AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene93767


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93767.t1 MTR_8g107010 98.047 256 5 0 1 256 1 256 0.0 509
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene93767.t1 AT1G18170 70.430 186 51 2 75 256 62 247 1.15e-95 281
MS.gene93767.t1 AT1G73655 50.000 252 100 4 1 251 1 227 1.50e-79 239
MS.gene93767.t1 AT3G10060 29.379 177 101 5 87 255 60 220 7.03e-13 66.6

Find 80 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGAATGGTGCTGATTT+AGG 0.153499 4.2:-374356 MS.gene93767:CDS
GATGAAACAACTGGTTTAAT+AGG 0.212922 4.2:+375012 None:intergenic
CTAAACATGGCCACTATCTT+TGG 0.258527 4.2:-375210 None:intergenic
GAGAGAATGGTGCTGATTTA+GGG 0.288158 4.2:-374355 MS.gene93767:CDS
AGTTATTGTGCCTCCTGAAT+TGG 0.295641 4.2:-374384 MS.gene93767:CDS
TTTGGTTGTGATTGATATAA+TGG 0.298752 4.2:-374561 MS.gene93767:CDS
TTGACCAGACGGTTTGGCCT+TGG 0.354356 4.2:-374952 MS.gene93767:CDS
TTCTCTCTAAATCCCAATTC+AGG 0.369157 4.2:+374371 None:intergenic
GAAACCATTGGCTCTAGTTA+TGG 0.370613 4.2:-374486 MS.gene93767:CDS
AAACCATTGGCTCTAGTTAT+GGG 0.372920 4.2:-374485 MS.gene93767:CDS
ATAATTAACCTTATGCCATT+AGG 0.374600 4.2:+374702 None:intergenic
TCACACACACCCTTACTATA+TGG 0.378280 4.2:+374455 None:intergenic
GTGATGACTGGTTCTGGTAT+GGG 0.394646 4.2:+375164 None:intergenic
GCCTTGGAGCTGGTCTTGCA+TGG 0.396282 4.2:-374936 MS.gene93767:CDS
ACCAGTTGTTTCATCAACTA+AGG 0.400326 4.2:-375004 MS.gene93767:CDS
GTTATTGTGCCTCCTGAATT+GGG 0.401025 4.2:-374383 MS.gene93767:CDS
TGTGATGACTGGTTCTGGTA+TGG 0.408947 4.2:+375163 None:intergenic
GTTGTTGACTGTGGAGATAA+TGG 0.413539 4.2:+375090 None:intergenic
TATGCCAAGGCGAGGTGATT+TGG 0.421986 4.2:-374579 MS.gene93767:CDS
CCTTGGAGCTGGTCTTGCAT+GGG 0.424931 4.2:-374935 MS.gene93767:CDS
TTGGTTGTGATTGATATAAT+GGG 0.427668 4.2:-374560 MS.gene93767:CDS
GAATGTTGAGGAGGAGAAAG+AGG 0.427854 4.2:-374733 MS.gene93767:CDS
ACTTCCTTGACCAGACGGTT+TGG 0.433297 4.2:-374958 MS.gene93767:CDS
AGACGGTTTGGCCTTGGAGC+TGG 0.438403 4.2:-374946 MS.gene93767:CDS
AGGAATCTGTTGCTGTGCTT+TGG 0.442859 4.2:+375032 None:intergenic
TGATGACTGGTTCTGGTATG+GGG 0.448826 4.2:+375165 None:intergenic
ACCTTAGTTGATGAAACAAC+TGG 0.450082 4.2:+375003 None:intergenic
AGTGATGTGATGACTGGTTC+TGG 0.451572 4.2:+375158 None:intergenic
GGGGTCAAGGCCATATAGTA+AGG 0.452971 4.2:-374465 MS.gene93767:CDS
