AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006766.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006766.01.T01 MTR_2g014440 86.471 170 19 1 1 170 1 166 1.38e-97 286
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006766.01.T01 AT1G28410 44.375 160 82 2 11 163 1 160 1.85e-27 106
MsG0280006766.01.T01 AT1G28410 44.375 160 82 2 11 163 1 160 1.85e-27 106

Find 31 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACATAGGTACAACTTCTT+TGG 0.235233 2:-5964802 MsG0280006766.01.T01:intron
AAAGAGAGCTAATAAGAATA+AGG 0.323248 2:+5965608 None:intergenic
TACATAGGTACAACTTCTTT+GGG 0.348459 2:-5964801 MsG0280006766.01.T01:intron
AGAATAAGCTTTAGATACTT+TGG 0.374804 2:+5963758 None:intergenic
AATGGTAATGGTAATGGAAT+TGG 0.378613 2:+5965632 None:intergenic
TCTATTGATTCAGCTGAAAC+TGG 0.388503 2:+5965567 None:intergenic
AAGAAGATGCACATTCTTGA+AGG 0.416371 2:-5963618 MsG0280006766.01.T01:CDS
GGTTACTTCAAGAGTTGAAA+AGG 0.444874 2:-5964702 MsG0280006766.01.T01:intron
TAAGGTAATGGTAATGGTAA+TGG 0.453497 2:+5965626 None:intergenic
TAAGAATAAGGTAATGGTAA+TGG 0.474570 2:+5965620 None:intergenic
GGACGATAATGAAAAGAGTC+TGG 0.477854 2:-5964729 MsG0280006766.01.T01:CDS
TGCAAAGATGAGGGCTCTAA+AGG 0.489938 2:-5963660 MsG0280006766.01.T01:CDS
ATCAAAGTTGTTCTTTCTCA+AGG 0.493797 2:+5964775 None:intergenic
AGCTAATAAGAATAAGGTAA+TGG 0.496744 2:+5965614 None:intergenic
CATTAAAGCTCTGGAGGAAG+AGG 0.496876 2:-5964920 MsG0280006766.01.T01:intron
CTCTACTCTCTCTTCAATCA+AGG 0.503720 2:-5965311 MsG0280006766.01.T01:intron
TTCTTATTGTGCAAAGATGA+GGG 0.516355 2:-5963669 MsG0280006766.01.T01:CDS
TTTCTTATTGTGCAAAGATG+AGG 0.519016 2:-5963670 MsG0280006766.01.T01:CDS
TCAAGTTCAGCATCTTGAGC+AGG 0.524922 2:-5963793 MsG0280006766.01.T01:CDS
CAATCAAAAGCTAACTGAAA+GGG 0.528049 2:-5965398 MsG0280006766.01.T01:CDS
TGAGGGCTCTAAAGGCTCAA+TGG 0.537828 2:-5963652 MsG0280006766.01.T01:CDS
TAATGAAAAGAGTCTGGAAG+AGG 0.556127 2:-5964723 MsG0280006766.01.T01:CDS
CCAATCAAAAGCTAACTGAA+AGG 0.558795 2:-5965399 MsG0280006766.01.T01:CDS
TGAGACACACATTAAAGCTC+TGG 0.562782 2:-5964929 MsG0280006766.01.T01:CDS
AACCTCAAAATCGCTCACTT+AGG 0.579766 2:-5965516 MsG0280006766.01.T01:intron
AACAGCAGAGTGCAAAGCAG+AGG 0.585005 2:-5965692 MsG0280006766.01.T01:five_prime_UTR
CCATATCTTAAAATCTAAAG+CGG 0.598538 2:-5964750 MsG0280006766.01.T01:CDS
GACACACATTAAAGCTCTGG+AGG 0.639572 2:-5964926 MsG0280006766.01.T01:CDS
GAGAAGCATCACATTCGCAA+TGG 0.646241 2:-5965661 MsG0280006766.01.T01:exon
GTAATGGTAATGGAATTGGA+AGG 0.663918 2:+5965636 None:intergenic
