AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77774


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77774.t1 MTR_8g045490 92.308 156 12 0 1 156 1 156 1.59e-105 298
MS.gene77774.t1 MTR_8g045520 81.529 157 28 1 1 156 1 157 5.45e-93 266
MS.gene77774.t1 MTR_8g045400 78.344 157 32 2 1 156 1 156 3.09e-88 254
MS.gene77774.t1 MTR_8g045570 76.316 152 36 0 1 152 1 152 1.74e-86 250
MS.gene77774.t1 MTR_8g045695 74.510 153 38 1 1 153 1 152 1.02e-83 243
MS.gene77774.t1 MTR_8g045555 75.000 152 38 0 1 152 1 152 1.41e-83 242
MS.gene77774.t1 MTR_8g045300 69.872 156 46 1 1 155 1 156 1.18e-80 235
MS.gene77774.t1 MTR_8g045735 70.588 153 44 1 1 153 1 152 7.02e-80 233
MS.gene77774.t1 MTR_8g045665 70.588 153 44 1 1 153 1 152 4.93e-78 228
MS.gene77774.t1 MTR_8g045640 69.281 153 46 1 1 153 1 152 5.67e-76 223
MS.gene77774.t1 MTR_8g045560 67.974 153 48 1 1 153 1 152 1.12e-74 220
MS.gene77774.t1 MTR_1g030810 43.137 153 84 2 1 151 1 152 1.28e-40 134
MS.gene77774.t1 MTR_1g030820 40.523 153 88 2 1 151 1 152 2.93e-40 133
MS.gene77774.t1 MTR_1g030840 37.179 156 90 4 1 149 1 155 4.59e-29 104
MS.gene77774.t1 MTR_8g012550 35.616 146 91 3 6 150 8 151 1.57e-28 103
MS.gene77774.t1 MTR_1g031640 38.514 148 82 5 7 152 7 147 5.21e-26 96.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77774.t1 AT5G28010 36.242 149 92 3 3 149 15 162 7.03e-27 99.4
MS.gene77774.t1 AT5G28010 36.242 149 92 3 3 149 15 162 7.03e-27 99.4
MS.gene77774.t1 AT1G70890 37.584 149 90 3 3 149 7 154 2.70e-26 97.8
MS.gene77774.t1 AT1G70830 34.899 149 94 3 3 149 22 169 5.31e-24 92.0
MS.gene77774.t1 AT1G70830 34.899 149 94 3 3 149 22 169 9.91e-24 92.4
MS.gene77774.t1 AT1G70840 32.886 149 97 3 3 149 20 167 2.25e-23 90.5
MS.gene77774.t1 AT1G70830 34.899 149 94 3 3 149 22 169 3.49e-23 92.0
MS.gene77774.t1 AT5G28000 32.886 149 97 3 3 149 13 160 9.31e-23 88.6
MS.gene77774.t1 AT1G70830 34.899 149 94 3 3 149 22 169 9.60e-23 92.4
MS.gene77774.t1 AT1G70830 33.557 149 96 3 3 149 184 331 3.49e-21 88.2
MS.gene77774.t1 AT1G70870 35.948 153 81 4 1 151 1 138 8.43e-22 85.5
MS.gene77774.t1 AT1G70830 33.557 149 96 3 3 149 137 284 1.27e-21 88.6
MS.gene77774.t1 AT1G70830 30.435 92 63 1 59 149 31 122 6.30e-11 59.3
MS.gene77774.t1 AT1G70850 32.886 149 97 3 3 149 9 156 1.61e-21 87.4
MS.gene77774.t1 AT1G23120 30.769 143 94 3 7 149 7 144 4.28e-21 84.0
MS.gene77774.t1 AT1G70850 32.886 149 97 3 3 149 9 156 4.56e-21 87.4
MS.gene77774.t1 AT1G70850 33.094 139 90 3 13 149 175 312 8.77e-20 84.0
MS.gene77774.t1 AT1G70850 32.886 149 97 3 3 149 9 156 4.56e-21 87.4
