AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83129


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83129.t1 MTR_1g030820 94.118 153 9 0 1 153 1 153 6.59e-103 291
MS.gene83129.t1 MTR_1g030810 87.582 153 19 0 1 153 1 153 1.43e-97 278
MS.gene83129.t1 MTR_1g030840 58.710 155 59 2 1 150 1 155 2.44e-61 186
MS.gene83129.t1 MTR_8g045490 41.176 153 87 2 1 152 1 151 1.06e-38 129
MS.gene83129.t1 MTR_8g045570 40.523 153 88 2 1 152 1 151 1.60e-38 128
MS.gene83129.t1 MTR_8g045400 39.869 153 88 3 1 152 1 150 4.47e-37 125
MS.gene83129.t1 MTR_8g045695 39.216 153 89 3 1 152 1 150 6.77e-37 124
MS.gene83129.t1 MTR_8g045560 39.216 153 89 3 1 152 1 150 1.30e-36 124
MS.gene83129.t1 MTR_8g045735 38.562 153 90 3 1 152 1 150 2.14e-36 123
MS.gene83129.t1 MTR_8g045555 37.255 153 93 2 1 152 1 151 2.27e-36 123
MS.gene83129.t1 MTR_8g045520 38.312 154 92 2 1 153 1 152 8.59e-36 121
MS.gene83129.t1 MTR_8g045300 39.610 154 90 2 1 153 1 152 1.05e-34 119
MS.gene83129.t1 MTR_8g045640 37.908 153 91 3 1 152 1 150 1.21e-34 118
MS.gene83129.t1 MTR_8g045665 37.255 153 92 3 1 152 1 150 1.34e-34 118
MS.gene83129.t1 MTR_8g012550 35.897 156 94 4 1 153 1 153 2.44e-28 102
MS.gene83129.t1 MTR_1g031640 37.013 154 87 5 1 152 1 146 3.41e-27 99.4
MS.gene83129.t1 MTR_5g035010 28.758 153 102 5 1 150 3 151 1.51e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83129.t1 AT1G70890 37.662 154 92 3 2 153 6 157 2.03e-32 113
MS.gene83129.t1 AT5G28010 38.961 154 90 3 2 153 14 165 1.01e-31 111
MS.gene83129.t1 AT5G28010 38.961 154 90 3 2 153 14 165 1.01e-31 111
MS.gene83129.t1 AT1G70840 35.065 154 96 3 2 153 19 170 5.29e-31 110
MS.gene83129.t1 AT1G70830 37.013 154 93 3 2 153 21 172 1.60e-30 108
MS.gene83129.t1 AT1G70830 37.013 154 93 3 2 153 21 172 3.02e-30 108
MS.gene83129.t1 AT1G70830 37.013 154 93 3 2 153 21 172 1.71e-29 108
MS.gene83129.t1 AT1G70830 37.013 154 93 3 2 153 21 172 6.38e-29 108
MS.gene83129.t1 AT1G70830 36.364 154 94 3 2 153 183 334 1.47e-27 105
MS.gene83129.t1 AT1G70830 36.364 154 94 3 2 153 136 287 4.38e-28 105
MS.gene83129.t1 AT1G70830 36.458 96 58 2 59 153 32 125 4.10e-14 68.2
MS.gene83129.t1 AT1G70850 34.211 152 96 3 2 151 8 157 5.78e-27 101
MS.gene83129.t1 AT5G28000 33.766 154 98 3 2 153 12 163 2.54e-26 97.8
MS.gene83129.t1 AT1G70850 34.211 152 96 3 2 151 8 157 2.70e-26 101
MS.gene83129.t1 AT1G70850 35.664 143 88 3 13 153 175 315 2.20e-25 99.0
MS.gene83129.t1 AT1G70850 34.211 152 96 3 2 151 8 157 2.70e-26 101
MS.gene83129.t1 AT1G70850 35.664 143 88 3 13 153 175 315 2.20e-25 99.0
MS.gene83129.t1 AT1G70880 32.468 154 100 3 2 153 7 158 2.22e-25 95.1
MS.gene83129.t1 AT1G23130 27.451 153 108 2 2 153 9 159 6.86e-25 94.0
