Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006559.01.T01 | XP_003593369.2 | 91.981 | 212 | 17 | 0 | 157 | 368 | 23 | 234 | 2.34E-139 | 411 |
| MsG0280006559.01.T01 | XP_003593369.2 | 76.699 | 103 | 23 | 1 | 22 | 123 | 147 | 249 | 5.07E-44 | 166 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048352.01 | MsG0280006559.01 | 0.800839 | 1.193922e-48 | 5.616191e-46 |
| MsG0080048889.01 | MsG0280006559.01 | 0.818689 | 1.719772e-52 | 1.296197e-49 |
| MsG0080048980.01 | MsG0280006559.01 | 0.813222 | 2.854511e-51 | 1.853075e-48 |
| MsG0080049023.01 | MsG0280006559.01 | -0.804041 | 2.613117e-49 | 1.333687e-46 |
| MsG0080049066.01 | MsG0280006559.01 | -0.801419 | 9.082985e-49 | 4.335656e-46 |
| MsG0180001008.01 | MsG0280006559.01 | 0.801886 | 7.286168e-49 | 3.519458e-46 |
| MsG0180001513.01 | MsG0280006559.01 | 0.831505 | 1.607116e-55 | 1.747503e-52 |
| MsG0180001515.01 | MsG0280006559.01 | 0.805446 | 1.329489e-49 | 7.035162e-47 |
| MsG0180001691.01 | MsG0280006559.01 | 0.803054 | 4.185546e-49 | 2.082644e-46 |
| MsG0180003235.01 | MsG0280006559.01 | 0.821466 | 3.977316e-53 | 3.238241e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006559.01.T01 | MTR_2g010710 | 91.981 | 212 | 17 | 0 | 157 | 368 | 23 | 234 | 2.83e-143 | 411 |
| MsG0280006559.01.T01 | MTR_2g010710 | 76.699 | 103 | 23 | 1 | 22 | 123 | 147 | 249 | 6.14e-48 | 166 |
| MsG0280006559.01.T01 | MTR_2g010730 | 75.115 | 217 | 49 | 2 | 157 | 368 | 23 | 239 | 4.79e-114 | 337 |
| MsG0280006559.01.T01 | MTR_2g010730 | 66.667 | 129 | 41 | 2 | 22 | 149 | 152 | 279 | 2.74e-51 | 176 |
| MsG0280006559.01.T01 | MTR_2g010720 | 73.239 | 213 | 54 | 3 | 157 | 368 | 87 | 297 | 5.93e-103 | 311 |
| MsG0280006559.01.T01 | MTR_2g010720 | 97.143 | 105 | 3 | 0 | 19 | 123 | 208 | 312 | 4.79e-67 | 219 |
| MsG0280006559.01.T01 | MTR_3g011910 | 33.898 | 177 | 103 | 3 | 200 | 367 | 79 | 250 | 1.56e-31 | 127 |
| MsG0280006559.01.T01 | MTR_3g011910 | 31.858 | 113 | 72 | 2 | 10 | 117 | 148 | 260 | 4.92e-11 | 65.1 |
| MsG0280006559.01.T01 | MTR_3g011910 | 33.898 | 177 | 103 | 3 | 200 | 367 | 53 | 224 | 2.25e-31 | 128 |
| MsG0280006559.01.T01 | MTR_3g011910 | 31.858 | 113 | 72 | 2 | 10 | 117 | 122 | 234 | 3.80e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006559.01.T01 | AT1G61750 | 30.198 | 202 | 115 | 5 | 180 | 367 | 49 | 238 | 2.63e-20 | 92.4 |
| MsG0280006559.01.T01 | AT4G23170 | 32.642 | 193 | 113 | 7 | 184 | 365 | 49 | 235 | 6.94e-20 | 89.7 |
| MsG0280006559.01.T01 | AT4G05200 | 31.579 | 190 | 121 | 5 | 184 | 365 | 50 | 238 | 8.06e-20 | 93.2 |
| MsG0280006559.01.T01 | AT4G05200 | 31.579 | 190 | 121 | 5 | 184 | 365 | 68 | 256 | 8.11e-20 | 93.2 |
| MsG0280006559.01.T01 | AT3G22060 | 29.534 | 193 | 126 | 6 | 181 | 365 | 49 | 239 | 2.56e-18 | 85.1 |
