AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023867.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023867.01.T01 MTR_8g103890 96.939 98 3 0 33 130 146 243 1.90e-64 198
MsG0480023867.01.T01 MTR_8g085910 43.697 119 61 1 33 151 99 211 7.92e-30 108
MsG0480023867.01.T01 MTR_8g095500 51.064 94 46 0 36 129 133 226 1.81e-29 108
MsG0480023867.01.T01 MTR_7g082240 50.000 94 47 0 36 129 141 234 4.40e-29 107
MsG0480023867.01.T01 MTR_8g103890 94.118 51 3 0 33 83 146 196 1.86e-27 102
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023867.01.T01 AT1G18440 71.429 98 28 0 33 130 176 273 2.23e-46 153
MsG0480023867.01.T01 AT5G19830 46.789 109 58 0 33 141 100 208 3.07e-31 112
MsG0480023867.01.T01 AT5G16140 50.526 95 47 0 36 130 130 224 3.27e-28 105
MsG0480023867.01.T01 AT5G16140 50.526 95 47 0 36 130 130 224 3.27e-28 105
MsG0480023867.01.T01 AT5G38290 46.000 100 54 0 30 129 134 233 1.91e-26 100
MsG0480023867.01.T01 AT5G38290 46.000 100 54 0 30 129 130 229 2.06e-26 100

Find 50 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGACAATTATATATAAAA+AGG 0.203987 4:-90686370 MsG0480023867.01.T01:exon
GATGATAAAAGGAGAATATT+TGG 0.243324 4:-90686019 MsG0480023867.01.T01:three_prime_UTR
ATGTATTAGGTCCAAGTATT+AGG 0.269682 4:+90686201 None:intergenic
ATTCTTTCAGAAAGCAGTTT+AGG 0.299345 4:-90686245 MsG0480023867.01.T01:three_prime_UTR
AATCACTTGAAAGGGAATTC+TGG 0.305312 4:-90686814 MsG0480023867.01.T01:CDS
AAAATAATAATGATGATAAA+AGG 0.308316 4:-90686030 MsG0480023867.01.T01:three_prime_UTR
AGTTATGGAATTATTAGTTC+TGG 0.353013 4:-90686125 MsG0480023867.01.T01:three_prime_UTR
GAAACACAACCATATGTATT+AGG 0.368693 4:+90686188 None:intergenic
TCTTCGACCTTTCACTAAAC+AGG 0.373536 4:-90686631 MsG0480023867.01.T01:CDS
TCAATCAAAAGACATGTGTT+TGG 0.391409 4:-90686289 MsG0480023867.01.T01:three_prime_UTR
AATCTTGTAATATGAAACTA+TGG 0.393893 4:+90687168 None:intergenic
TGACCTCCATGTCCACCCTT+TGG 0.407317 4:+90686977 None:intergenic
TTTCATTAGTTGAGTGATTA+CGG 0.423303 4:-90686169 MsG0480023867.01.T01:three_prime_UTR
TTAGGCATTGGACGACCTCC+TGG 0.434728 4:-90686678 MsG0480023867.01.T01:intron
ACCTTTCACTAAACAGGAAA+AGG 0.440571 4:-90686625 MsG0480023867.01.T01:CDS
TACCACAAGTACTGCTTCAA+TGG 0.444212 4:+90687145 None:intergenic
TAAACTGCTTTCTGAAAGAA+TGG 0.448242 4:+90686247 None:intergenic
TTCTCATTCTTTCGGTCGAA+AGG 0.451027 4:-90686082 MsG0480023867.01.T01:three_prime_UTR
