AlfalfaGEDB | MS.gene72413

M. sativa cultivar XinJiangDaYe / MS.gene72413

KEGG: K14847
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72413.t1	XP_013468062.1	100	101	0	0	1	101	241	341	1.70E-44	188.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72413.t1	Q9LUG5	65.0	100	33	2	1	100	216	313	4.1e-23	108.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72413.t1	I3SPQ7	100.0	101	0	0	1	101	241	341	1.2e-44	188.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049129	MS.gene72413	-0.834956	2.22E-56	-1.69E-46
MS.gene04918	MS.gene72413	0.815519	8.87E-52	-1.69E-46
MS.gene049255	MS.gene72413	0.815063	1.12E-51	-1.69E-46
MS.gene049368	MS.gene72413	0.809359	1.97E-50	-1.69E-46
MS.gene049411	MS.gene72413	0.875811	2.29E-68	-1.69E-46
MS.gene049808	MS.gene72413	0.821312	4.32E-53	-1.69E-46
MS.gene049825	MS.gene72413	0.802416	5.67E-49	-1.69E-46
MS.gene049873	MS.gene72413	0.807472	4.97E-50	-1.69E-46
MS.gene050437	MS.gene72413	0.821155	4.69E-53	-1.69E-46
MS.gene050689	MS.gene72413	0.836238	1.05E-56	-1.69E-46
MS.gene050752	MS.gene72413	0.851732	7.33E-61	-1.69E-46
MS.gene050838	MS.gene72413	0.834143	3.55E-56	-1.69E-46
MS.gene050945	MS.gene72413	0.805322	1.41E-49	-1.69E-46
MS.gene050955	MS.gene72413	0.852125	5.67E-61	-1.69E-46
MS.gene050985	MS.gene72413	0.836036	1.18E-56	-1.69E-46
MS.gene051003	MS.gene72413	0.862291	5.62E-64	-1.69E-46
MS.gene051234	MS.gene72413	0.864635	1.06E-64	-1.69E-46
MS.gene051328	MS.gene72413	0.813205	2.88E-51	-1.69E-46
MS.gene051343	MS.gene72413	0.812345	4.44E-51	-1.69E-46
MS.gene05134	MS.gene72413	0.859127	5.13E-63	-1.69E-46
MS.gene051363	MS.gene72413	0.809677	1.68E-50	-1.69E-46
MS.gene051365	MS.gene72413	0.817241	3.65E-52	-1.69E-46
MS.gene05188	MS.gene72413	0.821959	3.06E-53	-1.69E-46
MS.gene051902	MS.gene72413	0.827242	1.74E-54	-1.69E-46
MS.gene051975	MS.gene72413	0.813263	2.80E-51	-1.69E-46
MS.gene052072	MS.gene72413	0.850797	1.35E-60	-1.69E-46
MS.gene052296	MS.gene72413	0.802495	5.46E-49	-1.69E-46
MS.gene052335	MS.gene72413	0.80826	3.38E-50	-1.69E-46
MS.gene052342	MS.gene72413	0.804639	1.96E-49	-1.69E-46
MS.gene052479	MS.gene72413	0.831791	1.37E-55	-1.69E-46
MS.gene052516	MS.gene72413	0.800608	1.33E-48	-1.69E-46
MS.gene052518	MS.gene72413	0.819233	1.29E-52	-1.69E-46
MS.gene052519	MS.gene72413	0.818156	2.27E-52	-1.69E-46
MS.gene052947	MS.gene72413	0.86279	3.95E-64	-1.69E-46
MS.gene053122	MS.gene72413	0.826229	3.04E-54	-1.69E-46
MS.gene053226	MS.gene72413	0.800346	1.50E-48	-1.69E-46
MS.gene05364	MS.gene72413	0.850517	1.61E-60	-1.69E-46
MS.gene053931	MS.gene72413	0.807662	4.53E-50	-1.69E-46
MS.gene054004	MS.gene72413	0.823909	1.07E-53	-1.69E-46
MS.gene054142	MS.gene72413	0.818685	1.72E-52	-1.69E-46
MS.gene054403	MS.gene72413	0.85466	1.06E-61	-1.69E-46
MS.gene055162	MS.gene72413	0.821837	3.26E-53	-1.69E-46
MS.gene05517	MS.gene72413	0.806398	8.39E-50	-1.69E-46
MS.gene055468	MS.gene72413	0.802801	4.72E-49	-1.69E-46
MS.gene055555	MS.gene72413	0.874972	4.44E-68	-1.69E-46
MS.gene055580	MS.gene72413	-0.802186	6.32E-49	-1.69E-46
MS.gene055765	MS.gene72413	0.821722	3.47E-53	-1.69E-46
MS.gene055979	MS.gene72413	0.801697	7.97E-49	-1.69E-46
MS.gene056043	MS.gene72413	-0.803017	4.26E-49	-1.69E-46
MS.gene056075	MS.gene72413	0.815222	1.03E-51	-1.69E-46
MS.gene056215	MS.gene72413	0.825947	3.55E-54	-1.69E-46
MS.gene05648	MS.gene72413	0.815204	1.04E-51	-1.69E-46
MS.gene056986	MS.gene72413	0.811668	6.24E-51	-1.69E-46
MS.gene057102	MS.gene72413	0.859649	3.57E-63	-1.69E-46
MS.gene057694	MS.gene72413	0.842887	1.97E-58	-1.69E-46
MS.gene057705	MS.gene72413	0.878042	3.87E-69	-1.69E-46
MS.gene05781	MS.gene72413	0.806313	8.74E-50	-1.69E-46
MS.gene058008	MS.gene72413	0.80617	9.37E-50	-1.69E-46
MS.gene058009	MS.gene72413	0.801337	9.44E-49	-1.69E-46
MS.gene058149	MS.gene72413	0.809908	1.50E-50	-1.69E-46
MS.gene058781	MS.gene72413	0.801886	7.29E-49	-1.69E-46
MS.gene058783	MS.gene72413	0.832388	9.73E-56	-1.69E-46
MS.gene059146	MS.gene72413	0.858367	8.65E-63	-1.69E-46
MS.gene059151	MS.gene72413	0.808935	2.42E-50	-1.69E-46
MS.gene059227	MS.gene72413	0.841787	3.84E-58	-1.69E-46
MS.gene059802	MS.gene72413	0.807821	4.19E-50	-1.69E-46
MS.gene05985	MS.gene72413	-0.825321	5.00E-54	-1.69E-46
MS.gene06011	MS.gene72413	0.820553	6.46E-53	-1.69E-46
MS.gene060244	MS.gene72413	0.808199	3.48E-50	-1.69E-46
MS.gene060365	MS.gene72413	0.802129	6.50E-49	-1.69E-46
MS.gene060429	MS.gene72413	0.821062	4.93E-53	-1.69E-46
MS.gene060763	MS.gene72413	0.855102	7.90E-62	-1.69E-46
MS.gene060917	MS.gene72413	0.840787	7.05E-58	-1.69E-46
MS.gene060935	MS.gene72413	0.837594	4.74E-57	-1.69E-46
MS.gene060937	MS.gene72413	0.826211	3.07E-54	-1.69E-46
MS.gene061100	MS.gene72413	0.837642	4.61E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72413.t1	MTR_1g061160	100.000	101	0	0	1	101	241	341	3.31e-67	205

