Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77769.t1 | XP_003628200.1 | 86.5 | 156 | 21 | 0 | 1 | 156 | 1 | 156 | 1.10E-77 | 299.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77769.t1 | Q9SSK5 | 33.3 | 153 | 99 | 3 | 3 | 153 | 7 | 158 | 9.5e-19 | 94.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77769.t1 | G7L7T5 | 86.5 | 156 | 21 | 0 | 1 | 156 | 1 | 156 | 7.6e-78 | 299.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77769.t1 | MTR_8g045490 | 86.538 | 156 | 21 | 0 | 1 | 156 | 1 | 156 | 7.58e-101 | 286 |
| MS.gene77769.t1 | MTR_8g045520 | 77.707 | 157 | 34 | 1 | 1 | 156 | 1 | 157 | 6.23e-89 | 256 |
| MS.gene77769.t1 | MTR_8g045400 | 75.497 | 151 | 36 | 1 | 1 | 151 | 1 | 150 | 1.63e-84 | 245 |
| MS.gene77769.t1 | MTR_8g045570 | 74.834 | 151 | 38 | 0 | 1 | 151 | 1 | 151 | 2.88e-84 | 244 |
| MS.gene77769.t1 | MTR_8g045695 | 71.975 | 157 | 42 | 2 | 1 | 156 | 1 | 156 | 8.19e-82 | 238 |
| MS.gene77769.t1 | MTR_8g045300 | 71.711 | 152 | 43 | 0 | 1 | 152 | 1 | 152 | 1.73e-80 | 235 |
| MS.gene77769.t1 | MTR_8g045555 | 73.510 | 151 | 40 | 0 | 1 | 151 | 1 | 151 | 4.93e-80 | 233 |
| MS.gene77769.t1 | MTR_8g045735 | 70.199 | 151 | 44 | 1 | 1 | 151 | 1 | 150 | 1.01e-77 | 228 |
| MS.gene77769.t1 | MTR_8g045665 | 69.536 | 151 | 45 | 1 | 1 | 151 | 1 | 150 | 1.47e-75 | 222 |
| MS.gene77769.t1 | MTR_8g045640 | 68.153 | 157 | 48 | 2 | 1 | 156 | 1 | 156 | 1.75e-75 | 222 |
| MS.gene77769.t1 | MTR_8g045560 | 67.550 | 151 | 48 | 1 | 1 | 151 | 1 | 150 | 4.34e-73 | 216 |
| MS.gene77769.t1 | MTR_1g030810 | 44.156 | 154 | 83 | 2 | 1 | 152 | 1 | 153 | 2.27e-39 | 130 |
| MS.gene77769.t1 | MTR_1g030820 | 39.610 | 154 | 90 | 2 | 1 | 152 | 1 | 153 | 8.54e-38 | 126 |
| MS.gene77769.t1 | MTR_1g030840 | 36.478 | 159 | 93 | 4 | 1 | 152 | 1 | 158 | 4.81e-26 | 97.1 |
| MS.gene77769.t1 | MTR_8g012550 | 32.432 | 148 | 97 | 3 | 6 | 152 | 8 | 153 | 2.21e-25 | 95.1 |
| MS.gene77769.t1 | MTR_1g031640 | 36.054 | 147 | 85 | 4 | 7 | 151 | 7 | 146 | 7.28e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77769.t1 | AT5G28010 | 33.333 | 153 | 99 | 3 | 3 | 153 | 15 | 166 | 8.08e-24 | 91.7 |
| MS.gene77769.t1 | AT5G28010 | 33.333 | 153 | 99 | 3 | 3 | 153 | 15 | 166 | 8.08e-24 | 91.7 |
| MS.gene77769.t1 | AT1G70890 | 33.333 | 153 | 99 | 3 | 3 | 153 | 7 | 158 | 1.23e-21 | 85.9 |
| MS.gene77769.t1 | AT1G70840 | 30.921 | 152 | 102 | 3 | 3 | 152 | 20 | 170 | 5.17e-21 | 84.3 |
| MS.gene77769.t1 | AT5G28000 | 30.921 | 152 | 102 | 3 | 3 | 152 | 13 | 163 | 1.46e-20 | 83.2 |
| MS.gene77769.t1 | AT1G70830 | 31.373 | 153 | 102 | 3 | 3 | 153 | 22 | 173 | 3.03e-20 | 82.4 |
| MS.gene77769.t1 | AT1G23120 | 29.605 | 152 | 102 | 3 | 1 | 152 | 1 | 147 | 3.75e-20 | 81.6 |
| MS.gene77769.t1 | AT1G70830 | 31.373 | 153 | 102 | 3 | 3 | 153 | 22 | 173 | 5.70e-20 | 82.4 |
