AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017440.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017440.01.T01 MTR_3g107610 96.992 133 3 1 1 133 1 132 2.03e-80 236
MsG0380017440.01.T01 MTR_3g107610 89.726 146 14 1 1 146 1 145 4.00e-80 235
MsG0380017440.01.T01 MTR_4g011190 59.398 133 38 4 1 133 1 117 4.41e-37 125
MsG0380017440.01.T01 MTR_4g113840 39.416 137 40 6 5 133 6 107 2.43e-18 77.4
MsG0380017440.01.T01 MTR_2g054750 36.893 103 46 2 5 106 6 90 9.27e-18 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017440.01.T01 AT3G15680 74.468 94 21 2 1 94 1 91 3.93e-42 139
MsG0380017440.01.T01 AT3G15680 74.468 94 21 2 1 94 1 91 3.93e-42 139
MsG0380017440.01.T01 AT5G25490 65.049 103 32 2 1 103 1 99 8.36e-38 128
MsG0380017440.01.T01 AT5G25490 65.049 103 32 2 1 103 1 99 8.36e-38 128
MsG0380017440.01.T01 AT2G26695 38.760 129 51 5 5 133 6 106 6.53e-19 79.0
MsG0380017440.01.T01 AT2G26695 38.760 129 51 5 5 133 6 106 6.53e-19 79.0

Find 69 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGCGGCGCCGTTGATTT+TGG 0.178200 3:-96277220 MsG0380017440.01.T01:CDS
TTGAGATCACCATACGGTTT+TGG 0.292602 3:-96276998 MsG0380017440.01.T01:CDS
GGGGAGTCAAGAATGATTTC+TGG 0.322815 3:-96277244 MsG0380017440.01.T01:CDS
TTGAGAAGTTCACGATAAAT+AGG 0.332528 3:+96276523 None:intergenic
TCGGTAGCATTCCATTCTAT+TGG 0.379779 3:+96276583 None:intergenic
GGCTCCTTTCTTGGTGGAAG+AGG 0.386495 3:-96277196 MsG0380017440.01.T01:CDS
GAGAAGGTATCAATATCCTT+AGG 0.387497 3:+96277049 None:intergenic
ACTCGCCCCGGCTGGAAATC+CGG 0.405258 3:-96276947 MsG0380017440.01.T01:intron
GTGCCTCCACTCGCCCCGGC+TGG 0.416254 3:-96276955 MsG0380017440.01.T01:CDS
TTATCGTGAACTTCTCAATC+TGG 0.418523 3:-96276518 MsG0380017440.01.T01:CDS
GGGCCGGTGGTGAAAGGAAA+AGG 0.421977 3:+96277163 None:intergenic
CAGTCACCAGGACGGACATC+AGG 0.429559 3:+96277142 None:intergenic
CTGATGTCCGTCCTGGTGAC+TGG 0.431260 3:-96277141 MsG0380017440.01.T01:CDS
AGATGAGCAGACCAGGAGAT+TGG 0.434758 3:-96277324 MsG0380017440.01.T01:CDS
GTCCTGCAGTTCCAATCTCC+TGG 0.444104 3:+96277313 None:intergenic
CTCTTCTTTGAAAGTTGAGG+TGG 0.445847 3:+96277284 None:intergenic
GGCACCACATTTGAAGCAGC+TGG 0.455963 3:+96277072 None:intergenic
GGAGGAGGCAGCGCCGGTGG+TGG 0.457413 3:-96276977 MsG0380017440.01.T01:CDS
TTTGAAGCAGCTGGAGCGGC+TGG 0.457465 3:+96277081 None:intergenic
CGCTCCAGCTGCTTCAAATG+TGG 0.474359 3:-96277076 MsG0380017440.01.T01:CDS
