Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022921.01.T01 | XP_013446518.1 | 94.569 | 313 | 13 | 2 | 1 | 310 | 1 | 312 | 0 | 594 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048773.01 | MsG0480022921.01 | 0.823013 | 1.741077e-53 | 1.481321e-50 |
| MsG0080048802.01 | MsG0480022921.01 | 0.802059 | 6.712485e-49 | 3.256552e-46 |
| MsG0080048849.01 | MsG0480022921.01 | 0.819937 | 8.934005e-53 | 6.971657e-50 |
| MsG0080048878.01 | MsG0480022921.01 | 0.833153 | 6.279576e-56 | 7.170620e-53 |
| MsG0080049011.01 | MsG0480022921.01 | 0.835530 | 1.590448e-56 | 1.950149e-53 |
| MsG0080049040.01 | MsG0480022921.01 | 0.825097 | 5.643002e-54 | 5.094086e-51 |
| MsG0080049068.01 | MsG0480022921.01 | 0.840935 | 6.442270e-58 | 9.319001e-55 |
| MsG0180000113.01 | MsG0480022921.01 | 0.821551 | 3.801418e-53 | 3.102490e-50 |
| MsG0180000354.01 | MsG0480022921.01 | 0.824322 | 8.594231e-54 | 7.586623e-51 |
| MsG0180000355.01 | MsG0480022921.01 | 0.849426 | 3.259378e-60 | 6.176005e-57 |
| MsG0180000509.01 | MsG0480022921.01 | 0.807196 | 5.688198e-50 | 3.148379e-47 |
| MsG0180000785.01 | MsG0480022921.01 | 0.806496 | 7.998001e-50 | 4.347503e-47 |
| MsG0180001270.01 | MsG0480022921.01 | 0.809167 | 2.162803e-50 | 1.260743e-47 |
| MsG0180002700.01 | MsG0480022921.01 | 0.816030 | 6.821767e-52 | 4.780285e-49 |
| MsG0180003353.01 | MsG0480022921.01 | 0.840048 | 1.099368e-57 | 1.547403e-54 |
| MsG0180003821.01 | MsG0480022921.01 | 0.886389 | 3.567737e-72 | 2.543596e-68 |
| MsG0180004103.01 | MsG0480022921.01 | 0.803621 | 3.194906e-49 | 1.613072e-46 |
| MsG0180004276.01 | MsG0480022921.01 | 0.833639 | 4.750159e-56 | 5.503153e-53 |
| MsG0180004493.01 | MsG0480022921.01 | 0.819452 | 1.153007e-52 | 8.876291e-50 |
| MsG0180004750.01 | MsG0480022921.01 | 0.829465 | 5.071272e-55 | 5.194360e-52 |
| MsG0180005807.01 | MsG0480022921.01 | 0.815756 | 7.852730e-52 | 5.461847e-49 |
| MsG0180006031.01 | MsG0480022921.01 | 0.802021 | 6.836070e-49 | 3.313204e-46 |
| MsG0280010564.01 | MsG0480022921.01 | 0.813150 | 2.961500e-51 | 1.918762e-48 |
| MsG0280011073.01 | MsG0480022921.01 | 0.821913 | 3.135760e-53 | 2.585597e-50 |
| MsG0280011180.01 | MsG0480022921.01 | 0.801700 | 7.954879e-49 | 3.824086e-46 |
| MsG0280011255.01 | MsG0480022921.01 | 0.809101 | 2.234539e-50 | 1.300250e-47 |
| MsG0480018644.01 | MsG0480022921.01 | 0.802189 | 6.311784e-49 | 3.072192e-46 |
| MsG0480018848.01 | MsG0480022921.01 | 0.802444 | 5.593629e-49 | 2.740511e-46 |
| MsG0480018850.01 | MsG0480022921.01 | 0.877593 | 5.547387e-69 | 2.804499e-65 |
| MsG0480018911.01 | MsG0480022921.01 | 0.831371 | 1.734212e-55 | 1.878173e-52 |
| MsG0480019007.01 | MsG0480022921.01 | 0.813932 | 1.992997e-51 | 1.318994e-48 |
| MsG0480020024.01 | MsG0480022921.01 | 0.817393 | 3.375946e-52 | 2.455577e-49 |
| MsG0480020408.01 | MsG0480022921.01 | 0.802187 | 6.318136e-49 | 3.075154e-46 |
| MsG0480020690.01 | MsG0480022921.01 | 0.849291 | 3.553544e-60 | 6.705035e-57 |
