AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59863


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59863.t1 MTR_8g045695 96.154 156 6 0 1 156 1 156 6.75e-111 311
MS.gene59863.t1 MTR_8g045735 88.312 154 18 0 1 154 1 154 1.42e-101 288
MS.gene59863.t1 MTR_8g045665 87.179 156 20 0 1 156 1 156 7.03e-100 284
MS.gene59863.t1 MTR_8g045640 83.333 156 26 0 1 156 1 156 6.99e-96 274
MS.gene59863.t1 MTR_8g045560 82.692 156 27 0 1 156 1 156 1.32e-94 270
MS.gene59863.t1 MTR_8g045520 78.981 157 32 1 1 156 1 157 1.48e-89 258
MS.gene59863.t1 MTR_8g045490 75.159 157 37 2 1 156 1 156 9.45e-86 248
MS.gene59863.t1 MTR_8g045400 73.248 157 40 2 1 156 1 156 3.48e-83 241
MS.gene59863.t1 MTR_8g045570 71.711 152 42 1 1 151 1 152 9.41e-81 235
MS.gene59863.t1 MTR_8g045555 71.053 152 43 1 1 151 1 152 7.08e-80 233
MS.gene59863.t1 MTR_8g045300 67.949 156 49 1 1 155 1 156 6.56e-79 231
MS.gene59863.t1 MTR_1g030810 43.137 153 83 3 1 150 1 152 1.54e-39 131
MS.gene59863.t1 MTR_1g030820 38.562 153 90 3 1 150 1 152 1.21e-37 126
MS.gene59863.t1 MTR_1g030840 38.462 156 87 5 1 148 1 155 6.28e-29 104
MS.gene59863.t1 MTR_8g012550 34.000 150 95 4 6 153 8 155 2.32e-26 97.8
MS.gene59863.t1 MTR_1g031640 38.514 148 81 6 7 151 7 147 1.88e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59863.t1 AT5G28010 35.948 153 94 4 3 152 15 166 1.27e-25 96.3
MS.gene59863.t1 AT5G28010 35.948 153 94 4 3 152 15 166 1.27e-25 96.3
MS.gene59863.t1 AT1G70840 35.294 153 95 4 3 152 20 171 9.76e-25 94.0
MS.gene59863.t1 AT1G70830 36.601 153 93 4 3 152 22 173 2.61e-24 92.8
MS.gene59863.t1 AT1G70830 36.364 154 94 4 3 153 22 174 3.83e-24 93.2
MS.gene59863.t1 AT1G70890 35.948 153 94 4 3 152 7 158 6.07e-24 91.7
MS.gene59863.t1 AT1G70830 36.364 154 94 4 3 153 22 174 1.70e-23 92.8
MS.gene59863.t1 AT1G70830 36.364 154 94 4 3 153 22 174 4.34e-23 93.2
MS.gene59863.t1 AT1G70830 35.294 153 95 4 3 152 184 335 1.52e-21 89.0
MS.gene59863.t1 AT5G28000 32.680 153 99 4 3 152 13 164 2.82e-22 87.4
MS.gene59863.t1 AT1G70830 35.294 153 95 4 3 152 137 288 5.28e-22 89.7
MS.gene59863.t1 AT1G70830 32.990 97 63 2 59 153 31 127 3.56e-11 60.1
MS.gene59863.t1 AT1G70850 34.667 150 94 4 3 149 9 157 1.04e-21 87.8
MS.gene59863.t1 AT1G70850 34.667 150 94 4 3 149 9 157 2.33e-21 88.2
MS.gene59863.t1 AT1G70850 35.664 143 88 4 13 152 175 316 7.32e-21 87.0
MS.gene59863.t1 AT1G70850 34.667 150 94 4 3 149 9 157 2.33e-21 88.2
MS.gene59863.t1 AT1G70850 35.664 143 88 4 13 152 175 316 7.32e-21 87.0
MS.gene59863.t1 AT4G23670 32.680 153 99 4 1 151 1 151 1.99e-20 82.4
MS.gene59863.t1 AT1G14930 29.299 157 107 4 1 155 1 155 2.63e-19 79.7
