AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0199520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0199520 MtrunA17_Chr2g0293951 89.394 66 6 1 1 65 1 66 2.43e-37 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 54 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCATATCCACTGTATTAT+TGG 0.142549 2_1:-18662702 Msa0199520:three_prime_UTR
GCCATTTGTGCAATAATTAA+TGG 0.157220 2_1:-18663675 Msa0199520:three_prime_UTR
TCCTGTTAAGGCAATGAATT+TGG 0.172129 2_1:-18662888 Msa0199520:three_prime_UTR
ATAATTAATGGCTTGAAAAT+TGG 0.202907 2_1:-18663663 Msa0199520:three_prime_UTR
TAGAGATACTATTGTTAAAT+AGG 0.221511 2_1:-18663076 Msa0199520:three_prime_UTR
TATTGCACAAATGGCGAAAA+TGG 0.236264 2_1:+18663683 None:intergenic
ATGCATGAGATGTTCCTTAT+TGG 0.244420 2_1:+18664109 None:intergenic
GCCAAATTCATTGCCTTAAC+AGG 0.245227 2_1:+18662887 None:intergenic
AGAATGAGTTGGGTGGTAAA+AGG 0.270914 2_1:+18663738 None:intergenic
TTATTAAATAAATAAATAAA+AGG 0.275241 2_1:+18662623 None:intergenic
ATATCAATTTATTAGTTATA+TGG 0.287568 2_1:+18663138 None:intergenic
AATATTTATGCTGTTGTTGC+AGG 0.303257 2_1:-18662839 Msa0199520:three_prime_UTR
ATTAATTTAATTATTAAGTA+TGG 0.307174 2_1:+18662960 None:intergenic
GCAAGTTGTAATGTGCAAAT+TGG 0.320667 2_1:-18663623 Msa0199520:three_prime_UTR
TGCATGAGATGTTCCTTATT+GGG 0.359374 2_1:+18664110 None:intergenic
CCTTTGACAAATTTGTCTTT+TGG 0.367129 2_1:+18664485 None:intergenic
GCCATTAATTATTGCACAAA+TGG 0.388761 2_1:+18663674 None:intergenic
ATAATACAGTGGATATGAAT+TGG 0.419756 2_1:+18662705 None:intergenic
CTTTCTCCACATAATAATAG+AGG 0.437887 2_1:-18662542 None:intergenic
ATATGATACAGGTCCCAATA+AGG 0.444359 2_1:-18664123 Msa0199520:intron
GATACTATTGTTAAATAGGT+AGG 0.445055 2_1:-18663072 Msa0199520:three_prime_UTR
GCAACTTCAGCATTAGTAGC+AGG 0.456113 2_1:-18663975 Msa0199520:CDS
AGATATTATATAATATATGC+AGG 0.457860 2_1:+18663223 None:intergenic
ATTTGTATGATATCCTGTTA+AGG 0.463224 2_1:-18662900 Msa0199520:three_prime_UTR
TCTGAATTTCTAAAAGAATG+AGG 0.464548 2_1:+18662660 None:intergenic
AAGAAAAGAGAGAATGAGTT+GGG 0.471821 2_1:+18663728 None:intergenic
CTGAATTTCTAAAAGAATGA+GGG 0.473117 2_1:+18662661 None:intergenic
AAATTTACTATAGTACTAGA+AGG 0.474748 2_1:-18663001 Msa0199520:three_prime_UTR
GAATGAGTTGGGTGGTAAAA+GGG 0.477567 2_1:+18663739 None:intergenic
CTCTATTATTATGTGGAGAA+AGG 0.479861 2_1:+18662543 None:intergenic
CAGGTTCATTCTAATCATCA+TGG 0.481892 2_1:-18663956 Msa0199520:exon
CAAGAAAAGAGAGAATGAGT+TGG 0.487817 2_1:+18663727 None:intergenic
GTTGGAGCAATTTAAAGTGT+AGG 0.488712 2_1:-18663439 Msa0199520:three_prime_UTR
