802	MS.gene22091	0.856367	3.37E-62	-1.69E-46
MS.gene060803	MS.gene22091	0.851547	8.27E-61	-1.69E-46
MS.gene060998	MS.gene22091	0.859361	4.36E-63	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22091.t1	MTR_7g011100	27.273	165	114	3	38	201	212	371	7.69e-16	75.5
MS.gene22091.t1	MTR_4g113830	27.273	165	104	5	38	197	204	357	6.74e-14	70.1
MS.gene22091.t1	MTR_4g130500	29.104	134	89	2	50	183	364	491	1.01e-13	69.3
MS.gene22091.t1	MTR_7g109860	27.027	148	100	3	38	184	210	350	2.67e-13	68.2
MS.gene22091.t1	MTR_5g006420	28.090	178	104	5	10	183	277	434	4.29e-13	67.4
MS.gene22091.t1	MTR_7g093900	27.411	197	133	5	4	198	102	290	1.66e-12	65.9
MS.gene22091.t1	MTR_7g093900	27.211	147	100	3	38	183	268	408	2.47e-11	62.4
MS.gene22091.t1	MTR_7g093900	27.411	197	133	5	4	198	152	340	1.80e-12	65.9
MS.gene22091.t1	MTR_7g093900	29.412	136	89	3	49	183	329	458	3.23e-11	62.0
MS.gene22091.t1	MTR_6g066360	29.530	149	97	4	46	193	267	408	3.28e-12	65.1
MS.gene22091.t1	MTR_3g080230	30.065	153	89	5	50	196	194	334	5.07e-12	64.3
MS.gene22091.t1	MTR_0011s0300	27.397	146	101	2	38	183	188	328	8.49e-12	63.5
MS.gene22091.t1	MTR_5g018690	29.078	141	90	4	48	186	186	318	9.99e-12	63.5
MS.gene22091.t1	MTR_4g108030	27.389	157	107	3	38	193	230	380	1.02e-11	63.5
MS.gene22091.t1	MTR_4g074470	27.389	157	107	3	38	193	228	378	1.04e-11	63.5
MS.gene22091.t1	MTR_8g074780	26.984	189	121	7	6	187	26	204	1.14e-11	63.2
MS.gene22091.t1	MTR_4g032895	26.087	138	96	4	50	186	599	731	1.64e-11	63.2
MS.gene22091.t1	MTR_7g056073	26.531	147	101	3	38	183	183	323	1.96e-11	62.8
MS.gene22091.t1	MTR_7g011030	24.855	173	107	6	38	201	269	427	2.37e-11	62.4
MS.gene22091.t1	MTR_6g018720	25.749	167	108	4	38	197	219	376	6.76e-11	61.2
MS.gene22091.t1	MTR_1g039030	26.471	170	108	4	38	201	452	610	9.91e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene22091.t1	AT1G26900	30.058	173	111	7	18	187	146	311	9.76e-15	72.4
MS.gene22091.t1	AT1G13410	31.061	132	85	3	53	183	143	269	3.34e-13	67.8
MS.gene22091.t1	AT1G14470	24.599	187	111	3	38	196	205	389	9.96e-13	66.6
MS.gene22091.t1	AT2G13600	24.022	179	122	4	7	183	306	472	4.69e-12	64.7
MS.gene22091.t1	AT3G09040	23.226	155	113	4	34	187	668	817	7.77e-12	63.9
MS.gene22091.t1	AT3G09040	23.226	155	113	4	34	187	668	817	7.77e-12	63.9
MS.gene22091.t1	AT3G09040	23.226	155	113	4	34	187	693	842	8.57e-12	63.9
MS.gene22091.t1	AT3G09040	23.226	155	113	4	34	187	693	842	8.57e-12	63.9
MS.gene22091.t1	AT3G15130	28.082	146	99	3	38	183	48	187	1.47e-11	63.2
MS.gene22091.t1	AT2G29760	31.429	140	79	6	50	183	83	211	1.63e-11	63.2
MS.gene22091.t1	AT3G26630	28.058	139	94	2	49	187	203	335	2.17e-11	62.4
MS.gene22091.t1	AT5G40405	29.710	138	89	3	50	186	193	323	5.43e-11	61.6
MS.gene22091.t1	AT5G40405	29.710	138	89	3	50	186	193	323	5.43e-11	61.6

