AlfalfaGEDB | MsG0380014000.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014000.01

KEGG: K02704
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014000.01.T01	KAF6165022.1	83.673	49	8	0	1	49	240	288	5.87E-18	85.5

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014000.01.T01	A0A7J7NDG8	83.673	49	8	0	1	49	240	288	2.80e-18	85.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048611.01	MsG0380014000.01	0.820317	7.311237e-53	5.765593e-50
MsG0080048845.01	MsG0380014000.01	0.820679	6.039453e-53	4.810416e-50
MsG0080049078.01	MsG0380014000.01	0.879086	1.660303e-69	8.882872e-66
MsG0080049079.01	MsG0380014000.01	0.851135	1.081146e-60	2.166880e-57
MsG0080049080.01	MsG0380014000.01	0.816919	4.315142e-52	3.098105e-49
MsG0080049130.01	MsG0380014000.01	0.803014	4.266907e-49	2.120876e-46
MsG0180000843.01	MsG0380014000.01	0.815452	9.178447e-52	6.330708e-49
MsG0180003933.01	MsG0380014000.01	0.815517	8.878059e-52	6.134517e-49
MsG0180004735.01	MsG0380014000.01	0.816073	6.674105e-52	4.682322e-49
MsG0180004987.01	MsG0380014000.01	0.842376	2.685309e-58	4.063870e-55
MsG0180005119.01	MsG0380014000.01	0.822506	2.284688e-53	1.916014e-50
MsG0180005150.01	MsG0380014000.01	0.802150	6.430129e-49	3.126778e-46
MsG0180005568.01	MsG0380014000.01	0.829465	5.070365e-55	5.193455e-52
MsG0180005879.01	MsG0380014000.01	0.814239	1.705155e-51	1.137998e-48
MsG0280006319.01	MsG0380014000.01	0.815837	7.535503e-52	5.252139e-49
MsG0280010926.01	MsG0380014000.01	0.824331	8.553375e-54	7.552587e-51
MsG0280010975.01	MsG0380014000.01	0.833123	6.387682e-56	7.288178e-53
MsG0280011270.01	MsG0380014000.01	0.803155	3.989802e-49	1.990468e-46
MsG0280011411.01	MsG0380014000.01	0.821357	4.214378e-53	3.420873e-50
MsG0380012443.01	MsG0380014000.01	0.815129	1.083071e-51	7.403833e-49
MsG0380013472.01	MsG0380014000.01	0.833872	4.154470e-56	4.847508e-53
MsG0380013530.01	MsG0380014000.01	0.806263	8.955852e-50	4.839558e-47
MsG0380013927.01	MsG0380014000.01	0.834379	3.101674e-56	3.674447e-53
MsG0380013955.01	MsG0380014000.01	0.888435	5.918130e-73	4.584234e-69
MsG0380013958.01	MsG0380014000.01	0.850239	1.930531e-60	3.757385e-57
MsG0380013970.01	MsG0380014000.01	0.878949	1.855547e-69	9.875538e-66
MsG0380013992.01	MsG0380014000.01	0.816761	4.680258e-52	3.345845e-49
MsG0380013996.01	MsG0380014000.01	0.846663	1.885862e-59	3.269938e-56
MsG0380014000.01	MsG0380014007.01	0.872129	4.031817e-67	1.667685e-63
MsG0380014000.01	MsG0380014012.01	0.820693	5.993733e-53	4.775903e-50
MsG0380014000.01	MsG0380014013.01	0.808077	3.697011e-50	2.093864e-47
MsG0380014000.01	MsG0380014022.01	0.876704	1.129929e-68	5.531961e-65
MsG0380014000.01	MsG0380014037.01	0.858890	6.039446e-63	1.565127e-59
MsG0380014000.01	MsG0380014053.01	0.834168	3.502639e-56	4.122617e-53
MsG0380014000.01	MsG0380014729.01	0.801853	7.399799e-49	3.571320e-46