ATACTTGGACCTAATACATA+TGG 0.467321 4:-90686197 MsG0480023867.01.T01:three_prime_UTR
TGGACGACCTCCTGGGAAGA+TGG 0.477400 4:-90686670 MsG0480023867.01.T01:CDS
AGAAGCCGTAATTTAGCAAA+AGG 0.494628 4:+90687001 None:intergenic
GGCTTCTAATCCATCTTGTA+AGG 0.495870 4:+90686495 None:intergenic
AGTGTTATTAATCACTTGAA+AGG 0.508112 4:-90686823 MsG0480023867.01.T01:CDS
ATCAAAAGACATGTGTTTGG+AGG 0.509196 4:-90686286 MsG0480023867.01.T01:three_prime_UTR
GTGGACATGGAGGTCACAAT+GGG 0.517509 4:-90686970 MsG0480023867.01.T01:intron
CACTAAACAGGAAAAGGAAG+AGG 0.528639 4:-90686619 MsG0480023867.01.T01:intron
CTTCTACCAAAGGGTGGACA+TGG 0.529468 4:-90686983 MsG0480023867.01.T01:CDS
GTGTTATTAATCACTTGAAA+GGG 0.530399 4:-90686822 MsG0480023867.01.T01:CDS
GAATTGTAACATGATAGTTA+TGG 0.531143 4:-90686140 MsG0480023867.01.T01:three_prime_UTR
GGTGGACATGGAGGTCACAA+TGG 0.533801 4:-90686971 MsG0480023867.01.T01:CDS
TATTAGTTCTGGTTCTGTTG+AGG 0.536881 4:-90686114 MsG0480023867.01.T01:three_prime_UTR
GTGAATTCGAACCTAATACT+TGG 0.544003 4:-90686212 MsG0480023867.01.T01:three_prime_UTR
TGTGTTACTAACTGGATACA+AGG 0.544580 4:+90687289 None:intergenic
TAAATTACGGCTTCTACCAA+AGG 0.548372 4:-90686993 MsG0480023867.01.T01:CDS
GCCACAGGATCCATCTTCCC+AGG 0.554752 4:+90686660 None:intergenic
AAATTACGGCTTCTACCAAA+GGG 0.576959 4:-90686992 MsG0480023867.01.T01:CDS
TCCTGGGAAGATGGATCCTG+TGG 0.578652 4:-90686661 MsG0480023867.01.T01:CDS
TTCCATTGAAGCAGTACTTG+TGG 0.581943 4:-90687147 MsG0480023867.01.T01:intron
TAGGCATTGGACGACCTCCT+GGG 0.598024 4:-90686677 MsG0480023867.01.T01:CDS
GTGTTACTAACTGGATACAA+GGG 0.602294 4:+90687290 None:intergenic
GGTCGAAGAACAAATGCCAC+AGG 0.607460 4:+90686645 None:intergenic
AACTTACCAATTCTTAAGCG+AGG 0.614139 4:+90686787 None:intergenic
AAAGATGGAGCAAACAGCTG+AGG 0.646414 4:-90686414 MsG0480023867.01.T01:CDS
TGTTACTAACTGGATACAAG+GGG 0.647027 4:+90687291 None:intergenic
GTCCAACAAAATAATCCGCA+CGG 0.667338 4:+90686474 None:intergenic
CTACCAAAGGGTGGACATGG+AGG 0.668180 4:-90686980 MsG0480023867.01.T01:CDS
TTACGGCTTCTACCAAAGGG+TGG 0.677654 4:-90686989 MsG0480023867.01.T01:CDS
ACAGGATCCATCTTCCCAGG+AGG 0.722210 4:+90686663 None:intergenic
AAGATGGATTAGAAGCCGTG+CGG 0.769873 4:-90686489 MsG0480023867.01.T01:CDS
GTTACTAACTGGATACAAGG+GGG 0.783917 4:+90687292 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATAATGATGATAAA+AGG - Chr4:90687325-90687344 MsG0480023867.01.T01:CDS 10.0%