AGCTCCAAGGCCAAACCGTC+TGG 0.454662 4.2:+374948 None:intergenic
GGAGCTGGTCTTGCATGGGT+TGG 0.455263 4.2:-374931 MS.gene93767:CDS
AGGTACTATGAATTGAAAAT+CGG 0.465928 4.2:-374611 MS.gene93767:CDS
AAAGAGGTGATTCTGCCTAA+TGG 0.469758 4.2:-374717 MS.gene93767:CDS
GTGTTTGTGAACACATTTGA+AGG 0.494203 4.2:-374515 MS.gene93767:CDS
CACAACAAGAATCTAATACA+AGG 0.494667 4.2:-374855 MS.gene93767:intron
GGTTGCGGTTGTTGTGGTGG+AGG 0.494679 4.2:+375120 None:intergenic
GAATTGGGATTTAGAGAGAA+TGG 0.496442 4.2:-374368 MS.gene93767:CDS
AACACTTGAATACGTTGTAC+AGG 0.502708 4.2:-374306 MS.gene93767:CDS
GAGGGTTGCGGTTGTTGTGG+TGG 0.510547 4.2:+375117 None:intergenic
AAACCGTCTGGTCAAGGAAG+TGG 0.514843 4.2:+374960 None:intergenic
CCTGAGGGTTGCGGTTGTTG+TGG 0.518773 4.2:+375114 None:intergenic
TGATTCTGCCTAATGGCATA+AGG 0.521446 4.2:-374710 MS.gene93767:intron
TTCAAATTTGTTGTTGACTG+TGG 0.526248 4.2:+375081 None:intergenic
AATGGTGCTGATTTAGGGTC+TGG 0.527304 4.2:-374350 MS.gene93767:CDS
ATGGGAAAGGTTGAAAGCAC+AGG 0.529021 4.2:-374542 MS.gene93767:CDS
TGATGAGGAACCAAAGATAG+TGG 0.529530 4.2:+375200 None:intergenic
CCACAACAACCGCAACCCTC+AGG 0.533605 4.2:-375114 MS.gene93767:CDS
CTATGAAGGCAGGAGGGAAG+AGG 0.543711 4.2:-374409 MS.gene93767:CDS
TATAGTAAGGGTGTGTGTGA+AGG 0.554244 4.2:-374452 MS.gene93767:CDS
ATAGTAAGGGTGTGTGTGAA+GGG 0.556262 4.2:-374451 MS.gene93767:CDS
GAATCATCAAGCAGGTGCAA+TGG 0.557467 4.2:+374271 None:intergenic
TTGTATTCATAGGAATGTTG+AGG 0.558846 4.2:-374745 MS.gene93767:intron
TTGCCACTTCCTTGACCAGA+CGG 0.563085 4.2:-374963 MS.gene93767:CDS
TGGCTCTAGTTATGGGGTCA+AGG 0.567583 4.2:-374478 MS.gene93767:CDS
TGAAGGAGACAAGAAACCAT+TGG 0.568456 4.2:-374498 MS.gene93767:CDS
CTAAGGTTGATTCCACAGAC+TGG 0.569193 4.2:-374987 MS.gene93767:CDS
TTTAATCAGAATCATCAAGC+AGG 0.570592 4.2:+374263 None:intergenic
AAGGCCAAACCGTCTGGTCA+AGG 0.575540 4.2:+374954 None:intergenic
TACTATGAATTGAAAATCGG+TGG 0.577492 4.2:-374608 MS.gene93767:CDS
GACCCCATAACTAGAGCCAA+TGG 0.581524 4.2:+374482 None:intergenic
GATAATGGACCTGAGGGTTG+CGG 0.588310 4.2:+375105 None:intergenic
TATTCATAGGAATGTTGAGG+AGG 0.588540 4.2:-374742 MS.gene93767:intron
TGTGATTGATATAATGGGAA+AGG 0.597131 4.2:-374555 MS.gene93767:CDS
GATGACTGGTTCTGGTATGG+GGG 0.597654 4.2:+375166 None:intergenic
ACAACCAAATCACCTCGCCT+TGG 0.600709 4.2:+374575 None:intergenic