| MS.gene77769.t1 | AT1G23120 | 29.605 | 152 | 102 | 3 | 1 | 152 | 51 | 197 | 1.60e-19 | 81.3 |
| MS.gene77769.t1 | AT1G70870 | 44.792 | 96 | 52 | 1 | 56 | 151 | 44 | 138 | 1.91e-19 | 79.3 |
| MS.gene77769.t1 | AT1G70830 | 31.373 | 153 | 102 | 3 | 3 | 153 | 22 | 173 | 2.03e-19 | 82.0 |
| MS.gene77769.t1 | AT1G23130 | 29.801 | 151 | 104 | 2 | 3 | 152 | 10 | 159 | 2.60e-19 | 79.7 |
| MS.gene77769.t1 | AT1G70830 | 31.373 | 153 | 102 | 3 | 3 | 153 | 22 | 173 | 3.75e-19 | 82.4 |
| MS.gene77769.t1 | AT1G70830 | 30.065 | 153 | 104 | 3 | 3 | 153 | 184 | 335 | 1.32e-17 | 78.2 |
| MS.gene77769.t1 | AT4G23670 | 33.553 | 152 | 98 | 3 | 1 | 151 | 1 | 150 | 5.15e-18 | 76.3 |
| MS.gene77769.t1 | AT1G70830 | 30.065 | 153 | 104 | 3 | 3 | 153 | 137 | 288 | 6.45e-18 | 78.6 |
| MS.gene77769.t1 | AT1G70850 | 29.530 | 149 | 102 | 3 | 3 | 149 | 9 | 156 | 2.12e-17 | 76.3 |
| MS.gene77769.t1 | AT1G35260 | 30.137 | 146 | 98 | 3 | 6 | 149 | 5 | 148 | 4.18e-17 | 73.9 |
| MS.gene77769.t1 | AT1G70850 | 29.530 | 149 | 102 | 3 | 3 | 149 | 9 | 156 | 4.73e-17 | 76.6 |
| MS.gene77769.t1 | AT1G70850 | 30.769 | 143 | 96 | 3 | 13 | 153 | 175 | 316 | 9.70e-17 | 75.9 |
| MS.gene77769.t1 | AT1G70850 | 29.530 | 149 | 102 | 3 | 3 | 149 | 9 | 156 | 4.73e-17 | 76.6 |
| MS.gene77769.t1 | AT1G70850 | 30.769 | 143 | 96 | 3 | 13 | 153 | 175 | 316 | 9.70e-17 | 75.9 |
| MS.gene77769.t1 | AT1G70880 | 28.105 | 153 | 107 | 3 | 3 | 153 | 8 | 159 | 9.95e-17 | 73.2 |
| MS.gene77769.t1 | AT4G23680 | 30.263 | 152 | 103 | 3 | 1 | 151 | 1 | 150 | 1.28e-15 | 70.1 |
| MS.gene77769.t1 | AT2G01520 | 29.605 | 152 | 104 | 3 | 1 | 151 | 1 | 150 | 3.56e-15 | 68.9 |
| MS.gene77769.t1 | AT1G14930 | 30.818 | 159 | 93 | 5 | 1 | 151 | 1 | 150 | 1.38e-14 | 67.4 |
| MS.gene77769.t1 | AT1G35310 | 27.660 | 141 | 99 | 3 | 13 | 152 | 12 | 150 | 1.96e-14 | 66.6 |
| MS.gene77769.t1 | AT1G24020 | 30.769 | 156 | 106 | 2 | 1 | 155 | 1 | 155 | 2.42e-14 | 66.6 |
| MS.gene77769.t1 | AT1G24020 | 30.769 | 156 | 106 | 2 | 1 | 155 | 1 | 155 | 2.42e-14 | 66.6 |
| MS.gene77769.t1 | AT4G14060 | 30.263 | 152 | 103 | 3 | 1 | 151 | 1 | 150 | 2.93e-14 | 66.2 |
| MS.gene77769.t1 | AT2G01530 | 29.605 | 152 | 104 | 3 | 1 | 151 | 1 | 150 | 1.20e-13 | 64.7 |
| MS.gene77769.t1 | AT1G30990 | 30.370 | 135 | 91 | 3 | 1 | 134 | 1 | 133 | 4.86e-12 | 60.5 |
| MS.gene77769.t1 | AT1G14940 | 28.667 | 150 | 104 | 3 | 1 | 149 | 1 | 148 | 1.08e-11 | 59.7 |
| MS.gene77769.t1 | AT1G14950 | 26.623 | 154 | 110 | 3 | 1 | 153 | 1 | 152 | 3.14e-11 | 58.5 |
Find 23 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGGCTACATGGAACATTT+TGG | 0.264766 | 8.1:-59896233 | MS.gene77769:CDS |
| ATCTTCACCTTCATGAAGTT+TGG | 0.293563 | 8.1:+59897639 | None:intergenic |
| ATGGCACTGCTGCTGCTAAA+TGG | 0.314913 | 8.1:-59896298 | MS.gene77769:CDS |