| MsG0480021150.01 | MsG0480022921.01 | -0.824492 | 7.838142e-54 | 6.953399e-51 |
| MsG0480022200.01 | MsG0480022921.01 | 0.804252 | 2.362116e-49 | 1.212043e-46 |
| MsG0480022239.01 | MsG0480022921.01 | 0.831567 | 1.551676e-55 | 1.690432e-52 |
| MsG0480022355.01 | MsG0480022921.01 | 0.810256 | 1.261879e-50 | 7.570044e-48 |
| MsG0480022401.01 | MsG0480022921.01 | 0.834739 | 2.517918e-56 | 3.014764e-53 |
| MsG0480022643.01 | MsG0480022921.01 | 0.813871 | 2.055095e-51 | 1.357913e-48 |
| MsG0480022647.01 | MsG0480022921.01 | 0.822320 | 2.523177e-53 | 2.104874e-50 |
| MsG0480022921.01 | MsG0480023073.01 | 0.836200 | 1.075646e-56 | 1.346156e-53 |
| MsG0480022921.01 | MsG0480023367.01 | 0.816457 | 5.476455e-52 | 3.882301e-49 |
| MsG0480022921.01 | MsG0480023384.01 | 0.827482 | 1.526796e-54 | 1.475900e-51 |
| MsG0480022921.01 | MsG0480023652.01 | 0.847491 | 1.117991e-59 | 1.990601e-56 |
| MsG0480022921.01 | MsG0480023867.01 | 0.825814 | 3.818617e-54 | 3.518231e-51 |
| MsG0480022921.01 | MsG0580024930.01 | -0.809996 | 1.435631e-50 | 8.554567e-48 |
| MsG0480022921.01 | MsG0580025502.01 | 0.810213 | 1.289089e-50 | 7.724149e-48 |
| MsG0480022921.01 | MsG0580025971.01 | 0.820300 | 7.379185e-53 | 5.816364e-50 |
| MsG0480022921.01 | MsG0580026206.01 | 0.817130 | 3.868842e-52 | 2.793756e-49 |
| MsG0480022921.01 | MsG0580026921.01 | 0.801258 | 9.802899e-49 | 4.660287e-46 |
| MsG0480022921.01 | MsG0580027394.01 | 0.813672 | 2.273750e-51 | 1.494313e-48 |
| MsG0480022921.01 | MsG0580028059.01 | 0.832531 | 8.962896e-56 | 1.004569e-52 |
| MsG0480022921.01 | MsG0580029529.01 | 0.818955 | 1.495688e-52 | 1.135789e-49 |
| MsG0480022921.01 | MsG0580029734.01 | 0.824873 | 6.376298e-54 | 5.719382e-51 |
| MsG0480022921.01 | MsG0580029905.01 | 0.825193 | 5.357652e-54 | 4.849679e-51 |
| MsG0480022921.01 | MsG0580029912.01 | 0.804738 | 1.870410e-49 | 9.719616e-47 |
| MsG0480022921.01 | MsG0580029914.01 | 0.819063 | 1.413548e-52 | 1.076528e-49 |
| MsG0480022921.01 | MsG0680031000.01 | 0.822424 | 2.386129e-53 | 1.996542e-50 |
| MsG0480022921.01 | MsG0680031010.01 | 0.844779 | 6.119968e-59 | 9.987356e-56 |
| MsG0480022921.01 | MsG0680031469.01 | 0.877806 | 4.676246e-69 | 2.383697e-65 |
| MsG0480022921.01 | MsG0680031939.01 | 0.809748 | 1.623442e-50 | 9.610947e-48 |
| MsG0480022921.01 | MsG0680032830.01 | 0.826555 | 2.544586e-54 | 2.394194e-51 |
| MsG0480022921.01 | MsG0680033377.01 | 0.825076 | 5.708950e-54 | 5.150567e-51 |
| MsG0480022921.01 | MsG0680034064.01 | 0.849011 | 4.251427e-60 | 7.949864e-57 |
| MsG0480022921.01 | MsG0680035731.01 | 0.803835 | 2.884374e-49 | 1.464335e-46 |
| MsG0480022921.01 | MsG0780035960.01 | 0.801082 | 1.064846e-48 | 5.040085e-46 |
| MsG0480022921.01 | MsG0780035992.01 | 0.807029 | 6.170904e-50 | 3.400790e-47 |
| MsG0480022921.01 | MsG0780036385.01 | 0.806304 | 8.778216e-50 | 4.748330e-47 |
| MsG0480022921.01 | MsG0780036659.01 | 0.804350 | 2.253516e-49 | 1.159292e-46 |