TATTCAAGTGTTGCAAGTGG+AGG 0.610335 4.2:+374317 None:intergenic
CCCATGCAAGACCAGCTCCA+AGG 0.610919 4.2:+374935 None:intergenic
GGAAGTGGCAATCCAGTCTG+TGG 0.620097 4.2:+374975 None:intergenic
GGGTCAAGGCCATATAGTAA+GGG 0.620171 4.2:-374464 MS.gene93767:CDS
TCGGTGGTGGTGATATGCCA+AGG 0.624769 4.2:-374592 MS.gene93767:CDS
TGAAGAAGTGATGTGATGAC+TGG 0.628381 4.2:+375152 None:intergenic
AACCATTGGCTCTAGTTATG+GGG 0.638329 4.2:-374484 MS.gene93767:CDS
ACGTATTCAAGTGTTGCAAG+TGG 0.645589 4.2:+374314 None:intergenic
TATGAATTGAAAATCGGTGG+TGG 0.648406 4.2:-374605 MS.gene93767:CDS
CTGTGGAGATAATGGACCTG+AGG 0.664299 4.2:+375098 None:intergenic
GGGGAGTAAGAAAATTGATG+AGG 0.664361 4.2:+375185 None:intergenic
TCTCTAAATCCCAATTCAGG+AGG 0.664404 4.2:+374374 None:intergenic
TGCTGATTTAGGGTCTGGTG+TGG 0.665665 4.2:-374345 MS.gene93767:CDS
TGTGGAGATAATGGACCTGA+GGG 0.682825 4.2:+375099 None:intergenic
GGTGGTGATATGCCAAGGCG+AGG 0.718124 4.2:-374587 MS.gene93767:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATCAAAATCATATATGAA+TGG - chr4.2:374687-374706 MS.gene93767:intron 15.0%
!!! AATGATATTTTTGTATTCAT+AGG - chr4.2:374726-374745 MS.gene93767:CDS 15.0%
!! ATATGTACTAAAAACTATGA+AGG - chr4.2:375058-375077 MS.gene93767:CDS 20.0%
! AGGTACTATGAATTGAAAAT+CGG - chr4.2:374870-374889 MS.gene93767:CDS 25.0%
! ATAATTAACCTTATGCCATT+AGG + chr4.2:374782-374801 None:intergenic 25.0%
! TCTCAGAACAAATAAAAACT+CGG - chr4.2:374593-374612 MS.gene93767:CDS 25.0%
! TTGGTTGTGATTGATATAAT+GGG - chr4.2:374921-374940 MS.gene93767:CDS 25.0%
! TTTGGTTGTGATTGATATAA+TGG - chr4.2:374920-374939 MS.gene93767:CDS 25.0%
CACAACAAGAATCTAATACA+AGG - chr4.2:374626-374645 MS.gene93767:intron 30.0%
TACTATGAATTGAAAATCGG+TGG - chr4.2:374873-374892 MS.gene93767:CDS 30.0%
TTGTATTCATAGGAATGTTG+AGG - chr4.2:374736-374755 MS.gene93767:CDS 30.0%
! GATGAAACAACTGGTTTAAT+AGG + chr4.2:374472-374491 None:intergenic 30.0%
! TGTGATTGATATAATGGGAA+AGG - chr4.2:374926-374945 MS.gene93767:CDS 30.0%
!! TTCAAATTTGTTGTTGACTG+TGG + chr4.2:374403-374422 None:intergenic 30.0%
AACACTTGAATACGTTGTAC+AGG - chr4.2:375175-375194 MS.gene93767:CDS 35.0%
GAATTGGGATTTAGAGAGAA+TGG - chr4.2:375113-375132 MS.gene93767:CDS 35.0%
GTACTAAAAACTATGAAGGC+AGG - chr4.2:375062-375081 MS.gene93767:CDS 35.0%
GTGTTTGTGAACACATTTGA+AGG - chr4.2:374966-374985 MS.gene93767:CDS 35.0%