MS.gene83129.t1 AT1G35260 31.293 147 96 3 7 151 6 149 1.45e-23 90.5
MS.gene83129.t1 AT1G14930 35.294 153 95 3 1 152 1 150 3.59e-23 89.4
MS.gene83129.t1 AT1G70870 36.842 152 82 5 1 152 1 138 6.43e-23 88.2
MS.gene83129.t1 AT1G23120 31.373 153 99 3 1 153 1 147 2.83e-21 84.3
MS.gene83129.t1 AT1G23120 31.373 153 99 3 1 153 51 197 8.38e-21 84.3
MS.gene83129.t1 AT1G14950 30.065 153 103 3 1 152 1 150 1.29e-18 77.8
MS.gene83129.t1 AT4G14060 30.065 153 103 3 1 152 1 150 2.91e-18 76.6
MS.gene83129.t1 AT1G35310 30.282 142 95 3 13 153 12 150 3.01e-18 76.6
MS.gene83129.t1 AT1G24020 36.420 162 84 7 1 153 1 152 6.20e-18 75.9
MS.gene83129.t1 AT1G24020 36.420 162 84 7 1 153 1 152 6.20e-18 75.9
MS.gene83129.t1 AT2G01520 29.412 153 104 3 1 152 1 150 2.61e-17 74.3
MS.gene83129.t1 AT1G14940 29.605 152 103 3 1 151 1 149 1.09e-16 72.8
MS.gene83129.t1 AT1G30990 27.632 152 105 3 1 151 1 148 7.97e-16 70.5
MS.gene83129.t1 AT2G01530 29.412 153 104 3 1 152 1 150 4.65e-15 68.2
MS.gene83129.t1 AT1G14960 28.105 153 106 3 1 152 1 150 7.03e-15 67.8
MS.gene83129.t1 AT3G26460 28.105 153 107 2 1 152 1 151 2.60e-13 63.9
MS.gene83129.t1 AT3G26450 28.571 154 105 4 1 152 1 151 4.49e-13 63.2
MS.gene83129.t1 AT4G23670 29.412 153 104 3 1 152 1 150 7.62e-13 62.4

Find 31 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGACTTCTGTGGTGATATT+AGG 0.304651 1.2:-16222535 None:intergenic
TGAGAGTTACAAGAGTTTAA+AGG 0.313337 1.2:+16223407 MS.gene83129:CDS
ATGGTGGTGGGATTGTTAAA+TGG 0.324878 1.2:+16223457 MS.gene83129:CDS
GAAGGTGACTGGGAAAATAT+TGG 0.418929 1.2:+16222574 MS.gene83129:CDS
ATACTTGGACCTTGCTGTAA+AGG 0.426075 1.2:+16223533 MS.gene83129:CDS
AAGCTAAAGGAGGAAATTAC+AGG 0.430297 1.2:+16223495 MS.gene83129:CDS
CGTATCTCCTGATTCATACT+TGG 0.435164 1.2:+16223518 MS.gene83129:CDS
GCAAGGTCCAAGTATGAATC+AGG 0.441032 1.2:-16223525 None:intergenic
TGGTGGTGGGATTGTTAAAT+GGG 0.470252 1.2:+16223458 MS.gene83129:CDS
AGAAGAGACATGGCTTTAAG+TGG 0.509760 1.2:+16222433 None:intergenic
CATGGCTTTAAGTGGGAAAG+TGG 0.512659 1.2:+16222441 None:intergenic
GTAGAGTGCATGAAGGTGAC+TGG 0.523294 1.2:+16222563 MS.gene83129:CDS
ATTCATAAAGAACATGGTGG+TGG 0.537340 1.2:+16223444 MS.gene83129:CDS
ATATTGGTTCTATCAAGCAC+TGG 0.538736 1.2:+16222590 MS.gene83129:CDS
CTTCATGCACTCTACCTCCA+TGG 0.564742 1.2:-16222555 None:intergenic
TTCATAAAGAACATGGTGGT+GGG 0.570285 1.2:+16223445 MS.gene83129:CDS
GAAGAGACATGGCTTTAAGT+GGG 0.591992 1.2:+16222434 None:intergenic
TATTGGTTCTATCAAGCACT+GGG 0.594586 1.2:+16222591 MS.gene83129:CDS
AATATCACCACAGAAGTCCA+TGG 0.603582 1.2:+16222538 MS.gene83129:CDS
TAGAGTGCATGAAGGTGACT+GGG 0.605256 1.2:+16222564 MS.gene83129:CDS
