AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74352


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74352.t1 MTR_8g045520 94.268 157 9 0 1 157 1 157 5.37e-109 307
MS.gene74352.t1 MTR_8g045490 80.892 157 29 1 1 157 1 156 6.84e-92 264
MS.gene74352.t1 MTR_8g045695 80.255 157 30 1 1 157 1 156 2.09e-90 260
MS.gene74352.t1 MTR_8g045400 79.618 157 31 1 1 157 1 156 2.88e-90 259
MS.gene74352.t1 MTR_8g045555 76.159 151 36 0 1 151 1 151 1.73e-86 250
MS.gene74352.t1 MTR_8g045665 77.070 157 35 1 1 157 1 156 9.11e-86 248
MS.gene74352.t1 MTR_8g045300 75.000 156 39 0 1 156 1 156 1.09e-85 248
MS.gene74352.t1 MTR_8g045570 76.159 151 36 0 1 151 1 151 2.18e-85 247
MS.gene74352.t1 MTR_8g045640 75.796 157 37 1 1 157 1 156 2.05e-84 244
MS.gene74352.t1 MTR_8g045735 74.839 155 38 1 1 155 1 154 5.63e-84 244
MS.gene74352.t1 MTR_8g045560 73.248 157 41 1 1 157 1 156 3.39e-81 236
MS.gene74352.t1 MTR_1g030810 42.208 154 86 2 1 152 1 153 3.23e-39 130
MS.gene74352.t1 MTR_1g030820 38.312 154 92 2 1 152 1 153 1.02e-37 126
MS.gene74352.t1 MTR_1g030840 36.538 156 91 4 1 149 1 155 4.35e-28 102
MS.gene74352.t1 MTR_8g012550 32.000 150 99 3 6 154 8 155 6.68e-26 96.7
MS.gene74352.t1 MTR_1g031640 35.374 147 86 5 7 151 7 146 2.54e-24 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74352.t1 AT5G28010 34.211 152 97 3 3 152 15 165 1.30e-26 98.6
MS.gene74352.t1 AT5G28010 34.211 152 97 3 3 152 15 165 1.30e-26 98.6
MS.gene74352.t1 AT1G70890 36.184 152 94 3 3 152 7 157 2.51e-26 97.8
MS.gene74352.t1 AT1G23120 32.639 144 92 3 10 153 10 148 3.47e-24 92.0
MS.gene74352.t1 AT1G70840 32.895 152 99 3 3 152 20 170 4.45e-24 92.4
MS.gene74352.t1 AT1G23120 32.639 144 92 3 10 153 60 198 1.51e-23 91.7
MS.gene74352.t1 AT1G70830 32.895 152 99 3 3 152 22 172 3.52e-23 90.1
MS.gene74352.t1 AT1G70830 32.895 152 99 3 3 152 22 172 6.63e-23 90.1
MS.gene74352.t1 AT1G70870 37.086 151 82 4 1 151 1 138 8.12e-23 88.2
MS.gene74352.t1 AT5G28000 31.579 152 101 3 3 152 13 163 9.40e-23 89.0
MS.gene74352.t1 AT1G70830 32.895 152 99 3 3 152 22 172 2.56e-22 89.7
MS.gene74352.t1 AT1G70830 32.895 152 99 3 3 152 137 287 5.23e-22 89.7
MS.gene74352.t1 AT1G70830 28.421 95 67 1 59 152 31 125 3.31e-11 60.1
MS.gene74352.t1 AT1G70830 32.895 152 99 3 3 152 22 172 6.44e-22 90.1
MS.gene74352.t1 AT1G70830 32.895 152 99 3 3 152 184 334 1.57e-21 89.0
MS.gene74352.t1 AT1G70850 32.450 151 99 3 3 151 9 158 7.96e-22 88.2
MS.gene74352.t1 AT1G70850 32.450 151 99 3 3 151 9 158 2.31e-21 88.2
MS.gene74352.t1 AT1G70850 33.099 142 92 3 13 152 175 315 2.96e-21 88.2
MS.gene74352.t1 AT1G70850 32.450 151 99 3 3 151 9 158 2.31e-21 88.2
MS.gene74352.t1 AT1G70850 33.099 142 92 3 13 152 175 315 2.96e-21 88.2
MS.gene74352.t1 AT1G24020 33.117 154 99 3 1 152 1 152 1.98e-19 80.1
MS.gene74352.t1 AT1G24020 33.117 154 99 3 1 152 1 152 1.98e-19 80.1
MS.gene74352.t1 AT4G23670 34.839 155 92 4 1 151 1 150 2.44e-19 79.7
MS.gene74352.t1 AT1G23130 27.152 151 108 2 3 152 10 159 1.49e-18 77.8
MS.gene74352.t1 AT1G70880 28.289 152 106 3 3 152 8 158 2.92e-18 77.0
MS.gene74352.t1 AT1G14930 30.263 152 103 3 1 151 1 150 1.13e-17 75.5
MS.gene74352.t1 AT2G01520 29.605 152 104 3 1 151 1 150 1.51e-16 72.4
MS.gene74352.t1 AT1G35260 28.082 146 101 3 9 152 8 151 4.15e-16 71.2
MS.gene74352.t1 AT4G23680 30.323 155 99 4 1 151 1 150 4.61e-16 71.2
MS.gene74352.t1 AT4G14060 30.323 155 99 4 1 151 1 150 6.21e-15 68.2
MS.gene74352.t1 AT1G14940 29.139 151 104 3 1 150 1 149 4.58e-14 65.9
MS.gene74352.t1 AT1G35310 26.056 142 102 3 13 153 12 151 4.84e-14 65.9
MS.gene74352.t1 AT2G01530 28.289 152 106 3 1 151 1 150 6.94e-14 65.5
MS.gene74352.t1 AT1G14950 28.750 160 105 4 1 156 1 155 1.12e-13 65.1
MS.gene74352.t1 AT1G30990 30.464 151 101 4 1 150 1 148 1.36e-13 64.7
MS.gene74352.t1 AT3G26460 31.013 158 95 6 1 151 1 151 1.35e-11 59.3
MS.gene74352.t1 AT1G14960 26.797 153 109 3 1 152 1 151 7.94e-11 57.4

Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGTACTTACAAGCTCTT+TGG 0.306715 8.4:+55663497 MS.gene74352:CDS
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8.4:+55663582 MS.gene74352:CDS
AAGGTACTTACAAGCTCTTT+GGG 0.349654 8.4:+55663498 MS.gene74352:CDS
CACCCTTATCAATTACTTCA+AGG 0.382574 8.4:-55663559 None:intergenic
AGATATTGATGAGCACTATA+AGG 0.414096 8.4:+55663523 MS.gene74352:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8.4:+55662756 MS.gene74352:CDS
CATCCTTGAAGTAATTGATA+AGG 0.438738 8.4:+55663556 MS.gene74352:CDS
ATCTTCACCCTCATGCAGCT+TGG 0.445602 8.4:-55662865 None:intergenic
AAGTAGTCCATCCAGCCATT+TGG 0.456227 8.4:-55663644 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 8.4:+55662909 MS.gene74352:CDS
ATCCTTGAAGTAATTGATAA+GGG 0.486541 8.4:+55663557 MS.gene74352:CDS
TGAAATAACAACAGATGGTA+AGG 0.489687 8.4:+55663427 MS.gene74352:intron
AGCTGCATGAGGGTGAAGAT+TGG 0.493681 8.4:+55662868 MS.gene74352:CDS
TATGACTTATAGAGCAACCT+TGG 0.515175 8.4:-55663711 None:intergenic
TGATGCTCATCTTGTCAAGC+AGG 0.523708 8.4:+55663688 MS.gene74352:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 8.4:+55663633 MS.gene74352:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 8.4:+55663629 MS.gene74352:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8.4:+55662757 MS.gene74352:CDS
AGATTGGCATCACACTGATT+CGG 0.545548 8.4:+55662884 MS.gene74352:CDS
ATTGATGAACAAAACAAGAA+AGG 0.549109 8.4:+55663479 MS.gene74352:CDS
GAAGTAATTGATAAGGGTGA+TGG 0.549119 8.4:+55663563 MS.gene74352:CDS
TAGTCCATCCAGCCATTTGG+AGG 0.551025 8.4:-55663641 None:intergenic
ACACTGATTCGGTCAAACAC+TGG 0.556043 8.4:+55662895 MS.gene74352:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 8.4:+55663637 MS.gene74352:CDS
GGATCAAAACACCCGCTGAA+AGG 0.578575 8.4:+55662778 MS.gene74352:CDS
TCATCTTGTCAAGCAGGCCA+AGG 0.591246 8.4:+55663694 MS.gene74352:CDS
TCATGAAACCAAGCTGCATG+AGG 0.602712 8.4:+55662857 MS.gene74352:CDS
AGGTACTTACAAGCTCTTTG+GGG 0.621914 8.4:+55663499 MS.gene74352:CDS
CATGAAACCAAGCTGCATGA+GGG 0.630684 8.4:+55662858 MS.gene74352:CDS
AGTTTGAAGAACCTTTCAGC+GGG 0.631322 8.4:-55662789 None:intergenic
GGTACTTACAAGCTCTTTGG+GGG 0.640678 8.4:+55663500 MS.gene74352:CDS
GGTACACACATGTCATGAGA+AGG 0.648648 8.4:+55663448 MS.gene74352:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.649199 8.4:-55663640 None:intergenic
TAGTTTGAAGAACCTTTCAG+CGG 0.662028 8.4:-55662790 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 8.4:+55663610 MS.gene74352:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATTTGTCTAATA+TGG + chr8.4:55663238-55663257 MS.gene74352:intron 10.0%
!! AATCTTACTAAAAAATGTTA+AGG - chr8.4:55663361-55663380 None:intergenic 15.0%
!! TAGACAAATATTATTATTGA+TGG - chr8.4:55663235-55663254 None:intergenic 15.0%
!!! TATTTTATTGAAAATAGCAA+CGG - chr8.4:55663081-55663100 None:intergenic 15.0%
!! AATTAAAGAGATGTACAAAA+AGG + chr8.4:55663330-55663349 MS.gene74352:intron 20.0%
!! ACAAATATTATTATTGATGG+TGG - chr8.4:55663232-55663251 None:intergenic 20.0%
!!! ACTGCTGTTATTTTTTTTTT+TGG + chr8.4:55663391-55663410 MS.gene74352:intron 20.0%
!!! GATCTCTAAAAAATGTTTAA+GGG + chr8.4:55663260-55663279 MS.gene74352:intron 20.0%
! AGCAAAAAATTGTACAATAC+TGG - chr8.4:55663196-55663215 None:intergenic 25.0%
! ATCCTTGAAGTAATTGATAA+GGG + chr8.4:55663557-55663576 MS.gene74352:CDS 25.0%
! ATTGATGAACAAAACAAGAA+AGG + chr8.4:55663479-55663498 MS.gene74352:CDS 25.0%
! GATCCTTATTAAAAATGGAA+AGG - chr8.4:55663174-55663193 None:intergenic 25.0%
! GTAAACTTATTACTGAACTT+GGG + chr8.4:55662757-55662776 MS.gene74352:CDS 25.0%
! TACTGGATCCTTATTAAAAA+TGG - chr8.4:55663179-55663198 None:intergenic 25.0%