| MsG0280006559.01.T01 | AT4G23180 | 31.373 | 204 | 127 | 5 | 172 | 362 | 48 | 251 | 2.21e-17 | 85.5 |
| MsG0280006559.01.T01 | AT4G23180 | 31.527 | 203 | 126 | 5 | 173 | 362 | 49 | 251 | 3.05e-17 | 84.7 |
| MsG0280006559.01.T01 | AT5G48540 | 27.602 | 221 | 128 | 6 | 168 | 367 | 24 | 233 | 3.59e-17 | 81.6 |
| MsG0280006559.01.T01 | AT4G23180 | 31.373 | 204 | 127 | 5 | 172 | 362 | 48 | 251 | 3.67e-17 | 84.7 |
| MsG0280006559.01.T01 | AT4G23160 | 31.088 | 193 | 116 | 6 | 184 | 365 | 66 | 252 | 1.45e-16 | 83.2 |
Find 108 sgRNAs with CRISPR-Local
Find 295 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGAAGATGGGAAATTTA+TGG | 0.230790 | 2:-3436590 | MsG0280006559.01.T01:CDS |
| AAAGGACTGCAAAAGGTGTC+TGG | 0.422008 | 2:-3439596 | MsG0280006559.01.T01:CDS |
| AACCCTTGTGAAACCACAGA+AGG | 0.649733 | 2:+3439745 | None:intergenic |
| AACCCTTGTGAAACTAAAGA+AGG | 0.525606 | 2:+3436800 | None:intergenic |
| AACTCTCGTCCGAATAGCGT+AGG | 0.629050 | 2:+3436882 | None:intergenic |
| AAGAAATGTGAATCAGAAGT+TGG | 0.446729 | 2:+3435398 | None:intergenic |
| AAGGTGCCTTGATGATCAAC+TGG | 0.464509 | 2:-3436639 | MsG0280006559.01.T01:CDS |
| AAGTATCCAGTTGATCATCA+AGG | 0.576954 | 2:+3436633 | None:intergenic |
| AAGTGAGAAGAGATATGGAA+TGG | 0.598662 | 2:-3436699 | MsG0280006559.01.T01:CDS |
| AAGTGAGAAGAGGTATGGAA+TGG | 0.598662 | 2:-3439644 | MsG0280006559.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAGCTAGTTCGAATGATA+AGG | + | Chr2:3437523-3437542 | None:intergenic | 30.0% | |
| AAATGACTTCATAGTCTCTT+TGG | + | Chr2:3437804-3437823 | None:intergenic | 30.0% | |
| AAATGTGTCCATAAACCAAA+AGG | - | Chr2:3436616-3436635 | MsG0280006559.01.T01:CDS | 30.0% | |
| AAGAAATGTGAATCAGAAGT+TGG | + | Chr2:3439666-3439685 | None:intergenic | 30.0% | |
| AATCAAAGTGAGAAGAGATA+TGG | - | Chr2:3438357-3438376 | MsG0280006559.01.T01:intron | 30.0% | |
| ACTATATTTGCTTCCTTAAC+CGG | - | Chr2:3436798-3436817 | MsG0280006559.01.T01:CDS | 30.0% | |
| AGACATATTCCATACCTTAT+TGG | - | Chr2:3437951-3437970 | MsG0280006559.01.T01:intron | 30.0% | |
| ATATGACAAAAGAAGTTGTC+GGG | + | Chr2:3436950-3436969 | None:intergenic | 30.0% | |
| ATTCACATTTCTTACCATCT+CGG | - | Chr2:3439673-3439692 | MsG0280006559.01.T01:CDS | 30.0% | |
| ATTGAAAGTTATCCCACTTA+TGG | + | Chr2:3439098-3439117 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 3435241 | 3439842 | 3435241 | ID=MsG0280006559.01;Name=MsG0280006559.01 |
| Chr2 | mRNA | 3435241 | 3439842 | 3435241 | ID=MsG0280006559.01.T01;Parent=MsG0280006559.01;Name=MsG0280006559.01.T01;_AED=0.53;_eAED=0.54;_QI=0|0|0|0.4|1|1|5|0|479 |
| Chr2 | exon | 3439473 | 3439842 | 3439473 | ID=MsG0280006559.01.T01:exon:1689;Parent=MsG0280006559.01.T01 |
| Chr2 | exon | 3438247 | 3438317 | 3438247 | ID=MsG0280006559.01.T01:exon:1688;Parent=MsG0280006559.01.T01 |
| Chr2 | exon | 3436572 | 3437233 | 3436572 | ID=MsG0280006559.01.T01:exon:1687;Parent=MsG0280006559.01.T01 |
