Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90239.t1 | AES90567.2 | 98.8 | 82 | 1 | 0 | 1 | 82 | 132 | 213 | 9.90E-35 | 155.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90239.t1 | G7JU33 | 98.8 | 82 | 1 | 0 | 1 | 82 | 132 | 213 | 7.1e-35 | 155.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene90239 | 0.81142 | 7.07E-51 | -1.69E-46 |
| MS.gene049190 | MS.gene90239 | 0.81622 | 6.19E-52 | -1.69E-46 |
| MS.gene049254 | MS.gene90239 | 0.828228 | 1.01E-54 | -1.69E-46 |
| MS.gene049271 | MS.gene90239 | 0.811228 | 7.78E-51 | -1.69E-46 |
| MS.gene049341 | MS.gene90239 | 0.821778 | 3.37E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene90239 | 0.815268 | 1.01E-51 | -1.69E-46 |
| MS.gene049404 | MS.gene90239 | 0.855515 | 5.99E-62 | -1.69E-46 |
| MS.gene049501 | MS.gene90239 | 0.801409 | 9.13E-49 | -1.69E-46 |
| MS.gene049711 | MS.gene90239 | 0.825636 | 4.21E-54 | -1.69E-46 |
| MS.gene049807 | MS.gene90239 | 0.800195 | 1.62E-48 | -1.69E-46 |
| MS.gene049821 | MS.gene90239 | 0.822333 | 2.51E-53 | -1.69E-46 |
| MS.gene049824 | MS.gene90239 | 0.832738 | 7.97E-56 | -1.69E-46 |
| MS.gene049825 | MS.gene90239 | 0.817545 | 3.12E-52 | -1.69E-46 |
| MS.gene049850 | MS.gene90239 | 0.820736 | 5.86E-53 | -1.69E-46 |
| MS.gene049873 | MS.gene90239 | 0.83082 | 2.37E-55 | -1.69E-46 |
| MS.gene050020 | MS.gene90239 | 0.823838 | 1.12E-53 | -1.69E-46 |
| MS.gene05016 | MS.gene90239 | 0.817407 | 3.35E-52 | -1.69E-46 |
| MS.gene05045 | MS.gene90239 | 0.804008 | 2.65E-49 | -1.69E-46 |
| MS.gene050559 | MS.gene90239 | 0.836254 | 1.04E-56 | -1.69E-46 |
| MS.gene050561 | MS.gene90239 | 0.836822 | 7.47E-57 | -1.69E-46 |
| MS.gene050562 | MS.gene90239 | 0.824733 | 6.88E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene90239 | 0.843065 | 1.76E-58 | -1.69E-46 |
| MS.gene050808 | MS.gene90239 | 0.807941 | 3.95E-50 | -1.69E-46 |
| MS.gene050901 | MS.gene90239 | 0.810646 | 1.04E-50 | -1.69E-46 |
| MS.gene051247 | MS.gene90239 | 0.855918 | 4.57E-62 | -1.69E-46 |
| MS.gene051279 | MS.gene90239 | 0.841872 | 3.65E-58 | -1.69E-46 |
| MS.gene051328 | MS.gene90239 | 0.807869 | 4.09E-50 | -1.69E-46 |
| MS.gene051388 | MS.gene90239 | 0.806955 | 6.40E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene90239 | 0.83274 | 7.96E-56 | -1.69E-46 |
| MS.gene051594 | MS.gene90239 | 0.805354 | 1.39E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene90239 | 0.823338 | 1.46E-53 | -1.69E-46 |
| MS.gene051719 | MS.gene90239 | 0.853238 | 2.73E-61 | -1.69E-46 |
| MS.gene051720 | MS.gene90239 | 0.877306 | 6.98E-69 | -1.69E-46 |
| MS.gene051744 | MS.gene90239 | 0.85485 | 9.35E-62 | -1.69E-46 |
| MS.gene051957 | MS.gene90239 | 0.819395 | 1.19E-52 | -1.69E-46 |
| MS.gene052020 | MS.gene90239 | 0.811604 | 6.44E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene90239 | 0.813127 | 3.00E-51 | -1.69E-46 |