CTTCAAATGTGGTGCCCCTA+AGG 0.478199 3:-96277065 MsG0380017440.01.T01:CDS
CAGTCACCGGATTTCCAGCC+GGG 0.479965 3:+96276941 None:intergenic
AGAGCCTCTTCCACCAAGAA+AGG 0.496788 3:+96277192 None:intergenic
GCATAACTTCGCCAATAGAA+TGG 0.497407 3:-96276594 MsG0380017440.01.T01:CDS
CTTCCAGTACTAGATTCCCT+TGG 0.497781 3:+96276551 None:intergenic
ATTCTCTTCTTTGAAAGTTG+AGG 0.498262 3:+96277281 None:intergenic
TTTGGTGGCTCCTTTCTTGG+TGG 0.500076 3:-96277202 MsG0380017440.01.T01:CDS
AGCAGAAAGATGAGCAGACC+AGG 0.504369 3:-96277331 None:intergenic
CACATTTGAAGCAGCTGGAG+CGG 0.504933 3:+96277077 None:intergenic
GGCCGGTGGTGAAAGGAAAA+GGG 0.509497 3:+96277164 None:intergenic
AGAAGGTATCAATATCCTTA+GGG 0.509984 3:+96277050 None:intergenic
GACCAGGAGATTGGAACTGC+AGG 0.511105 3:-96277315 MsG0380017440.01.T01:CDS
GCTACCGATGCAACGGTCCA+AGG 0.513927 3:-96276568 MsG0380017440.01.T01:CDS
GGATTTCCAGCCGGGGCGAG+TGG 0.514801 3:+96276949 None:intergenic
AGAATGATTTCTGGCGGCGG+CGG 0.516918 3:-96277235 MsG0380017440.01.T01:CDS
GCGAGTGGAGGCACCACCAC+CGG 0.520780 3:+96276964 None:intergenic
GTCTGAGGAGTCAGAGGAGA+AGG 0.523791 3:+96277033 None:intergenic
CCAGTCACCGGATTTCCAGC+CGG 0.530047 3:+96276940 None:intergenic
TTCTTGACTCCCCACATCGT+TGG 0.531867 3:+96277254 None:intergenic
ACCGGCCCTGATGTCCGTCC+TGG 0.532481 3:-96277148 MsG0380017440.01.T01:CDS
GGTCCAAGGGAATCTAGTAC+TGG 0.534715 3:-96276554 MsG0380017440.01.T01:CDS
TTTGGAGGAGGCAGCGCCGG+TGG 0.535377 3:-96276980 MsG0380017440.01.T01:CDS
GAGAATCTTGCCAACGATGT+GGG 0.536610 3:-96277264 MsG0380017440.01.T01:CDS
GGTGACTGGTATTGTACTGT+TGG 0.542639 3:-96277127 MsG0380017440.01.T01:CDS
ACATCAGGGCCGGTGGTGAA+AGG 0.548866 3:+96277157 None:intergenic
ATTCCCTTGGACCGTTGCAT+CGG 0.549976 3:+96276564 None:intergenic
TTTCCAGCCGGGGCGAGTGG+AGG 0.551998 3:+96276952 None:intergenic
CTGCCTCCTCCAAAACCGTA+TGG 0.574316 3:+96276989 None:intergenic
ACCAGGACGGACATCAGGGC+CGG 0.576233 3:+96277147 None:intergenic
AAAGGAGCCACCAAAATCAA+CGG 0.591777 3:+96277210 None:intergenic
CTACCGATGCAACGGTCCAA+GGG 0.596798 3:-96276567 MsG0380017440.01.T01:CDS
GGTGGTGCCTCCACTCGCCC+CGG 0.597153 3:-96276959 MsG0380017440.01.T01:CDS
AGAGAATCTTGCCAACGATG+TGG 0.600788 3:-96277265 MsG0380017440.01.T01:CDS
AGAATCTTGCCAACGATGTG+GGG 0.604150 3:-96277263 MsG0380017440.01.T01:CDS
CAATAATCGTGGCATGTCTG+AGG 0.609735 3:+96277018 None:intergenic
TGGCATGTCTGAGGAGTCAG+AGG 0.612841 3:+96277027 None:intergenic