CAAGTGATTCATAAAGAACA+TGG 0.605539 1.2:+16223438 MS.gene83129:CDS
TATTGATTCTCATCTTGTCA+AGG 0.605627 1.2:+16223566 MS.gene83129:CDS
TCTACCTCCATGGACTTCTG+TGG 0.609185 1.2:-16222545 None:intergenic
ATACGAATATGAGAAGCTAA+AGG 0.610623 1.2:+16223482 MS.gene83129:CDS
AAGCACTGGGAATTTACAGT+AGG 0.622307 1.2:+16222604 MS.gene83129:CDS
CGAATATGAGAAGCTAAAGG+AGG 0.640547 1.2:+16223485 MS.gene83129:CDS
CATGGAGGTAGAGTGCATGA+AGG 0.659264 1.2:+16222556 MS.gene83129:CDS
GAAGTAGAGATTCAAGCACC+TGG 0.664321 1.2:+16222469 MS.gene83129:CDS
ATATTATAGAACTTAGCACC+AGG 0.680852 1.2:-16222487 None:intergenic
GTGATTCATAAAGAACATGG+TGG 0.735641 1.2:+16223441 MS.gene83129:CDS
ATCACCACAGAAGTCCATGG+AGG 0.764074 1.2:+16222541 MS.gene83129:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAGCTTAAATAATATTTT+AGG - chr1.2:16222946-16222965 None:intergenic 10.0%
!!! AAAGCTTAAATAATATTTTA+GGG - chr1.2:16222945-16222964 None:intergenic 10.0%
!!! AAATAAAAGATTAAAAGTAT+AGG + chr1.2:16222868-16222887 MS.gene83129:intron 10.0%
!!! AATAAAAGATTAAAAGTATA+GGG + chr1.2:16222869-16222888 MS.gene83129:intron 10.0%
!!! TAAAATATTATTTAAGCTTT+TGG + chr1.2:16222945-16222964 MS.gene83129:intron 10.0%
!! AATAAGCAAATGAAAAAAAA+AGG - chr1.2:16223170-16223189 None:intergenic 15.0%
!! ATTTGCTTATTAAAAAAACA+AGG + chr1.2:16223179-16223198 MS.gene83129:intron 15.0%
!!! AAGCTTAAATAATATTTTAG+GGG - chr1.2:16222944-16222963 None:intergenic 15.0%
!!! ATTTCATGAATGAATTTTTT+TGG - chr1.2:16222976-16222995 None:intergenic 15.0%
!!! TAATGAAATTTTCCATAAAT+TGG + chr1.2:16223070-16223089 MS.gene83129:intron 15.0%
!! CAAACATTGATATATTTAGA+GGG - chr1.2:16222912-16222931 None:intergenic 20.0%
!! TAGTAAAACTATGAAACATA+AGG - chr1.2:16222640-16222659 None:intergenic 20.0%
!! TATAGGGTTAAATAAGTAAA+TGG + chr1.2:16222885-16222904 MS.gene83129:intron 20.0%
!! TCAAACATTGATATATTTAG+AGG - chr1.2:16222913-16222932 None:intergenic 20.0%
!! TGTGTTTATAAATGACAATT+TGG - chr1.2:16222813-16222832 None:intergenic 20.0%
!!! ACATAATTTTCTACCATATA+TGG - chr1.2:16223003-16223022 None:intergenic 20.0%
!!! AGCTTAAATAATATTTTAGG+GGG - chr1.2:16222943-16222962 None:intergenic 20.0%
!!! ATCATATATAGCATTTTTGA+TGG + chr1.2:16223375-16223394 MS.gene83129:CDS 20.0%
!!! CATAATTTTCTACCAATTTA+TGG - chr1.2:16223085-16223104 None:intergenic 20.0%
!!! TAAATATGTTTTGTTTACGT+TGG + chr1.2:16223222-16223241 MS.gene83129:intron 20.0%
!!! TTATTTATTTTCCCTAATCA+TGG + chr1.2:16223259-16223278 MS.gene83129:intron 20.0%
! GAATTTACAGTAGGTAATTA+AGG + chr1.2:16222613-16222632 MS.gene83129:intron 25.0%
! TCATGAAATTAGTCCATATA+TGG + chr1.2:16222987-16223006 MS.gene83129:intron 25.0%