GGCGAAAATGGAACTAGCTC+AGG 0.495694 2_1:+18663695 None:intergenic
GGTCTTACAAGGTCAAGAAA+AGG 0.544806 2_1:+18663848 None:intergenic
GATATTATATAATATATGCA+GGG 0.547921 2_1:+18663224 None:intergenic
TTTGCACATTACAACTTGCA+AGG 0.561682 2_1:+18663627 None:intergenic
GTGAAACCTCTATTATTATG+TGG 0.567151 2_1:+18662536 None:intergenic
GGTCAAGAAAAGGTCTTACA+AGG 0.571310 2_1:+18663858 None:intergenic
ACACAGAGTGAGGTCTTACA+AGG 0.589940 2_1:+18663837 None:intergenic
CCAAAAGACAAATTTGTCAA+AGG 0.590749 2_1:-18664485 Msa0199520:intron
GAAAATGGAACTAGCTCAGG+AGG 0.596315 2_1:+18663698 None:intergenic
AGGTTCATTCTAATCATCAT+GGG 0.608536 2_1:-18663955 Msa0199520:exon
TGTAAGACCTCACTCTGTGT+CGG 0.612142 2_1:-18663834 Msa0199520:three_prime_UTR
GTGGTGATGAACTTGTTGCT+AGG 0.619962 2_1:+18664077 None:intergenic
AAAAGAGAGAATGAGTTGGG+TGG 0.659299 2_1:+18663731 None:intergenic
ATATTATATAATATATGCAG+GGG 0.670495 2_1:+18663225 None:intergenic
TATCAACTGAATGAACCTCA+CGG 0.674472 2_1:-18663199 Msa0199520:three_prime_UTR
GAATCTCAAAGGGTTGACCA+TGG 0.676450 2_1:+18664055 None:intergenic
AAGAAAACCGACACAGAGTG+AGG 0.678747 2_1:+18663827 None:intergenic
TCTCAAAGGGTTGACCATGG+TGG 0.686799 2_1:+18664058 None:intergenic
AGATAAGTCCAATAATACAG+TGG 0.698868 2_1:+18662694 None:intergenic
AAAATGGAACTAGCTCAGGA+GGG 0.709909 2_1:+18663699 None:intergenic
CAACAAGTTCATCACCACCA+TGG 0.713697 2_1:-18664072 Msa0199520:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTAAATAAATAAATAAA+AGG + chr2_1:18664439-18664458 None:intergenic 0.0%
!! TTATTAAATAAATAAATAAA+AGG + chr2_1:18664439-18664458 None:intergenic 0.0%
!! ATTAATTTAGCATATAAAAA+AGG + chr2_1:18662671-18662690 None:intergenic 10.0%
!! GTATATTATAATATAAAGTA+TGG + chr2_1:18662754-18662773 None:intergenic 10.0%
!!! ATTCTTTAATGTTTTTTAAT+TGG - chr2_1:18662837-18662856 Msa0199520:three_prime_UTR 10.0%
!! ATATCAATTTATTAGTTATA+TGG + chr2_1:18663924-18663943 None:intergenic 10.0%
!!! TTTTTTGAACTAATAAATAT+AGG + chr2_1:18664028-18664047 None:intergenic 10.0%
!! ATTAATTTAGCATATAAAAA+AGG + chr2_1:18662671-18662690 None:intergenic 10.0%
!! GTATATTATAATATAAAGTA+TGG + chr2_1:18662754-18662773 None:intergenic 10.0%
!!! ATTCTTTAATGTTTTTTAAT+TGG - chr2_1:18662837-18662856 Msa0199520:three_prime_UTR 10.0%
!! ATATCAATTTATTAGTTATA+TGG + chr2_1:18663924-18663943 None:intergenic 10.0%
!!! TTTTTTGAACTAATAAATAT+AGG + chr2_1:18664028-18664047 None:intergenic 10.0%
!! ATATGCTAAATTAATGTTAA+AGG - chr2_1:18662676-18662695 Msa0199520:three_prime_UTR 15.0%
