AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77769


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77769.t1 MTR_8g045490 86.538 156 21 0 1 156 1 156 7.58e-101 286
MS.gene77769.t1 MTR_8g045520 77.707 157 34 1 1 156 1 157 6.23e-89 256
MS.gene77769.t1 MTR_8g045400 75.497 151 36 1 1 151 1 150 1.63e-84 245
MS.gene77769.t1 MTR_8g045570 74.834 151 38 0 1 151 1 151 2.88e-84 244
MS.gene77769.t1 MTR_8g045695 71.975 157 42 2 1 156 1 156 8.19e-82 238
MS.gene77769.t1 MTR_8g045300 71.711 152 43 0 1 152 1 152 1.73e-80 235
MS.gene77769.t1 MTR_8g045555 73.510 151 40 0 1 151 1 151 4.93e-80 233
MS.gene77769.t1 MTR_8g045735 70.199 151 44 1 1 151 1 150 1.01e-77 228
MS.gene77769.t1 MTR_8g045665 69.536 151 45 1 1 151 1 150 1.47e-75 222
MS.gene77769.t1 MTR_8g045640 68.153 157 48 2 1 156 1 156 1.75e-75 222
MS.gene77769.t1 MTR_8g045560 67.550 151 48 1 1 151 1 150 4.34e-73 216
MS.gene77769.t1 MTR_1g030810 44.156 154 83 2 1 152 1 153 2.27e-39 130
MS.gene77769.t1 MTR_1g030820 39.610 154 90 2 1 152 1 153 8.54e-38 126
MS.gene77769.t1 MTR_1g030840 36.478 159 93 4 1 152 1 158 4.81e-26 97.1
MS.gene77769.t1 MTR_8g012550 32.432 148 97 3 6 152 8 153 2.21e-25 95.1
MS.gene77769.t1 MTR_1g031640 36.054 147 85 4 7 151 7 146 7.28e-23 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77769.t1 AT5G28010 33.333 153 99 3 3 153 15 166 8.08e-24 91.7
MS.gene77769.t1 AT5G28010 33.333 153 99 3 3 153 15 166 8.08e-24 91.7
MS.gene77769.t1 AT1G70890 33.333 153 99 3 3 153 7 158 1.23e-21 85.9
MS.gene77769.t1 AT1G70840 30.921 152 102 3 3 152 20 170 5.17e-21 84.3
MS.gene77769.t1 AT5G28000 30.921 152 102 3 3 152 13 163 1.46e-20 83.2
MS.gene77769.t1 AT1G70830 31.373 153 102 3 3 153 22 173 3.03e-20 82.4
MS.gene77769.t1 AT1G23120 29.605 152 102 3 1 152 1 147 3.75e-20 81.6
MS.gene77769.t1 AT1G70830 31.373 153 102 3 3 153 22 173 5.70e-20 82.4
MS.gene77769.t1 AT1G23120 29.605 152 102 3 1 152 51 197 1.60e-19 81.3
MS.gene77769.t1 AT1G70870 44.792 96 52 1 56 151 44 138 1.91e-19 79.3
MS.gene77769.t1 AT1G70830 31.373 153 102 3 3 153 22 173 2.03e-19 82.0
MS.gene77769.t1 AT1G23130 29.801 151 104 2 3 152 10 159 2.60e-19 79.7
MS.gene77769.t1 AT1G70830 31.373 153 102 3 3 153 22 173 3.75e-19 82.4
MS.gene77769.t1 AT1G70830 30.065 153 104 3 3 153 184 335 1.32e-17 78.2
MS.gene77769.t1 AT4G23670 33.553 152 98 3 1 151 1 150 5.15e-18 76.3
MS.gene77769.t1 AT1G70830 30.065 153 104 3 3 153 137 288 6.45e-18 78.6
MS.gene77769.t1 AT1G70850 29.530 149 102 3 3 149 9 156 2.12e-17 76.3
MS.gene77769.t1 AT1G35260 30.137 146 98 3 6 149 5 148 4.18e-17 73.9
