AlfalfaGEDB | MS.gene83056

M. sativa cultivar XinJiangDaYe / MS.gene83056

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0006996 GO:0007010 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015631 GO:0016043 GO:0071840
PFAM: PF07058.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83056.t1	GAU32991.1	65.6	131	11	2	1	129	1	99	1.40E-31	146

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83056.t1	Q9LQU7	47.5	122	31	2	9	129	5	94	2.4e-16	86.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83056.t1	A0A2Z6MNL5	65.6	131	11	2	1	129	1	99	1.0e-31	146.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene83056	0.839506	1.52E-57	-1.69E-46
MS.gene049159	MS.gene83056	0.860135	2.55E-63	-1.69E-46
MS.gene049368	MS.gene83056	0.816638	4.99E-52	-1.69E-46
MS.gene049382	MS.gene83056	0.820538	6.51E-53	-1.69E-46
MS.gene049435	MS.gene83056	0.818044	2.41E-52	-1.69E-46
MS.gene049850	MS.gene83056	0.802763	4.81E-49	-1.69E-46
MS.gene049983	MS.gene83056	0.808837	2.55E-50	-1.69E-46
MS.gene050020	MS.gene83056	0.819263	1.27E-52	-1.69E-46
MS.gene050084	MS.gene83056	0.802337	5.88E-49	-1.69E-46
MS.gene050085	MS.gene83056	0.824218	9.09E-54	-1.69E-46
MS.gene050361	MS.gene83056	0.809216	2.11E-50	-1.69E-46
MS.gene050384	MS.gene83056	0.814742	1.32E-51	-1.69E-46
MS.gene050386	MS.gene83056	0.811919	5.50E-51	-1.69E-46
MS.gene050551	MS.gene83056	0.850927	1.24E-60	-1.69E-46
MS.gene050654	MS.gene83056	0.813829	2.10E-51	-1.69E-46
MS.gene050689	MS.gene83056	0.850347	1.80E-60	-1.69E-46
MS.gene050762	MS.gene83056	0.818153	2.27E-52	-1.69E-46
MS.gene050763	MS.gene83056	0.818231	2.18E-52	-1.69E-46
MS.gene050807	MS.gene83056	0.848543	5.73E-60	-1.69E-46
MS.gene050808	MS.gene83056	0.803405	3.54E-49	-1.69E-46
MS.gene050815	MS.gene83056	0.80094	1.14E-48	-1.69E-46
MS.gene050826	MS.gene83056	0.826744	2.29E-54	-1.69E-46
MS.gene051066	MS.gene83056	0.806553	7.78E-50	-1.69E-46
MS.gene051102	MS.gene83056	0.837765	4.29E-57	-1.69E-46
MS.gene051180	MS.gene83056	0.822287	2.57E-53	-1.69E-46
MS.gene051198	MS.gene83056	0.833251	5.94E-56	-1.69E-46
MS.gene051328	MS.gene83056	0.802306	5.97E-49	-1.69E-46
MS.gene051381	MS.gene83056	0.803195	3.91E-49	-1.69E-46
MS.gene05157	MS.gene83056	0.819086	1.40E-52	-1.69E-46
MS.gene051719	MS.gene83056	0.81191	5.53E-51	-1.69E-46
MS.gene051720	MS.gene83056	0.809237	2.09E-50	-1.69E-46
MS.gene051800	MS.gene83056	0.829184	5.93E-55	-1.69E-46
MS.gene051856	MS.gene83056	0.802518	5.40E-49	-1.69E-46
