AlfalfaGEDB | Msa0187200

M. sativa cultivar zhongmu-4 / Msa0187200

KEGG: K03065
GO: GO:0000502 GO:0001678 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005838 GO:0005886 GO:0006355 GO:0006357 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008540 GO:0009056 GO:0009057 GO:0009553 GO:0009555 GO:0009743 GO:0009746 GO:0009749 GO:0009756 GO:0009757 GO:0009889 GO:0009891 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010033 GO:0010182 GO:0010243 GO:0010255 GO:0010468 GO:0010498 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017025 GO:0017111 GO:0019219 GO:0019222 GO:0019538 GO:0019725 GO:0019941 GO:0022624 GO:0023052 GO:0030162 GO:0030163 GO:0030433 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031331 GO:0031334 GO:0031595 GO:0031597 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0032991 GO:0033500 GO:0033554 GO:0034284 GO:0034976 GO:0036402 GO:0036503 GO:0042176 GO:0042221 GO:0042592 GO:0042593 GO:0042623 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043632 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0045732 GO:0045862 GO:0045893 GO:0045898 GO:0045899 GO:0045935 GO:0045944 GO:0048229 GO:0048518 GO:0048522 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051603 GO:0051716 GO:0055082 GO:0060255 GO:0060260 GO:0060261 GO:0061136 GO:0065007 GO:0065008 GO:0070013 GO:0070887 GO:0071310 GO:0071322 GO:0071326 GO:0071331 GO:0071333 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700 GO:1901701 GO:1901800 GO:1902494 GO:1902680 GO:1903050 GO:1903052 GO:1903362 GO:1903364 GO:1903506 GO:1903508 GO:1905368 GO:1905369 GO:2000112 GO:2000142 GO:2000144 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187200	MCI26402.1	89.231	65	7	0	1	65	1	65	3.74e-31	114

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187200	sp\|P54776\|PRS6A_SOLLC	76.190	63	15	0	1	63	1	63	1.80e-25	100

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187200	A0A392QRA4	89.231	65	7	0	1	65	1	65	1.79e-31	114