| MsG0480022921.01 | MsG0780037399.01 | 0.821881 | 3.189631e-53 | 2.627744e-50 |
| MsG0480022921.01 | MsG0780038784.01 | 0.806446 | 8.194405e-50 | 4.448640e-47 |
| MsG0480022921.01 | MsG0780038846.01 | 0.805052 | 1.607791e-49 | 8.422281e-47 |
| MsG0480022921.01 | MsG0780040277.01 | 0.829579 | 4.756212e-55 | 4.887929e-52 |
| MsG0480022921.01 | MsG0780040284.01 | 0.846480 | 2.115737e-59 | 3.646925e-56 |
| MsG0480022921.01 | MsG0780040443.01 | 0.804149 | 2.480792e-49 | 1.269662e-46 |
| MsG0480022921.01 | MsG0780040825.01 | -0.820464 | 6.763982e-53 | 5.356107e-50 |
| MsG0480022921.01 | MsG0780041729.01 | -0.855388 | 6.524333e-62 | 1.503945e-58 |
| MsG0480022921.01 | MsG0780041747.01 | 0.833090 | 6.509692e-56 | 7.419742e-53 |
| MsG0480022921.01 | MsG0880042890.01 | 0.806982 | 6.312958e-50 | 3.474798e-47 |
| MsG0480022921.01 | MsG0880043024.01 | 0.829735 | 4.358235e-55 | 4.499923e-52 |
| MsG0480022921.01 | MsG0880044005.01 | 0.839328 | 1.692668e-57 | 2.329666e-54 |
| MsG0480022921.01 | MsG0880044392.01 | 0.827566 | 1.457543e-54 | 1.412277e-51 |
| MsG0480022921.01 | MsG0880045044.01 | 0.807991 | 3.857017e-50 | 2.179527e-47 |
| MsG0480022921.01 | MsG0880045684.01 | 0.808774 | 2.625199e-50 | 1.514183e-47 |
| MsG0480022921.01 | MsG0880046382.01 | 0.822514 | 2.274958e-53 | 1.908334e-50 |
| MsG0480022921.01 | MsG0880046765.01 | 0.814711 | 1.340378e-51 | 9.060256e-49 |
| MsG0480022921.01 | MsG0880046867.01 | 0.833210 | 6.077301e-56 | 6.951600e-53 |
| MsG0480022921.01 | MsG0880047152.01 | 0.807034 | 6.157032e-50 | 3.393506e-47 |
| MsG0480022921.01 | MsG0880047422.01 | -0.811871 | 5.635865e-51 | 3.528755e-48 |
| MsG0480022921.01 | MsG0880047442.01 | 0.828932 | 6.826956e-55 | 6.883573e-52 |
| MsG0480022921.01 | MsG0880047656.01 | 0.854043 | 1.600499e-61 | 3.528893e-58 |
| MsG0480022921.01 | MsG0880047696.01 | 0.841464 | 4.676755e-58 | 6.877918e-55 |
| MsG0480022921.01 | MsG0880047703.01 | 0.869957 | 2.097828e-66 | 8.019260e-63 |
| MsG0380015603.01 | MsG0480022921.01 | 0.818092 | 2.347695e-52 | 1.740545e-49 |
| MsG0380015703.01 | MsG0480022921.01 | 0.821418 | 4.081085e-53 | 3.318355e-50 |
| MsG0380016194.01 | MsG0480022921.01 | 0.841188 | 5.527414e-58 | 8.058296e-55 |
| MsG0380016284.01 | MsG0480022921.01 | 0.814136 | 1.796790e-51 | 1.195805e-48 |
| MsG0380017087.01 | MsG0480022921.01 | 0.819249 | 1.282203e-52 | 9.815307e-50 |
| MsG0380017338.01 | MsG0480022921.01 | 0.805267 | 1.449716e-49 | 7.635911e-47 |
| MsG0380017703.01 | MsG0480022921.01 | 0.803307 | 3.711063e-49 | 1.858559e-46 |
| MsG0280007502.01 | MsG0480022921.01 | 0.811313 | 7.451483e-51 | 4.597116e-48 |
| MsG0280007903.01 | MsG0480022921.01 | 0.804075 | 2.570691e-49 | 1.313205e-46 |
| MsG0280007976.01 | MsG0480022921.01 | 0.805040 | 1.616985e-49 | 8.467700e-47 |
| MsG0280008098.01 | MsG0480022921.01 | 0.800541 | 1.373159e-48 | 6.410525e-46 |
| MsG0280008394.01 | MsG0480022921.01 | 0.811836 | 5.736473e-51 | 3.588347e-48 |
| MsG0280008928.01 | MsG0480022921.01 | 0.822116 | 2.814237e-53 | 2.333857e-50 |
