AlfalfaGEDB | MS.gene62988

M. sativa cultivar XinJiangDaYe / MS.gene62988

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62988.t1	RHN77962.1	92.8	153	11	0	1	153	5	157	2.80E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62988.t1	Q9SSK5	37.7	154	92	3	2	153	6	157	4.6e-26	119.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62988.t1	I3T9Y8	92.8	153	11	0	1	153	1	153	2.0e-75	291.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050496	MS.gene62988	-0.804557	2.04E-49	-1.69E-46
MS.gene050883	MS.gene62988	0.801465	8.89E-49	-1.69E-46
MS.gene051044	MS.gene62988	0.818985	1.47E-52	-1.69E-46
MS.gene051364	MS.gene62988	0.829306	5.54E-55	-1.69E-46
MS.gene051366	MS.gene62988	0.830864	2.31E-55	-1.69E-46
MS.gene051367	MS.gene62988	0.827667	1.38E-54	-1.69E-46
MS.gene051422	MS.gene62988	0.81436	1.60E-51	-1.69E-46
MS.gene055942	MS.gene62988	0.837323	5.56E-57	-1.69E-46
MS.gene058099	MS.gene62988	0.805965	1.03E-49	-1.69E-46
MS.gene058111	MS.gene62988	0.837872	4.02E-57	-1.69E-46
MS.gene058268	MS.gene62988	0.812799	3.53E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene59869	MS.gene62988	PPI
MS.gene016336	MS.gene62988	PPI
MS.gene74354	MS.gene62988	PPI
MS.gene016314	MS.gene62988	PPI
MS.gene62988	MS.gene77781	PPI
MS.gene016330	MS.gene62988	PPI
MS.gene77758	MS.gene62988	PPI
MS.gene99548	MS.gene62988	PPI
MS.gene066940	MS.gene62988	PPI
MS.gene62988	MS.gene74358	PPI
MS.gene62988	MS.gene83129	PPI
MS.gene62988	MS.gene74346	PPI
MS.gene62988	MS.gene026605	PPI
MS.gene62988	MS.gene59140	PPI
MS.gene058382	MS.gene62988	PPI
MS.gene77780	MS.gene62988	PPI
MS.gene74365	MS.gene62988	PPI
MS.gene77768	MS.gene62988	PPI
MS.gene62988	MS.gene74367	PPI
MS.gene59863	MS.gene62988	PPI
MS.gene59841	MS.gene62988	PPI
MS.gene77770	MS.gene62988	PPI
MS.gene62988	MS.gene59847	PPI
MS.gene62988	MS.gene59859	PPI
MS.gene74355	MS.gene62988	PPI
MS.gene62988	MS.gene058383	PPI
MS.gene62988	MS.gene016307	PPI
MS.gene74343	MS.gene62988	PPI
MS.gene77774	MS.gene62988	PPI
MS.gene77763	MS.gene62988	PPI
MS.gene62988	MS.gene77774	PPI
MS.gene62988	MS.gene59860	PPI
MS.gene59857	MS.gene62988	PPI
MS.gene74364	MS.gene62988	PPI
MS.gene016327	MS.gene62988	PPI
MS.gene74342	MS.gene62988	PPI
MS.gene59849	MS.gene62988	PPI
MS.gene058384	MS.gene62988	PPI
MS.gene59862	MS.gene62988	PPI
MS.gene62988	MS.gene016315	PPI
MS.gene62988	MS.gene59840	PPI