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002560	Msa0187200	0.849023	4.218951e-60	-8.615850e-47
Msa0003220	Msa0187200	0.823690	1.208944e-53	-8.615850e-47
Msa0010770	Msa0187200	0.810799	9.633025e-51	-8.615850e-47
Msa0041690	Msa0187200	0.807881	4.070360e-50	-8.615850e-47
Msa0054530	Msa0187200	0.805931	1.051850e-49	-8.615850e-47
Msa0064920	Msa0187200	0.813482	2.503813e-51	-8.615850e-47
Msa0074750	Msa0187200	0.813954	1.970134e-51	-8.615850e-47
Msa0078480	Msa0187200	0.800972	1.121690e-48	-8.615850e-47
Msa0091780	Msa0187200	0.810685	1.019388e-50	-8.615850e-47
Msa0106620	Msa0187200	0.807984	3.870291e-50	-8.615850e-47
Msa0126230	Msa0187200	0.800961	1.127502e-48	-8.615850e-47
Msa0131700	Msa0187200	0.833286	5.817545e-56	-8.615850e-47
Msa0138210	Msa0187200	0.806375	8.480268e-50	-8.615850e-47
Msa0143850	Msa0187200	0.829539	4.865754e-55	-8.615850e-47
Msa0144780	Msa0187200	0.802763	4.807513e-49	-8.615850e-47
Msa0183420	Msa0187200	0.813920	2.005167e-51	-8.615850e-47
Msa0187200	Msa0236770	0.808854	2.524420e-50	-8.615850e-47
Msa0187200	Msa0237200	0.801145	1.033673e-48	-8.615850e-47
Msa0187200	Msa0358400	0.806316	8.728028e-50	-8.615850e-47
Msa0187200	Msa0363060	0.808845	2.535432e-50	-8.615850e-47
Msa0187200	Msa0376020	0.841529	4.496442e-58	-8.615850e-47
Msa0187200	Msa0376280	0.809898	1.506650e-50	-8.615850e-47
Msa0187200	Msa0381000	0.803077	4.139647e-49	-8.615850e-47
Msa0187200	Msa0387050	0.819933	8.953889e-53	-8.615850e-47
Msa0187200	Msa0394690	0.815922	7.211753e-52	-8.615850e-47
Msa0187200	Msa0423840	0.808330	3.265284e-50	-8.615850e-47
Msa0187200	Msa0430870	0.803311	3.703615e-49	-8.615850e-47
Msa0187200	Msa0433610	0.808382	3.183330e-50	-8.615850e-47
Msa0187200	Msa0446900	0.805732	1.158376e-49	-8.615850e-47
Msa0187200	Msa0468250	0.814909	1.211796e-51	-8.615850e-47
Msa0187200	Msa0516020	0.842924	1.920711e-58	-8.615850e-47
Msa0187200	Msa0516120	0.846012	2.837098e-59	-8.615850e-47
Msa0187200	Msa0624120	0.803694	3.085434e-49	-8.615850e-47
Msa0187200	Msa0679100	0.806942	6.436627e-50	-8.615850e-47
Msa0187200	Msa0689760	0.808008	3.825064e-50	-8.615850e-47
Msa0187200	Msa0698100	0.808287	3.335857e-50	-8.615850e-47
Msa0187200	Msa0743530	0.822487	2.307946e-53	-8.615850e-47
Msa0187200	Msa0749400	0.809550	1.790567e-50	-8.615850e-47
Msa0187200	Msa0788110	0.811352	7.307694e-51	-8.615850e-47
Msa0187200	Msa0789560	0.820150	7.982978e-53	-8.615850e-47
Msa0187200	Msa0820890	0.800054	1.725567e-48	-8.615850e-47
Msa0187200	Msa0870650	0.806787	6.942068e-50	-8.615850e-47
Msa0187200	Msa0969330	0.805102	1.569721e-49	-8.615850e-47
Msa0187200	Msa0996020	0.807594	4.683072e-50	-8.615850e-47
Msa0187200	Msa1077070	0.825326	4.982185e-54	-8.615850e-47
Msa0187200	Msa1095790	0.812118	4.979382e-51	-8.615850e-47
Msa0187200	Msa1098890	0.827435	1.567110e-54	-8.615850e-47
Msa0187200	Msa1122780	0.829815	4.167457e-55	-8.615850e-47
Msa0187200	Msa1127430	0.802574	5.258533e-49	-8.615850e-47
Msa0187200	Msa1168590	0.802611	5.167893e-49	-8.615850e-47
Msa0187200	Msa1201640	0.844384	7.818574e-59	-8.615850e-47
Msa0187200	Msa1211340	0.809790	1.589909e-50	-8.615850e-47
Msa0187200	Msa1215070	0.808656	2.782417e-50	-8.615850e-47
Msa0187200	Msa1254620	0.817566	3.085950e-52	-8.615850e-47
Msa0187200	Msa1259640	0.809284	2.042190e-50	-8.615850e-47
Msa0187200	Msa1299330	0.812343	4.447685e-51	-8.615850e-47
Msa0187200	Msa1320820	0.811754	5.977521e-51	-8.615850e-47
Msa0187200	Msa1329790	0.817377	3.403356e-52	-8.615850e-47
Msa0187200	Msa1336040	0.801243	9.871072e-49	-8.615850e-47
Msa0187200	Msa1341490	0.807052	6.102033e-50	-8.615850e-47
Msa0187200	Msa1364430	0.821035	4.999875e-53	-8.615850e-47
Msa0187200	Msa1409950	0.803236	3.837617e-49	-8.615850e-47
Msa0187200	Msa1416260	0.800061	1.719470e-48	-8.615850e-47
Msa0187200	Msa1420250	0.802721	4.905133e-49	-8.615850e-47
Msa0187200	Msa1426910	0.806464	8.121600e-50	-8.615850e-47
Msa0187200	Msa1448490	0.832672	8.272415e-56	-8.615850e-47
Msa0187200	Msa1450720	0.802469	5.528376e-49	-8.615850e-47
Msa0187200	Msa1467000	0.808576	2.893478e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187200	MtrunA17_Chr1g0192151	95.238	63	3	0	1	63	1	63	4.99e-34	119
Msa0187200	MtrunA17_Chr4g0014731	90.164	61	6	0	3	63	4	64	8.38e-31	111

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0187200	AT1G09100.1	77.778	63	14	0	1	63	1	63	7.15e-26	98.2
Msa0187200	AT3G05530.1	73.438	64	16	1	1	63	1	64	2.04e-24	94.4

