AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74341


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74341.t1 MTR_8g045695 96.154 156 6 0 1 156 1 156 6.75e-111 311
MS.gene74341.t1 MTR_8g045735 88.312 154 18 0 1 154 1 154 1.42e-101 288
MS.gene74341.t1 MTR_8g045665 87.179 156 20 0 1 156 1 156 7.03e-100 284
MS.gene74341.t1 MTR_8g045640 83.333 156 26 0 1 156 1 156 6.99e-96 274
MS.gene74341.t1 MTR_8g045560 82.692 156 27 0 1 156 1 156 1.32e-94 270
MS.gene74341.t1 MTR_8g045520 78.981 157 32 1 1 156 1 157 1.48e-89 258
MS.gene74341.t1 MTR_8g045490 75.159 157 37 2 1 156 1 156 9.45e-86 248
MS.gene74341.t1 MTR_8g045400 73.248 157 40 2 1 156 1 156 3.48e-83 241
MS.gene74341.t1 MTR_8g045570 71.711 152 42 1 1 151 1 152 9.41e-81 235
MS.gene74341.t1 MTR_8g045555 71.053 152 43 1 1 151 1 152 7.08e-80 233
MS.gene74341.t1 MTR_8g045300 67.949 156 49 1 1 155 1 156 6.56e-79 231
MS.gene74341.t1 MTR_1g030810 43.137 153 83 3 1 150 1 152 1.54e-39 131
MS.gene74341.t1 MTR_1g030820 38.562 153 90 3 1 150 1 152 1.21e-37 126
MS.gene74341.t1 MTR_1g030840 38.462 156 87 5 1 148 1 155 6.28e-29 104
MS.gene74341.t1 MTR_8g012550 34.000 150 95 4 6 153 8 155 2.32e-26 97.8
MS.gene74341.t1 MTR_1g031640 38.514 148 81 6 7 151 7 147 1.88e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74341.t1 AT5G28010 35.948 153 94 4 3 152 15 166 1.27e-25 96.3
MS.gene74341.t1 AT5G28010 35.948 153 94 4 3 152 15 166 1.27e-25 96.3
MS.gene74341.t1 AT1G70840 35.294 153 95 4 3 152 20 171 9.76e-25 94.0
MS.gene74341.t1 AT1G70830 36.601 153 93 4 3 152 22 173 2.61e-24 92.8
MS.gene74341.t1 AT1G70830 36.364 154 94 4 3 153 22 174 3.83e-24 93.2
MS.gene74341.t1 AT1G70890 35.948 153 94 4 3 152 7 158 6.07e-24 91.7
MS.gene74341.t1 AT1G70830 36.364 154 94 4 3 153 22 174 1.70e-23 92.8
MS.gene74341.t1 AT1G70830 36.364 154 94 4 3 153 22 174 4.34e-23 93.2
MS.gene74341.t1 AT1G70830 35.294 153 95 4 3 152 184 335 1.52e-21 89.0
MS.gene74341.t1 AT5G28000 32.680 153 99 4 3 152 13 164 2.82e-22 87.4
MS.gene74341.t1 AT1G70830 35.294 153 95 4 3 152 137 288 5.28e-22 89.7
MS.gene74341.t1 AT1G70830 32.990 97 63 2 59 153 31 127 3.56e-11 60.1
MS.gene74341.t1 AT1G70850 34.667 150 94 4 3 149 9 157 1.04e-21 87.8
MS.gene74341.t1 AT1G70850 34.667 150 94 4 3 149 9 157 2.33e-21 88.2
MS.gene74341.t1 AT1G70850 35.664 143 88 4 13 152 175 316 7.32e-21 87.0
MS.gene74341.t1 AT1G70850 34.667 150 94 4 3 149 9 157 2.33e-21 88.2
MS.gene74341.t1 AT1G70850 35.664 143 88 4 13 152 175 316 7.32e-21 87.0
MS.gene74341.t1 AT4G23670 32.680 153 99 4 1 151 1 151 1.99e-20 82.4
MS.gene74341.t1 AT1G14930 29.299 157 107 4 1 155 1 155 2.63e-19 79.7
MS.gene74341.t1 AT1G70870 34.839 155 79 5 1 150 1 138 6.98e-19 78.2