MS.gene77769.t1 AT1G70850 29.530 149 102 3 3 149 9 156 4.73e-17 76.6
MS.gene77769.t1 AT1G70850 30.769 143 96 3 13 153 175 316 9.70e-17 75.9
MS.gene77769.t1 AT1G70850 29.530 149 102 3 3 149 9 156 4.73e-17 76.6
MS.gene77769.t1 AT1G70850 30.769 143 96 3 13 153 175 316 9.70e-17 75.9
MS.gene77769.t1 AT1G70880 28.105 153 107 3 3 153 8 159 9.95e-17 73.2
MS.gene77769.t1 AT4G23680 30.263 152 103 3 1 151 1 150 1.28e-15 70.1
MS.gene77769.t1 AT2G01520 29.605 152 104 3 1 151 1 150 3.56e-15 68.9
MS.gene77769.t1 AT1G14930 30.818 159 93 5 1 151 1 150 1.38e-14 67.4
MS.gene77769.t1 AT1G35310 27.660 141 99 3 13 152 12 150 1.96e-14 66.6
MS.gene77769.t1 AT1G24020 30.769 156 106 2 1 155 1 155 2.42e-14 66.6
MS.gene77769.t1 AT1G24020 30.769 156 106 2 1 155 1 155 2.42e-14 66.6
MS.gene77769.t1 AT4G14060 30.263 152 103 3 1 151 1 150 2.93e-14 66.2
MS.gene77769.t1 AT2G01530 29.605 152 104 3 1 151 1 150 1.20e-13 64.7
MS.gene77769.t1 AT1G30990 30.370 135 91 3 1 134 1 133 4.86e-12 60.5
MS.gene77769.t1 AT1G14940 28.667 150 104 3 1 149 1 148 1.08e-11 59.7
MS.gene77769.t1 AT1G14950 26.623 154 110 3 1 153 1 152 3.14e-11 58.5

Find 23 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGCTACATGGAACATTT+TGG 0.264766 8.1:-59896233 MS.gene77769:CDS
ATCTTCACCTTCATGAAGTT+TGG 0.293563 8.1:+59897639 None:intergenic
ATGGCACTGCTGCTGCTAAA+TGG 0.314913 8.1:-59896298 MS.gene77769:CDS
GGTGAGCTCAGTACTGAAAT+TGG 0.365883 8.1:-59897748 MS.gene77769:CDS
AAATGTTCCATGTAGCCATT+TGG 0.413070 8.1:+59896236 None:intergenic
AACTTCATGAAGGTGAAGAT+TGG 0.483425 8.1:-59897636 MS.gene77769:CDS
AAACACTGGACTTATGTCAT+AGG 0.490184 8.1:-59897595 MS.gene77769:intron
AGATATTGATGAGCACTATA+AGG 0.500590 8.1:-59896357 MS.gene77769:CDS
TGACCCTCCAAATGGCTACA+TGG 0.521176 8.1:-59896243 MS.gene77769:CDS
GAGGATATTGACCCTCCAAA+TGG 0.540200 8.1:-59896251 MS.gene77769:CDS
TGTTCCATGTAGCCATTTGG+AGG 0.542543 8.1:+59896239 None:intergenic
AACACTTATAAGTTCTTCGG+TGG 0.555536 8.1:-59896380 MS.gene77769:CDS
TATGATGAACTCTTTCACAA+AGG 0.564152 8.1:+59897662 None:intergenic
GAAGTAATTGATAAGAGTGA+TGG 0.579637 8.1:-59896317 MS.gene77769:CDS
ATAATTCATAACAGATGGCA+AGG 0.593174 8.1:-59896453 MS.gene77769:intron
CATCATACCAAACTTCATGA+AGG 0.595182 8.1:-59897646 MS.gene77769:CDS
TATTGATGCTTATCTAGCCA+AGG 0.607429 8.1:-59896195 MS.gene77769:CDS
GTTCCATGTAGCCATTTGGA+GGG 0.622662 8.1:+59896240 None:intergenic
ACAGTGATTCAGTTAAACAC+TGG 0.626084 8.1:-59897609 MS.gene77769:CDS
CAGATTGTAGAACTTGTCAG+AGG 0.636689 8.1:+59897714 None:intergenic
TGAATATGAAAAGATCAATG+AGG 0.646793 8.1:-59896270 MS.gene77769:CDS
