Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91845.t1 | XP_003599439.1 | 93.3 | 403 | 20 | 1 | 1 | 396 | 1 | 403 | 1.90E-192 | 681.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91845.t1 | Q9ZSL6 | 55.1 | 301 | 126 | 1 | 103 | 394 | 114 | 414 | 2.3e-85 | 317.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91845.t1 | G7J0X0 | 93.3 | 403 | 20 | 1 | 1 | 396 | 1 | 403 | 1.4e-192 | 681.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene91845 | 0.811524 | 6.71E-51 | -1.69E-46 |
| MS.gene049151 | MS.gene91845 | 0.803078 | 4.14E-49 | -1.69E-46 |
| MS.gene049153 | MS.gene91845 | 0.824015 | 1.01E-53 | -1.69E-46 |
| MS.gene049346 | MS.gene91845 | 0.847276 | 1.28E-59 | -1.69E-46 |
| MS.gene049758 | MS.gene91845 | 0.858837 | 6.26E-63 | -1.69E-46 |
| MS.gene049910 | MS.gene91845 | 0.864805 | 9.34E-65 | -1.69E-46 |
| MS.gene049915 | MS.gene91845 | 0.833462 | 5.26E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene91845 | 0.843482 | 1.36E-58 | -1.69E-46 |
| MS.gene050330 | MS.gene91845 | -0.812326 | 4.48E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene91845 | 0.834304 | 3.24E-56 | -1.69E-46 |
| MS.gene050398 | MS.gene91845 | 0.813868 | 2.06E-51 | -1.69E-46 |
| MS.gene050404 | MS.gene91845 | 0.828313 | 9.63E-55 | -1.69E-46 |
| MS.gene050406 | MS.gene91845 | 0.866149 | 3.52E-65 | -1.69E-46 |
| MS.gene050426 | MS.gene91845 | -0.807125 | 5.89E-50 | -1.69E-46 |
| MS.gene050464 | MS.gene91845 | 0.835783 | 1.37E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene91845 | 0.827222 | 1.76E-54 | -1.69E-46 |
| MS.gene050642 | MS.gene91845 | 0.838573 | 2.65E-57 | -1.69E-46 |
| MS.gene051471 | MS.gene91845 | 0.816926 | 4.30E-52 | -1.69E-46 |
| MS.gene051582 | MS.gene91845 | 0.807257 | 5.52E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene91845 | 0.80027 | 1.56E-48 | -1.69E-46 |
| MS.gene051860 | MS.gene91845 | 0.834908 | 2.28E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene91845 | 0.834883 | 2.32E-56 | -1.69E-46 |
| MS.gene052077 | MS.gene91845 | 0.874084 | 8.90E-68 | -1.69E-46 |
| MS.gene052078 | MS.gene91845 | 0.826557 | 2.54E-54 | -1.69E-46 |
| MS.gene052178 | MS.gene91845 | 0.82487 | 6.39E-54 | -1.69E-46 |
| MS.gene052188 | MS.gene91845 | 0.824681 | 7.07E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene91845 | 0.862288 | 5.64E-64 | -1.69E-46 |
| MS.gene052255 | MS.gene91845 | 0.83027 | 3.23E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene91845 | 0.830562 | 2.74E-55 | -1.69E-46 |
| MS.gene052340 | MS.gene91845 | 0.865819 | 4.48E-65 | -1.69E-46 |
| MS.gene053073 | MS.gene91845 | 0.81323 | 2.84E-51 | -1.69E-46 |
| MS.gene053557 | MS.gene91845 | 0.837562 | 4.83E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene91845 | 0.873765 | 1.14E-67 | -1.69E-46 |
| MS.gene053928 | MS.gene91845 | 0.821739 | 3.44E-53 | -1.69E-46 |
| MS.gene054123 | MS.gene91845 | 0.80353 | 3.34E-49 | -1.69E-46 |
| MS.gene05413 | MS.gene91845 | 0.803743 | 3.01E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene91845 | 0.812352 | 4.43E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene91845 | 0.847404 | 1.18E-59 | -1.69E-46 |
| MS.gene054542 | MS.gene91845 | 0.804616 | 1.98E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene91845 | 0.825192 | 5.36E-54 | -1.69E-46 |
| MS.gene054983 | MS.gene91845 | 0.843873 | 1.07E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene91845 | 0.843783 | 1.13E-58 | -1.69E-46 |
| MS.gene055277 | MS.gene91845 | 0.801748 | 7.78E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene91845 | 0.832549 | 8.87E-56 | -1.69E-46 |