MS.gene74344	MS.gene62988	PPI
MS.gene62988	MS.gene058386	PPI
MS.gene77761	MS.gene62988	PPI
MS.gene016326	MS.gene62988	PPI
MS.gene62988	MS.gene016317	PPI
MS.gene77769	MS.gene62988	PPI
MS.gene016337	MS.gene62988	PPI
MS.gene74352	MS.gene62988	PPI
MS.gene74353	MS.gene62988	PPI
MS.gene77771	MS.gene62988	PPI
MS.gene62988	MS.gene74341	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62988.t1	MTR_1g030820	92.810	153	11	0	1	153	1	153	3.50e-101	287
MS.gene62988.t1	MTR_1g030810	86.928	153	20	0	1	153	1	153	1.37e-96	275
MS.gene62988.t1	MTR_1g030840	57.419	155	61	2	1	150	1	155	1.05e-59	182
MS.gene62988.t1	MTR_8g045490	40.523	153	88	2	1	152	1	151	1.43e-37	126
MS.gene62988.t1	MTR_8g045570	39.869	153	89	2	1	152	1	151	1.90e-37	125
MS.gene62988.t1	MTR_8g045400	39.216	153	89	3	1	152	1	150	6.50e-36	122
MS.gene62988.t1	MTR_8g045695	38.562	153	90	3	1	152	1	150	9.62e-36	121
MS.gene62988.t1	MTR_8g045735	38.562	153	90	3	1	152	1	150	1.43e-35	121
MS.gene62988.t1	MTR_8g045560	38.562	153	90	3	1	152	1	150	1.44e-35	121
MS.gene62988.t1	MTR_8g045555	36.601	153	94	2	1	152	1	151	3.49e-35	120
MS.gene62988.t1	MTR_8g045520	37.662	154	93	2	1	153	1	152	1.21e-34	119
MS.gene62988.t1	MTR_8g045665	37.255	153	92	3	1	152	1	150	7.60e-34	116
MS.gene62988.t1	MTR_8g045640	37.255	153	92	3	1	152	1	150	1.14e-33	116
MS.gene62988.t1	MTR_8g045300	38.961	154	91	2	1	153	1	152	1.58e-33	115
MS.gene62988.t1	MTR_1g031640	37.662	154	86	5	1	152	1	146	4.75e-28	101
MS.gene62988.t1	MTR_8g012550	35.897	156	94	4	1	153	1	153	6.32e-28	101

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene62988.t1	AT1G70890	37.662	154	92	3	2	153	6	157	6.20e-32	112
MS.gene62988.t1	AT5G28010	38.961	154	90	3	2	153	14	165	1.25e-31	111
MS.gene62988.t1	AT5G28010	38.961	154	90	3	2	153	14	165	1.25e-31	111
MS.gene62988.t1	AT1G70840	35.065	154	96	3	2	153	19	170	1.09e-30	109
MS.gene62988.t1	AT1G70830	37.013	154	93	3	2	153	21	172	3.16e-30	108
MS.gene62988.t1	AT1G70830	37.013	154	93	3	2	153	21	172	6.64e-30	108
MS.gene62988.t1	AT1G70830	37.013	154	93	3	2	153	21	172	3.17e-29	107
MS.gene62988.t1	AT1G70830	37.013	154	93	3	2	153	21	172	1.21e-28	107
MS.gene62988.t1	AT1G70830	36.364	154	94	3	2	153	183	334	2.53e-27	104
MS.gene62988.t1	AT1G70830	36.364	154	94	3	2	153	136	287	7.12e-28	104
MS.gene62988.t1	AT1G70830	36.458	96	58	2	59	153	32	125	8.99e-14	67.0