!! TATATAATTATCATCATTTA+GGG + Chr4:90686819-90686838 None:intergenic 10.0%
!! TTATATAATTATCATCATTT+AGG + Chr4:90686820-90686839 None:intergenic 10.0%
!! TTTGACAATTATATATAAAA+AGG - Chr4:90686985-90687004 MsG0480023867.01.T01:CDS 10.0%
!!! TTTTATATATAATTGTCAAA+AGG + Chr4:90686985-90687004 None:intergenic 10.0%
!! ATTTATATAGTAAATTAGCA+TGG + Chr4:90686113-90686132 None:intergenic 15.0%
!! AATCTTGTAATATGAAACTA+TGG + Chr4:90686190-90686209 None:intergenic 20.0%
!!! ATTCATTTTTCTCATTCTTT+CGG - Chr4:90687265-90687284 MsG0480023867.01.T01:intron 20.0%
!!! CATTTATTGTTGATTTTGTT+AGG - Chr4:90686659-90686678 MsG0480023867.01.T01:CDS 20.0%
! AGTGATGAAGAAAAATTAGA+AGG - Chr4:90686266-90686285 MsG0480023867.01.T01:three_prime_UTR 25.0%
! AGTTATGGAATTATTAGTTC+TGG - Chr4:90687230-90687249 MsG0480023867.01.T01:intron 25.0%
! ATGCTTAAATATAACTCGAT+AGG + Chr4:90686482-90686501 None:intergenic 25.0%
! GAATTGTAACATGATAGTTA+TGG - Chr4:90687215-90687234 MsG0480023867.01.T01:intron 25.0%
! GATGATAAAAGGAGAATATT+TGG - Chr4:90687336-90687355 MsG0480023867.01.T01:CDS 25.0%
! TTTCATTAGTTGAGTGATTA+CGG - Chr4:90687186-90687205 MsG0480023867.01.T01:intron 25.0%
!! AGTGTTATTAATCACTTGAA+AGG - Chr4:90686532-90686551 MsG0480023867.01.T01:intron 25.0%
!! ATTTGCCTTTTGCTAAATTA+CGG - Chr4:90686349-90686368 MsG0480023867.01.T01:three_prime_UTR 25.0%
!! GTGTTATTAATCACTTGAAA+GGG - Chr4:90686533-90686552 MsG0480023867.01.T01:intron 25.0%
!! TGTATAAAAAAGTGTACAAG+AGG + Chr4:90687299-90687318 None:intergenic 25.0%
!! TTCTTTTTGAACCAACAAAT+TGG - Chr4:90686775-90686794 MsG0480023867.01.T01:intron 25.0%
AATTAGAAGGATTATCTGCT+TGG - Chr4:90686279-90686298 MsG0480023867.01.T01:three_prime_UTR 30.0%
ATACTTGGACCTAATACATA+TGG - Chr4:90687158-90687177 MsG0480023867.01.T01:CDS 30.0%
ATGTATTAGGTCCAAGTATT+AGG + Chr4:90687157-90687176 None:intergenic 30.0%
ATTCTTTCAGAAAGCAGTTT+AGG - Chr4:90687110-90687129 MsG0480023867.01.T01:intron 30.0%
GAAACACAACCATATGTATT+AGG + Chr4:90687170-90687189 None:intergenic 30.0%
TCAATCAAAAGACATGTGTT+TGG - Chr4:90687066-90687085 MsG0480023867.01.T01:intron 30.0%
TGTTAATAGTGCCAAAAAGA+TGG - Chr4:90686926-90686945 MsG0480023867.01.T01:intron 30.0%
! CTGAATTTTACCTTACAAGA+TGG - Chr4:90686850-90686869 MsG0480023867.01.T01:intron 30.0%
! TAAACTGCTTTCTGAAAGAA+TGG + Chr4:90687111-90687130 None:intergenic 30.0%