AGGACGGACATCAGGGCCGG+TGG 0.617823 3:+96277150 None:intergenic
TCAAGAATGATTTCTGGCGG+CGG 0.619694 3:-96277238 MsG0380017440.01.T01:CDS
ATGGAATGCTACCGATGCAA+CGG 0.621720 3:-96276575 MsG0380017440.01.T01:CDS
TATTGTACTGTTGGAAACTG+TGG 0.623871 3:-96277118 MsG0380017440.01.T01:CDS
CGATTATTGAGATCACCATA+CGG 0.625229 3:-96277004 MsG0380017440.01.T01:CDS
AGTCACCAGGACGGACATCA+GGG 0.629819 3:+96277143 None:intergenic
GCCGGTGGTGAAAGGAAAAG+GGG 0.632818 3:+96277165 None:intergenic
ACAGTACAATACCAGTCACC+AGG 0.642353 3:+96277130 None:intergenic
GAGTCAAGAATGATTTCTGG+CGG 0.656995 3:-96277241 MsG0380017440.01.T01:CDS
TACAATACCAGTCACCAGGA+CGG 0.657561 3:+96277134 None:intergenic
TATGGTGATCTCAATAATCG+TGG 0.671531 3:+96277007 None:intergenic
GAAGGTATCAATATCCTTAG+GGG 0.684912 3:+96277051 None:intergenic
AGTCACCGGATTTCCAGCCG+GGG 0.690621 3:+96276942 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTATAAGTTCCAAATT+AGG + Chr3:96276957-96276976 None:intergenic 15.0%
!! CTAACAAAATCTCATAAAAA+TGG - Chr3:96277053-96277072 MsG0380017440.01.T01:CDS 20.0%
!! GATACTTCTTATAAATATTG+TGG - Chr3:96277024-96277043 MsG0380017440.01.T01:CDS 20.0%
!! TTTCATTTGTCTCATTATAA+GGG + Chr3:96277153-96277172 None:intergenic 20.0%
!!! CATTTTTATGAGATTTTGTT+AGG + Chr3:96277055-96277074 None:intergenic 20.0%
! CTTCTTATAAATATTGTGGT+TGG - Chr3:96277028-96277047 MsG0380017440.01.T01:CDS 25.0%
! GTTTCATTTGTCTCATTATA+AGG + Chr3:96277154-96277173 None:intergenic 25.0%
ACATGACACTTATAATGAGA+CGG + Chr3:96276998-96277017 None:intergenic 30.0%
TTGAGAAGTTCACGATAAAT+AGG + Chr3:96277297-96277316 None:intergenic 30.0%
! ATTCTCTTCTTTGAAAGTTG+AGG + Chr3:96276539-96276558 None:intergenic 30.0%
!! AGAAGGTATCAATATCCTTA+GGG + Chr3:96276770-96276789 None:intergenic 30.0%
AGAATTGAAATGTGTAGGTC+TGG - Chr3:96277191-96277210 MsG0380017440.01.T01:CDS 35.0%
CGATTATTGAGATCACCATA+CGG - Chr3:96276813-96276832 MsG0380017440.01.T01:intron 35.0%
GAAATGATTGACATGAAACC+TGG + Chr3:96276910-96276929 None:intergenic 35.0%
GAATTGAAATGTGTAGGTCT+GGG - Chr3:96277192-96277211 MsG0380017440.01.T01:CDS 35.0%
GACCTACACATTTCAATTCT+CGG + Chr3:96277191-96277210 None:intergenic 35.0%
TACCGAGAATTGAAATGTGT+AGG - Chr3:96277186-96277205 MsG0380017440.01.T01:CDS 35.0%
TATGGTGATCTCAATAATCG+TGG + Chr3:96276813-96276832 None:intergenic 35.0%
TATTGTACTGTTGGAAACTG+TGG - Chr3:96276699-96276718 MsG0380017440.01.T01:intron 35.0%
TTATCGTGAACTTCTCAATC+TGG - Chr3:96277299-96277318 MsG0380017440.01.T01:CDS 35.0%