AGATTGTAGAACTTGTCAGA+GGG 0.675294 8.1:+59897715 None:intergenic
TTCTAAGAAATGGTGCTCTG+TGG 0.703436 8.1:-59897769 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGTCAAAAAAAAAAA+GGG + chr8.1:59897039-59897058 None:intergenic 10.0%
!! TATATTTGTCAAAAAAAAAA+AGG + chr8.1:59897040-59897059 None:intergenic 10.0%
!!! CTTTTAGATTTTTTTATTTT+TGG + chr8.1:59896675-59896694 None:intergenic 10.0%
!!! TTATTATTCAATTTTTTGTT+TGG - chr8.1:59897089-59897108 MS.gene77769:intron 10.0%
!! AGTAAAATAATAAGATGATT+TGG + chr8.1:59896401-59896420 None:intergenic 15.0%
!!! AAATATTTTAAGGTAAACAA+CGG + chr8.1:59897071-59897090 None:intergenic 15.0%
!!! AAATGTTGATCATTTTTAAT+TGG - chr8.1:59896466-59896485 MS.gene77769:intron 15.0%
!!! AATCATCTTATTATTTTACT+TGG - chr8.1:59896401-59896420 MS.gene77769:CDS 15.0%
!!! GATTCATATTTTTTTTTGAA+TGG + chr8.1:59896953-59896972 None:intergenic 15.0%
!!! TAGCAAGTTTTATTTTTTTT+TGG - chr8.1:59896729-59896748 MS.gene77769:intron 15.0%
!! AAAAACACTTATAAGTTCTT+CGG - chr8.1:59897555-59897574 MS.gene77769:intron 20.0%
!! AGGTTATTTGTAGAAAAAAA+AGG - chr8.1:59897378-59897397 MS.gene77769:intron 20.0%
!!! AAATAATAAGATGATTTGGT+TGG + chr8.1:59896397-59896416 None:intergenic 20.0%
!!! TTTTATTTGAATGTTTGCTT+TGG - chr8.1:59896549-59896568 MS.gene77769:intron 20.0%
!!! TTTTATTTTTTTTTGGTGAC+CGG - chr8.1:59896736-59896755 MS.gene77769:intron 20.0%
! TGAATATGAAAAGATCAATG+AGG - chr8.1:59897668-59897687 MS.gene77769:CDS 25.0%
!! TTGGTCTATATAAAGAGTTT+TGG + chr8.1:59896656-59896675 None:intergenic 25.0%
!!! TTTATTTTTTTTTGGTGACC+GGG - chr8.1:59896737-59896756 MS.gene77769:intron 25.0%
AACCATACTACTAGACTATT+AGG + chr8.1:59897306-59897325 None:intergenic 30.0%
AGATATTGATGAGCACTATA+AGG - chr8.1:59897581-59897600 MS.gene77769:intron 30.0%
ATAATTCATAACAGATGGCA+AGG - chr8.1:59897485-59897504 MS.gene77769:intron 30.0%
CCATCATAATTCATAACAGA+TGG - chr8.1:59897480-59897499 MS.gene77769:intron 30.0%
CCATCTGTTATGAATTATGA+TGG + chr8.1:59897483-59897502 None:intergenic 30.0%
GAAGTAATTGATAAGAGTGA+TGG - chr8.1:59897621-59897640 MS.gene77769:CDS 30.0%
TAGAGACAGACAATAACTTA+TGG - chr8.1:59897274-59897293 MS.gene77769:intron 30.0%
TATGATGAACTCTTTCACAA+AGG + chr8.1:59896279-59896298 None:intergenic 30.0%
TGTTATGAATTATGATGGCA+CGG + chr8.1:59897478-59897497 None:intergenic 30.0%
! TCATTAACCTATTTGCTACA+CGG - chr8.1:59897325-59897344 MS.gene77769:intron 30.0%
! TTATAGAGCTATCTTTTCCT+TGG + chr8.1:59897763-59897782 None:intergenic 30.0%
!!! TTATTTTTTTTTGGTGACCG+GGG - chr8.1:59896738-59896757 MS.gene77769:intron 30.0%
AAACACTGGACTTATGTCAT+AGG - chr8.1:59896343-59896362 MS.gene77769:CDS 35.0%