GTGGCATTGTGACTGAACAG+TGG 0.727418 2:-5963818 MsG0280006766.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATATTAACTAATGCA+AGG + Chr2:5965418-5965437 None:intergenic 15.0%
!! AAATATATATTTCATTGCTT+CGG + Chr2:5964769-5964788 None:intergenic 15.0%
!! TGAATAAATTTATGTAAAAG+TGG + Chr2:5964129-5964148 None:intergenic 15.0%
!! TTGGTATATAAATATAATGA+CGG - Chr2:5964687-5964706 MsG0280006766.01.T01:intron 15.0%
!!! ATTCTAAAATATTCTTGTTT+AGG - Chr2:5964956-5964975 MsG0280006766.01.T01:intron 15.0%
!!! ATTTTTTTCTACTCATTAAT+TGG - Chr2:5965366-5965385 MsG0280006766.01.T01:CDS 15.0%
!! AAATTATTTGAATGCAGAAT+CGG - Chr2:5963841-5963860 MsG0280006766.01.T01:intron 20.0%
!! AATATATATTTCATTGCTTC+GGG + Chr2:5964768-5964787 None:intergenic 20.0%
!! AATTATACACTAGATCAAAA+CGG + Chr2:5965277-5965296 None:intergenic 20.0%
!! TAAAGTAATCAAATTCATCT+TGG + Chr2:5965333-5965352 None:intergenic 20.0%
!! TGGTATATAAATATAATGAC+GGG - Chr2:5964688-5964707 MsG0280006766.01.T01:intron 20.0%
!! TTATTGAGAAGAGTAAAATT+AGG + Chr2:5964012-5964031 None:intergenic 20.0%
!!! TTCAAAATCAAATTTTATCC+AGG - Chr2:5964202-5964221 MsG0280006766.01.T01:intron 20.0%
!!! TTTAGAATGATCATGTATTT+CGG + Chr2:5964944-5964963 None:intergenic 20.0%
! AAAGAGAGCTAATAAGAATA+AGG + Chr2:5963670-5963689 None:intergenic 25.0%
! AAATGCAACATAAAATTCGA+AGG + Chr2:5963812-5963831 None:intergenic 25.0%
! ACTAATACTATGTATGGTAT+TGG - Chr2:5964668-5964687 MsG0280006766.01.T01:intron 25.0%
! AGCTAATAAGAATAAGGTAA+TGG + Chr2:5963664-5963683 None:intergenic 25.0%
! ATAGTGAATGATTTATGTTC+TGG - Chr2:5964418-5964437 MsG0280006766.01.T01:intron 25.0%
! ATGGTTACTAATACTATGTA+TGG - Chr2:5964662-5964681 MsG0280006766.01.T01:intron 25.0%
! CCATATCTTAAAATCTAAAG+CGG - Chr2:5964525-5964544 MsG0280006766.01.T01:intron 25.0%
! TTAATTCCTGTTAAACTTCT+TGG - Chr2:5964382-5964401 MsG0280006766.01.T01:intron 25.0%
!! AAAATGTGTTATTATGCTTG+CGG - Chr2:5964248-5964267 MsG0280006766.01.T01:intron 25.0%
!! AATGTATTGATCTTTCTTGT+AGG - Chr2:5964307-5964326 MsG0280006766.01.T01:intron 25.0%
!! ATATTATGTTCTGCACATTT+TGG - Chr2:5964634-5964653 MsG0280006766.01.T01:intron 25.0%
!! GCTTCAATTAGAATCAATTT+TGG + Chr2:5964086-5964105 None:intergenic 25.0%
!! TAAGAATAAGGTAATGGTAA+TGG + Chr2:5963658-5963677 None:intergenic 25.0%
!! TCAATTAGAATCAATTTTGG+TGG + Chr2:5964083-5964102 None:intergenic 25.0%
!! TTTGTAAACCAAATTAGTCT+TGG + Chr2:5964984-5965003 None:intergenic 25.0%
!!! AGAATAAGCTTTAGATACTT+TGG + Chr2:5965520-5965539 None:intergenic 25.0%