| MS.gene055428 | MS.gene91845 | 0.821086 | 4.87E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene91845 | 0.845423 | 4.10E-59 | -1.69E-46 |
| MS.gene055604 | MS.gene91845 | 0.801292 | 9.65E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene91845 | 0.806941 | 6.44E-50 | -1.69E-46 |
| MS.gene056451 | MS.gene91845 | 0.822405 | 2.41E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene91845 | 0.836557 | 8.73E-57 | -1.69E-46 |
| MS.gene056875 | MS.gene91845 | 0.802177 | 6.35E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene91845 | -0.822184 | 2.71E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene91845 | 0.817975 | 2.49E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene91845 | 0.854363 | 1.29E-61 | -1.69E-46 |
| MS.gene057121 | MS.gene91845 | 0.830223 | 3.31E-55 | -1.69E-46 |
| MS.gene057245 | MS.gene91845 | 0.800102 | 1.69E-48 | -1.69E-46 |
| MS.gene057396 | MS.gene91845 | 0.839194 | 1.83E-57 | -1.69E-46 |
| MS.gene057448 | MS.gene91845 | 0.812468 | 4.18E-51 | -1.69E-46 |
| MS.gene057449 | MS.gene91845 | 0.807638 | 4.58E-50 | -1.69E-46 |
| MS.gene057450 | MS.gene91845 | 0.811145 | 8.11E-51 | -1.69E-46 |
| MS.gene057750 | MS.gene91845 | 0.807219 | 5.62E-50 | -1.69E-46 |
| MS.gene057754 | MS.gene91845 | 0.842723 | 2.17E-58 | -1.69E-46 |
| MS.gene058002 | MS.gene91845 | 0.872767 | 2.47E-67 | -1.69E-46 |
| MS.gene05811 | MS.gene91845 | 0.855111 | 7.85E-62 | -1.69E-46 |
| MS.gene058142 | MS.gene91845 | 0.849501 | 3.10E-60 | -1.69E-46 |
| MS.gene058233 | MS.gene91845 | 0.816469 | 5.44E-52 | -1.69E-46 |
| MS.gene058276 | MS.gene91845 | 0.826141 | 3.19E-54 | -1.69E-46 |
| MS.gene058710 | MS.gene91845 | 0.80876 | 2.64E-50 | -1.69E-46 |
| MS.gene058749 | MS.gene91845 | 0.835173 | 1.96E-56 | -1.69E-46 |
| MS.gene058845 | MS.gene91845 | 0.828418 | 9.09E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene91845 | 0.810398 | 1.18E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene91845 | 0.846659 | 1.89E-59 | -1.69E-46 |
| MS.gene059247 | MS.gene91845 | 0.805375 | 1.38E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene91845 | 0.843772 | 1.14E-58 | -1.69E-46 |
| MS.gene059420 | MS.gene91845 | 0.811403 | 7.12E-51 | -1.69E-46 |
| MS.gene059421 | MS.gene91845 | 0.807768 | 4.30E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene91845 | 0.826316 | 2.90E-54 | -1.69E-46 |
| MS.gene059597 | MS.gene91845 | 0.816702 | 4.83E-52 | -1.69E-46 |
| MS.gene059688 | MS.gene91845 | 0.807757 | 4.33E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene91845 | 0.82722 | 1.76E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene91845 | 0.819857 | 9.32E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene91845 | 0.848105 | 7.58E-60 | -1.69E-46 |
| MS.gene059894 | MS.gene91845 | 0.862975 | 3.46E-64 | -1.69E-46 |
| MS.gene059899 | MS.gene91845 | 0.806811 | 6.86E-50 | -1.69E-46 |
| MS.gene06037 | MS.gene91845 | 0.82833 | 9.55E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene91845 | 0.80039 | 1.47E-48 | -1.69E-46 |
| MS.gene060407 | MS.gene91845 | 0.819138 | 1.36E-52 | -1.69E-46 |
| MS.gene060476 | MS.gene91845 | 0.811892 | 5.58E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene91845 | 0.902617 | 8.07E-79 | -1.69E-46 |
| MS.gene061159 | MS.gene91845 | 0.819837 | 9.42E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene052009 | MS.gene91845 | PPI |
| MS.gene032180 | MS.gene91845 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91845.t1 | MTR_3g033420 | 93.300 | 403 | 20 | 1 | 1 | 396 | 1 | 403 | 0.0 | 692 |
| MS.gene91845.t1 | MTR_3g033150 | 94.805 | 154 | 8 | 0 | 243 | 396 | 1 | 154 | 1.79e-92 | 275 |
| MS.gene91845.t1 | MTR_7g101840 | 38.462 | 247 | 152 | 0 | 149 | 395 | 307 | 553 | 6.57e-48 | 171 |