| MsG0280008929.01 | MsG0480022921.01 | 0.843439 | 1.399962e-58 | 2.190505e-55 |
| MsG0280009685.01 | MsG0480022921.01 | 0.867011 | 1.873295e-65 | 6.441505e-62 |
| MsG0280010288.01 | MsG0480022921.01 | 0.803583 | 3.253381e-49 | 1.641100e-46 |
| MsG0280010503.01 | MsG0480022921.01 | 0.829744 | 4.336798e-55 | 4.479081e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022921.01.T01 | MTR_8g080020 | 94.569 | 313 | 13 | 2 | 1 | 310 | 1 | 312 | 0.0 | 594 |
| MsG0480022921.01.T01 | MTR_2g088950 | 40.574 | 244 | 125 | 6 | 75 | 304 | 103 | 340 | 6.11e-54 | 179 |
| MsG0480022921.01.T01 | MTR_2g437670 | 40.574 | 244 | 125 | 6 | 75 | 304 | 103 | 340 | 8.60e-54 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480022921.01.T01 | AT1G67660 | 65.934 | 273 | 81 | 3 | 49 | 309 | 33 | 305 | 5.16e-134 | 382 |
| MsG0480022921.01.T01 | AT1G67660 | 65.934 | 273 | 81 | 3 | 49 | 309 | 62 | 334 | 1.07e-133 | 383 |
| MsG0480022921.01.T01 | AT1G67660 | 65.934 | 273 | 81 | 3 | 49 | 309 | 83 | 355 | 1.38e-133 | 383 |
| MsG0480022921.01.T01 | AT1G13810 | 36.032 | 247 | 138 | 5 | 75 | 306 | 60 | 301 | 2.61e-47 | 161 |
Find 69 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATTACATGCCTCAAGTTC+AGG | 0.257165 | 4:+78607934 | MsG0480022921.01.T01:CDS |
| TCAGGGTCAAATGGAGATTA+TGG | 0.260537 | 4:+78607952 | MsG0480022921.01.T01:CDS |
| AGTTAAGTGTCCTTATAATA+AGG | 0.269945 | 4:+78607871 | MsG0480022921.01.T01:CDS |
| TTGTTAGAAATCGTGAATAT+TGG | 0.278665 | 4:+78608032 | MsG0480022921.01.T01:CDS |
| TTCAAAGAAATGCTATGAAT+TGG | 0.293866 | 4:+78607699 | MsG0480022921.01.T01:CDS |
| TTAGTACTGCATTAGGGTTT+TGG | 0.302003 | 4:+78607606 | MsG0480022921.01.T01:CDS |
| TGTTCTTGGTACCGGAATAT+TGG | 0.306883 | 4:+78607847 | MsG0480022921.01.T01:CDS |
| TCAGGCTTACCCTTATTATA+AGG | 0.309587 | 4:-78607881 | None:intergenic |
| GCAAGGGAAGCTTTGTTGTT+GGG | 0.320750 | 4:+78608106 | MsG0480022921.01.T01:CDS |
| AACAACAAAGCTTCCCTTGC+CGG | 0.337209 | 4:-78608103 | None:intergenic |
| ATGGGATTCTCAGAGAGTTT+TGG | 0.342835 | 4:+78608065 | MsG0480022921.01.T01:CDS |
| TCAAAGAAATGCTATGAATT+GGG | 0.356628 | 4:+78607700 | MsG0480022921.01.T01:CDS |
| AGCACATTTAGTACTGCATT+AGG | 0.371913 | 4:+78607599 | MsG0480022921.01.T01:CDS |
| GCGTCGCCTGACGGTGTTCT+TGG | 0.374201 | 4:+78607833 | MsG0480022921.01.T01:CDS |
| TGTTAGAAATCGTGAATATT+GGG | 0.374315 | 4:+78608033 | MsG0480022921.01.T01:CDS |
| TTCCGCAACGTTCGGAAGAA+TGG | 0.380577 | 4:+78607546 | MsG0480022921.01.T01:CDS |
| ACACTTAACTTCCAATATTC+CGG | 0.387205 | 4:-78607858 | None:intergenic |
| AATGGGTTGATTTGTATTGT+TGG | 0.389762 | 4:+78607981 | MsG0480022921.01.T01:CDS |
| TTCCATGGCAAACCTGTCTC+AGG | 0.395522 | 4:-78607899 | None:intergenic |
| ATTCGAAACAGTCTTATGAT+TGG | 0.396472 | 4:+78607804 | MsG0480022921.01.T01:CDS |
| GCACATTTAGTACTGCATTA+GGG | 0.406562 | 4:+78607600 | MsG0480022921.01.T01:CDS |
| TTGGTGGGAAAACGTGATTC+CGG | 0.412330 | 4:+78608084 | MsG0480022921.01.T01:CDS |
