AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0932190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0932190 MtrunA17_Chr6g0470291 94.898 98 5 0 1 98 1 98 2.80e-56 169
Msa0932190 MtrunA17_Chr7g0251191 53.933 89 41 0 10 98 6 94 2.36e-35 115
Msa0932190 MtrunA17_Chr6g0483691 49.383 81 41 0 18 98 12 92 3.92e-29 100
Msa0932190 MtrunA17_Chr1g0209271 42.222 90 52 0 9 98 3 92 1.21e-26 94.0
Msa0932190 MtrunA17_Chr7g0251171 42.045 88 51 0 10 97 6 93 1.43e-22 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0932190 AT3G18280.2 49.438 89 42 1 10 98 35 120 2.45e-30 104
Msa0932190 AT3G18280.1 49.438 89 42 1 10 98 11 96 4.20e-30 103
Msa0932190 AT1G48750.1 55.556 72 32 0 27 98 23 94 1.47e-28 99.4
Msa0932190 AT1G73780.1 34.694 98 64 0 1 98 1 98 2.78e-22 83.6
Msa0932190 AT1G66850.1 50.725 69 34 0 30 98 34 102 2.02e-21 81.3
Msa0932190 AT1G43666.1 46.154 91 43 3 8 98 11 95 3.82e-20 77.8
Msa0932190 AT5G38170.1 45.588 68 37 0 31 98 36 103 4.62e-19 75.5
Msa0932190 AT5G38195.1 47.761 67 35 0 32 98 29 95 9.17e-19 74.3
Msa0932190 AT1G43667.1 35.000 100 61 2 1 98 1 98 1.87e-18 73.6
Msa0932190 AT5G38160.1 40.260 77 42 1 22 98 31 103 9.75e-18 72.0
Msa0932190 AT5G38180.1 36.000 100 57 2 1 98 1 95 1.09e-17 71.6
Msa0932190 AT2G14846.1 45.161 62 34 0 37 98 38 99 1.31e-14 63.9
Msa0932190 AT1G43665.1 36.250 80 45 2 17 96 22 95 2.29e-14 63.9
Msa0932190 AT1G07747.1 40.909 66 37 1 33 98 29 92 2.30e-13 60.8
Msa0932190 AT5G38197.1 34.694 98 62 2 1 98 1 96 4.62e-13 60.1
Msa0932190 AT3G57310.1 36.364 66 42 0 33 98 38 103 1.05e-12 59.3
Msa0932190 AT4G12825.1 34.066 91 50 2 16 98 7 95 1.67e-12 58.5