MS.gene62988.t1	AT1G70850	34.211	152	96	3	2	151	8	157	1.05e-26	100
MS.gene62988.t1	AT1G70850	34.211	152	96	3	2	151	8	157	4.32e-26	100
MS.gene62988.t1	AT1G70850	35.664	143	88	3	13	153	175	315	3.60e-25	98.2
MS.gene62988.t1	AT1G70850	34.211	152	96	3	2	151	8	157	4.32e-26	100
MS.gene62988.t1	AT1G70850	35.664	143	88	3	13	153	175	315	3.60e-25	98.2
MS.gene62988.t1	AT5G28000	33.766	154	98	3	2	153	12	163	4.45e-26	97.1
MS.gene62988.t1	AT1G23130	27.451	153	108	2	2	153	9	159	5.59e-25	94.4
MS.gene62988.t1	AT1G70880	32.468	154	100	3	2	153	7	158	8.03e-25	94.0
MS.gene62988.t1	AT1G14930	35.294	153	95	3	1	152	1	150	4.25e-24	91.7
MS.gene62988.t1	AT1G35260	31.293	147	96	3	7	151	6	149	1.33e-23	90.5
MS.gene62988.t1	AT1G70870	36.842	152	82	5	1	152	1	138	2.74e-23	89.4
MS.gene62988.t1	AT1G23120	31.373	153	99	3	1	153	1	147	9.23e-21	83.2
MS.gene62988.t1	AT1G23120	31.373	153	99	3	1	153	51	197	2.35e-20	83.2
MS.gene62988.t1	AT1G14950	30.065	153	103	3	1	152	1	150	2.48e-19	79.7
MS.gene62988.t1	AT4G14060	30.065	153	103	3	1	152	1	150	5.37e-19	78.6
MS.gene62988.t1	AT1G35310	30.282	142	95	3	13	153	12	150	4.28e-18	76.3
MS.gene62988.t1	AT1G14940	29.605	152	103	3	1	151	1	149	1.98e-17	74.7
MS.gene62988.t1	AT2G01520	29.412	153	104	3	1	152	1	150	2.17e-17	74.3
MS.gene62988.t1	AT1G24020	35.802	162	85	7	1	153	1	152	3.97e-17	73.9
MS.gene62988.t1	AT1G24020	35.802	162	85	7	1	153	1	152	3.97e-17	73.9
MS.gene62988.t1	AT1G30990	27.632	152	105	3	1	151	1	148	3.03e-16	71.6
MS.gene62988.t1	AT2G01530	29.412	153	104	3	1	152	1	150	9.75e-16	70.1
MS.gene62988.t1	AT1G14960	28.105	153	106	3	1	152	1	150	1.11e-15	70.1
MS.gene62988.t1	AT3G26460	28.758	153	106	2	1	152	1	151	1.21e-13	64.7
MS.gene62988.t1	AT3G26450	28.571	154	105	4	1	152	1	151	3.52e-13	63.5
MS.gene62988.t1	AT4G23670	29.412	153	104	3	1	152	1	150	1.29e-12	62.0
MS.gene62988.t1	AT4G23680	27.451	153	107	3	1	152	1	150	3.16e-12	60.8

Find 30 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTACTTCTGTGGTGATATT+AGG	0.304651	1.3:-16557921	None:intergenic
TGAGAGTTACAAGAGTTTAA+AGG	0.313337	1.3:+16558587	MS.gene62988:CDS
ATGGTGGTGGGATTGTTAAA+TGG	0.324878	1.3:+16558637	MS.gene62988:CDS
GAAGGTGACTGGGAAAATAT+TGG	0.418929	1.3:+16557960	MS.gene62988:CDS
ATACTTGGACCTTGCTGTAA+AGG	0.426075	1.3:+16558713	MS.gene62988:CDS
GCAAGGTCCAAGTATGAATC+AGG	0.441032	1.3:-16558705	None:intergenic