! TGTTGTACTACAAATGATAA+CGG - chr8.4:55663290-55663309 None:intergenic 25.0%
!! GGATCTCTAAAAAATGTTTA+AGG + chr8.4:55663259-55663278 MS.gene74352:intron 25.0%
!!! GATCCTTTCCATTTTTAATA+AGG + chr8.4:55663168-55663187 MS.gene74352:intron 25.0%
AGATATTGATGAGCACTATA+AGG + chr8.4:55663523-55663542 MS.gene74352:CDS 30.0%
CATCCTTGAAGTAATTGATA+AGG + chr8.4:55663556-55663575 MS.gene74352:CDS 30.0%
GGTAAACTTATTACTGAACT+TGG + chr8.4:55662756-55662775 MS.gene74352:CDS 30.0%
TGAAATAACAACAGATGGTA+AGG + chr8.4:55663427-55663446 MS.gene74352:intron 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8.4:55663610-55663629 MS.gene74352:CDS 30.0%
!! ACCATCTGTTGTTATTTCAT+GGG - chr8.4:55663426-55663445 None:intergenic 30.0%
!! TACCATCTGTTGTTATTTCA+TGG - chr8.4:55663427-55663446 None:intergenic 30.0%
AAACACTGGACTTATGTCAT+AGG + chr8.4:55662909-55662928 MS.gene74352:CDS 35.0%
CAAAATCATCTTGTTGGACT+TGG + chr8.4:55662964-55662983 MS.gene74352:intron 35.0%
CACCCTTATCAATTACTTCA+AGG - chr8.4:55663562-55663581 None:intergenic 35.0%
GAAGTAATTGATAAGGGTGA+TGG + chr8.4:55663563-55663582 MS.gene74352:CDS 35.0%
TAGTTTGAAGAACCTTTCAG+CGG - chr8.4:55662793-55662812 None:intergenic 35.0%
TGCCAACAAAATCATCTTGT+TGG + chr8.4:55662958-55662977 MS.gene74352:intron 35.0%
! AAGGTACTTACAAGCTCTTT+GGG + chr8.4:55663498-55663517 MS.gene74352:CDS 35.0%
! GTCCAACAAGATGATTTTGT+TGG - chr8.4:55662963-55662982 None:intergenic 35.0%
!! AAAGGTACTTACAAGCTCTT+TGG + chr8.4:55663497-55663516 MS.gene74352:CDS 35.0%
AGGTACTTACAAGCTCTTTG+GGG + chr8.4:55663499-55663518 MS.gene74352:CDS 40.0%
AGTTTGAAGAACCTTTCAGC+GGG - chr8.4:55662792-55662811 None:intergenic 40.0%
GCCCATGAAATAACAACAGA+TGG + chr8.4:55663422-55663441 MS.gene74352:intron 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8.4:55662884-55662903 MS.gene74352:CDS 40.0%
AAGTAGTCCATCCAGCCATT+TGG - chr8.4:55663647-55663666 None:intergenic 45.0%
ATATTGACCCTCCAAATGGC+TGG + chr8.4:55663633-55663652 MS.gene74352:CDS 45.0%
CATGAAACCAAGCTGCATGA+GGG + chr8.4:55662858-55662877 MS.gene74352:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8.4:55663629-55663648 MS.gene74352:CDS 45.0%
GGTACACACATGTCATGAGA+AGG + chr8.4:55663448-55663467 MS.gene74352:CDS 45.0%
GGTACTTACAAGCTCTTTGG+GGG + chr8.4:55663500-55663519 MS.gene74352:CDS 45.0%
TCATGAAACCAAGCTGCATG+AGG + chr8.4:55662857-55662876 MS.gene74352:CDS 45.0%
TGATGCTCATCTTGTCAAGC+AGG + chr8.4:55663688-55663707 MS.gene74352:CDS 45.0%
!! ACACTGATTCGGTCAAACAC+TGG + chr8.4:55662895-55662914 MS.gene74352:CDS 45.0%
AGCTGCATGAGGGTGAAGAT+TGG + chr8.4:55662868-55662887 MS.gene74352:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG - chr8.4:55663643-55663662 None:intergenic 50.0%
ATCTTCACCCTCATGCAGCT+TGG - chr8.4:55662868-55662887 None:intergenic 50.0%
ATGGCACTGCTGCTGCTAAA+TGG + chr8.4:55663582-55663601 MS.gene74352:CDS 50.0%
GGATCAAAACACCCGCTGAA+AGG + chr8.4:55662778-55662797 MS.gene74352:CDS 50.0%
TAGTCCATCCAGCCATTTGG+AGG - chr8.4:55663644-55663663 None:intergenic 50.0%
TCATCTTGTCAAGCAGGCCA+AGG + chr8.4:55663694-55663713 MS.gene74352:CDS 50.0%
TGACCCTCCAAATGGCTGGA+TGG + chr8.4:55663637-55663656 MS.gene74352:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 55662744 55663727 55662744 ID=MS.gene74352
chr8.4 mRNA 55662744 55663727 55662744 ID=MS.gene74352.t1;Parent=MS.gene74352
chr8.4 exon 55662744 55662930 55662744 ID=MS.gene74352.t1.exon1;Parent=MS.gene74352.t1
chr8.4 CDS 55662744 55662930 55662744 ID=cds.MS.gene74352.t1;Parent=MS.gene74352.t1
chr8.4 exon 55663441 55663727 55663441 ID=MS.gene74352.t1.exon2;Parent=MS.gene74352.t1
chr8.4 CDS 55663441 55663727 55663441 ID=cds.MS.gene74352.t1;Parent=MS.gene74352.t1
Gene Sequence