MS.gene59863.t1 AT1G70870 34.839 155 79 5 1 150 1 138 6.98e-19 78.2
MS.gene59863.t1 AT2G01520 29.412 153 104 4 1 151 1 151 1.07e-18 77.8
MS.gene59863.t1 AT2G01530 30.719 153 102 4 1 151 1 151 9.47e-18 75.5
MS.gene59863.t1 AT1G70880 29.412 153 104 4 3 152 8 159 3.86e-17 73.9
MS.gene59863.t1 AT1G14940 28.662 157 108 4 1 155 1 155 5.89e-17 73.6
MS.gene59863.t1 AT1G35260 30.612 147 91 5 9 149 8 149 2.25e-16 72.0
MS.gene59863.t1 AT4G14060 28.758 153 105 4 1 151 1 151 2.29e-16 72.0
MS.gene59863.t1 AT4G23680 28.947 152 104 4 1 150 1 150 2.93e-16 71.6
MS.gene59863.t1 AT1G23130 28.188 149 104 3 3 149 10 157 4.69e-16 71.2
MS.gene59863.t1 AT1G24020 30.968 155 98 4 1 150 1 151 4.89e-16 71.2
MS.gene59863.t1 AT1G24020 30.968 155 98 4 1 150 1 151 4.89e-16 71.2
MS.gene59863.t1 AT1G14950 28.025 157 109 4 1 155 1 155 7.89e-16 70.5
MS.gene59863.t1 AT1G23120 28.667 150 99 5 3 149 1 145 1.71e-15 69.3
MS.gene59863.t1 AT1G23120 27.660 141 96 4 10 149 60 195 4.49e-15 69.7
MS.gene59863.t1 AT1G30990 27.815 151 104 5 1 149 1 148 1.52e-13 64.3
MS.gene59863.t1 AT1G14960 26.452 155 110 4 1 153 1 153 8.47e-13 62.4
MS.gene59863.t1 AT1G35310 27.338 139 97 4 13 149 12 148 1.56e-12 62.0
MS.gene59863.t1 AT3G26460 26.797 153 109 3 1 151 1 152 2.21e-12 61.6
MS.gene59863.t1 AT3G26450 24.675 154 111 5 1 151 1 152 4.16e-11 58.2

Find 28 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGGAAATTCTGCTGTTAGA+TGG 0.411619 8.3:+53668768 MS.gene59863:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8.3:+53667476 MS.gene59863:CDS
AAAATCACATACAAGCTGTT+TGG 0.429054 8.3:+53668686 MS.gene59863:CDS
TGGAGATATTGAAAACTATA+AGG 0.439418 8.3:+53668709 MS.gene59863:CDS
AAACACTGGACTTATGTCAT+AGG 0.470664 8.3:+53667629 MS.gene59863:CDS
AGGTAGTCCATCCATCCATT+TGG 0.478602 8.3:-53668830 None:intergenic
AGCTTCATGAGGGTGAAGAT+TGG 0.493681 8.3:+53667588 MS.gene59863:CDS
TCATCTTGTCAAAGGGGAAA+AGG 0.504175 8.3:+53668880 MS.gene59863:CDS
AGATTGGCATCATACTGATT+CGG 0.504369 8.3:+53667604 MS.gene59863:CDS
AAGAGCACTAGAGATATTGA+TGG 0.505384 8.3:+53668857 MS.gene59863:CDS
TTGATGGTCATCTTGTCAAA+GGG 0.506740 8.3:+53668873 MS.gene59863:CDS
ATCACATACAAGCTGTTTGG+TGG 0.517987 8.3:+53668689 MS.gene59863:CDS
ATCTTCACCCTCATGAAGCT+TGG 0.519812 8.3:-53667585 None:intergenic
GTAAACTTATTACTGAACTT+GGG 0.542207 8.3:+53667477 MS.gene59863:CDS
ATTGATGGTCATCTTGTCAA+AGG 0.543582 8.3:+53668872 MS.gene59863:CDS
GAGGATGTTGATACTCCAAA+TGG 0.546873 8.3:+53668815 MS.gene59863:CDS
AGTTTGTAGAACTTGTCAGC+TGG 0.569465 8.3:-53667509 None:intergenic
TATCTCTAGTGCTCTTGCTA+AGG 0.569595 8.3:-53668850 None:intergenic