| MS.gene91845.t1 | MTR_3g118290 | 36.709 | 237 | 150 | 0 | 160 | 396 | 335 | 571 | 5.66e-46 | 166 |
| MS.gene91845.t1 | MTR_0172s0030 | 36.420 | 324 | 174 | 8 | 84 | 384 | 176 | 490 | 8.90e-43 | 156 |
| MS.gene91845.t1 | MTR_1g063850 | 32.323 | 297 | 189 | 2 | 111 | 395 | 261 | 557 | 3.64e-42 | 155 |
| MS.gene91845.t1 | MTR_1g007370 | 33.468 | 248 | 158 | 2 | 149 | 395 | 306 | 547 | 7.75e-38 | 144 |
| MS.gene91845.t1 | MTR_3g090900 | 29.070 | 258 | 176 | 4 | 143 | 396 | 260 | 514 | 3.67e-18 | 86.7 |
| MS.gene91845.t1 | MTR_1g007370 | 39.326 | 89 | 54 | 0 | 149 | 237 | 306 | 394 | 4.59e-16 | 79.7 |
| MS.gene91845.t1 | MTR_3g090900 | 29.878 | 164 | 110 | 3 | 143 | 302 | 260 | 422 | 9.69e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91845.t1 | AT5G13730 | 55.150 | 301 | 126 | 1 | 103 | 394 | 114 | 414 | 4.25e-109 | 327 |
| MS.gene91845.t1 | AT2G36990 | 32.907 | 313 | 198 | 2 | 95 | 395 | 231 | 543 | 2.50e-48 | 172 |
| MS.gene91845.t1 | AT1G08540 | 31.988 | 322 | 206 | 2 | 87 | 396 | 249 | 569 | 3.03e-46 | 167 |
| MS.gene91845.t1 | AT1G64860 | 33.728 | 338 | 198 | 7 | 68 | 384 | 156 | 488 | 3.63e-41 | 152 |
| MS.gene91845.t1 | AT3G53920 | 30.847 | 295 | 191 | 3 | 114 | 395 | 275 | 569 | 1.71e-35 | 137 |
| MS.gene91845.t1 | AT1G64860 | 32.595 | 316 | 187 | 7 | 68 | 362 | 156 | 466 | 8.75e-34 | 132 |
| MS.gene91845.t1 | AT5G24120 | 27.982 | 218 | 149 | 4 | 182 | 396 | 305 | 517 | 2.64e-16 | 80.9 |
| MS.gene91845.t1 | AT5G24120 | 27.982 | 218 | 149 | 4 | 182 | 396 | 305 | 517 | 2.64e-16 | 80.9 |
| MS.gene91845.t1 | AT5G24120 | 27.982 | 218 | 149 | 4 | 182 | 396 | 305 | 517 | 2.64e-16 | 80.9 |
| MS.gene91845.t1 | AT3G53920 | 33.088 | 136 | 79 | 2 | 114 | 237 | 275 | 410 | 6.58e-15 | 76.3 |
Find 73 sgRNAs with CRISPR-Local
Find 144 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAAAGTTTGGTACAAATT+TGG | 0.082820 | 3.2:-22314140 | MS.gene91845:CDS |
| GACAACTTACATCCTCTATT+TGG | 0.220456 | 3.2:+22313059 | None:intergenic |
| TCAAAGTTTGGTACAAATTT+GGG | 0.281698 | 3.2:-22314139 | MS.gene91845:CDS |
| ATAGCTCTGTCCAATGAAAT+TGG | 0.288432 | 3.2:+22312240 | None:intergenic |
| TTTGGTACAAATTTGGGTTA+TGG | 0.319268 | 3.2:-22314133 | MS.gene91845:CDS |
| ACAAGGAATGATTAAAGCTT+TGG | 0.341968 | 3.2:-22313012 | MS.gene91845:CDS |
| TATTTATAGGGAGAAAAGTA+TGG | 0.343959 | 3.2:-22312399 | MS.gene91845:intron |
| ACTATTACACCCTCAAAGTT+TGG | 0.347291 | 3.2:-22314151 | MS.gene91845:CDS |
| GCTAGAAATGCTGCAGCTGC+TGG | 0.362443 | 3.2:-22314046 | MS.gene91845:CDS |
| GTTGTTGGGTCTGTGACATC+AGG | 0.384102 | 3.2:-22313914 | MS.gene91845:CDS |
| GGAACCATTGGACTTCTTCA+TGG | 0.384588 | 3.2:-22313098 | MS.gene91845:CDS |
| AATGCTGCAGCTGCTGGAAT+AGG | 0.387273 | 3.2:-22314040 | MS.gene91845:CDS |
| ATATTATACTTTGGACTCAA+TGG | 0.405310 | 3.2:-22311947 | MS.gene91845:CDS |
| AGCTGCATCCACAGCATCAT+TGG | 0.408165 | 3.2:+22314066 | None:intergenic |
| GTCTTTAGAGATGGAAAAGT+TGG | 0.408480 | 3.2:+22314202 | None:intergenic |
| GAGCAGACAACCAATTTCAT+TGG | 0.418328 | 3.2:-22312250 | MS.gene91845:CDS |
| AAGCATTCAAGATCTCATTC+AGG | 0.419494 | 3.2:-22313197 | MS.gene91845:intron |
| CAAATTTGTACCAAACTTTG+AGG | 0.431037 | 3.2:+22314141 | None:intergenic |
| CACCGGTAATCTGACTAATC+TGG | 0.431896 | 3.2:+22312980 | None:intergenic |
| TGAGGCAGAAACATGTTGTT+GGG | 0.432097 | 3.2:-22313928 | MS.gene91845:CDS |
| GAGATTGTAATATTATACTT+TGG | 0.445378 | 3.2:-22311956 | MS.gene91845:CDS |
| GTCCAGATTAGTCAGATTAC+CGG | 0.449365 | 3.2:-22312982 | MS.gene91845:intron |
| CTATCTCCTCAAAAGACAAA+GGG | 0.452640 | 3.2:+22311918 | None:intergenic |
| GAATAGGAATTGATGATATG+AGG | 0.458742 | 3.2:-22314024 | MS.gene91845:CDS |