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72413.t1	AT3G23620	65.000	100	33	2	1	100	216	313	4.09e-28	103

Find 28 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTCTGCTGCTTGGCTTCT+CGG	0.282208	1.4:-50357783	None:intergenic
ATTTCCAATCTTTCCTTGTA+AGG	0.319160	1.4:-50357590	None:intergenic
AGTTGGTCCTTCCATGGATA+TGG	0.326164	1.4:+50357375	MS.gene72413:CDS
AAGGGACAAGCCAAAGAAAA+AGG	0.337552	1.4:+50357456	MS.gene72413:CDS
ACTCCCATGTTTCTGCTGCT+TGG	0.361871	1.4:-50357791	None:intergenic
AATTGTTTAGATTGGAGAAA+TGG	0.368193	1.4:+50357723	MS.gene72413:intron
TTCACTCCTTTCGATTTATT+AGG	0.370859	1.4:-50357751	None:intergenic
ATAGGAGAAGAATGTCAAAA+AGG	0.389857	1.4:+50357564	MS.gene72413:intron
TCCTTCATCACACTTTCATT+AGG	0.397326	1.4:-50357418	None:intergenic
GTCCCTCTTACCTTCTGATC+TGG	0.404383	1.4:-50357622	None:intergenic
GATCCCTTACAAGGAAAGAT+TGG	0.422525	1.4:+50357586	MS.gene72413:CDS
AGTTGTCCCAAGAATGGAAT+TGG	0.444927	1.4:+50357348	None:intergenic
TTCATCACACTTTCATTAGG+AGG	0.486397	1.4:-50357415	None:intergenic
TCCTAATGAAAGTGTGATGA+AGG	0.493736	1.4:+50357417	MS.gene72413:CDS
GAAGCCAAGCAGCAGAAACA+TGG	0.494056	1.4:+50357787	MS.gene72413:CDS
TTTCCAATCTTTCCTTGTAA+GGG	0.502446	1.4:-50357589	None:intergenic
ATAAATCGAAAGGAGTGAAG+AGG	0.524729	1.4:+50357755	MS.gene72413:CDS
GGTAGAAGTTGGTCCTTCCA+TGG	0.530363	1.4:+50357369	MS.gene72413:CDS
AGAATGGAATTGGTAGAAGT+TGG	0.538211	1.4:+50357358	None:intergenic
TTTCATTAGGAGGACGATGC+CGG	0.551349	1.4:-50357405	None:intergenic
CCGGCGAATAACCATATCCA+TGG	0.560635	1.4:-50357386	None:intergenic
AGGAAGCCATGAAAACTTCA+AGG	0.562762	1.4:+50357437	MS.gene72413:CDS
CCATGGATATGGTTATTCGC+CGG	0.570411	1.4:+50357386	MS.gene72413:CDS
TGTTTATATTCCAGATCAGA+AGG	0.574269	1.4:+50357612	MS.gene72413:CDS
AAGCCAAGCAGCAGAAACAT+GGG	0.578219	1.4:+50357788	MS.gene72413:CDS
TAAATCGAAAGGAGTGAAGA+GGG	0.586006	1.4:+50357756	MS.gene72413:CDS
CGAATAACCATATCCATGGA+AGG	0.616987	1.4:-50357382	None:intergenic
GGAAGCCATGAAAACTTCAA+GGG	0.623284	1.4:+50357438	MS.gene72413:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCGATTTTAATTGTTTAGAT+TGG	+	chr1.4:50357715-50357734	MS.gene72413:intron	20.0%
!	AATTGTTTAGATTGGAGAAA+TGG	+	chr1.4:50357723-50357742	MS.gene72413:intron	25.0%
!	ACTTGAAAGATTTAGAGAAT+TGG	-	chr1.4:50357486-50357505	None:intergenic	25.0%
!!!	AATTGGTTTACCTTTTTCTT+TGG	-	chr1.4:50357469-50357488	None:intergenic	25.0%
	ATAGGAGAAGAATGTCAAAA+AGG	+	chr1.4:50357564-50357583	MS.gene72413:intron	30.0%
	ATTTCCAATCTTTCCTTGTA+AGG	-	chr1.4:50357593-50357612	None:intergenic	30.0%
	TGTTTATATTCCAGATCAGA+AGG	+	chr1.4:50357612-50357631	MS.gene72413:CDS	30.0%
	TTCACTCCTTTCGATTTATT+AGG	-	chr1.4:50357754-50357773	None:intergenic	30.0%
	TTTCCAATCTTTCCTTGTAA+GGG	-	chr1.4:50357592-50357611	None:intergenic	30.0%
