AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74358


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74358.t1 MTR_8g045490 83.030 165 19 1 1 165 1 156 9.94e-96 274
MS.gene74358.t1 MTR_8g045520 72.892 166 35 2 1 165 1 157 1.67e-83 243
MS.gene74358.t1 MTR_8g045400 69.880 166 39 3 1 165 1 156 2.74e-78 229
MS.gene74358.t1 MTR_8g045570 68.323 161 42 1 1 161 1 152 5.06e-78 229
MS.gene74358.t1 MTR_8g045555 66.460 161 45 1 1 161 1 152 6.05e-74 218
MS.gene74358.t1 MTR_8g045695 66.049 162 45 2 1 162 1 152 6.40e-74 218
MS.gene74358.t1 MTR_8g045300 63.030 165 51 2 1 164 1 156 1.39e-72 215
MS.gene74358.t1 MTR_8g045735 62.963 162 50 2 1 162 1 152 2.14e-70 209
MS.gene74358.t1 MTR_8g045665 62.346 162 51 2 1 162 1 152 4.19e-68 204
MS.gene74358.t1 MTR_8g045640 61.728 162 52 2 1 162 1 152 3.47e-67 201
MS.gene74358.t1 MTR_8g045560 60.494 162 54 2 1 162 1 152 3.26e-65 196
MS.gene74358.t1 MTR_1g030820 37.037 162 90 3 1 160 1 152 2.54e-32 113
MS.gene74358.t1 MTR_1g030810 37.654 162 89 3 1 160 1 152 5.66e-32 112
MS.gene74358.t1 MTR_8g012550 32.258 155 93 4 6 159 8 151 4.43e-22 87.0
MS.gene74358.t1 MTR_1g030840 32.121 165 95 5 1 158 1 155 6.20e-21 84.3
MS.gene74358.t1 MTR_1g031640 33.758 157 86 6 7 161 7 147 1.97e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74358.t1 AT5G28010 33.544 158 93 4 3 158 15 162 3.28e-21 85.1
MS.gene74358.t1 AT5G28010 33.544 158 93 4 3 158 15 162 3.28e-21 85.1
MS.gene74358.t1 AT1G70890 34.177 158 92 5 3 158 7 154 9.58e-21 83.6
MS.gene74358.t1 AT1G70870 42.268 97 55 1 64 160 43 138 6.43e-19 78.6
MS.gene74358.t1 AT1G70830 31.646 158 96 4 3 158 22 169 3.24e-18 77.4
MS.gene74358.t1 AT1G70830 31.646 158 96 4 3 158 22 169 6.36e-18 77.4
MS.gene74358.t1 AT1G70840 29.747 158 99 4 3 158 20 167 1.10e-17 76.3
MS.gene74358.t1 AT1G70830 31.646 158 96 4 3 158 22 169 2.03e-17 77.0
MS.gene74358.t1 AT1G70830 31.646 158 96 4 3 158 22 169 3.47e-17 77.4
MS.gene74358.t1 AT5G28000 29.747 158 99 4 3 158 13 160 2.61e-16 72.4
MS.gene74358.t1 AT1G23120 29.605 152 93 4 7 158 7 144 8.21e-16 70.5
MS.gene74358.t1 AT1G70850 29.747 158 99 4 3 158 9 156 8.68e-16 72.4
MS.gene74358.t1 AT1G70850 29.747 158 99 4 3 158 9 156 1.49e-15 72.8
MS.gene74358.t1 AT1G70850 29.730 148 92 4 13 158 175 312 2.23e-14 69.3
MS.gene74358.t1 AT1G70850 29.747 158 99 4 3 158 9 156 1.49e-15 72.8
MS.gene74358.t1 AT1G70850 29.730 148 92 4 13 158 175 312 2.23e-14 69.3