MS.gene77774.t1 AT1G70850 33.094 139 90 3 13 149 175 312 8.77e-20 84.0
MS.gene77774.t1 AT4G23670 34.641 153 97 3 1 152 1 151 7.32e-21 83.6
MS.gene77774.t1 AT1G23130 30.405 148 101 2 3 149 10 156 1.18e-20 83.2
MS.gene77774.t1 AT1G23120 30.769 143 94 3 7 149 57 194 1.26e-20 84.0
MS.gene77774.t1 AT1G70880 30.872 149 100 3 3 149 8 155 5.15e-20 81.6
MS.gene77774.t1 AT2G01520 32.026 153 101 3 1 152 1 151 7.20e-19 78.2
MS.gene77774.t1 AT4G23680 32.026 153 101 3 1 152 1 151 2.83e-18 76.6
MS.gene77774.t1 AT1G14930 32.298 161 92 5 1 153 1 152 1.12e-17 75.5
MS.gene77774.t1 AT2G01530 32.026 153 101 3 1 152 1 151 2.20e-17 74.3
MS.gene77774.t1 AT4G14060 32.680 153 100 3 1 152 1 151 2.78e-17 74.3
MS.gene77774.t1 AT1G35260 28.966 145 99 3 7 149 6 148 3.64e-17 73.9
MS.gene77774.t1 AT1G24020 32.692 156 103 2 1 155 1 155 4.95e-16 71.2
MS.gene77774.t1 AT1G24020 32.692 156 103 2 1 155 1 155 4.95e-16 71.2
MS.gene77774.t1 AT1G30990 32.000 150 98 4 1 149 1 147 2.12e-15 69.3
MS.gene77774.t1 AT1G14940 30.000 150 102 3 1 149 1 148 3.65e-14 66.2
MS.gene77774.t1 AT3G26460 32.051 156 98 5 1 152 1 152 8.17e-14 65.1
MS.gene77774.t1 AT1G14950 28.947 152 105 3 1 151 1 150 1.34e-13 64.7
MS.gene77774.t1 AT1G35310 25.362 138 100 3 13 149 12 147 1.95e-12 61.6
MS.gene77774.t1 AT1G14960 28.571 154 107 3 1 153 1 152 8.27e-12 60.1
MS.gene77774.t1 AT3G26450 27.273 154 108 4 1 152 1 152 2.88e-11 58.5

Find 27 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8.1:+59984033 MS.gene77774:CDS
CACCCTTATCAATGACTTCA+AGG 0.380806 8.1:-59984010 None:intergenic
TTATAGAGCTATCTTTGCTT+TGG 0.392094 8.1:-59984153 None:intergenic
AAACACTGGACTTACGTCAT+AGG 0.436108 8.1:+59983093 MS.gene77774:CDS
TTCTAAGAAATGGTGCTCTC+TGG 0.436928 8.1:+59982919 None:intergenic
CATCCTTGAAGTCATTGATA+AGG 0.445764 8.1:+59984007 MS.gene77774:CDS
AAGTATTCCATGTAGCCATT+TGG 0.448595 8.1:-59984095 None:intergenic
AACACTTATAAGCTCTTTGG+TGG 0.457999 8.1:+59983951 MS.gene77774:CDS
AACTTCATGAAGGTGAAGAT+TGG 0.478377 8.1:+59983052 MS.gene77774:CDS
GGTAAGCTCAGTACTGAAGT+TGG 0.490802 8.1:+59982940 MS.gene77774:CDS
ATCCTTGAAGTCATTGATAA+GGG 0.494628 8.1:+59984008 MS.gene77774:CDS
AGATATTGATGAGCACTATA+AGG 0.500590 8.1:+59983974 MS.gene77774:CDS
GAACAGATTGTAGAACTTGT+CGG 0.501167 8.1:-59982977 None:intergenic
TATTCCATGTAGCCATTTGG+AGG 0.517921 8.1:-59984092 None:intergenic
AGATTGGCATCACACTGATT+CGG 0.519723 8.1:+59983068 MS.gene77774:CDS
AATGGCTACATGGAATACTT+TGG 0.525683 8.1:+59984098 MS.gene77774:CDS