MS.gene74341.t1 AT2G01520 29.412 153 104 4 1 151 1 151 1.07e-18 77.8
MS.gene74341.t1 AT2G01530 30.719 153 102 4 1 151 1 151 9.47e-18 75.5
MS.gene74341.t1 AT1G70880 29.412 153 104 4 3 152 8 159 3.86e-17 73.9
MS.gene74341.t1 AT1G14940 28.662 157 108 4 1 155 1 155 5.89e-17 73.6
MS.gene74341.t1 AT1G35260 30.612 147 91 5 9 149 8 149 2.25e-16 72.0
MS.gene74341.t1 AT4G14060 28.758 153 105 4 1 151 1 151 2.29e-16 72.0
MS.gene74341.t1 AT4G23680 28.947 152 104 4 1 150 1 150 2.93e-16 71.6
MS.gene74341.t1 AT1G23130 28.188 149 104 3 3 149 10 157 4.69e-16 71.2
MS.gene74341.t1 AT1G24020 30.968 155 98 4 1 150 1 151 4.89e-16 71.2
MS.gene74341.t1 AT1G24020 30.968 155 98 4 1 150 1 151 4.89e-16 71.2
MS.gene74341.t1 AT1G14950 28.025 157 109 4 1 155 1 155 7.89e-16 70.5
MS.gene74341.t1 AT1G23120 28.667 150 99 5 3 149 1 145 1.71e-15 69.3
MS.gene74341.t1 AT1G23120 27.660 141 96 4 10 149 60 195 4.49e-15 69.7
MS.gene74341.t1 AT1G30990 27.815 151 104 5 1 149 1 148 1.52e-13 64.3
MS.gene74341.t1 AT1G14960 26.452 155 110 4 1 153 1 153 8.47e-13 62.4
MS.gene74341.t1 AT1G35310 27.338 139 97 4 13 149 12 148 1.56e-12 62.0
MS.gene74341.t1 AT3G26460 26.797 153 109 3 1 151 1 152 2.21e-12 61.6
MS.gene74341.t1 AT3G26450 24.675 154 111 5 1 151 1 152 4.16e-11 58.2

Find 28 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACATACAAGCTATT+TGG 0.273919 8.4:+55475356 MS.gene74341:CDS
TGGAGATATTGAAAACTATA+AGG 0.391039 8.4:+55475379 MS.gene74341:CDS
ATGGAAATTCTGCTGTTAGA+TGG 0.411619 8.4:+55475438 MS.gene74341:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8.4:+55474146 MS.gene74341:CDS
AGGTAGTCCATCCAGCCATT+TGG 0.456227 8.4:-55475500 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 8.4:+55474299 MS.gene74341:CDS
AGCTTCATGAGGGTGAAGAT+TGG 0.493681 8.4:+55474258 MS.gene74341:CDS
TCATCTTGTCAAAGGGGAAA+AGG 0.504175 8.4:+55475550 MS.gene74341:CDS
AGATTGGCATCATACTGATT+CGG 0.504369 8.4:+55474274 MS.gene74341:CDS
AAGAGCACTAGAGATATTGA+TGG 0.505384 8.4:+55475527 MS.gene74341:CDS
TTGATGGTCATCTTGTCAAA+GGG 0.506740 8.4:+55475543 MS.gene74341:CDS
ATCTTCACCCTCATGAAGCT+TGG 0.519812 8.4:-55474255 None:intergenic
ATCACATACAAGCTATTTGG+TGG 0.530005 8.4:+55475359 MS.gene74341:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8.4:+55474147 MS.gene74341:CDS
ATTGATGGTCATCTTGTCAA+AGG 0.543582 8.4:+55475542 MS.gene74341:CDS
AGTTTGTAGAACTTGTCAGC+TGG 0.569465 8.4:-55474179 None:intergenic
TATCTCTAGTGCTCTTGCTA+AGG 0.569595 8.4:-55475520 None:intergenic
GAAGTGCTTAACAAGGCTGA+TGG 0.573383 8.4:+55475419 MS.gene74341:CDS
ATGTTGATACTCCAAATGGC+TGG 0.578150 8.4:+55475489 MS.gene74341:CDS