| CCTAGCAGAATTAAGCCATA+TGG | 0.413449 | 4:+78607413 | MsG0480022921.01.T01:CDS |
| GCCTCAAGTTCAGGGTCAAA+TGG | 0.417390 | 4:+78607943 | MsG0480022921.01.T01:CDS |
| GGCAAGGGAAGCTTTGTTGT+TGG | 0.439360 | 4:+78608105 | MsG0480022921.01.T01:CDS |
| GTTGTTGGGGTCTAAAGATC+AGG | 0.442016 | 4:+78608120 | MsG0480022921.01.T01:CDS |
| TTAATAATCAAAGAAGTTGT+TGG | 0.449938 | 4:-78607479 | None:intergenic |
| CGAAATATAGTACTTCCATT+TGG | 0.450018 | 4:-78608007 | None:intergenic |
| GGCTGATGTTCCGCAACGTT+CGG | 0.452606 | 4:+78607538 | MsG0480022921.01.T01:CDS |
| TGGTGAGGTAGTAGTACATA+TGG | 0.459125 | 4:-78607459 | None:intergenic |
| GTTAAGTGTCCTTATAATAA+GGG | 0.460463 | 4:+78607872 | MsG0480022921.01.T01:CDS |
| CAAGGGAAGCTTTGTTGTTG+GGG | 0.465604 | 4:+78608107 | MsG0480022921.01.T01:CDS |
| TGGGAGGGAGGTGACTTCGA+TGG | 0.467844 | 4:+78607769 | MsG0480022921.01.T01:CDS |
| GGGAGGGAGGTGACTTCGAT+GGG | 0.473979 | 4:+78607770 | MsG0480022921.01.T01:CDS |
| TATTACATGCCTCAAGTTCA+GGG | 0.475534 | 4:+78607935 | MsG0480022921.01.T01:CDS |
| AGCATTTGCTTCACCGGCAC+CGG | 0.476435 | 4:+78607517 | MsG0480022921.01.T01:CDS |
| ATTTACAGCATTTGCTTCAC+CGG | 0.476975 | 4:+78607511 | MsG0480022921.01.T01:CDS |
| GCCTCTACACATAAATTGAC+TGG | 0.480024 | 4:+78608160 | MsG0480022921.01.T01:CDS |
| AATGGATATGAGAATTTCAT+CGG | 0.483975 | 4:+78607337 | None:intergenic |
| GAACGTTGCGGAACATCAGC+CGG | 0.496988 | 4:-78607536 | None:intergenic |
| TGCGGAACATCAGCCGGTGC+CGG | 0.504036 | 4:-78607530 | None:intergenic |
| AAACAGTCTTATGATTGGCT+TGG | 0.505920 | 4:+78607809 | MsG0480022921.01.T01:CDS |
| GTCAATTTATGTGTAGAGGC+AGG | 0.506154 | 4:-78608157 | None:intergenic |
| AATAAGGGTAAGCCTGAGAC+AGG | 0.506865 | 4:+78607887 | MsG0480022921.01.T01:CDS |
| TTGTATTGTTGGACGCCAAA+TGG | 0.508428 | 4:+78607992 | MsG0480022921.01.T01:CDS |
| TCCATTTGACCCTGAACTTG+AGG | 0.510243 | 4:-78607944 | None:intergenic |
| CTATTATGCAGAGAAATTGC+AGG | 0.517198 | 4:+78608226 | MsG0480022921.01.T01:CDS |
| GAATATTGGGATATAATACA+TGG | 0.523100 | 4:+78608046 | MsG0480022921.01.T01:CDS |
| CCATATGGCTTAATTCTGCT+AGG | 0.530559 | 4:-78607413 | None:intergenic |
| TGGCTTGGCGCGTCGCCTGA+CGG | 0.530902 | 4:+78607824 | MsG0480022921.01.T01:CDS |
| AGAATGGTTCGCTCTTCGTA+AGG | 0.534956 | 4:+78607562 | MsG0480022921.01.T01:CDS |
| CCGGTACCAAGAACACCGTC+AGG | 0.549096 | 4:-78607839 | None:intergenic |
| TCCAGTCAATTTATGTGTAG+AGG | 0.552337 | 4:-78608161 | None:intergenic |
| CCTGACGGTGTTCTTGGTAC+CGG | 0.552937 | 4:+78607839 | MsG0480022921.01.T01:CDS |
| CAAAGAAATGCTATGAATTG+GGG | 0.562081 | 4:+78607701 | MsG0480022921.01.T01:CDS |
| GGAAAACGTGATTCCGGCAA+GGG | 0.564224 | 4:+78608090 | MsG0480022921.01.T01:CDS |
| ACTTGAGGCATGTAATAGAA+TGG | 0.565196 | 4:-78607929 | None:intergenic |
| GAATCTAATCAAGCTAGAGT+TGG | 0.567061 | 4:-78607366 | None:intergenic |
| GAAGCAAATGCTGTAAATGA+TGG | 0.578930 | 4:-78607506 | None:intergenic |
| AGCCTGAGACAGGTTTGCCA+TGG | 0.592845 | 4:+78607897 | MsG0480022921.01.T01:CDS |