AAATGTTCCATGTAGCCATT+TGG + chr8.1:59897705-59897724 None:intergenic 35.0%
AACACTTATAAGTTCTTCGG+TGG - chr8.1:59897558-59897577 MS.gene77769:intron 35.0%
AACTTCATGAAGGTGAAGAT+TGG - chr8.1:59896302-59896321 MS.gene77769:CDS 35.0%
AATAGAGAGAAGAAGAGAGA+AGG - chr8.1:59897180-59897199 MS.gene77769:intron 35.0%
ACAGTGATTCAGTTAAACAC+TGG - chr8.1:59896329-59896348 MS.gene77769:CDS 35.0%
AGATTGTAGAACTTGTCAGA+GGG + chr8.1:59896226-59896245 None:intergenic 35.0%
AGCCTAATAGTCTAGTAGTA+TGG - chr8.1:59897301-59897320 MS.gene77769:intron 35.0%
ATCTTCACCTTCATGAAGTT+TGG + chr8.1:59896302-59896321 None:intergenic 35.0%
CATCATACCAAACTTCATGA+AGG - chr8.1:59896292-59896311 MS.gene77769:CDS 35.0%
GTTATGAATTATGATGGCAC+GGG + chr8.1:59897477-59897496 None:intergenic 35.0%
TATTGATGCTTATCTAGCCA+AGG - chr8.1:59897743-59897762 MS.gene77769:CDS 35.0%
! AATGGCTACATGGAACATTT+TGG - chr8.1:59897705-59897724 MS.gene77769:CDS 35.0%
! TAACATTTTGCCTATGTAGG+TGG + chr8.1:59896633-59896652 None:intergenic 35.0%
! TGGTAACATTTTGCCTATGT+AGG + chr8.1:59896636-59896655 None:intergenic 35.0%
AAGGAGAGGAAGAGAGAATA+GGG - chr8.1:59897199-59897218 MS.gene77769:intron 40.0%
ACACAAAAGACCACCTACAT+AGG - chr8.1:59896620-59896639 MS.gene77769:intron 40.0%
CAGATTGTAGAACTTGTCAG+AGG + chr8.1:59896227-59896246 None:intergenic 40.0%
! TGAGCTTAACTCAGTTGGTA+GGG + chr8.1:59896774-59896793 None:intergenic 40.0%
AGAGAAGAAGAGAGAAGGAG+AGG - chr8.1:59897185-59897204 MS.gene77769:intron 45.0%
CCAACTGAGTTAAGCTCACA+AGG - chr8.1:59896776-59896795 MS.gene77769:intron 45.0%
CCTTGTGAGCTTAACTCAGT+TGG + chr8.1:59896779-59896798 None:intergenic 45.0%
GAAGGAGAGGAAGAGAGAAT+AGG - chr8.1:59897198-59897217 MS.gene77769:intron 45.0%
GAGGATATTGACCCTCCAAA+TGG - chr8.1:59897687-59897706 MS.gene77769:CDS 45.0%
GTGAGCTTAACTCAGTTGGT+AGG + chr8.1:59896775-59896794 None:intergenic 45.0%
GTTCCATGTAGCCATTTGGA+GGG + chr8.1:59897701-59897720 None:intergenic 45.0%
TGGATGTCCGTGTAGCAAAT+AGG + chr8.1:59897335-59897354 None:intergenic 45.0%
TGTTCCATGTAGCCATTTGG+AGG + chr8.1:59897702-59897721 None:intergenic 45.0%
! GGTGAGCTCAGTACTGAAAT+TGG - chr8.1:59896190-59896209 MS.gene77769:CDS 45.0%
!! ATAAAAAATTATGAATTATT+TGG - chr8.1:59896984-59897003 MS.gene77769:intron 5.0%
!! ATAATATTCATAAAAATAAT+AGG - chr8.1:59897358-59897377 MS.gene77769:intron 5.0%
!! TAAAAAATTATGAATTATTT+GGG - chr8.1:59896985-59897004 MS.gene77769:intron 5.0%
!!! ATTGAATAATAAATATTTTA+AGG + chr8.1:59897081-59897100 None:intergenic 5.0%
!!! ATTTTTATGAATATTATAAA+TGG + chr8.1:59897355-59897374 None:intergenic 5.0%