>MS.gene74352

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCCGCTGAAAGGTTCTTCAAACTATTTGCATCAGAACTTCATGAAGTGCAAAAACACTGTGAAAGAGTTCATGAAACCAAGCTGCATGAGGGTGAAGATTGGCATCACACTGATTCGGTCAAACACTGGACTTATGTCATAGGTAATTATATTTCTAACTAATTTAACTTGCCAACAAAATCATCTTGTTGGACTTGGATTTTTCTATCATTTAAGTTTATTTACGTGTTGATCACATTACTTAGTTTAGTTTATTATGTTTCATCACAAAAATAAAGCTTCAAGATAGCCGTTGCTATTTTCAATAAAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAAGATATGTAAAAAAGCAGATCCTTTCCATTTTTAATAAGGATCCAGTATTGTACAATTTTTTGCTGTTTATATTAGATCCACCATCAATAATAATATTTGTCTAATATGGATCTCTAAAAAATGTTTAAGGGTGAACCGTTATCATTTGTAGTACAACAATGAATTTAATAATTAAAAAAATTAAAGAGATGTACAAAAAGGTAGATCCTTAACATTTTTTAGTAAGATTGCACTCTAAAACTGCTGTTATTTTTTTTTTTGGTATTAATTGCCCATGAAATAACAACAGATGGTAAGGTACACACATGTCATGAGAAGGTTGAAGAAATTGATGAACAAAACAAGAAAGGTACTTACAAGCTCTTTGGGGGAGATATTGATGAGCACTATAAGGACTTTAAGCTCATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTACTTATGCAAATGTACTCGAGATATTGATGCTCATCTTGTCAAGCAGGCCAAGGTTGCTCTATAA

Protein sequence

>MS.gene74352.t1

MVLAGKLITELGIKTPAERFFKLFASELHEVQKHCERVHETKLHEGEDWHHTDSVKHWTYVIDGKVHTCHEKVEEIDEQNKKGTYKLFGGDIDEHYKDFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGWMDYLCKCTRDIDAHLVKQAKVAL