AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36354


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36354.t1 MTR_2g101130 95.312 128 3 1 1 128 34 158 3.54e-86 249
MS.gene36354.t1 MTR_2g101120 84.375 128 12 3 1 128 34 153 3.40e-70 209
MS.gene36354.t1 MTR_8g014030 64.844 128 31 5 1 127 34 148 9.81e-52 162
MS.gene36354.t1 MTR_8g014010 64.615 130 33 6 1 128 34 152 7.41e-49 154
MS.gene36354.t1 MTR_8g014020 61.682 107 27 5 22 127 1 94 3.63e-38 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36354.t1 AT1G52140 42.446 139 58 6 3 131 35 161 6.65e-24 92.0
MS.gene36354.t1 AT3G16330 37.681 138 51 7 3 126 35 151 2.50e-15 69.3

Find 35 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCCCTTGATGCGGTAAAT+TGG 0.283815 2.2:-2267338 None:intergenic
TGCGGTGACGTTTCCCTATT+GGG 0.299531 2.2:-2267398 None:intergenic
TCTCCAGCACTGCCTGGATT+CGG 0.302018 2.2:+2267555 MS.gene36354:CDS
CTGCGGTGACGTTTCCCTAT+TGG 0.308179 2.2:-2267399 None:intergenic
AAACGCCGCAGCAAGCTTGC+CGG 0.321704 2.2:+2267228 MS.gene36354:CDS
CTCCAGCACTGCCTGGATTC+GGG 0.322990 2.2:+2267556 MS.gene36354:CDS
CGCCCGAATCCAGGCAGTGC+TGG 0.394166 2.2:-2267558 None:intergenic
GGCGTTTGAGCATCATGTTT+AGG 0.464704 2.2:-2267212 None:intergenic
CGTGGCTGTGGTGGCTGTGA+TGG 0.466817 2.2:-2267278 None:intergenic
ACATCATCGTCTTGTGTAGC+TGG 0.469440 2.2:-2267465 None:intergenic
GAGCGCAATGAAGGCCGTGC+TGG 0.471428 2.2:+2267497 MS.gene36354:CDS
TGAAGAAATTGTTGTGATTG+TGG 0.472141 2.2:-2267428 None:intergenic
AGGTGGAGCCGCCGTGGCTG+TGG 0.473691 2.2:-2267290 None:intergenic
CCCTTGATGCGGTAAATTGG+TGG 0.485419 2.2:-2267335 None:intergenic
GAAGCATCTCCAGCACTGCC+TGG 0.487263 2.2:+2267549 MS.gene36354:CDS
CATCACAGCCACCACAGCCA+CGG 0.499967 2.2:+2267279 MS.gene36354:CDS
GTCGTGAGAGCGGCTTGAGG+TGG 0.508313 2.2:-2267307 None:intergenic
ATTGGGCTACGCCCGAATCC+AGG 0.513644 2.2:-2267567 None:intergenic
TTACTGATTGATTAAGGCAA+AGG 0.523427 2.2:-2268724 None:intergenic
GGCTGTGGTGGCTGTGATGG+TGG 0.525762 2.2:-2267275 None:intergenic
GCCACCAATTTACCGCATCA+AGG 0.534674 2.2:+2267334 MS.gene36354:CDS
TGGAGCCGCCGTGGCTGTGG+TGG 0.543982 2.2:-2267287 None:intergenic
GTGGAACATTAGATTGGCAA+TGG 0.565737 2.2:-2267256 None:intergenic
GTGATGGTGGAACATTAGAT+TGG 0.566670 2.2:-2267262 None:intergenic
TGGATTCGGGCGTAGCCCAA+TGG 0.585672 2.2:+2267569 MS.gene36354:CDS
GGTGACAATGAGCGCAATGA+AGG 0.587801 2.2:+2267488 MS.gene36354:CDS
CCACCAATTTACCGCATCAA+GGG 0.590913 2.2:+2267335 MS.gene36354:CDS