!!! TTGTTTATATTTTATTGTGA+AGG + chr2_1:18663553-18663572 None:intergenic 15.0%
!! ATCATATTAAAAAAATGTAG+TGG - chr2_1:18663696-18663715 Msa0199520:three_prime_UTR 15.0%
!! ATATTATATAATATATGCAG+GGG + chr2_1:18663837-18663856 None:intergenic 15.0%
!! GATATTATATAATATATGCA+GGG + chr2_1:18663838-18663857 None:intergenic 15.0%
!! AGATATTATATAATATATGC+AGG + chr2_1:18663839-18663858 None:intergenic 15.0%
!! ATATGCTAAATTAATGTTAA+AGG - chr2_1:18662676-18662695 Msa0199520:three_prime_UTR 15.0%
!!! TTGTTTATATTTTATTGTGA+AGG + chr2_1:18663553-18663572 None:intergenic 15.0%
!! ATCATATTAAAAAAATGTAG+TGG - chr2_1:18663696-18663715 Msa0199520:three_prime_UTR 15.0%
!! ATATTATATAATATATGCAG+GGG + chr2_1:18663837-18663856 None:intergenic 15.0%
!! GATATTATATAATATATGCA+GGG + chr2_1:18663838-18663857 None:intergenic 15.0%
!! AGATATTATATAATATATGC+AGG + chr2_1:18663839-18663858 None:intergenic 15.0%
!! TGATGAAAGTAAAATGATTA+TGG + chr2_1:18662630-18662649 None:intergenic 20.0%
!! AAAAAGTTAAGAAAAGAGAA+AGG + chr2_1:18663157-18663176 None:intergenic 20.0%
!! ATAATTAATGGCTTGAAAAT+TGG - chr2_1:18663396-18663415 Msa0199520:three_prime_UTR 20.0%
!! TTGAAAATTGGATGAAAAAA+AGG - chr2_1:18663408-18663427 Msa0199520:three_prime_UTR 20.0%
!!! ATTTTATTGTGAAGGATATT+AGG + chr2_1:18663545-18663564 None:intergenic 20.0%
!!! ACAATTCACTTTTATTTTGT+TGG - chr2_1:18663602-18663621 Msa0199520:three_prime_UTR 20.0%
!! TAGAGATACTATTGTTAAAT+AGG - chr2_1:18663983-18664002 Msa0199520:CDS 20.0%
!! TATTAGTTCAAAAAAACATG+AGG - chr2_1:18664033-18664052 Msa0199520:CDS 20.0%
!! AAATTTACTATAGTACTAGA+AGG - chr2_1:18664058-18664077 Msa0199520:CDS 20.0%
!!! TAGTTGTTTTATATGTTGTT+TGG - chr2_1:18664252-18664271 Msa0199520:intron 20.0%
!! TGATGAAAGTAAAATGATTA+TGG + chr2_1:18662630-18662649 None:intergenic 20.0%
!! AAAAAGTTAAGAAAAGAGAA+AGG + chr2_1:18663157-18663176 None:intergenic 20.0%
!! ATAATTAATGGCTTGAAAAT+TGG - chr2_1:18663396-18663415 Msa0199520:three_prime_UTR 20.0%
!! TTGAAAATTGGATGAAAAAA+AGG - chr2_1:18663408-18663427 Msa0199520:three_prime_UTR 20.0%
!!! ATTTTATTGTGAAGGATATT+AGG + chr2_1:18663545-18663564 None:intergenic 20.0%
!!! ACAATTCACTTTTATTTTGT+TGG - chr2_1:18663602-18663621 Msa0199520:three_prime_UTR 20.0%
!! TAGAGATACTATTGTTAAAT+AGG - chr2_1:18663983-18664002 Msa0199520:CDS 20.0%
!! TATTAGTTCAAAAAAACATG+AGG - chr2_1:18664033-18664052 Msa0199520:CDS 20.0%
!! AAATTTACTATAGTACTAGA+AGG - chr2_1:18664058-18664077 Msa0199520:CDS 20.0%
!!! TAGTTGTTTTATATGTTGTT+TGG - chr2_1:18664252-18664271 Msa0199520:intron 20.0%