!! ACACATGTCTTTTGATTGAA+TGG + Chr4:90687065-90687084 None:intergenic 30.0%
!!! ATATTTTAATGCTGAGTTGC+TGG - Chr4:90686137-90686156 MsG0480023867.01.T01:three_prime_UTR 30.0%
!!! TTGTTGATTTTGTTAGGCAT+TGG - Chr4:90686665-90686684 MsG0480023867.01.T01:CDS 30.0%
AAATTACGGCTTCTACCAAA+GGG - Chr4:90686363-90686382 MsG0480023867.01.T01:exon 35.0%
AACTTACCAATTCTTAAGCG+AGG + Chr4:90686571-90686590 None:intergenic 35.0%
AATAACACTCTTCATTCTGC+AGG + Chr4:90686521-90686540 None:intergenic 35.0%
AATCACTTGAAAGGGAATTC+TGG - Chr4:90686541-90686560 MsG0480023867.01.T01:intron 35.0%
ACCTTTCACTAAACAGGAAA+AGG - Chr4:90686730-90686749 MsG0480023867.01.T01:intron 35.0%
AGAAGCCGTAATTTAGCAAA+AGG + Chr4:90686357-90686376 None:intergenic 35.0%
ATAACACTCTTCATTCTGCA+GGG + Chr4:90686520-90686539 None:intergenic 35.0%
ATCAAAAGACATGTGTTTGG+AGG - Chr4:90687069-90687088 MsG0480023867.01.T01:intron 35.0%
GTACCTAAAAGCCAATTTGT+TGG + Chr4:90686789-90686808 None:intergenic 35.0%
GTGAATTCGAACCTAATACT+TGG - Chr4:90687143-90687162 MsG0480023867.01.T01:intron 35.0%
TAAATTACGGCTTCTACCAA+AGG - Chr4:90686362-90686381 MsG0480023867.01.T01:exon 35.0%
TGTTACTAACTGGATACAAG+GGG + Chr4:90686067-90686086 None:intergenic 35.0%
! CAAGGATATGTGTTACTAAC+TGG + Chr4:90686077-90686096 None:intergenic 35.0%
! GTGTTACTAACTGGATACAA+GGG + Chr4:90686068-90686087 None:intergenic 35.0%
! TCCTTTTCCTGTTTAGTGAA+AGG + Chr4:90686734-90686753 None:intergenic 35.0%
! TGTGTTACTAACTGGATACA+AGG + Chr4:90686069-90686088 None:intergenic 35.0%
!! CATGTCTTTTGATTGAATGG+AGG + Chr4:90687062-90687081 None:intergenic 35.0%
!! TATTAGTTCTGGTTCTGTTG+AGG - Chr4:90687241-90687260 MsG0480023867.01.T01:intron 35.0%
!!! CAAATTGGCTTTTAGGTACA+TGG - Chr4:90686790-90686809 MsG0480023867.01.T01:intron 35.0%
!!! GAACCAACAAATTGGCTTTT+AGG - Chr4:90686783-90686802 MsG0480023867.01.T01:intron 35.0%
!!! TTGGAATCTGTTTTGAAGGT+TGG - Chr4:90686162-90686181 MsG0480023867.01.T01:three_prime_UTR 35.0%
AACCCAAGTAAATGCTGCAA+TGG + Chr4:90686448-90686467 None:intergenic 40.0%
CACTAAACAGGAAAAGGAAG+AGG - Chr4:90686736-90686755 MsG0480023867.01.T01:intron 40.0%
CATGGATACAACAAACGACA+AGG + Chr4:90686095-90686114 None:intergenic 40.0%
CTCCATTGCAGCATTTACTT+GGG - Chr4:90686443-90686462 MsG0480023867.01.T01:CDS 40.0%
GGCTTCTAATCCATCTTGTA+AGG + Chr4:90686863-90686882 None:intergenic 40.0%
