AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77768


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77768.t1 MTR_8g045520 93.631 157 10 0 1 157 1 157 7.82e-108 304
MS.gene77768.t1 MTR_8g045490 80.255 157 30 1 1 157 1 156 6.48e-91 261
MS.gene77768.t1 MTR_8g045695 79.618 157 31 1 1 157 1 156 1.38e-89 258
MS.gene77768.t1 MTR_8g045400 78.981 157 32 1 1 157 1 156 2.12e-89 257
MS.gene77768.t1 MTR_8g045555 75.497 151 37 0 1 151 1 151 1.46e-85 248
MS.gene77768.t1 MTR_8g045665 76.433 157 36 1 1 157 1 156 5.94e-85 246
MS.gene77768.t1 MTR_8g045300 74.359 156 40 0 1 156 1 156 8.92e-85 246
MS.gene77768.t1 MTR_8g045570 75.497 151 37 0 1 151 1 151 1.91e-84 244
MS.gene77768.t1 MTR_8g045640 75.159 157 38 1 1 157 1 156 1.21e-83 243
MS.gene77768.t1 MTR_8g045735 74.194 155 39 1 1 155 1 154 3.71e-83 241
MS.gene77768.t1 MTR_8g045560 72.611 157 42 1 1 157 1 156 2.02e-80 234
MS.gene77768.t1 MTR_1g030810 41.558 154 87 2 1 152 1 153 2.27e-38 128
MS.gene77768.t1 MTR_1g030820 37.662 154 93 2 1 152 1 153 7.22e-37 124
MS.gene77768.t1 MTR_1g030840 35.897 156 92 4 1 149 1 155 2.37e-27 100
MS.gene77768.t1 MTR_8g012550 31.333 150 100 3 6 154 8 155 3.60e-25 94.7
MS.gene77768.t1 MTR_1g031640 34.694 147 87 5 7 151 7 146 8.89e-24 90.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77768.t1 AT5G28010 34.211 152 97 3 3 152 15 165 2.21e-26 98.2
MS.gene77768.t1 AT5G28010 34.211 152 97 3 3 152 15 165 2.21e-26 98.2
MS.gene77768.t1 AT1G70890 36.184 152 94 3 3 152 7 157 4.39e-26 97.1
MS.gene77768.t1 AT1G70840 32.895 152 99 3 3 152 20 170 8.84e-24 91.7
MS.gene77768.t1 AT1G23120 31.944 144 93 3 10 153 10 148 1.62e-23 90.1
MS.gene77768.t1 AT1G70830 32.895 152 99 3 3 152 22 172 6.21e-23 89.4
MS.gene77768.t1 AT1G23120 31.944 144 93 3 10 153 60 198 6.98e-23 90.1
MS.gene77768.t1 AT5G28000 31.579 152 101 3 3 152 13 163 1.05e-22 88.6
MS.gene77768.t1 AT1G70830 32.895 152 99 3 3 152 22 172 1.17e-22 89.4
MS.gene77768.t1 AT1G70870 36.424 151 83 4 1 151 1 138 3.26e-22 86.7
MS.gene77768.t1 AT1G70830 32.895 152 99 3 3 152 22 172 4.30e-22 89.0
MS.gene77768.t1 AT1G70830 32.895 152 99 3 3 152 137 287 8.98e-22 89.0
MS.gene77768.t1 AT1G70830 28.421 95 67 1 59 152 31 125 5.31e-11 59.7
MS.gene77768.t1 AT1G70830 32.895 152 99 3 3 152 22 172 1.11e-21 89.4
MS.gene77768.t1 AT1G70830 32.895 152 99 3 3 152 184 334 2.59e-21 88.6
MS.gene77768.t1 AT1G70850 32.450 151 99 3 3 151 9 158 1.50e-21 87.4
MS.gene77768.t1 AT1G70850 32.450 151 99 3 3 151 9 158 4.20e-21 87.8
MS.gene77768.t1 AT1G70850 33.099 142 92 3 13 152 175 315 5.11e-21 87.4
MS.gene77768.t1 AT1G70850 32.450 151 99 3 3 151 9 158 4.20e-21 87.8
MS.gene77768.t1 AT1G70850 33.099 142 92 3 13 152 175 315 5.11e-21 87.4
MS.gene77768.t1 AT1G24020 32.468 154 100 3 1 152 1 152 1.21e-18 77.8
MS.gene77768.t1 AT1G24020 32.468 154 100 3 1 152 1 152 1.21e-18 77.8
MS.gene77768.t1 AT1G23130 27.152 151 108 2 3 152 10 159 1.79e-18 77.4
MS.gene77768.t1 AT4G23670 34.194 155 93 4 1 151 1 150 2.11e-18 77.0
MS.gene77768.t1 AT1G70880 28.289 152 106 3 3 152 8 158 3.90e-18 76.6
MS.gene77768.t1 AT1G14930 30.263 152 103 3 1 151 1 150 1.27e-17 75.1
MS.gene77768.t1 AT1G35260 28.082 146 101 3 9 152 8 151 4.33e-16 71.2
MS.gene77768.t1 AT4G23680 30.323 155 99 4 1 151 1 150 9.30e-16 70.5
MS.gene77768.t1 AT2G01520 28.947 152 105 3 1 151 1 150 1.01e-15 70.1
MS.gene77768.t1 AT1G35310 26.056 142 102 3 13 153 12 151 3.38e-14 66.2
MS.gene77768.t1 AT4G14060 29.677 155 100 4 1 151 1 150 4.40e-14 65.9
MS.gene77768.t1 AT1G14940 29.139 151 104 3 1 150 1 149 5.66e-14 65.9
MS.gene77768.t1 AT1G14950 28.750 160 105 4 1 156 1 155 1.49e-13 64.7
MS.gene77768.t1 AT1G30990 30.464 151 101 4 1 150 1 148 1.60e-13 64.3
MS.gene77768.t1 AT2G01530 27.632 152 107 3 1 151 1 150 4.99e-13 63.2
MS.gene77768.t1 AT3G26460 30.380 158 96 6 1 151 1 151 8.22e-11 57.4

Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGTACTTACAAGCTCTT+TGG 0.306715 8.1:+59892485 MS.gene77768:CDS
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8.1:+59892570 MS.gene77768:CDS
AAGGTACTTACAAGCTCTTT+GGG 0.349654 8.1:+59892486 MS.gene77768:CDS
CACCCTTATCAATTACTTCA+AGG 0.382574 8.1:-59892547 None:intergenic
AGATATTGATGAGCACTATA+AGG 0.414096 8.1:+59892511 MS.gene77768:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8.1:+59891661 MS.gene77768:CDS
CATCCTTGAAGTAATTGATA+AGG 0.438738 8.1:+59892544 MS.gene77768:CDS
ATCTTCACCCTCATGCAGCT+TGG 0.445602 8.1:-59891770 None:intergenic
AAGTAGTCCATCCAGCCATT+TGG 0.456227 8.1:-59892632 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 8.1:+59891814 MS.gene77768:CDS
ATCCTTGAAGTAATTGATAA+GGG 0.486541 8.1:+59892545 MS.gene77768:CDS
TGAAATAACAACAGATGGTA+AGG 0.489687 8.1:+59892415 MS.gene77768:intron
AGCTGCATGAGGGTGAAGAT+TGG 0.493681 8.1:+59891773 MS.gene77768:CDS
TATGACTTATAGAGCAACCT+TGG 0.515175 8.1:-59892699 None:intergenic
TGATGCTCATCTTGTCAAGC+AGG 0.523708 8.1:+59892676 MS.gene77768:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 8.1:+59892621 MS.gene77768:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 8.1:+59892617 MS.gene77768:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8.1:+59891662 MS.gene77768:CDS
ATTGATGAACAACACAAGAA+AGG 0.543540 8.1:+59892467 MS.gene77768:CDS
AGATTGGCATCACACTGATT+CGG 0.545548 8.1:+59891789 MS.gene77768:CDS
GAAGTAATTGATAAGGGTGA+TGG 0.549119 8.1:+59892551 MS.gene77768:CDS
TAGTCCATCCAGCCATTTGG+AGG 0.551025 8.1:-59892629 None:intergenic
ACACTGATTCGGTCAAACAC+TGG 0.556043 8.1:+59891800 MS.gene77768:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 8.1:+59892625 MS.gene77768:CDS
GGATCAAAACACCCGCTGAA+AGG 0.578575 8.1:+59891683 MS.gene77768:CDS
TCATCTTGTCAAGCAGGCCA+AGG 0.591246 8.1:+59892682 MS.gene77768:CDS
TCATGAAACCAAGCTGCATG+AGG 0.602712 8.1:+59891762 MS.gene77768:CDS
AGGTACTTACAAGCTCTTTG+GGG 0.621914 8.1:+59892487 MS.gene77768:CDS
CATGAAACCAAGCTGCATGA+GGG 0.630684 8.1:+59891763 MS.gene77768:CDS
AGTTTGAAGAACCTTTCAGC+GGG 0.631322 8.1:-59891694 None:intergenic
GGTACTTACAAGCTCTTTGG+GGG 0.640678 8.1:+59892488 MS.gene77768:CDS
GGTACACACATGTCATGAGA+AGG 0.648648 8.1:+59892436 MS.gene77768:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.649199 8.1:-59892628 None:intergenic
TAGTTTGAAGAACCTTTCAG+CGG 0.662028 8.1:-59891695 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 8.1:+59892598 MS.gene77768:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATTTGTCTAATA+TGG + chr8.1:59892231-59892250 MS.gene77768:intron 10.0%
!! AATCTTACTAAAAAATGTTA+AGG - chr8.1:59892354-59892373 None:intergenic 15.0%
!! TAGACAAATATTATTATTGA+TGG - chr8.1:59892228-59892247 None:intergenic 15.0%
!! AATTAAAGAGATGTACAAAA+AGG + chr8.1:59892323-59892342 MS.gene77768:intron 20.0%
!! ACAAATATTATTATTGATGG+TGG - chr8.1:59892225-59892244 None:intergenic 20.0%
!! CTTACTAAAAAATTGTACAA+CGG - chr8.1:59891986-59892005 None:intergenic 20.0%
!!! GATCTCTAAAAAATGTTTAA+GGG + chr8.1:59892253-59892272 MS.gene77768:intron 20.0%
! AGCAAAAAATTGTACAATAC+TGG - chr8.1:59892189-59892208 None:intergenic 25.0%
! ATCCTTGAAGTAATTGATAA+GGG + chr8.1:59892545-59892564 MS.gene77768:CDS 25.0%
! GATCCTTATTAAAAATGGAA+AGG - chr8.1:59892167-59892186 None:intergenic 25.0%
! GTAAACTTATTACTGAACTT+GGG + chr8.1:59891662-59891681 MS.gene77768:CDS 25.0%
! TACTGGATCCTTATTAAAAA+TGG - chr8.1:59892172-59892191 None:intergenic 25.0%
! TGTTGTACTACAAATGATAA+CGG - chr8.1:59892283-59892302 None:intergenic 25.0%
!! GGATCTCTAAAAAATGTTTA+AGG + chr8.1:59892252-59892271 MS.gene77768:intron 25.0%