!! ATTGGATTTAAATCTAAAGC+TGG - chr2_1:18662855-18662874 Msa0199520:three_prime_UTR 25.0%
! GATACTATTGTTAAATAGGT+AGG - chr2_1:18663987-18664006 Msa0199520:CDS 25.0%
! ATTTGTATGATATCCTGTTA+AGG - chr2_1:18664159-18664178 Msa0199520:intron 25.0%
! ATAATACAGTGGATATGAAT+TGG + chr2_1:18664357-18664376 None:intergenic 25.0%
! ATTCATATCCACTGTATTAT+TGG - chr2_1:18664357-18664376 Msa0199520:intron 25.0%
!! CTGAATTTCTAAAAGAATGA+GGG + chr2_1:18664401-18664420 None:intergenic 25.0%
!! TCTGAATTTCTAAAAGAATG+AGG + chr2_1:18664402-18664421 None:intergenic 25.0%
!! ATTGGATTTAAATCTAAAGC+TGG - chr2_1:18662855-18662874 Msa0199520:three_prime_UTR 25.0%
! GATACTATTGTTAAATAGGT+AGG - chr2_1:18663987-18664006 Msa0199520:CDS 25.0%
! ATTTGTATGATATCCTGTTA+AGG - chr2_1:18664159-18664178 Msa0199520:intron 25.0%
! ATAATACAGTGGATATGAAT+TGG + chr2_1:18664357-18664376 None:intergenic 25.0%
! ATTCATATCCACTGTATTAT+TGG - chr2_1:18664357-18664376 Msa0199520:intron 25.0%
!! CTGAATTTCTAAAAGAATGA+GGG + chr2_1:18664401-18664420 None:intergenic 25.0%
!! TCTGAATTTCTAAAAGAATG+AGG + chr2_1:18664402-18664421 None:intergenic 25.0%
! CCTTTGACAAATTTGTCTTT+TGG + chr2_1:18662577-18662596 None:intergenic 30.0%
CCAAAAGACAAATTTGTCAA+AGG - chr2_1:18662574-18662593 Msa0199520:three_prime_UTR 30.0%
! TTTTGTCACAGAATCTCAAA+GGG + chr2_1:18663017-18663036 None:intergenic 30.0%
! TTTTTGTCACAGAATCTCAA+AGG + chr2_1:18663018-18663037 None:intergenic 30.0%
GACAAAAAAGAAGAAGACAT+CGG - chr2_1:18663030-18663049 Msa0199520:three_prime_UTR 30.0%
AGGTTCATTCTAATCATCAT+GGG - chr2_1:18663104-18663123 Msa0199520:three_prime_UTR 30.0%
!! TTTCTTCGTTTTTGCTTTTG+TGG - chr2_1:18663249-18663268 Msa0199520:three_prime_UTR 30.0%
AAGAAAAGAGAGAATGAGTT+GGG + chr2_1:18663334-18663353 None:intergenic 30.0%
GCCATTTGTGCAATAATTAA+TGG - chr2_1:18663384-18663403 Msa0199520:three_prime_UTR 30.0%
GCCATTAATTATTGCACAAA+TGG + chr2_1:18663388-18663407 None:intergenic 30.0%
AATATTTATGCTGTTGTTGC+AGG - chr2_1:18664220-18664239 Msa0199520:intron 30.0%
AGATAAGTCCAATAATACAG+TGG + chr2_1:18664368-18664387 None:intergenic 30.0%
! CCTTTGACAAATTTGTCTTT+TGG + chr2_1:18662577-18662596 None:intergenic 30.0%
CCAAAAGACAAATTTGTCAA+AGG - chr2_1:18662574-18662593 Msa0199520:three_prime_UTR 30.0%
! TTTTGTCACAGAATCTCAAA+GGG + chr2_1:18663017-18663036 None:intergenic 30.0%
! TTTTTGTCACAGAATCTCAA+AGG + chr2_1:18663018-18663037 None:intergenic 30.0%
GACAAAAAAGAAGAAGACAT+CGG - chr2_1:18663030-18663049 Msa0199520:three_prime_UTR 30.0%