| MS.gene052239 | MS.gene90239 | -0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene052516 | MS.gene90239 | 0.831059 | 2.07E-55 | -1.69E-46 |
| MS.gene052519 | MS.gene90239 | 0.823494 | 1.34E-53 | -1.69E-46 |
| MS.gene052582 | MS.gene90239 | 0.817815 | 2.71E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene90239 | 0.82262 | 2.15E-53 | -1.69E-46 |
| MS.gene052611 | MS.gene90239 | 0.816183 | 6.31E-52 | -1.69E-46 |
| MS.gene052613 | MS.gene90239 | 0.803918 | 2.77E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene90239 | 0.825056 | 5.77E-54 | -1.69E-46 |
| MS.gene052773 | MS.gene90239 | 0.825545 | 4.42E-54 | -1.69E-46 |
| MS.gene052879 | MS.gene90239 | 0.808541 | 2.94E-50 | -1.69E-46 |
| MS.gene052896 | MS.gene90239 | 0.804571 | 2.03E-49 | -1.69E-46 |
| MS.gene053079 | MS.gene90239 | 0.802394 | 5.73E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene90239 | 0.821672 | 3.57E-53 | -1.69E-46 |
| MS.gene053221 | MS.gene90239 | 0.813451 | 2.54E-51 | -1.69E-46 |
| MS.gene053226 | MS.gene90239 | 0.869854 | 2.27E-66 | -1.69E-46 |
| MS.gene053581 | MS.gene90239 | 0.823376 | 1.43E-53 | -1.69E-46 |
| MS.gene053597 | MS.gene90239 | 0.852586 | 4.19E-61 | -1.69E-46 |
| MS.gene053599 | MS.gene90239 | 0.897717 | 1.07E-76 | -1.69E-46 |
| MS.gene05364 | MS.gene90239 | 0.832076 | 1.16E-55 | -1.69E-46 |
| MS.gene053883 | MS.gene90239 | 0.809506 | 1.83E-50 | -1.69E-46 |
| MS.gene053998 | MS.gene90239 | 0.800226 | 1.59E-48 | -1.69E-46 |
| MS.gene054584 | MS.gene90239 | 0.828056 | 1.11E-54 | -1.69E-46 |
| MS.gene05458 | MS.gene90239 | 0.805982 | 1.03E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene90239 | 0.828241 | 1.00E-54 | -1.69E-46 |
| MS.gene054775 | MS.gene90239 | 0.814138 | 1.79E-51 | -1.69E-46 |
| MS.gene054967 | MS.gene90239 | 0.805952 | 1.04E-49 | -1.69E-46 |
| MS.gene05517 | MS.gene90239 | 0.80234 | 5.88E-49 | -1.69E-46 |
| MS.gene055465 | MS.gene90239 | 0.84753 | 1.09E-59 | -1.69E-46 |
| MS.gene055466 | MS.gene90239 | 0.859846 | 3.12E-63 | -1.69E-46 |
| MS.gene055468 | MS.gene90239 | 0.848195 | 7.15E-60 | -1.69E-46 |
| MS.gene055512 | MS.gene90239 | 0.834979 | 2.19E-56 | -1.69E-46 |
| MS.gene055537 | MS.gene90239 | 0.810009 | 1.43E-50 | -1.69E-46 |
| MS.gene055580 | MS.gene90239 | -0.835204 | 1.92E-56 | -1.69E-46 |
| MS.gene055694 | MS.gene90239 | 0.809579 | 1.76E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene90239 | 0.826892 | 2.11E-54 | -1.69E-46 |
| MS.gene055808 | MS.gene90239 | 0.811598 | 6.46E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene90239 | 0.816753 | 4.70E-52 | -1.69E-46 |
| MS.gene055890 | MS.gene90239 | 0.819064 | 1.41E-52 | -1.69E-46 |
| MS.gene055891 | MS.gene90239 | 0.814011 | 1.91E-51 | -1.69E-46 |
| MS.gene056005 | MS.gene90239 | 0.819902 | 9.10E-53 | -1.69E-46 |