Find 21 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTCATTGCATGCTATTTC+TGG	0.092217	3.4:+43723852	MS.gene22091:CDS
CATCAAGGCCTCAAAGAAAT+CGG	0.303809	3.4:+43724118	MS.gene22091:CDS
CAAACAAATTTAAGACATCT+TGG	0.370571	3.4:-43724043	None:intergenic
TATGTCTTCAACTTTAAGTA+TGG	0.384058	3.4:-43724153	None:intergenic
AGAAGGTATGTGTCTTCATT+AGG	0.413719	3.4:-43724092	None:intergenic
TCAAAGAAATCGGGTTTACT+TGG	0.420047	3.4:+43724128	MS.gene22091:CDS
AAGTAAACCCGATTTCTTTG+AGG	0.441197	3.4:-43724126	None:intergenic
TGCACACAACGGTATGTTTG+AGG	0.443354	3.4:+43724340	MS.gene22091:CDS
ATCAAGGCCTCAAAGAAATC+GGG	0.450825	3.4:+43724119	MS.gene22091:CDS
TTGAGGCCTTGATGAAAAGA+AGG	0.459610	3.4:-43724109	None:intergenic
AATGTGATGGTGGATCTCTT+TGG	0.470527	3.4:+43724227	MS.gene22091:CDS
TAGACAGTTCTAGCACTAGA+AGG	0.472435	3.4:-43723975	None:intergenic
GAGCTCAATGAGTCAAAGAC+AGG	0.538740	3.4:-43723834	None:intergenic
CTAGCGACTGTAACAGTGCA+TGG	0.540147	3.4:+43724255	MS.gene22091:CDS
GAGCTCAAAGAGTCAAAGAC+AGG	0.558436	3.4:+43723715	MS.gene22091:CDS
TCAATGAGTCAAAGACAGGT+TGG	0.606266	3.4:-43723830	None:intergenic
TAGCGACTGTAACAGTGCAT+GGG	0.618955	3.4:+43724256	MS.gene22091:CDS
GTGCTAGAACTGTCTATGTG+TGG	0.636362	3.4:+43723982	MS.gene22091:CDS
TAGATCTGAAGGCTTGTCGT+TGG	0.651266	3.4:-43724198	None:intergenic
GATGGTGGATCTCTTTGGAA+AGG	0.665284	3.4:+43724232	MS.gene22091:CDS
CTATTTCTGGAAGATCACAG+TGG	0.747490	3.4:+43723865	MS.gene22091:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATACATATTTTTCTCTTTGA+TGG	-	chr3.4:43724294-43724313	None:intergenic	20.0%
!	AACCATTTATTGTCAAACTT+GGG	+	chr3.4:43723796-43723815	MS.gene22091:intron	25.0%
!	AAGAGAAAAATATGTATTCG+TGG	+	chr3.4:43724297-43724316	MS.gene22091:CDS	25.0%
!	TATGTCTTCAACTTTAAGTA+TGG	-	chr3.4:43724156-43724175	None:intergenic	25.0%
!!	CAAACAAATTTAAGACATCT+TGG	-	chr3.4:43724046-43724065	None:intergenic	25.0%
	AGATCTATGCAAAAATGTGA+TGG	+	chr3.4:43724214-43724233	MS.gene22091:CDS	30.0%
	CAACCATTTATTGTCAAACT+TGG	+	chr3.4:43723795-43723814	MS.gene22091:intron	30.0%
!	ACATTTTTGCATAGATCTGA+AGG	-	chr3.4:43724212-43724231	None:intergenic	30.0%
	AAGTAAACCCGATTTCTTTG+AGG	-	chr3.4:43724129-43724148	None:intergenic	35.0%
	GACAATAAATGGTTGAACCT+TGG	-	chr3.4:43723790-43723809	None:intergenic	35.0%
	TCAAAGAAATCGGGTTTACT+TGG	+	chr3.4:43724128-43724147	MS.gene22091:CDS	35.0%
	TCTATGCAAAAATGTGATGG+TGG	+	chr3.4:43724217-43724236	MS.gene22091:CDS	35.0%
	TGCCCAAGTTTGACAATAAA+TGG	-	chr3.4:43723801-43723820	None:intergenic	35.0%
!	ACTCAAGTACTGTCAAACTT+GGG	-	chr3.4:43723756-43723775	None:intergenic	35.0%
!	AGAAGGTATGTGTCTTCATT+AGG	-	chr3.4:43724095-43724114	None:intergenic	35.0%
!	CACATACCTTCTTTTCATCA+AGG	+	chr3.4:43724103-43724122	MS.gene22091:CDS	35.0%
	ATCAAGGCCTCAAAGAAATC+GGG	+	chr3.4:43724119-43724138	MS.gene22091:CDS	40.0%
	CAAGTTTGACAGTACTTGAG+TGG	+	chr3.4:43723755-43723774	MS.gene22091:intron	40.0%
	CATCAAGGCCTCAAAGAAAT+CGG	+	chr3.4:43724118-43724137	MS.gene22091:CDS	40.0%
	TCAAAGAGTCAAAGACAGGT+TGG	+	chr3.4:43723719-43723738	MS.gene22091:intron	40.0%
	TCAATGAGTCAAAGACAGGT+TGG	-	chr3.4:43723833-43723852	None:intergenic	40.0%
	TTGAGGCCTTGATGAAAAGA+AGG	-	chr3.4:43724112-43724131	None:intergenic	40.0%
!	CACTCAAGTACTGTCAAACT+TGG	-	chr3.4:43723757-43723776	None:intergenic	40.0%
!	TAGACAGTTCTAGCACTAGA+AGG	-	chr3.4:43723978-43723997	None:intergenic	40.0%
!!	AATGTGATGGTGGATCTCTT+TGG	+	chr3.4:43724227-43724246	MS.gene22091:CDS	40.0%
!!	AGCTCATTGCATGCTATTTC+TGG	+	chr3.4:43723852-43723871	MS.gene22091:CDS	40.0%
!!	CTATTTCTGGAAGATCACAG+TGG	+	chr3.4:43723865-43723884	MS.gene22091:CDS	40.0%
!!	TTGAGTGGATTGAATCACCA+AGG	+	chr3.4:43723770-43723789	MS.gene22091:intron	40.0%
	GAGCTCAAAGAGTCAAAGAC+AGG	+	chr3.4:43723715-43723734	MS.gene22091:CDS	45.0%
	GAGCTCAATGAGTCAAAGAC+AGG	-	chr3.4:43723837-43723856	None:intergenic	45.0%
	GTGCTAGAACTGTCTATGTG+TGG	+	chr3.4:43723982-43724001	MS.gene22091:CDS	45.0%
	TAGATCTGAAGGCTTGTCGT+TGG	-	chr3.4:43724201-43724220	None:intergenic	45.0%
	TAGCGACTGTAACAGTGCAT+GGG	+	chr3.4:43724256-43724275	MS.gene22091:CDS	45.0%
	TGCACACAACGGTATGTTTG+AGG	+	chr3.4:43724340-43724359	MS.gene22091:CDS	45.0%
!!	CTGACTGCTTTTGCACACAA+CGG	+	chr3.4:43724329-43724348	MS.gene22091:CDS	45.0%
!!	GATGGTGGATCTCTTTGGAA+AGG	+	chr3.4:43724232-43724251	MS.gene22091:CDS	45.0%
	CTAGCGACTGTAACAGTGCA+TGG	+	chr3.4:43724255-43724274	MS.gene22091:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	43723713	43724409	43723713	ID=MS.gene22091
chr3.4	mRNA	43723713	43724409	43723713	ID=MS.gene22091.t1;Parent=MS.gene22091
chr3.4	exon	43723713	43723736	43723713	ID=MS.gene22091.t1.exon1;Parent=MS.gene22091.t1
chr3.4	CDS	43723713	43723736	43723713	ID=cds.MS.gene22091.t1;Parent=MS.gene22091.t1
chr3.4	exon	43723828	43724409	43723828	ID=MS.gene22091.t1.exon2;Parent=MS.gene22091.t1
chr3.4	CDS	43723828	43724409	43723828	ID=cds.MS.gene22091.t1;Parent=MS.gene22091.t1