TGATACTCCAAATGGCTGGA+TGG 0.587093 8.4:+55475493 MS.gene74341:CDS
GAGGATGTTGATACTCCAAA+TGG 0.593330 8.4:+55475485 MS.gene74341:CDS
ATACTGATTCGGTTAAACAC+TGG 0.606046 8.4:+55474285 MS.gene74341:CDS
TCATCATACCAAGCTTCATG+AGG 0.610699 8.4:+55474247 MS.gene74341:CDS
CATCATACCAAGCTTCATGA+GGG 0.642119 8.4:+55474248 MS.gene74341:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8.4:+55475412 MS.gene74341:CDS
TGATGGTCATCTTGTCAAAG+GGG 0.652198 8.4:+55475544 MS.gene74341:CDS
TGACATAACAACAGATGGCG+AGG 0.663434 8.4:+55475286 MS.gene74341:intron
TGATTATGAGAAGAACAATG+AGG 0.694185 8.4:+55475466 MS.gene74341:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACTATAAAATAGTA+AGG + chr8.4:55475056-55475075 MS.gene74341:intron 10.0%
!! TTATCTTATTATAATATTTC+TGG + chr8.4:55475194-55475213 MS.gene74341:intron 10.0%
!!! TTATTTTACTAAAAACTTTA+AGG - chr8.4:55474961-55474980 None:intergenic 10.0%
!! AAATTCTAAATTAAGTCAAT+AGG - chr8.4:55474523-55474542 None:intergenic 15.0%
!! AGATAATCTATATAAAAAGT+AGG + chr8.4:55474894-55474913 MS.gene74341:intron 15.0%
!! GATAATCTATATAAAAAGTA+GGG + chr8.4:55474895-55474914 MS.gene74341:intron 15.0%
!! TATAGTAATAAAAAGAAGAT+TGG + chr8.4:55474409-55474428 MS.gene74341:intron 15.0%
!!! AAAGCTTATTTTATTCTTTT+TGG + chr8.4:55475250-55475269 MS.gene74341:intron 15.0%
!! CATCATATAAGATCTTTAAA+AGG + chr8.4:55475097-55475116 MS.gene74341:intron 20.0%
!!! GAATAAAATAAGCTTTTCAA+AGG - chr8.4:55475246-55475265 None:intergenic 20.0%
! AAAATCACATACAAGCTATT+TGG + chr8.4:55475356-55475375 MS.gene74341:CDS 25.0%
! AGTAATAAAAAGAAGATTGG+TGG + chr8.4:55474412-55474431 MS.gene74341:intron 25.0%
! GTAAACTTATTACTGAACTT+GGG + chr8.4:55474147-55474166 MS.gene74341:CDS 25.0%
! GTAATAAAAAGAAGATTGGT+GGG + chr8.4:55474413-55474432 MS.gene74341:intron 25.0%
! TATCAAACAAGATGATGATT+TGG - chr8.4:55474351-55474370 None:intergenic 25.0%
! TCTTCGTTATAGAATTCATT+TGG - chr8.4:55474449-55474468 None:intergenic 25.0%
! TGGAGATATTGAAAACTATA+AGG + chr8.4:55475379-55475398 MS.gene74341:CDS 25.0%
! TTATCTCTAGACAAAAAATC+TGG - chr8.4:55474880-55474899 None:intergenic 25.0%
!!! ATCTTTAAAAGGTGTTTTAG+AGG + chr8.4:55475108-55475127 MS.gene74341:intron 25.0%
AAAAAATTATACCCGTACCA+TGG - chr8.4:55474835-55474854 None:intergenic 30.0%
GGTAAACTTATTACTGAACT+TGG + chr8.4:55474146-55474165 MS.gene74341:CDS 30.0%
GTTTGTATGATATGATCCTT+CGG + chr8.4:55474917-55474936 MS.gene74341:intron 30.0%
TGATTATGAGAAGAACAATG+AGG + chr8.4:55475466-55475485 MS.gene74341:CDS 30.0%
AAACACTGGACTTATGTCAT+AGG + chr8.4:55474299-55474318 MS.gene74341:CDS 35.0%
AAAGCAACAAATGACCCTAA+AGG + chr8.4:55474777-55474796 MS.gene74341:intron 35.0%
AGTAGCATCTCTTTCCTTTA+GGG - chr8.4:55474794-55474813 None:intergenic 35.0%
