AlfalfaGEDB | MsG0380017440.01

M. sativa cultivar ZhongmuNo.1 / MsG0380017440.01

KEGG: -
GO: -
PFAM: PF00641

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017440.01.T01	PNY09071.1	83.562	146	19	2	1	146	1	141	9.39E-79	241

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017440.01.T01	A0A072V277	96.992	133	3	1	1	133	1	132	8.02e-77	236

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0380017440.01	0.859760	3.308713e-63	8.836174e-60
MsG0080048258.01	MsG0380017440.01	0.825194	5.354152e-54	4.846712e-51
MsG0180000111.01	MsG0380017440.01	0.845041	5.200991e-59	8.558446e-56
MsG0180000817.01	MsG0380017440.01	0.803537	3.325621e-49	1.675513e-46
MsG0180002211.01	MsG0380017440.01	0.812826	3.486564e-51	2.239420e-48
MsG0180002583.01	MsG0380017440.01	0.802240	6.162580e-49	3.003534e-46
MsG0180003360.01	MsG0380017440.01	0.800043	1.733840e-48	7.992690e-46
MsG0180003485.01	MsG0380017440.01	0.878937	1.874465e-69	9.971771e-66
MsG0180004471.01	MsG0380017440.01	0.802354	5.837110e-49	2.853346e-46
MsG0180004538.01	MsG0380017440.01	0.813387	2.626563e-51	1.712947e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017440.01.T01	MTR_3g107610	96.992	133	3	1	1	133	1	132	2.03e-80	236
MsG0380017440.01.T01	MTR_3g107610	89.726	146	14	1	1	146	1	145	4.00e-80	235
MsG0380017440.01.T01	MTR_4g011190	59.398	133	38	4	1	133	1	117	4.41e-37	125
MsG0380017440.01.T01	MTR_4g113840	39.416	137	40	6	5	133	6	107	2.43e-18	77.4
MsG0380017440.01.T01	MTR_2g054750	36.893	103	46	2	5	106	6	90	9.27e-18	75.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380017440.01.T01	AT3G15680	74.468	94	21	2	1	94	1	91	3.93e-42	139
MsG0380017440.01.T01	AT3G15680	74.468	94	21	2	1	94	1	91	3.93e-42	139
MsG0380017440.01.T01	AT5G25490	65.049	103	32	2	1	103	1	99	8.36e-38	128
MsG0380017440.01.T01	AT5G25490	65.049	103	32	2	1	103	1	99	8.36e-38	128
MsG0380017440.01.T01	AT2G26695	38.760	129	51	5	5	133	6	106	6.53e-19	79.0
MsG0380017440.01.T01	AT2G26695	38.760	129	51	5	5	133	6	106	6.53e-19	79.0