AGGTTCATTCTAATCATCAT+GGG - chr2_1:18663104-18663123 Msa0199520:three_prime_UTR 30.0%
!! TTTCTTCGTTTTTGCTTTTG+TGG - chr2_1:18663249-18663268 Msa0199520:three_prime_UTR 30.0%
AAGAAAAGAGAGAATGAGTT+GGG + chr2_1:18663334-18663353 None:intergenic 30.0%
GCCATTTGTGCAATAATTAA+TGG - chr2_1:18663384-18663403 Msa0199520:three_prime_UTR 30.0%
GCCATTAATTATTGCACAAA+TGG + chr2_1:18663388-18663407 None:intergenic 30.0%
AATATTTATGCTGTTGTTGC+AGG - chr2_1:18664220-18664239 Msa0199520:intron 30.0%
AGATAAGTCCAATAATACAG+TGG + chr2_1:18664368-18664387 None:intergenic 30.0%
! ACTACTAGACTTGTTCAATG+TGG - chr2_1:18662598-18662617 Msa0199520:three_prime_UTR 35.0%
TGCAGAGAATGAAGAAAGAA+TGG + chr2_1:18662893-18662912 None:intergenic 35.0%
GAGCATGATTCATATGATAC+AGG - chr2_1:18662925-18662944 Msa0199520:three_prime_UTR 35.0%
ATATGATACAGGTCCCAATA+AGG - chr2_1:18662936-18662955 Msa0199520:three_prime_UTR 35.0%
TGCATGAGATGTTCCTTATT+GGG + chr2_1:18662952-18662971 None:intergenic 35.0%
ATGCATGAGATGTTCCTTAT+TGG + chr2_1:18662953-18662972 None:intergenic 35.0%
AAAAAAGAAGAAGACATCGG+TGG - chr2_1:18663033-18663052 Msa0199520:three_prime_UTR 35.0%
CAGGTTCATTCTAATCATCA+TGG - chr2_1:18663103-18663122 Msa0199520:three_prime_UTR 35.0%
GGTGAAAAAAAAGCTCAGAT+AGG + chr2_1:18663136-18663155 None:intergenic 35.0%
CAAGAAAAGAGAGAATGAGT+TGG + chr2_1:18663335-18663354 None:intergenic 35.0%
TATTGCACAAATGGCGAAAA+TGG + chr2_1:18663379-18663398 None:intergenic 35.0%
TTTGCACATTACAACTTGCA+AGG + chr2_1:18663435-18663454 None:intergenic 35.0%
GCAAGTTGTAATGTGCAAAT+TGG - chr2_1:18663436-18663455 Msa0199520:three_prime_UTR 35.0%
! GTTGGAGCAATTTAAAGTGT+AGG - chr2_1:18663620-18663639 Msa0199520:three_prime_UTR 35.0%
TATCAACTGAATGAACCTCA+CGG - chr2_1:18663860-18663879 Msa0199520:three_prime_UTR 35.0%
! AAGTTTTTATTGTCTCCGTG+AGG + chr2_1:18663878-18663897 None:intergenic 35.0%
TCCTGTTAAGGCAATGAATT+TGG - chr2_1:18664171-18664190 Msa0199520:intron 35.0%
! ACTACTAGACTTGTTCAATG+TGG - chr2_1:18662598-18662617 Msa0199520:three_prime_UTR 35.0%
TGCAGAGAATGAAGAAAGAA+TGG + chr2_1:18662893-18662912 None:intergenic 35.0%
GAGCATGATTCATATGATAC+AGG - chr2_1:18662925-18662944 Msa0199520:three_prime_UTR 35.0%
ATATGATACAGGTCCCAATA+AGG - chr2_1:18662936-18662955 Msa0199520:three_prime_UTR 35.0%
TGCATGAGATGTTCCTTATT+GGG + chr2_1:18662952-18662971 None:intergenic 35.0%
ATGCATGAGATGTTCCTTAT+TGG + chr2_1:18662953-18662972 None:intergenic 35.0%
AAAAAAGAAGAAGACATCGG+TGG - chr2_1:18663033-18663052 Msa0199520:three_prime_UTR 35.0%