MS.gene052012	MS.gene83056	0.80588	1.08E-49	-1.69E-46
MS.gene052020	MS.gene83056	0.810804	9.61E-51	-1.69E-46
MS.gene05215	MS.gene83056	0.806543	7.82E-50	-1.69E-46
MS.gene052307	MS.gene83056	0.838868	2.23E-57	-1.69E-46
MS.gene052323	MS.gene83056	0.828843	7.18E-55	-1.69E-46
MS.gene052397	MS.gene83056	0.83608	1.15E-56	-1.69E-46
MS.gene052463	MS.gene83056	0.802714	4.92E-49	-1.69E-46
MS.gene052516	MS.gene83056	0.841025	6.10E-58	-1.69E-46
MS.gene052609	MS.gene83056	0.843006	1.83E-58	-1.69E-46
MS.gene052611	MS.gene83056	0.822328	2.51E-53	-1.69E-46
MS.gene052665	MS.gene83056	0.844784	6.10E-59	-1.69E-46
MS.gene052776	MS.gene83056	0.808711	2.71E-50	-1.69E-46
MS.gene052860	MS.gene83056	0.800269	1.56E-48	-1.69E-46
MS.gene052863	MS.gene83056	0.828517	8.60E-55	-1.69E-46
MS.gene052896	MS.gene83056	0.833288	5.81E-56	-1.69E-46
MS.gene052994	MS.gene83056	0.824919	6.22E-54	-1.69E-46
MS.gene053008	MS.gene83056	0.836804	7.55E-57	-1.69E-46
MS.gene053079	MS.gene83056	0.849131	3.94E-60	-1.69E-46
MS.gene053226	MS.gene83056	0.815057	1.12E-51	-1.69E-46
MS.gene053563	MS.gene83056	0.808295	3.32E-50	-1.69E-46
MS.gene053581	MS.gene83056	0.813129	2.99E-51	-1.69E-46
MS.gene05364	MS.gene83056	0.825424	4.72E-54	-1.69E-46
MS.gene054379	MS.gene83056	0.829612	4.67E-55	-1.69E-46
MS.gene054472	MS.gene83056	0.806337	8.64E-50	-1.69E-46
MS.gene05458	MS.gene83056	0.801344	9.41E-49	-1.69E-46
MS.gene05462	MS.gene83056	0.82024	7.62E-53	-1.69E-46
MS.gene054823	MS.gene83056	0.826573	2.52E-54	-1.69E-46
MS.gene055227	MS.gene83056	0.808261	3.38E-50	-1.69E-46
MS.gene055310	MS.gene83056	0.80104	1.09E-48	-1.69E-46
MS.gene055311	MS.gene83056	0.80859	2.87E-50	-1.69E-46
MS.gene055313	MS.gene83056	0.819489	1.13E-52	-1.69E-46
MS.gene055465	MS.gene83056	0.812454	4.21E-51	-1.69E-46
MS.gene055466	MS.gene83056	0.802677	5.01E-49	-1.69E-46
MS.gene055469	MS.gene83056	0.809237	2.09E-50	-1.69E-46
MS.gene055512	MS.gene83056	0.831133	1.98E-55	-1.69E-46
MS.gene055513	MS.gene83056	0.820118	8.12E-53	-1.69E-46
MS.gene055926	MS.gene83056	0.829515	4.93E-55	-1.69E-46
MS.gene056045	MS.gene83056	0.824763	6.77E-54	-1.69E-46
MS.gene05604	MS.gene83056	0.817021	4.09E-52	-1.69E-46
MS.gene056181	MS.gene83056	0.854907	9.00E-62	-1.69E-46
MS.gene056308	MS.gene83056	0.840253	9.72E-58	-1.69E-46
MS.gene056480	MS.gene83056	0.808848	2.53E-50	-1.69E-46
MS.gene056507	MS.gene83056	0.82809	1.09E-54	-1.69E-46
MS.gene056553	MS.gene83056	0.810863	9.33E-51	-1.69E-46