MsG0380014000.01	MsG0380015496.01	0.838652	2.532355e-57	3.414211e-54
MsG0380014000.01	MsG0380015545.01	0.814804	1.278370e-51	8.662902e-49
MsG0380014000.01	MsG0380015547.01	0.808262	3.376692e-50	1.921912e-47
MsG0380014000.01	MsG0380015897.01	0.801502	8.736269e-49	4.178939e-46
MsG0380014000.01	MsG0380015898.01	0.808354	3.228102e-50	1.841772e-47
MsG0380014000.01	MsG0380016194.01	0.815129	1.082577e-51	7.400653e-49
MsG0380014000.01	MsG0380016284.01	0.810350	1.204128e-50	7.241382e-48
MsG0380014000.01	MsG0380016452.01	0.811446	6.971705e-51	4.316623e-48
MsG0380014000.01	MsG0380016459.01	0.820448	6.823025e-53	5.400307e-50
MsG0380014000.01	MsG0380016542.01	0.805558	1.259980e-49	6.686244e-47
MsG0380014000.01	MsG0380016751.01	0.845870	3.100327e-59	5.239863e-56
MsG0380014000.01	MsG0380016762.01	0.856769	2.571458e-62	6.203901e-59
MsG0380014000.01	MsG0380017739.01	0.816646	4.967064e-52	3.539674e-49
MsG0380014000.01	MsG0480018089.01	0.847895	8.658439e-60	1.561516e-56
MsG0380014000.01	MsG0480018323.01	0.801092	1.059721e-48	5.017256e-46
MsG0380014000.01	MsG0480018324.01	0.803068	4.157622e-49	2.069431e-46
MsG0380014000.01	MsG0480018983.01	0.801305	9.588298e-49	4.563675e-46
MsG0380014000.01	MsG0480019592.01	0.825696	4.072017e-54	3.739019e-51
MsG0380014000.01	MsG0480019641.01	0.802560	5.294263e-49	2.601404e-46
MsG0380014000.01	MsG0480019971.01	0.815933	7.170462e-52	5.011151e-49
MsG0380014000.01	MsG0480020049.01	0.810533	1.099273e-50	6.642874e-48
MsG0380014000.01	MsG0480020350.01	0.843484	1.361978e-58	2.133998e-55
MsG0380014000.01	MsG0480020408.01	0.815978	7.007025e-52	4.902949e-49
MsG0380014000.01	MsG0480020533.01	0.812872	3.406195e-51	2.190527e-48
MsG0380014000.01	MsG0480021168.01	0.806923	6.499284e-50	3.571800e-47
MsG0380014000.01	MsG0480021294.01	0.825972	3.500964e-54	3.240193e-51
MsG0380014000.01	MsG0480021303.01	0.835290	1.828549e-56	2.226422e-53
MsG0380014000.01	MsG0480021420.01	0.826109	3.248629e-54	3.018207e-51
MsG0380014000.01	MsG0480021817.01	0.800998	1.107796e-48	5.232447e-46
MsG0380014000.01	MsG0480022804.01	0.821709	3.495872e-53	2.865875e-50
MsG0380014000.01	MsG0480023216.01	0.822258	2.607912e-53	2.171668e-50
MsG0380014000.01	MsG0480023395.01	0.812017	5.238820e-51	3.293133e-48
MsG0380014000.01	MsG0480023422.01	0.821365	4.197767e-53	3.408078e-50
MsG0380014000.01	MsG0480023881.01	0.805947	1.043591e-49	5.593931e-47
MsG0380014000.01	MsG0480023990.01	0.801961	7.032950e-49	3.403338e-46
MsG0380014000.01	MsG0580024567.01	0.820755	5.799366e-53	4.629293e-50
MsG0380014000.01	MsG0580024679.01	0.808595	2.867723e-50	1.646374e-47
MsG0380014000.01	MsG0580024763.01	0.807327	5.336145e-50	2.963640e-47
MsG0380014000.01	MsG0580024764.01	0.810721	1.001155e-50	6.080795e-48
MsG0380014000.01	MsG0580024782.01	-0.823291	1.498769e-53	1.285123e-50
MsG0380014000.01	MsG0580024873.01	0.817520	3.159561e-52	2.306194e-49