GGCTTGAGGTGGAGCCGCCG+TGG 0.620681 2.2:-2267296 None:intergenic
GGTGGCGTGAGTCGTGAGAG+CGG 0.626926 2.2:-2267317 None:intergenic
TGTGATTGTGGTTGTTGCTG+CGG 0.635520 2.2:-2267416 None:intergenic
ATTGTTAAGCATCTCCAGCA+CGG 0.641871 2.2:-2267511 None:intergenic
CACAGCCACCACAGCCACGG+CGG 0.652559 2.2:+2267282 MS.gene36354:CDS
TGAGTCGTGAGAGCGGCTTG+AGG 0.683296 2.2:-2267310 None:intergenic
AGCTACACAAGACGATGATG+TGG 0.693812 2.2:+2267467 MS.gene36354:CDS
GAATTCATACTCCCTTGATG+CGG 0.696823 2.2:-2267346 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTGATTATTATTAAGAA+AGG - chr2.2:2268224-2268243 None:intergenic 15.0%
!! CATTAATTATTAACTAAACA+AGG - chr2.2:2267748-2267767 None:intergenic 15.0%
!! TTTAGTTAATAATTAATGTG+TGG + chr2.2:2267750-2267769 MS.gene36354:intron 15.0%
!!! AAAAATTTCAATGAATCTTT+TGG + chr2.2:2268572-2268591 MS.gene36354:intron 15.0%
!!! GTTTTTTCTCAAATAAATTA+CGG + chr2.2:2268004-2268023 MS.gene36354:intron 15.0%
!!! TATATATGAGATTCTTTTTA+AGG - chr2.2:2268076-2268095 None:intergenic 15.0%
!!! TTTTGTTAGAAATTATGAAA+AGG - chr2.2:2268039-2268058 None:intergenic 15.0%
!! AAACTTGAAATATGTGTTAA+GGG + chr2.2:2268371-2268390 MS.gene36354:intron 20.0%
!! AAATAACCAAAAATGCAAAA+TGG - chr2.2:2267802-2267821 None:intergenic 20.0%
!! AAGAATCTCATATATATGTT+TGG + chr2.2:2268080-2268099 MS.gene36354:intron 20.0%
!! AATTATTAACTAAACAAGGT+AGG - chr2.2:2267744-2267763 None:intergenic 20.0%
!! ATAAGAGAATAAATTACAGA+TGG + chr2.2:2267947-2267966 MS.gene36354:intron 20.0%
!! TAAACTTGAAATATGTGTTA+AGG + chr2.2:2268370-2268389 MS.gene36354:intron 20.0%
!!! TCAACAATTTTTTTTGAACA+AGG - chr2.2:2267912-2267931 None:intergenic 20.0%
! AACTTTACGAATTAATTAGC+CGG + chr2.2:2268487-2268506 MS.gene36354:intron 25.0%
! TGTTAGAGTACATAATATAC+CGG - chr2.2:2268509-2268528 None:intergenic 25.0%
!! AAGTAACCATTTTGCATTTT+TGG + chr2.2:2267793-2267812 MS.gene36354:intron 25.0%
!! TCAATGAATCTTTTGGATTT+AGG + chr2.2:2268579-2268598 MS.gene36354:intron 25.0%
TAACAAATCGAATCTTTCTG+AGG + chr2.2:2268524-2268543 MS.gene36354:intron 30.0%
TGAAGAAATTGTTGTGATTG+TGG - chr2.2:2267431-2267450 None:intergenic 30.0%
! TTGGATTTAGGATATACTCT+TGG + chr2.2:2268591-2268610 MS.gene36354:intron 30.0%
!! TATGATCCAGAGTTGATATA+CGG + chr2.2:2267830-2267849 MS.gene36354:intron 30.0%
!!! TGATTTTGTTTTTGTGAGCA+CGG - chr2.2:2268201-2268220 None:intergenic 30.0%
AGTACAAAGATGTGTACGAA+GGG - chr2.2:2268552-2268571 None:intergenic 35.0%
ATCAATCTTGCTTCCTCAAT+TGG - chr2.2:2267701-2267720 None:intergenic 35.0%