CAGGTTCATTCTAATCATCA+TGG - chr2_1:18663103-18663122 Msa0199520:three_prime_UTR 35.0%
GGTGAAAAAAAAGCTCAGAT+AGG + chr2_1:18663136-18663155 None:intergenic 35.0%
CAAGAAAAGAGAGAATGAGT+TGG + chr2_1:18663335-18663354 None:intergenic 35.0%
TATTGCACAAATGGCGAAAA+TGG + chr2_1:18663379-18663398 None:intergenic 35.0%
TTTGCACATTACAACTTGCA+AGG + chr2_1:18663435-18663454 None:intergenic 35.0%
GCAAGTTGTAATGTGCAAAT+TGG - chr2_1:18663436-18663455 Msa0199520:three_prime_UTR 35.0%
! GTTGGAGCAATTTAAAGTGT+AGG - chr2_1:18663620-18663639 Msa0199520:three_prime_UTR 35.0%
TATCAACTGAATGAACCTCA+CGG - chr2_1:18663860-18663879 Msa0199520:three_prime_UTR 35.0%
! AAGTTTTTATTGTCTCCGTG+AGG + chr2_1:18663878-18663897 None:intergenic 35.0%
TCCTGTTAAGGCAATGAATT+TGG - chr2_1:18664171-18664190 Msa0199520:intron 35.0%
GAGAATGAAGAAAGAATGGC+AGG + chr2_1:18662889-18662908 None:intergenic 40.0%
GGTCAAGAAAAGGTCTTACA+AGG + chr2_1:18663204-18663223 None:intergenic 40.0%
GGTCTTACAAGGTCAAGAAA+AGG + chr2_1:18663214-18663233 None:intergenic 40.0%
TGGGTGGTAAAAGGGAAAAA+CGG + chr2_1:18663315-18663334 None:intergenic 40.0%
! GAATGAGTTGGGTGGTAAAA+GGG + chr2_1:18663323-18663342 None:intergenic 40.0%
! AGAATGAGTTGGGTGGTAAA+AGG + chr2_1:18663324-18663343 None:intergenic 40.0%
! AAAAGAGAGAATGAGTTGGG+TGG + chr2_1:18663331-18663350 None:intergenic 40.0%
AAAATGGAACTAGCTCAGGA+GGG + chr2_1:18663363-18663382 None:intergenic 40.0%
GCCAAATTCATTGCCTTAAC+AGG + chr2_1:18664175-18664194 None:intergenic 40.0%
GAGAATGAAGAAAGAATGGC+AGG + chr2_1:18662889-18662908 None:intergenic 40.0%
GGTCAAGAAAAGGTCTTACA+AGG + chr2_1:18663204-18663223 None:intergenic 40.0%
GGTCTTACAAGGTCAAGAAA+AGG + chr2_1:18663214-18663233 None:intergenic 40.0%
TGGGTGGTAAAAGGGAAAAA+CGG + chr2_1:18663315-18663334 None:intergenic 40.0%
! GAATGAGTTGGGTGGTAAAA+GGG + chr2_1:18663323-18663342 None:intergenic 40.0%
! AGAATGAGTTGGGTGGTAAA+AGG + chr2_1:18663324-18663343 None:intergenic 40.0%
! AAAAGAGAGAATGAGTTGGG+TGG + chr2_1:18663331-18663350 None:intergenic 40.0%
AAAATGGAACTAGCTCAGGA+GGG + chr2_1:18663363-18663382 None:intergenic 40.0%
GCCAAATTCATTGCCTTAAC+AGG + chr2_1:18664175-18664194 None:intergenic 40.0%
!! GTGGTGATGAACTTGTTGCT+AGG + chr2_1:18662985-18663004 None:intergenic 45.0%
CAACAAGTTCATCACCACCA+TGG - chr2_1:18662987-18663006 Msa0199520:three_prime_UTR 45.0%
GAATCTCAAAGGGTTGACCA+TGG + chr2_1:18663007-18663026 None:intergenic 45.0%
GCAACTTCAGCATTAGTAGC+AGG - chr2_1:18663084-18663103 Msa0199520:three_prime_UTR 45.0%
ACACAGAGTGAGGTCTTACA+AGG + chr2_1:18663225-18663244 None:intergenic 45.0%