TAAAAACTATGAAGGCAGGA+GGG - chr4.2:375066-375085 MS.gene93767:CDS 35.0%
TATGAATTGAAAATCGGTGG+TGG - chr4.2:374876-374895 MS.gene93767:CDS 35.0%
TATTCATAGGAATGTTGAGG+AGG - chr4.2:374739-374758 MS.gene93767:intron 35.0%
TTCTCTCTAAATCCCAATTC+AGG + chr4.2:375113-375132 None:intergenic 35.0%
! ACAAATAAAAACTCGGCTTG+AGG - chr4.2:374600-374619 MS.gene93767:CDS 35.0%
! ACCAGTTGTTTCATCAACTA+AGG - chr4.2:374477-374496 MS.gene93767:CDS 35.0%
!! AAACCATTGGCTCTAGTTAT+GGG - chr4.2:374996-375015 MS.gene93767:CDS 35.0%
!! ACCTTAGTTGATGAAACAAC+TGG + chr4.2:374481-374500 None:intergenic 35.0%
AAAGAGGTGATTCTGCCTAA+TGG - chr4.2:374764-374783 MS.gene93767:intron 40.0%
AGTTATTGTGCCTCCTGAAT+TGG - chr4.2:375097-375116 MS.gene93767:CDS 40.0%
CTAAAAACTATGAAGGCAGG+AGG - chr4.2:375065-375084 MS.gene93767:CDS 40.0%
GGGGAGTAAGAAAATTGATG+AGG + chr4.2:374299-374318 None:intergenic 40.0%
GTTATTGTGCCTCCTGAATT+GGG - chr4.2:375098-375117 MS.gene93767:CDS 40.0%
TCACACACACCCTTACTATA+TGG + chr4.2:375029-375048 None:intergenic 40.0%
TCTCTAAATCCCAATTCAGG+AGG + chr4.2:375110-375129 None:intergenic 40.0%
TGAAGAAGTGATGTGATGAC+TGG + chr4.2:374332-374351 None:intergenic 40.0%
TGAAGGAGACAAGAAACCAT+TGG - chr4.2:374983-375002 MS.gene93767:CDS 40.0%
TGATGAGGAACCAAAGATAG+TGG + chr4.2:374284-374303 None:intergenic 40.0%
TGATTCTGCCTAATGGCATA+AGG - chr4.2:374771-374790 MS.gene93767:intron 40.0%
! ACGTATTCAAGTGTTGCAAG+TGG + chr4.2:375170-375189 None:intergenic 40.0%
! ATAGTAAGGGTGTGTGTGAA+GGG - chr4.2:375030-375049 MS.gene93767:CDS 40.0%
! GTTGTTGACTGTGGAGATAA+TGG + chr4.2:374394-374413 None:intergenic 40.0%
! TATAGTAAGGGTGTGTGTGA+AGG - chr4.2:375029-375048 MS.gene93767:CDS 40.0%
! TATTCAAGTGTTGCAAGTGG+AGG + chr4.2:375167-375186 None:intergenic 40.0%
!! AACCATTGGCTCTAGTTATG+GGG - chr4.2:374997-375016 MS.gene93767:CDS 40.0%
!! AGAGAGAATGGTGCTGATTT+AGG - chr4.2:375125-375144 MS.gene93767:CDS 40.0%
!! CGACTCATTTTTGTCTCTAC+AGG - chr4.2:374850-374869 MS.gene93767:intron 40.0%
!! GAAACCATTGGCTCTAGTTA+TGG - chr4.2:374995-375014 MS.gene93767:CDS 40.0%
!! GAGAGAATGGTGCTGATTTA+GGG - chr4.2:375126-375145 MS.gene93767:CDS 40.0%
!!! TGGGTTGGTTTTCTTGCATT+TGG - chr4.2:374565-374584 MS.gene93767:CDS 40.0%
AGGAATCTGTTGCTGTGCTT+TGG + chr4.2:374452-374471 None:intergenic 45.0%
GAATGTTGAGGAGGAGAAAG+AGG - chr4.2:374748-374767 MS.gene93767:intron 45.0%