TGGTGGTGGGATTGTTAAAT+GGG	0.470252	1.3:+16558638	MS.gene62988:CDS
TGTATCTCCTGATTCATACT+TGG	0.511390	1.3:+16558698	MS.gene62988:CDS
CATGGCTTTAAGTGGGAAAG+TGG	0.512659	1.3:+16557827	None:intergenic
CGAATATGAGAAGCTAAAAG+AGG	0.515741	1.3:+16558665	MS.gene62988:CDS
AGAAGAGACATGGCTTTAAG+TGG	0.526395	1.3:+16557819	None:intergenic
GTAGCGTGCATGAAGGTGAC+TGG	0.529809	1.3:+16557949	MS.gene62988:CDS
ATTCATAAAGAACATGGTGG+TGG	0.537340	1.3:+16558624	MS.gene62988:CDS
ATATTGGTTCTATCAAGCAC+TGG	0.538736	1.3:+16557976	MS.gene62988:CDS
TTCATAAAGAACATGGTGGT+GGG	0.570285	1.3:+16558625	MS.gene62988:CDS
AATATCACCACAGAAGTACA+TGG	0.570466	1.3:+16557924	MS.gene62988:CDS
AAGCTAAAAGAGGAAACTAC+AGG	0.574676	1.3:+16558675	MS.gene62988:CDS
TATTGGTTCTATCAAGCACT+GGG	0.594586	1.3:+16557977	MS.gene62988:CDS
CAAGTGATTCATAAAGAACA+TGG	0.605539	1.3:+16558618	MS.gene62988:CDS
TATTGATTCTCATCTTGTCA+AGG	0.605627	1.3:+16558746	MS.gene62988:CDS
GGCTTTAAGTGGGAAAGTGG+AGG	0.606303	1.3:+16557830	MS.gene62988:CDS
CATGGAGGTAGCGTGCATGA+AGG	0.614089	1.3:+16557942	MS.gene62988:CDS
TAGCGTGCATGAAGGTGACT+GGG	0.617034	1.3:+16557950	MS.gene62988:CDS
AAGCACTGGGAATTTACAGT+AGG	0.622307	1.3:+16557990	MS.gene62988:CDS
GAAGAGACATGGCTTTAAGT+GGG	0.623024	1.3:+16557820	None:intergenic
GCTACCTCCATGTACTTCTG+TGG	0.632806	1.3:-16557931	None:intergenic
GAAGTAGAGATTCAAGCACC+TGG	0.664321	1.3:+16557855	MS.gene62988:CDS
ATATTATAGAACTTAGCACC+AGG	0.680852	1.3:-16557873	None:intergenic
GTGATTCATAAAGAACATGG+TGG	0.735641	1.3:+16558621	MS.gene62988:CDS
ATCACCACAGAAGTACATGG+AGG	0.776492	1.3:+16557927	MS.gene62988:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAAATATGTTTTGTTTACAT+TGG	+	chr1.3:16558404-16558423	MS.gene62988:intron	15.0%
!!	AGTGTTTATAAATGACAATT+TGG	-	chr1.3:16558215-16558234	None:intergenic	20.0%
!!	TAGTAAAACTATGAAACATA+AGG	-	chr1.3:16558026-16558045	None:intergenic	20.0%
!!	TCATTTGCTTAAAAAAAACA+AGG	+	chr1.3:16558361-16558380	MS.gene62988:intron	20.0%
!!!	ATCATATATAGCATTTTTGA+TGG	+	chr1.3:16558555-16558574	MS.gene62988:CDS	20.0%
!!!	TGAGAAAGTTTTTTTTTTTG+TGG	-	chr1.3:16558252-16558271	None:intergenic	20.0%
!!!	TTATTTATTTTCCCTAATCA+TGG	+	chr1.3:16558441-16558460	MS.gene62988:intron	20.0%
!	GAATTTACAGTAGGTAATTA+AGG	+	chr1.3:16557999-16558018	MS.gene62988:intron	25.0%
!	TAAATCAAGTTCCATGATTA+GGG	-	chr1.3:16558455-16558474	None:intergenic	25.0%