TGTAAGACCTCACTCTGTGT+CGG - chr2_1:18663225-18663244 Msa0199520:three_prime_UTR 45.0%
AAGAAAACCGACACAGAGTG+AGG + chr2_1:18663235-18663254 None:intergenic 45.0%
GAAAATGGAACTAGCTCAGG+AGG + chr2_1:18663364-18663383 None:intergenic 45.0%
!! GTGGTGATGAACTTGTTGCT+AGG + chr2_1:18662985-18663004 None:intergenic 45.0%
CAACAAGTTCATCACCACCA+TGG - chr2_1:18662987-18663006 Msa0199520:three_prime_UTR 45.0%
GAATCTCAAAGGGTTGACCA+TGG + chr2_1:18663007-18663026 None:intergenic 45.0%
GCAACTTCAGCATTAGTAGC+AGG - chr2_1:18663084-18663103 Msa0199520:three_prime_UTR 45.0%
ACACAGAGTGAGGTCTTACA+AGG + chr2_1:18663225-18663244 None:intergenic 45.0%
TGTAAGACCTCACTCTGTGT+CGG - chr2_1:18663225-18663244 Msa0199520:three_prime_UTR 45.0%
AAGAAAACCGACACAGAGTG+AGG + chr2_1:18663235-18663254 None:intergenic 45.0%
GAAAATGGAACTAGCTCAGG+AGG + chr2_1:18663364-18663383 None:intergenic 45.0%
!! ATTAATTTAATTATTAAGTA+TGG + chr2_1:18664102-18664121 None:intergenic 5.0%
!! ATTAATTTAATTATTAAGTA+TGG + chr2_1:18664102-18664121 None:intergenic 5.0%
TCTCAAAGGGTTGACCATGG+TGG + chr2_1:18663004-18663023 None:intergenic 50.0%
GGCGAAAATGGAACTAGCTC+AGG + chr2_1:18663367-18663386 None:intergenic 50.0%
TCTCAAAGGGTTGACCATGG+TGG + chr2_1:18663004-18663023 None:intergenic 50.0%
GGCGAAAATGGAACTAGCTC+AGG + chr2_1:18663367-18663386 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 18662545 18664536 18662545 ID=Msa0199520;Name=Msa0199520
chr2_1 mRNA 18662545 18664536 18662545 ID=Msa0199520-mRNA-1;Parent=Msa0199520;Name=Msa0199520-mRNA-1;_AED=0.00;_eAED=0.00;_QI=23|1|1|1|1|1|2|1420|65
chr2_1 exon 18662545 18664134 18662545 ID=Msa0199520-mRNA-1:exon:7819;Parent=Msa0199520-mRNA-1
chr2_1 exon 18664486 18664536 18664486 ID=Msa0199520-mRNA-1:exon:7818;Parent=Msa0199520-mRNA-1
chr2_1 five_prime_UTR 18664514 18664536 18664514 ID=Msa0199520-mRNA-1:five_prime_utr;Parent=Msa0199520-mRNA-1
chr2_1 CDS 18664486 18664513 18664486 ID=Msa0199520-mRNA-1:cds;Parent=Msa0199520-mRNA-1
chr2_1 CDS 18663965 18664134 18663965 ID=Msa0199520-mRNA-1:cds;Parent=Msa0199520-mRNA-1
chr2_1 three_prime_UTR 18662545 18663964 18662545 ID=Msa0199520-mRNA-1:three_prime_utr;Parent=Msa0199520-mRNA-1
Gene Sequence

>Msa0199520

ATGAAACCAAAAGACAAATTTGTCAAAGGTCCCAATAAGGAACATCTCATGCATGTGATGAGTCCTAGCAACAAGTTCATCACCACCATGGTCAACCCTTTGAGATTCTGTGACAAAAAAGAAGAAGACATCGGTGGCTCTGAAATTGTAGCTGACAATTATTCAGCAACTTCAGCATTAGTAGCAGGTTCATTCTAA
Protein sequence

>Msa0199520

MKPKDKFVKGPNKEHLMHVMSPSNKFITTMVNPLRFCDKKEEDIGGSEIVADNYSATSALVAGSF*