ATCACATACAAGCTATTTGG+TGG + chr8.4:55475359-55475378 MS.gene74341:CDS 35.0%
ATGGAAATTCTGCTGTTAGA+TGG + chr8.4:55475438-55475457 MS.gene74341:CDS 35.0%
TATCGAAATCATTGTACACG+AGG + chr8.4:55474627-55474646 MS.gene74341:intron 35.0%
! AGATTGGCATCATACTGATT+CGG + chr8.4:55474274-55474293 MS.gene74341:CDS 35.0%
! ATTGATGGTCATCTTGTCAA+AGG + chr8.4:55475542-55475561 MS.gene74341:CDS 35.0%
! TTGATGGTCATCTTGTCAAA+GGG + chr8.4:55475543-55475562 MS.gene74341:CDS 35.0%
!! AAGAGCACTAGAGATATTGA+TGG + chr8.4:55475527-55475546 MS.gene74341:CDS 35.0%
!! ATACTGATTCGGTTAAACAC+TGG + chr8.4:55474285-55474304 MS.gene74341:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG + chr8.4:55475412-55475431 MS.gene74341:CDS 35.0%
AACCACCTTTGTATTCACCA+TGG + chr8.4:55474815-55474834 MS.gene74341:intron 40.0%
AGTTTGTAGAACTTGTCAGC+TGG - chr8.4:55474182-55474201 None:intergenic 40.0%
ATGTTGATACTCCAAATGGC+TGG + chr8.4:55475489-55475508 MS.gene74341:CDS 40.0%
CATCATACCAAGCTTCATGA+GGG + chr8.4:55474248-55474267 MS.gene74341:CDS 40.0%
CCTTTGTATTCACCATGGTA+CGG + chr8.4:55474820-55474839 MS.gene74341:intron 40.0%
CTTTGTATTCACCATGGTAC+GGG + chr8.4:55474821-55474840 MS.gene74341:intron 40.0%
GAGGATGTTGATACTCCAAA+TGG + chr8.4:55475485-55475504 MS.gene74341:CDS 40.0%
GAGTAGCATCTCTTTCCTTT+AGG - chr8.4:55474795-55474814 None:intergenic 40.0%
TACCATGGTGAATACAAAGG+TGG - chr8.4:55474820-55474839 None:intergenic 40.0%
TATCTCTAGTGCTCTTGCTA+AGG - chr8.4:55475523-55475542 None:intergenic 40.0%
TCATCATACCAAGCTTCATG+AGG + chr8.4:55474247-55474266 MS.gene74341:CDS 40.0%
TCATCTTGTCAAAGGGGAAA+AGG + chr8.4:55475550-55475569 MS.gene74341:CDS 40.0%
TGAGACTGATCTAACAACGA+AGG + chr8.4:55474486-55474505 MS.gene74341:intron 40.0%
TGATGGTCATCTTGTCAAAG+GGG + chr8.4:55475544-55475563 MS.gene74341:CDS 40.0%
! CGTCTTTAGACATTTTCCGA+AGG - chr8.4:55474936-55474955 None:intergenic 40.0%
AGCTTCATGAGGGTGAAGAT+TGG + chr8.4:55474258-55474277 MS.gene74341:CDS 45.0%
ATCTTCACCCTCATGAAGCT+TGG - chr8.4:55474258-55474277 None:intergenic 45.0%
CCGTACCATGGTGAATACAA+AGG - chr8.4:55474823-55474842 None:intergenic 45.0%
GCCATCTGTTGTTATGTCAG+AGG - chr8.4:55475285-55475304 None:intergenic 45.0%
GCCTCTGACATAACAACAGA+TGG + chr8.4:55475281-55475300 MS.gene74341:intron 45.0%
TGACATAACAACAGATGGCG+AGG + chr8.4:55475286-55475305 MS.gene74341:intron 45.0%
TGATACTCCAAATGGCTGGA+TGG + chr8.4:55475493-55475512 MS.gene74341:CDS 45.0%
TTGTACACGAGGTGAAGTAC+TGG + chr8.4:55474638-55474657 MS.gene74341:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG + chr8.4:55475419-55475438 MS.gene74341:CDS 45.0%
!! AAATAATATATATTATGAAT+GGG - chr8.4:55474732-55474751 None:intergenic 5.0%