CTGCTGAAGATTTCATAAAC+AGG + chr2.2:2267667-2267686 MS.gene36354:intron 35.0%
TAGTACAAAGATGTGTACGA+AGG - chr2.2:2268553-2268572 None:intergenic 35.0%
TCTAAGAGAAGATGATGAGA+AGG + chr2.2:2267626-2267645 MS.gene36354:intron 35.0%
TCTCATCATCTTCTCTTAGA+GGG - chr2.2:2267626-2267645 None:intergenic 35.0%
TTACAACCCAAAATTCTGCA+AGG - chr2.2:2268631-2268650 None:intergenic 35.0%
TTCTCATCATCTTCTCTTAG+AGG - chr2.2:2267627-2267646 None:intergenic 35.0%
! ATAAGCACCTTGCAGAATTT+TGG + chr2.2:2268621-2268640 MS.gene36354:intron 35.0%
! GACGGTGATTTTCTTCTTTT+TGG - chr2.2:2268177-2268196 None:intergenic 35.0%
! GGTGATTTTCTTCTTTTTGG+TGG - chr2.2:2268174-2268193 None:intergenic 35.0%
! TAAGCACCTTGCAGAATTTT+GGG + chr2.2:2268622-2268641 MS.gene36354:intron 35.0%
ACTTTACGTACTCTCGCATT+TGG + chr2.2:2268285-2268304 MS.gene36354:intron 40.0%
ATTGTTAAGCATCTCCAGCA+CGG - chr2.2:2267514-2267533 None:intergenic 40.0%
CCCTATTGGGAAAAAGTGAT+GGG - chr2.2:2267388-2267407 None:intergenic 40.0%
CCTATTGGGAAAAAGTGATG+GGG - chr2.2:2267387-2267406 None:intergenic 40.0%
CGTATAAATCTCCCACATGT+AGG - chr2.2:2267858-2267877 None:intergenic 40.0%
GAATTCATACTCCCTTGATG+CGG - chr2.2:2267349-2267368 None:intergenic 40.0%
GAGAAAAAACACAAGAGCAG+CGG - chr2.2:2267994-2268013 None:intergenic 40.0%
GATATACGGTACCTACATGT+GGG + chr2.2:2267844-2267863 MS.gene36354:intron 40.0%
GTGGAACATTAGATTGGCAA+TGG - chr2.2:2267259-2267278 None:intergenic 40.0%
TCATCTTCTCTTAGAGGGAA+TGG - chr2.2:2267621-2267640 None:intergenic 40.0%
TCCCTATTGGGAAAAAGTGA+TGG - chr2.2:2267389-2267408 None:intergenic 40.0%
TGATATACGGTACCTACATG+TGG + chr2.2:2267843-2267862 MS.gene36354:intron 40.0%
! ACAGGTTTTACAGCCAATTG+AGG + chr2.2:2267685-2267704 MS.gene36354:intron 40.0%
! TAGATTGGCAATGGCTTTTC+CGG - chr2.2:2267250-2267269 None:intergenic 40.0%
! TAGGTACCGTATATCAACTC+TGG - chr2.2:2267839-2267858 None:intergenic 40.0%
!! GAAGGACGATCAAGTTGATA+AGG + chr2.2:2267644-2267663 MS.gene36354:intron 40.0%
!! GTGATGGTGGAACATTAGAT+TGG - chr2.2:2267265-2267284 None:intergenic 40.0%
!! TACCAATTGAGTTGAGCTCA+CGG - chr2.2:2268120-2268139 None:intergenic 40.0%
!!! CCCATCACTTTTTCCCAATA+GGG + chr2.2:2267385-2267404 MS.gene36354:CDS 40.0%
AAAAAACACAAGAGCAGCGG+TGG - chr2.2:2267991-2268010 None:intergenic 45.0%
ACATCATCGTCTTGTGTAGC+TGG - chr2.2:2267468-2267487 None:intergenic 45.0%
ACCCTCAATTGCCTTACCAT+TGG - chr2.2:2267588-2267607 None:intergenic 45.0%
ACTCCCTTGATGCGGTAAAT+TGG - chr2.2:2267341-2267360 None:intergenic 45.0%