| MS.gene056043 | MS.gene90239 | -0.814307 | 1.65E-51 | -1.69E-46 |
| MS.gene05604 | MS.gene90239 | 0.823718 | 1.19E-53 | -1.69E-46 |
| MS.gene056050 | MS.gene90239 | 0.836769 | 7.71E-57 | -1.69E-46 |
| MS.gene056308 | MS.gene90239 | 0.826188 | 3.11E-54 | -1.69E-46 |
| MS.gene056366 | MS.gene90239 | 0.84197 | 3.44E-58 | -1.69E-46 |
| MS.gene056553 | MS.gene90239 | 0.812331 | 4.47E-51 | -1.69E-46 |
| MS.gene056595 | MS.gene90239 | 0.816739 | 4.73E-52 | -1.69E-46 |
| MS.gene05686 | MS.gene90239 | 0.805097 | 1.57E-49 | -1.69E-46 |
| MS.gene057060 | MS.gene90239 | 0.833145 | 6.31E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene90239 | 0.800655 | 1.30E-48 | -1.69E-46 |
| MS.gene057314 | MS.gene90239 | 0.801608 | 8.31E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene90239 | 0.814436 | 1.54E-51 | -1.69E-46 |
| MS.gene057693 | MS.gene90239 | 0.810132 | 1.34E-50 | -1.69E-46 |
| MS.gene057705 | MS.gene90239 | 0.800465 | 1.42E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene90239 | 0.826379 | 2.80E-54 | -1.69E-46 |
| MS.gene058037 | MS.gene90239 | 0.807512 | 4.88E-50 | -1.69E-46 |
| MS.gene058216 | MS.gene90239 | 0.801024 | 1.09E-48 | -1.69E-46 |
| MS.gene05847 | MS.gene90239 | 0.800058 | 1.72E-48 | -1.69E-46 |
| MS.gene058625 | MS.gene90239 | 0.852874 | 3.47E-61 | -1.69E-46 |
| MS.gene058636 | MS.gene90239 | 0.801793 | 7.61E-49 | -1.69E-46 |
| MS.gene058668 | MS.gene90239 | 0.801791 | 7.62E-49 | -1.69E-46 |
| MS.gene058829 | MS.gene90239 | 0.806529 | 7.87E-50 | -1.69E-46 |
| MS.gene058852 | MS.gene90239 | 0.800289 | 1.55E-48 | -1.69E-46 |
| MS.gene059015 | MS.gene90239 | 0.837981 | 3.77E-57 | -1.69E-46 |
| MS.gene05954 | MS.gene90239 | 0.82738 | 1.61E-54 | -1.69E-46 |
| MS.gene059625 | MS.gene90239 | 0.818132 | 2.30E-52 | -1.69E-46 |
| MS.gene05978 | MS.gene90239 | 0.809321 | 2.00E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene90239 | 0.804319 | 2.29E-49 | -1.69E-46 |
| MS.gene060365 | MS.gene90239 | 0.822685 | 2.08E-53 | -1.69E-46 |
| MS.gene060429 | MS.gene90239 | 0.838401 | 2.94E-57 | -1.69E-46 |
| MS.gene060519 | MS.gene90239 | 0.822329 | 2.51E-53 | -1.69E-46 |
| MS.gene060521 | MS.gene90239 | 0.819926 | 8.99E-53 | -1.69E-46 |
| MS.gene060587 | MS.gene90239 | 0.865721 | 4.81E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90239.t1 | MTR_4g093150 | 98.780 | 82 | 1 | 0 | 1 | 82 | 132 | 213 | 7.49e-54 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAACTCAAGCAATATCT+TGG | 0.374861 | 4.2:-20083698 | MS.gene90239:CDS |
| TGATGAAGTGATCGAGTCTT+TGG | 0.389665 | 4.2:-20083786 | MS.gene90239:CDS |
| AAGAACTCAAGCAATATCTT+GGG | 0.416257 | 4.2:-20083697 | MS.gene90239:CDS |
| GTGTGTTGGAAGAGCAGTTG+AGG | 0.428235 | 4.2:-20083730 | MS.gene90239:CDS |
| AAGCAATATCTTGGGGAGGA+TGG | 0.455312 | 4.2:-20083689 | MS.gene90239:CDS |
| GTAGTTAGCTCTTCTGAAAA+CGG | 0.463530 | 4.2:-20083602 | MS.gene90239:CDS |
