AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83053


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83053.t1 MTR_1g032560 94.186 172 10 0 1 172 1 172 4.45e-112 316
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83053.t1 AT2G01755 58.475 118 49 0 54 171 56 173 1.58e-42 140
MS.gene83053.t1 AT2G01755 58.475 118 49 0 54 171 62 179 1.97e-42 140

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTCTATAGATAGAATAAA+AGG 0.213144 1.4:+17113856 MS.gene83053:CDS
ATCAAAGAGGTATAAGCTTT+TGG 0.314010 1.4:+17113345 MS.gene83053:CDS
TAAGTACTTCTACTTCAATT+TGG 0.331886 1.4:-17113511 None:intergenic
GGAAACTTGTCAATTGATAA+TGG 0.335882 1.4:-17113272 None:intergenic
CCATGGCATCATTGTCCATT+TGG 0.363669 1.4:-17113234 None:intergenic
AGAATGCTTCTTGTTGGGTC+AGG 0.383377 1.4:-17113814 None:intergenic
GAAGATAGAGATGATGAGTA+TGG 0.389990 1.4:+17113407 MS.gene83053:CDS
GCTGGAAGAATGCTTCTTGT+TGG 0.394629 1.4:-17113820 None:intergenic
CTTCTGCAGATACTCAATAT+TGG 0.406034 1.4:-17113893 None:intergenic
CTGGAAGAATGCTTCTTGTT+GGG 0.406470 1.4:-17113819 None:intergenic
GACTTTATCACTGCCCTGGC+TGG 0.412026 1.4:-17113838 None:intergenic
TTGTCAATTGATAATGGTAT+AGG 0.420243 1.4:-17113266 None:intergenic
AGTAGAAGTACTTATCTTCA+AGG 0.426944 1.4:+17113520 MS.gene83053:CDS
TGTCCATTTGGCCATACCCT+TGG 0.430122 1.4:-17113222 None:intergenic
TCAATATTGGCAGGGTCAAA+AGG 0.439860 1.4:-17113880 None:intergenic
GTCCATTTGGCCATACCCTT+GGG 0.442034 1.4:-17113221 None:intergenic
CAATGAGGAAATTTCAAAGT+TGG 0.445765 1.4:+17113433 MS.gene83053:CDS
CTCTATCTTCATCCTCATCT+TGG 0.448503 1.4:-17113394 None:intergenic
TGCAGATACTCAATATTGGC+AGG 0.450870 1.4:-17113889 None:intergenic
TTCCCAAGGGTATGGCCAAA+TGG 0.469349 1.4:+17113219 MS.gene83053:CDS
ATACCCTTGGGAAGTGTAGT+AGG 0.478990 1.4:-17113209 None:intergenic
GGACAATGATGCCATGGCCT+AGG 0.481592 1.4:+17113240 MS.gene83053:CDS
CAAGAAGCATTCTTCCAGCC+AGG 0.483504 1.4:+17113824 MS.gene83053:CDS
GAATGCTTCTTGTTGGGTCA+GGG 0.497042 1.4:-17113813 None:intergenic
CACAAGCTTACATTGTGAGT+TGG 0.506810 1.4:-17113293 None:intergenic
AGTCTATAGATAGAATAAAA+GGG 0.523947 1.4:+17113857 MS.gene83053:CDS
AATGCTTCTTGTTGGGTCAG+GGG 0.544161 1.4:-17113812 None:intergenic
TACTACACTTCCCAAGGGTA+TGG 0.545556 1.4:+17113211 MS.gene83053:CDS
TTTGTCTTGAATTCTTCCCA+TGG 0.554335 1.4:-17113922 None:intergenic
ATCTGCAGAAGGATGTTCCA+TGG 0.555118 1.4:+17113905 MS.gene83053:CDS
GATAATGGTATAGGTAGCCT+AGG 0.559854 1.4:-17113257 None:intergenic
TATAGACTTTATCACTGCCC+TGG 0.571801 1.4:-17113842 None:intergenic
ACTCATTGTTCATGCACTTG+TGG 0.576626 1.4:+17113487 MS.gene83053:CDS
GCATCCTACTACACTTCCCA+AGG 0.594031 1.4:+17113205 MS.gene83053:CDS
AAGAAGCATTCTTCCAGCCA+GGG 0.602074 1.4:+17113825 MS.gene83053:CDS
TTCTATAGTCAATCAGCAAG+AGG 0.607447 1.4:+17113458 MS.gene83053:CDS
AGGGGAAGTACCATAGCTCA+AGG 0.608247 1.4:-17113794 None:intergenic
TCTGCAGAAGGATGTTCCAT+GGG 0.613447 1.4:+17113906 MS.gene83053:CDS