GAAGTGCTTAACAAGGCTGA+TGG 0.573383 8.3:+53668749 MS.gene59863:CDS
TGATACTCCAAATGGATGGA+TGG 0.602025 8.3:+53668823 MS.gene59863:CDS
ATACTGATTCGGTTAAACAC+TGG 0.606046 8.3:+53667615 MS.gene59863:CDS
TCATCATACCAAGCTTCATG+AGG 0.610699 8.3:+53667577 MS.gene59863:CDS
ATGTTGATACTCCAAATGGA+TGG 0.627228 8.3:+53668819 MS.gene59863:CDS
CATCATACCAAGCTTCATGA+GGG 0.642119 8.3:+53667578 MS.gene59863:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8.3:+53668742 MS.gene59863:CDS
TGATGGTCATCTTGTCAAAG+GGG 0.652198 8.3:+53668874 MS.gene59863:CDS
TGACATAACAACAGATGGCG+AGG 0.663434 8.3:+53668616 MS.gene59863:intron
TGATTATGAGAAGAACAATG+AGG 0.694185 8.3:+53668796 MS.gene59863:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACTATAAAATAGTA+AGG + chr8.3:53668386-53668405 MS.gene59863:intron 10.0%
!! TTATCTTATTATAATATTTC+TGG + chr8.3:53668524-53668543 MS.gene59863:intron 10.0%
!!! AAAACTTATTTTATTCTTTT+TGG + chr8.3:53668580-53668599 MS.gene59863:intron 10.0%
!!! TTATTTTACTAAAAACTTTA+AGG - chr8.3:53668291-53668310 None:intergenic 10.0%
!! AGATAATCTATATAAAAAGT+AGG + chr8.3:53668224-53668243 MS.gene59863:intron 15.0%
!! GATAATCTATATAAAAAGTA+GGG + chr8.3:53668225-53668244 MS.gene59863:intron 15.0%
!! TATAGTAATAAAAAGAAGAT+TGG + chr8.3:53667739-53667758 MS.gene59863:intron 15.0%
!!! GAATAAAATAAGTTTTTCAA+AGG - chr8.3:53668576-53668595 None:intergenic 15.0%
!! CATCATATAAGATCTTTAAA+AGG + chr8.3:53668427-53668446 MS.gene59863:intron 20.0%
! AAAAAATTATACTCGTACCA+TGG - chr8.3:53668165-53668184 None:intergenic 25.0%
! AGTAATAAAAAGAAGATTGG+TGG + chr8.3:53667742-53667761 MS.gene59863:intron 25.0%
! GTAAACTTATTACTGAACTT+GGG + chr8.3:53667477-53667496 MS.gene59863:CDS 25.0%
! GTAATAAAAAGAAGATTGGT+GGG + chr8.3:53667743-53667762 MS.gene59863:intron 25.0%
! TATCAAACAAGATGATGATT+TGG - chr8.3:53667681-53667700 None:intergenic 25.0%
! TCTTCGTTATAGAATTCATT+TGG - chr8.3:53667779-53667798 None:intergenic 25.0%
! TGGAGATATTGAAAACTATA+AGG + chr8.3:53668709-53668728 MS.gene59863:CDS 25.0%
! TTATCTCTAGACAAAAAATC+TGG - chr8.3:53668210-53668229 None:intergenic 25.0%
!!! ATCTTTAAAAGGTGTTTTAG+AGG + chr8.3:53668438-53668457 MS.gene59863:intron 25.0%
AAAATCACATACAAGCTGTT+TGG + chr8.3:53668686-53668705 MS.gene59863:CDS 30.0%
GGTAAACTTATTACTGAACT+TGG + chr8.3:53667476-53667495 MS.gene59863:CDS 30.0%
GTTTGTATGATATGATCCTT+CGG + chr8.3:53668247-53668266 MS.gene59863:intron 30.0%
TATCGAAATCATTGTACACA+AGG + chr8.3:53667957-53667976 MS.gene59863:intron 30.0%
TGATTATGAGAAGAACAATG+AGG + chr8.3:53668796-53668815 MS.gene59863:CDS 30.0%
AAACACTGGACTTATGTCAT+AGG + chr8.3:53667629-53667648 MS.gene59863:CDS 35.0%