!! ACTATTTTATTGCTCATGAA+AGG + chr1.2:16222656-16222675 MS.gene83129:intron 25.0%
!!! ATGAATGAATTTTTTTGGAG+GGG - chr1.2:16222971-16222990 None:intergenic 25.0%
!!! CATGAATGAATTTTTTTGGA+GGG - chr1.2:16222972-16222991 None:intergenic 25.0%
!!! TCATGAATGAATTTTTTTGG+AGG - chr1.2:16222973-16222992 None:intergenic 25.0%
AAAATCAGAGTTCCATGATT+AGG - chr1.2:16223274-16223293 None:intergenic 30.0%
AAATCAGAGTTCCATGATTA+GGG - chr1.2:16223273-16223292 None:intergenic 30.0%
ATACGAATATGAGAAGCTAA+AGG + chr1.2:16223482-16223501 MS.gene83129:CDS 30.0%
ATATTATAGAACTTAGCACC+AGG - chr1.2:16222490-16222509 None:intergenic 30.0%
CAAGTGATTCATAAAGAACA+TGG + chr1.2:16223438-16223457 MS.gene83129:CDS 30.0%
TGAGAGTTACAAGAGTTTAA+AGG + chr1.2:16223407-16223426 MS.gene83129:CDS 30.0%
! TAACATCAACAAAAGAGTGT+TGG - chr1.2:16222725-16222744 None:intergenic 30.0%
!! TATTGATTCTCATCTTGTCA+AGG + chr1.2:16223566-16223585 MS.gene83129:CDS 30.0%
!! TTTGTTTTCTTCCTTCTGTA+GGG - chr1.2:16223311-16223330 None:intergenic 30.0%
AAGCTAAAGGAGGAAATTAC+AGG + chr1.2:16223495-16223514 MS.gene83129:CDS 35.0%
ATTCATAAAGAACATGGTGG+TGG + chr1.2:16223444-16223463 MS.gene83129:CDS 35.0%
GTGATTCATAAAGAACATGG+TGG + chr1.2:16223441-16223460 MS.gene83129:CDS 35.0%
TTCATAAAGAACATGGTGGT+GGG + chr1.2:16223445-16223464 MS.gene83129:CDS 35.0%
! AGCATTTTTGATGGAGAAGT+GGG + chr1.2:16223384-16223403 MS.gene83129:CDS 35.0%
! TAGCATTTTTGATGGAGAAG+TGG + chr1.2:16223383-16223402 MS.gene83129:CDS 35.0%
!! ATATTGGTTCTATCAAGCAC+TGG + chr1.2:16222590-16222609 MS.gene83129:CDS 35.0%
!! ATCAACAAAAGAGTGTTGGA+AGG - chr1.2:16222721-16222740 None:intergenic 35.0%
!! TATTGGTTCTATCAAGCACT+GGG + chr1.2:16222591-16222610 MS.gene83129:CDS 35.0%
!! TCAACAAAAGAGTGTTGGAA+GGG - chr1.2:16222720-16222739 None:intergenic 35.0%
!!! CTTTGTTTTCTTCCTTCTGT+AGG - chr1.2:16223312-16223331 None:intergenic 35.0%
AATATCACCACAGAAGTCCA+TGG + chr1.2:16222538-16222557 MS.gene83129:CDS 40.0%
CGAATATGAGAAGCTAAAGG+AGG + chr1.2:16223485-16223504 MS.gene83129:CDS 40.0%
CGTATCTCCTGATTCATACT+TGG + chr1.2:16223518-16223537 MS.gene83129:CDS 40.0%
GAAGGTGACTGGGAAAATAT+TGG + chr1.2:16222574-16222593 MS.gene83129:CDS 40.0%
TACTTGAGAAACCCTACAGA+AGG + chr1.2:16223297-16223316 MS.gene83129:intron 40.0%
TGGACTTCTGTGGTGATATT+AGG - chr1.2:16222538-16222557 None:intergenic 40.0%
! ATACTTGGACCTTGCTGTAA+AGG + chr1.2:16223533-16223552 MS.gene83129:CDS 40.0%
! CTTTTGTGACCTTTACAGCA+AGG - chr1.2:16223545-16223564 None:intergenic 40.0%
!! AAGCACTGGGAATTTACAGT+AGG + chr1.2:16222604-16222623 MS.gene83129:CDS 40.0%
!! ATGGTGGTGGGATTGTTAAA+TGG + chr1.2:16223457-16223476 MS.gene83129:CDS 40.0%