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGAGTTCAGTCCTTGTTT+AGG 0.334416 6_3:+172125 Msa0932190:CDS
GTCTCTGCTCTCTTAACTTC+TGG 0.337118 6_3:-172187 None:intergenic
TTTACTCTCCTGGTGCTAGA+AGG 0.339135 6_3:+172255 Msa0932190:CDS
GAGCAGAGACCATGCCTTTG+TGG 0.411218 6_3:+172200 Msa0932190:CDS
GAAGGGTTACTAGCTCTTGT+GGG 0.413579 6_3:+172273 Msa0932190:CDS
TTCTGGCAACAAGCACTTGT+TGG 0.433827 6_3:-172170 None:intergenic
ATGATTGATGGCTAACAAGT+TGG 0.442427 6_3:-172305 None:intergenic
TAAATATACTGCTTGAGGCT+TGG 0.456606 6_3:-172236 None:intergenic
AAACAAGGACTGAACTCTAC+AGG 0.466330 6_3:-172122 None:intergenic
TGGCAACAAGCACTTGTTGG+TGG 0.497936 6_3:-172167 None:intergenic
AGAAGGGTTACTAGCTCTTG+TGG 0.498825 6_3:+172272 Msa0932190:CDS
TGATTGATGGCTAACAAGTT+GGG 0.507677 6_3:-172304 None:intergenic
TGTTTATGCAGTAGTGGTTG+TGG 0.508459 6_3:+172046 Msa0932190:CDS
AAGCAGTATATTTACTCTCC+TGG 0.517554 6_3:+172245 Msa0932190:CDS
TGGTATTGTTTATGCAGTAG+TGG 0.524181 6_3:+172040 Msa0932190:CDS
CTAGTAACCCTTCTAGCACC+AGG 0.531212 6_3:-172263 None:intergenic
TTACTCTCCTGGTGCTAGAA+GGG 0.532631 6_3:+172256 Msa0932190:CDS
GCTTGAGGCTTGGATTCTTG+AGG 0.548134 6_3:-172226 None:intergenic
ATGAAGAAACACTCTAGCTA+TGG 0.561126 6_3:+172020 Msa0932190:CDS
TGATGGCTAACAAGTTGGGA+AGG 0.576643 6_3:-172300 None:intergenic
GAGAGTAAATATACTGCTTG+AGG 0.582416 6_3:-172241 None:intergenic
AAGGGTTACTAGCTCTTGTG+GGG 0.597115 6_3:+172274 Msa0932190:CDS
GGAGGAGACTTACATGGCAA+AGG 0.624840 6_3:+172085 Msa0932190:CDS
AACAAGGACTGAACTCTACA+GGG 0.629235 6_3:-172121 None:intergenic
GATGGCTAACAAGTTGGGAA+GGG 0.649759 6_3:-172299 None:intergenic
TTGAGGTAACCACAAAGGCA+TGG 0.650224 6_3:-172209 None:intergenic
GATTCTTGAGGTAACCACAA+AGG 0.669776 6_3:-172214 None:intergenic
GTTGTGATTGATCCTAAACA+AGG 0.684294 6_3:-172137 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ATGAAGAAACACTCTAGCTA+TGG + chr6_3:172020-172039 Msa0932190:CDS 35.0%
TGGTATTGTTTATGCAGTAG+TGG + chr6_3:172040-172059 Msa0932190:CDS 35.0%
! GTTGTGGCAATAGTGTTTTT+GGG + chr6_3:172062-172081 Msa0932190:CDS 35.0%
!! TTGTGGCAATAGTGTTTTTG+GGG + chr6_3:172063-172082 Msa0932190:CDS 35.0%
GTTGTGATTGATCCTAAACA+AGG - chr6_3:172140-172159 None:intergenic 35.0%
TAAATATACTGCTTGAGGCT+TGG - chr6_3:172239-172258 None:intergenic 35.0%
GAGAGTAAATATACTGCTTG+AGG - chr6_3:172244-172263 None:intergenic 35.0%
AAGCAGTATATTTACTCTCC+TGG + chr6_3:172245-172264 Msa0932190:CDS 35.0%
TGTTTATGCAGTAGTGGTTG+TGG + chr6_3:172046-172065 Msa0932190:CDS 40.0%
! GGTTGTGGCAATAGTGTTTT+TGG + chr6_3:172061-172080 Msa0932190:CDS 40.0%
!!! TGTGGCAATAGTGTTTTTGG+GGG + chr6_3:172064-172083 Msa0932190:CDS 40.0%
AACAAGGACTGAACTCTACA+GGG - chr6_3:172124-172143 None:intergenic 40.0%
AAACAAGGACTGAACTCTAC+AGG - chr6_3:172125-172144 None:intergenic 40.0%
! GTAGAGTTCAGTCCTTGTTT+AGG + chr6_3:172125-172144 Msa0932190:CDS 40.0%
GATTCTTGAGGTAACCACAA+AGG - chr6_3:172217-172236 None:intergenic 40.0%
TTCTGGCAACAAGCACTTGT+TGG - chr6_3:172173-172192 None:intergenic 45.0%
GTCTCTGCTCTCTTAACTTC+TGG - chr6_3:172190-172209 None:intergenic 45.0%
TTGAGGTAACCACAAAGGCA+TGG - chr6_3:172212-172231 None:intergenic 45.0%
! TTTACTCTCCTGGTGCTAGA+AGG + chr6_3:172255-172274 Msa0932190:CDS 45.0%
! TTACTCTCCTGGTGCTAGAA+GGG + chr6_3:172256-172275 Msa0932190:CDS 45.0%
! AGAAGGGTTACTAGCTCTTG+TGG + chr6_3:172272-172291 Msa0932190:CDS 45.0%
! GAAGGGTTACTAGCTCTTGT+GGG + chr6_3:172273-172292 Msa0932190:CDS 45.0%
! AAGGGTTACTAGCTCTTGTG+GGG + chr6_3:172274-172293 Msa0932190:CDS 45.0%
!!! GGCAATAGTGTTTTTGGGGG+AGG + chr6_3:172067-172086 Msa0932190:CDS 50.0%
TTTGGGGGAGGAGACTTACA+TGG + chr6_3:172079-172098 Msa0932190:CDS 50.0%
GGAGGAGACTTACATGGCAA+AGG + chr6_3:172085-172104 Msa0932190:CDS 50.0%
! TGGCAACAAGCACTTGTTGG+TGG - chr6_3:172170-172189 None:intergenic 50.0%
GCTTGAGGCTTGGATTCTTG+AGG - chr6_3:172229-172248 None:intergenic 50.0%
CTAGTAACCCTTCTAGCACC+AGG - chr6_3:172266-172285 None:intergenic 50.0%
GAGCAGAGACCATGCCTTTG+TGG + chr6_3:172200-172219 Msa0932190:CDS 55.0%
Chromosome Type Strat End Strand Name
chr6_3 gene 172020 172316 172020 ID=Msa0932190;Name=Msa0932190
chr6_3 mRNA 172020 172316 172020 ID=Msa0932190-mRNA-1;Parent=Msa0932190;Name=Msa0932190-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|98
chr6_3 exon 172020 172316 172020 ID=Msa0932190-mRNA-1:exon:72;Parent=Msa0932190-mRNA-1
chr6_3 CDS 172020 172316 172020 ID=Msa0932190-mRNA-1:cds;Parent=Msa0932190-mRNA-1
Gene Sequence

>Msa0932190

ATGAAGAAACACTCTAGCTATGGTATTGTTTATGCAGTAGTGGTTGTGGCAATAGTGTTTTTGGGGGAGGAGACTTACATGGCAAAGGCATTGACTTGTAGCCCTGTAGAGTTCAGTCCTTGTTTAGGATCAATCACAACTTCTTCTCCACCAACAAGTGCTTGTTGCCAGAAGTTAAGAGAGCAGAGACCATGCCTTTGTGGTTACCTCAAGAATCCAAGCCTCAAGCAGTATATTTACTCTCCTGGTGCTAGAAGGGTTACTAGCTCTTGTGGGGTTCCCTTCCCAACTTGTTAG

Protein sequence

>Msa0932190

MKKHSSYGIVYAVVVVAIVFLGEETYMAKALTCSPVEFSPCLGSITTSSPPTSACCQKLREQRPCLCGYLKNPSLKQYIYSPGARRVTSSCGVPFPTC*