!! GAAGGTATCAATATCCTTAG+GGG + Chr3:96276769-96276788 None:intergenic 35.0%
!! GAGAAGGTATCAATATCCTT+AGG + Chr3:96276771-96276790 None:intergenic 35.0%
AAAGGAGCCACCAAAATCAA+CGG + Chr3:96276610-96276629 None:intergenic 40.0%
CAAATTAGGACGAAGTAGCA+AGG + Chr3:96276943-96276962 None:intergenic 40.0%
CAATTCTCGGTAATGTCAGT+AGG + Chr3:96277178-96277197 None:intergenic 40.0%
GCATAACTTCGCCAATAGAA+TGG - Chr3:96277223-96277242 MsG0380017440.01.T01:CDS 40.0%
TCGGTAGCATTCCATTCTAT+TGG + Chr3:96277237-96277256 None:intergenic 40.0%
! CTCTTCTTTGAAAGTTGAGG+TGG + Chr3:96276536-96276555 None:intergenic 40.0%
! CTTGCTACTTCGTCCTAATT+TGG - Chr3:96276941-96276960 MsG0380017440.01.T01:intron 40.0%
! GAGTCAAGAATGATTTCTGG+CGG - Chr3:96276576-96276595 MsG0380017440.01.T01:CDS 40.0%
! TTGAGATCACCATACGGTTT+TGG - Chr3:96276819-96276838 MsG0380017440.01.T01:intron 40.0%
ACAGTACAATACCAGTCACC+AGG + Chr3:96276690-96276709 None:intergenic 45.0%
AGAATCTTGCCAACGATGTG+GGG - Chr3:96276554-96276573 MsG0380017440.01.T01:CDS 45.0%
AGAGAATCTTGCCAACGATG+TGG - Chr3:96276552-96276571 MsG0380017440.01.T01:CDS 45.0%
ATGGAATGCTACCGATGCAA+CGG - Chr3:96277242-96277261 MsG0380017440.01.T01:CDS 45.0%
CAATAATCGTGGCATGTCTG+AGG + Chr3:96276802-96276821 None:intergenic 45.0%
CTTCCAGTACTAGATTCCCT+TGG + Chr3:96277269-96277288 None:intergenic 45.0%
GAGAATCTTGCCAACGATGT+GGG - Chr3:96276553-96276572 MsG0380017440.01.T01:CDS 45.0%
GGGGAGTCAAGAATGATTTC+TGG - Chr3:96276573-96276592 MsG0380017440.01.T01:CDS 45.0%
GGTGACTGGTATTGTACTGT+TGG - Chr3:96276690-96276709 MsG0380017440.01.T01:intron 45.0%
TACAATACCAGTCACCAGGA+CGG + Chr3:96276686-96276705 None:intergenic 45.0%
!! AGATCACCATACGGTTTTGG+AGG - Chr3:96276822-96276841 MsG0380017440.01.T01:intron 45.0%
!! GATTTTGGTGGCTCCTTTCT+TGG - Chr3:96276612-96276631 MsG0380017440.01.T01:CDS 45.0%
!! TCAAGAATGATTTCTGGCGG+CGG - Chr3:96276579-96276598 MsG0380017440.01.T01:CDS 45.0%
AGAGCCTCTTCCACCAAGAA+AGG + Chr3:96276628-96276647 None:intergenic 50.0%
ATTCCCTTGGACCGTTGCAT+CGG + Chr3:96277256-96277275 None:intergenic 50.0%
CTTCAAATGTGGTGCCCCTA+AGG - Chr3:96276752-96276771 MsG0380017440.01.T01:intron 50.0%
GGTCCAAGGGAATCTAGTAC+TGG - Chr3:96277263-96277282 MsG0380017440.01.T01:CDS 50.0%
TTCTTGACTCCCCACATCGT+TGG + Chr3:96276566-96276585 None:intergenic 50.0%
! CACATTTGAAGCAGCTGGAG+CGG + Chr3:96276743-96276762 None:intergenic 50.0%