!! TGGTGGTGGGATTGTTAAAT+GGG + chr1.2:16223458-16223477 MS.gene83129:CDS 40.0%
GAAGTAGAGATTCAAGCACC+TGG + chr1.2:16222469-16222488 MS.gene83129:CDS 45.0%
GCAAGGTCCAAGTATGAATC+AGG - chr1.2:16223528-16223547 None:intergenic 45.0%
TAGAGTGCATGAAGGTGACT+GGG + chr1.2:16222564-16222583 MS.gene83129:CDS 45.0%
!!! TTAAAAAATTCTTTTTTTTT+TGG - chr1.2:16223118-16223137 None:intergenic 5.0%
ATCACCACAGAAGTCCATGG+AGG + chr1.2:16222541-16222560 MS.gene83129:CDS 50.0%
CATGGAGGTAGAGTGCATGA+AGG + chr1.2:16222556-16222575 MS.gene83129:CDS 50.0%
CTTCATGCACTCTACCTCCA+TGG - chr1.2:16222558-16222577 None:intergenic 50.0%
GTAGAGTGCATGAAGGTGAC+TGG + chr1.2:16222563-16222582 MS.gene83129:CDS 50.0%
TCTACCTCCATGGACTTCTG+TGG - chr1.2:16222548-16222567 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 16222442 16223593 16222442 ID=MS.gene83129
chr1.2 mRNA 16222442 16223593 16222442 ID=MS.gene83129.t1;Parent=MS.gene83129
chr1.2 exon 16222442 16222625 16222442 ID=MS.gene83129.t1.exon1;Parent=MS.gene83129.t1
chr1.2 CDS 16222442 16222625 16222442 ID=cds.MS.gene83129.t1;Parent=MS.gene83129.t1
chr1.2 exon 16223316 16223593 16223316 ID=MS.gene83129.t1.exon2;Parent=MS.gene83129.t1
chr1.2 CDS 16223316 16223593 16223316 ID=cds.MS.gene83129.t1;Parent=MS.gene83129.t1
Gene Sequence

>MS.gene83129

ATGGCTTTAAGTGGGAAAGTGGAAGCTGAAGTAGAGATTCAAGCACCTGGTGCTAAGTTCTATAATATTTTCAGAAAGCAACTAGAACATCTTCCTAATATCACCACAGAAGTCCATGGAGGTAGAGTGCATGAAGGTGACTGGGAAAATATTGGTTCTATCAAGCACTGGGAATTTACAGTAGGTAATTAAGGTCCTTATGTTTCATAGTTTTACTATTTTATTGCTCATGAAAGGTAAAATACATCAATGAACGCACAAAAAGTATTAATGAACCCTTCCAACACTCTTTTGTTGATGTTAATAAACTTTACATGATTTGATTATCAGCAAAAATAATTCATTCAAAATTTTAGCTTGTATATTATCCAAATTGTCATTTATAAACACAACAATGTTGCACTAAACAATTCTTTTGCTCAATAAAAATAAAAGATTAAAAGTATAGGGTTAAATAAGTAAATGGTCCCTCTAAATATATCAATGTTTGATTTTAGTCCCCCTAAAATATTATTTAAGCTTTTGGCCCCTCCAAAAAAATTCATTCATGAAATTAGTCCATATATGGTAGAAAATTATGTTTAAACTTCATATTTTTAGTCAACTCATGTTTAAACTTCATATTTTTTAATGAAATTTTCCATAAATTGGTAGAAAATTATGACAATATTTTCCAAAAAAAAAAGAATTTTTTAACAAAACATGAATTTAATATGAATTTTTCACCTTTTTTTTTCATTTGCTTATTAAAAAAACAAGGTATTTCAAAAATAACATCATTAAATATGTTTTGTTTACGTTGGTCTCTAATTTCTATTTATTTATTTTCCCTAATCATGGAACTCTGATTTTAAATACTTGAGAAACCCTACAGAAGGAAGAAAACAAAGTGCTAAAGCAAAAATTGAGACTATAGATGATGACAATAAGATAATCATATATAGCATTTTTGATGGAGAAGTGGGTGAGAGTTACAAGAGTTTAAAGGCAATATTACAAGTGATTCATAAAGAACATGGTGGTGGGATTGTTAAATGGGCATACGAATATGAGAAGCTAAAGGAGGAAATTACAGGCGTATCTCCTGATTCATACTTGGACCTTGCTGTAAAGGTCACAAAAGATATTGATTCTCATCTTGTCAAGGAATAG

Protein sequence

>MS.gene83129.t1

MALSGKVEAEVEIQAPGAKFYNIFRKQLEHLPNITTEVHGGRVHEGDWENIGSIKHWEFTVEGRKQSAKAKIETIDDDNKIIIYSIFDGEVGESYKSLKAILQVIHKEHGGGIVKWAYEYEKLKEEITGVSPDSYLDLAVKVTKDIDSHLVKE