| GGTGAGCTCAGTACTGAAAT+TGG | 0.365883 | 8.1:-59897748 | MS.gene77769:CDS |
| AAATGTTCCATGTAGCCATT+TGG | 0.413070 | 8.1:+59896236 | None:intergenic |
| AACTTCATGAAGGTGAAGAT+TGG | 0.483425 | 8.1:-59897636 | MS.gene77769:CDS |
| AAACACTGGACTTATGTCAT+AGG | 0.490184 | 8.1:-59897595 | MS.gene77769:intron |
| AGATATTGATGAGCACTATA+AGG | 0.500590 | 8.1:-59896357 | MS.gene77769:CDS |
| TGACCCTCCAAATGGCTACA+TGG | 0.521176 | 8.1:-59896243 | MS.gene77769:CDS |
| GAGGATATTGACCCTCCAAA+TGG | 0.540200 | 8.1:-59896251 | MS.gene77769:CDS |
| TGTTCCATGTAGCCATTTGG+AGG | 0.542543 | 8.1:+59896239 | None:intergenic |
| AACACTTATAAGTTCTTCGG+TGG | 0.555536 | 8.1:-59896380 | MS.gene77769:CDS |
| TATGATGAACTCTTTCACAA+AGG | 0.564152 | 8.1:+59897662 | None:intergenic |
| GAAGTAATTGATAAGAGTGA+TGG | 0.579637 | 8.1:-59896317 | MS.gene77769:CDS |
| ATAATTCATAACAGATGGCA+AGG | 0.593174 | 8.1:-59896453 | MS.gene77769:intron |
| CATCATACCAAACTTCATGA+AGG | 0.595182 | 8.1:-59897646 | MS.gene77769:CDS |
| TATTGATGCTTATCTAGCCA+AGG | 0.607429 | 8.1:-59896195 | MS.gene77769:CDS |
| GTTCCATGTAGCCATTTGGA+GGG | 0.622662 | 8.1:+59896240 | None:intergenic |
| ACAGTGATTCAGTTAAACAC+TGG | 0.626084 | 8.1:-59897609 | MS.gene77769:CDS |
| CAGATTGTAGAACTTGTCAG+AGG | 0.636689 | 8.1:+59897714 | None:intergenic |
| TGAATATGAAAAGATCAATG+AGG | 0.646793 | 8.1:-59896270 | MS.gene77769:CDS |
| AGATTGTAGAACTTGTCAGA+GGG | 0.675294 | 8.1:+59897715 | None:intergenic |
| TTCTAAGAAATGGTGCTCTG+TGG | 0.703436 | 8.1:-59897769 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTTGTCAAAAAAAAAAA+GGG | + | chr8.1:59897039-59897058 | None:intergenic | 10.0% |
| !! | TATATTTGTCAAAAAAAAAA+AGG | + | chr8.1:59897040-59897059 | None:intergenic | 10.0% |
| !!! | CTTTTAGATTTTTTTATTTT+TGG | + | chr8.1:59896675-59896694 | None:intergenic | 10.0% |
| !!! | TTATTATTCAATTTTTTGTT+TGG | - | chr8.1:59897089-59897108 | MS.gene77769:intron | 10.0% |
| !! | AGTAAAATAATAAGATGATT+TGG | + | chr8.1:59896401-59896420 | None:intergenic | 15.0% |
| !!! | AAATATTTTAAGGTAAACAA+CGG | + | chr8.1:59897071-59897090 | None:intergenic | 15.0% |
| !!! | AAATGTTGATCATTTTTAAT+TGG | - | chr8.1:59896466-59896485 | MS.gene77769:intron | 15.0% |
| !!! | AATCATCTTATTATTTTACT+TGG | - | chr8.1:59896401-59896420 | MS.gene77769:CDS | 15.0% |
| !!! | GATTCATATTTTTTTTTGAA+TGG | + | chr8.1:59896953-59896972 | None:intergenic | 15.0% |
| !!! | TAGCAAGTTTTATTTTTTTT+TGG | - | chr8.1:59896729-59896748 | MS.gene77769:intron | 15.0% |
| !! | AAAAACACTTATAAGTTCTT+CGG | - | chr8.1:59897555-59897574 | MS.gene77769:intron | 20.0% |
| !! | AGGTTATTTGTAGAAAAAAA+AGG | - | chr8.1:59897378-59897397 | MS.gene77769:intron | 20.0% |
| !!! | AAATAATAAGATGATTTGGT+TGG | + | chr8.1:59896397-59896416 | None:intergenic | 20.0% |