TGACCCTCCAAATGGCTACA+TGG 0.526835 8.1:+59984088 MS.gene77774:CDS
GAGGATATTGACCCTCCAAA+TGG 0.540200 8.1:+59984080 MS.gene77774:CDS
TATGATGAACTCTTTCACAA+AGG 0.552779 8.1:-59983026 None:intergenic
GAAGTCATTGATAAGGGTGA+TGG 0.579318 8.1:+59984014 MS.gene77774:CDS
ATCATAATTCACAGATGGCA+AGG 0.582682 8.1:+59983878 MS.gene77774:intron
CATCATACAAAACTTCATGA+AGG 0.606616 8.1:+59983042 MS.gene77774:CDS
ACACTGATTCGGTTAAACAC+TGG 0.614381 8.1:+59983079 MS.gene77774:CDS
ATTCCATGTAGCCATTTGGA+GGG 0.625101 8.1:-59984091 None:intergenic
AGATTGTAGAACTTGTCGGC+GGG 0.646397 8.1:-59982973 None:intergenic
TGAATATGAAAAGATCAATG+AGG 0.667026 8.1:+59984061 MS.gene77774:CDS
CAGATTGTAGAACTTGTCGG+CGG 0.688816 8.1:-59982974 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGAATAATAAATATTTATA+AGG - chr8.1:59983724-59983743 None:intergenic 10.0%
!!! ATAGTTTATTTGTTAAAAAA+TGG + chr8.1:59983787-59983806 MS.gene77774:intron 10.0%
!!! TAGTTTATTTGTTAAAAAAT+GGG + chr8.1:59983788-59983807 MS.gene77774:intron 10.0%
!! AAAAAAAATCAACAATCATT+TGG + chr8.1:59983481-59983500 MS.gene77774:intron 15.0%
!! AAAAAAATCAACAATCATTT+GGG + chr8.1:59983482-59983501 MS.gene77774:intron 15.0%
!! AATTAAATAAGATGATGATT+TGG - chr8.1:59983155-59983174 None:intergenic 15.0%
!! ACAAATAAAAATGAAAACTT+TGG - chr8.1:59983842-59983861 None:intergenic 15.0%
!!! GTTGATTTTTTTTTGTCATA+AGG - chr8.1:59983474-59983493 None:intergenic 20.0%
! AAAAACACTTATAAGCTCTT+TGG + chr8.1:59983948-59983967 MS.gene77774:CDS 25.0%
! AACCACAGTTATTCTTTATA+TGG - chr8.1:59983699-59983718 None:intergenic 25.0%
! AGATTATGAGATCTCAAAAA+TGG + chr8.1:59983578-59983597 MS.gene77774:intron 25.0%
! ATAAGAAAGAAACAAGTACA+TGG + chr8.1:59983261-59983280 MS.gene77774:intron 25.0%
! TGAATATGAAAAGATCAATG+AGG + chr8.1:59984061-59984080 MS.gene77774:CDS 25.0%
! TTGGGAAGAAAAAAAAATCA+GGG - chr8.1:59983344-59983363 None:intergenic 25.0%
! TTTGGGAAGAAAAAAAAATC+AGG - chr8.1:59983345-59983364 None:intergenic 25.0%
!! CTATCTTGAAAATGGTTTTT+GGG - chr8.1:59983362-59983381 None:intergenic 25.0%
!! TCTATCTTGAAAATGGTTTT+TGG - chr8.1:59983363-59983382 None:intergenic 25.0%
AACATAGTCAAAAGTAGAAG+TGG - chr8.1:59983405-59983424 None:intergenic 30.0%
AGATATTGATGAGCACTATA+AGG + chr8.1:59983974-59983993 MS.gene77774:CDS 30.0%
AGCCATATAAAGAATAACTG+TGG + chr8.1:59983694-59983713 MS.gene77774:intron 30.0%
ATCCTTGAAGTCATTGATAA+GGG + chr8.1:59984008-59984027 MS.gene77774:CDS 30.0%
CATCATACAAAACTTCATGA+AGG + chr8.1:59983042-59983061 MS.gene77774:CDS 30.0%
TACATTCTACAAACCAAACA+TGG + chr8.1:59983314-59983333 MS.gene77774:intron 30.0%