| TCTCGAGGCATGAATCTCAT+AGG | 0.486643 | 4.2:-20083754 | MS.gene90239:CDS |
| TGTGTTGGAAGAGCAGTTGA+GGG | 0.487289 | 4.2:-20083729 | MS.gene90239:CDS |
| AGCAATATCTTGGGGAGGAT+GGG | 0.488013 | 4.2:-20083688 | MS.gene90239:CDS |
| AAGCTGATGTTAGTAGAGAT+AGG | 0.499821 | 4.2:-20083652 | MS.gene90239:CDS |
| TACTAACATCAGCTTCAAGT+TGG | 0.521972 | 4.2:+20083660 | None:intergenic |
| CACAACCTTGGAAACTTACA+AGG | 0.522214 | 4.2:+20083571 | None:intergenic |
| AAGTGATCGAGTCTTTGGAA+TGG | 0.572333 | 4.2:-20083781 | MS.gene90239:CDS |
| TGAATCTCATAGGAGTGTGT+TGG | 0.573968 | 4.2:-20083744 | MS.gene90239:CDS |
| CTTTGGAATGGACGATCTCG+AGG | 0.612708 | 4.2:-20083769 | MS.gene90239:CDS |
| ACCTTGGAAACTTACAAGGT+CGG | 0.615530 | 4.2:+20083575 | None:intergenic |
| AGAACTCAAGCAATATCTTG+GGG | 0.655002 | 4.2:-20083696 | MS.gene90239:CDS |
| ACTCAAGCAATATCTTGGGG+AGG | 0.657753 | 4.2:-20083693 | MS.gene90239:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAACTCAAGCAATATCTT+GGG | - | chr4.2:20083701-20083720 | MS.gene90239:CDS | 30.0% | |
| AAGCTGATGTTAGTAGAGAT+AGG | - | chr4.2:20083746-20083765 | MS.gene90239:CDS | 35.0% | |
| AGAACTCAAGCAATATCTTG+GGG | - | chr4.2:20083702-20083721 | MS.gene90239:CDS | 35.0% | |
| GAAGAACTCAAGCAATATCT+TGG | - | chr4.2:20083700-20083719 | MS.gene90239:CDS | 35.0% | |
| TACTAACATCAGCTTCAAGT+TGG | + | chr4.2:20083741-20083760 | None:intergenic | 35.0% | |
| ! | GTAGTTAGCTCTTCTGAAAA+CGG | - | chr4.2:20083796-20083815 | MS.gene90239:CDS | 35.0% |
| TGATGAAGTGATCGAGTCTT+TGG | - | chr4.2:20083612-20083631 | MS.gene90239:CDS | 40.0% | |
| ! | AAGTGATCGAGTCTTTGGAA+TGG | - | chr4.2:20083617-20083636 | MS.gene90239:CDS | 40.0% |
| ! | TGAATCTCATAGGAGTGTGT+TGG | - | chr4.2:20083654-20083673 | MS.gene90239:CDS | 40.0% |
| AAGCAATATCTTGGGGAGGA+TGG | - | chr4.2:20083709-20083728 | MS.gene90239:CDS | 45.0% | |
| ACTCAAGCAATATCTTGGGG+AGG | - | chr4.2:20083705-20083724 | MS.gene90239:CDS | 45.0% | |
| AGCAATATCTTGGGGAGGAT+GGG | - | chr4.2:20083710-20083729 | MS.gene90239:CDS | 45.0% | |
| TCTCGAGGCATGAATCTCAT+AGG | - | chr4.2:20083644-20083663 | MS.gene90239:CDS | 45.0% | |
| TGTGTTGGAAGAGCAGTTGA+GGG | - | chr4.2:20083669-20083688 | MS.gene90239:CDS | 45.0% | |
| GTGTGTTGGAAGAGCAGTTG+AGG | - | chr4.2:20083668-20083687 | MS.gene90239:CDS | 50.0% | |
| !! | CTTTGGAATGGACGATCTCG+AGG | - | chr4.2:20083629-20083648 | MS.gene90239:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 20083586 | 20083834 | 20083586 | ID=MS.gene90239 |
| chr4.2 | mRNA | 20083586 | 20083834 | 20083586 | ID=MS.gene90239.t1;Parent=MS.gene90239 |
| chr4.2 | exon | 20083586 | 20083834 | 20083586 | ID=MS.gene90239.t1.exon1;Parent=MS.gene90239.t1 |
| chr4.2 | CDS | 20083586 | 20083834 | 20083586 | ID=cds.MS.gene90239.t1;Parent=MS.gene90239.t1 |
| Gene Sequence |
| Protein sequence |