MS.gene74358.t1 AT1G23120 38.462 91 55 1 68 158 105 194 1.99e-15 70.9
MS.gene74358.t1 AT4G23670 30.864 162 100 3 1 161 1 151 2.53e-15 69.3
MS.gene74358.t1 AT1G24020 32.927 164 101 4 1 164 1 155 1.32e-14 67.8
MS.gene74358.t1 AT1G24020 32.927 164 101 4 1 164 1 155 1.32e-14 67.8
MS.gene74358.t1 AT1G23130 26.752 157 104 3 3 158 10 156 2.22e-14 67.0
MS.gene74358.t1 AT1G70880 27.215 158 103 3 3 158 8 155 2.58e-14 67.0
MS.gene74358.t1 AT4G23680 29.012 162 103 3 1 161 1 151 2.27e-13 64.3
MS.gene74358.t1 AT2G01520 29.630 162 102 4 1 161 1 151 2.83e-13 63.9
MS.gene74358.t1 AT4G14060 31.481 162 99 4 1 161 1 151 1.30e-12 62.4
MS.gene74358.t1 AT1G35260 27.273 154 99 5 7 158 6 148 2.06e-12 61.6
MS.gene74358.t1 AT1G14930 30.588 170 92 6 1 162 1 152 3.02e-12 61.2
MS.gene74358.t1 AT2G01530 29.630 162 102 4 1 161 1 151 6.66e-12 60.5

Find 26 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAGGTTCTCTGGGGAATA+TGG 0.306691 8.4:-55807888 None:intergenic
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8.4:+55808469 MS.gene74358:CDS
CACCCTTATCAATGACTTCA+AGG 0.380806 8.4:-55808446 None:intergenic
TTATAGAGCTATCTTTGCTT+TGG 0.392094 8.4:-55808589 None:intergenic
TTCTAAGAAATGGTGCTCTC+TGG 0.436928 8.4:+55807355 None:intergenic
CATCCTTGAAGTCATTGATA+AGG 0.445764 8.4:+55808443 MS.gene74358:CDS
AAGTATTCCATGTAGCCATT+TGG 0.448595 8.4:-55808531 None:intergenic
AACACTTATAAGCTCTTTGG+TGG 0.457999 8.4:+55808387 MS.gene74358:CDS
AACTTCATGAAGGTGAAGAT+TGG 0.478377 8.4:+55807488 MS.gene74358:CDS
GGTAAGCTCAGTACTGAAGT+TGG 0.490802 8.4:+55807376 MS.gene74358:CDS
ATCCTTGAAGTCATTGATAA+GGG 0.494628 8.4:+55808444 MS.gene74358:CDS
AGATATTGATGAGCACTATA+AGG 0.500590 8.4:+55808410 MS.gene74358:CDS
GAACAGATTGTAGAACTTGT+CGG 0.501167 8.4:-55807413 None:intergenic
TATTCCATGTAGCCATTTGG+AGG 0.517921 8.4:-55808528 None:intergenic
AGATTGGCATCACACTGATT+CGG 0.519723 8.4:+55807504 MS.gene74358:CDS
AATGGCTACATGGAATACTT+TGG 0.525683 8.4:+55808534 MS.gene74358:CDS
TGACCCTCCAAATGGCTACA+TGG 0.526835 8.4:+55808524 MS.gene74358:CDS
GAGGATATTGACCCTCCAAA+TGG 0.540200 8.4:+55808516 MS.gene74358:CDS
TATGATGAACTCTTTCACAA+AGG 0.552779 8.4:-55807462 None:intergenic
GAAGTCATTGATAAGGGTGA+TGG 0.579318 8.4:+55808450 MS.gene74358:CDS
ATCATAATTCACAGATGGCA+AGG 0.582682 8.4:+55808314 MS.gene74358:intron
CATCATACAAAACTTCATGA+AGG 0.606616 8.4:+55807478 MS.gene74358:CDS
ATTCCATGTAGCCATTTGGA+GGG 0.625101 8.4:-55808527 None:intergenic