CAATATTGAGTATCTGCAGA+AGG 0.617485 1.4:+17113894 MS.gene83053:CDS
CATCCTACTACACTTCCCAA+GGG 0.625588 1.4:+17113206 MS.gene83053:CDS
CCAAATGGACAATGATGCCA+TGG 0.626699 1.4:+17113234 MS.gene83053:CDS
GGTATAGGTAGCCTAGGCCA+TGG 0.632885 1.4:-17113251 None:intergenic
GCAAATTAGATACCAAGATG+AGG 0.635629 1.4:+17113382 MS.gene83053:CDS
TTAGATTTGAGCAATCAAAG+AGG 0.645074 1.4:+17113332 MS.gene83053:CDS
GCAGATACTCAATATTGGCA+GGG 0.648369 1.4:-17113888 None:intergenic
TGATGAGTATGGACACAATG+AGG 0.651393 1.4:+17113418 MS.gene83053:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATTTGTTTGTTTTAT+TGG - chr1.4:17113599-17113618 None:intergenic 10.0%
!!! TTTTTTTTCTAATTAGAAAA+AGG + chr1.4:17113640-17113659 MS.gene83053:intron 10.0%
!! AAGTCTATAGATAGAATAAA+AGG + chr1.4:17113856-17113875 MS.gene83053:CDS 20.0%
!! AGTCTATAGATAGAATAAAA+GGG + chr1.4:17113857-17113876 MS.gene83053:CDS 20.0%
!!! TTGTTTGTTTTATTGGTTAT+TGG - chr1.4:17113592-17113611 None:intergenic 20.0%
! CCATTTGCAAAACAAAAATT+TGG - chr1.4:17113697-17113716 None:intergenic 25.0%
! TAAGTACTTCTACTTCAATT+TGG - chr1.4:17113514-17113533 None:intergenic 25.0%
!!! CCAAATTTTTGTTTTGCAAA+TGG + chr1.4:17113694-17113713 MS.gene83053:intron 25.0%
!!! TTGTCAATTGATAATGGTAT+AGG - chr1.4:17113269-17113288 None:intergenic 25.0%
ACTTATCTTCAAGGTAAGTT+TGG + chr1.4:17113529-17113548 MS.gene83053:intron 30.0%
CAATGAGGAAATTTCAAAGT+TGG + chr1.4:17113433-17113452 MS.gene83053:CDS 30.0%
GGAAACTTGTCAATTGATAA+TGG - chr1.4:17113275-17113294 None:intergenic 30.0%
TGACAAACCATTTCATTTCA+GGG + chr1.4:17113760-17113779 MS.gene83053:intron 30.0%
TTAGATTTGAGCAATCAAAG+AGG + chr1.4:17113332-17113351 MS.gene83053:CDS 30.0%
!! AGTAGAAGTACTTATCTTCA+AGG + chr1.4:17113520-17113539 MS.gene83053:CDS 30.0%
!! ATCAAAGAGGTATAAGCTTT+TGG + chr1.4:17113345-17113364 MS.gene83053:CDS 30.0%
AGAAAACCCCTGAAATGAAA+TGG - chr1.4:17113770-17113789 None:intergenic 35.0%
CAATATTGAGTATCTGCAGA+AGG + chr1.4:17113894-17113913 MS.gene83053:CDS 35.0%
CTTCTGCAGATACTCAATAT+TGG - chr1.4:17113896-17113915 None:intergenic 35.0%
GAAGATAGAGATGATGAGTA+TGG + chr1.4:17113407-17113426 MS.gene83053:CDS 35.0%
GACAAACCATTTCATTTCAG+GGG + chr1.4:17113761-17113780 MS.gene83053:intron 35.0%
GCAAATTAGATACCAAGATG+AGG + chr1.4:17113382-17113401 MS.gene83053:CDS 35.0%
GTGACAAACCATTTCATTTC+AGG + chr1.4:17113759-17113778 MS.gene83053:intron 35.0%
TTCTATAGTCAATCAGCAAG+AGG + chr1.4:17113458-17113477 MS.gene83053:CDS 35.0%
TTTGTCTTGAATTCTTCCCA+TGG - chr1.4:17113925-17113944 None:intergenic 35.0%
! TTTTCTTCATCCTTGAGCTA+TGG + chr1.4:17113784-17113803 MS.gene83053:CDS 35.0%
ACTCATTGTTCATGCACTTG+TGG + chr1.4:17113487-17113506 MS.gene83053:CDS 40.0%
CACAAGCTTACATTGTGAGT+TGG - chr1.4:17113296-17113315 None:intergenic 40.0%
CTCTATCTTCATCCTCATCT+TGG - chr1.4:17113397-17113416 None:intergenic 40.0%