!	TTAAATCAAGTTCCATGATT+AGG	-	chr1.3:16558456-16558475	None:intergenic	25.0%
!!	ACTATTTTATTGCTCATGAA+AGG	+	chr1.3:16558042-16558061	MS.gene62988:intron	25.0%
	ATATTATAGAACTTAGCACC+AGG	-	chr1.3:16557876-16557895	None:intergenic	30.0%
	CAAGTGATTCATAAAGAACA+TGG	+	chr1.3:16558618-16558637	MS.gene62988:CDS	30.0%
	TGAGAGTTACAAGAGTTTAA+AGG	+	chr1.3:16558587-16558606	MS.gene62988:CDS	30.0%
!	TAACATCAACAAAAGAGTGT+TGG	-	chr1.3:16558129-16558148	None:intergenic	30.0%
!!	TATTGATTCTCATCTTGTCA+AGG	+	chr1.3:16558746-16558765	MS.gene62988:CDS	30.0%
!!	TTTGTTTTCTTCCTTCTGTA+GGG	-	chr1.3:16558491-16558510	None:intergenic	30.0%
	AAGCTAAAAGAGGAAACTAC+AGG	+	chr1.3:16558675-16558694	MS.gene62988:CDS	35.0%
	ATTCATAAAGAACATGGTGG+TGG	+	chr1.3:16558624-16558643	MS.gene62988:CDS	35.0%
	CGAATATGAGAAGCTAAAAG+AGG	+	chr1.3:16558665-16558684	MS.gene62988:CDS	35.0%
	GTGATTCATAAAGAACATGG+TGG	+	chr1.3:16558621-16558640	MS.gene62988:CDS	35.0%
	TGTACTTCTGTGGTGATATT+AGG	-	chr1.3:16557924-16557943	None:intergenic	35.0%
	TGTATCTCCTGATTCATACT+TGG	+	chr1.3:16558698-16558717	MS.gene62988:CDS	35.0%
	TTCATAAAGAACATGGTGGT+GGG	+	chr1.3:16558625-16558644	MS.gene62988:CDS	35.0%
!	AATATCACCACAGAAGTACA+TGG	+	chr1.3:16557924-16557943	MS.gene62988:CDS	35.0%
!	AGCATTTTTGATGGAGAAGT+GGG	+	chr1.3:16558564-16558583	MS.gene62988:CDS	35.0%
!	TAGCATTTTTGATGGAGAAG+TGG	+	chr1.3:16558563-16558582	MS.gene62988:CDS	35.0%
!!	ATATTGGTTCTATCAAGCAC+TGG	+	chr1.3:16557976-16557995	MS.gene62988:CDS	35.0%
!!	ATCAACAAAAGAGTGTTGGA+AGG	-	chr1.3:16558125-16558144	None:intergenic	35.0%
!!	TATTGGTTCTATCAAGCACT+GGG	+	chr1.3:16557977-16557996	MS.gene62988:CDS	35.0%
!!!	CTTTGTTTTCTTCCTTCTGT+AGG	-	chr1.3:16558492-16558511	None:intergenic	35.0%
	GAAGGTGACTGGGAAAATAT+TGG	+	chr1.3:16557960-16557979	MS.gene62988:CDS	40.0%
	TACTTGTGAAACCCTACAGA+AGG	+	chr1.3:16558477-16558496	MS.gene62988:intron	40.0%
!	ATACTTGGACCTTGCTGTAA+AGG	+	chr1.3:16558713-16558732	MS.gene62988:CDS	40.0%
!	CTTTTGTGACCTTTACAGCA+AGG	-	chr1.3:16558725-16558744	None:intergenic	40.0%
!!	AACAAAAGAGTGTTGGAAGG+AGG	-	chr1.3:16558122-16558141	None:intergenic	40.0%
!!	AAGCACTGGGAATTTACAGT+AGG	+	chr1.3:16557990-16558009	MS.gene62988:CDS	40.0%
!!	ACAAAAGAGTGTTGGAAGGA+GGG	-	chr1.3:16558121-16558140	None:intergenic	40.0%
!!	ATGGTGGTGGGATTGTTAAA+TGG	+	chr1.3:16558637-16558656	MS.gene62988:CDS	40.0%
!!	TGGTGGTGGGATTGTTAAAT+GGG	+	chr1.3:16558638-16558657	MS.gene62988:CDS	40.0%