AGCTACACAAGACGATGATG+TGG + chr2.2:2267467-2267486 MS.gene36354:CDS 45.0%
CCACCAATTTACCGCATCAA+GGG + chr2.2:2267335-2267354 MS.gene36354:CDS 45.0%
CCCAATGGTAAGGCAATTGA+GGG + chr2.2:2267584-2267603 MS.gene36354:intron 45.0%
CCCTCAATTGCCTTACCATT+GGG - chr2.2:2267587-2267606 None:intergenic 45.0%
GGCGTTTGAGCATCATGTTT+AGG - chr2.2:2267215-2267234 None:intergenic 45.0%
TGTGATTGTGGTTGTTGCTG+CGG - chr2.2:2267419-2267438 None:intergenic 45.0%
!! TGTTTTTGTGAGCACGGTGA+CGG - chr2.2:2268195-2268214 None:intergenic 45.0%
!!! CCCCATCACTTTTTCCCAAT+AGG + chr2.2:2267384-2267403 MS.gene36354:CDS 45.0%
!!! TTTTAAATTCTAAATTTTTT+TGG + chr2.2:2268241-2268260 MS.gene36354:intron 5.0%
CACCGTGAGCTCAACTCAAT+TGG + chr2.2:2268115-2268134 MS.gene36354:intron 50.0%
CCCTTGATGCGGTAAATTGG+TGG - chr2.2:2267338-2267357 None:intergenic 50.0%
GCCACCAATTTACCGCATCA+AGG + chr2.2:2267334-2267353 MS.gene36354:CDS 50.0%
GCCCAATGGTAAGGCAATTG+AGG + chr2.2:2267583-2267602 MS.gene36354:intron 50.0%
GGTGACAATGAGCGCAATGA+AGG + chr2.2:2267488-2267507 MS.gene36354:CDS 50.0%
TGCGGTGACGTTTCCCTATT+GGG - chr2.2:2267401-2267420 None:intergenic 50.0%
CTGCGGTGACGTTTCCCTAT+TGG - chr2.2:2267402-2267421 None:intergenic 55.0%
TCTCCAGCACTGCCTGGATT+CGG + chr2.2:2267555-2267574 MS.gene36354:CDS 55.0%
!!! CTTTTCCGGCAAGCTTGCTG+CGG - chr2.2:2267236-2267255 None:intergenic 55.0%
AAACGCCGCAGCAAGCTTGC+CGG + chr2.2:2267228-2267247 MS.gene36354:CDS 60.0%
ATTGGGCTACGCCCGAATCC+AGG - chr2.2:2267570-2267589 None:intergenic 60.0%
CATCACAGCCACCACAGCCA+CGG + chr2.2:2267279-2267298 MS.gene36354:CDS 60.0%
CTCCAGCACTGCCTGGATTC+GGG + chr2.2:2267556-2267575 MS.gene36354:CDS 60.0%
TCGGGCGTAGCCCAATGGTA+AGG + chr2.2:2267574-2267593 MS.gene36354:intron 60.0%
TGAGTCGTGAGAGCGGCTTG+AGG - chr2.2:2267313-2267332 None:intergenic 60.0%
TGGATTCGGGCGTAGCCCAA+TGG + chr2.2:2267569-2267588 MS.gene36354:CDS 60.0%
! GAAGCATCTCCAGCACTGCC+TGG + chr2.2:2267549-2267568 MS.gene36354:CDS 60.0%
CGTGGCTGTGGTGGCTGTGA+TGG - chr2.2:2267281-2267300 None:intergenic 65.0%
GAGCGCAATGAAGGCCGTGC+TGG + chr2.2:2267497-2267516 MS.gene36354:CDS 65.0%
GGCTGTGGTGGCTGTGATGG+TGG - chr2.2:2267278-2267297 None:intergenic 65.0%
GGTGGCGTGAGTCGTGAGAG+CGG - chr2.2:2267320-2267339 None:intergenic 65.0%
GTCGTGAGAGCGGCTTGAGG+TGG - chr2.2:2267310-2267329 None:intergenic 65.0%
CACAGCCACCACAGCCACGG+CGG + chr2.2:2267282-2267301 MS.gene36354:CDS 70.0%
CGCCCGAATCCAGGCAGTGC+TGG - chr2.2:2267561-2267580 None:intergenic 70.0%