CTGGAAGAATGCTTCTTGTT+GGG - chr1.4:17113822-17113841 None:intergenic 40.0%
GCAGATACTCAATATTGGCA+GGG - chr1.4:17113891-17113910 None:intergenic 40.0%
TATAGACTTTATCACTGCCC+TGG - chr1.4:17113845-17113864 None:intergenic 40.0%
TCAATATTGGCAGGGTCAAA+AGG - chr1.4:17113883-17113902 None:intergenic 40.0%
TGATGAGTATGGACACAATG+AGG + chr1.4:17113418-17113437 MS.gene83053:CDS 40.0%
TGCAGATACTCAATATTGGC+AGG - chr1.4:17113892-17113911 None:intergenic 40.0%
! GATAATGGTATAGGTAGCCT+AGG - chr1.4:17113260-17113279 None:intergenic 40.0%
!! AGAGGTATAAGCTTTTGGTG+TGG + chr1.4:17113350-17113369 MS.gene83053:CDS 40.0%
AATGCTTCTTGTTGGGTCAG+GGG - chr1.4:17113815-17113834 None:intergenic 45.0%
AGAATGCTTCTTGTTGGGTC+AGG - chr1.4:17113817-17113836 None:intergenic 45.0%
ATCTGCAGAAGGATGTTCCA+TGG + chr1.4:17113905-17113924 MS.gene83053:CDS 45.0%
CATCCTACTACACTTCCCAA+GGG + chr1.4:17113206-17113225 MS.gene83053:CDS 45.0%
CCAAATGGACAATGATGCCA+TGG + chr1.4:17113234-17113253 MS.gene83053:CDS 45.0%
CCATGGCATCATTGTCCATT+TGG - chr1.4:17113237-17113256 None:intergenic 45.0%
GAATGCTTCTTGTTGGGTCA+GGG - chr1.4:17113816-17113835 None:intergenic 45.0%
GCTGGAAGAATGCTTCTTGT+TGG - chr1.4:17113823-17113842 None:intergenic 45.0%
TACTACACTTCCCAAGGGTA+TGG + chr1.4:17113211-17113230 MS.gene83053:CDS 45.0%
TCTGCAGAAGGATGTTCCAT+GGG + chr1.4:17113906-17113925 MS.gene83053:CDS 45.0%
! ATACCCTTGGGAAGTGTAGT+AGG - chr1.4:17113212-17113231 None:intergenic 45.0%
!! AAGAAGCATTCTTCCAGCCA+GGG + chr1.4:17113825-17113844 MS.gene83053:CDS 45.0%
!! TAAGCTTTTGGTGTGGAGCA+AGG + chr1.4:17113357-17113376 MS.gene83053:CDS 45.0%
GCATCCTACTACACTTCCCA+AGG + chr1.4:17113205-17113224 MS.gene83053:CDS 50.0%
GTCCATTTGGCCATACCCTT+GGG - chr1.4:17113224-17113243 None:intergenic 50.0%
TGTCCATTTGGCCATACCCT+TGG - chr1.4:17113225-17113244 None:intergenic 50.0%
! TTCCCAAGGGTATGGCCAAA+TGG + chr1.4:17113219-17113238 MS.gene83053:CDS 50.0%
!! AGGGGAAGTACCATAGCTCA+AGG - chr1.4:17113797-17113816 None:intergenic 50.0%
!! CAAGAAGCATTCTTCCAGCC+AGG + chr1.4:17113824-17113843 MS.gene83053:CDS 50.0%
GACTTTATCACTGCCCTGGC+TGG - chr1.4:17113841-17113860 None:intergenic 55.0%
GGACAATGATGCCATGGCCT+AGG + chr1.4:17113240-17113259 MS.gene83053:CDS 55.0%
GGTATAGGTAGCCTAGGCCA+TGG - chr1.4:17113254-17113273 None:intergenic 55.0%
!! GCTTTTGGTGTGGAGCAAGG+AGG + chr1.4:17113360-17113379 MS.gene83053:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 17113203 17113960 17113203 ID=MS.gene83053
chr1.4 mRNA 17113203 17113960 17113203 ID=MS.gene83053.t1;Parent=MS.gene83053
chr1.4 exon 17113203 17113541 17113203 ID=MS.gene83053.t1.exon1;Parent=MS.gene83053.t1
chr1.4 CDS 17113203 17113541 17113203 ID=cds.MS.gene83053.t1;Parent=MS.gene83053.t1
chr1.4 exon 17113781 17113960 17113781 ID=MS.gene83053.t1.exon2;Parent=MS.gene83053.t1
chr1.4 CDS 17113781 17113960 17113781 ID=cds.MS.gene83053.t1;Parent=MS.gene83053.t1
Gene Sequence