!! AATATATATTATTTAGAAAA+AGG + chr8.4:55474738-55474757 MS.gene74341:intron 5.0%
!! AATTAATAAAAAAAAAGTAA+CGG - chr8.4:55474692-55474711 None:intergenic 5.0%
!! TAAATAATATATATTATGAA+TGG - chr8.4:55474733-55474752 None:intergenic 5.0%
!!! TAATTTAGAATTTTTTTAAT+TGG + chr8.4:55474530-55474549 MS.gene74341:intron 5.0%
AGGTAGTCCATCCAGCCATT+TGG - chr8.4:55475503-55475522 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 55474134 55475583 55474134 ID=MS.gene74341
chr8.4 mRNA 55474134 55475583 55474134 ID=MS.gene74341.t1;Parent=MS.gene74341
chr8.4 exon 55474134 55474320 55474134 ID=MS.gene74341.t1.exon1;Parent=MS.gene74341.t1
chr8.4 CDS 55474134 55474320 55474134 ID=cds.MS.gene74341.t1;Parent=MS.gene74341.t1
chr8.4 exon 55475300 55475583 55475300 ID=MS.gene74341.t1.exon2;Parent=MS.gene74341.t1
chr8.4 CDS 55475300 55475583 55475300 ID=cds.MS.gene74341.t1;Parent=MS.gene74341.t1
Gene Sequence

>MS.gene74341

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCAGCTGACAAGTTCTACAAACTATTTGCATCAGAACTTCATGAAGTGCAAAATGTTTGTGAAAGAGTTCATCATACCAAGCTTCATGAGGGTGAAGATTGGCATCATACTGATTCGGTTAAACACTGGACTTATGTCATAGGTAATTCTATTTCTAATGTTTCGATAACCAAATCATCATCTTGTTTGATATGAAAAAGTTTTCTATTACAAATTTTTTTAATGATAATTATAGTAATAAAAAGAAGATTGGTGGGTTAAAGAATACCAAATGAATTCTATAACGAAGAATAAAGATTTGATTCATTGAGACTGATCTAACAACGAAGGCAATATTTTAGCCTATTGACTTAATTTAGAATTTTTTTAATTGGTTAGAAAGTTTGTAAGAGTTTTAGATATATAAATTGTTAAAATAAAAAGTTAAATTCATGAAAAAAAATTGAGATATCGAAATCATTGTACACGAGGTGAAGTACTGGATCTCTAAAAATAGTACTTAAACTTTTTCCGTTACTTTTTTTTTATTAATTAAGAATGAGCTTGAACACCCATTCATAATATATATTATTTAGAAAAAGGAAGAGAAAAAAAATAAAAAGCAACAAATGACCCTAAAGGAAAGAGATGCTACTCAACCACCTTTGTATTCACCATGGTACGGGTATAATTTTTTCTTTACGAATGTAATTACATTTCCAGATTTTTTGTCTAGAGATAATCTATATAAAAAGTAGGGTTTGTATGATATGATCCTTCGGAAAATGTCTAAAGACGAACCTTAAAGTTTTTAGTAAAATAATGTATTTTATCATAATATTTTAAAAACAGATTAAATATGTTTTTTTACGAGAAAAACAGATTAAATATGTCACTAAAATTTACTATAAAATAGTAAGGTTCATACTAGATTAAATTCATCATATAAGATCTTTAAAAGGTGTTTTAGAGGCATTCGTTAGATAATGTAATATATCTTTTTTAATGCAGAAATAAATTAAAGCATCATTTATCATTATCTTATTATAATATTTCTGGTGCAACTAATCTTTATTATATTAATTCCTTTGAAAAGCTTATTTTATTCTTTTTGGTATTAATTGCCTCTGACATAACAACAGATGGCGAGGTACACACATGCAATGAGAGTGTTGAAGAAGTTGATGATCAGAACAAAAAAATCACATACAAGCTATTTGGTGGAGATATTGAAAACTATAAGGTATTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAATTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATGTTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAGAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MS.gene74341.t1

MVLAGKLITELGIKTPADKFYKLFASELHEVQNVCERVHHTKLHEGEDWHHTDSVKHWTYVIDGEVHTCNESVEEVDDQNKKITYKLFGGDIENYKVFKLILEVLNKADGNSAVRWTIDYEKNNEDVDTPNGWMDYLSKSTRDIDGHLVKGEKVAL