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Find 69 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAGCCACCAAAATCAA+CGG	0.591777	3:+96277210	None:intergenic
ACAGTACAATACCAGTCACC+AGG	0.642353	3:+96277130	None:intergenic
ACATCAGGGCCGGTGGTGAA+AGG	0.548866	3:+96277157	None:intergenic
ACCAGGACGGACATCAGGGC+CGG	0.576233	3:+96277147	None:intergenic
ACCGGCCCTGATGTCCGTCC+TGG	0.532481	3:-96277148	MsG0380017440.01.T01:CDS
ACTCGCCCCGGCTGGAAATC+CGG	0.405258	3:-96276947	MsG0380017440.01.T01:intron
AGAAGGTATCAATATCCTTA+GGG	0.509984	3:+96277050	None:intergenic
AGAATCTTGCCAACGATGTG+GGG	0.604150	3:-96277263	MsG0380017440.01.T01:CDS
AGAATGATTTCTGGCGGCGG+CGG	0.516918	3:-96277235	MsG0380017440.01.T01:CDS
AGAGAATCTTGCCAACGATG+TGG	0.600788	3:-96277265	MsG0380017440.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATGACACTTATAATGAGA+CGG	+	Chr3:96276998-96277017	None:intergenic	30.0%
TTGAGAAGTTCACGATAAAT+AGG	+	Chr3:96277297-96277316	None:intergenic	30.0%
AGAATTGAAATGTGTAGGTC+TGG	-	Chr3:96277191-96277210	MsG0380017440.01.T01:CDS	35.0%
CGATTATTGAGATCACCATA+CGG	-	Chr3:96276813-96276832	MsG0380017440.01.T01:intron	35.0%
GAAATGATTGACATGAAACC+TGG	+	Chr3:96276910-96276929	None:intergenic	35.0%
GAATTGAAATGTGTAGGTCT+GGG	-	Chr3:96277192-96277211	MsG0380017440.01.T01:CDS	35.0%
GACCTACACATTTCAATTCT+CGG	+	Chr3:96277191-96277210	None:intergenic	35.0%
TACCGAGAATTGAAATGTGT+AGG	-	Chr3:96277186-96277205	MsG0380017440.01.T01:CDS	35.0%
TATGGTGATCTCAATAATCG+TGG	+	Chr3:96276813-96276832	None:intergenic	35.0%
TATTGTACTGTTGGAAACTG+TGG	-	Chr3:96276699-96276718	MsG0380017440.01.T01:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	96276495	96277344	96276495	ID=MsG0380017440.01;Name=MsG0380017440.01
Chr3	mRNA	96276495	96277344	96276495	ID=MsG0380017440.01.T01;Parent=MsG0380017440.01;Name=MsG0380017440.01.T01;_AED=0.50;_eAED=0.63;_QI=0\|0\|0\|1\|1\|1\|2\|0\|177
Chr3	exon	96276948	96277344	96276948	ID=MsG0380017440.01.T01:exon:17494;Parent=MsG0380017440.01.T01
Chr3	exon	96276495	96276631	96276495	ID=MsG0380017440.01.T01:exon:17493;Parent=MsG0380017440.01.T01
Chr3	CDS	96276948	96277344	96276948	ID=MsG0380017440.01.T01:cds;Parent=MsG0380017440.01.T01
Chr3	CDS	96276495	96276631	96276495	ID=MsG0380017440.01.T01:cds;Parent=MsG0380017440.01.T01

Gene Sequence

>MsG0380017440.01.T01

ATGAGCAGACCAGGAGATTGGAACTGCAGGACATGCAACCACCTCAACTTTCAAAGAAGAGAATCTTGCCAACGATGTGGGGAGTCAAGAATGATTTCTGGCGGCGGCGGCGCCGTTGATTTTGGTGGCTCCTTTCTTGGTGGAAGAGGCTCTAGCTCCCCTTTTCCTTTCACCACCGGCCCTGATGTCCGTCCTGGTGACTGGTATTGTACTGTTGGAAACTGTGGAGCTCACAACTTTGCCAGCCGCTCCAGCTGCTTCAAATGTGGTGCCCCTAAGGATATTGATACCTTCTCCTCTGACTCCTCAGACATGCCACGATTATTGAGATCACCATACGGTTTTGGAGGAGGCAGCGCCGGTGGTGGTGCCTCCACTCGCCCCGGCTGGAAATCCGGTCTGGGTGTAACGAGCATAACTTCGCCAATAGAATGGAATGCTACCGATGCAACGGTCCAAGGGAATCTAGTACTGGAAGATCTTCCTATTTATCGTGAACTTCTCAATCTGGTAATTCATTCTGATATCATTTAA

Protein sequence

>MsG0380017440.01.T01

MSRPGDWNCRTCNHLNFQRRESCQRCGESRMISGGGGAVDFGGSFLGGRGSSSPFPFTTGPDVRPGDWYCTVGNCGAHNFASRSSCFKCGAPKDIDTFSSDSSDMPRLLRSPYGFGGGSAGGGASTRPGWKSGLGVTSITSPIEWNATDATVQGNLVLEDLPIYRELLNLVIHSDII*