>MS.gene83053

ATGCATCCTACTACACTTCCCAAGGGTATGGCCAAATGGACAATGATGCCATGGCCTAGGCTACCTATACCATTATCAATTGACAAGTTTCCAACTCACAATGTAAGCTTGTGTTTTGACTTGAAAGAATTAGATTTGAGCAATCAAAGAGGTATAAGCTTTTGGTGTGGAGCAAGGAGGCAAATTAGATACCAAGATGAGGATGAAGATAGAGATGATGAGTATGGACACAATGAGGAAATTTCAAAGTTGGAATTCTATAGTCAATCAGCAAGAGGAGAAGCACTCATTGTTCATGCACTTGTGGACCAAATTGAAGTAGAAGTACTTATCTTCAAGGTAAGTTTGGAATTTATGTCAATAATTGCTTTTAAGAGTCTTGCTATCCAATAACCAATAAAACAAACAAATTATTTAAAAATATATGAATCTACTTCTTTTTTTTCTAATTAGAAAAAGGTAAGAAGCACATTAAAATTCTTGTGATTTTACCAAATTTTTGTTTTGCAAATGGTATGCTGATTACTCTAAAAATATATTTCTTGATAGAAATTTAGTGACAAACCATTTCATTTCAGGGGTTTTCTTCATCCTTGAGCTATGGTACTTCCCCTGACCCAACAAGAAGCATTCTTCCAGCCAGGGCAGTGATAAAGTCTATAGATAGAATAAAAGGGCCTTTTGACCCTGCCAATATTGAGTATCTGCAGAAGGATGTTCCATGGGAAGAATTCAAGACAAATATTCTTTCTAGCTAA

Protein sequence

>MS.gene83053.t1

MHPTTLPKGMAKWTMMPWPRLPIPLSIDKFPTHNVSLCFDLKELDLSNQRGISFWCGARRQIRYQDEDEDRDDEYGHNEEISKLEFYSQSARGEALIVHALVDQIEVEVLIFKGFSSSLSYGTSPDPTRSILPARAVIKSIDRIKGPFDPANIEYLQKDVPWEEFKTNILSS