| GGGACCAGTCGAAATGATTC+CGG | 0.462891 | 3.2:-22312053 | MS.gene91845:CDS |
| TTGAGGCAGAAACATGTTGT+TGG | 0.472004 | 3.2:-22313929 | MS.gene91845:CDS |
| GAAAGTTGTTGAAACTATCA+AGG | 0.474029 | 3.2:-22311895 | MS.gene91845:CDS |
| ATGATATGAGGAGGAAGAGA+AGG | 0.474045 | 3.2:-22314012 | MS.gene91845:CDS |
| GAGAAATTTGATCCAAATAG+AGG | 0.478360 | 3.2:-22313071 | MS.gene91845:CDS |
| GGAATCATTTCGACTGGTCC+CGG | 0.483039 | 3.2:+22312055 | None:intergenic |
| CGAAATGATTCCGGAGAAGA+TGG | 0.490563 | 3.2:-22312044 | MS.gene91845:CDS |
| CAGTATTAAGTTGCTGTGAT+CGG | 0.500229 | 3.2:+22312215 | None:intergenic |
| GAGAGAATCAGACAGATTCA+TGG | 0.502535 | 3.2:-22311872 | MS.gene91845:CDS |
| TTTAACCAGGAAGGAACCAT+TGG | 0.503437 | 3.2:-22313110 | MS.gene91845:intron |
| TGAGCTTTGTTTATGTCTCA+AGG | 0.503531 | 3.2:-22313867 | MS.gene91845:intron |
| AGAGGCAGTTAGTGAAGCAA+GGG | 0.505063 | 3.2:-22312018 | MS.gene91845:CDS |
| AGTTGTCTACATATGTATAC+TGG | 0.508604 | 3.2:-22313043 | MS.gene91845:CDS |
| AAATTTGTACCAAACTTTGA+GGG | 0.511674 | 3.2:+22314142 | None:intergenic |
| TTCTCCGGAATCATTTCGAC+TGG | 0.516388 | 3.2:+22312049 | None:intergenic |
| TGCCTCTCAACCATCTTCTC+CGG | 0.517628 | 3.2:+22312034 | None:intergenic |
| TATAATGCAGGAGATTATAC+CGG | 0.518435 | 3.2:-22312074 | MS.gene91845:intron |
| TAAGCAAAGAATGACAGAAC+AGG | 0.522974 | 3.2:-22313347 | MS.gene91845:CDS |
| TCAATCGCAGCACGTTATCA+AGG | 0.523165 | 3.2:-22313229 | MS.gene91845:CDS |
| AACTTTGAGGGTGTAATAGT+AGG | 0.530053 | 3.2:+22314154 | None:intergenic |
| CTGTAAAACTTGTTTCTGAA+AGG | 0.534187 | 3.2:-22312272 | MS.gene91845:CDS |
| CCTATCTCCTCAAAAGACAA+AGG | 0.538706 | 3.2:+22311917 | None:intergenic |
| TCAGCAATTTCATTATATGT+CGG | 0.554776 | 3.2:+22312318 | None:intergenic |
| GAGAGGCAGTTAGTGAAGCA+AGG | 0.557933 | 3.2:-22312019 | MS.gene91845:CDS |
| GAATGGTGTGTCTTTAGAGA+TGG | 0.559338 | 3.2:+22314193 | None:intergenic |
| TCTTAAGACATTGTTAGCCT+CGG | 0.567970 | 3.2:+22312359 | None:intergenic |
| ATAGGGAGAAAAGTATGGAA+TGG | 0.569556 | 3.2:-22312394 | MS.gene91845:intron |
| ATGTCTTAAGACGAAGACTA+AGG | 0.570194 | 3.2:-22312347 | MS.gene91845:CDS |
| AAAGTTGTTGAAACTATCAA+GGG | 0.579121 | 3.2:-22311894 | MS.gene91845:CDS |
| TTGTTGGGTCTGTGACATCA+GGG | 0.583758 | 3.2:-22313913 | MS.gene91845:CDS |
| TGCTCCATGAAGAAGTCCAA+TGG | 0.596543 | 3.2:+22313094 | None:intergenic |
| TGGAAGTGATGTTAGAGACA+TGG | 0.602214 | 3.2:+22314222 | None:intergenic |
| TGGTGATGCATTAGTCATTG+TGG | 0.605317 | 3.2:-22314113 | MS.gene91845:CDS |
| GCAGCACGTTATCAAGGCAA+AGG | 0.605612 | 3.2:-22313223 | MS.gene91845:CDS |
| TAGGAATTGATGATATGAGG+AGG | 0.618372 | 3.2:-22314021 | MS.gene91845:CDS |
| TCACAGCAACTTAATACTGA+AGG | 0.619257 | 3.2:-22312211 | MS.gene91845:intron |
| CAAAGAATGACAGAACAGGA+AGG | 0.619815 | 3.2:-22313343 | MS.gene91845:CDS |
| TGTCTACATATGTATACTGG+TGG | 0.621418 | 3.2:-22313040 | MS.gene91845:CDS |
| TTCCGGAGAAGATGGTTGAG+AGG | 0.622689 | 3.2:-22312036 | MS.gene91845:CDS |
| GTATACTGGTGGATCAAACA+AGG | 0.626050 | 3.2:-22313029 | MS.gene91845:CDS |
| AAAGAATGACAGAACAGGAA+GGG | 0.627713 | 3.2:-22313342 | MS.gene91845:CDS |
| ATATTCAGAACAATTTCTTG+AGG | 0.629406 | 3.2:-22313946 | MS.gene91845:CDS |
| ATAATGCAGGAGATTATACC+GGG | 0.633370 | 3.2:-22312073 | MS.gene91845:intron |
| TGCATTAGCCAATGATGCTG+TGG | 0.639996 | 3.2:-22314074 | MS.gene91845:CDS |
| GACATATAATGAAATTGCTG+AGG | 0.644481 | 3.2:-22312316 | MS.gene91845:CDS |
| GAGGCAGTTAGTGAAGCAAG+GGG | 0.667329 | 3.2:-22312017 | MS.gene91845:CDS |
| TATCTCCTCAAAAGACAAAG+GGG | 0.686203 | 3.2:+22311919 | None:intergenic |
| TCTTAAGACGAAGACTAAGG+CGG | 0.687494 | 3.2:-22312344 | MS.gene91845:CDS |