GTCCAACAAAATAATCCGCA+CGG + Chr4:90686884-90686903 None:intergenic 40.0%
GTGAGTAAGTGACTGTAACA+TGG + Chr4:90686623-90686642 None:intergenic 40.0%
GTTACTAACTGGATACAAGG+GGG + Chr4:90686066-90686085 None:intergenic 40.0%
TCTTCGACCTTTCACTAAAC+AGG - Chr4:90686724-90686743 MsG0480023867.01.T01:intron 40.0%
TTCCATTGAAGCAGTACTTG+TGG - Chr4:90686208-90686227 MsG0480023867.01.T01:three_prime_UTR 40.0%
TTCTCATTCTTTCGGTCGAA+AGG - Chr4:90687273-90687292 MsG0480023867.01.T01:intron 40.0%
TTGCAGCATTTACTTGGGTT+TGG - Chr4:90686448-90686467 MsG0480023867.01.T01:CDS 40.0%
! AGCTGTTTGCTCCATCTTTT+TGG + Chr4:90686940-90686959 None:intergenic 40.0%
! GGTTTTCCTCGCTTAAGAAT+TGG - Chr4:90686562-90686581 MsG0480023867.01.T01:intron 40.0%
! TACCACAAGTACTGCTTCAA+TGG + Chr4:90686213-90686232 None:intergenic 40.0%
!! GGTGTTGGAATCTGTTTTGA+AGG - Chr4:90686158-90686177 MsG0480023867.01.T01:three_prime_UTR 40.0%
!! GTGCGGATTATTTTGTTGGA+CGG - Chr4:90686883-90686902 MsG0480023867.01.T01:intron 40.0%
AAAGATGGAGCAAACAGCTG+AGG - Chr4:90686941-90686960 MsG0480023867.01.T01:intron 45.0%
GCTCCATTGCAGCATTTACT+TGG - Chr4:90686442-90686461 MsG0480023867.01.T01:CDS 45.0%
TAATGCTGAGTTGCTGGTGT+TGG - Chr4:90686143-90686162 MsG0480023867.01.T01:three_prime_UTR 45.0%
!! AAGATGGATTAGAAGCCGTG+CGG - Chr4:90686866-90686885 MsG0480023867.01.T01:intron 45.0%
!! AGCCGTGCGGATTATTTTGT+TGG - Chr4:90686879-90686898 MsG0480023867.01.T01:intron 45.0%
CTTCTACCAAAGGGTGGACA+TGG - Chr4:90686372-90686391 MsG0480023867.01.T01:exon 50.0%
GGTCGAAGAACAAATGCCAC+AGG + Chr4:90686713-90686732 None:intergenic 50.0%
GTGGACATGGAGGTCACAAT+GGG - Chr4:90686385-90686404 MsG0480023867.01.T01:CDS 50.0%
TTACGGCTTCTACCAAAGGG+TGG - Chr4:90686366-90686385 MsG0480023867.01.T01:exon 50.0%
ACAGGATCCATCTTCCCAGG+AGG + Chr4:90686695-90686714 None:intergenic 55.0%
CTACCAAAGGGTGGACATGG+AGG - Chr4:90686375-90686394 MsG0480023867.01.T01:CDS 55.0%
GGTGGACATGGAGGTCACAA+TGG - Chr4:90686384-90686403 MsG0480023867.01.T01:CDS 55.0%
TCCTGGGAAGATGGATCCTG+TGG - Chr4:90686694-90686713 MsG0480023867.01.T01:intron 55.0%
TGACCTCCATGTCCACCCTT+TGG + Chr4:90686381-90686400 None:intergenic 55.0%
!! TAGGCATTGGACGACCTCCT+GGG - Chr4:90686678-90686697 MsG0480023867.01.T01:intron 55.0%
!! TTAGGCATTGGACGACCTCC+TGG - Chr4:90686677-90686696 MsG0480023867.01.T01:CDS 55.0%
GCCACAGGATCCATCTTCCC+AGG + Chr4:90686698-90686717 None:intergenic 60.0%