GGGTCAAGGCCATATAGTAA+GGG - chr4.2:375017-375036 MS.gene93767:CDS 45.0%
! AGTGATGTGATGACTGGTTC+TGG + chr4.2:374326-374345 None:intergenic 45.0%
! ATGGGAAAGGTTGAAAGCAC+AGG - chr4.2:374939-374958 MS.gene93767:CDS 45.0%
! CTAAGGTTGATTCCACAGAC+TGG - chr4.2:374494-374513 MS.gene93767:CDS 45.0%
!! AATGGTGCTGATTTAGGGTC+TGG - chr4.2:375131-375150 MS.gene93767:CDS 45.0%
!! GTGATGACTGGTTCTGGTAT+GGG + chr4.2:374320-374339 None:intergenic 45.0%
!! TGATGACTGGTTCTGGTATG+GGG + chr4.2:374319-374338 None:intergenic 45.0%
!! TGTGATGACTGGTTCTGGTA+TGG + chr4.2:374321-374340 None:intergenic 45.0%
!! TGTGGAGATAATGGACCTGA+GGG + chr4.2:374385-374404 None:intergenic 45.0%
AAACCGTCTGGTCAAGGAAG+TGG + chr4.2:374524-374543 None:intergenic 50.0%
ACAACCAAATCACCTCGCCT+TGG + chr4.2:374909-374928 None:intergenic 50.0%
ACTTCCTTGACCAGACGGTT+TGG - chr4.2:374523-374542 MS.gene93767:CDS 50.0%
GACCCCATAACTAGAGCCAA+TGG + chr4.2:375002-375021 None:intergenic 50.0%
GGGGTCAAGGCCATATAGTA+AGG - chr4.2:375016-375035 MS.gene93767:CDS 50.0%
TATGCCAAGGCGAGGTGATT+TGG - chr4.2:374902-374921 MS.gene93767:CDS 50.0%
! TTGCCACTTCCTTGACCAGA+CGG - chr4.2:374518-374537 MS.gene93767:CDS 50.0%
!! CTGTGGAGATAATGGACCTG+AGG + chr4.2:374386-374405 None:intergenic 50.0%
!! GATAATGGACCTGAGGGTTG+CGG + chr4.2:374379-374398 None:intergenic 50.0%
!! GATGACTGGTTCTGGTATGG+GGG + chr4.2:374318-374337 None:intergenic 50.0%
!! TGCTGATTTAGGGTCTGGTG+TGG - chr4.2:375136-375155 MS.gene93767:CDS 50.0%
!! TGGCTCTAGTTATGGGGTCA+AGG - chr4.2:375003-375022 MS.gene93767:CDS 50.0%
AAGGCCAAACCGTCTGGTCA+AGG + chr4.2:374530-374549 None:intergenic 55.0%
CTATGAAGGCAGGAGGGAAG+AGG - chr4.2:375072-375091 MS.gene93767:CDS 55.0%
GGAAGTGGCAATCCAGTCTG+TGG + chr4.2:374509-374528 None:intergenic 55.0%
! TCGGTGGTGGTGATATGCCA+AGG - chr4.2:374889-374908 MS.gene93767:CDS 55.0%
! TTGACCAGACGGTTTGGCCT+TGG - chr4.2:374529-374548 MS.gene93767:CDS 55.0%
!! CCTTGGAGCTGGTCTTGCAT+GGG - chr4.2:374546-374565 MS.gene93767:CDS 55.0%
AGCTCCAAGGCCAAACCGTC+TGG + chr4.2:374536-374555 None:intergenic 60.0%
CCACAACAACCGCAACCCTC+AGG - chr4.2:374367-374386 MS.gene93767:CDS 60.0%
CCCATGCAAGACCAGCTCCA+AGG + chr4.2:374549-374568 None:intergenic 60.0%
CCTGAGGGTTGCGGTTGTTG+TGG + chr4.2:374370-374389 None:intergenic 60.0%
GAGGGTTGCGGTTGTTGTGG+TGG + chr4.2:374367-374386 None:intergenic 60.0%