| AGATTAGCTCGTGATTATCG+CGG | 0.718368 | 3.2:-22313262 | MS.gene91845:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATAGCTTTTTTTTTTTTT+GGG | - | chr3.2:22313081-22313100 | MS.gene91845:CDS | 10.0% |
| !!! | TTTATAGCTTTTTTTTTTTT+TGG | - | chr3.2:22313080-22313099 | MS.gene91845:CDS | 10.0% |
| !! | GATATATAAGTAGATAAATT+TGG | + | chr3.2:22312543-22312562 | None:intergenic | 15.0% |
| !! | GTTAAAAATAATATGAAAAG+TGG | - | chr3.2:22312386-22312405 | MS.gene91845:CDS | 15.0% |
| !!! | TATAGCTTTTTTTTTTTTTG+GGG | - | chr3.2:22313082-22313101 | MS.gene91845:CDS | 15.0% |
| !!! | TATTAATGTCTACAATTTTA+GGG | + | chr3.2:22312280-22312299 | None:intergenic | 15.0% |
| !! | AGAAAAAGAAGGAAAAATTT+GGG | - | chr3.2:22312039-22312058 | MS.gene91845:CDS | 20.0% |
| !! | GAGATTGTAATATTATACTT+TGG | - | chr3.2:22314072-22314091 | MS.gene91845:CDS | 20.0% |
| !! | TATATGTTAATTATCATCCA+CGG | + | chr3.2:22312445-22312464 | None:intergenic | 20.0% |
| !! | TGGATGATAATTAACATATA+TGG | - | chr3.2:22312445-22312464 | MS.gene91845:intron | 20.0% |
| !!! | AGTAACCAATTTTATTTTTC+CGG | + | chr3.2:22313403-22313422 | None:intergenic | 20.0% |
| !!! | ATAGCTTTTTTTTTTTTTGG+GGG | - | chr3.2:22313083-22313102 | MS.gene91845:CDS | 20.0% |
| !!! | ATTTGTTAAAGTTTCTCATT+AGG | - | chr3.2:22312883-22312902 | MS.gene91845:intron | 20.0% |
| !!! | ATTTTACAACTATTCTAAGA+AGG | - | chr3.2:22313153-22313172 | MS.gene91845:intron | 20.0% |
| !!! | ATTTTTACTGATGTATGTTT+CGG | + | chr3.2:22313369-22313388 | None:intergenic | 20.0% |
| !!! | CTATTAATGTCTACAATTTT+AGG | + | chr3.2:22312281-22312300 | None:intergenic | 20.0% |
| !!! | GACAATTTCATTTGTATTTT+AGG | - | chr3.2:22313843-22313862 | MS.gene91845:intron | 20.0% |
| !!! | TGGTTAGTTTGTTATTTATA+GGG | - | chr3.2:22313617-22313636 | MS.gene91845:intron | 20.0% |
| !!! | TTGGTTAGTTTGTTATTTAT+AGG | - | chr3.2:22313616-22313635 | MS.gene91845:intron | 20.0% |
| ! | AAAAAAAACCATGTTACTCA+AGG | + | chr3.2:22313274-22313293 | None:intergenic | 25.0% |
| ! | AAAGTTGTTGAAACTATCAA+GGG | - | chr3.2:22314134-22314153 | MS.gene91845:CDS | 25.0% |
| ! | AAATTTGTACCAAACTTTGA+GGG | + | chr3.2:22311889-22311908 | None:intergenic | 25.0% |
| ! | AACTTCGAAATCAATTTCAA+TGG | + | chr3.2:22312482-22312501 | None:intergenic | 25.0% |
| ! | ATATTATACTTTGGACTCAA+TGG | - | chr3.2:22314081-22314100 | MS.gene91845:CDS | 25.0% |
| ! | ATATTCAGAACAATTTCTTG+AGG | - | chr3.2:22312082-22312101 | MS.gene91845:intron | 25.0% |
| ! | GACTGTTTATTATGAATGTT+TGG | - | chr3.2:22313597-22313616 | MS.gene91845:intron | 25.0% |
| ! | GAGAAAAAGAAGGAAAAATT+TGG | - | chr3.2:22312038-22312057 | MS.gene91845:CDS | 25.0% |
| ! | GTAAAAATTGATATACCAAC+CGG | - | chr3.2:22313381-22313400 | MS.gene91845:intron | 25.0% |
| ! | TACTATGCAATACTTCTATA+TGG | - | chr3.2:22313548-22313567 | MS.gene91845:intron | 25.0% |
| ! | TATTTATAGGGAGAAAAGTA+TGG | - | chr3.2:22313629-22313648 | MS.gene91845:intron | 25.0% |
| ! | TCAAAGTTTGGTACAAATTT+GGG | - | chr3.2:22311889-22311908 | MS.gene91845:CDS | 25.0% |
| ! | TCAGCAATTTCATTATATGT+CGG | + | chr3.2:22313713-22313732 | None:intergenic | 25.0% |
| ! | TTTAATTCATTTAACCAGGA+AGG | - | chr3.2:22312909-22312928 | MS.gene91845:intron | 25.0% |
| !! | TTGATTAGACGAATTTTGTT+AGG | - | chr3.2:22313299-22313318 | MS.gene91845:CDS | 25.0% |
| !!! | GCATTTTAATTCATTTAACC+AGG | - | chr3.2:22312905-22312924 | MS.gene91845:intron | 25.0% |
| !!! | TACAATTTTAGGGACTAATA+TGG | + | chr3.2:22312270-22312289 | None:intergenic | 25.0% |
| !!! | TTTTTTTTTTTTTGGGGGAT+GGG | - | chr3.2:22313088-22313107 | MS.gene91845:CDS | 25.0% |
| ACAAGGAATGATTAAAGCTT+TGG | - | chr3.2:22313016-22313035 | MS.gene91845:CDS | 30.0% | |