AGGTGGAGCCGCCGTGGCTG+TGG - chr2.2:2267293-2267312 None:intergenic 75.0%
GGCTTGAGGTGGAGCCGCCG+TGG - chr2.2:2267299-2267318 None:intergenic 75.0%
TGGAGCCGCCGTGGCTGTGG+TGG - chr2.2:2267290-2267309 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 2267207 2268735 2267207 ID=MS.gene36354
chr2.2 mRNA 2267207 2268735 2267207 ID=MS.gene36354.t1;Parent=MS.gene36354
chr2.2 exon 2267207 2267590 2267207 ID=MS.gene36354.t1.exon1;Parent=MS.gene36354.t1
chr2.2 CDS 2267207 2267590 2267207 ID=cds.MS.gene36354.t1;Parent=MS.gene36354.t1
chr2.2 exon 2268724 2268735 2268724 ID=MS.gene36354.t1.exon2;Parent=MS.gene36354.t1
chr2.2 CDS 2268724 2268735 2268724 ID=cds.MS.gene36354.t1;Parent=MS.gene36354.t1
Gene Sequence

>MS.gene36354

ATGGGCCTAAACATGATGCTCAAACGCCGCAGCAAGCTTGCCGGAAAAGCCATTGCCAATCTAATGTTCCACCATCACAGCCACCACAGCCACGGCGGCTCCACCTCAAGCCGCTCTCACGACTCACGCCACCAATTTACCGCATCAAGGGAGTATGAATTCAGCTGCAGCAACACTCCCCATCACTTTTTCCCAATAGGGAAACGTCACCGCAGCAACAACCACAATCACAACAATTTCTTCACTTGCGCTCATACGCCAGCTACACAAGACGATGATGTGGTGACAATGAGCGCAATGAAGGCCGTGCTGGAGATGCTTAACAATGATCAAGCAATCGTAGAAGCATCTCCAGCACTGCCTGGATTCGGGCGTAGCCCAATGGTAAGGCAATTGAGGGTGACTGACTCACCATTCCCTCTAAGAGAAGATGATGAGAAGGACGATCAAGTTGATAAGGCTGCTGAAGATTTCATAAACAGGTTTTACAGCCAATTGAGGAAGCAAGATTGATTGATTAAATAATTAATTCAACCTACCTTGTTTAGTTAATAATTAATGTGTGGAATCGTTTGTTGTGAATGCTAAGTAACCATTTTGCATTTTTGGTTATTTTCATGATATATGATCCAGAGTTGATATACGGTACCTACATGTGGGAGATTTATACGTCTCATGTTTGATTTAATTGTTGATTATCAACCTTGTTCAAAAAAAATTGTTGATTATCAACAATAATAATAAGAGAATAAATTACAGATGGAATTAATTAATTGATTATCCACCGCTGCTCTTGTGTTTTTTCTCAAATAAATTACGGCATATAATTCCTTTTCATAATTTCTAACAAAATATATAATCTCATGCCTTAAAAAGAATCTCATATATATGTTTGGTGAGTAACTAATCACCGTGAGCTCAACTCAATTGGTAAAAACAATGTATAATATATGTAAATCTCATACCACCAAAAAGAAGAAAATCACCGTCACCGTGCTCACAAAAACAAAATCACCTTTCTTAATAATAATCACTTTTAAATTCTAAATTTTTTTGGTTGTGTACTTTATTTTAGTTAACTTTACGTACTCTCGCATTTGGAGTGCTTTTATTTTGAGTCTATCTATGAATTGAAGAAAAAGACTACACTTTAAATTCGTATATAAACTTGAAATATGTGTTAAGGGTTCAAACATAAGCTTGTTTTTGTTATTATCTTTCATTATTAATAATAGATAGAAAATATTTGTTTTTTAAACAATAATTGAAATATTTTAGTTAACTTTACGAATTAATTAGCCGGTATATTATGTACTCTAACAAATCGAATCTTTCTGAGGTACCCTTCGTACACATCTTTGTACTAAAAATTTCAATGAATCTTTTGGATTTAGGATATACTCTTGGCTATAAGATAAGCACCTTGCAGAATTTTGGGTTGTAACAACAATATTAAACATTTTTTCTTCTCTTTCTAACTAGTTTCTTCATTTTAATTCTTGCTGTTAACCTCTTAGCCTTTGCCTTAA

Protein sequence

>MS.gene36354.t1

MGLNMMLKRRSKLAGKAIANLMFHHHSHHSHGGSTSSRSHDSRHQFTASREYEFSCSNTPHHFFPIGKRHRSNNHNHNNFFTCAHTPATQDDDVVTMSAMKAVLEMLNNDQAIVEASPALPGFGRSPMPLP