| !!! | TTTTATTTGAATGTTTGCTT+TGG | - | chr8.1:59896549-59896568 | MS.gene77769:intron | 20.0% |
| !!! | TTTTATTTTTTTTTGGTGAC+CGG | - | chr8.1:59896736-59896755 | MS.gene77769:intron | 20.0% |
| ! | TGAATATGAAAAGATCAATG+AGG | - | chr8.1:59897668-59897687 | MS.gene77769:CDS | 25.0% |
| !! | TTGGTCTATATAAAGAGTTT+TGG | + | chr8.1:59896656-59896675 | None:intergenic | 25.0% |
| !!! | TTTATTTTTTTTTGGTGACC+GGG | - | chr8.1:59896737-59896756 | MS.gene77769:intron | 25.0% |
| AACCATACTACTAGACTATT+AGG | + | chr8.1:59897306-59897325 | None:intergenic | 30.0% | |
| AGATATTGATGAGCACTATA+AGG | - | chr8.1:59897581-59897600 | MS.gene77769:intron | 30.0% | |
| ATAATTCATAACAGATGGCA+AGG | - | chr8.1:59897485-59897504 | MS.gene77769:intron | 30.0% | |
| CCATCATAATTCATAACAGA+TGG | - | chr8.1:59897480-59897499 | MS.gene77769:intron | 30.0% | |
| CCATCTGTTATGAATTATGA+TGG | + | chr8.1:59897483-59897502 | None:intergenic | 30.0% | |
| GAAGTAATTGATAAGAGTGA+TGG | - | chr8.1:59897621-59897640 | MS.gene77769:CDS | 30.0% | |
| TAGAGACAGACAATAACTTA+TGG | - | chr8.1:59897274-59897293 | MS.gene77769:intron | 30.0% | |
| TATGATGAACTCTTTCACAA+AGG | + | chr8.1:59896279-59896298 | None:intergenic | 30.0% | |
| TGTTATGAATTATGATGGCA+CGG | + | chr8.1:59897478-59897497 | None:intergenic | 30.0% | |
| ! | TCATTAACCTATTTGCTACA+CGG | - | chr8.1:59897325-59897344 | MS.gene77769:intron | 30.0% |
| ! | TTATAGAGCTATCTTTTCCT+TGG | + | chr8.1:59897763-59897782 | None:intergenic | 30.0% |
| !!! | TTATTTTTTTTTGGTGACCG+GGG | - | chr8.1:59896738-59896757 | MS.gene77769:intron | 30.0% |
| AAACACTGGACTTATGTCAT+AGG | - | chr8.1:59896343-59896362 | MS.gene77769:CDS | 35.0% | |
| AAATGTTCCATGTAGCCATT+TGG | + | chr8.1:59897705-59897724 | None:intergenic | 35.0% | |
| AACACTTATAAGTTCTTCGG+TGG | - | chr8.1:59897558-59897577 | MS.gene77769:intron | 35.0% | |
| AACTTCATGAAGGTGAAGAT+TGG | - | chr8.1:59896302-59896321 | MS.gene77769:CDS | 35.0% | |
| AATAGAGAGAAGAAGAGAGA+AGG | - | chr8.1:59897180-59897199 | MS.gene77769:intron | 35.0% | |
| ACAGTGATTCAGTTAAACAC+TGG | - | chr8.1:59896329-59896348 | MS.gene77769:CDS | 35.0% | |
| AGATTGTAGAACTTGTCAGA+GGG | + | chr8.1:59896226-59896245 | None:intergenic | 35.0% | |
| AGCCTAATAGTCTAGTAGTA+TGG | - | chr8.1:59897301-59897320 | MS.gene77769:intron | 35.0% | |
| ATCTTCACCTTCATGAAGTT+TGG | + | chr8.1:59896302-59896321 | None:intergenic | 35.0% | |
| CATCATACCAAACTTCATGA+AGG | - | chr8.1:59896292-59896311 | MS.gene77769:CDS | 35.0% | |
| GTTATGAATTATGATGGCAC+GGG | + | chr8.1:59897477-59897496 | None:intergenic | 35.0% | |
| TATTGATGCTTATCTAGCCA+AGG | - | chr8.1:59897743-59897762 | MS.gene77769:CDS | 35.0% | |
| ! | AATGGCTACATGGAACATTT+TGG | - | chr8.1:59897705-59897724 | MS.gene77769:CDS | 35.0% |