!	GTTTTGCCTAATAAATCGAA+AGG	+	chr1.4:50357745-50357764	MS.gene72413:CDS	30.0%
!!	GTACTTGTTCTGTTTCTAAT+AGG	+	chr1.4:50357546-50357565	MS.gene72413:intron	30.0%
	ATAAATCGAAAGGAGTGAAG+AGG	+	chr1.4:50357755-50357774	MS.gene72413:CDS	35.0%
	TAAATCGAAAGGAGTGAAGA+GGG	+	chr1.4:50357756-50357775	MS.gene72413:CDS	35.0%
	TCCTTCATCACACTTTCATT+AGG	-	chr1.4:50357421-50357440	None:intergenic	35.0%
	TTCATCACACTTTCATTAGG+AGG	-	chr1.4:50357418-50357437	None:intergenic	35.0%
!	TCCTAATGAAAGTGTGATGA+AGG	+	chr1.4:50357417-50357436	MS.gene72413:CDS	35.0%
	AAGGGACAAGCCAAAGAAAA+AGG	+	chr1.4:50357456-50357475	MS.gene72413:CDS	40.0%
	AGGAAGCCATGAAAACTTCA+AGG	+	chr1.4:50357437-50357456	MS.gene72413:CDS	40.0%
	ATTCCAGATCAGAAGGTAAG+AGG	+	chr1.4:50357619-50357638	MS.gene72413:intron	40.0%
	CGAATAACCATATCCATGGA+AGG	-	chr1.4:50357385-50357404	None:intergenic	40.0%
	GATCCCTTACAAGGAAAGAT+TGG	+	chr1.4:50357586-50357605	MS.gene72413:CDS	40.0%
	GGAAGCCATGAAAACTTCAA+GGG	+	chr1.4:50357438-50357457	MS.gene72413:CDS	40.0%
	GTCAAAAAGGATCCCTTACA+AGG	+	chr1.4:50357577-50357596	MS.gene72413:CDS	40.0%
	TTCCAGATCAGAAGGTAAGA+GGG	+	chr1.4:50357620-50357639	MS.gene72413:intron	40.0%
!	CTTGTCCCTTGAAGTTTTCA+TGG	-	chr1.4:50357446-50357465	None:intergenic	40.0%
	AAGCCAAGCAGCAGAAACAT+GGG	+	chr1.4:50357788-50357807	MS.gene72413:CDS	45.0%
	AGTTGGTCCTTCCATGGATA+TGG	+	chr1.4:50357375-50357394	MS.gene72413:CDS	45.0%
	CCATGGATATGGTTATTCGC+CGG	+	chr1.4:50357386-50357405	MS.gene72413:CDS	45.0%
	GCATGCATCGAAAAAGCAGA+AGG	+	chr1.4:50357819-50357838	MS.gene72413:CDS	45.0%
	TTTCATTAGGAGGACGATGC+CGG	-	chr1.4:50357408-50357427	None:intergenic	45.0%
	CCGGCGAATAACCATATCCA+TGG	-	chr1.4:50357389-50357408	None:intergenic	50.0%
	GAAGCCAAGCAGCAGAAACA+TGG	+	chr1.4:50357787-50357806	MS.gene72413:CDS	50.0%
	GTCCCTCTTACCTTCTGATC+TGG	-	chr1.4:50357625-50357644	None:intergenic	50.0%
!	ACTCCCATGTTTCTGCTGCT+TGG	-	chr1.4:50357794-50357813	None:intergenic	50.0%
!	GGTAGAAGTTGGTCCTTCCA+TGG	+	chr1.4:50357369-50357388	MS.gene72413:CDS	50.0%
!	GTTTCTGCTGCTTGGCTTCT+CGG	-	chr1.4:50357786-50357805	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	50357361	50357855	50357361	ID=MS.gene72413
chr1.4	mRNA	50357361	50357855	50357361	ID=MS.gene72413.t1;Parent=MS.gene72413
chr1.4	exon	50357361	50357477	50357361	ID=MS.gene72413.t1.exon1;Parent=MS.gene72413.t1
chr1.4	CDS	50357361	50357477	50357361	ID=cds.MS.gene72413.t1;Parent=MS.gene72413.t1
chr1.4	exon	50357568	50357633	50357568	ID=MS.gene72413.t1.exon2;Parent=MS.gene72413.t1
chr1.4	CDS	50357568	50357633	50357568	ID=cds.MS.gene72413.t1;Parent=MS.gene72413.t1
chr1.4	exon	50357733	50357855	50357733	ID=MS.gene72413.t1.exon3;Parent=MS.gene72413.t1
chr1.4	CDS	50357733	50357855	50357733	ID=cds.MS.gene72413.t1;Parent=MS.gene72413.t1