| ACCAACCGGAAAAATAAAAT+TGG | - | chr3.2:22313395-22313414 | MS.gene91845:intron | 30.0% | |
| AGTTGTCTACATATGTATAC+TGG | - | chr3.2:22312985-22313004 | MS.gene91845:CDS | 30.0% | |
| ATTCACATTCGATTACTAAC+AGG | - | chr3.2:22312607-22312626 | MS.gene91845:intron | 30.0% | |
| CAAATTTGTACCAAACTTTG+AGG | + | chr3.2:22311890-22311909 | None:intergenic | 30.0% | |
| CTCAAAGTTTGGTACAAATT+TGG | - | chr3.2:22311888-22311907 | MS.gene91845:CDS | 30.0% | |
| GAAAGTTGTTGAAACTATCA+AGG | - | chr3.2:22314133-22314152 | MS.gene91845:CDS | 30.0% | |
| GAATTTAAATCATATCCAGC+AGG | + | chr3.2:22312591-22312610 | None:intergenic | 30.0% | |
| GACATATAATGAAATTGCTG+AGG | - | chr3.2:22313712-22313731 | MS.gene91845:intron | 30.0% | |
| GAGAAATTTGATCCAAATAG+AGG | - | chr3.2:22312957-22312976 | MS.gene91845:intron | 30.0% | |
| TATAATGCAGGAGATTATAC+CGG | - | chr3.2:22313954-22313973 | MS.gene91845:CDS | 30.0% | |
| TGTTTACAACGATATAATGC+AGG | - | chr3.2:22313942-22313961 | MS.gene91845:CDS | 30.0% | |
| ! | AAGGAAAAATTTGGGATGTA+TGG | - | chr3.2:22312047-22312066 | MS.gene91845:CDS | 30.0% |
| ! | CACTATTTTCATAGTCTCTT+TGG | - | chr3.2:22313485-22313504 | MS.gene91845:intron | 30.0% |
| ! | GAATAGGAATTGATGATATG+AGG | - | chr3.2:22312004-22312023 | MS.gene91845:CDS | 30.0% |
| ! | GTGAATTTTGTTGAAGTGAA+TGG | + | chr3.2:22311855-22311874 | None:intergenic | 30.0% |
| ! | TAGACGAATTTTGTTAGGTA+TGG | - | chr3.2:22313304-22313323 | MS.gene91845:CDS | 30.0% |
| ! | TATAATGCAAATTTTCCTGC+TGG | - | chr3.2:22312573-22312592 | MS.gene91845:intron | 30.0% |
| ! | TTTGGTACAAATTTGGGTTA+TGG | - | chr3.2:22311895-22311914 | MS.gene91845:CDS | 30.0% |
| !! | AATTTTAGGGACTAATATGG+TGG | + | chr3.2:22312267-22312286 | None:intergenic | 30.0% |
| !! | CTGTAAAACTTGTTTCTGAA+AGG | - | chr3.2:22313756-22313775 | MS.gene91845:intron | 30.0% |
| !! | GATTTTAACCTTGAGTAACA+TGG | - | chr3.2:22313263-22313282 | MS.gene91845:CDS | 30.0% |
| !!! | ACCAATTTTATTTTTCCGGT+TGG | + | chr3.2:22313399-22313418 | None:intergenic | 30.0% |
| !!! | CTTTTTTTTTTTTTGGGGGA+TGG | - | chr3.2:22313087-22313106 | MS.gene91845:CDS | 30.0% |
| !!! | TTTTTTTTTTTTGGGGGATG+GGG | - | chr3.2:22313089-22313108 | MS.gene91845:CDS | 30.0% |
| AAAGAATGACAGAACAGGAA+GGG | - | chr3.2:22312686-22312705 | MS.gene91845:intron | 35.0% | |
| AAGCATTCAAGATCTCATTC+AGG | - | chr3.2:22312831-22312850 | MS.gene91845:intron | 35.0% | |
| ACTATTACACCCTCAAAGTT+TGG | - | chr3.2:22311877-22311896 | MS.gene91845:CDS | 35.0% | |
| ATAATGCAGGAGATTATACC+GGG | - | chr3.2:22313955-22313974 | MS.gene91845:CDS | 35.0% | |
| ATGTCTTAAGACGAAGACTA+AGG | - | chr3.2:22313681-22313700 | MS.gene91845:intron | 35.0% | |
| CTATCTCCTCAAAAGACAAA+GGG | + | chr3.2:22314113-22314132 | None:intergenic | 35.0% | |
| GACAACTTACATCCTCTATT+TGG | + | chr3.2:22312972-22312991 | None:intergenic | 35.0% | |
| TAAGCAAAGAATGACAGAAC+AGG | - | chr3.2:22312681-22312700 | MS.gene91845:intron | 35.0% | |
| TATCTCCTCAAAAGACAAAG+GGG | + | chr3.2:22314112-22314131 | None:intergenic | 35.0% | |
| TCACAGCAACTTAATACTGA+AGG | - | chr3.2:22313817-22313836 | MS.gene91845:intron | 35.0% | |
| TGTCTACATATGTATACTGG+TGG | - | chr3.2:22312988-22313007 | MS.gene91845:CDS | 35.0% | |
| ! | AACTTTGAGGGTGTAATAGT+AGG | + | chr3.2:22311877-22311896 | None:intergenic | 35.0% |
| ! | ATAGCTCTGTCCAATGAAAT+TGG | + | chr3.2:22313791-22313810 | None:intergenic | 35.0% |
| ! | ATAGGGAGAAAAGTATGGAA+TGG | - | chr3.2:22313634-22313653 | MS.gene91845:intron | 35.0% |
| ! | CAGTATTAAGTTGCTGTGAT+CGG | + | chr3.2:22313816-22313835 | None:intergenic | 35.0% |
| ! | GAAAAATTTGGGATGTATGG+TGG | - | chr3.2:22312050-22312069 | MS.gene91845:CDS | 35.0% |