MsG0380014000.01	MsG0580024982.01	0.820097	8.212117e-53	6.437475e-50
MsG0380014000.01	MsG0580025589.01	0.823839	1.115928e-53	9.718481e-51
MsG0380014000.01	MsG0580026430.01	0.828812	7.300691e-55	7.335539e-52
MsG0380014000.01	MsG0580027098.01	0.852424	4.659981e-61	9.732248e-58
MsG0380014000.01	MsG0580027254.01	0.858461	8.108296e-63	2.071018e-59
MsG0380014000.01	MsG0580027899.01	0.805297	1.428797e-49	7.531789e-47
MsG0380014000.01	MsG0580028024.01	0.838554	2.683611e-57	3.607215e-54
MsG0380014000.01	MsG0580028353.01	0.824355	8.444588e-54	7.461538e-51
MsG0380014000.01	MsG0580028853.01	0.802142	6.456119e-49	3.138688e-46
MsG0380014000.01	MsG0580028925.01	0.814855	1.245652e-51	8.452538e-49
MsG0380014000.01	MsG0580029414.01	0.814836	1.257665e-51	8.529858e-49
MsG0380014000.01	MsG0580029719.01	0.827070	1.916626e-54	1.830501e-51
MsG0380014000.01	MsG0680032057.01	0.813649	2.299708e-51	1.510450e-48
MsG0380014000.01	MsG0680032515.01	0.807466	4.986897e-50	2.779747e-47
MsG0380014000.01	MsG0680032954.01	0.802241	6.159715e-49	3.002217e-46
MsG0380014000.01	MsG0680033966.01	0.810867	9.309315e-51	5.676397e-48
MsG0380014000.01	MsG0680034064.01	0.816660	4.930849e-52	3.515296e-49
MsG0380014000.01	MsG0780036387.01	0.800732	1.255432e-48	5.889342e-46
MsG0380014000.01	MsG0780037705.01	0.833494	5.162759e-56	5.955623e-53
MsG0380014000.01	MsG0780037915.01	0.830136	3.480136e-55	3.634812e-52
MsG0380014000.01	MsG0780037946.01	0.851657	7.694967e-61	1.568412e-57
MsG0380014000.01	MsG0780038571.01	0.820489	6.677615e-53	5.291020e-50
MsG0380014000.01	MsG0780038653.01	0.802576	5.255340e-49	2.583315e-46
MsG0380014000.01	MsG0780039707.01	0.829505	4.958526e-55	5.084684e-52
MsG0380014000.01	MsG0780039859.01	0.809945	1.472043e-50	8.759477e-48
MsG0380014000.01	MsG0780039975.01	0.828084	1.094475e-54	1.076717e-51
MsG0380014000.01	MsG0780040314.01	0.833639	4.751156e-56	5.504251e-53
MsG0380014000.01	MsG0780040315.01	0.827228	1.756261e-54	1.685162e-51
MsG0380014000.01	MsG0780040343.01	0.800104	1.685280e-48	7.780908e-46
MsG0380014000.01	MsG0780041004.01	0.850221	1.953997e-60	3.800575e-57
MsG0380014000.01	MsG0880042006.01	0.820426	6.903706e-53	5.460960e-50
MsG0380014000.01	MsG0880042290.01	0.810954	8.916752e-51	5.449963e-48
MsG0380014000.01	MsG0880042343.01	0.801764	7.718472e-49	3.716457e-46
MsG0380014000.01	MsG0880042931.01	0.808017	3.806794e-50	2.152654e-47
MsG0380014000.01	MsG0880043541.01	0.802976	4.343868e-49	2.157122e-46
MsG0380014000.01	MsG0880044314.01	0.815823	7.588893e-52	5.287535e-49
MsG0380014000.01	MsG0880044884.01	0.810236	1.274239e-50	7.640107e-48
MsG0380014000.01	MsG0880045283.01	0.819445	1.157167e-52	8.906585e-50
MsG0380014000.01	MsG0880045412.01	0.824944	6.132291e-54	5.511794e-51
MsG0380014000.01	MsG0880047156.01	0.801339	9.432981e-49	4.493648e-46
MsG0380014000.01	MsG0880047451.01	0.842049	3.277739e-58	4.908997e-55