! TCCCCTTTTCCTTTCACCAC+CGG - Chr3:96276651-96276670 MsG0380017440.01.T01:intron 50.0%
! TTTGGTGGCTCCTTTCTTGG+TGG - Chr3:96276615-96276634 MsG0380017440.01.T01:intron 50.0%
!!! TCACCATACGGTTTTGGAGG+AGG - Chr3:96276825-96276844 MsG0380017440.01.T01:intron 50.0%
AGTCACCAGGACGGACATCA+GGG + Chr3:96276677-96276696 None:intergenic 55.0%
CCTGGTGCATATCCAGTCAC+CGG + Chr3:96276892-96276911 None:intergenic 55.0%
CGCTCCAGCTGCTTCAAATG+TGG - Chr3:96276741-96276760 MsG0380017440.01.T01:intron 55.0%
CTACCGATGCAACGGTCCAA+GGG - Chr3:96277250-96277269 MsG0380017440.01.T01:CDS 55.0%
CTGCCTCCTCCAAAACCGTA+TGG + Chr3:96276831-96276850 None:intergenic 55.0%
GACCAGGAGATTGGAACTGC+AGG - Chr3:96276502-96276521 MsG0380017440.01.T01:CDS 55.0%
GCCGGTGGTGAAAGGAAAAG+GGG + Chr3:96276655-96276674 None:intergenic 55.0%
GGCCGGTGGTGAAAGGAAAA+GGG + Chr3:96276656-96276675 None:intergenic 55.0%
GGCTCCTTTCTTGGTGGAAG+AGG - Chr3:96276621-96276640 MsG0380017440.01.T01:intron 55.0%
GTCCTGCAGTTCCAATCTCC+TGG + Chr3:96276507-96276526 None:intergenic 55.0%
GTCTGAGGAGTCAGAGGAGA+AGG + Chr3:96276787-96276806 None:intergenic 55.0%
TGGCATGTCTGAGGAGTCAG+AGG + Chr3:96276793-96276812 None:intergenic 55.0%
! GGCACCACATTTGAAGCAGC+TGG + Chr3:96276748-96276767 None:intergenic 55.0%
!! AGAATGATTTCTGGCGGCGG+CGG - Chr3:96276582-96276601 MsG0380017440.01.T01:CDS 55.0%
ACATCAGGGCCGGTGGTGAA+AGG + Chr3:96276663-96276682 None:intergenic 60.0%
AGTCACCGGATTTCCAGCCG+GGG + Chr3:96276878-96276897 None:intergenic 60.0%
CAGTCACCAGGACGGACATC+AGG + Chr3:96276678-96276697 None:intergenic 60.0%
CAGTCACCGGATTTCCAGCC+GGG + Chr3:96276879-96276898 None:intergenic 60.0%
CCAGTCACCGGATTTCCAGC+CGG + Chr3:96276880-96276899 None:intergenic 60.0%
CCGGTGACTGGATATGCACC+AGG - Chr3:96276889-96276908 MsG0380017440.01.T01:intron 60.0%
CTGATGTCCGTCCTGGTGAC+TGG - Chr3:96276676-96276695 MsG0380017440.01.T01:intron 60.0%
GCTACCGATGCAACGGTCCA+AGG - Chr3:96277249-96277268 MsG0380017440.01.T01:CDS 60.0%
GGGCCGGTGGTGAAAGGAAA+AGG + Chr3:96276657-96276676 None:intergenic 60.0%
! TTTGAAGCAGCTGGAGCGGC+TGG + Chr3:96276739-96276758 None:intergenic 60.0%
ACCAGGACGGACATCAGGGC+CGG + Chr3:96276673-96276692 None:intergenic 65.0%
ACTCGCCCCGGCTGGAAATC+CGG - Chr3:96276870-96276889 MsG0380017440.01.T01:intron 65.0%
CCGGCTGGAAATCCGGTGAC+TGG - Chr3:96276877-96276896 MsG0380017440.01.T01:intron 65.0%
!!! GGCGGCGCCGTTGATTTTGG+TGG - Chr3:96276600-96276619 MsG0380017440.01.T01:CDS 65.0%