| ! | GTCTTTAGAGATGGAAAAGT+TGG | + | chr3.2:22311829-22311848 | None:intergenic | 35.0% |
| ! | TAGGAATTGATGATATGAGG+AGG | - | chr3.2:22312007-22312026 | MS.gene91845:CDS | 35.0% |
| ! | TCTTAAGACATTGTTAGCCT+CGG | + | chr3.2:22313672-22313691 | None:intergenic | 35.0% |
| !! | GCTATAAAAAAGTGTCTCAC+CGG | + | chr3.2:22313068-22313087 | None:intergenic | 35.0% |
| !! | TGAGCTTTGTTTATGTCTCA+AGG | - | chr3.2:22312161-22312180 | MS.gene91845:intron | 35.0% |
| !! | TGTGTTGATTAACAACAGGA+GGG | - | chr3.2:22312640-22312659 | MS.gene91845:intron | 35.0% |
| !!! | TTTTTTTTTTTGGGGGATGG+GGG | - | chr3.2:22313090-22313109 | MS.gene91845:CDS | 35.0% |
| AATTTGAACCGTGTCTCTTG+AGG | - | chr3.2:22313177-22313196 | MS.gene91845:intron | 40.0% | |
| ATGATATGAGGAGGAAGAGA+AGG | - | chr3.2:22312016-22312035 | MS.gene91845:CDS | 40.0% | |
| CAAAGAATGACAGAACAGGA+AGG | - | chr3.2:22312685-22312704 | MS.gene91845:intron | 40.0% | |
| CCTATCTCCTCAAAAGACAA+AGG | + | chr3.2:22314114-22314133 | None:intergenic | 40.0% | |
| GAGAGAATCAGACAGATTCA+TGG | - | chr3.2:22314156-22314175 | MS.gene91845:CDS | 40.0% | |
| GAGCAGACAACCAATTTCAT+TGG | - | chr3.2:22313778-22313797 | MS.gene91845:intron | 40.0% | |
| GGAAGAGAAGGAGAAAAAGA+AGG | - | chr3.2:22312028-22312047 | MS.gene91845:CDS | 40.0% | |
| TCTTAAGACGAAGACTAAGG+CGG | - | chr3.2:22313684-22313703 | MS.gene91845:intron | 40.0% | |
| TGAGGCAGAAACATGTTGTT+GGG | - | chr3.2:22312100-22312119 | MS.gene91845:intron | 40.0% | |
| TTGAGGCAGAAACATGTTGT+TGG | - | chr3.2:22312099-22312118 | MS.gene91845:intron | 40.0% | |
| TTTAACCAGGAAGGAACCAT+TGG | - | chr3.2:22312918-22312937 | MS.gene91845:intron | 40.0% | |
| TTTGTTAGGTATGGTGCATG+TGG | - | chr3.2:22313313-22313332 | MS.gene91845:CDS | 40.0% | |
| ! | AGATTAGCTCGTGATTATCG+CGG | - | chr3.2:22312766-22312785 | MS.gene91845:intron | 40.0% |
| ! | GAATGGTGTGTCTTTAGAGA+TGG | + | chr3.2:22311838-22311857 | None:intergenic | 40.0% |
| ! | GTATACTGGTGGATCAAACA+AGG | - | chr3.2:22312999-22313018 | MS.gene91845:CDS | 40.0% |
| ! | GTCCAGATTAGTCAGATTAC+CGG | - | chr3.2:22313046-22313065 | MS.gene91845:CDS | 40.0% |
| ! | TCGTCGTTAATTTTGTTCCG+TGG | - | chr3.2:22312425-22312444 | MS.gene91845:intron | 40.0% |
| ! | TGGTGATGCATTAGTCATTG+TGG | - | chr3.2:22311915-22311934 | MS.gene91845:CDS | 40.0% |
| ! | TGTTGATTAACAACAGGAGG+GGG | - | chr3.2:22312642-22312661 | MS.gene91845:intron | 40.0% |
| !! | CCTTTGTCTTTTGAGGAGAT+AGG | - | chr3.2:22314111-22314130 | MS.gene91845:CDS | 40.0% |
| !! | CGTGTGTGTTGATTAACAAC+AGG | - | chr3.2:22312636-22312655 | MS.gene91845:intron | 40.0% |
| !! | GTGTGTTGATTAACAACAGG+AGG | - | chr3.2:22312639-22312658 | MS.gene91845:intron | 40.0% |
| !! | GTGTTGATTAACAACAGGAG+GGG | - | chr3.2:22312641-22312660 | MS.gene91845:intron | 40.0% |
| !!! | TTTTTTTTTTGGGGGATGGG+GGG | - | chr3.2:22313091-22313110 | MS.gene91845:CDS | 40.0% |
| AATGGTTGCAAAAATCGCCG+AGG | - | chr3.2:22313652-22313671 | MS.gene91845:intron | 45.0% | |
| AGAGGCAGTTAGTGAAGCAA+GGG | - | chr3.2:22314010-22314029 | MS.gene91845:CDS | 45.0% | |
| CACCGGTAATCTGACTAATC+TGG | + | chr3.2:22313051-22313070 | None:intergenic | 45.0% | |
| CCTTCTAACCTCAAGAGACA+CGG | + | chr3.2:22313188-22313207 | None:intergenic | 45.0% | |
| CGAAATGATTCCGGAGAAGA+TGG | - | chr3.2:22313984-22314003 | MS.gene91845:CDS | 45.0% | |
| GGAACCATTGGACTTCTTCA+TGG | - | chr3.2:22312930-22312949 | MS.gene91845:intron | 45.0% | |
| TCAATCGCAGCACGTTATCA+AGG | - | chr3.2:22312799-22312818 | MS.gene91845:intron | 45.0% | |
| TGCATTAGCCAATGATGCTG+TGG | - | chr3.2:22311954-22311973 | MS.gene91845:CDS | 45.0% | |
