AlfalfaGEDB | MS.gene74352

M. sativa cultivar XinJiangDaYe / MS.gene74352

KEGG: -
GO: GO:0006950 GO:0006952 GO:0008150 GO:0050896
PFAM: PF00407.22

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74352.t1	XP_003628202.1	94.3	157	9	0	1	157	1	157	6.80E-85	323.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74352.t1	Q9SSK5	36.2	152	94	3	3	152	7	157	7.1e-22	105.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74352.t1	B7FHH7	94.3	157	9	0	1	157	1	157	4.9e-85	323.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene74352	-0.834048	3.75E-56	-1.69E-46
MS.gene050323	MS.gene74352	-0.81333	2.70E-51	-1.69E-46
MS.gene050326	MS.gene74352	0.836769	7.71E-57	-1.69E-46
MS.gene050328	MS.gene74352	0.84086	6.74E-58	-1.69E-46
MS.gene050338	MS.gene74352	0.842551	2.41E-58	-1.69E-46
MS.gene050496	MS.gene74352	-0.830208	3.34E-55	-1.69E-46
MS.gene051364	MS.gene74352	0.801122	1.04E-48	-1.69E-46
MS.gene051422	MS.gene74352	0.810047	1.40E-50	-1.69E-46
MS.gene051651	MS.gene74352	0.803309	3.71E-49	-1.69E-46
MS.gene051718	MS.gene74352	0.80739	5.17E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene016327	MS.gene74352	PPI
MS.gene016337	MS.gene74352	PPI
MS.gene058382	MS.gene74352	PPI
MS.gene058384	MS.gene74352	PPI
MS.gene066940	MS.gene74352	PPI
MS.gene59841	MS.gene74352	PPI
MS.gene59849	MS.gene74352	PPI
MS.gene59857	MS.gene74352	PPI
MS.gene59862	MS.gene74352	PPI
MS.gene59863	MS.gene74352	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74352.t1	MTR_8g045520	94.268	157	9	0	1	157	1	157	5.37e-109	307
MS.gene74352.t1	MTR_8g045490	80.892	157	29	1	1	157	1	156	6.84e-92	264
MS.gene74352.t1	MTR_8g045695	80.255	157	30	1	1	157	1	156	2.09e-90	260
MS.gene74352.t1	MTR_8g045400	79.618	157	31	1	1	157	1	156	2.88e-90	259
MS.gene74352.t1	MTR_8g045555	76.159	151	36	0	1	151	1	151	1.73e-86	250
MS.gene74352.t1	MTR_8g045665	77.070	157	35	1	1	157	1	156	9.11e-86	248
MS.gene74352.t1	MTR_8g045300	75.000	156	39	0	1	156	1	156	1.09e-85	248
MS.gene74352.t1	MTR_8g045570	76.159	151	36	0	1	151	1	151	2.18e-85	247
MS.gene74352.t1	MTR_8g045640	75.796	157	37	1	1	157	1	156	2.05e-84	244
MS.gene74352.t1	MTR_8g045735	74.839	155	38	1	1	155	1	154	5.63e-84	244

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene74352.t1	AT5G28010	34.211	152	97	3	3	152	15	165	1.30e-26	98.6
MS.gene74352.t1	AT5G28010	34.211	152	97	3	3	152	15	165	1.30e-26	98.6
MS.gene74352.t1	AT1G70890	36.184	152	94	3	3	152	7	157	2.51e-26	97.8
MS.gene74352.t1	AT1G23120	32.639	144	92	3	10	153	10	148	3.47e-24	92.0
MS.gene74352.t1	AT1G70840	32.895	152	99	3	3	152	20	170	4.45e-24	92.4
MS.gene74352.t1	AT1G23120	32.639	144	92	3	10	153	60	198	1.51e-23	91.7
MS.gene74352.t1	AT1G70830	32.895	152	99	3	3	152	22	172	3.52e-23	90.1
MS.gene74352.t1	AT1G70830	32.895	152	99	3	3	152	22	172	6.63e-23	90.1
MS.gene74352.t1	AT1G70870	37.086	151	82	4	1	151	1	138	8.12e-23	88.2
MS.gene74352.t1	AT5G28000	31.579	152	101	3	3	152	13	163	9.40e-23	89.0