| TCTAGCTTGATTAGATTCAG+TGG | 0.596966 | 4:+78607371 | MsG0480022921.01.T01:CDS |
| AATCAAAGAAGTTGTTGGTG+AGG | 0.598502 | 4:-78607474 | None:intergenic |
| AACCATTCTTCCGAACGTTG+CGG | 0.616608 | 4:-78607548 | None:intergenic |
| GGGAAAACGTGATTCCGGCA+AGG | 0.625422 | 4:+78608089 | MsG0480022921.01.T01:CDS |
| GAATTGGGGAGTGCTTAATG+AGG | 0.644725 | 4:+78607715 | MsG0480022921.01.T01:CDS |
| TTGGGGAGTGCTTAATGAGG+CGG | 0.645877 | 4:+78607718 | MsG0480022921.01.T01:CDS |
| GTGGTCGTCGTGCTGAGTTG+TGG | 0.647045 | 4:+78607633 | MsG0480022921.01.T01:CDS |
| AATATTGGGATATAATACAT+GGG | 0.663115 | 4:+78608047 | MsG0480022921.01.T01:CDS |
| GCAAATGCTGTAAATGATGG+AGG | 0.672307 | 4:-78607503 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAGTATACAAAAATTACT+GGG | + | Chr4:78607750-78607769 | MsG0480022921.01.T01:CDS | 15.0% |
| !! | AATATTGGGATATAATACAT+GGG | + | Chr4:78608047-78608066 | MsG0480022921.01.T01:CDS | 20.0% |
| !! | TTAATAATCAAAGAAGTTGT+TGG | - | Chr4:78607482-78607501 | None:intergenic | 20.0% |
| !!! | GAAAAGTATACAAAAATTAC+TGG | + | Chr4:78607749-78607768 | MsG0480022921.01.T01:CDS | 20.0% |
| ! | AGTTAAGTGTCCTTATAATA+AGG | + | Chr4:78607871-78607890 | MsG0480022921.01.T01:CDS | 25.0% |
| ! | GAATATTGGGATATAATACA+TGG | + | Chr4:78608046-78608065 | MsG0480022921.01.T01:CDS | 25.0% |
| ! | GTTAAGTGTCCTTATAATAA+GGG | + | Chr4:78607872-78607891 | MsG0480022921.01.T01:CDS | 25.0% |
| ! | TCAAAGAAATGCTATGAATT+GGG | + | Chr4:78607700-78607719 | MsG0480022921.01.T01:CDS | 25.0% |
| ! | TGTTAGAAATCGTGAATATT+GGG | + | Chr4:78608033-78608052 | MsG0480022921.01.T01:CDS | 25.0% |
| ! | TTCAAAGAAATGCTATGAAT+TGG | + | Chr4:78607699-78607718 | MsG0480022921.01.T01:CDS | 25.0% |
| ! | TTGTTAGAAATCGTGAATAT+TGG | + | Chr4:78608032-78608051 | MsG0480022921.01.T01:CDS | 25.0% |
| !! | CTAAAATGATCTTTTCCATA+TGG | - | Chr4:78607431-78607450 | None:intergenic | 25.0% |
| ACACTTAACTTCCAATATTC+CGG | - | Chr4:78607861-78607880 | None:intergenic | 30.0% | |
| AGTATACAAAAATTACTGGG+AGG | + | Chr4:78607753-78607772 | MsG0480022921.01.T01:CDS | 30.0% | |
| CAAAGAAATGCTATGAATTG+GGG | + | Chr4:78607701-78607720 | MsG0480022921.01.T01:CDS | 30.0% | |
| CGAAATATAGTACTTCCATT+TGG | - | Chr4:78608010-78608029 | None:intergenic | 30.0% | |
| GTATACAAAAATTACTGGGA+GGG | + | Chr4:78607754-78607773 | MsG0480022921.01.T01:CDS | 30.0% | |
| ! | ATTCGAAACAGTCTTATGAT+TGG | + | Chr4:78607804-78607823 | MsG0480022921.01.T01:CDS | 30.0% |
| ! | GGAGATTATGGATTTTGAAT+GGG | + | Chr4:78607964-78607983 | MsG0480022921.01.T01:CDS | 30.0% |
| ! | TGGAGATTATGGATTTTGAA+TGG | + | Chr4:78607963-78607982 | MsG0480022921.01.T01:CDS | 30.0% |
| !! | AATGGGTTGATTTGTATTGT+TGG | + | Chr4:78607981-78608000 | MsG0480022921.01.T01:CDS | 30.0% |
| AATCAAAGAAGTTGTTGGTG+AGG | - | Chr4:78607477-78607496 | None:intergenic | 35.0% | |
| ACTTGAGGCATGTAATAGAA+TGG | - | Chr4:78607932-78607951 | None:intergenic | 35.0% | |
| AGCACATTTAGTACTGCATT+AGG | + | Chr4:78607599-78607618 | MsG0480022921.01.T01:CDS | 35.0% | |