Find 9 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGATTTAGACAACTGTTTC+AGG	0.255465	2_1:+1899016	Msa0187200:CDS
AGAGAAGATTAAAGAGAATC+AGG	0.321169	2_1:+1898972	Msa0187200:CDS
CGGTGCTCATATTCGCTAAT+TGG	0.368891	2_1:-1898737	None:intergenic
AGAGTCGCAGAGGACGAATC+TGG	0.398430	2_1:+1898933	Msa0187200:CDS
GCAGAGGACGAATCTGGAGT+TGG	0.554081	2_1:+1898939	Msa0187200:CDS
CAACGAGATTCGCATCCTCA+AGG	0.561580	2_1:+1898791	Msa0187200:CDS
AGAAGCTCTGACAATATCGT+CGG	0.672009	2_1:-1898757	None:intergenic
AATCGATATGGTGAGTGCAA+TGG	0.678296	2_1:+1898689	None:intergenic
TGATGTAGGAAGAGTCGCAG+AGG	0.711080	2_1:+1898923	Msa0187200:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTGTATTATTGATGT+AGG	+	chr2_1:1898909-1898928	Msa0187200:intron	15.0%
!!	TTATGTAACTTTGTATTGTT+AGG	+	chr2_1:1898849-1898868	Msa0187200:intron	20.0%
!!	TATGTAACTTTGTATTGTTA+GGG	+	chr2_1:1898850-1898869	Msa0187200:intron	20.0%
!	GAATAAAGAGAGAAGAAAAA+AGG	-	chr2_1:1898819-1898838	None:intergenic	25.0%
!	CAAAAAATTCAACCAAAATC+AGG	-	chr2_1:1898897-1898916	None:intergenic	25.0%
!	TTTTGTTGAGTTCCTGATTT+TGG	+	chr2_1:1898882-1898901	Msa0187200:intron	30.0%
	AGAGAAGATTAAAGAGAATC+AGG	+	chr2_1:1898972-1898991	Msa0187200:CDS	30.0%
	TTGATTTAGACAACTGTTTC+AGG	+	chr2_1:1899016-1899035	Msa0187200:CDS	30.0%
	AGAAGAAAAAAGGAACCTTG+AGG	-	chr2_1:1898809-1898828	None:intergenic	35.0%
!	AGAAGCTCTGACAATATCGT+CGG	-	chr2_1:1898760-1898779	None:intergenic	40.0%
	CGGTGCTCATATTCGCTAAT+TGG	-	chr2_1:1898740-1898759	None:intergenic	45.0%
	CAACGAGATTCGCATCCTCA+AGG	+	chr2_1:1898791-1898810	Msa0187200:CDS	50.0%
	TGATGTAGGAAGAGTCGCAG+AGG	+	chr2_1:1898923-1898942	Msa0187200:intron	50.0%
	AGAGTCGCAGAGGACGAATC+TGG	+	chr2_1:1898933-1898952	Msa0187200:CDS	55.0%
!	GCAGAGGACGAATCTGGAGT+TGG	+	chr2_1:1898939-1898958	Msa0187200:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	1898696	1899044	1898696	ID=Msa0187200;Name=Msa0187200
chr2_1	mRNA	1898696	1899044	1898696	ID=Msa0187200-mRNA-1;Parent=Msa0187200;Name=Msa0187200-mRNA-1;_AED=0.72;_eAED=0.72;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|76
chr2_1	exon	1898696	1898812	1898696	ID=Msa0187200-mRNA-1:exon:900;Parent=Msa0187200-mRNA-1
chr2_1	exon	1898931	1899044	1898931	ID=Msa0187200-mRNA-1:exon:901;Parent=Msa0187200-mRNA-1
chr2_1	CDS	1898696	1898812	1898696	ID=Msa0187200-mRNA-1:cds;Parent=Msa0187200-mRNA-1
chr2_1	CDS	1898931	1899044	1898931	ID=Msa0187200-mRNA-1:cds;Parent=Msa0187200-mRNA-1

Gene Sequence	>Msa0187200 ATGGTGAGTGCAATGGTAGAAGATGCTAACTTCGAAGATGACCAATTAGCGAATATGAGCACCGACGATATTGTCAGAGCTTCTCGTCTTCTCGACAACGAGATTCGCATCCTCAAGGAAGAGTCGCAGAGGACGAATCTGGAGTTGGAATCGTATAAAGAGAAGATTAAAGAGAATCAGGAGAACTTTAGTTGCAAAATGTTTGATTTAGACAACTGTTTCAGGACATGA
Protein sequence	>Msa0187200 MVSAMVEDANFEDDQLANMSTDDIVRASRLLDNEIRILKEESQRTNLELESYKEKIKENQENFSCKMFDLDNCFRT*