!!! ATATTGTTTCACTTTTTCAG+CGG - Chr2:5965009-5965028 MsG0280006766.01.T01:intron 25.0%
!!! TTATTTCTGTAACATGCATA+AGG + Chr2:5964724-5964743 None:intergenic 25.0%
AAACAACCAAGAAGTTTAAC+AGG + Chr2:5964391-5964410 None:intergenic 30.0%
AATAACTGTACAATGAGGAT+CGG - Chr2:5964857-5964876 MsG0280006766.01.T01:intron 30.0%
ATACATAGGTACAACTTCTT+TGG - Chr2:5964473-5964492 MsG0280006766.01.T01:intron 30.0%
ATATCAGCTGAAATCTTTGT+GGG + Chr2:5965304-5965323 None:intergenic 30.0%
ATCAAAGTTGTTCTTTCTCA+AGG + Chr2:5964503-5964522 None:intergenic 30.0%
CAATCAAAAGCTAACTGAAA+GGG - Chr2:5963877-5963896 MsG0280006766.01.T01:intron 30.0%
CTACTCATTAATTGGAGATA+TGG - Chr2:5965374-5965393 MsG0280006766.01.T01:CDS 30.0%
TACATAGGTACAACTTCTTT+GGG - Chr2:5964474-5964493 MsG0280006766.01.T01:intron 30.0%
TATATCAGCTGAAATCTTTG+TGG + Chr2:5965305-5965324 None:intergenic 30.0%
TTAGCTAACCAACAACTAAT+CGG - Chr2:5965174-5965193 MsG0280006766.01.T01:intron 30.0%
TTCTTATTGTGCAAAGATGA+GGG - Chr2:5965606-5965625 MsG0280006766.01.T01:CDS 30.0%
TTTCTTATTGTGCAAAGATG+AGG - Chr2:5965605-5965624 MsG0280006766.01.T01:CDS 30.0%
! AGCTTAAAGAGTGTAAGATA+AGG - Chr2:5965084-5965103 MsG0280006766.01.T01:intron 30.0%
! ATCTAGAAGAGTGTGAAAAA+CGG - Chr2:5963906-5963925 MsG0280006766.01.T01:intron 30.0%
! GTATTTCGGAGAAATTGTTT+TGG + Chr2:5964930-5964949 None:intergenic 30.0%
!! AATGGTAATGGTAATGGAAT+TGG + Chr2:5963646-5963665 None:intergenic 30.0%
!! TAAGGTAATGGTAATGGTAA+TGG + Chr2:5963652-5963671 None:intergenic 30.0%
!! TTTCAACTGATTTGAGGAAT+AGG - Chr2:5964443-5964462 MsG0280006766.01.T01:intron 30.0%
!!! CCGCTTTAGATTTTAAGATA+TGG + Chr2:5964528-5964547 None:intergenic 30.0%
!!! TATTGTTTCACTTTTTCAGC+GGG - Chr2:5965010-5965029 MsG0280006766.01.T01:intron 30.0%
AAGAAGATGCACATTCTTGA+AGG - Chr2:5965657-5965676 MsG0280006766.01.T01:exon 35.0%
CCAATCAAAAGCTAACTGAA+AGG - Chr2:5963876-5963895 MsG0280006766.01.T01:intron 35.0%
GAATAGGTGTGGAAATACAT+AGG - Chr2:5964459-5964478 MsG0280006766.01.T01:intron 35.0%
GACACAATAACTGTACAATG+AGG - Chr2:5964852-5964871 MsG0280006766.01.T01:intron 35.0%
GAGAAGAGTAAAATTAGGTC+AGG + Chr2:5964007-5964026 None:intergenic 35.0%
GGTTACTTCAAGAGTTGAAA+AGG - Chr2:5964573-5964592 MsG0280006766.01.T01:intron 35.0%
TAATGAAAAGAGTCTGGAAG+AGG - Chr2:5964552-5964571 MsG0280006766.01.T01:intron 35.0%
TTTAGGCTCCAAGACTAATT+TGG - Chr2:5964973-5964992 MsG0280006766.01.T01:intron 35.0%
! AGTTGTTGGTTAGCTAAGAA+AGG + Chr2:5965171-5965190 None:intergenic 35.0%
! CTTGATTCTAGCTAAAAGTG+AGG + Chr2:5964179-5964198 None:intergenic 35.0%