Gene Sequence

>MS.gene72413

ATGGAATTGGTAGAAGTTGGTCCTTCCATGGATATGGTTATTCGCCGGCATCGTCCTCCTAATGAAAGTGTGATGAAGGAAGCCATGAAAACTTCAAGGGACAAGCCAAAGAAAAAGGTAAACCAATTCTCTAAATCTTTCAAGTTTATCTGTTAATGTAAAATGAAGTTGCATGTCTAAATCGTGTACTTGTTCTGTTTCTAATAGGAGAAGAATGTCAAAAAGGATCCCTTACAAGGAAAGATTGGAAATGTTTATATTCCAGATCAGAAGGTAAGAGGGACTATTCTTGTTCTTTGCTTTAACTATTTTAGTTAGTTCATTCAACTTAGTACATTGAATTTGCTTATGATATCGATTTTAATTGTTTAGATTGGAGAAATGGTTTTGCCTAATAAATCGAAAGGAGTGAAGAGGGAGCGCCGAGAAGCCAAGCAGCAGAAACATGGGAGTGATGAGCATGCATCGAAAAAGCAGAAGGATGAATCTTCCTAA

Protein sequence

>MS.gene72413.t1

MELVEVGPSMDMVIRRHRPPNESVMKEAMKTSRDKPKKKEKNVKKDPLQGKIGNVYIPDQKIGEMVLPNKSKGVKRERREAKQQKHGSDEHASKKQKDESS