!!! CTAAAACTGCTGTTATTTTT+TGG + chr8.1:59892379-59892398 MS.gene77768:intron 25.0%
!!! GATCCTTTCCATTTTTAATA+AGG + chr8.1:59892161-59892180 MS.gene77768:intron 25.0%
AGATATTGATGAGCACTATA+AGG + chr8.1:59892511-59892530 MS.gene77768:CDS 30.0%
ATTGATGAACAACACAAGAA+AGG + chr8.1:59892467-59892486 MS.gene77768:CDS 30.0%
CATCCTTGAAGTAATTGATA+AGG + chr8.1:59892544-59892563 MS.gene77768:CDS 30.0%
GGTAAACTTATTACTGAACT+TGG + chr8.1:59891661-59891680 MS.gene77768:CDS 30.0%
TGAAATAACAACAGATGGTA+AGG + chr8.1:59892415-59892434 MS.gene77768:intron 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8.1:59892598-59892617 MS.gene77768:CDS 30.0%
!! ACCATCTGTTGTTATTTCAT+GGG - chr8.1:59892414-59892433 None:intergenic 30.0%
!! TACCATCTGTTGTTATTTCA+TGG - chr8.1:59892415-59892434 None:intergenic 30.0%
AAACACTGGACTTATGTCAT+AGG + chr8.1:59891814-59891833 MS.gene77768:CDS 35.0%
CAAAATCATCTTGTTGGACT+TGG + chr8.1:59891869-59891888 MS.gene77768:intron 35.0%
CACCCTTATCAATTACTTCA+AGG - chr8.1:59892550-59892569 None:intergenic 35.0%
GAAGTAATTGATAAGGGTGA+TGG + chr8.1:59892551-59892570 MS.gene77768:CDS 35.0%
TAGTTTGAAGAACCTTTCAG+CGG - chr8.1:59891698-59891717 None:intergenic 35.0%
TGCCAACAAAATCATCTTGT+TGG + chr8.1:59891863-59891882 MS.gene77768:intron 35.0%
! AAGGTACTTACAAGCTCTTT+GGG + chr8.1:59892486-59892505 MS.gene77768:CDS 35.0%
! GTCCAACAAGATGATTTTGT+TGG - chr8.1:59891868-59891887 None:intergenic 35.0%
!! AAAGGTACTTACAAGCTCTT+TGG + chr8.1:59892485-59892504 MS.gene77768:CDS 35.0%
AGGTACTTACAAGCTCTTTG+GGG + chr8.1:59892487-59892506 MS.gene77768:CDS 40.0%
AGTTTGAAGAACCTTTCAGC+GGG - chr8.1:59891697-59891716 None:intergenic 40.0%
GCCCATGAAATAACAACAGA+TGG + chr8.1:59892410-59892429 MS.gene77768:intron 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8.1:59891789-59891808 MS.gene77768:CDS 40.0%
AAGTAGTCCATCCAGCCATT+TGG - chr8.1:59892635-59892654 None:intergenic 45.0%
ATATTGACCCTCCAAATGGC+TGG + chr8.1:59892621-59892640 MS.gene77768:CDS 45.0%
CATGAAACCAAGCTGCATGA+GGG + chr8.1:59891763-59891782 MS.gene77768:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8.1:59892617-59892636 MS.gene77768:CDS 45.0%
GGTACACACATGTCATGAGA+AGG + chr8.1:59892436-59892455 MS.gene77768:CDS 45.0%
GGTACTTACAAGCTCTTTGG+GGG + chr8.1:59892488-59892507 MS.gene77768:CDS 45.0%
TCATGAAACCAAGCTGCATG+AGG + chr8.1:59891762-59891781 MS.gene77768:CDS 45.0%
TGATGCTCATCTTGTCAAGC+AGG + chr8.1:59892676-59892695 MS.gene77768:CDS 45.0%
!! ACACTGATTCGGTCAAACAC+TGG + chr8.1:59891800-59891819 MS.gene77768:CDS 45.0%
AGCTGCATGAGGGTGAAGAT+TGG + chr8.1:59891773-59891792 MS.gene77768:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr8.1:59892631-59892650 None:intergenic 50.0%
ATCTTCACCCTCATGCAGCT+TGG - chr8.1:59891773-59891792 None:intergenic 50.0%
ATGGCACTGCTGCTGCTAAA+TGG + chr8.1:59892570-59892589 MS.gene77768:CDS 50.0%
GGATCAAAACACCCGCTGAA+AGG + chr8.1:59891683-59891702 MS.gene77768:CDS 50.0%
TAGTCCATCCAGCCATTTGG+AGG - chr8.1:59892632-59892651 None:intergenic 50.0%
TCATCTTGTCAAGCAGGCCA+AGG + chr8.1:59892682-59892701 MS.gene77768:CDS 50.0%
TGACCCTCCAAATGGCTGGA+TGG + chr8.1:59892625-59892644 MS.gene77768:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 59891649 59892715 59891649 ID=MS.gene77768
chr8.1 mRNA 59891649 59892715 59891649 ID=MS.gene77768.t1;Parent=MS.gene77768
chr8.1 exon 59891649 59891835 59891649 ID=MS.gene77768.t1.exon1;Parent=MS.gene77768.t1
chr8.1 CDS 59891649 59891835 59891649 ID=cds.MS.gene77768.t1;Parent=MS.gene77768.t1
chr8.1 exon 59892429 59892715 59892429 ID=MS.gene77768.t1.exon2;Parent=MS.gene77768.t1
chr8.1 CDS 59892429 59892715 59892429 ID=cds.MS.gene77768.t1;Parent=MS.gene77768.t1
Gene Sequence