! TTCTTTTCCATTCAGATGAG+TGG - Chr2:5965438-5965457 MsG0280006766.01.T01:intron 35.0%
!! GTAATGGTAATGGAATTGGA+AGG + Chr2:5963642-5963661 None:intergenic 35.0%
!! TAGCACTTTGTTGTTTGAGT+AGG - Chr2:5965053-5965072 MsG0280006766.01.T01:intron 35.0%
!! TCACTGTGTTTGATTTTACC+TGG + Chr2:5964223-5964242 None:intergenic 35.0%
!! TCTATTGATTCAGCTGAAAC+TGG + Chr2:5963711-5963730 None:intergenic 35.0%
!! TCTGCACATTTTGGTCATAA+TGG - Chr2:5964643-5964662 MsG0280006766.01.T01:intron 35.0%
!!! ATTGTTTCACTTTTTCAGCG+GGG - Chr2:5965011-5965030 MsG0280006766.01.T01:intron 35.0%
!!! CCTTTCAGTTAGCTTTTGAT+TGG + Chr2:5963879-5963898 None:intergenic 35.0%
!!! CTGGATTTTCAACTGATTTG+AGG - Chr2:5964437-5964456 MsG0280006766.01.T01:intron 35.0%
AACCTCAAAATCGCTCACTT+AGG - Chr2:5963759-5963778 MsG0280006766.01.T01:CDS 40.0%
CTCTACTCTCTCTTCAATCA+AGG - Chr2:5963964-5963983 MsG0280006766.01.T01:intron 40.0%
GGACGATAATGAAAAGAGTC+TGG - Chr2:5964546-5964565 MsG0280006766.01.T01:intron 40.0%
TGAGACACACATTAAAGCTC+TGG - Chr2:5964346-5964365 MsG0280006766.01.T01:intron 40.0%
! AGAAGAGTGTGAAAAACGGA+TGG - Chr2:5963910-5963929 MsG0280006766.01.T01:intron 40.0%
! ATGTGTTATTATGCTTGCGG+AGG - Chr2:5964251-5964270 MsG0280006766.01.T01:intron 40.0%
! TACCTAAGTGAGCGATTTTG+AGG + Chr2:5963764-5963783 None:intergenic 40.0%
!! ACTGATTTGAGGAATAGGTG+TGG - Chr2:5964448-5964467 MsG0280006766.01.T01:intron 40.0%
!!! GAGCGATTTTGAGGTTGATT+TGG + Chr2:5963755-5963774 None:intergenic 40.0%
!!! TTGTTTCACTTTTTCAGCGG+GGG - Chr2:5965012-5965031 MsG0280006766.01.T01:intron 40.0%
AACCAACAACTAATCGGCAC+TGG - Chr2:5965180-5965199 MsG0280006766.01.T01:intron 45.0%
CACAATGCCACTCATCTGAA+TGG + Chr2:5965448-5965467 None:intergenic 45.0%
TCAAGTTCAGCATCTTGAGC+AGG - Chr2:5965482-5965501 MsG0280006766.01.T01:intron 45.0%
! GACACACATTAAAGCTCTGG+AGG - Chr2:5964349-5964368 MsG0280006766.01.T01:intron 45.0%
! TGCAAAGATGAGGGCTCTAA+AGG - Chr2:5965615-5965634 MsG0280006766.01.T01:CDS 45.0%
!! AACAACTAATCGGCACTGGT+GGG - Chr2:5965184-5965203 MsG0280006766.01.T01:intron 45.0%
!! CATTAAAGCTCTGGAGGAAG+AGG - Chr2:5964355-5964374 MsG0280006766.01.T01:intron 45.0%
!! GAGAAGCATCACATTCGCAA+TGG - Chr2:5963614-5963633 MsG0280006766.01.T01:CDS 45.0%
AACAGCAGAGTGCAAAGCAG+AGG - Chr2:5963583-5963602 MsG0280006766.01.T01:CDS 50.0%
GTGGCATTGTGACTGAACAG+TGG - Chr2:5965457-5965476 MsG0280006766.01.T01:intron 50.0%
! CAACAACTAATCGGCACTGG+TGG - Chr2:5965183-5965202 MsG0280006766.01.T01:intron 50.0%
!! CACCAGTGCCGATTAGTTGT+TGG + Chr2:5965185-5965204 None:intergenic 50.0%