GGTGGTGATATGCCAAGGCG+AGG - chr4.2:374894-374913 MS.gene93767:CDS 60.0%
GGTTGCGGTTGTTGTGGTGG+AGG + chr4.2:374364-374383 None:intergenic 60.0%
!! AGACGGTTTGGCCTTGGAGC+TGG - chr4.2:374535-374554 MS.gene93767:CDS 60.0%
!! GCCTTGGAGCTGGTCTTGCA+TGG - chr4.2:374545-374564 MS.gene93767:CDS 60.0%
!! GGAGCTGGTCTTGCATGGGT+TGG - chr4.2:374550-374569 MS.gene93767:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 374277 375226 374277 ID=MS.gene93767
chr4.2 mRNA 374277 375226 374277 ID=MS.gene93767.t1;Parent=MS.gene93767
chr4.2 exon 374856 375226 374856 ID=MS.gene93767.t1.exon1;Parent=MS.gene93767.t1
chr4.2 CDS 374856 375226 374856 ID=cds.MS.gene93767.t1;Parent=MS.gene93767.t1
chr4.2 exon 374711 374755 374711 ID=MS.gene93767.t1.exon2;Parent=MS.gene93767.t1
chr4.2 CDS 374711 374755 374711 ID=cds.MS.gene93767.t1;Parent=MS.gene93767.t1
chr4.2 exon 374277 374631 374277 ID=MS.gene93767.t1.exon3;Parent=MS.gene93767.t1
chr4.2 CDS 374277 374631 374277 ID=cds.MS.gene93767.t1;Parent=MS.gene93767.t1
Gene Sequence

>MS.gene93767

ATGGCCACTATCTTTGGTTCCTCATCAATTTTCTTACTCCCCCATACCAGAACCAGTCATCACATCACTTCTTCATCAGCAACTCCTCCACCACAACAACCGCAACCCTCAGGTCCATTATCTCCACAGTCAACAACAAATTTGAATGAAGATCAGTCAGTGCAAGTCTCTGCCAAAGCACAGCAACAGATTCCTATTAAACCAGTTGTTTCATCAACTAAGGTTGATTCCACAGACTGGATTGCCACTTCCTTGACCAGACGGTTTGGCCTTGGAGCTGGTCTTGCATGGGTTGGTTTTCTTGCATTTGGTGTTGTCTCAGAACAAATAAAAACTCGGCTTGAGGTTTCACAACAAGAATCTAATACAAGGTTTCTCACTGTTACATCTTTTTCTATGTCTTACTACATTTATCAAAATCATATATGAATGGTTACATGTTAGTTAATAATGATATTTTTGTATTCATAGGAATGTTGAGGAGGAGAAAGAGGTGATTCTGCCTAATGGCATAAGGTTAATTATTTCACACACTATGATTAGAAGATTTGAAAATTACATACAAATATTGTGCGACTCATTTTTGTCTCTACAGGTACTATGAATTGAAAATCGGTGGTGGTGATATGCCAAGGCGAGGTGATTTGGTTGTGATTGATATAATGGGAAAGGTTGAAAGCACAGGAGAAGTGTTTGTGAACACATTTGAAGGAGACAAGAAACCATTGGCTCTAGTTATGGGGTCAAGGCCATATAGTAAGGGTGTGTGTGAAGGGATTGAATATGTACTAAAAACTATGAAGGCAGGAGGGAAGAGGAAAGTTATTGTGCCTCCTGAATTGGGATTTAGAGAGAATGGTGCTGATTTAGGGTCTGGTGTGGAAATTCCTCCACTTGCAACACTTGAATACGTTGTACAGGTTGATAAAGTGTCCATTGCACCTGCTTGA

Protein sequence

>MS.gene93767.t1

MATIFGSSSIFLLPHTRTSHHITSSSATPPPQQPQPSGPLSPQSTTNLNEDQSVQVSAKAQQQIPIKPVVSSTKVDSTDWIATSLTRRFGLGAGLAWVGFLAFGVVSEQIKTRLEVSQQESNTRNVEEEKEVILPNGIRYYELKIGGGDMPRRGDLVVIDIMGKVESTGEVFVNTFEGDKKPLALVMGSRPYSKGVCEGIEYVLKTMKAGGKRKVIVPPELGFRENGADLGSGVEIPPLATLEYVVQVDKVSIAPA