MS.gene056760	MS.gene83056	0.812691	3.73E-51	-1.69E-46
MS.gene057034	MS.gene83056	0.818366	2.04E-52	-1.69E-46
MS.gene057360	MS.gene83056	0.804685	1.92E-49	-1.69E-46
MS.gene057444	MS.gene83056	0.813671	2.27E-51	-1.69E-46
MS.gene057446	MS.gene83056	0.839016	2.04E-57	-1.69E-46
MS.gene057855	MS.gene83056	0.802477	5.51E-49	-1.69E-46
MS.gene057869	MS.gene83056	0.837227	5.89E-57	-1.69E-46
MS.gene057915	MS.gene83056	0.844543	7.08E-59	-1.69E-46
MS.gene058010	MS.gene83056	0.850034	2.20E-60	-1.69E-46
MS.gene058292	MS.gene83056	0.832411	9.60E-56	-1.69E-46
MS.gene058595	MS.gene83056	0.814525	1.47E-51	-1.69E-46
MS.gene058636	MS.gene83056	0.800877	1.17E-48	-1.69E-46
MS.gene058638	MS.gene83056	0.819198	1.32E-52	-1.69E-46
MS.gene058639	MS.gene83056	0.828713	7.72E-55	-1.69E-46
MS.gene058646	MS.gene83056	0.827337	1.65E-54	-1.69E-46
MS.gene058647	MS.gene83056	0.820591	6.33E-53	-1.69E-46
MS.gene058919	MS.gene83056	0.817562	3.09E-52	-1.69E-46
MS.gene058979	MS.gene83056	0.818624	1.78E-52	-1.69E-46
MS.gene059171	MS.gene83056	0.802251	6.13E-49	-1.69E-46
MS.gene059331	MS.gene83056	0.818286	2.12E-52	-1.69E-46
MS.gene059333	MS.gene83056	0.802026	6.82E-49	-1.69E-46
MS.gene059335	MS.gene83056	0.810283	1.24E-50	-1.69E-46
MS.gene059439	MS.gene83056	0.806287	8.85E-50	-1.69E-46
MS.gene059713	MS.gene83056	0.821424	4.07E-53	-1.69E-46
MS.gene059725	MS.gene83056	0.840913	6.53E-58	-1.69E-46
MS.gene05978	MS.gene83056	0.848164	7.30E-60	-1.69E-46
MS.gene059842	MS.gene83056	0.838023	3.68E-57	-1.69E-46
MS.gene059937	MS.gene83056	0.814162	1.77E-51	-1.69E-46
MS.gene060296	MS.gene83056	0.839993	1.14E-57	-1.69E-46
MS.gene060310	MS.gene83056	0.808031	3.78E-50	-1.69E-46
MS.gene06052	MS.gene83056	0.817608	3.02E-52	-1.69E-46
MS.gene060587	MS.gene83056	0.815468	9.11E-52	-1.69E-46
MS.gene060637	MS.gene83056	0.840503	8.36E-58	-1.69E-46
MS.gene060683	MS.gene83056	0.809871	1.53E-50	-1.69E-46
MS.gene060685	MS.gene83056	0.814501	1.49E-51	-1.69E-46
MS.gene060802	MS.gene83056	0.815515	8.89E-52	-1.69E-46
MS.gene060803	MS.gene83056	0.841569	4.39E-58	-1.69E-46
MS.gene060935	MS.gene83056	0.801185	1.01E-48	-1.69E-46
MS.gene060998	MS.gene83056	0.810345	1.21E-50	-1.69E-46
MS.gene061006	MS.gene83056	0.838532	2.72E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83056.t1	MTR_1g032520	71.852	135	6	1	1	135	1	103	2.99e-53	177
MS.gene83056.t1	MTR_8g105350	55.714	140	25	3	1	135	1	108	7.85e-36	130
MS.gene83056.t1	MTR_8g021120	59.854	137	20	3	1	135	1	104	8.88e-34	124