!! TGAGGGCTCTAAAGGCTCAA+TGG - Chr2:5965623-5965642 MsG0280006766.01.T01:CDS 50.0%
!! CTAATCGGCACTGGTGGGCA+TGG - Chr2:5965189-5965208 MsG0280006766.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 5963569 5965728 5963569 ID=MsG0280006766.01;Name=MsG0280006766.01
Chr2 mRNA 5963569 5965728 5963569 ID=MsG0280006766.01.T01;Parent=MsG0280006766.01;Name=MsG0280006766.01.T01;_AED=0.48;_eAED=0.57;_QI=64|1|0.8|1|0.75|0.8|5|0|237
Chr2 exon 5963569 5963844 5963569 ID=MsG0280006766.01.T01:exon:2415;Parent=MsG0280006766.01.T01
Chr2 exon 5964703 5964816 5964703 ID=MsG0280006766.01.T01:exon:2414;Parent=MsG0280006766.01.T01
Chr2 exon 5964921 5964968 5964921 ID=MsG0280006766.01.T01:exon:2413;Parent=MsG0280006766.01.T01
Chr2 exon 5965312 5965439 5965312 ID=MsG0280006766.01.T01:exon:2412;Parent=MsG0280006766.01.T01
Chr2 exon 5965517 5965728 5965517 ID=MsG0280006766.01.T01:exon:2411;Parent=MsG0280006766.01.T01
Chr2 five_prime_UTR 5965665 5965728 5965665 ID=MsG0280006766.01.T01:five_prime_utr;Parent=MsG0280006766.01.T01
Chr2 CDS 5965517 5965664 5965517 ID=MsG0280006766.01.T01:cds;Parent=MsG0280006766.01.T01
Chr2 CDS 5965312 5965439 5965312 ID=MsG0280006766.01.T01:cds;Parent=MsG0280006766.01.T01
Chr2 CDS 5964921 5964968 5964921 ID=MsG0280006766.01.T01:cds;Parent=MsG0280006766.01.T01
Chr2 CDS 5964703 5964816 5964703 ID=MsG0280006766.01.T01:cds;Parent=MsG0280006766.01.T01
Chr2 CDS 5963569 5963844 5963569 ID=MsG0280006766.01.T01:cds;Parent=MsG0280006766.01.T01
Gene Sequence

>MsG0280006766.01.T01

ATGGTTCCTTCCAATTCCATTACCATTACCATTACCTTATTCTTATTAGCTCTCTTTCTTGTTCAAACTTCGCTTCCAGTTTCAGCTGAATCAATAGATCACTTCAACTCTCAGATCAACCAAATCAACCTCAAAATCGCTCACTTAGAATCGGTTCTTGAACAAACCAATCAAAAGCTAACTGAAAGGGATTTGTATCTAGAAGAGTGTGAAAAACGGATGGATCACATGTCTGAGAAAATTCTTCATCTGCACTCTACTCTCTCTTCAATCAAGGATGATTCATTGCGTGCTGAGACACACATTAAAGCTCTGGAGGAAGAGGTACAACTTCTTTGGGCTGCCTTGAGAAAGAACAACTTTGATCTCCATATCTTAAAATCTAAAGCGGACGATAATGAAAAGAGTCTGGAAGAGGTTACTTCAAGAGTTGAAAAGATGAGTGGCATTGTGACTGAACAGTGGATTCAAGTTCAGCATCTTGAGCAGGCTCTTCACATTGCCAAAGTATCTAAAGCTTATTCTCTATTGTATAATTTTTTTGTAAAAGTTAACAAGTTTAAATTTGTGTTTTTGAGCATGTCATTTATTTTCTTATTGTGCAAAGATGAGGGCTCTAAAGGCTCAATGGCAAGTGAGCTTAAGAAGATGCACATTCTTGAAGGTAACTTAATTGTTCAGTGTAAGAACATTTATATATGTCATGAATCATGA

Protein sequence

>MsG0280006766.01.T01

MVPSNSITITITLFLLALFLVQTSLPVSAESIDHFNSQINQINLKIAHLESVLEQTNQKLTERDLYLEECEKRMDHMSEKILHLHSTLSSIKDDSLRAETHIKALEEEVQLLWAALRKNNFDLHILKSKADDNEKSLEEVTSRVEKMSGIVTEQWIQVQHLEQALHIAKVSKAYSLLYNFFVKVNKFKFVFLSMSFIFLLCKDEGSKGSMASELKKMHILEGNLIVQCKNIYICHES*