| ! | TAACATTTTGCCTATGTAGG+TGG | + | chr8.1:59896633-59896652 | None:intergenic | 35.0% |
| ! | TGGTAACATTTTGCCTATGT+AGG | + | chr8.1:59896636-59896655 | None:intergenic | 35.0% |
| AAGGAGAGGAAGAGAGAATA+GGG | - | chr8.1:59897199-59897218 | MS.gene77769:intron | 40.0% | |
| ACACAAAAGACCACCTACAT+AGG | - | chr8.1:59896620-59896639 | MS.gene77769:intron | 40.0% | |
| CAGATTGTAGAACTTGTCAG+AGG | + | chr8.1:59896227-59896246 | None:intergenic | 40.0% | |
| ! | TGAGCTTAACTCAGTTGGTA+GGG | + | chr8.1:59896774-59896793 | None:intergenic | 40.0% |
| AGAGAAGAAGAGAGAAGGAG+AGG | - | chr8.1:59897185-59897204 | MS.gene77769:intron | 45.0% | |
| CCAACTGAGTTAAGCTCACA+AGG | - | chr8.1:59896776-59896795 | MS.gene77769:intron | 45.0% | |
| CCTTGTGAGCTTAACTCAGT+TGG | + | chr8.1:59896779-59896798 | None:intergenic | 45.0% | |
| GAAGGAGAGGAAGAGAGAAT+AGG | - | chr8.1:59897198-59897217 | MS.gene77769:intron | 45.0% | |
| GAGGATATTGACCCTCCAAA+TGG | - | chr8.1:59897687-59897706 | MS.gene77769:CDS | 45.0% | |
| GTGAGCTTAACTCAGTTGGT+AGG | + | chr8.1:59896775-59896794 | None:intergenic | 45.0% | |
| GTTCCATGTAGCCATTTGGA+GGG | + | chr8.1:59897701-59897720 | None:intergenic | 45.0% | |
| TGGATGTCCGTGTAGCAAAT+AGG | + | chr8.1:59897335-59897354 | None:intergenic | 45.0% | |
| TGTTCCATGTAGCCATTTGG+AGG | + | chr8.1:59897702-59897721 | None:intergenic | 45.0% | |
| ! | GGTGAGCTCAGTACTGAAAT+TGG | - | chr8.1:59896190-59896209 | MS.gene77769:CDS | 45.0% |
| !! | ATAAAAAATTATGAATTATT+TGG | - | chr8.1:59896984-59897003 | MS.gene77769:intron | 5.0% |
| !! | ATAATATTCATAAAAATAAT+AGG | - | chr8.1:59897358-59897377 | MS.gene77769:intron | 5.0% |
| !! | TAAAAAATTATGAATTATTT+GGG | - | chr8.1:59896985-59897004 | MS.gene77769:intron | 5.0% |
| !!! | ATTGAATAATAAATATTTTA+AGG | + | chr8.1:59897081-59897100 | None:intergenic | 5.0% |
| !!! | ATTTTTATGAATATTATAAA+TGG | + | chr8.1:59897355-59897374 | None:intergenic | 5.0% |
| ATGGCACTGCTGCTGCTAAA+TGG | - | chr8.1:59897640-59897659 | MS.gene77769:CDS | 50.0% | |
| GGTAGGGACAATGTAAACCC+CGG | + | chr8.1:59896758-59896777 | None:intergenic | 50.0% | |
| TGACCCTCCAAATGGCTACA+TGG | - | chr8.1:59897695-59897714 | MS.gene77769:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 59896178 | 59897782 | 59896178 | ID=MS.gene77769 |
| chr8.1 | mRNA | 59896178 | 59897782 | 59896178 | ID=MS.gene77769.t1;Parent=MS.gene77769 |
| chr8.1 | exon | 59897596 | 59897782 | 59897596 | ID=MS.gene77769.t1.exon1;Parent=MS.gene77769.t1 |
| chr8.1 | CDS | 59897596 | 59897782 | 59897596 | ID=cds.MS.gene77769.t1;Parent=MS.gene77769.t1 |
| chr8.1 | exon | 59896178 | 59896461 | 59896178 | ID=MS.gene77769.t1.exon2;Parent=MS.gene77769.t1 |
| chr8.1 | CDS | 59896178 | 59896461 | 59896178 | ID=cds.MS.gene77769.t1;Parent=MS.gene77769.t1 |
| Gene Sequence |
| Protein sequence |