	GAAGTAGAGATTCAAGCACC+TGG	+	chr1.3:16557855-16557874	MS.gene62988:CDS	45.0%
	GCAAGGTCCAAGTATGAATC+AGG	-	chr1.3:16558708-16558727	None:intergenic	45.0%
!	ATCACCACAGAAGTACATGG+AGG	+	chr1.3:16557927-16557946	MS.gene62988:CDS	45.0%
	GCTACCTCCATGTACTTCTG+TGG	-	chr1.3:16557934-16557953	None:intergenic	50.0%
	TAGCGTGCATGAAGGTGACT+GGG	+	chr1.3:16557950-16557969	MS.gene62988:CDS	50.0%
!!	GGCTTTAAGTGGGAAAGTGG+AGG	+	chr1.3:16557830-16557849	MS.gene62988:CDS	50.0%
	CATGGAGGTAGCGTGCATGA+AGG	+	chr1.3:16557942-16557961	MS.gene62988:CDS	55.0%
	GTAGCGTGCATGAAGGTGAC+TGG	+	chr1.3:16557949-16557968	MS.gene62988:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	16557828	16558773	16557828	ID=MS.gene62988
chr1.3	mRNA	16557828	16558773	16557828	ID=MS.gene62988.t1;Parent=MS.gene62988
chr1.3	exon	16557828	16558011	16557828	ID=MS.gene62988.t1.exon1;Parent=MS.gene62988.t1
chr1.3	CDS	16557828	16558011	16557828	ID=cds.MS.gene62988.t1;Parent=MS.gene62988.t1
chr1.3	exon	16558496	16558773	16558496	ID=MS.gene62988.t1.exon2;Parent=MS.gene62988.t1
chr1.3	CDS	16558496	16558773	16558496	ID=cds.MS.gene62988.t1;Parent=MS.gene62988.t1

Gene Sequence

>MS.gene62988

ATGGCTTTAAGTGGGAAAGTGGAGGCTGAAGTAGAGATTCAAGCACCTGGTGCTAAGTTCTATAATATTTTCAGAAAGCAACTAGAACATCTTCCTAATATCACCACAGAAGTACATGGAGGTAGCGTGCATGAAGGTGACTGGGAAAATATTGGTTCTATCAAGCACTGGGAATTTACAGTAGGTAATTAAGGTCCTTATGTTTCATAGTTTTACTATTTTATTGCTCATGAAAGGTAGAGTACATCAATGAACGCACAAAAAAGAATATATTAAAAAGTATTAATGAACCCTCCTTCCAACACTCTTTTGTTGATGTTAATAAACTTTACATGATTTGATTATCAGCAAAAATAATTCATTCAAAATTTTAGCTTGTATTCTCCAAATTGTCATTTATAAACACTTGATCAATGATGTTCCACAAAAAAAAAAACTTTCTCAATTAAAATAAAATATTAAAAGTATTATTAAGAAAATAAAATATATACTCTTTTTTCTTTTTCAATTTTTATTTTTAAATATTTTGTTTTTCATTTGCTTAAAAAAAACAAGGTATTTCAAAAATAAAATCGTTAAATATGTTTTGTTTACATTGGTCTCTAATATCTATTTATTTATTTTCCCTAATCATGGAACTTGATTTAAATACTTGTGAAACCCTACAGAAGGAAGAAAACAAAGTGCTAAAGCAAAAATTGAGACTATAGATGATGACAATAAGATAATCATATATAGCATTTTTGATGGAGAAGTGGGTGAGAGTTACAAGAGTTTAAAGGCAATATTACAAGTGATTCATAAAGAACATGGTGGTGGGATTGTTAAATGGGCATACGAATATGAGAAGCTAAAAGAGGAAACTACAGGTGTATCTCCTGATTCATACTTGGACCTTGCTGTAAAGGTCACAAAAGATATTGATTCTCATCTTGTCAAGGAATAG

Protein sequence

>MS.gene62988.t1

MALSGKVEAEVEIQAPGAKFYNIFRKQLEHLPNITTEVHGGSVHEGDWENIGSIKHWEFTVEGRKQSAKAKIETIDDDNKIIIYSIFDGEVGESYKSLKAILQVIHKEHGGGIVKWAYEYEKLKEETTGVSPDSYLDLAVKVTKDIDSHLVKE