ATGGCACTGCTGCTGCTAAA+TGG - chr8.1:59897640-59897659 MS.gene77769:CDS 50.0%
GGTAGGGACAATGTAAACCC+CGG + chr8.1:59896758-59896777 None:intergenic 50.0%
TGACCCTCCAAATGGCTACA+TGG - chr8.1:59897695-59897714 MS.gene77769:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 59896178 59897782 59896178 ID=MS.gene77769
chr8.1 mRNA 59896178 59897782 59896178 ID=MS.gene77769.t1;Parent=MS.gene77769
chr8.1 exon 59897596 59897782 59897596 ID=MS.gene77769.t1.exon1;Parent=MS.gene77769.t1
chr8.1 CDS 59897596 59897782 59897596 ID=cds.MS.gene77769.t1;Parent=MS.gene77769.t1
chr8.1 exon 59896178 59896461 59896178 ID=MS.gene77769.t1.exon2;Parent=MS.gene77769.t1
chr8.1 CDS 59896178 59896461 59896178 ID=cds.MS.gene77769.t1;Parent=MS.gene77769.t1
Gene Sequence

>MS.gene77769

ATGGTGCTCTGTGGTGAGCTCAGTACTGAAATTGGAATCAAAACACCCTCTGACAAGTTCTACAATCTGTTCATAACAGAACTTCATGAAGTGCAAAACCTTTGTGAAAGAGTTCATCATACCAAACTTCATGAAGGTGAAGATTGGCATCACAGTGATTCAGTTAAACACTGGACTTATGTCATAGGTAATTATGTTTCTAATTCAATTAACTTGCCAACCAAATCATCTTATTATTTTACTTGGATTTTTCTATCATTAAGTTTGTTTGAAGTTTCGTTAAAACAAAAATGTTGATCATTTTTAATTGGTTTATGTGTGATTTTCGTCTAATACAAATATATTTTCTCTATTTAAATGTTCTATCATTTTTTTATTTGAATGTTTGCTTTGGTAGTTCAAAAAATAATTCAAGTAATAAATAATCAATATAATTAACTACACACAAAAGACCACCTACATAGGCAAAATGTTACCAAAACTCTTTATATAGACCAAAAATAAAAAAATCTAAAAGCAAAAGCAAACTAGATAAATACTGAGATATTAATTAGCAAGTTTTATTTTTTTTTGGTGACCGGGGTTTACATTGTCCCTACCAACTGAGTTAAGCTCACAAGGACAATTTTAGCAAGTTTTAAGTACAATGTTAAAAATAATTTGATAGATTTAATTTGTAAATATTGTTAATATAGATTATGAGATCTCAAAATATATTCTATAAATAAAAAGTAATGAAAAGAACTATATAACAATTATGAATTATTTACTGCCATTCAAAAAAAAATATGAATCATTTACTGTAAATAAAAAATTATGAATTATTTGGGAGATTAAAATTAATCATATAAAGAATAACCCTTTTTTTTTTTGACAAATATAAAGAATAACCGTTGTTTACCTTAAAATATTTATTATTCAATTTTTTGTTTGGAAAATTATTTATTAGAAAAAAGAAAACAATATAATTGTTATAAAACTCATAGAATAATAATTAATAAGAATAGAGAGAAGAAGAGAGAAGGAGAGGAAGAGAGAATAGGGCATTCTATTATTCTCATATGTGCACAAGTATGTTACATGTAAACTTTATTTATAGAGACAGACAATAACTTATGGAACAAGCCTAATAGTCTAGTAGTATGGTTCATTAACCTATTTGCTACACGGACATCCATTTATAATATTCATAAAAATAATAGGTTATTTGTAGAAAAAAAAGGCTTGCATTTTTCTATAGTTATTTTTACAAGTGCATTTTTCTATAGTTGTTTTCATTTTTATCTGTATTAATTGCCCGTGCCATCATAATTCATAACAGATGGCAAGGTACACACATGTCATGAGAGTGTTGAAGAAATTGATGAACTGAACAAAAAAAACACTTATAAGTTCTTCGGTGGAGATATTGATGAGCACTATAAGGTCTTTAAGCTCATTCTTGAAGTAATTGATAAGAGTGATGGCACTGCTGCTGCTAAATGGACTATTGAATATGAAAAGATCAATGAGGATATTGACCCTCCAAATGGCTACATGGAACATTTTGGCAAATGCACTCGAGATATTGATGCTTATCTAGCCAAGGAAAAGATAGCTCTATAA

Protein sequence

>MS.gene77769.t1

MVLCGELSTEIGIKTPSDKFYNLFITELHEVQNLCERVHHTKLHEGEDWHHSDSVKHWTYVIDGKVHTCHESVEEIDELNKKNTYKFFGGDIDEHYKVFKLILEVIDKSDGTAAAKWTIEYEKINEDIDPPNGYMEHFGKCTRDIDAYLAKEKIAL