| ATTTACAGCATTTGCTTCAC+CGG | + | Chr4:78607511-78607530 | MsG0480022921.01.T01:CDS | 35.0% | |
| CTATTATGCAGAGAAATTGC+AGG | + | Chr4:78608226-78608245 | MsG0480022921.01.T01:CDS | 35.0% | |
| GAAGCAAATGCTGTAAATGA+TGG | - | Chr4:78607509-78607528 | None:intergenic | 35.0% | |
| GAATCTAATCAAGCTAGAGT+TGG | - | Chr4:78607369-78607388 | None:intergenic | 35.0% | |
| GCACATTTAGTACTGCATTA+GGG | + | Chr4:78607600-78607619 | MsG0480022921.01.T01:CDS | 35.0% | |
| TATTACATGCCTCAAGTTCA+GGG | + | Chr4:78607935-78607954 | MsG0480022921.01.T01:CDS | 35.0% | |
| TCAGGCTTACCCTTATTATA+AGG | - | Chr4:78607884-78607903 | None:intergenic | 35.0% | |
| TCCAGTCAATTTATGTGTAG+AGG | - | Chr4:78608164-78608183 | None:intergenic | 35.0% | |
| ! | AAACAGTCTTATGATTGGCT+TGG | + | Chr4:78607809-78607828 | MsG0480022921.01.T01:CDS | 35.0% |
| ! | TAGAATGGCATAGTTTTCCA+TGG | - | Chr4:78607917-78607936 | None:intergenic | 35.0% |
| ! | TCTAGCTTGATTAGATTCAG+TGG | + | Chr4:78607371-78607390 | MsG0480022921.01.T01:CDS | 35.0% |
| ! | TTAGTACTGCATTAGGGTTT+TGG | + | Chr4:78607606-78607625 | MsG0480022921.01.T01:CDS | 35.0% |
| CCATATGGCTTAATTCTGCT+AGG | - | Chr4:78607416-78607435 | None:intergenic | 40.0% | |
| CCTAGCAGAATTAAGCCATA+TGG | + | Chr4:78607413-78607432 | MsG0480022921.01.T01:CDS | 40.0% | |
| CTATTACATGCCTCAAGTTC+AGG | + | Chr4:78607934-78607953 | MsG0480022921.01.T01:CDS | 40.0% | |
| GCAAATGCTGTAAATGATGG+AGG | - | Chr4:78607506-78607525 | None:intergenic | 40.0% | |
| GCCTCTACACATAAATTGAC+TGG | + | Chr4:78608160-78608179 | MsG0480022921.01.T01:CDS | 40.0% | |
| GTCAATTTATGTGTAGAGGC+AGG | - | Chr4:78608160-78608179 | None:intergenic | 40.0% | |
| TACAAAAATTACTGGGAGGG+AGG | + | Chr4:78607757-78607776 | MsG0480022921.01.T01:CDS | 40.0% | |
| TCAGGGTCAAATGGAGATTA+TGG | + | Chr4:78607952-78607971 | MsG0480022921.01.T01:CDS | 40.0% | |
| TGGTGAGGTAGTAGTACATA+TGG | - | Chr4:78607462-78607481 | None:intergenic | 40.0% | |
| TTGTATTGTTGGACGCCAAA+TGG | + | Chr4:78607992-78608011 | MsG0480022921.01.T01:CDS | 40.0% | |
| ! | ATGGGATTCTCAGAGAGTTT+TGG | + | Chr4:78608065-78608084 | MsG0480022921.01.T01:CDS | 40.0% |
| ! | TGTTCTTGGTACCGGAATAT+TGG | + | Chr4:78607847-78607866 | MsG0480022921.01.T01:CDS | 40.0% |
| !! | GATTCTCAGAGAGTTTTGGT+GGG | + | Chr4:78608069-78608088 | MsG0480022921.01.T01:CDS | 40.0% |
| !!! | ACTGCATTAGGGTTTTGGAA+AGG | + | Chr4:78607611-78607630 | MsG0480022921.01.T01:CDS | 40.0% |
| AACAACAAAGCTTCCCTTGC+CGG | - | Chr4:78608106-78608125 | None:intergenic | 45.0% | |
| AACCATTCTTCCGAACGTTG+CGG | - | Chr4:78607551-78607570 | None:intergenic | 45.0% | |
| AATAAGGGTAAGCCTGAGAC+AGG | + | Chr4:78607887-78607906 | MsG0480022921.01.T01:CDS | 45.0% | |
| AGAATGGTTCGCTCTTCGTA+AGG | + | Chr4:78607562-78607581 | MsG0480022921.01.T01:CDS | 45.0% | |
| GTTGTTGGGGTCTAAAGATC+AGG | + | Chr4:78608120-78608139 | MsG0480022921.01.T01:CDS | 45.0% | |
| TCCATTTGACCCTGAACTTG+AGG | - | Chr4:78607947-78607966 | None:intergenic | 45.0% | |