TATGATGAACTCTTTCACAA+AGG - chr8.1:59983029-59983048 None:intergenic 30.0%
TGGGAAGAAAAAAAAATCAG+GGG - chr8.1:59983343-59983362 None:intergenic 30.0%
TTATAGAGCTATCTTTGCTT+TGG - chr8.1:59984156-59984175 None:intergenic 30.0%
! TGAAGCATCTATCTTGAAAA+TGG - chr8.1:59983370-59983389 None:intergenic 30.0%
! TTTTTGTCATAAGGTTCTCT+GGG - chr8.1:59983465-59983484 None:intergenic 30.0%
!! CTACTTTTGACTATGTTGAA+CGG + chr8.1:59983408-59983427 MS.gene77774:intron 30.0%
!! TACTTTTGACTATGTTGAAC+GGG + chr8.1:59983409-59983428 MS.gene77774:intron 30.0%
!!! TTTTTTGTCATAAGGTTCTC+TGG - chr8.1:59983466-59983485 None:intergenic 30.0%
AACACTTATAAGCTCTTTGG+TGG + chr8.1:59983951-59983970 MS.gene77774:CDS 35.0%
AACTTCATGAAGGTGAAGAT+TGG + chr8.1:59983052-59983071 MS.gene77774:CDS 35.0%
AAGTATTCCATGTAGCCATT+TGG - chr8.1:59984098-59984117 None:intergenic 35.0%
AATGGCTACATGGAATACTT+TGG + chr8.1:59984098-59984117 MS.gene77774:CDS 35.0%
ATAGTCAAAAGTAGAAGTGG+TGG - chr8.1:59983402-59983421 None:intergenic 35.0%
ATCATAATTCACAGATGGCA+AGG + chr8.1:59983878-59983897 MS.gene77774:intron 35.0%
ATCTGTGAATTATGATGGCA+CGG - chr8.1:59983874-59983893 None:intergenic 35.0%
CATCCTTGAAGTCATTGATA+AGG + chr8.1:59984007-59984026 MS.gene77774:CDS 35.0%
GAACAGATTGTAGAACTTGT+CGG - chr8.1:59982980-59982999 None:intergenic 35.0%
TTGCCATCTGTGAATTATGA+TGG - chr8.1:59983879-59983898 None:intergenic 35.0%
! TTTTGTCATAAGGTTCTCTG+GGG - chr8.1:59983464-59983483 None:intergenic 35.0%
!! TGATGATTTGGTTATCGATC+AGG - chr8.1:59983143-59983162 None:intergenic 35.0%
AAACACTGGACTTACGTCAT+AGG + chr8.1:59983093-59983112 MS.gene77774:CDS 40.0%
AAATCAGGGGTAACCATGTT+TGG - chr8.1:59983330-59983349 None:intergenic 40.0%
ATTCCATGTAGCCATTTGGA+GGG - chr8.1:59984094-59984113 None:intergenic 40.0%
CACCCTTATCAATGACTTCA+AGG - chr8.1:59984013-59984032 None:intergenic 40.0%
GAAGTCATTGATAAGGGTGA+TGG + chr8.1:59984014-59984033 MS.gene77774:CDS 40.0%
GTGCCATCATAATTCACAGA+TGG + chr8.1:59983873-59983892 MS.gene77774:intron 40.0%
TATTCCATGTAGCCATTTGG+AGG - chr8.1:59984095-59984114 None:intergenic 40.0%
TCTGTGAATTATGATGGCAC+GGG - chr8.1:59983873-59983892 None:intergenic 40.0%
! AGATTGGCATCACACTGATT+CGG + chr8.1:59983068-59983087 MS.gene77774:CDS 40.0%
! ATAAGGTTCTCTGGGGAATA+TGG - chr8.1:59983457-59983476 None:intergenic 40.0%
!! ACACTGATTCGGTTAAACAC+TGG + chr8.1:59983079-59983098 MS.gene77774:CDS 40.0%
AGATTGTAGAACTTGTCGGC+GGG - chr8.1:59982976-59982995 None:intergenic 45.0%
CAGATTGTAGAACTTGTCGG+CGG - chr8.1:59982977-59982996 None:intergenic 45.0%