AGATTGTAGAACTTGTCGGC+GGG 0.646397 8.4:-55807409 None:intergenic
TGAATATGAAAAGATCAATG+AGG 0.667026 8.4:+55808497 MS.gene74358:CDS
CAGATTGTAGAACTTGTCGG+CGG 0.688816 8.4:-55807410 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGAATAATAAATATTTATA+AGG - chr8.4:55808158-55808177 None:intergenic 10.0%
!!! ATAGTTTATTTGTTAAAAAA+TGG + chr8.4:55808223-55808242 MS.gene74358:intron 10.0%
!!! TAGTTTATTTGTTAAAAAAT+GGG + chr8.4:55808224-55808243 MS.gene74358:intron 10.0%
!! AAAAAAAATCAACAATCATT+TGG + chr8.4:55807914-55807933 MS.gene74358:CDS 15.0%
!! AAAAAAATCAACAATCATTT+GGG + chr8.4:55807915-55807934 MS.gene74358:CDS 15.0%
!! AATTAAATAAGATGATGATT+TGG - chr8.4:55807591-55807610 None:intergenic 15.0%
!! ACAAATAAAAATGAAAACTT+TGG - chr8.4:55808278-55808297 None:intergenic 15.0%
!!! TGTTGATTTTTTTTGTCATA+AGG - chr8.4:55807908-55807927 None:intergenic 20.0%
! AAAAACACTTATAAGCTCTT+TGG + chr8.4:55808384-55808403 MS.gene74358:CDS 25.0%
! AGATTATGAGATCTCAAAAA+TGG + chr8.4:55808011-55808030 MS.gene74358:intron 25.0%
! ATAAGAAAGAAACAAGTACA+TGG + chr8.4:55807695-55807714 MS.gene74358:intron 25.0%
! TGAATATGAAAAGATCAATG+AGG + chr8.4:55808497-55808516 MS.gene74358:CDS 25.0%
! TTGGGAAGAAAAAAAAATCA+GGG - chr8.4:55807778-55807797 None:intergenic 25.0%
! TTTGGGAAGAAAAAAAAATC+AGG - chr8.4:55807779-55807798 None:intergenic 25.0%
!! AACCACAGTTTTTCTTTATA+TGG - chr8.4:55808133-55808152 None:intergenic 25.0%
!! CTATCTTGAAAATGGTTTTT+GGG - chr8.4:55807796-55807815 None:intergenic 25.0%
!! TCTATCTTGAAAATGGTTTT+TGG - chr8.4:55807797-55807816 None:intergenic 25.0%
AACATAGTCAAAAGTAGAAG+AGG - chr8.4:55807839-55807858 None:intergenic 30.0%
AGATATTGATGAGCACTATA+AGG + chr8.4:55808410-55808429 MS.gene74358:CDS 30.0%
AGCCATATAAAGAAAAACTG+TGG + chr8.4:55808128-55808147 MS.gene74358:intron 30.0%
ATCCTTGAAGTCATTGATAA+GGG + chr8.4:55808444-55808463 MS.gene74358:CDS 30.0%
CATCATACAAAACTTCATGA+AGG + chr8.4:55807478-55807497 MS.gene74358:CDS 30.0%
TACATTCTACAAACCAAACA+TGG + chr8.4:55807748-55807767 MS.gene74358:intron 30.0%
TATGATGAACTCTTTCACAA+AGG - chr8.4:55807465-55807484 None:intergenic 30.0%
TGGGAAGAAAAAAAAATCAG+GGG - chr8.4:55807777-55807796 None:intergenic 30.0%
TTATAGAGCTATCTTTGCTT+TGG - chr8.4:55808592-55808611 None:intergenic 30.0%
! TGAAGCATCTATCTTGAAAA+TGG - chr8.4:55807804-55807823 None:intergenic 30.0%
! TTTTTGTCATAAGGTTCTCT+GGG - chr8.4:55807899-55807918 None:intergenic 30.0%