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83056.t1	AT1G14840	50.413	121	19	4	17	132	13	97	1.39e-23	95.9
MS.gene83056.t1	AT1G14840	50.413	121	19	4	17	132	13	97	1.82e-23	95.5
MS.gene83056.t1	AT1G68060	45.205	146	28	6	1	129	1	111	7.78e-22	90.9
MS.gene83056.t1	AT2G01750	50.427	117	21	3	21	132	37	121	1.02e-20	87.4
MS.gene83056.t1	AT2G01750	50.427	117	21	3	21	132	37	121	1.03e-20	87.4

Find 19 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTAAACGATCCAGAAAA+TGG	0.341450	1.2:-17036534	None:intergenic
TTCTGCTAGACAGCAGAGTA+TGG	0.375230	1.2:+17036584	MS.gene83056:CDS
CAGTGAAAGTGGAGCTTAAT+CGG	0.377411	1.2:+17036646	MS.gene83056:CDS
CATGAATTTGTTGCACGGTT+CGG	0.387547	1.2:+17036620	MS.gene83056:CDS
AGATAAGGATAGAGAATTAT+CGG	0.413036	1.2:+17036782	MS.gene83056:CDS
ACGGCGATGGAGGAGATTTA+CGG	0.436994	1.2:+17036483	None:intergenic
CGATTAAGCTCCACTTTCAC+TGG	0.472787	1.2:-17036645	None:intergenic
CGGAGGAGCGGTGGCGACGC+CGG	0.479934	1.2:+17036509	MS.gene83056:CDS
CGGTTCGGATCCAGTGAAAG+TGG	0.533833	1.2:+17036635	MS.gene83056:CDS
AACGATCCAGAAAATGGTGC+CGG	0.568721	1.2:-17036528	None:intergenic
CGGCTTGAGAATGAAGTTCG+AGG	0.589218	1.2:+17036666	MS.gene83056:CDS
AAGCGTTGAGGCATTCTGAA+CGG	0.613362	1.2:+17036823	MS.gene83056:CDS
TTACGGCGACGGAGGAGCGG+TGG	0.622998	1.2:+17036500	MS.gene83056:CDS
GAGGAGATTTACGGCGACGG+AGG	0.631996	1.2:+17036492	MS.gene83056:CDS
GAGTTCATGAATTTGTTGCA+CGG	0.637392	1.2:+17036615	MS.gene83056:CDS
GATTTACGGCGACGGAGGAG+CGG	0.644818	1.2:+17036497	MS.gene83056:CDS
ATGGAGGAGATTTACGGCGA+CGG	0.663204	1.2:+17036489	MS.gene83056:CDS
AAGCTGAGATTAAAGCGTTG+AGG	0.667550	1.2:+17036811	MS.gene83056:CDS
AGAACTTCGTCGAGAAGACG+CGG	0.686070	1.2:+17036561	MS.gene83056:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTCAATTGTTGTTATTTT+TGG	+	chr1.2:17036882-17036901	MS.gene83056:CDS	20.0%
!	AGATAAGGATAGAGAATTAT+CGG	+	chr1.2:17036782-17036801	MS.gene83056:CDS	25.0%
!!!	TGTTTGTTTTTGTGTAGATA+AGG	+	chr1.2:17036767-17036786	MS.gene83056:CDS	25.0%
	AATTCAGCTGAAAAACTCTT+CGG	-	chr1.2:17036711-17036730	None:intergenic	30.0%
	TCTCTAAACGATCCAGAAAA+TGG	-	chr1.2:17036537-17036556	None:intergenic	35.0%
!	TTTTCTGGATCGTTTAGAGA+AGG	+	chr1.2:17036537-17036556	MS.gene83056:CDS	35.0%
!!	GAGTTCATGAATTTGTTGCA+CGG	+	chr1.2:17036615-17036634	MS.gene83056:CDS	35.0%
	CAGTGAAAGTGGAGCTTAAT+CGG	+	chr1.2:17036646-17036665	MS.gene83056:CDS	40.0%
	GAGAGAAAAAGCTGTTGAAG+AGG	+	chr1.2:17036848-17036867	MS.gene83056:CDS	40.0%
!!	AAGCTGAGATTAAAGCGTTG+AGG	+	chr1.2:17036811-17036830	MS.gene83056:CDS	40.0%
!!	CATGAATTTGTTGCACGGTT+CGG	+	chr1.2:17036620-17036639	MS.gene83056:CDS	40.0%
	AACGATCCAGAAAATGGTGC+CGG	-	chr1.2:17036531-17036550	None:intergenic	45.0%
	CGATTAAGCTCCACTTTCAC+TGG	-	chr1.2:17036648-17036667	None:intergenic	45.0%
	TTCTGCTAGACAGCAGAGTA+TGG	+	chr1.2:17036584-17036603	MS.gene83056:CDS	45.0%
!!	AAGCGTTGAGGCATTCTGAA+CGG	+	chr1.2:17036823-17036842	MS.gene83056:CDS	45.0%
!!!	AATTTTACTTAATTTTTTTT+TGG	+	chr1.2:17036727-17036746	MS.gene83056:CDS	5.0%
	AGAACTTCGTCGAGAAGACG+CGG	+	chr1.2:17036561-17036580	MS.gene83056:CDS	50.0%
	ATGGAGGAGATTTACGGCGA+CGG	+	chr1.2:17036489-17036508	MS.gene83056:CDS	50.0%
	CGGCTTGAGAATGAAGTTCG+AGG	+	chr1.2:17036666-17036685	MS.gene83056:CDS	50.0%
!	CGGTTCGGATCCAGTGAAAG+TGG	+	chr1.2:17036635-17036654	MS.gene83056:CDS	55.0%
	GAGGAGATTTACGGCGACGG+AGG	+	chr1.2:17036492-17036511	MS.gene83056:CDS	60.0%
	GATTTACGGCGACGGAGGAG+CGG	+	chr1.2:17036497-17036516	MS.gene83056:CDS	60.0%
!	GCGACGCCGGCACCATTTTC+TGG	+	chr1.2:17036522-17036541	MS.gene83056:CDS	65.0%
	TTACGGCGACGGAGGAGCGG+TGG	+	chr1.2:17036500-17036519	MS.gene83056:CDS	70.0%
!	CGGAGGAGCGGTGGCGACGC+CGG	+	chr1.2:17036509-17036528	MS.gene83056:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	17036489	17036929	17036489	ID=MS.gene83056
chr1.2	mRNA	17036489	17036929	17036489	ID=MS.gene83056.t1;Parent=MS.gene83056
chr1.2	exon	17036489	17036929	17036489	ID=MS.gene83056.t1.exon1;Parent=MS.gene83056.t1
chr1.2	CDS	17036489	17036929	17036489	ID=cds.MS.gene83056.t1;Parent=MS.gene83056.t1