| Chr2 | exon | 3436161 | 3436273 | 3436161 | ID=MsG0280006559.01.T01:exon:1686;Parent=MsG0280006559.01.T01 |
| Chr2 | exon | 3435241 | 3435464 | 3435241 | ID=MsG0280006559.01.T01:exon:1685;Parent=MsG0280006559.01.T01 |
| Chr2 | CDS | 3439473 | 3439842 | 3439473 | ID=MsG0280006559.01.T01:cds;Parent=MsG0280006559.01.T01 |
| Chr2 | CDS | 3438247 | 3438317 | 3438247 | ID=MsG0280006559.01.T01:cds;Parent=MsG0280006559.01.T01 |
| Chr2 | CDS | 3436572 | 3437233 | 3436572 | ID=MsG0280006559.01.T01:cds;Parent=MsG0280006559.01.T01 |
| Chr2 | CDS | 3436161 | 3436273 | 3436161 | ID=MsG0280006559.01.T01:cds;Parent=MsG0280006559.01.T01 |
| Chr2 | CDS | 3435241 | 3435464 | 3435241 | ID=MsG0280006559.01.T01:cds;Parent=MsG0280006559.01.T01 |
| Gene Sequence |
>MsG0280006559.01.T01 ATGAGCCTCATTTTACTTCCGGCACCGAGCCTCCAAATTCGTTGGAACGGCCCTGACTTTGATGATATTGATGATCCTTCTGTGGTTTCACAAGGGTTACCTTTCATGAGTGGAGTAGCAGCTACAGCTTCAGAGAAATCATTAATGTTCCACACAGAAGTACTGAACTTCAATCAAAGTGAGAAGAGGTATGGAATGGCTCAGTGCACAAGAGATATCAGTAGAAAGGACTGCAAAAGGTGTCTGGATTCTCAGCTGATTAATCACAGAACAATTAGAAATAATACAAGATGGGAAATTTATGGATCCAGTTGTTTCATGTGGTATGATGACTATCAGTTTTATGCTAATGTATCCTTTCTTCCAAGTGAAATTGATTACACAATTTCCGATGTAAAAGATACTATATTTGCTTCCTTAACCGGTGCCCCGGGGGCACTGCTTCTTTGTTGTAGTCTTTCCAAATGGCTTTTTGGCGTTGCTGATGACACCAATAGAGAAATAGGTTCGATATGTAATAAAGCTGATAATACGGTCAATGAAGACATATTCCATACCTTATTGGATTCTCTTGCTTCAAATGTGGTTGATCACCAAGGATTTTACCAAACCATTGCAGGAGAAAACTCCAGCATGGTTTATGGTTCGATATTATGCAGAGGAGACATATCTGCTAACAATTGTTCTGTTTGTGCTCTTAATTCTACAAGAGTAGCTTCAAATGATTGTCCTAAGAATAGAGATGTTATAGTTTGGTTTAGTTGGTGTTTCCTACGCTATTCGGACGAGAGTTTCTTCGGAGACATGCATGGAATAACAAATGCATTCTCAAATGTCACTGATATTGATGACCCTTCTTTAGTTTCACAAGGGTTACCTTTCATGAGTGGAGTAGCAGCTGCAGCTTCAGAGAACTCATTCATGTTCCACACAGAAGTACTGAACTTCAATCAAAGTGAGAAGAGATATGGAATGGCTCAGTGCACAAGAGATATCAATAGACAGGACTGCAGAAGGTGCCTTGATGATCAACTGGATACTTTCAGAGCTACAATTGGAAATAAAAGAAGATGGGAAATTTATGGACCCAATTGTTTCATGTGGCCATGTCCTATCCGATGTGGGACTCTTAAGACACCCCCTCATGACCAGCACTATTGGGCTTGGTTCGTGGACATATATGGTGGGTGGCCCGATAACGGAAACCTGATAGCAGGGTTCCGAGAGTCAAATTCGATTCCTCTTGTTTTTGCGTTTAATCCAACTTCTGATTCACATTTCTTACCATCTCGGGACAATGTATCTGTTCCAAGCTCCAACGAGTTTTTCTCAGATGAAGATACCATCAGTGATGCTTCAGACGTCACCGATGCAAATGACATTCCCATTGAAAAAAGTTTGGTTCAAGATGCTTTGGACTATGGTTTGTTTTTGGTGTGA |
| Protein sequence |
>MsG0280006559.01.T01 MSLILLPAPSLQIRWNGPDFDDIDDPSVVSQGLPFMSGVAATASEKSLMFHTEVLNFNQSEKRYGMAQCTRDISRKDCKRCLDSQLINHRTIRNNTRWEIYGSSCFMWYDDYQFYANVSFLPSEIDYTISDVKDTIFASLTGAPGALLLCCSLSKWLFGVADDTNREIGSICNKADNTVNEDIFHTLLDSLASNVVDHQGFYQTIAGENSSMVYGSILCRGDISANNCSVCALNSTRVASNDCPKNRDVIVWFSWCFLRYSDESFFGDMHGITNAFSNVTDIDDPSLVSQGLPFMSGVAAAASENSFMFHTEVLNFNQSEKRYGMAQCTRDINRQDCRRCLDDQLDTFRATIGNKRRWEIYGPNCFMWPCPIRCGTLKTPPHDQHYWAWFVDIYGGWPDNGNLIAGFRESNSIPLVFAFNPTSDSHFLPSRDNVSVPSSNEFFSDEDTISDASDVTDANDIPIEKSLVQDALDYGLFLV* |