>MS.gene77768

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCCGCTGAAAGGTTCTTCAAACTATTTGCATCAGAACTTCATGAAGTGCAAAAACACTGTGAAAGAGTTCATGAAACCAAGCTGCATGAGGGTGAAGATTGGCATCACACTGATTCGGTCAAACACTGGACTTATGTCATAGGTAATTATATTTCTAACTAATTTAACTTGCCAACAAAATCATCTTGTTGGACTTGGATTTTTCTATCATTTAAGTTTATTTACGTGTTGATCACATTACTTAGTTTAGTTTATTATGTTTCATCACAAAAATAAAGCTTCAAGATAGCCGTTGTACAATTTTTTAGTAAGTTTAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAAGATATGTAAAAAAGCAGATCCTTTCCATTTTTAATAAGGATCCAGTATTGTACAATTTTTTGCTGTTTATATTAGATCCACCATCAATAATAATATTTGTCTAATATGGATCTCTAAAAAATGTTTAAGGGTGAACCGTTATCATTTGTAGTACAACAATGAATTTAATAATTAAAAAAATTAAAGAGATGTACAAAAAGGTAGATCCTTAACATTTTTTAGTAAGATTGCACTCTAAAACTGCTGTTATTTTTTGGTATTAATTGCCCATGAAATAACAACAGATGGTAAGGTACACACATGTCATGAGAAGGTTGAAGAAATTGATGAACAACACAAGAAAGGTACTTACAAGCTCTTTGGGGGAGATATTGATGAGCACTATAAGGACTTTAAGCTCATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTACTTATGCAAATGTACTCGAGATATTGATGCTCATCTTGTCAAGCAGGCCAAGGTTGCTCTATAA

Protein sequence

>MS.gene77768.t1

MVLAGKLITELGIKTPAERFFKLFASELHEVQKHCERVHETKLHEGEDWHHTDSVKHWTYVIDGKVHTCHEKVEEIDEQHKKGTYKLFGGDIDEHYKDFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGWMDYLCKCTRDIDAHLVKQAKVAL