GAGGATATTGACCCTCCAAA+TGG + chr8.1:59984080-59984099 MS.gene77774:CDS 45.0%
! GGTAAGCTCAGTACTGAAGT+TGG + chr8.1:59982940-59982959 MS.gene77774:CDS 45.0%
!!! AATATTTTTGTATAAAAAAA+AGG - chr8.1:59983744-59983763 None:intergenic 5.0%
!!! TTTTATACAAAAATATTTAT+TGG + chr8.1:59983747-59983766 MS.gene77774:intron 5.0%
ATGGCACTGCTGCTGCTAAA+TGG + chr8.1:59984033-59984052 MS.gene77774:CDS 50.0%
TGACCCTCCAAATGGCTACA+TGG + chr8.1:59984088-59984107 MS.gene77774:CDS 50.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 59982928 59984175 59982928 ID=MS.gene77774
chr8.1 mRNA 59982928 59984175 59982928 ID=MS.gene77774.t1;Parent=MS.gene77774
chr8.1 exon 59982928 59983114 59982928 ID=MS.gene77774.t1.exon1;Parent=MS.gene77774.t1
chr8.1 CDS 59982928 59983114 59982928 ID=cds.MS.gene77774.t1;Parent=MS.gene77774.t1
chr8.1 exon 59983892 59984175 59983892 ID=MS.gene77774.t1.exon2;Parent=MS.gene77774.t1
chr8.1 CDS 59983892 59984175 59983892 ID=cds.MS.gene77774.t1;Parent=MS.gene77774.t1
Gene Sequence

>MS.gene77774

ATGGTGCTCTCTGGTAAGCTCAGTACTGAAGTTGGAATCAAAACACCCGCCGACAAGTTCTACAATCTGTTCATAACAGAACTTCATGAAGTGCAAAACCTTTGTGAAAGAGTTCATCATACAAAACTTCATGAAGGTGAAGATTGGCATCACACTGATTCGGTTAAACACTGGACTTACGTCATAGGTAATTATGTTTCTAATTCAGTTCACCTGATCGATAACCAAATCATCATCTTATTTAATTTGAAATTTTTTTTATGTTTTATTTTTATTCTTAATGATAACTATACTTCTAATAACAAAAAAAAAAAATTGTGAATTAAAAGAAATATAAGAAAGAAACAAGTACATGGTTACTTATCAATACATGAATTACTTATCAATACATTCTACAAACCAAACATGGTTACCCCTGATTTTTTTTTCTTCCCAAAAACCATTTTCAAGATAGATGCTTCATCATTTCAACCACCACTTCTACTTTTGACTATGTTGAACGGGAAATATAATCATTCATAAGCTTCCATATTCCCCAGAGAACCTTATGACAAAAAAAAATCAACAATCATTTGGGTAACTCATCTCTTAAAGTTTTAAATACAATGTTAACAAATGTGTTCAATTTAATTTGTAAATTTTGTTAATATAGATTATGAGATCTCAAAAATGGTTAAATGAATAAAAAGTAATGAAAAGAAGTATATAAAAATCATGAATTATGTAAAAAAAAAAACAATTATGAATTATTTGAGAGATTAAAATCAGCCATATAAAGAATAACTGTGGTTTACCTTATAAATATTTATTATTCCTTTTTTTTATACAAAAATATTTATTGGTAAAATAAAACAATATAATAGTTTATTTGTTAAAAAATGGGTGATTGTATTTTTCTGTAGTTGCTATATCCAAAGTTTTCATTTTTATTTGTATTAATTTCCCGTGCCATCATAATTCACAGATGGCAAGGTACATACATGTCACGAGAGAGTTGAAGAAGTTGATGAACTGAATAAAAAAAACACTTATAAGCTCTTTGGTGGAGATATTGATGAGCACTATAAGGTCTTTAAGCTCATCCTTGAAGTCATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAAAAGATCAATGAGGATATTGACCCTCCAAATGGCTACATGGAATACTTTGGCAAAATCACTAGAGATGTTGATGCTCATCTTGCCAAAGCAAAGATAGCTCTATAA

Protein sequence

>MS.gene77774.t1

MVLSGKLSTEVGIKTPADKFYNLFITELHEVQNLCERVHHTKLHEGEDWHHTDSVKHWTYVIDGKVHTCHERVEEVDELNKKNTYKLFGGDIDEHYKVFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGYMEYFGKITRDVDAHLAKAKIAL