!! CTACTTTTGACTATGTTGAA+CGG + chr8.4:55807842-55807861 MS.gene74358:intron 30.0%
!! TACTTTTGACTATGTTGAAC+GGG + chr8.4:55807843-55807862 MS.gene74358:intron 30.0%
!! TGATGATTTGGTTATTGATC+AGG - chr8.4:55807579-55807598 None:intergenic 30.0%
!!! TTTTTTGTCATAAGGTTCTC+TGG - chr8.4:55807900-55807919 None:intergenic 30.0%
AACACTTATAAGCTCTTTGG+TGG + chr8.4:55808387-55808406 MS.gene74358:CDS 35.0%
AACTTCATGAAGGTGAAGAT+TGG + chr8.4:55807488-55807507 MS.gene74358:CDS 35.0%
AAGTATTCCATGTAGCCATT+TGG - chr8.4:55808534-55808553 None:intergenic 35.0%
AATGGCTACATGGAATACTT+TGG + chr8.4:55808534-55808553 MS.gene74358:CDS 35.0%
ATCATAATTCACAGATGGCA+AGG + chr8.4:55808314-55808333 MS.gene74358:intron 35.0%
ATCTGTGAATTATGATGGCA+CGG - chr8.4:55808310-55808329 None:intergenic 35.0%
CATCCTTGAAGTCATTGATA+AGG + chr8.4:55808443-55808462 MS.gene74358:CDS 35.0%
GAACAGATTGTAGAACTTGT+CGG - chr8.4:55807416-55807435 None:intergenic 35.0%
TTGCCATCTGTGAATTATGA+TGG - chr8.4:55808315-55808334 None:intergenic 35.0%
! TTTTGTCATAAGGTTCTCTG+GGG - chr8.4:55807898-55807917 None:intergenic 35.0%
AAATCAGGGGTAACCATGTT+TGG - chr8.4:55807764-55807783 None:intergenic 40.0%
ATTCCATGTAGCCATTTGGA+GGG - chr8.4:55808530-55808549 None:intergenic 40.0%
CACCCTTATCAATGACTTCA+AGG - chr8.4:55808449-55808468 None:intergenic 40.0%
GAAGTCATTGATAAGGGTGA+TGG + chr8.4:55808450-55808469 MS.gene74358:CDS 40.0%
GTGCCATCATAATTCACAGA+TGG + chr8.4:55808309-55808328 MS.gene74358:intron 40.0%
TATTCCATGTAGCCATTTGG+AGG - chr8.4:55808531-55808550 None:intergenic 40.0%
TCTGTGAATTATGATGGCAC+GGG - chr8.4:55808309-55808328 None:intergenic 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8.4:55807504-55807523 MS.gene74358:CDS 40.0%
! ATAAGGTTCTCTGGGGAATA+TGG - chr8.4:55807891-55807910 None:intergenic 40.0%
!! ACACTGATTCGGTTAAACAC+TGG + chr8.4:55807515-55807534 MS.gene74358:intron 40.0%
AAACACTGGACTTACGTCGT+AGG + chr8.4:55807529-55807548 MS.gene74358:intron 45.0%
AGATTGTAGAACTTGTCGGC+GGG - chr8.4:55807412-55807431 None:intergenic 45.0%
CAGATTGTAGAACTTGTCGG+CGG - chr8.4:55807413-55807432 None:intergenic 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8.4:55808516-55808535 MS.gene74358:CDS 45.0%
! GGTAAGCTCAGTACTGAAGT+TGG + chr8.4:55807376-55807395 MS.gene74358:CDS 45.0%
!!! TATTTTTGTATAAAAAAAAA+AGG - chr8.4:55808178-55808197 None:intergenic 5.0%
!!! TTTTATACAAAAATATTTAT+TGG + chr8.4:55808183-55808202 MS.gene74358:intron 5.0%