AAAGCAACAAATGACCCTAA+AGG + chr8.3:53668107-53668126 MS.gene59863:intron 35.0%
AGTAGCATCTCTTTCCTTTA+GGG - chr8.3:53668124-53668143 None:intergenic 35.0%
ATGGAAATTCTGCTGTTAGA+TGG + chr8.3:53668768-53668787 MS.gene59863:CDS 35.0%
ATGTTGATACTCCAAATGGA+TGG + chr8.3:53668819-53668838 MS.gene59863:CDS 35.0%
! AGATTGGCATCATACTGATT+CGG + chr8.3:53667604-53667623 MS.gene59863:CDS 35.0%
! ATTGATGGTCATCTTGTCAA+AGG + chr8.3:53668872-53668891 MS.gene59863:CDS 35.0%
! TTGATGGTCATCTTGTCAAA+GGG + chr8.3:53668873-53668892 MS.gene59863:CDS 35.0%
!! AAGAGCACTAGAGATATTGA+TGG + chr8.3:53668857-53668876 MS.gene59863:CDS 35.0%
!! ATACTGATTCGGTTAAACAC+TGG + chr8.3:53667615-53667634 MS.gene59863:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG + chr8.3:53668742-53668761 MS.gene59863:CDS 35.0%
AACCACCTTTGTATTCACCA+TGG + chr8.3:53668145-53668164 MS.gene59863:intron 40.0%
AGTTTGTAGAACTTGTCAGC+TGG - chr8.3:53667512-53667531 None:intergenic 40.0%
ATCACATACAAGCTGTTTGG+TGG + chr8.3:53668689-53668708 MS.gene59863:CDS 40.0%
CATCATACCAAGCTTCATGA+GGG + chr8.3:53667578-53667597 MS.gene59863:CDS 40.0%
GAGGATGTTGATACTCCAAA+TGG + chr8.3:53668815-53668834 MS.gene59863:CDS 40.0%
GAGTAGCATCTCTTTCCTTT+AGG - chr8.3:53668125-53668144 None:intergenic 40.0%
TACCATGGTGAATACAAAGG+TGG - chr8.3:53668150-53668169 None:intergenic 40.0%
TATCTCTAGTGCTCTTGCTA+AGG - chr8.3:53668853-53668872 None:intergenic 40.0%
TCATCATACCAAGCTTCATG+AGG + chr8.3:53667577-53667596 MS.gene59863:CDS 40.0%
TCATCTTGTCAAAGGGGAAA+AGG + chr8.3:53668880-53668899 MS.gene59863:CDS 40.0%
TCGTACCATGGTGAATACAA+AGG - chr8.3:53668153-53668172 None:intergenic 40.0%
TGAGACTGATCTAACAACGA+AGG + chr8.3:53667816-53667835 MS.gene59863:intron 40.0%
TGATACTCCAAATGGATGGA+TGG + chr8.3:53668823-53668842 MS.gene59863:CDS 40.0%
TGATGGTCATCTTGTCAAAG+GGG + chr8.3:53668874-53668893 MS.gene59863:CDS 40.0%
TTGTACACAAGGTGAAGTAC+TGG + chr8.3:53667968-53667987 MS.gene59863:intron 40.0%
! CGTCTTTAGACATTTTCCGA+AGG - chr8.3:53668266-53668285 None:intergenic 40.0%
AGCTTCATGAGGGTGAAGAT+TGG + chr8.3:53667588-53667607 MS.gene59863:CDS 45.0%
AGGTAGTCCATCCATCCATT+TGG - chr8.3:53668833-53668852 None:intergenic 45.0%
ATCTTCACCCTCATGAAGCT+TGG - chr8.3:53667588-53667607 None:intergenic 45.0%
GCCATCTGTTGTTATGTCAG+AGG - chr8.3:53668615-53668634 None:intergenic 45.0%
GCCTCTGACATAACAACAGA+TGG + chr8.3:53668611-53668630 MS.gene59863:intron 45.0%
TGACATAACAACAGATGGCG+AGG + chr8.3:53668616-53668635 MS.gene59863:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG + chr8.3:53668749-53668768 MS.gene59863:CDS 45.0%