| TGCTCCATGAAGAAGTCCAA+TGG | + | chr3.2:22312937-22312956 | None:intergenic | 45.0% | |
| TTCTCCGGAATCATTTCGAC+TGG | + | chr3.2:22313982-22314001 | None:intergenic | 45.0% | |
| TTGTTGGGTCTGTGACATCA+GGG | - | chr3.2:22312115-22312134 | MS.gene91845:intron | 45.0% | |
| ! | AGAGACCCCTTTGTCTTTTG+AGG | - | chr3.2:22314104-22314123 | MS.gene91845:CDS | 45.0% |
| !!! | TTTTTTTTTGGGGGATGGGG+GGG | - | chr3.2:22313092-22313111 | MS.gene91845:CDS | 45.0% |
| AATGCTGCAGCTGCTGGAAT+AGG | - | chr3.2:22311988-22312007 | MS.gene91845:CDS | 50.0% | |
| AGCTGCATCCACAGCATCAT+TGG | + | chr3.2:22311965-22311984 | None:intergenic | 50.0% | |
| ATTAACAACAGGAGGGGGCA+AGG | - | chr3.2:22312647-22312666 | MS.gene91845:intron | 50.0% | |
| CCGTGTCTCTTGAGGTTAGA+AGG | - | chr3.2:22313185-22313204 | MS.gene91845:intron | 50.0% | |
| GAAGTCCAATGGTTCCTTCC+TGG | + | chr3.2:22312926-22312945 | None:intergenic | 50.0% | |
| GAGAGGCAGTTAGTGAAGCA+AGG | - | chr3.2:22314009-22314028 | MS.gene91845:CDS | 50.0% | |
| GAGGCAGTTAGTGAAGCAAG+GGG | - | chr3.2:22314011-22314030 | MS.gene91845:CDS | 50.0% | |
| GCAGCACGTTATCAAGGCAA+AGG | - | chr3.2:22312805-22312824 | MS.gene91845:intron | 50.0% | |
| GGGACCAGTCGAAATGATTC+CGG | - | chr3.2:22313975-22313994 | MS.gene91845:CDS | 50.0% | |
| GTTAGGTATGGTGCATGTGG+AGG | - | chr3.2:22313316-22313335 | MS.gene91845:CDS | 50.0% | |
| GTTGTTGGGTCTGTGACATC+AGG | - | chr3.2:22312114-22312133 | MS.gene91845:intron | 50.0% | |
| TGCCTCTCAACCATCTTCTC+CGG | + | chr3.2:22313997-22314016 | None:intergenic | 50.0% | |
| TTCCGGAGAAGATGGTTGAG+AGG | - | chr3.2:22313992-22314011 | MS.gene91845:CDS | 50.0% | |
| ! | GGAATCATTTCGACTGGTCC+CGG | + | chr3.2:22313976-22313995 | None:intergenic | 50.0% |
| !!! | TTTTTTTTGGGGGATGGGGG+GGG | - | chr3.2:22313093-22313112 | MS.gene91845:CDS | 50.0% |
| GCTAGAAATGCTGCAGCTGC+TGG | - | chr3.2:22311982-22312001 | MS.gene91845:CDS | 55.0% | |
| !!! | TTTTTTTGGGGGATGGGGGG+GGG | - | chr3.2:22313094-22313113 | MS.gene91845:CDS | 55.0% |
| !!! | TTTTTTGGGGGATGGGGGGG+GGG | - | chr3.2:22313095-22313114 | MS.gene91845:CDS | 60.0% |
| !! | TTTTTGGGGGATGGGGGGGG+GGG | - | chr3.2:22313096-22313115 | MS.gene91845:CDS | 65.0% |
| !! | TTTTGGGGGATGGGGGGGGG+GGG | - | chr3.2:22313097-22313116 | MS.gene91845:CDS | 70.0% |
| TTTGGGGGATGGGGGGGGGG+GGG | - | chr3.2:22313098-22313117 | MS.gene91845:CDS | 75.0% | |
| ! | TTGGGGGATGGGGGGGGGGG+GGG | - | chr3.2:22313099-22313118 | MS.gene91845:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 22311808 | 22314242 | 22311808 | ID=MS.gene91845 |
| chr3.2 | mRNA | 22311808 | 22314242 | 22311808 | ID=MS.gene91845.t1;Parent=MS.gene91845 |
| chr3.2 | exon | 22313868 | 22314242 | 22313868 | ID=MS.gene91845.t1.exon1;Parent=MS.gene91845.t1 |
| chr3.2 | CDS | 22313868 | 22314242 | 22313868 | ID=cds.MS.gene91845.t1;Parent=MS.gene91845.t1 |
| chr3.2 | exon | 22313198 | 22313392 | 22313198 | ID=MS.gene91845.t1.exon2;Parent=MS.gene91845.t1 |
| chr3.2 | CDS | 22313198 | 22313392 | 22313198 | ID=cds.MS.gene91845.t1;Parent=MS.gene91845.t1 |
| chr3.2 | exon | 22312983 | 22313123 | 22312983 | ID=MS.gene91845.t1.exon3;Parent=MS.gene91845.t1 |
| chr3.2 | CDS | 22312983 | 22313123 | 22312983 | ID=cds.MS.gene91845.t1;Parent=MS.gene91845.t1 |
| chr3.2 | exon | 22312212 | 22312412 | 22312212 | ID=MS.gene91845.t1.exon4;Parent=MS.gene91845.t1 |
| chr3.2 | CDS | 22312212 | 22312412 | 22312212 | ID=cds.MS.gene91845.t1;Parent=MS.gene91845.t1 |
| chr3.2 | exon | 22311808 | 22312086 | 22311808 | ID=MS.gene91845.t1.exon5;Parent=MS.gene91845.t1 |
| chr3.2 | CDS | 22311808 | 22312086 | 22311808 | ID=cds.MS.gene91845.t1;Parent=MS.gene91845.t1 |
| Gene Sequence |
| Protein sequence |