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Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTGGACTTATGTCAT+AGG	0.470664	8.4:+55662909	MS.gene74352:CDS
AAAGGTACTTACAAGCTCTT+TGG	0.306715	8.4:+55663497	MS.gene74352:CDS
AAGGTACTTACAAGCTCTTT+GGG	0.349654	8.4:+55663498	MS.gene74352:CDS
AAGTAGTCCATCCAGCCATT+TGG	0.456227	8.4:-55663644	None:intergenic
ACACTGATTCGGTCAAACAC+TGG	0.556043	8.4:+55662895	MS.gene74352:CDS
AGATATTGATGAGCACTATA+AGG	0.414096	8.4:+55663523	MS.gene74352:CDS
AGATTGGCATCACACTGATT+CGG	0.545548	8.4:+55662884	MS.gene74352:CDS
AGCTGCATGAGGGTGAAGAT+TGG	0.493681	8.4:+55662868	MS.gene74352:CDS
AGGTACTTACAAGCTCTTTG+GGG	0.621914	8.4:+55663499	MS.gene74352:CDS
AGTCCATCCAGCCATTTGGA+GGG	0.649199	8.4:-55663640	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGATATTGATGAGCACTATA+AGG	+	chr8.4:55663523-55663542	MS.gene74352:CDS	30.0%
CATCCTTGAAGTAATTGATA+AGG	+	chr8.4:55663556-55663575	MS.gene74352:CDS	30.0%
GGTAAACTTATTACTGAACT+TGG	+	chr8.4:55662756-55662775	MS.gene74352:CDS	30.0%
TGAAATAACAACAGATGGTA+AGG	+	chr8.4:55663427-55663446	MS.gene74352:intron	30.0%
TGAATATGAGAAGATCAATG+AGG	+	chr8.4:55663610-55663629	MS.gene74352:CDS	30.0%
AAACACTGGACTTATGTCAT+AGG	+	chr8.4:55662909-55662928	MS.gene74352:CDS	35.0%
CAAAATCATCTTGTTGGACT+TGG	+	chr8.4:55662964-55662983	MS.gene74352:intron	35.0%
CACCCTTATCAATTACTTCA+AGG	-	chr8.4:55663562-55663581	None:intergenic	35.0%
GAAGTAATTGATAAGGGTGA+TGG	+	chr8.4:55663563-55663582	MS.gene74352:CDS	35.0%
TAGTTTGAAGAACCTTTCAG+CGG	-	chr8.4:55662793-55662812	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	55662744	55663727	55662744	ID=MS.gene74352
chr8.4	mRNA	55662744	55663727	55662744	ID=MS.gene74352.t1;Parent=MS.gene74352
chr8.4	exon	55662744	55662930	55662744	ID=MS.gene74352.t1.exon1;Parent=MS.gene74352.t1
chr8.4	CDS	55662744	55662930	55662744	ID=cds.MS.gene74352.t1;Parent=MS.gene74352.t1
chr8.4	exon	55663441	55663727	55663441	ID=MS.gene74352.t1.exon2;Parent=MS.gene74352.t1
chr8.4	CDS	55663441	55663727	55663441	ID=cds.MS.gene74352.t1;Parent=MS.gene74352.t1

Gene Sequence

>MS.gene74352

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCCGCTGAAAGGTTCTTCAAACTATTTGCATCAGAACTTCATGAAGTGCAAAAACACTGTGAAAGAGTTCATGAAACCAAGCTGCATGAGGGTGAAGATTGGCATCACACTGATTCGGTCAAACACTGGACTTATGTCATAGGTAATTATATTTCTAACTAATTTAACTTGCCAACAAAATCATCTTGTTGGACTTGGATTTTTCTATCATTTAAGTTTATTTACGTGTTGATCACATTACTTAGTTTAGTTTATTATGTTTCATCACAAAAATAAAGCTTCAAGATAGCCGTTGCTATTTTCAATAAAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAAAAGATATGTAAAAAAGCAGATCCTTTCCATTTTTAATAAGGATCCAGTATTGTACAATTTTTTGCTGTTTATATTAGATCCACCATCAATAATAATATTTGTCTAATATGGATCTCTAAAAAATGTTTAAGGGTGAACCGTTATCATTTGTAGTACAACAATGAATTTAATAATTAAAAAAATTAAAGAGATGTACAAAAAGGTAGATCCTTAACATTTTTTAGTAAGATTGCACTCTAAAACTGCTGTTATTTTTTTTTTTGGTATTAATTGCCCATGAAATAACAACAGATGGTAAGGTACACACATGTCATGAGAAGGTTGAAGAAATTGATGAACAAAACAAGAAAGGTACTTACAAGCTCTTTGGGGGAGATATTGATGAGCACTATAAGGACTTTAAGCTCATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTACTTATGCAAATGTACTCGAGATATTGATGCTCATCTTGTCAAGCAGGCCAAGGTTGCTCTATAA

Protein sequence

>MS.gene74352.t1

MVLAGKLITELGIKTPAERFFKLFASELHEVQKHCERVHETKLHEGEDWHHTDSVKHWTYVIDGKVHTCHEKVEEIDEQNKKGTYKLFGGDIDEHYKDFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGWMDYLCKCTRDIDAHLVKQAKVAL