Gene Sequence

>MS.gene83056

ATGGAGGAGATTTACGGCGACGGAGGAGCGGTGGCGACGCCGGCACCATTTTCTGGATCGTTTAGAGAAGGTAGAACTTCGTCGAGAAGACGCGGTTCTGCTAGACAGCAGAGTATGGATGCTGATGAGTTCATGAATTTGTTGCACGGTTCGGATCCAGTGAAAGTGGAGCTTAATCGGCTTGAGAATGAAGTTCGAGGTGCGAAAATGTTGCTCGATCCGAAGAGTTTTTCAGCTGAATTTTACTTAATTTTTTTTTGGATTTTTTTTTTAATTTGTGTTTGTTTTTGTGTAGATAAGGATAGAGAATTATCGGAAGCACAAGCTGAGATTAAAGCGTTGAGGCATTCTGAACGGTTGAGAGAAAAAGCTGTTGAAGAGGTTACTTTTGATTTCTCAATTGTTGTTATTTTTGGCGTTATTGATGTTTTTATTGTTTGA

Protein sequence

>MS.gene83056.t1

MEEIYGDGGAVATPAPFSGSFREGRTSSRRRGSARQQSMDADEFMNLLHGSDPVKVELNRLENEVRGAKMLLDPKSFSAEFYLIFFWIFFLICVCFCVDKDRELSEAQAEIKALRHSERLREKAVEEVTFDFSIVVIFGVIDVFIV