TGGACGACCTCCTGGGAAGA+TGG - Chr4:90686685-90686704 MsG0480023867.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 90686013 90687364 90686013 ID=MsG0480023867.01;Name=MsG0480023867.01
Chr4 mRNA 90686013 90687364 90686013 ID=MsG0480023867.01.T01;Parent=MsG0480023867.01;Name=MsG0480023867.01.T01;_AED=0.28;_eAED=0.28;_QI=0|0.8|0.83|0.83|1|1|6|362|158
Chr4 exon 90686013 90686527 90686013 ID=MsG0480023867.01.T01:exon:33874;Parent=MsG0480023867.01.T01
Chr4 exon 90686620 90686696 90686620 ID=MsG0480023867.01.T01:exon:33873;Parent=MsG0480023867.01.T01
Chr4 exon 90686794 90686852 90686794 ID=MsG0480023867.01.T01:exon:33872;Parent=MsG0480023867.01.T01
Chr4 exon 90686971 90687044 90686971 ID=MsG0480023867.01.T01:exon:33871;Parent=MsG0480023867.01.T01
Chr4 exon 90687148 90687197 90687148 ID=MsG0480023867.01.T01:exon:33870;Parent=MsG0480023867.01.T01
Chr4 exon 90687301 90687364 90687301 ID=MsG0480023867.01.T01:exon:33869;Parent=MsG0480023867.01.T01
Chr4 CDS 90687301 90687364 90687301 ID=MsG0480023867.01.T01:cds;Parent=MsG0480023867.01.T01
Chr4 CDS 90687148 90687197 90687148 ID=MsG0480023867.01.T01:cds;Parent=MsG0480023867.01.T01
Chr4 CDS 90686971 90687044 90686971 ID=MsG0480023867.01.T01:cds;Parent=MsG0480023867.01.T01
Chr4 CDS 90686794 90686852 90686794 ID=MsG0480023867.01.T01:cds;Parent=MsG0480023867.01.T01
Chr4 CDS 90686620 90686696 90686620 ID=MsG0480023867.01.T01:cds;Parent=MsG0480023867.01.T01
Chr4 CDS 90686375 90686527 90686375 ID=MsG0480023867.01.T01:cds;Parent=MsG0480023867.01.T01
Chr4 three_prime_UTR 90686013 90686374 90686013 ID=MsG0480023867.01.T01:three_prime_utr;Parent=MsG0480023867.01.T01
Gene Sequence

>MsG0480023867.01.T01

ATGGTGCAGTATAGTCGTGTCATGTCTTATAGTTTGAAAATTTTGTCATACCCCCTTGTATCCAGTTGGAGCCATAGTTTCATATTACAAGATTCCATTGAAGCAGTACTTGTGATATTTGACGACTTAGATTTGCCTTTTGCTAAATTACGGCTTCTACCAAAGGGTGGACATGGAGGTCACAATGGAATGAAGAGTGTTATTAATCACTTGAAAGGGAATTCTGGTTTTCCTCGCTTAAGAATTGGCATTGGACGACCTCCTGGGAAGATGGATCCTGTGGCATTTGTTCTTCGACCTTTCACTAAACAGGAAAAGGAAGAGCTGAATTTTACCTTACAAGATGGATTAGAAGCCGTGCGGATTATTTTGTTGGACGGATTTGATAAAGTGCAACATTTGTTAATAGTGCCAAAAAGATGGAGCAAACAGCTGAGGATTGCACACATTGAACATCCTTTTGACAATTATATATAA

Protein sequence

>MsG0480023867.01.T01

MVQYSRVMSYSLKILSYPLVSSWSHSFILQDSIEAVLVIFDDLDLPFAKLRLLPKGGHGGHNGMKSVINHLKGNSGFPRLRIGIGRPPGKMDPVAFVLRPFTKQEKEELNFTLQDGLEAVRIILLDGFDKVQHLLIVPKRWSKQLRIAHIEHPFDNYI*