!!! GGTTTTGGAGGAGGCAGCGC+CGG - Chr3:96276834-96276853 MsG0380017440.01.T01:intron 65.0%
ACCGGCCCTGATGTCCGTCC+TGG - Chr3:96276669-96276688 MsG0380017440.01.T01:intron 70.0%
AGGACGGACATCAGGGCCGG+TGG + Chr3:96276670-96276689 None:intergenic 70.0%
GGATTTCCAGCCGGGGCGAG+TGG + Chr3:96276871-96276890 None:intergenic 70.0%
TTTCCAGCCGGGGCGAGTGG+AGG + Chr3:96276868-96276887 None:intergenic 70.0%
! TTTGGAGGAGGCAGCGCCGG+TGG - Chr3:96276837-96276856 MsG0380017440.01.T01:intron 70.0%
!! GCGAGTGGAGGCACCACCAC+CGG + Chr3:96276856-96276875 None:intergenic 70.0%
!!! GGCGGCGGCGCCGTTGATTT+TGG - Chr3:96276597-96276616 MsG0380017440.01.T01:CDS 70.0%
GGTGGTGCCTCCACTCGCCC+CGG - Chr3:96276858-96276877 MsG0380017440.01.T01:intron 75.0%
! GTGCCTCCACTCGCCCCGGC+TGG - Chr3:96276862-96276881 MsG0380017440.01.T01:intron 80.0%
!!! GGAGGAGGCAGCGCCGGTGG+TGG - Chr3:96276840-96276859 MsG0380017440.01.T01:intron 80.0%
Chromosome Type Strat End Strand Name
Chr3 gene 96276495 96277344 96276495 ID=MsG0380017440.01;Name=MsG0380017440.01
Chr3 mRNA 96276495 96277344 96276495 ID=MsG0380017440.01.T01;Parent=MsG0380017440.01;Name=MsG0380017440.01.T01;_AED=0.50;_eAED=0.63;_QI=0|0|0|1|1|1|2|0|177
Chr3 exon 96276948 96277344 96276948 ID=MsG0380017440.01.T01:exon:17494;Parent=MsG0380017440.01.T01
Chr3 exon 96276495 96276631 96276495 ID=MsG0380017440.01.T01:exon:17493;Parent=MsG0380017440.01.T01
Chr3 CDS 96276948 96277344 96276948 ID=MsG0380017440.01.T01:cds;Parent=MsG0380017440.01.T01
Chr3 CDS 96276495 96276631 96276495 ID=MsG0380017440.01.T01:cds;Parent=MsG0380017440.01.T01
Gene Sequence

>MsG0380017440.01.T01

ATGAGCAGACCAGGAGATTGGAACTGCAGGACATGCAACCACCTCAACTTTCAAAGAAGAGAATCTTGCCAACGATGTGGGGAGTCAAGAATGATTTCTGGCGGCGGCGGCGCCGTTGATTTTGGTGGCTCCTTTCTTGGTGGAAGAGGCTCTAGCTCCCCTTTTCCTTTCACCACCGGCCCTGATGTCCGTCCTGGTGACTGGTATTGTACTGTTGGAAACTGTGGAGCTCACAACTTTGCCAGCCGCTCCAGCTGCTTCAAATGTGGTGCCCCTAAGGATATTGATACCTTCTCCTCTGACTCCTCAGACATGCCACGATTATTGAGATCACCATACGGTTTTGGAGGAGGCAGCGCCGGTGGTGGTGCCTCCACTCGCCCCGGCTGGAAATCCGGTCTGGGTGTAACGAGCATAACTTCGCCAATAGAATGGAATGCTACCGATGCAACGGTCCAAGGGAATCTAGTACTGGAAGATCTTCCTATTTATCGTGAACTTCTCAATCTGGTAATTCATTCTGATATCATTTAA

Protein sequence

>MsG0380017440.01.T01

MSRPGDWNCRTCNHLNFQRRESCQRCGESRMISGGGGAVDFGGSFLGGRGSSSPFPFTTGPDVRPGDWYCTVGNCGAHNFASRSSCFKCGAPKDIDTFSSDSSDMPRLLRSPYGFGGGSAGGGASTRPGWKSGLGVTSITSPIEWNATDATVQGNLVLEDLPIYRELLNLVIHSDII*