ATGGCACTGCTGCTGCTAAA+TGG + chr8.4:55808469-55808488 MS.gene74358:CDS 50.0%
TGACCCTCCAAATGGCTACA+TGG + chr8.4:55808524-55808543 MS.gene74358:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 55807364 55808611 55807364 ID=MS.gene74358
chr8.4 mRNA 55807364 55808611 55807364 ID=MS.gene74358.t1;Parent=MS.gene74358
chr8.4 exon 55807364 55807525 55807364 ID=MS.gene74358.t1.exon1;Parent=MS.gene74358.t1
chr8.4 CDS 55807364 55807525 55807364 ID=cds.MS.gene74358.t1;Parent=MS.gene74358.t1
chr8.4 exon 55807885 55807936 55807885 ID=MS.gene74358.t1.exon2;Parent=MS.gene74358.t1
chr8.4 CDS 55807885 55807936 55807885 ID=cds.MS.gene74358.t1;Parent=MS.gene74358.t1
chr8.4 exon 55808328 55808611 55808328 ID=MS.gene74358.t1.exon3;Parent=MS.gene74358.t1
chr8.4 CDS 55808328 55808611 55808328 ID=cds.MS.gene74358.t1;Parent=MS.gene74358.t1
Gene Sequence

>MS.gene74358

ATGGTGCTCTCTGGTAAGCTCAGTACTGAAGTTGGAATCAAAACACCCGCCGACAAGTTCTACAATCTGTTCATAACAGAACTTCATGAAGTGCAAAACCTTTGTGAAAGAGTTCATCATACAAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCGGTTAAACACTGGACTTACGTCGTAGGTAATTATGTTTCTAATTCAGTTCACCTGATCAATAACCAAATCATCATCTTATTTAATTTGAAATTTTTTTTATGTTTTATTTTTATTCTTAATGATAACTATACTTCTAATAACAAAAAAAAAATTGTGAATTAAAAGAAATATAAGAAAGAAACAAGTACATGGTTACTTATCAATACATGAATTACTTATCAATACATTCTACAAACCAAACATGGTTACCCCTGATTTTTTTTTCTTCCCAAAAACCATTTTCAAGATAGATGCTTCATCATTTCAATCACCTCTTCTACTTTTGACTATGTTGAACGGGAAATATAATCATTCATAAGCTTCCATATTCCCCAGAGAACCTTATGACAAAAAAAATCAACAATCATTTGGGTAACTCATCTCTTAAAGTTTTAAATACAATGTTAACAAATGTGTTCAATTTAATTTGTAAATTTTGTTAATATAGATTATGAGATCTCAAAAATGGTTAAATGAATAAAAAGTAATGAAAAGAAGTATATAAAAATCATGAATTATGTAAAAAAAAAAAACAATTATGAATTATTTGAGAGATTAAAATCAGCCATATAAAGAAAAACTGTGGTTTACCTTATAAATATTTATTATTCCTTTTTTTTTTATACAAAAATATTTATTGGTAAAATAAAACAATATAATAGTTTATTTGTTAAAAAATGGGTGATTGTATTTTTCTGTAGTTGCTATATCCAAAGTTTTCATTTTTATTTGTATTAATTTCCCGTGCCATCATAATTCACAGATGGCAAGGTACATACATGTCACGAGAGAGTTGAAGAAGTTGATGAACTGAATAAAAAAAACACTTATAAGCTCTTTGGTGGAGATATTGATGAGCACTATAAGGTCTTTAAGCTCATCCTTGAAGTCATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAAAAGATCAATGAGGATATTGACCCTCCAAATGGCTACATGGAATACTTTGGCAAAATCACTAGAGATGTTGATGCTCATCTTGCCAAAGCAAAGATAGCTCTATAA

Protein sequence

>MS.gene74358.t1

MVLSGKLSTEVGIKTPADKFYNLFITELHEVQNLCERVHHTKLHEGEDWHHTDSLPYSPENLMTKKINNHLDGKVHTCHERVEEVDELNKKNTYKLFGGDIDEHYKVFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGYMEYFGKITRDVDAHLAKAKIAL