| TTGGTGGGAAAACGTGATTC+CGG | + | Chr4:78608084-78608103 | MsG0480022921.01.T01:CDS | 45.0% | |
| !! | CAAGGGAAGCTTTGTTGTTG+GGG | + | Chr4:78608107-78608126 | MsG0480022921.01.T01:CDS | 45.0% |
| !! | GAATTGGGGAGTGCTTAATG+AGG | + | Chr4:78607715-78607734 | MsG0480022921.01.T01:CDS | 45.0% |
| !! | GCAAGGGAAGCTTTGTTGTT+GGG | + | Chr4:78608106-78608125 | MsG0480022921.01.T01:CDS | 45.0% |
| !! | GGATTCTCAGAGAGTTTTGG+TGG | + | Chr4:78608068-78608087 | MsG0480022921.01.T01:CDS | 45.0% |
| !!! | GCATTAGGGTTTTGGAAAGG+TGG | + | Chr4:78607614-78607633 | MsG0480022921.01.T01:CDS | 45.0% |
| GCCTCAAGTTCAGGGTCAAA+TGG | + | Chr4:78607943-78607962 | MsG0480022921.01.T01:CDS | 50.0% | |
| GGAAAACGTGATTCCGGCAA+GGG | + | Chr4:78608090-78608109 | MsG0480022921.01.T01:CDS | 50.0% | |
| TTCCATGGCAAACCTGTCTC+AGG | - | Chr4:78607902-78607921 | None:intergenic | 50.0% | |
| TTCCGCAACGTTCGGAAGAA+TGG | + | Chr4:78607546-78607565 | MsG0480022921.01.T01:CDS | 50.0% | |
| !! | GGCAAGGGAAGCTTTGTTGT+TGG | + | Chr4:78608105-78608124 | MsG0480022921.01.T01:CDS | 50.0% |
| !! | TTGGGGAGTGCTTAATGAGG+CGG | + | Chr4:78607718-78607737 | MsG0480022921.01.T01:CDS | 50.0% |
| AGCATTTGCTTCACCGGCAC+CGG | + | Chr4:78607517-78607536 | MsG0480022921.01.T01:CDS | 55.0% | |
| AGCCTGAGACAGGTTTGCCA+TGG | + | Chr4:78607897-78607916 | MsG0480022921.01.T01:CDS | 55.0% | |
| CCTGACGGTGTTCTTGGTAC+CGG | + | Chr4:78607839-78607858 | MsG0480022921.01.T01:CDS | 55.0% | |
| GAACGTTGCGGAACATCAGC+CGG | - | Chr4:78607539-78607558 | None:intergenic | 55.0% | |
| GGCTGATGTTCCGCAACGTT+CGG | + | Chr4:78607538-78607557 | MsG0480022921.01.T01:CDS | 55.0% | |
| GGGAAAACGTGATTCCGGCA+AGG | + | Chr4:78608089-78608108 | MsG0480022921.01.T01:CDS | 55.0% | |
| CCGGTACCAAGAACACCGTC+AGG | - | Chr4:78607842-78607861 | None:intergenic | 60.0% | |
| GGGAGGGAGGTGACTTCGAT+GGG | + | Chr4:78607770-78607789 | MsG0480022921.01.T01:CDS | 60.0% | |
| GTGGTCGTCGTGCTGAGTTG+TGG | + | Chr4:78607633-78607652 | MsG0480022921.01.T01:CDS | 60.0% | |
| TGGGAGGGAGGTGACTTCGA+TGG | + | Chr4:78607769-78607788 | MsG0480022921.01.T01:CDS | 60.0% | |
| GCGTCGCCTGACGGTGTTCT+TGG | + | Chr4:78607833-78607852 | MsG0480022921.01.T01:CDS | 65.0% | |
| TGCGGAACATCAGCCGGTGC+CGG | - | Chr4:78607533-78607552 | None:intergenic | 65.0% | |
| ! | TGGCTTGGCGCGTCGCCTGA+CGG | + | Chr4:78607824-78607843 | MsG0480022921.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 78607338 | 78608270 | 78607338 | ID=MsG0480022921.01;Name=MsG0480022921.01 |
| Chr4 | mRNA | 78607338 | 78608270 | 78607338 | ID=MsG0480022921.01.T01;Parent=MsG0480022921.01;Name=MsG0480022921.01.T01;_AED=0.21;_eAED=0.21;_QI=0|-1|0|1|-1|1|1|0|310 |
| Chr4 | exon | 78607338 | 78608270 | 78607338 | ID=MsG0480022921.01.T01:exon:32268;Parent=MsG0480022921.01.T01 |
| Chr4 | CDS | 78607338 | 78608270 | 78607338 | ID=MsG0480022921.01.T01:cds;Parent=MsG0480022921.01.T01 |
| Gene Sequence |
| Protein sequence |