Gene Sequence

>MS.gene22091

ATGAGCTCAAAGAGTCAAAGACAGGTTGGTGCTGAAAATGCCCAAGTTTGACAGTACTTGAGTGGATTGAATCACCAAGGTTCAACCATTTATTGTCAAACTTGGGCATTTTCAGCACCAACCTGTCTTTGACTCATTGAGCTCATTGCATGCTATTTCTGGAAGATCACAGTGGTTTGTATCTACAGAAGCTATTCACAAGATTTCACACTTTTCAGCTCAAAGAAATACTACGTCAGCACGACTAGTTTTCAATGAGCTTCCTTCTAGTGCTAGAACTGTCTATGTGTGGAATTCGATGATGAATGCTTATATCATGTCTCGTCAGAACCAAGATGTCTTAAATTTGTTTGAACTAATGAATGAAACTTCTTGTGTGCCTAATGAAGACACATACCTTCTTTTCATCAAGGCCTCAAAGAAATCGGGTTTACTTGGTACCATACTTAAAGTTGAAGACATAGAGAAGCATATGCTTCATGTCACCAACGACAAGCCTTCAGATCTATGCAAAAATGTGATGGTGGATCTCTTTGGAAAGGCTAGCGACTGTAACAGTGCATGGGAGTATTTCTGTTCCATCAAAGAGAAAAATATGTATTCGTGGAATTCTATGCTGACTGCTTTTGCACACAACGGTATGTTTGAGGACTATGAATCTTTTTCAGAGAATGCTATCAGAAAATGTTGCCCCTAA

Protein sequence

>MS.gene22091.t1

MSSKSQRQHQPVFDSLSSLHAISGRSQWFVSTEAIHKISHFSAQRNTTSARLVFNELPSSARTVYVWNSMMNAYIMSRQNQDVLNLFELMNETSCVPNEDTYLLFIKASKKSGLLGTILKVEDIEKHMLHVTNDKPSDLCKNVMVDLFGKASDCNSAWEYFCSIKEKNMYSWNSMLTAFAHNGMFEDYESFSENAIRKCCP