!! AAATAATATATATTATGAAT+GGG - chr8.3:53668062-53668081 None:intergenic 5.0%
!! AATATATATTATTTAGAAAA+AGG + chr8.3:53668068-53668087 MS.gene59863:intron 5.0%
!! AATTAATAAAAAAAAAGTAA+CGG - chr8.3:53668022-53668041 None:intergenic 5.0%
!! TAAATAATATATATTATGAA+TGG - chr8.3:53668063-53668082 None:intergenic 5.0%
!!! TAATTTAGAATTTTTTTAAT+TGG + chr8.3:53667860-53667879 MS.gene59863:intron 5.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 53667464 53668913 53667464 ID=MS.gene59863
chr8.3 mRNA 53667464 53668913 53667464 ID=MS.gene59863.t1;Parent=MS.gene59863
chr8.3 exon 53667464 53667650 53667464 ID=MS.gene59863.t1.exon1;Parent=MS.gene59863.t1
chr8.3 CDS 53667464 53667650 53667464 ID=cds.MS.gene59863.t1;Parent=MS.gene59863.t1
chr8.3 exon 53668630 53668913 53668630 ID=MS.gene59863.t1.exon2;Parent=MS.gene59863.t1
chr8.3 CDS 53668630 53668913 53668630 ID=cds.MS.gene59863.t1;Parent=MS.gene59863.t1
Gene Sequence

>MS.gene59863

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATAAAAACACCAGCTGACAAGTTCTACAAACTATTTGCATCAGAACTTCACGAAGTGCAAAATGTTTGTGAAAGAGTTCATCATACCAAGCTTCATGAGGGTGAAGATTGGCATCATACTGATTCGGTTAAACACTGGACTTATGTCATAGGTAATTCTATTTCTAATGTTTCGATAACCAAATCATCATCTTGTTTGATATGAAAAAGTTTTCTATTACAAATTTTTTTAATGATAATTATAGTAATAAAAAGAAGATTGGTGGGTTAAAGAATACCAAATGAATTCTATAACGAAGAATAAAGATTTGATTCATTGAGACTGATCTAACAACGAAGGCAATATTTTAGACTATTGACTTAATTTAGAATTTTTTTAATTGGTTAGAAAGTTTGTAAGAGTTTTAGATATATAAATTGTTAAAATAAAAAGTTAAATTCATGAAAAAAAATTGAGATATCGAAATCATTGTACACAAGGTGAAGTACTGGATCTCTAAAAATAGTACTTAAACTTTTTCCGTTACTTTTTTTTTATTAATTAAGAATGAGCTTGAACACCCATTCATAATATATATTATTTAGAAAAAGGAAGAGAAAAAAAATAAAAAGCAACAAATGACCCTAAAGGAAAGAGATGCTACTCAACCACCTTTGTATTCACCATGGTACGAGTATAATTTTTTCTTTACGAATGTAATTACATTTCCAGATTTTTTGTCTAGAGATAATCTATATAAAAAGTAGGGTTTGTATGATATGATCCTTCGGAAAATGTCTAAAGACGAACCTTAAAGTTTTTAGTAAAATAATGTATTTTATCATAATATTTTAAAAACAGATTAAATATGTTTTTTTACGAGAAAAACAGATTAAATATGTCACTAAAATTTACTATAAAATAGTAAGGTTCATACTAGATTAAATTCATCATATAAGATCTTTAAAAGGTGTTTTAGAGGCATTCGTTAGATAATGTAATATATCTTTTTTAATGCAGAAATAAATTAAAGCATCATTTATCATTATCTTATTATAATATTTCTGGTGCAACTAATCTTTATTATATTAATTCCTTTGAAAAACTTATTTTATTCTTTTTGGTATTAATTGCCTCTGACATAACAACAGATGGCGAGGTACACACATGCAATGAGAGTGTTGAAGAAGTTGATGATCAGAACAAAAAAATCACATACAAGCTGTTTGGTGGAGATATTGAAAACTATAAGGTCTTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAATTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATGTTGATACTCCAAATGGATGGATGGACTACCTTAGCAAGAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MS.gene59863.t1

MVLAGKLITELGIKTPADKFYKLFASELHEVQNVCERVHHTKLHEGEDWHHTDSVKHWTYVIDGEVHTCNESVEEVDDQNKKITYKLFGGDIENYKVFKLILEVLNKADGNSAVRWTIDYEKNNEDVDTPNGWMDYLSKSTRDIDGHLVKGEKVAL