MsG0280007041.01	MsG0380014000.01	0.825543	4.426801e-54	4.046927e-51
MsG0280009863.01	MsG0380014000.01	0.811534	6.671939e-51	4.140563e-48
MsG0280010198.01	MsG0380014000.01	0.815092	1.103712e-51	7.537375e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014000.01.T01	MTR_8g039420	74.468	47	12	0	21	67	9	55	2.92e-18	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 5 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGATGCTCAAGTAGAATT+TGG	0.211864	3:-48616826	MsG0380014000.01.T01:CDS
ACAGGCAGTCTGATAGAAAT+TGG	0.267535	3:-48616769	MsG0380014000.01.T01:CDS
TGCTGGTATTGATCCAGATT+TGG	0.356359	3:-48616845	MsG0380014000.01.T01:CDS
TCTACTTGAGCATCCAAATC+TGG	0.379441	3:+48616832	None:intergenic
GGTGCTAGAACCTTGTTCAG+AGG	0.595440	3:-48616872	MsG0380014000.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAAAAAGTCGAATCGAAA+AGG	+	Chr3:48616908-48616927	None:intergenic	30.0%
!	TTTGCTCTTCTCTTCTTTTT+CGG	-	Chr3:48616737-48616756	MsG0380014000.01.T01:CDS	30.0%
	GGAGATCAACTACAAAAAAC+AGG	-	Chr3:48616858-48616877	MsG0380014000.01.T01:CDS	35.0%
	TTGGATGCTCAAGTAGAATT+TGG	-	Chr3:48616819-48616838	MsG0380014000.01.T01:CDS	35.0%
!	GTAGTTGATCTCCAAGTTTT+TGG	+	Chr3:48616851-48616870	None:intergenic	35.0%
!	TTGCTCTTCTCTTCTTTTTC+GGG	-	Chr3:48616738-48616757	MsG0380014000.01.T01:CDS	35.0%
!	TTTGGAGCATTCCAAAAACT+TGG	-	Chr3:48616837-48616856	MsG0380014000.01.T01:CDS	35.0%
	ACAGGCAGTCTGATAGAAAT+TGG	-	Chr3:48616876-48616895	MsG0380014000.01.T01:CDS	40.0%
	TCTACTTGAGCATCCAAATC+TGG	+	Chr3:48616816-48616835	None:intergenic	40.0%
!!	TGCTGGTATTGATCCAGATT+TGG	-	Chr3:48616800-48616819	MsG0380014000.01.T01:CDS	40.0%
!!!	TCTTCTTTTTCGGGCACATT+TGG	-	Chr3:48616747-48616766	MsG0380014000.01.T01:CDS	40.0%
	CCAGCAAAAACCTCTGAACA+AGG	+	Chr3:48616786-48616805	None:intergenic	45.0%
!	CCTTGTTCAGAGGTTTTTGC+TGG	-	Chr3:48616783-48616802	MsG0380014000.01.T01:CDS	45.0%
!!!	TTTTTCGGGCACATTTGGCA+TGG	-	Chr3:48616752-48616771	MsG0380014000.01.T01:CDS	45.0%
	GGTGCTAGAACCTTGTTCAG+AGG	-	Chr3:48616773-48616792	MsG0380014000.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48616729	48616938	48616729	ID=MsG0380014000.01;Name=MsG0380014000.01
Chr3	mRNA	48616729	48616938	48616729	ID=MsG0380014000.01.T01;Parent=MsG0380014000.01;Name=MsG0380014000.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|69
Chr3	exon	48616729	48616938	48616729	ID=MsG0380014000.01.T01:exon:9427;Parent=MsG0380014000.01.T01
Chr3	CDS	48616729	48616938	48616729	ID=MsG0380014000.01.T01:cds;Parent=MsG0380014000.01.T01

Gene Sequence	>MsG0380014000.01.T01 ATGCTTCGTTTGCTCTTCTCTTCTTTTTCGGGCACATTTGGCATGGTGCTAGAACCTTGTTCAGAGGTTTTTGCTGGTATTGATCCAGATTTGGATGCTCAAGTAGAATTTGGAGCATTCCAAAAACTTGGAGATCAACTACAAAAAACAGGCAGTCTGATAGAAATTGGTTTGTTCCTTTTCGATTCGACTTTTTTTGTTGTTATTTGA
Protein sequence	>MsG0380014000.01.T01 MLRLLFSSFSGTFGMVLEPCSEVFAGIDPDLDAQVEFGAFQKLGDQLQKTGSLIEIGLFLFDSTFFVVI*